Abstract
The amino acid/auxin permease (AAAP) gene family plays an important role in the long-distance amino acid transport pathway and takes part in various stages of plant growth and development. However, little is known about the AAAP gene family in Medicago truncatula. Here, we identified 86 putative MtAAAP family members using genome sequence information. Based on phylogenetic analysis, these MtAAAP genes were categorized into eight distinct subfamilies. The MtAAAP genes were mapped on 8 chromosomes and duplication events appeared widely, with 19 and 21 pairs of MtAAAP genes showing segment and tandem duplication events, respectively. Ratio of Ka/Ks indicated that duplicated genes underwent purifying selection. Analysis of RNA-seq data showed that MtAAAP genes exhibited specific expression patterns among different tissues and abiotic stress, indicating that MtAAAP members were involved in plant developmental regulation and stress responses. Expression patterns of 16 MtAAAP genes under abiotic stress were verified by qRT-PCR. The present study provides a foundation for the functional analysis of MtAAAPs in developmental regulation and stress responses.
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This work was supported by the grant from the National Nature Science Foundation of China (nos. 31470571; 31770575). The National Major Project for Cultivation of Transgenic Crops (#2016ZX08004-002-003), and The National Key Research and Development Program of China (2017YFD0101303).
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10709_2019_62_MOESM1_ESM.tif
Fig. S1 Phylogenetic tree analysis of the AAAP family in Medicago truncatula, Arabidopsis thaliana and Oryza sativa L (TIF 1648 KB)
10709_2019_62_MOESM3_ESM.tif
Fig. S3 Multiple sequence alignment and transmembrane region of LHT subfamily in Medicago truncatula. Identical (100%), conservative (75-99%), and block (50-74%) of similar amino acid residues are shaded in deep, lilac, and Cambridge blue, respectively. The transmembrane regions are marked by gray lines. The conserved motifs are marked by yellow lines (TIF 4888 KB)
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Qu, Y., Ling, L., Wang, D. et al. Genome-wide identification and expression analysis of the AAAP family in Medicago truncatula. Genetica 147, 185–196 (2019). https://doi.org/10.1007/s10709-019-00062-6
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DOI: https://doi.org/10.1007/s10709-019-00062-6