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Microscopy techniques for investigating the control of organic constituents on biomineralization

  • Biomineralization
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Abstract

This article addresses recent advances in the application of microscopy techniques to characterize crystallization processes as they relate to biomineralization and bioinspired materials synthesis. In particular, we focus on studies aimed at revealing the role organic macromolecules and functionalized surfaces play in modulating the mechanisms of nucleation and growth. In nucleation studies, we explore the use of methods such as in situ transmission electron microscopy, atomic force microscopy, and cryogenic electron microscopy to delineate formation pathways, phase stabilization, and the competing effects of free energy and kinetic barriers. In growth studies, we emphasize understanding the interactions of macromolecular constituents with growing crystals and characterization of the internal structures of the resulting composite crystals using techniques such as electron tomography, atom probe tomography, and vibrational spectromicroscopy. Examples are drawn from both biological and bioinspired synthetic systems.

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Acknowledgements

The authors acknowledge support from the National Science Foundation (DMR 1210304) and the National Institutes of Health (CA173083). J.J.D.Y. and J.T. acknowledge support from the US Department of Energy, Office of Basic Energy Sciences, Division of Materials Science and Engineering J.J.D.Y also acknowledges support from the Laboratory Directed Research and Development Initiative on Materials Synthesis and Simulation across Scales at the Pacific Northwest National Laboratory (PNNL). PNNL is operated by Battelle for the US Department of Energy under Contract DE-AC05–76RL01830.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Cornell University, USA

    Coit T. Hendley IV

  2. Physical Sciences Division, Pacific Northwest National Laboratory, USA

    Jinhui Tao

  3. Department of Materials Science and Engineering, Cornell University, USA

    Jennie A. M. R. Kunitake

  4. Physical Sciences Division, Pacific Northwest National Laboratory; and Department of Materials Science and Engineering and Department of Chemistry, University of Washington, USA

    James J. De Yoreo

  5. Department of Materials Science and Engineering, Cornell University, USA

    Lara A. Estroff

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  1. Coit T. Hendley IV
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Hendley, C.T., Tao, J., Kunitake, J.A.M.R. et al. Microscopy techniques for investigating the control of organic constituents on biomineralization. MRS Bulletin 40, 480–489 (2015). https://doi.org/10.1557/mrs.2015.98

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