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Immunomodulatory and Antioxidant Activities of Sulfated Polysaccharides from Laminaria ochroleuca, Porphyra umbilicalis, and Gelidium corneum

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Abstract

Seaweeds of the genera Laminaria, Gelidium, and Porphyra have been used in both food and non-food industries due to their unique properties and characteristic biological activity. This study assesses the antioxidant activity and immunomodulatory properties of the acidic polysaccharides extracted from Laminaria ochroleuca, Porphyra umbilicalis, and Gelidium corneum collected in the Atlantic coast of Tarifa (Cadiz, Spain). The proliferation of murine cell line RAW 264 decreased with increasing concentration of polysaccharides of the three algal species. The highest both antioxidant (25.69 μmol TE g−1 DW) and immunomodulatory activities were observed in the sulfated polysaccharides of L. ochroleuca compared to that of P. umbilicalis and G. corneum. Sulfated polysaccharides of L. ochroleuca presented high potential anticancer activity in cell lines of human colon cancer HTC-116 (IC50 = 0.44 mg mL−1), human malignant melanoma G-361 (IC50 = 5.42 mg mL−1), breast adenocarcinoma human MCF-7 (IC50 = 8.32 mg mL−1), and human leukemia U-937 (IC50 = 3.72 mg mL−1). It is concluded that metabolites of L. ochroleuca can offer significant advantages for the pharmaceutical industry, particularly when macrophage activation is required.

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Acknowledgements

We want to express our gratitude to the Photobiology and Biotechnology of Aquatic Organisms (FYBOA) research group (RNM-295) for their financial support. We also want to thank Dr. Luis Alemany and Dr. María de los Ángeles Vargas (Chemical Engineering Department, UMA) for their technical assistance in the FT-IR analysis and D. Augusto Martínez García, Technical Manager of the Chemical Analysis and Material Characterization Area: Elemental and Thermal Analysis Unit of the SCAI (Central Research Support Service) of the University of Malaga, for the technical assistance in the elementary analysis of the samples.

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Authors and Affiliations

  1. Ecology Department, Faculty of Sciences, Malaga University, Campus de Teatinos s/n, CP 29071, Malaga, Spain

    Roberto T. Abdala Díaz, V. Casas Arrojo, M. A. Arrojo Agudo & F. L. Figueroa

  2. Biochemistry Department, Faculty of Sciences, Malaga University, Campus de Teatinos s/n, CP 29071, Malaga, Spain

    C. Cárdenas

  3. Department of Marine Science and Fisheries, College of Agricultural and Marine Sciences, Sultan Qaboos University, PO Box 34, 123, Al Khoud, Muscat, Oman

    S. Dobretsov

  4. Center of Excellence in Marine Biotechnology, Sultan Qaboos University, PO Box 50, 123, Al Khoud, Muscat, Oman

    S. Dobretsov

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  1. Roberto T. Abdala Díaz
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  2. V. Casas Arrojo
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  3. M. A. Arrojo Agudo
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  4. C. Cárdenas
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  6. F. L. Figueroa
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Correspondence to Roberto T. Abdala Díaz.

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Abdala Díaz, R.T., Casas Arrojo, V., Arrojo Agudo, M.A. et al. Immunomodulatory and Antioxidant Activities of Sulfated Polysaccharides from Laminaria ochroleuca, Porphyra umbilicalis, and Gelidium corneum. Mar Biotechnol 21, 577–587 (2019). https://doi.org/10.1007/s10126-019-09905-x

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