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The identification and distribution of gonadotropin-releasing hormone-like peptides in the central nervous system and ovary of the giant freshwater prawn, Macrobrachium rosenbergii

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Invertebrate Neuroscience

Abstract

In the present study, we demonstrated the existence of GnRH-like peptides in the central nervous system (CNS) and ovary of the giant freshwater prawn, Macrobrachium rosenbergii using immunocytochemistry. The immunoreactivity (ir) of lamprey (l) GnRH-III was detected in the soma of medium-sized neurons located in neuronal cluster number 11 in the middle part of supraesophageal ganglion (deutocerebrum), whereas ir-octopus (oct) GnRH was observed in the soma of both medium-sized and large-sized neurons in thoracic ganglia, as well as in the fibers innervating the other medium-sized and large-sized neuronal cell bodies in the thoracic ganglia. In addition, ir-lGnRH-I was observed in the cytoplasm of late previtellogenic oocyte and early vitellogenic oocyte. These data suggest that M. rosenbergii contain at least three isoforms of GnRH: two GnRH isoforms closely related to lGnRH-III and octGnRH in the CNS, whereas another isoform, closely related to lGnRH-I, was localized in the ovary. This finding provides supporting data that ir-GnRH-like peptide(s) may exist in this decapod crustacean.

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Acknowledgements

This study was supported by the Thailand Research Fund (Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0249/2546) to A. Ngernsoungnern and P. Sretarugsa, and Senior Research Scholar Fellowship to P. Sobhon), Commission on Higher Education, Ministry of Education (Research Group Development Grant to P. Sobhon) and NSF0421923 grant to Stacia A. Sower. The authors would like to thank Dr. Pei-San Tsai, Dr. Judy King, and Dr. Hiroyuki Minakata for generously providing GnRH antisera and peptides.

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Correspondence to Prapee Sretarugsa.

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Ngernsoungnern, A., Ngernsoungnern, P., Kavanaugh, S. et al. The identification and distribution of gonadotropin-releasing hormone-like peptides in the central nervous system and ovary of the giant freshwater prawn, Macrobrachium rosenbergii . Invert Neurosci 8, 49–57 (2008). https://doi.org/10.1007/s10158-008-0067-5

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  • DOI: https://doi.org/10.1007/s10158-008-0067-5

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