- 作 者: ( "zishu pan" OR "zi-shu pan" )
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Construction and efficacy of a new live chimeric C-strain vaccine with DIVA characteristics against classical swine feverVaccine (IF 4.169) Pub Date : 2023-02-18 ,DOI:10.1016/j.vaccine.2023.02.044Weicheng Yi, Hao Wang, Huan Qin, Qin Wang, Rui Guo, Guoyuan Wen, Zishu PanTo develop the new classical swine fever (CSF) vaccine candidate with differentiating infected vaccinated animals (DIVA) characteristics, a chimeric CSF virus (CSFV) was constructed based on an infectious cDNA clone of the CSF vaccine C-strain. The 5’- and 3’-untranslated regions (UTRs) and partial E2 region (residues 690-860) of the C-strain were substituted with the corresponding regions of bovine ...
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Virus-like particles containing a prefusion-stabilized F protein induce a balanced immune response and confer protection against respiratory syncytial virus infection in miceFrontiers in Immunology (IF 8.786) Pub Date : 2022-12-12 ,DOI:10.3389/fimmu.2022.1054005Jin Luo, Huan Qin, Lei Lei, Wange Lou, Ruitong Li, Zishu Pan
Respiratory syncytial virus (RSV) is a serious respiratory pathogen in infants and young children worldwide. Currently, no licensed RSV vaccines are available. In this study, we explored stable prefusion conformation virus-like particles (Pre-F VLPs) as RSV vaccine candidates. RSV fusion (F) protein mutants were constructed to form stabilized Pre-F or postfusion (Post-F) configurations. VLPs containing
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Role of the conserved E2 residue G259 in classical swine fever virus production and replicationVirus Research (IF 6.286) Pub Date : 2022-03-18 ,DOI:10.1016/j.virusres.2022.198747Weicheng Yi, Fengwei Zheng, Hongchang Zhu, Yihan Wu, Jiayang Wei, Zishu PanThe E2 glycoprotein of classical swine fever virus (CSFV) plays multiple roles in the viral life cycle. The chimeric live attenuated C strain with the E2 substitution of bovine viral diarrhea virus (BVDV) is a promising marker vaccine candidate. In this study, the recombinant chimeric CSFV/bE2 cDNA clone harboring heterologous E2 (bE2) of BVDV was constructed by genetic approaches. Recombinant infectious ...
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Data-driven multi-scale mathematical modeling of SARS-CoV-2 infection reveals heterogeneity among COVID-19 patients.PLOS Computational Biology (IF 4.779) Pub Date : 2021-11-24 ,DOI:10.1371/journal.pcbi.1009587Shun Wang,Mengqian Hao,Zishu Pan,Jinzhi Lei,Xiufen Zou
Patients with coronavirus disease 2019 (COVID-19) often exhibit diverse disease progressions associated with various infectious ability, symptoms, and clinical treatments. To systematically and thoroughly understand the heterogeneous progression of COVID-19, we developed a multi-scale computational model to quantitatively understand the heterogeneous progression of COVID-19 patients infected with severe
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Proline to Threonine Mutation at Position 162 of NS5B of Classical Swine Fever Virus Vaccine C Strain Promoted Genome Replication and Infectious Virus Production by Facilitating Initiation of RNA SynthesisViruses (IF 5.818) Pub Date : 2021-08-02 ,DOI:10.3390/v13081523Huining Pang, Ling Li, Hongru Liu, Zishu Pan
The 3′untranslated region (3′UTR) and NS5B of classical swine fever virus (CSFV) play vital roles in viral genome replication. In this study, two chimeric viruses, vC/SM3′UTR and vC/b3′UTR, with 3′UTR substitution of CSFV Shimen strain or bovine viral diarrhea virus (BVDV) NADL strain, were constructed based on the infectious cDNA clone of CSFV vaccine C strain, respectively. After virus rescue, each
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Correction to: Dynamic Host Immune and Transcriptomic Responses to Respiratory Syncytial Virus Infection in a Vaccination-Challenge Mouse ModelVirologica Sinica (IF 6.947) Pub Date : 2021-07-13 ,DOI:10.1007/s12250-021-00424-5Yu Zhao, Chen Ma, Jie Yang, Xiufen Zou, Zishu Pan
A correction to this paper has been published: https://doi.org/10.1007/s12250-021-00418-3
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Dynamic Host Immune and Transcriptomic Responses to Respiratory Syncytial Virus Infection in a Vaccination-Challenge Mouse ModelVirologica Sinica (IF 6.947) Pub Date : 2021-06-17 ,DOI:10.1007/s12250-021-00418-3Yu Zhao, Chen Ma, Jie Yang, Xiufen Zou, Zishu PanRespiratory syncytial virus (RSV) is the major cause of lower respiratory tract infections in children. Inactivated RSV vaccine was developed in the late 1960’s, but the vaccine-enhanced disease (VED) occurred to vaccinated infants upon subsequent natural RSV infection. The excessive inflammatory immunopathology in the lungs might be involved in the VED, but the underlying mechanisms remain not fully ...
