- 作 者: ( "lianrong wang" OR "lian-rong wang" )
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Engineered Phage with Aggregation-Induced Emission Photosensitizer in Cocktail Therapy against Sepsis (Adv. Mater. 6/2023)Advanced Materials (IF 32.086) Pub Date : 2023-02-09 ,DOI:10.1002/adma.202370041Ming-Yu Wu, Luojia Chen, Qingrong Chen, Rui Hu, Xiaoyu Xu, Yifei Wang, Jie Li, Shun Feng, Changjiang Dong, Xiao-Lian Zhang, Zhiqiang Li, Lianrong Wang, Shi Chen, Meijia GuSepsis Therapy ...
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The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria.Microbiology Spectrum Pub Date : 2023-01-04 ,DOI:10.1128/spectrum.03509-22Congrui Xu,Jing Rao,Yuqing Xie,Jiajun Lu,Zhiqiang Li,Changjiang Dong,Lianrong Wang,Jinghong Jiang,Chao Chen,Shi ChenBacterial defense barriers, such as DNA methylation-associated restriction-modification (R-M) and the CRISPR-Cas system, play an important role in bacterial antimicrobial resistance (AMR). Recently, a novel R-M system based on DNA phosphorothioate (PT) modification has been shown to be widespread in the kingdom of Bacteria as well as Archaea. However, the potential role of the PT R-M system in bacterial ...
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The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complexNature Catalysis (IF 40.706) Pub Date : 2022-12-08 ,DOI:10.1038/s41929-022-00884-2Dan Wu, Yaqian Tang, Siwei Chen, Yue He, Xiaofei Chang, Wenzhong Zheng, Zixin Deng, Zhiqiang Li, Lianrong Wang, Geng Wu, Shi ChenBacteria have evolved diverse mechanisms to cope with the constant threat of phage predation. DndABCDE-catalysed DNA phosphorothioate modification, in which the non-bridging oxygen of the DNA sugar-phosphate backbone is replaced by sulfur, can couple with proteins encoded by the dndFGH gene cluster to provide defensive protection, albeit via an obscure mode of action. Here we present structural and ...
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Engineered Phage with Aggregation-Induced Emission Photosensitizer in Cocktail Therapy against SepsisAdvanced Materials (IF 32.086) Pub Date : 2022-11-28 ,DOI:10.1002/adma.202208578Ming-Yu Wu, Luojia Chen, Qingrong Chen, Rui Hu, Xiaoyu Xu, Yifei Wang, Jie Li, Shun Feng, Changjiang Dong, Xiao-Lian Zhang, Zhiqiang Li, Lianrong Wang, Shi Chen, Meijia GuSepsis, a widely recognized disease, is characterized by multiple pathogen infections. Therefore, it is imperative to develop methods that can efficiently identify and neutralize pathogen species. Phage cocktail therapy utilizes the host specificity of phages to adapt to infect resistant bacteria. However, its low sterilization stability efficiency and lack of imaging units seriously restrict its application ...
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Rapid quantification of phenobarbital and barbital in human whole blood by liquid–liquid extraction combined with DART-orbitrap-HRMSForensic Toxicology (IF 2.541) Pub Date : 2022-11-27 ,DOI:10.1007/s11419-022-00650-wShi Ke, Ru Lian, Rong Wang, Yulan Rao, Chen Liang, Jianying Liang, Yurong ZhangPurpose This study aims to develop and validate a rapid, simple, and efficient bioanalytical method for the simultaneous quantification of phenobarbital and barbital in human whole blood using liquid–liquid extraction combined with direct analysis in real time (DART) and high-resolution mass spectrometry (HRMS). Method Phenobarbital-d5 and aprobarbital were selected as internal standards (ISs) of phenobarbital ...
