- 作 者: ( "chengxi cao" OR "cheng-xi cao" )
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Correction: Recent advances in microfluidic-based electroporation techniques for cell membranesLab on a Chip (IF 7.517) Pub Date : 2022-08-02 ,DOI:10.1039/d2lc90068hFei Wang, Shujing Lin, Zixian Yu, Yanpu Wang, Di Zhang, Chengxi Cao, Zhigang Wang, Daxiang Cui, Di Chen
Correction for ‘Recent advances in microfluidic-based electroporation techniques for cell membranes’ by Fei Wang et al., Lab Chip, 2022, 22, 2624–2646, https://doi.org/10.1039/D2LC00122E.
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Recent advances in microfluidic-based electroporation techniques for cell membranesLab on a Chip (IF 7.517) Pub Date : 2022-07-01 ,DOI:10.1039/d2lc00122eFei Wang, Shujing Lin, Zixian Yu, Yanpu Wang, Di Zhang, Chengxi Cao, Zhigang Wang, Daxiang Cui, Di ChenElectroporation is a fundamental technique for applications in biotechnology. To date, the ongoing research on cell membrane electroporation has explored its mechanism, principles and potential applications. Therefore, in this review, we first discuss the primary electroporation mechanism to help establish a clear framework. Within the context of its principles, several critical terms are highlighted ...
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[In-site electrophoretic elution of excessive fluorescein isothiocyanate from fluorescent particles in gel for image analysis].Chinese Journal of Chromatography Pub Date : 2022-07-01 ,DOI:10.3724/sp.j.1123.2022.04023Guohong Chen,Zehua Guo,Yiren Cao,Liuyin Fan,Weiwen Liu,Yixin Ma,Chengxi Cao,Qiang Zhang
The sensitivity, accuracy, and efficiency of fluorescent particle detection can be improved by purifying the fluorescent-dye-labeled particles. In this study, an in-site model of electrophoretic elution (EE) was developed for the facile and efficient removal of unconjugated fluorescent dyes after labeling reactions, thereby facilitating the sensitive fluorescent imaging of proteins captured by microbeads
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[Multi-channel contactless conductivity detection device for online detection of free-flow electrophoresis separation].Se pu = Chinese journal of chromatography Pub Date : 2022-04-01 ,DOI:10.3724/sp.j.1123.2021.11011Ziqi Liang,Qiang Zhang,Xiaoteng Jiang,Xiaoping Liu,Chengxi Cao,Hua Xiao,Weiwen LiuFree-flow electrophoresis (FFE) is an all-liquid-phase electrophoresis technique without any supporting media, which has both analytical and preparative functions. Compared to other electrophoresis techniques, FFE has been used for the separation of peptides, proteins, cells, and microorganisms due to its advantages of mild separation environment, high recovery, and sustainable separation. Both the ...
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A facile online multi-gear capacitively coupled contactless conductivity detector for an automatic and wide range monitoring of high salt in HPLCAnalyst (IF 5.227) Pub Date : 2021-12-27 ,DOI:10.1039/d1an02249kWeiwen Liu, Ziqi Liang, Yuanyu Wang, Jun Cao, Qiang Zhang, Xiaoping Liu, Yuxing Wang, Chengxi CaoSensing the electrolyte solution or aqueous–organic mixture has attracted much interest in chemical separation, pharmaceutical engineering, bioprocess, and biochemical experiments. However, reports on online contactless sensor with automatic and wide range sensing of high content electrolyte have been rarely presented. Herein, a facile model and theory of online multi-gear capacitively coupled contactless ...
