Abstract
Anin vitro selection system using microcross sections of banana and plantain cultivars belonging to AAA and AAB genomic groups were used to produce plants resistant against the Black Sigatoka disease. The fungus resistant plantlets were obtained in a double selection system. This involved in a first step the use of a fungal crude filtrate and in the second step the purified host-specific toxin 2,4,8-trihydroxytetralone extracted from the fungusMycosphaerella fijiensis (M. fijiensis), the causal agent of Black Sigatoka disease. Resistant plantlets obtained from the double selection system were inoculated with conidia ofM. fijiensis in a growth chamber to reproduce Black Sigatoka symptoms. Compared to non-treated control plantlets, which were highly susceptible to the fungus, 10.7–19.3% toxin-resistant plantlets which arose from tissues that went through the double selection system were resistant againstM. fijiensis. This technique of using micro-cross sections for selection on fungal toxins seems to be amenable to differentMusa genotypes for the production of fungus-resistant plants.
Similar content being viewed by others
References
Cartier J, Gonzalez de Leon D, Mourichon X, Zapater M (1993) In: Ganry J (ed) Breeding banana and plantain for resistance to diseases and pests. CIRAD/INIBAP, Montpellier, France, pp 193–199.
Chandler SF, Thorpe TA (1987) Plant Cell Rep 9: 611–614.
Gentile A, Tribulato E, Continella G, Vardi A (1992) Theor Appl Genet 83: 759–764.
Hartman CL, McCoy TJ, Knous TR (1984) Plant Science Letters 34: 195–201.
Mobambo K, Gauhl F, Vuylsteke D, Ortiz R, Pasberg-Gauhl C, Swennen R (1993) Field Crops Research 35: 35–42.
Mourichon X, Peter D, Zapater M (1987) Fruits 2: 195–98.
Müller R, Pasberg-Gauhl C, Gauhl F, Kaemmer D, Kahl G (1995) InfoMusa, INIBAP, Montpellier, France 4:9–11.
Murashige T, and Skoog F (1962) Physiol Plant 15: 473–497.
Okole BN, Fisher ES, Schutz FA (1994) Proceedings of the German Botanical Congress, 11–19 Sept. 1994, Bayreuth, Germany, p. 369.
Okole BN, Schulz FA (1996) Plant Science 116: 185–195.
Pinkerton F, Strobel G (1976) Proc of the National Academy of Science, U.S.A. 74: 4007–4011.
Stierle A, Upadhyay R, Hershenhorn J, Strobel G, Molina G (1991) Experientia 47: 853–859.
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
F. A. Schulz died 11. 3. 1995
Rights and permissions
About this article
Cite this article
Okole, B.N., Schulz, F.A. Selection ofMycosphaerella fijiensis-resistant cell lines from micro-cross sections of banana and plantain. Plant Cell Reports 16, 339–343 (1997). https://doi.org/10.1007/BF01088293
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01088293