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Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspENature Communications (IF 17.694) Pub Date : 2022-11-09 ,DOI:10.1038/s41467-022-34505-0Haiyan Gao, Xinqi Gong, Jinchuan Zhou, Yubing Zhang, Jinsong Duan, Yue Wei, Liuqing Chen, Zixin Deng, Jiawei Wang, Shi Chen, Geng Wu, Lianrong Wang
DNA phosphorothioate (PT) modification, with a nonbridging phosphate oxygen substituted by sulfur, represents a widespread epigenetic marker in prokaryotes and provides protection against genetic parasites. In the PT-based defense system Ssp, SspABCD confers a single-stranded PT modification of host DNA in the 5′-CPSCA-3′ motif and SspE impedes phage propagation. SspE relies on PT modification in host
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C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2αTheranostics (IF 11.6) Pub Date : 2022-10-17 ,DOI:10.7150/thno.76138citeWenzhong Zheng, Kexin Wang, Yachen Wu, Ge Yan, Chi Zhang, Zhiqiang Li, Lianrong Wang, Shi ChenRationale: A C9orf72 hexanucleotide repeat expansion (GGGGCC) is the most common genetic origin of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Haploinsufficiency of C9orf72 has been proposed as a possible disease mechanism (loss-of-function mechanism). Additionally, the aberrantly activated unfolded protein response (UPR) and stress granule (SG) formation are associated with ...
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Systematic strategies for developing phage resistant Escherichia coli strainsNature Communications (IF 17.694) Pub Date : 2022-08-02 ,DOI:10.1038/s41467-022-31934-9Xuan Zou, Xiaohong Xiao, Ziran Mo, Yashi Ge, Xing Jiang, Ruolin Huang, Mengxue Li, Zixin Deng, Shi Chen, Lianrong Wang, Sang Yup Lee
Phages are regarded as powerful antagonists of bacteria, especially in industrial fermentation processes involving bacteria. While bacteria have developed various defense mechanisms, most of which are effective against a narrow range of phages and consequently exert limited protection from phage infection. Here, we report a strategy for developing phage-resistant Escherichia coli strains through the
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Involvement of the DNA Phosphorothioation System in TorR Binding and Anaerobic TMAO Respiration in Salmonella entericamBio (IF 7.786) Pub Date : 2022-04-14 ,DOI:10.1128/mbio.00699-22You Tang, Yue Hong, Li Liu, Xuan Du, Yaqi Ren, Susu Jiang, Wanjing Liu, Chen Chao, Zixin Deng, Lianrong Wang, Shi Chen aMinistry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal Disease, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan UniversitygridmBio, Ahead of Print. ...
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Structural and Functional Analysis of DndE Involved in DNA Phosphorothioation in the Haloalkaliphilic Archaea Natronorubrum bangense JCM10635mBio (IF 7.786) Pub Date : 2022-04-14 ,DOI:10.1128/mbio.00716-22Wei He, Haiyan Gao, Dan Wu, Susu Jiang, Wanqiu Huang, Chao Chen, Zixin Deng, Lei Xiong, Geng Wu, Lianrong Wang aMinistry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal Disease, Department of Gastroenterology, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan UniversitygridmBio, Ahead of Print. ...