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Gel Electrophoresis Chip Using Joule Heat Self-Dissipation, Short Run Time, and Online Dynamic ImagingAnalytical Chemistry (IF 8.008) Pub Date : 2021-12-27 ,DOI:10.1021/acs.analchem.1c03635Jingjing Xue, Qiang Zhang, Jun Cao, Youli Tian, Genhan Zha, Xiaoping Liu, Weiwen Liu, Yuxing Wang, Dingkun Gui, Chengxi CaoGel electrophoresis (GE) is one of the most general tools in biomedicine. However, it suffers from low resolution, and its mechanism has not been fully revealed yet. Herein, we presented the dispersion model of w2 (t) ∝ Tt, showing the band dispersion (w) via temperature (T) and running time (t) control. Second, we designed an efficient GE chip via the time control and rapid Joule heat self-dissipation ...
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Reciprocating free-flow isoelectric focusing with online array ultraviolet detector for process monitoring of protein separationJournal of Chromatography A (IF 4.601) Pub Date : 2021-12-14 ,DOI:10.1016/j.chroma.2021.462747Lin Huang, Qiang Zhang, Youli Tian, Xiaoping Liu, Weiwen Liu, Hua Xiao, Yuxin Wang, Chengxi CaoFree-flow isoelectric focusing (FFIEF) is a useful tool for separating and purifying proteins, DNA, cells, and organelles, etc. However, the online monitoring of each fraction during an FFIEF run has not been achieved yet, resulting in a lack of process monitoring of FFIEF. Herein, an online array ultraviolet (UV) detection system was developed for the easy assay of FFE fractions. The detector was ...
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[Determination of absolute mobility and dissociation constant of lovastatin using capillary electrophoresis and empirical equation of ion mobility].Se pu = Chinese journal of chromatography Pub Date : 2021-12-01 ,DOI:10.3724/sp.j.1123.2021.01014Fang Luo,Zehua Guo,Chengxi Cao,Liuyin Fan,Wei ZhangIn capillary electrophoresis, determination of the basic physical and chemical properties of compounds, such as absolute mobility (m0) and dissociation constant (pKa), is of great practical significance. This is because the aforementioned properties are often used for the qualitative or quantitative analyses of the relevant compounds toward their application as potential drugs. Lovastatin is a potential ...
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Metal Organic Framework Nanomaterial-Based Extraction and Proteome Analysis of Membrane and Membrane-Associated ProteinsAnalytical Chemistry (IF 8.008) Pub Date : 2021-11-24 ,DOI:10.1021/acs.analchem.1c03219Baohui Zhang, Miao Guo, Huiyu Wang, Zeyuan Wang, Lu Zhang, Yan Zhang, Chengxi Cao, Hua XiaoMembrane proteins (MPs) play a key role in various biological processes, while difficulties still exist in the extraction because of their inherent low abundance and poor solubility caused by high hydrophobicity. Metal organic framework (MOF) materials with good hydrophobic properties have the ability to absorb MPs, especially zeolitic imidazolate framework (ZIF) materials. Here, two MOF materials ...
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Immobilized Titanium (IV) Ion Affinity Chromatography Contributes to Efficient Proteomics Analysis of Cellular Nucleic Acid-Binding ProteinsJournal of Proteome Research (IF 5.37) Pub Date : 2021-11-15 ,DOI:10.1021/acs.jproteome.1c00788Huiyu Wang, Liqiang Qian, Zhi Shang, Zeyuan Wang, Yan Zhang, Chengxi Cao, Hua XiaoCellular nucleic acid-binding proteins (NABPs), namely, DNA-binding proteins (DBPs) and RNA-binding proteins (RBPs), play important roles in many biological processes. However, extracting NABPs with high efficiency in living cells is challenging, which greatly limited their proteomics analysis and comprehensive characterization. Here, we discovered that titanium (IV) ion-immobilized metal affinity ...
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[Speculation of hemoglobin A3 peak position in clinical cation exchange high performance liquid chromatogram of the diabetic blood sample with microarray isoelectric focusing].Se pu = Chinese journal of chromatography Pub Date : 2021-11-01 ,DOI:10.3724/sp.j.1123.2020.12033Zehua Guo,Fang Luo,Si Li,Liuyin Fan,Yixin Wu,Chengxi CaoHemoglobin A1c (HbA1c) is a major component of glycated hemoglobin in human red blood cells. It has been proven to be a significant biomarker for the diagnosis of diabetes; its content in fresh red cells in diabetes blood reflects the average level of blood glucose over the previous three months. Thus, HbA1c level has been used for the assessment of long-term glycemic control in diabetes; the level ...
