- 作 者: ( "xiangsong chen" OR "xiang-song chen" )
-
Plant-specific histone deacetylases associate with ARGONAUTE4 to promote heterochromatin stabilization and plant heat tolerance.New Phytologist (IF 10.323) Pub Date : 2023-02-01 ,DOI:10.1111/nph.18729Fangfang Yang,Yingnan Sun,Xiaoxuan Du,Zhaohui Chu,Xuehua Zhong,Xiangsong ChenHigh temperature causes devasting effects on many aspects of plant cells and thus enhancing plant heat tolerance is critical for crop production. Emerging studies have revealed the important roles of chromatin modifications in heat stress responses. However, how chromatin is regulated during heat stress remains unclear. We show that heat stress results in heterochromatin disruption coupled with histone ...
-
Supplementation of Weizmannia coagulans BC2000 and Ellagic Acid Inhibits High-Fat-Induced Hypercholesterolemia by Promoting Liver Primary Bile Acid Biosynthesis and Intestinal Cholesterol Excretion in Mice.Microorganisms (IF 4.926) Pub Date : 2023-01-19 ,DOI:10.3390/microorganisms11020264Long Jin,Hongyang Dang,Jinyong Wu,Lixia Yuan,Xiangsong Chen,Jianming Yao
The probiotic Weizmannia coagulans (W. coagulans) BC2000 can increase the abundance of intestinal transforming ellagic acid (EA) bacteria and inhibit metabolic disorders caused by hyperlipidemia by activating liver autophagy. This study aimed to investigate the inhibitory effects of W. coagulans BC2000 and EA on hyperlipidemia-induced cholesterol metabolism disorders. C57BL/6J mice (n = 10 in each
...
-
Gamma-Aminobutyric Acid-Producing Levilactobacillus brevis Strains as Probiotics in Litchi Juice Fermentation.Foods (IF 5.561) Pub Date : 2023-01-08 ,DOI:10.3390/foods12020302Yiwen Jin,Jinyong Wu,Dan Hu,Jun Li,Weiwei Zhu,Lixia Yuan,Xiangsong Chen,Jianming Yao
Levilactobacillus brevis strains can be isolated from traditional Chinese pickles and used as the starter cultures to improve the nutritional profiles of fermented juices. Three L. brevis strains (LBG-29, LBG-24, LBD−14) that produce high levels of gamma-aminobutyric acid (GABA; >300 mg/L) were isolated from traditional Chinese pickles. The strains showed tolerance to low pH and high bile salts and
...
-
The value of concentration of alveolar nitric oxide in diagnosing small airway dysfunction in patients with stable asthmaClinical Respiratory Journal (IF 1.761) Pub Date : 2022-12-12 ,DOI:10.1111/crj.13565Jing Wang, Ke Wu, Xianliang Cheng, Xiangsong Chen, Yanan Qi, Limin ZhaoExhaled nitric oxide (FeNO) is a simple, noninvasive, and reproducible test, and FeNO (50 ml/s) is often used to reflect airway inflammation. The peripheral small airway/alveolar nitric oxide (NO) concentration is derived from the output of NO at multiple flow rates. Concentration of alveolar NO (CANO), which has been reported to reflect peripheral small airway inflammation, may be related to parameters ...
-
Carboxymethyl chitosan/N-acetylneuraminic acid/oxidised hydroxyethyl cellulose hydrogel as a vehicle for Pediococcus pentosaceus RQ-1 with isomaltose-oligosaccharide: Enhanced in vitro tolerance and storage stability of probioticLWT - Food Science and Technology (IF 6.056) Pub Date : 2022-11-29 ,DOI:10.1016/j.lwt.2022.114236Yuanfei Ge, Jinyong Wu, Min Pang, Weiwei Zhu, Lijie Sun, Lixia Yuan, Xiangqin Wang, Xiangsong Chen, Jianming Yao
C-NeuAc/OHEC hydrogels were prepared from carboxymethyl chitosan/N-acetylneuraminic acid (C-NeuAc) and oxidised hydroxyethyl cellulose (OHEC), and used as novel vehicles for the co-encapsulation of Pediococcus pentosaceus RQ-1 and isomaltose-oligosaccharide (IMO). The thermal stability, rheological behaviour, swelling kinetics, porosity, pH sensitivity, and degradability of the hydrogels were evaluated
...
