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c-Jun promotes the survival of H9c2 cells under hypoxia via PTEN/Akt signaling pathway

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Abstract

Ischemia and hypoxia are common pathophysiological characteristics in cardiovascular diseases. c-Jun expression could be induced by extra- or intracellular stimuli and plays a pivotal role in regulating cell survival in response to the stress. However, previous studies of c-Jun in cell proliferation and apoptosis showed conflicting results. In the present study, we demonstrated that the expression of c-Jun was induced by hypoxia in H9c2 cells. Loss of function of c-Jun was investigated by CCK-8, LDH, and TUNEL assays in low oxygen (1% O2) conditions. We revealed that c-Jun could promote cell survival and inhibit cell apoptosis under hypoxia. Knockdown of c-Jun also promoted the expression of apoptosis-related proteins under hypoxia, such as cleaved caspase-3, cleaved caspase-9, Bax, and Bim. Furthermore, we demonstrated that the knockdown of c-Jun inhibited the PTEN/Akt signaling pathway under hypoxia. Our findings suggested that c-Jun protected H9c2 cells from apoptosis and promoted the survival of H9c2 cells under hypoxia via PTEN/Akt signaling pathway.

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Funding

The present work was financially supported by the National Natural Science Foundation of China, Grant Nos. 81270228 and 81700277.

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Authors and Affiliations

  1. Department of Cardiovascular Surgery, General Hospital of Western Theater Command, Chengdu, 610083, Sichuan, People’s Republic of China

    Fan Wu, Feng Gao & Siyi He

  2. Department of Cardiovascular Surgery, Xinqiao Hospital, Army Medical University, Xinqiao Street, Shapingba District, Chongqing, 400037, People’s Republic of China

    Fan Wu, Yunhan Jiang, Guiping Luo & Yingbin Xiao

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  1. Fan Wu
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  2. Feng Gao
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  3. Siyi He
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  4. Yunhan Jiang
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Correspondence to Yingbin Xiao.

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Wu, F., Gao, F., He, S. et al. c-Jun promotes the survival of H9c2 cells under hypoxia via PTEN/Akt signaling pathway. J Physiol Biochem 75, 433–441 (2019). https://doi.org/10.1007/s13105-019-00695-3

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  • DOI: https://doi.org/10.1007/s13105-019-00695-3

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