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[Determination of two quaternary ammonium salts in disinfector by portable capillary electrophoresis device based on smartphone].Se pu = Chinese journal of chromatography Pub Date : 2021-11-01 ,DOI:10.3724/sp.j.1123.2021.04030Yuanyu Wang,Ruihua Zhang,Qiang Zhang,Chengxi Cao,Liuyin Fan,Weiwen LiuThe existing miniature capillary electrophoresis (CE) devices use a tablet or a computer for data processing and analysis, which hinders their portability. In order to solve this problem, a smartphone-based CE device was proposed, which allowed for real portable quantitative analysis. The device integrated the functions of capacitively coupled contactless conductivity detection (C4D) and Bluetooth ...
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ExoSD Chips for high-purity immunomagnetic separation and high-sensitivity detection of gastric cancer cell-derived exosomesBiosensors and Bioelectronics (IF 12.545) Pub Date : 2021-08-27 ,DOI:10.1016/j.bios.2021.113594Zixian Yu, Shujing Lin, Fangfang Xia, Yanlei Liu, Di Zhang, Fei Wang, Yanpu Wang, Qichao Li, Jiaqi Niu, Chengxi Cao, Daxiang Cui, Nengquan Sheng, Jiazi Ren, Zhigang Wang, Di ChenGastric cancer cell-derived exosomes as biomarkers have a very high application potential to the non-invasive detection of early-stage gastric cancer. However, the small size of exosomes (30 ∼ 150 nm) results in huge challenges in separating and detecting them from complex media (e.g., plasma, urine, saliva, and cell culture supernatant). Here we proposed a highly integrated exosome separation and ...
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Model, Simulation, and Experiments on Moving Exchange Boundary via Ligand and Quantum Dots in Chip ElectrophoresisAnalytical Chemistry (IF 8.008) Pub Date : 2021-03-23 ,DOI:10.1021/acs.analchem.1c00242Qiang Zhang, Zehua Guo, Fang Luo, Hua Xiao, Weiwen Liu, Liuyin Fan, Chengxi Cao
Herein, the quench model of the moving exchange boundary (MEB) was first created via a ligand of 5,5′-dithiobis(2-nitro-benzoic acid) (DTNB) and group of 3-mercaptopropionic acid (MPA) capped on QDs, and then the recovery model was formed via MPA and 2-nitro-5-thiobenzoic acid (TNB) capped on QDs. The theory on MEB dynamics and width was developed based on the two reversible models, the simulation
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[Identification of the relative age of iron gall ink in handwriting by capillary electrophoresis using the Fe(Ⅱ)/Fe(Ⅲ) ratio].Se pu = Chinese journal of chromatography Pub Date : 2020-10-08 ,DOI:10.3724/sp.j.1123.2020.05033Yu Cai,Chengxi Cao,Xianyi Zhuo,Honggen Li,Liuyin FanIdentifying the relative age of iron gall ink in the handwriting on a questioned file is highly significant for court science, because it serves as important evidence for solving criminal cases and in confirming the authenticity of historical documents. This is because many criminal cases involve analysis of forged documents to conclude whether an entire document is as old as purported, or whether ...
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A facile thermometer-like electrophoresis titration biosensor for alternative miRNA assay via moving reaction boundary chipBiosensors and Bioelectronics (IF 12.545) Pub Date : 2020-10-02 ,DOI:10.1016/j.bios.2020.112676Ling Chen, Qiang Zhang, Weiwen Liu, Hua Xiao, Xiaoping Liu, Liuyin Fan, Yuxing Wang, Honggen Li, Chengxi CaoHerein, a facile thermometer-like model of electrophoresis titration (ET) biosensor was proposed as an alternative tool for miRNA assay via moving reaction boundary (MRB) chip. For proof-of-concept demonstration, miRNA-122 and catalyzed hairpin assembly (CHA) were chosen as the model analyte and amplification, respectively. In the developed ET system, miRNA triggered the CHA with two hairpin probes ...