-
Recent EAST Experimental Results and Systems Upgrade in Support of Long-Pulse Steady-State Plasma OperationIEEE Transactions on Plasma Science (IF 1.368) Pub Date : 2022-10-31 ,DOI:10.1109/tps.2022.3210124Cheng, Wendong Ma, Lianmin Zhao, Liang Liu, Bojiang Ding, Huaichuan Hu, Weiye Xu, Dajun Wu, Yong Yang, Mingfu Wang, Jiaxing Li, Xuetian Liu, Lili Zhu, Yiyun Huang, Yu Wu, Xiang Gao, Xiaojie Wang, Xiangsong Chen, Guangnan Luo, Yuanlai Xie, Lei YangIn recent years, EAST has achieved a 60-s scale steady-state plasma operation (SSO), which is electron dominant heated by the electron cyclotron wave and low hybrid wave. Substantial progresses have been made in the development of long-pulse operation, such as active control of radiative divertor, edge localized mode (ELM) suppression, and He plasma experiment. To further develop the steady-state operation ...
-
Weizmannia coagulans BC2000 Plus Ellagic Acid Inhibits High-Fat-Induced Insulin Resistance by Remodeling the Gut Microbiota and Activating the Hepatic Autophagy Pathway in MiceNutrients (IF 4.546) Pub Date : 2022-10-09 ,DOI:10.3390/nu14194206Long Jin, Hongyang Dang, Jinyong Wu, Lixia Yuan, Xiangsong Chen, Jianming Yao
(1) Background: Ellagic acid (EA) acts as a product of gut microbiota transformation to prevent insulin resistance, which is limited by high-fat diet (HFD)-induced dysbiosis. The aim of this study was to investigate the synergistic effects and mechanisms of supplementation with the probiotic Weizmannia coagulans (W. coagulans) on the prevention of insulin resistance by EA; (2) Methods: C57BL/6J mice
...
-
Gravity-induced geometric spin Hall effect of freely falling quantum particleCommunications in Theoretical Physics (IF 2.877) Pub Date : 2022-09-26 ,DOI:10.1088/1572-9494/ac7e2dZhen-Lai Wang, Xiang-Song ChenWe discuss a new gravitational effect that the wave packet of a free-fall quantum particle undergoes a spin-dependent transverse shift in Earth’s gravitational field. This effect is similar to the geometric spin Hall effect (GSHE) (Aiello 2009 et al Phys. Rev. Lett. 103 100401 ), and can be called gravity-induced GSHE. This effect suggests that the free-fall wave packets of opposite spin-polarized ...
-
Protein engineering of CMP kinases to improve thermal stability and resultant production of 3′-sialyllactoseBiotechnology & Biotechnological Equipment (IF 1.762) Pub Date : 2022-07-05 ,DOI:10.1080/13102818.2022.2095302Jingjing Wang, Jinyong Wu, Zhongkui Li, Xiangsong Chen, Weiwei Liu, Jianming Yao
Abstract Protein engineering is an effective method to improve the thermal stability of biological macromolecules. Cytidine monophosphate kinase (CMK) is a key enzyme involved in nucleotide metabolism and participates in the production of cytidine triphosphate. It has previously been confirmed that CMK shows low thermal stability. This study aimed to enhance the thermal stability of CMK. Herein, we
...
-
Replication protein RPA2A regulates floral transition by cooperating with PRC2 in ArabidopsisNew Phytologist (IF 10.323) Pub Date : 2022-05-28 ,DOI:10.1111/nph.18279Xiaoling Zhang, Wenjuan Li, Yue Liu, Yanzhuo Li, Yang Li, Wandong Yang, Xiangsong Chen, Limin Pi, Hongchun YangRPA2A is a subunit of the conserved heterotrimeric replication protein A (RPA) in Arabidopsis, which is an essential replisome component that binds to single-stranded DNA during DNA replication. RPA2A controls a set of developmental processes, but the underlying mechanism is largely unknown. ...
-
Effect of isomaltose oligosaccharide on the freeze-drying process and shelf-life of Pediococcus pentosaceusLWT - Food Science and Technology (IF 6.056) Pub Date : 2022-05-22 ,DOI:10.1016/j.lwt.2022.113583Dan Hu, Jinyong Wu, Jun Li, Long Jin, Xiangsong Chen, Jianming Yao
Improvement in the survival of the edible probiotic Pediococcus pentosaceus during the preparation and storage is important. In this study, the protective effects of isomaltose oligosaccharide in a protective agent (sodium glutamate, skimmed milk, and trehalose) on P. pentosaceus during vacuum freeze-drying and low-temperature storage were evaluated. Cell damage, and texture were evaluated using fluorescence
...