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Chimeric enterovirus 71 virus-like particle displaying conserved coxsackievirus A16 epitopes elicits potent immune responses and protects mice against lethal EV71 and CA16 infectionVaccine (IF 4.169) Pub Date : 2021-06-09 ,DOI:10.1016/j.vaccine.2021.05.093Jin Luo, Chunling Huo, Huan Qin, Junhong Hu, Lei Lei, Zishu PanHand-foot-and-mouth disease (HFMD) is an infectious disease of infants and young children frequently caused by the enterovirus A species, mainly enterovirus 71 (EV71) and coxsackievirus A16 (CA16). In this study, we prepared the EV71 virus-like particle (EV71-VLP) and its chimeras using recombinant baculovirus (Bac-P1-3CD) co-expressing EV71 P1 (under polyhedrin promoter) and 3CD (under CMV-IE promoter) ...
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Construction and immunological evaluation of hepatitis B virus core virus-like particles containing multiple antigenic peptides of respiratory syncytial virusVirus Research (IF 6.286) Pub Date : 2021-04-02 ,DOI:10.1016/j.virusres.2021.198410Lei Lei, Huan Qin, Jin Luo, Yiluo Tan, Jie Yang, Zishu PanRespiratory syncytial virus (RSV) infection causes severe disease in the lower respiratory tract of infants and young children. Currently, no licensed vaccine is available. In this study, we generated the chimeric virus-like particles (tHBc/FE1E2, tHBc/FE1E2/M282-90 and tHBc/FE1E2/M282-90/tG VLPs) containing multiple antigenic peptides of RSV proteins based on a truncated hepatitis B virus core carrier ...
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A positively charged surface patch on the pestivirus NS3 protease module plays an important role in modulating NS3 helicase activity and virus production.Archives of Virology (IF 2.685) Pub Date : 2021-03-31 ,DOI:10.1007/s00705-021-05055-5Fengwei Zheng,Weicheng Yi,Weichi Liu,Hongchang Zhu,Peng Gong,Zishu Pan
Pestivirus nonstructural protein 3 (NS3) is a multifunctional protein with protease and helicase activities that are essential for virus replication. In this study, we used a combination of biochemical and genetic approaches to investigate the relationship between a positively charged patch on the protease module and NS3 function. The surface patch is composed of four basic residues, R50, K74 and K94
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(p)ppGpp synthetases are required for the pathogenicity of Salmonella Pullorum in chickensMicrobiological Research (IF 5.07) Pub Date : 2021-01-05 ,DOI:10.1016/j.micres.2020.126685Xin Wang, Yiluo Cheng, Wenting Zhang, Qin Lu, Guoyuan Wen, Qingping Luo, Huabin Shao, Zishu Pan, Tengfei ZhangSalmonella Pullorum is a pathogen specific to birds that can cause Pullorum disease in young chickens and lead to considerable economic losses in the poultry industry. During transmission and infection, S. Pullorum will encounter various environmental stresses and host defenses. The stringent response is an important adaptation response induced by (p)ppGpp, and in Salmonella, (p)ppGpp is synthesized ...