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Discovery of small extracellular vesicle proteins from human serum for liver cirrhosis and liver cancer.Biochimie (IF 4.372) Pub Date : 2020-08-21 ,DOI:10.1016/j.biochi.2020.08.013Asad Uzzaman,Xuguang Zhang,Zhi Qiao,Hao Zhan,Amir Sohail,Amir Wahid,Zhi Shang,Xin Guan,Cheng-Xi Cao,Hua XiaoHepatocellular carcinoma (HCC) is a common neoplastic transformation of the hepatocytes, which has high morbidity and mortality worldwide, particularly in Eastern Asia. HCC is also developed as a consequence of chronic liver cirrhosis, and both diseases are difficult to diagnosis and differentiate. Accurate noninvasive biomarkers for HCC and cirrhosis are urgently needed. In the search for novel candidates ...
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Free-Flow Isoelectric Focusing for Comprehensive Separation and Analysis of Human Salivary Microbiome for Lung Cancer.Analytical Chemistry (IF 8.008) Pub Date : 2020-08-04 ,DOI:10.1021/acs.analchem.0c02627Xiaoteng Jiang,Sha Liu,Yan Zhang,Yin Ji,Amir Sohail,Chengxi Cao,Peng Wang,Hua XiaoHuman microbiome contains billions of microorganisms that play important roles in the biological system and different diseases. Due to its complexity, conventional culture-independent technology may underestimate the value of low-abundance bacteria, which calls for a highly efficient method for its enrichment and comprehensive analysis. In this study, we developed a recycling free-flow isoelectric ...
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Effects of the Application of a Perforated Anode in an Aluminum Electrolysis Cell on the Gas–Liquid Two-Phase Flow and Bubble Distribution CharacteristicsIndustrial & Engineering Chemistry Research (IF 4.326) Pub Date : 2020-07-22 ,DOI:10.1021/acs.iecr.0c02478Wenyuan Hou, Hesong Li, Yuan Feng, Jiaoru Wang, Mao Li, Benjun Cheng, Xi CaoSchool of Energy Science and Engineering, Central South University, Changsha 410083, ChinaIn contrast to the original form of the anode, the perforated anode is a new kind of anode that can significantly reduce the bubble thickness while maintaining a stable electrolysis process. The bubble movement was simulated within physical and mathematical models of the flow field in the anode–cathode distance (ACD) area, in which the electrolyte solidification zone in the anode perforations was treated ...
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Purification of low-abundance lysozyme in egg white via free-flow electrophoresis with gel-filtration chromatography.Electrophoresis (IF 3.595) Pub Date : 2020-06-24 ,DOI:10.1002/elps.201900479Shuang Dong,Ziqin Jiang,Zhen Liu,Ling Chen,Qiang Zhang,Youli Tian,Amir Sohail,Muhammad Idrees Khan,Hua Xiao,Xiaoping Liu,Yuxing Wang,Honggen Li,Hanyu Wu,Weiwen Liu,Chengxi CaoAs an effective separation tool, free‐flow electrophoresis has not been used for purification of low‐abundance protein in complex sample matrix. Herein, lysozyme in complex egg white matrix was chosen as the model protein for demonstrating the purification of low‐content peptide via an FFE coupled with gel fitration chromatography (GFC). The crude lysozyme in egg while was first separated via free‐flow ...
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[Visualized and quantitative detection of horseradish peroxidase based on redox reaction boundary].Se pu = Chinese journal of chromatography Pub Date : 2020-02-08 ,DOI:10.3724/sp.j.1123.2019.04030Hao Kong,Qiang Zhang,Wei Zhang,Weiwen Liu,Chengxi Cao,Liuyin FanA new portable method for the detection of horseradish peroxidase (HRP) is reported in this paper. Visualized and quantitative detection of HRP was achieved based on the theory of electrophoresis titration (ET) and redox reaction boundary (RB). The ET-RB model was built on the 3,3',5,5'-tetramethyl benzidine chromogenic system because it significantly increases the efficiency of the chromogenic reaction ...
