Abstract
Anoplophora glabripennis (Motschulsky) has an advanced and complicated olfactory system to identify hosts, mates and spawning locations, and odorant-binding proteins (OBPs) play a key role by binding to volatile materials from different hosts. The full-length cDNA sequence of an OBP, AglaOBP, was cloned by RACE from an antenna cDNA library, and the protein structure and function were predicted by bioinformatics analysis. Gene temporal and spatial expression was detected by real-time qPCR. AglaOBP had distinctive sequence, location and expression profiles compared with other OBPs of A. glabripennis, as it was found in different tissues, and the highest expression was in the elytrums. The possible physiological functions of this OBP were discussed. These findings help elucidate the physiology of this pest and provide a new potential target for pest control.
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Acknowledgements
Thanks for the technical support of the Key Laboratory of Genetic Resources of Forest and Forest Protection of Hebei. And Thanks for AJE’s help in language modification about this article. This work was supported by the Hebei Province Natural Science Foundation—Youth Foundation (Grant No. C2012204098).
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Yu Li and Huien Li were equally contributed to the work.
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The datasets generated and analysed during the current study are available from the corresponding author on reasonable request.
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Li, Y., Li, H., Wang, Z. et al. Cloning, localization and bioinformatics analysis of a gene encoding an odorant-binding protein (OBP) in Anoplophora glabripennis (Motschulsky). Invert Neurosci 18, 11 (2018). https://doi.org/10.1007/s10158-018-0215-5
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DOI: https://doi.org/10.1007/s10158-018-0215-5