Expression of Robo/Slit and Semaphorin/Plexin/Neuropilin family members in the developing hypothalamic paraventricular and supraoptic nuclei
Section snippets
Results and discussion
Birth dating experiments in rodents have assigned the origin of the PVN and SON to the anterior ventral lobes of the diencephalon (Altman and Bayer, 1978). They are derived from a common progenitor origin (i.e. the PVN/SON progenitor), but segregate into two distinct nuclei distant from each other. The mature PVN contains both magnocellular (large-body) and parvocellular (small-body) hormone-producing neurons, while the mature SON contains only the magnocellular neurons (Swanson, 1986,
Experimental animals
Mice of BL6/129sv mixed genetic background were used for mating. The vaginal plug date is assigned as E0.5. The anatomical nomenclatures are based on the rat brain atlas by Altman and Bayer (1995). All animal experimentation described herein was conducted in accordance with accepted standards of humane animal care outlined in Ethical Guidelines and approved by IACUC.
In situ hybridization (ISH)
Whole embryo heads were fixed in Methancarn (60% methanol, 30% chloroform, and 10% acetic acid), dehydrated in methanol, embedded
Acknowledgments
We thank Drs. K. Brose, A. Kolodkin, L. Puelles and J. Rubenstein for the plasmids to generate probes for ISH. We also thank Fan lab members for critical readings of the manuscript. This work is supported by National Institutes of Health Grant RO1 HD35596 (to C.M.F.).
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