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Glycoprotein fluorescent speed sensing by newly-synthesized boronic complex probe and chip supramolecular electrophoresisSensors and Actuators B: Chemical (IF 9.221) Pub Date : 2020-01-24 ,DOI:10.1016/j.snb.2020.127773Xuejing Wu, Qinghua Meng, Qiang Zhang, Liuyin Fan, Hua Xiao, Chengxi CaoIn this work, we proposed the concept of fluorescent speed sensing (FSS) of glycoprotein in chip supramolecular electrophoresis (CSE). To achieve a stable FSS run, we firstly synthesized a novel boronic acid-based diben-zoxanthene receptor (Rp3) by a simple one-pot reaction and the complex probe of [email protected] containing Rp3 and fluorescein (F1), and performed a series of experiments to assess ...
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Nifuroxazide ameliorates lipid and glucose metabolism in palmitate-induced HepG2 cellsRSC Advances (IF 4.036) Pub Date : 2019-11-29 ,DOI:10.1039/c9ra06527jJing-Yi Liu,Yi-Chen Zhang,Li-Ni Song,Lin Zhang,Fang-Yuan Yang,Xiao-Rong Zhu,Zhi-Qiang Cheng,Xi Cao,Jin-Kui Yang
Inflammation constitutes an important component of non-alcoholic fatty liver disease. STAT3 is a direct target of inflammatory cytokines, but also mediates glycolipid metabolism in the liver. As a potent inhibitor of STAT3, the effect of Nifuroxazide (Nifu) on glycolipid metabolism in liver has not been reported. In this study, we used palmitic acid (PA)-induced HepG2 cells to examine the expression
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FGA isoform as an indicator of targeted therapy for EGFR mutated lung adenocarcinomaJournal of Molecular Medicine (IF 5.606) Pub Date : 2019-11-28 ,DOI:10.1007/s00109-019-01848-zZhi Shang, Xiaomin Niu, Kewei Zhang, Zhi Qiao, Sha Liu, Xiaoteng Jiang, Chengxi Cao, Shun Lu, Hua XiaoEpidermal growth factor receptor (EGFR) gene is frequently mutated in non-small cell lung cancer (NSCLC), which can be targeted by EGFR tyrosine kinase inhibitors (TKIs). It is hard, however, to monitor the performance of EGFR-TKI therapy dynamically. Therefore, therapeutic indicators are urgently needed. Novel antibody microarray, containing 41,472 antibodies, was used for comprehensive analyzing ...
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[Proteomics analysis of serum exosomes and its application in osteoporosis].Chinese Journal of Chromatography Pub Date : 2019-10-24 ,DOI:10.3724/sp.j.1123.2019.04022Chunhui Huo,Yinghua Li,Zhi Qiao,Zhi Shang,Chengxi Cao,Yang Hong,Hua XiaoExosomes are tiny vesicles secreted by cells, can be important mediators of cell-to-cell communication, and play unique roles in disease diagnosis and treatment. Osteoporosis is a metabolic bone disease with high incidence in the elderly, characterized by low bone mineral density and deterioration of bone microstructure. Highly specific diagnostic methods capable of identifying early-stage osteoporosis ...
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Identification of chicken meat quality via rapid array isoelectric focusing with extraction of hemoglobin and myoglobin in meat sample.Journal of Chromatography B (IF 3.318) Pub Date : 2019-09-09 ,DOI:10.1016/j.jchromb.2019.121790Shah Saud,Guoqing Li,Hao Kong,Muhammad Idrees Khan,Zhang Qiang,Yingjie Sun,Weiwen Liu,Chan Ding,Hua Xiao,Yuxing Wang,Honggen Li,Chengxi CaoIsoelectric focusing (IEF) has been used for determination of meat quality with high stability analysis. However, it still suffered from time-consuming, laborious and cost-effective performances, e.g., 3 h protein extraction, more than 10 h rehydration time, 5-12 h focusing time, and imaging of protein band. To overcome these issues, a speedy extraction of colorful proteins was developed by controlling ...
