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Roles of 5-HT on phase transition of neurite outgrowth in the identified serotoninergic neuron C1, Helisoma trivolvis

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Invertebrate Neuroscience

Abstract

Neurite outgrowth is a morphological marker of neuronal differentiation and neuroregeneration, and the process includes four essential phases, namely initiation, elongation, guidance and cessation. Intrinsic and extrinsic signaling molecules seem to involve morphological changes of neurite outgrowth via various cellular signaling cascades phase transition. Although mechanisms associated with neurite outgrowth have been studied extensively, little is known about how phase transition is regulated during neurite outgrowth. 5-HT has long been studied with regard to its relationship to neurite outgrowth in invertebrate and vertebrate culture systems, and many studies have suggested 5-HT inhibits neurite elongation and growth cone motility, in particular, at the growing parts of neurite such as growth cones and filopodia. However, the underlying mechanisms need to be investigated. In this study, we investigated roles of 5-HT on neurite outgrowth using single serotonergic neurons C1 isolated from Helisoma trivolvis. We observed that 5-HT delayed phase transitions from initiation to elongation of neurite outgrowth. This study for the first time demonstrated that 5-HT has a critical role in phase-controlling mechanisms of neurite outgrowth in neuronal cell cultures.

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Abbreviations

5-HT:

Hydroxytryptamine

TpOH:

Tryptophan hydroxylase

C1:

Neuron (the cerebral ganglion neuron C1)

CM:

Conditioned media

DM:

Defined media

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Acknowledgements

This work was supported by a kind donation from Envirobrain. M.S. Song is currently an assistant professor in the Department of Biological Sciences, Dankook University (DKU). Glen B Baker is a vice chair for research at University of Alberta. K.C. Ahn is a chief researcher in Envirobrain. We acknowledge Jung H. Ahn for correcting grammatical errors in the manuscript.

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Authors and Affiliations

  1. Neurochemical Research Unit, Department of Psychiatry, University of Alberta, Edmonton, AB, Canada

    Kee-Chan Ahn & Glen B. Baker

  2. Envirobrain, Edmonton, Canada

    Kee-Chan Ahn

  3. Department of Chemistry, Dankook University, Cheonan, South Korea

    Won-Cheoul Jang

  4. Department of Biological Sciences, Dankook University, Cheonan, Chungbuk, South Korea

    Hyeon-Cheol Cha, Myung Jin Moon & Mee-Sook Song

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  1. Kee-Chan Ahn
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Correspondence to Kee-Chan Ahn or Mee-Sook Song.

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Ahn, KC., Baker, G.B., Jang, WC. et al. Roles of 5-HT on phase transition of neurite outgrowth in the identified serotoninergic neuron C1, Helisoma trivolvis. Invert Neurosci 18, 10 (2018). https://doi.org/10.1007/s10158-018-0214-6

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