-
Ethyl levulinate production from cellulose conversion in ethanol medium over high-efficiency heteropoly acidsFuel (IF 8.035) Pub Date : 2022-05-21 ,DOI:10.1016/j.fuel.2022.124642Yishuang Wang, Jingjing Shi, Xiangsong Chen, Mingqiang Chen, Jun Wang, Jianming YaoCellulose conversion for ethyl levulinate (CCEL) production is a potential technology for obtaining liquid fuels. Combined the inherent characteristics of cellulose with “homogeneous reaction-heterogeneous separation” feature of heteropoly acids (HPAs), HPAs catalyzed CCEL under ethanol medium were investigated. Various characterizations confirmed HSiW had appropriate Brønsted and Lewis acid sites ...
-
Gauge dependence of spontaneous radiation spectrum in a time-dependent relativistic non-perturbative Coulomb fieldThe European Physical Journal D (IF 1.611) Pub Date : 2022-05-16 ,DOI:10.1140/epjd/s10053-022-00407-5Xue-Nan Chen, Yu-Hang Luo, Xiang-Song Chen
Abstract Lamb triggered a continuous debate on the gauge choice for atomic interactions with electromagnetic fields, particularly with plane waves and the vacuum field. Modern technologies of Rydberg atoms and relativistic atomic beams make it possible to explore interactions with a more intriguing non-perturbative, adiabatic Coulomb field. In such cases, one would face the well-known tricky issue
...
-
Novel carboxymethyl chitosan/N-acetylneuraminic acid hydrogel for the protection of Pediococcus pentosaceusFood Research International (IF 7.425) Pub Date : 2022-05-14 ,DOI:10.1016/j.foodres.2022.111355Yuanfei Ge, Jinyong Wu, Min Pang, Dan Hu, Zhongkui Li, Xiangqin Wang, Lijie Sun, Xiangsong Chen, Jianming YaoIn this study, carboxymethyl chitosan (CMCS) and N-acetylneuraminic acid (NeuAc) were used to develop C-NeuAc hydrogels to encapsulate Pediococcus pentosaceus QK-1. The mechanical properties, thermal stability, in vitro degradation, and pH sensitivity of the hydrogel were evaluated. The C-NeuAc concentration required for optimal hydrogel performance was 3% (w/v). Hydrogel swelling behaviour was effectively ...
-
Birefringence of wave packets in gravityPhysical Review D (IF 5.407) Pub Date : 2022-05-05 ,DOI:10.1103/physrevd.105.104008Dan-Dan Lian, Xiang-Song ChenIn most experiments to test the weak equivalence principle, the test objects coupled with gravity are treated as point particles. To study whether the weak equivalence principle is obeyed by nonlocal objects, especially wave packets with interesting physical configurations, one must first properly define the trajectory of such objects. A phenomenon called the geometric spin Hall effect suggests that ...
-
Repeatedly readable state, spontaneous collapse, and quantum/classical boundaryarXiv - PHYS - Quantum Physics Pub Date : 2022-04-25 ,DOI:arxiv-2204.11656Xiao-Fu Peng, Yu-Hang Luo, Jiang Zhu, Bang-Hui Hua, Xue-Nan Chen, Dan-Dan Lian, Zi-Wei Chen, Xiang-Song ChenWe propose a model to identify the quantum/classical boundary. The model introduces a spontaneous collapse of state superposition: $\frac{d}{dt} \rho_{ij} =-\frac{i}{\hbar}[H,\rho]_{ij}-\rho_{ij}/\tau_{ij}$. Different from other collapse models, the collapsing scale $\tau_{ij}$ here does not contain a universal parameter, but is specified by the two states $| i\rangle $ and $ | j\rangle$: If each state ...
-
Polyoxometalates as Potential Next-Generation Metallodrugs in the melanogenesis inhibitorZeitschrift für anorganische und allgemeine Chemie (IF 1.414) Pub Date : 2022-03-13 ,DOI:10.1002/zaac.202100319Xiangsong Chen, Die Shuai, Han Yang, Dan Luo, Li Wang, Bingnian ChenMelanogenesis is a process to synthesize melanin, which is a primary responsibility for the pigmentation of human skin, eye and hair. In order to study the effect of polyoxometalates on tyrosinase activity and melanin content in mouse melanocytes, four polyoxometalates with Dawson structure were synthesized. Intracellular and docking studies have shown that the two transition metal phosphomolybdic ...