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Facile, Rapid, and Low-Cost Electrophoresis Titration of Thrombin by Aptamer-Linked Magnetic Nanoparticles and a Redox Boundary Chip.ACS Applied Materials & Interfaces (IF 10.383) Pub Date : 2019-08-08 ,DOI:10.1021/acsami.9b09598Hao Kong,Wei-Wen Liu,Wei Zhang,Qiang Zhang,Cun-Huai Wang,Muhammad Idrees Khan,Yu-Xing Wang,Liu-Yin Fan,Cheng-Xi CaoAn aptamer-linked assay of a target biomarker (e.g., thrombin) is facing the challenges of long-term run, complex performance, and expensive instrument, unfitting clinical diagnosis in resource-limited areas. Herein, a facile chip electrophoresis titration (ET) model was proposed for rapid, portable, and low-cost assay of thrombin via aptamer-linked magnetic nanoparticles (MNPs), redox boundary (RB) ...
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Isoelectric focusing array with immobilized pH gradient and dynamic scanning imaging for diabetes diagnosisAnalytica Chimica Acta (IF 6.911) Pub Date : 2019-07-01 ,DOI:10.1016/j.aca.2019.03.014Guo-Qing Li, Hong-Gen Li, Fang-Fang Dong, Yu-Fang Bi, Qiang Zhang, Fan-Zhi Kong, Xiao-Ping Liu, Shah Saud, Hua Xiao, Fang Luo, Ye Peng, Hao-Jie Lu, Liu-Yin Fan, Yu-Xing Wang, Cheng-Xi CaoA traditional immobilized pH gradient (IPG) has a high stability for isoelectric focusing (IEF) but suffers from time-consuming rehydration, focusing and staining-imaging as well as complex performance. To address these issues, an IEF system with an array of 24 IPG columns (10 mm × 600 μm × 50 μm) and dynamic scanning imaging (DSI) was firstly designed for protein focusing. Moreover, two IPG columns ...
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A facile isoelectric focusing of myoglobin and hemoglobin used as markers for screening of chicken meat quality in China.Electrophoresis (IF 3.595) Pub Date : 2019-06-13 ,DOI:10.1002/elps.201900157Shah Saud,Guoqing Li,Yingjie Sun,Muhammad Idrees Khan,Ashfaq Ur Rehman,Asad Uzzaman,Weiwen Liu,Chan Ding,Hua Xiao,Yuxing Wang,Chengxi CaoA novel analytical protocol was developed for general quality screening of chicken meat based on IEF and protein extraction. To demonstrate the developed protocol, 24 chickens were divided into three groups; each had eight chickens. The chickens in Group 1 were slaughtered by exsanguination, Group 2 asphyxiated in water, and that in Group 3 were infected by new castle disease virus. Proteins were extracted ...
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Cancer Cell Derived Small Extracellular Vesicles Contribute to Recipient Cell Metastasis Through Promoting HGF/c-Met Pathway.Molecular & Cellular Proteomics Pub Date : 2019-06-13 ,DOI:10.1074/mcp.ra119.001502Zhi Qiao,Yan Zhang,Maolin Ge,Sha Liu,Xiaoteng Jiang,Zhi Shang,Han Liu,Chengxi Cao,Hua Xiao
Cancer progression is frequently caused by metastasis and leads to significantly increased mortality. Cell derived extracellular vesicles, including exosomes, in the microenvironment play key roles in cellular signal transduction, whereas their biological function in cancer metastasis and progression needs in-depth investigation. Here, we initially demonstrate that the small extracellular vesicles
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