Identification of a novel transcriptional corepressor, Corl2, as a cerebellar Purkinje cell-selective marker
Section snippets
Results and discussion
The cerebellum controls movement and posture, and is mainly composed of one glutamatergic neuronal subtype (granule cells) and four GABAergic subtypes (Purkinje, Golgi, stellate and basket cells) (Wang and Zoghbi, 2001). Purkinje cells (PCs) provide the primary output from the cerebellar cortex, and loss of PCs causes severe cerebellar dysfunction (Sidman, 1983, Sotelo, 2004). Although the mechanism of PC differentiation during later developmental stages has been well studied (Gold et al., 2003
Isolation of Corl2
A full-length cDNA for Corl2 was screened from an E12.5 mouse brain cDNA library and sequenced (Mizuhara et al., 2005). The nucleotide sequence of the Corl2 cDNA was deposited in the DDBJ/EMBL/GenBank database under Accession No. AB358976.
RT-PCR
RT-PCR was performed essentially as described previously (Mizuhara et al., 2005). ExTaq polymerase (TAKARA) was used for amplification, which was carried out by denaturation at 94 °C for 30 s (2 min in the first cycle), annealing at 65 °C for 30 s and extension at
Acknowledgements
We are grateful to Dr. T. Imai (KAN Research Institute Inc.) for helpful comments and encouragement. The monoclonal anti-Lhx1/5 antibody was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained at the Department of Biological Sciences, The University of Iowa, Iowa City, IA 52242.
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These authors contributed equally to this work.