-
Study on the Regulation and Mechanism of the Vanadium Substituted Polyoxometalates of H6[P2Mo18O62] on Melanogenesis of Mouse Melanoma Cell B16Acta Chimica Sinica (IF 2.789) Pub Date : 2022-01-01 ,DOI:10.6023/a21110528Xiangsong Chen,Die Shuai,Zedong Jiang,Han Yang,Dan Luo,Hui Ni,Li Wang,Bingnian Chen
-
Preparation, characterisation and antibacterial activity evaluation of N-acetylneuraminic acid-crosslinked chitosan hydrogelsPolymer Testing (IF 4.931) Pub Date : 2021-12-26 ,DOI:10.1016/j.polymertesting.2021.107457Yuanfei Ge, Xiangsong Chen, Xiangqin Wang, Lixia Yuan, Jinyong Wu, Jianming Yao
In this study, CS-NeuAc hydrogel was prepared from chitosan (CS) and N-acetylneuraminic acid (NeuAc) using cyclic freeze/thaw under 1-ethyl-3-(3-dimethylaminopropyl)-carbodiiminde/N-hydroxysuccinimide catalysis. Fourier transformed infrared spectroscopy, X-ray diffraction, and scanning electron microscopy analyses confirmed the successful synthesis of CS-NeuAc hydrogel. CS-NeuAc hydrogels were found
...
-
Evaluation of Pediococcus pentosaceus strains as probiotic adjunct cultures for soybean milk post-fermentationFood Research International (IF 7.425) Pub Date : 2021-06-30 ,DOI:10.1016/j.foodres.2021.110570Dan Hu, Jinyong Wu, Long Jin, Lixia Yuan, Jun Li, Xiangsong Chen, Jianming YaoSoybean milk is an economical substitute for dairy products. Pediococcus pentosaceus has been used as a food additive to improve taste, nutrition, and food safety. In this study, four P. pentosaceus strains (CICC 24444, QK-1, MQ-1 and RQ-1) isolated from various food sources and known to exhibit broad-spectrum antibacterial activities were used to ferment soybean milk, and their fermentation characteristics ...
-
Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway.Bioscience, Biotechnology, and Biochemistry (IF 2.337) Pub Date : 2021-06-24 ,DOI:10.1093/bbb/zbab074Zhijian Ni,Jinyong Wu,Zhongkui Li,Lixia Yuan,Yu Wang,Xiangsong Chen,Jianming Yao3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use in infant formula. However, development of 3-FL has been somewhat sluggish due to its low content in human milk and poor yield in enlarged production. Here, an α-1,3-fucosyltransferase mutant was introduced into an engineered ...
-
IPA1 Negatively Regulates Early Rice Seedling Development by Interfering with Starch Metabolism via the GA and WRKY PathwaysInternational Journal of Molecular Sciences (IF 4.556) Pub Date : 2021-06-20 ,DOI:10.3390/ijms22126605Yonggang He, Menghao Zhu, Zhihui Li, Shan Jiang, Zijun He, Shuang Xu, Xiangsong Chen, Zhongli Hu, Zhihong Zhang
Ideal Plant Architecture 1 (IPA1) encodes SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 (SPL14) with a pleiotropic effect on regulating rice development and biotic stress responses. To investigate the role of IPA1 in early seedling development, we developed a pair of IPA1/ipal-NILs and found that seed germination and early seedling growth were retarded in the ipa1-NIL. Analysis of the soluble sugar content
...
-
Efficient Production of 3′-Sialyllactose by Single Whole-Cell in One-Pot BiosynthesisProcesses (IF 3.352) Pub Date : 2021-05-26 ,DOI:10.3390/pr9060932Zhongkui Li, Xiangsong Chen, Zhijian Ni, Lixia Yuan, Lijie Sun, Yu Wang, Jinyong Wu, Jianming Yao
Sialyllactose (SL) is one of the most important acidic oligosaccharides in human milk, which plays an important role in the health of infants. In this work, an efficient multi-enzyme cascade was developed in a single whole cell to produce 3′-SL. We constructed two compatible plasmids with double cloning sites to co-express four genes. Different combinations were assessed to verify the optimal catalytic
...
-
Enhanced bioproduction of fucosylated oligosaccharide 3-fucosyllactose in engineered Escherichia coli with an improved de novo pathway.Bioscience, Biotechnology, and Biochemistry (IF 2.337) Pub Date : 2021-04-27 ,DOI:10.1093/bbb/zbab074Zhijian Ni,Jinyong Wu,Zhongkui Li,Lixia Yuan,Yu Wang,Xiangsong Chen,Jianming Yao3-fucosyllactose (3-FL) and 2'-fucosyllactose (2'-FL), are two important fucosylated oligosaccharides in human milk. Extensive studies on 2'-FL enabled its official approval for use in infant formula. However, commercialization of 3-FL seems to be a bit sluggish due to its low content in human milk and poor yield in enlarged production. Here, an α-1,3-fucosyltransferase mutant was expressed in an engineered ...
