Elsevier

Photoacoustics

Volume 14, June 2019, Pages 12-18
Photoacoustics

Research article
The integrated high-resolution reflection-mode photoacoustic and fluorescence confocal microscopy

https://doi.org/10.1016/j.pacs.2019.02.001Get rights and content
Under a Creative Commons license
open access

Abstract

A dual modality microscopy with the highest imaging resolution reported so far based on reflection-mode photoacoustic and confocal fluorescence is presented in this study. The unique design of the imaging head of the microscope makes it highly convenient for scalable high-resolution imaging by simply switching the optical objectives. The submicron resolution performance of the system is demonstrated via in vivo imaging of zebrafish, normal mouse ear, and a xenograft tumor model inoculated in the mouse ear. The imaging results confirm that the presented dual-modality microscopy imaging system could play a vital role in observing model organism, studying tumor angiogenesis and assessment of antineoplastic drugs.

MSC

00-01
99-00

Keywords

Photoacoustic microscopy
Confocal microscopy
Multimodal imaging

Cited by (0)

Chengbo Liu is an Associate Professor at SIAT, CAS. He received both his Ph.D. and Bachelor degree from Xi’an Jiaotong University, each in 2012 in Biophysics and 2007 in Biomedical Engineering. During his Ph.D. training, he spent two years doing tissue spectroscopy research at Duke University as a visiting scholar. Now he is an associate professor at SIAT, working on multi-scale photoacoustic imaging and its translational research.

Jiuling Liao received his B.S. degree in Photoelectric Information Engineering from Nanjing University of Science and Technology in 2012, and Ph.D. degree in Optical Engineering from Nanjing University of Science and Technology in 2017. Dr. Liao joined SIAT as a Postdoctor in July 2017. His research interest focuses on the application of multimodal microscopy.

Longchao Chen received his Master's degree in Optical Engineering from Fujian Normal University, Ministry of Education Key Laboratory of OptoElectronic Science and Technology for Medicine in 2014. His current interest focuses on designing and researching Laser scanning confocal microscopy and super-resolution microscopy system.

Jianhua Chen received his Master's degree in Electronic Engineering from Tsinghua University on the topic of ultra-high speed optical fiber transmission and signal processing. His current research focuses on developing data acquisition and imaging schemes for AR/OR-Photoacoustic medical devices.

Rubo Ding received his B.S. degree from Zhejiang University. His research interest focuses on developing new model medical equipment.

Xiaojing Gong is an Associate Professor at SIAT, CAS. He received his B.S. degree in Mechatronics from the Southeast University in 2000, and Ph.D. degree in Fine Instruments and Mechanics from the University of Science and Technology of China in 2007. Since joined the IBHE of SIAT in 2007, Dr. Gong focuses in the research of Biomedical Optics Imaging, includes Photoacoustic Tomography/Microscopy, Optical Coherence Tomography/Microscopy, Terahertz biomedical imaging, etc.

Caimei Cui received her B.S. degree in Biotechnology from Xiamen University, and Mater degree in Medical Biochemistry and Molecular Biology Sun Yat their-sen University. Now she focuses on translational clinical research.

Zhiqiang Pang received his Master's degree in Engineering Management from Tsinghua University. He focuses on translating research discoveries from the laboratory into clinical practice to diagnose and treat patients.

Wei Zheng is a professor at SIAT, CAS. He received his B.S. degree in Optical Engineering from Zhejiang University in 2003, his Ph.D. degree in Electronic and Computer Engineering from Hong Kong University of Science and Technology in 2011. His research focuses on biomedical optics, including fluorescence spectroscopy, fluorescence microscopy and nonlinear optical microscopy.

Liang Song is a professor at SIAT, CAS and founding directors of The Research Lab for Biomedical Optics, and The Shenzhen Key Lab for Molecular Imaging. Prior to joining SIAT, he studied at Washington University, St. Louis and received his Ph.D. in Biomedical Engineering. His research focuses on multiple novel photoacoustic imaging technologies.

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These authors contributed equally to this work.