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The origin and impeded dissemination of the DNA phosphorothioation system in prokaryotesNature Communications (IF 17.694) Pub Date : 2021-11-04 ,DOI:10.1038/s41467-021-26636-7Huahua Jian, Guanpeng Xu, Yi Yi, Yali Hao, Yinzhao Wang, Lei Xiong, Siyuan Wang, Shunzhang Liu, Canxing Meng, Jiahua Wang, Yue Zhang, Chao Chen, Xiaoyuan Feng, Haiwei Luo, Hao Zhang, Xingguo Zhang, Lianrong Wang, Zhijun Wang, Zixin Deng, Xiang Xiao
Phosphorothioate (PT) modification by the dnd gene cluster is the first identified DNA backbone modification and constitute an epigenetic system with multiple functions, including antioxidant ability, restriction modification, and virus resistance. Despite these advantages for hosting dnd systems, they are surprisingly distributed sporadically among contemporary prokaryotic genomes. To address this
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Photosensitizers: A Membrane-Targeting Photosensitizer with Aggregation-Induced Emission Characteristics for Highly Efficient Photodynamic Combat of Human Coronaviruses (Small 30/2021)Small (IF 15.153) Pub Date : 2021-07-28 ,DOI:10.1002/smll.202170158Ming-Yu Wu, Meijia Gu, Jong-Kai Leung, Xinmei Li, Yuncong Yuan, Chao Shen, Lianrong Wang, Engui Zhao, Sijie ChenLianrong Wang, Engui Zhao, Sijie Chen and co-workers (article number 2101770) develop a novel membrane-targeting photosensitizer (DTTPB) with aggregation-induced emission characteristics for efficient photodynamic inactivation of human coronaviruses. DTTPB can bind to the envelope of human coronaviruses and sensitize the production of reactive oxygen species, which can effectively inactivate human ...
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A Membrane-Targeting Photosensitizer with Aggregation-Induced Emission Characteristics for Highly Efficient Photodynamic Combat of Human CoronavirusesSmall (IF 15.153) Pub Date : 2021-06-30 ,DOI:10.1002/smll.202101770Ming-Yu Wu, Meijia Gu, Jong-Kai Leung, Xinmei Li, Yuncong Yuan, Chao Shen, Lianrong Wang, Engui Zhao, Sijie Chen
COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, has resulted in global social and economic disruption, putting the world economy to the largest global recession since the Great Depression. To control the spread of COVID-19, cutting off the transmission route is a critical step. In this work, the efficient inactivation of human coronavirus with photodynamic therapy (PDT)
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SspABCD-SspFGH Constitutes a New Type of DNA Phosphorothioate-Based Bacterial Defense SystemmBio (IF 7.786) Pub Date : 2021-04-27 ,DOI:10.1128/mbio.00613-21Shiwei Wang, Mengping Wan, Ruolin Huang, Yujing Zhang, Yuqing Xie, Yue Wei, Mustafa Ahmad, Dan Wu, Yue Hong, Zixin Deng, Shi Chen, Zhiqiang Li, Lianrong Wang, Paul Keim
Unlike nucleobase modifications in canonical restriction-modification systems, DNA phosphorothioate (PT) epigenetic modification occurs in the DNA sugar-phosphate backbone when the nonbridging oxygen is replaced by sulfur in a double-stranded (ds) or single-stranded (ss) manner governed by DndABCDE or SspABCD, respectively. SspABCD coupled with SspE constitutes a defense barrier in which SspE depends
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Human-pathogenic relapsing fever Borrelia found in bats from Central China phylogenetically clustered together with relapsing fever borreliae reported in the New World.PLOS Neglected Tropical Diseases (IF 4.781) Pub Date : 2021-03-18 ,DOI:10.1371/journal.pntd.0009113Ze-Min Li,Xiao Xiao,Chuan-Min Zhou,Jian-Xiao Liu,Xiao-Lan Gu,Li-Zhu Fang,Bin-Yan Liu,Lian-Rong Wang,Xue-Jie Yu,Hui-Ju Han