-
Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coliBiotechnology & Biotechnological Equipment (IF 1.762) Pub Date : 2021-02-25 ,DOI:10.1080/13102818.2021.1885996Yingxue Liao, Zhijian Ni, Jinyong Wu, Zhongkui Li, Yuanfei Ge, Xiangsong Chen, Jianming Yao
Abstract 2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2'-FL production. The highest 2'-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the
...
-
Multi-Path Optimization for Efficient Production of 2′-Fucosyllactose in an Engineered Escherichia coli C41 (DE3) DerivativeFrontiers in Bioengineering and Biotechnology (IF 6.064) Pub Date : 2020-12-03 ,DOI:10.3389/fbioe.2020.611900Zhijian Ni, Zhongkui Li, Jinyong Wu, Yuanfei Ge, Yingxue Liao, Lixia Yuan, Xiangsong Chen, Jianming Yao
2′-fucosyllactose (2′-FL), one of the simplest but most abundant oligosaccharides in human milk, has been demonstrated to have many positive benefits for the healthy development of newborns. However, the high-cost production and limited availability restrict its widespread use in infant nutrition and further research on its potential functions. In this study, on the basis of previous achievements,
...
-
Effect of Sialic Acid on Mammalian Cell Culture and Protein Expression: A Potential Productivity Enhancer for Biopharmaceutical Cell Culture ProcessesProcesses (IF 3.352) Pub Date : 2020-11-12 ,DOI:10.3390/pr8111449Xiangsong Chen, Shang Xiao, Jinyong Wu, Jianming Yao
Improved productivity of the two most commonly used cell lines in the biopharmaceutical industry, such as human embryonic kidney 293 (HEK293) and Chinese hamster ovary (CHO), could reduce production costs and increase manufacturing capacity. One method for increasing protein productivity is the addition of antioxidants during the cell culture process. In this study, we examined the effect of sialic
...
-
Degradation Kinetics and Shelf Life of N-acetylneuraminic Acid at Different pH ValuesMolecules (IF 4.927) Pub Date : 2020-11-05 ,DOI:10.3390/molecules25215141Weiwei Zhu, Xiangsong Chen, Lixia Yuan, Jinyong Wu, Jianming Yao
The objective of this study was to investigate the stability and degradation kinetics of N-acetylneuraminic acid (Neu5Ac). The pH of the solution strongly influenced the stability of Neu5Ac, which was more stable at neutral pH and low temperatures. Here, we provide detailed information on the degradation kinetics of Neu5Ac at different pH values (1.0, 2.0, 11.0 and 12.0) and temperatures (60, 70, 80
...
-
Research progress on the inhibition of enzymes by polyoxometalatesInorganic Chemistry Frontiers (IF 7.779) Pub Date : 2020-09-11 ,DOI:10.1039/d0qi00860eMeijuan Zhao, Xiangsong Chen, Guoxiang Chi, Die Shuai, Li Wang, Bingnian Chen, Jian LiPolyoxometalates (POMs) are a kind of inorganic cluster metal complex with various biological activities, such as anti-Alzheimer's disease, antibacterial, anti-cancer, anti-diabetes, anti-virus, and so on. The mechanism of their action at the molecular level is basically unknown. However, some studies have shown that the inhibitory effect of POMs on several enzyme families (such as α-glucosidase, tyrosinase ...
-
Multi-Enzymatic Cascade One-Pot Biosynthesis of 3′-Sialyllactose Using Engineered Escherichia coliMolecules (IF 4.927) Pub Date : 2020-08-06 ,DOI:10.3390/molecules25163567Zhongkui Li, Zhijian Ni, Xiangsong Chen, Gang Wang, Jinyong Wu, Jianming Yao
Among the human milk oligosaccharides (HMOs), one of the most abundant oligosaccharides and has great benefits for human health is 3′-sialyllactose (3′-SL). Given its important physiological functions and the lack of cost-effective production processes, we constructed an in vitro multi-enzymatic cofactor recycling system for the biosynthesis of 3′-SL from a low-cost substrate. First, we constructed
...
广告：喜“文”乐“荐”|邀请好友使用Taylor & Francis Editing Services编辑润色服务，共享专属优惠！推荐人数无限制！
高级搜索
我的搜索条件
京公网安备 11010802027423号