Abstract
The Streptomyces sp. strain AV05 isolated from an organic amendment was found to impact both growth and fumonisin production of Fusarium verticillioides during in vitro direct confrontation. In order to investigate the interactions between the Streptomyces sp. strain AV05 and F. verticillioides, a metabolomic approach was used. The study of the endometabolomes of the microorganisms was carried out in two different conditions: the microorganisms were cultivated alone or in confrontation. The aim of this study was to examine the modifications of the endometabolome of F. verticillioides in confrontation with the Streptomyces strain. The metabolites involved in these modifications were identified using 2D NMR. Many metabolites were found to be overproduced in confrontation assays with the Streptomyces strain, notably 16 proteinogenic amino acids, inosine, and uridine. This suggested that fungal metabolic pathways such as protein synthesis have been affected due to interaction. Thus, metabolomic studies, as well as proteomics or transcriptomics, are useful for deciphering the mechanisms of interactions between biological control agents and mycotoxigenic fungi. This comprehension is one of the key elements of the improvement of the selection and use of antagonistic agents.
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Acknowledgments
Our appreciations are sent to the platform PFEM (Plateforme d’Exploration du Métabolisme-Clermont-Ferrand, France) as well as the platform MétaToul (Plateforme de Métabolomique et Fluxomique de Toulouse-Toulouse, France) for supporting us in the metabolomic techniques.
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Nguyen, PA., Strub, C., Lagrée, M. et al. Study of in vitro interaction between Fusarium verticillioides and Streptomyces sp. using metabolomics. Folia Microbiol 65, 303–314 (2020). https://doi.org/10.1007/s12223-019-00725-z
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DOI: https://doi.org/10.1007/s12223-019-00725-z