Bats can harbor zoonotic pathogens causing emerging infectious diseases, but their status as hosts for bacteria is limited. We aimed to investigate the distribution, prevalence and genetic diversity of Borrelia in bats and bat ticks in Hubei Province, China, which will give us a better understanding of the risk of Borrelia infection posed by bats and their ticks. During 2018-2020, 403 bats were captured
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Single-molecule optical mapping of the distribution of DNA phosphorothioate epigeneticsNucleic Acids Research (IF 19.16) Pub Date : 2021-03-02 ,DOI:10.1093/nar/gkab169Yue Wei, Qinqin Huang, Xihao Tian, Mingmin Zhang, Junkai He, Xingxiang Chen, Chao Chen, Zixin Deng, Zhiqiang Li, Shi Chen, Lianrong Wang
DNA phosphorothioate (PT) modifications, with the nonbridging phosphate oxygen replaced by sulfur, governed by DndABCDE or SspABCD, are widely distributed in prokaryotes and have a highly unusual feature of occupying only a small portion of available consensus sequences in a genome. Despite the presence of plentiful non-PT-protected consensuses, DNA PT modification is still employed as a recognition
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Oxidative stress-induced mitophagy is suppressed by the miR-106b-93-25 cluster in a protective mannerCell Death & Disease (IF 9.685) Pub Date : 2021-02-24 ,DOI:10.1038/s41419-021-03484-3Cheng Zhang, Pengqing Nie, Chunliu Zhou, Yue Hu, Suling Duan, Meijia Gu, Dongxu Jiang, Yunfu Wang, Zixin Deng, Jincao Chen, Shi Chen, Lianrong Wang
Increased reactive oxygen species levels in the mitochondrial matrix can induce Parkin-dependent mitophagy, which selectively degrades dysfunctional mitochondria via the autolysosome pathway. Phosphorylated mitofusin-2 (MFN2), a receptor of parkin RBR E3 ubiquitin-protein ligase (Parkin), interacts with Parkin to promote the ubiquitination of mitochondrial proteins; meanwhile, the mitophagy receptors
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1Autophagy (IF 13.391) Pub Date : 2021-02-08 ,DOI:10.1080/15548627.2020.1797280Cun-Yu Wang, Dong Wang, Fangyang Wang, Feng Wang, Fengming Wang, Guansong Wang, Han Wang, Hao Wang, Hexiang Wang, Hong-Gang Wang, Jianrong Wang, Jigang Wang, Jiou Wang, Jundong Wang, Kui Wang, Lianrong Wang, Liming Wang, Maggie Haitian Wang, Meiqing Wang, Nanbu Wang, Pengwei Wang, Peipei Wang, Ping Wang, Ping Wang, Qing Jun Wang, Qing Wang, Qing Kenneth Wang, Qiong A. Wang, Wen-Tao Wang, Wuyang Wang
ABSTRACT In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)Autophagy (IF 13.391) Pub Date : 2021-02-08 ,DOI:Cun-Yu Wang, Dong Wang, Fangyang Wang, Feng Wang, Fengming Wang, Guansong Wang, Han Wang, Hao Wang, Hexiang Wang, Hong-Gang Wang, Jianrong Wang, Jigang Wang, Jiou Wang, Jundong Wang, Kui Wang, Lianrong Wang, Liming Wang, Maggie Haitian Wang, Meiqing Wang, Nanbu Wang, Pengwei Wang, Peipei Wang, Ping Wang, Ping Wang, Qing Jun Wang, Qing Wang, Qing Kenneth Wang, Qiong A. Wang, Wen-Tao Wang, Wuyang WangABSTRACT In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite ...
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Measuring maternal body composition by biomedical impedance can predict risk for gestational diabetes mellitus: a retrospective study among 22,223 womenThe Journal of Maternal-Fetal & Neonatal Medicine (IF 2.323) Pub Date : 2020-07-28 ,DOI:10.1080/14767058.2020.1797666Rui-Yuan Zhang, Lan Wang, Wei Zhou, Qi-Mei Zhong, Chao Tong, Ting Zhang, Ting-Li Han, Lian-Rong Wang, Xin Fan, Yan Zhao, Rui-Tu Ran, Yin-Yin Xia, Hong-Bo Qi, Hua Zhang, Tom Norris, Philip N. Baker, Richard SafferyAbstract Objectives This study aimed to identify which element of body composition measurements taken before 17th week gestation was the strongest risk factor for gestational diabetes mellitus (GDM) in Chinese pregnant women. Design and setting A retrospective study was performed using data retrieved from the Electronic Medical Record database of Chongqing Health Center for Women and Children (China) ...
