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Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato

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Acknowledgements

This work was supported by the French National Research Agency, (ref no. ANR11-BTBR-0001-GENIUS). The authors thank Peter Rogowsky for his efficient management of the GENIUS project and Holger Puchta and the KIT Botanisches Institut for the pDe–CAS9 plasmid. The authors thank Luc Jourdon (GAFL Unit) for his technical support for in vitro culture. The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS).

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Correspondence to Marianne Mazier.

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Communicated by Neal Stewart.

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Danilo, B., Perrot, L., Mara, K. et al. Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato. Plant Cell Rep 38, 459–462 (2019). https://doi.org/10.1007/s00299-019-02373-6

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  • DOI: https://doi.org/10.1007/s00299-019-02373-6

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