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Epigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria.Proceedings of the National Academy of Sciences of the United States of America (IF 12.779) Pub Date : 2020-06-23 ,DOI:10.1073/pnas.2002933117Xiaolin Wu,Bo Cao,Patricia Aquino,Tsu-Pei Chiu,Chao Chen,Susu Jiang,Zixin Deng,Shi Chen,Remo Rohs,Lianrong Wang,James E Galagan,Peter C DedonPhosphorothioate (PT) DNA modifications—in which a nonbonding phosphate oxygen is replaced with sulfur—represent a widespread, horizontally transferred epigenetic system in prokaryotes and have a highly unusual property of occupying only a small fraction of available consensus sequences in a genome. Using Salmonella enterica as a model, we asked a question of fundamental importance: How do the PT-modifying ...
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Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage.Nucleic Acids Research (IF 19.16) Pub Date : 2020-06-02 ,DOI:10.1093/nar/gkaa473Bo Cao,Xiaolin Wu,Jieliang Zhou,Hang Wu,Lili Liu,Qinghua Zhang,Michael S DeMott,Chen Gu,Lianrong Wang,Delin You,Peter C Dedon
DNA damage and epigenetic marks are well established to have profound influences on genome stability and cell phenotype, yet there are few technologies to obtain high-resolution genomic maps of the many types of chemical modifications of DNA. Here we present Nick-seq for quantitative, sensitive, and accurate mapping of DNA modifications at single-nucleotide resolution across genomes. Pre-existing breaks
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Advances in drug development for targeted therapies for glioblastoma.Medicinal Research Reviews (IF 12.388) Pub Date : 2020-05-23 ,DOI:10.1002/med.21676Ge Yan,Yunfu Wang,Jincao Chen,Wenzhong Zheng,Changzhen Liu,Shi Chen,Lianrong Wang,Jie Luo,Zhiqiang LiGlioblastoma is the most aggressive primary brain tumor in adults. The prognosis of patients with primary glioblastoma treated with the current standard of care, tumor resection followed by radiation therapy and auxiliary temozolomide, remains poor. Integrative genomic analyses have identified essential core signaling pathways and frequent genetic aberrations, which provide potential drug targets for ...
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Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System.mBio (IF 7.786) Pub Date : 2020-04-28 ,DOI:10.1128/mbio.00488-20Liqiong Liu,Susu Jiang,Mai Xing,Chao Chen,Chongde Lai,Na Li,Guangfeng Liu,Dan Wu,Haiyan Gao,Liang Hong,Pan Tan,Shi Chen,Zixin Deng,Geng Wu,Lianrong Wang
DNA phosphorothioate (PT) modification, in which the nonbridging oxygen in the sugar-phosphate backbone is substituted by sulfur, is catalyzed by DndABCDE or SspABCD in a double-stranded or single-stranded manner, respectively. In Dnd and Ssp systems, mobilization of sulfur in PT formation starts with the activation of the sulfur atom of cysteine catalyzed by the DndA and SspA cysteine desulfurases
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SspABCD-SspE is a phosphorothioation-sensing bacterial defence system with broad anti-phage activities.Nature Microbiology (IF 30.964) Pub Date : 2020-04-06 ,DOI:10.1038/s41564-020-0700-6Yubing Zhang,Rui Su,Xianyue Jiang,Mengxue Li,Haiyan Gao,Xihao Tian,Yizhou Zhang,Li Hu,Si Chen,You Tang,Susu Jiang,Ruolin Huang,Zhiqiang Li,Yunfu Wang,Zixin Deng,Jiawei Wang,Peter C Dedon,Shi Chen,Lianrong WangBacteria have evolved diverse mechanisms to fend off predation by bacteriophages. We previously identified the Dnd system, which uses DndABCDE to insert sulfur into the DNA backbone as a double-stranded phosphorothioate (PT) modification, and DndFGH, a restriction component. Here, we describe an unusual SspABCD–SspE PT system in Vibrio cyclitrophicus, Escherichia coli and Streptomyces yokosukanensis ...
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The Biological Applications of Two Aggregation-Induced Emission Luminogens.Biotechnology Journal (IF 5.726) Pub Date : 2019-09-17 ,DOI:10.1002/biot.201900212Meijia Gu,Zixuan Zeng,Mai Xing,Yige Xiong,Zixin Deng,Shi Chen,Lianrong WangFluorescence imaging, as a commonly used scientific tool, is widely applied in various biomedical and material structures through visualization technology. Highly selective and sensitive luminescent biological probes, as well as those with good water solubility, are urgently needed for biomedical research. In contrast to the traditional aggregation-caused quenching of fluorescence, in the unique phenomenon ...
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Gut microbiome interventions in human health and diseases.Medicinal Research Reviews (IF 12.388) Pub Date : 2019-04-17 ,DOI:10.1002/med.21584Pengqing Nie,Zhiqiang Li,Yimeng Wang,Yubing Zhang,Mengna Zhao,Jie Luo,Shiming Du,Zixin Deng,Jincao Chen,Yunfu Wang,Shi Chen,Lianrong WangOngoing studies have determined that the gut microbiota is a major factor influencing both health and disease. Host genetic factors and environmental factors contribute to differences in gut microbiota composition and function. Intestinal dysbiosis is a cause or a contributory cause for diseases in multiple body systems, ranging from the digestive system to the immune, cardiovascular, respiratory, ...
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A new type of DNA phosphorothioation-based antiviral system in archaeaNature Communications (IF 17.694) Pub Date : 2019-04-11 ,DOI:10.1038/s41467-019-09390-9Lei Xiong,Siyi Liu,Si Chen,Yao Xiao,Bochen Zhu,Yali Gao,Yujing Zhang,Beibei Chen,Jie Luo,Zixin Deng,Xiangdong Chen,Lianrong Wang,Shi Chen
Archaea and Bacteria have evolved different defence strategies that target virtually all steps of the viral life cycle. The diversified virion morphotypes and genome contents of archaeal viruses result in a highly complex array of archaea-virus interactions. However, our understanding of archaeal antiviral activities lags far behind our knowledges of those in bacteria. Here we report a new archaeal
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Advances in CRISPR-Cas systems for RNA targeting, tracking and editing.Biotechnology Advances (IF 17.681) Pub Date : 2019-03-27 ,DOI:10.1016/j.biotechadv.2019.03.016Fei Wang,Lianrong Wang,Xuan Zou,Suling Duan,Zhiqiang Li,Zixin Deng,Jie Luo,Sang Yup Lee,Shi ChenClustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, especially type II (Cas9) systems, have been widely used in gene/genome targeting. Modifications of Cas9 enable these systems to become platforms for precise DNA manipulations. However, the utilization of CRISPR-Cas systems in RNA targeting remains preliminary. The discovery of type VI CRISPR-Cas ...
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Tight control of genomic phosphorothioate modification by the ATP-modulated autoregulation and reusability of DndB.Molecular Microbiology (IF 3.979) Pub Date : 2019-03-18 ,DOI:10.1111/mmi.14186Sisi Xia,Jun Chen,Liqiong Liu,Yue Wei,Zixin Deng,Lianrong Wang,Shi Chen
DNA phosphorothioate (PT) modification was recently identified to occur naturally in diverse bacteria and to be governed by DndABCDE proteins. The nuclease resistance as well as the redox and nucleophilic properties of PT sulfur make PT modification a versatile player in restriction-modification (R-M) defense, epigenetic regulation, environmental fitness and the maintenance of cellular redox homeostasis
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