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Characterization of Salmonella spp. isolated from chickens in Central China.BMC Veterinary Research (IF 2.792) Pub Date : 2020-08-20 ,DOI:10.1186/s12917-020-02513-1Xin Wang,Honglin Wang,Tingting Li,Feifei Liu,Yiluo Cheng,Xiaodong Guo,Guoyuan Wen,Qingping Luo,Huabin Shao,Zishu Pan,Tengfei Zhang
Salmonella is an important zoonotic pathogen, and chickens are one of its main hosts. Every year, Salmonella infections pose a serious threat to the poultry industry in developing countries, especially China. In this study, a total of 84 Salmonella isolates recovered from sick and healthy-looking chickens in central China were characterized by serotyping, MLST-based strain typing, presence of potential
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Hepatitis B Virus Core Particles Containing a Conserved Region of the G Protein Combined with Interleukin-35 Protected Mice against Respiratory Syncytial Virus Infection without Vaccine-Enhanced Immunopathology.Journal of Virology (IF 6.549) Pub Date : 2020-06-16 ,DOI:10.1128/jvi.00007-20Jie Yang,Chen Ma,Yu Zhao,Anjing Fan,Xiufen Zou,Zishu PanRespiratory syncytial virus (RSV) is the most important cause of lower respiratory tract infection in infants and young children. The vaccine-enhanced disease (VED) has greatly hindered the development of an RSV vaccine. Currently, there are no licensed vaccines for RSV. In this study, immunization of mice with hepatitis B virus core particles containing a conserved region of the G protein (HBc-tG) ...
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Y-Box-Binding Protein 3 (YBX3) Restricts Influenza A Virus by Interacting with Viral Ribonucleoprotein Complex and Imparing its Function.Journal of General Virology (IF 5.141) Pub Date : 2020-04-01 ,DOI:10.1099/jgv.0.001390Zhenqiao Qin,Xiao Qu,Lei Lei,Lulai Xu,Zishu PanThe influenza A virus (IAV) ribonucleoprotein (vRNP) complex consists of polymerase subunits, nucleoprotein (NP) and viral RNA and is responsible for RNA transcription and replication. Interactions between the vRNP complex and host factors play important roles in virus replication, pathogenicity and species tropism. In this study, Strep-tag affinity purification coupled with mass spectrometry was used ...
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Characterization of thermostable Newcastle disease virus recombinants expressing the hemagglutinin of H5N1 avian influenza virus as bivalent vaccine candidates.Vaccine (IF 4.169) Pub Date : 2020-01-11 ,DOI:10.1016/j.vaccine.2019.12.054Lulai Xu,Zhenqiao Qin,Lei Qiao,Jie Wen,Huabin Shao,Guoyuan Wen,Zishu PanNewcastle disease virus (NDV) has been used as a vector in the development of vaccines and gene delivery. In the present study, we generated the thermostable recombinant NDV (rNDV) expressing the different forms of hemagglutinin (HA) of highly pathogenic avian influenza virus (HPAIV) H5N1 based on the full-length cDNA clone of thermostable TS09-C strain. The recombinant thermostable Newcastle disease ...
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Japanese Encephalitis Virus Induces Apoptosis and Encephalitis by Activating the PERK Pathway.Journal of Virology (IF 6.549) Pub Date : 2019-08-13 ,DOI:10.1128/jvi.00887-19Qianruo Wang,Xiu Xin,Ting Wang,Jiawu Wan,Yangtao Ou,Zibing Yang,Qijia Yu,Liting Zhu,Yunli Guo,Yinsheng Wu,Zhen Ding,Yanni Zhang,Zishu Pan,Yuxin Tang,Shanshan Li,Lingbao Kong
Accumulated evidence demonstrates that Japanese encephalitis virus (JEV) infection triggers endoplasmic reticulum (ER) stress and neuron apoptosis. ER stress sensor protein kinase R-like endoplasmic reticulum kinase (PERK) has been reported to induce apoptosis under acute or prolonged ER stress. However, the precise role of PERK in JEV-induced apoptosis and encephalitis remains unknown. Here, we report
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A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine.Journal of Virological Methods (IF 2.623) Pub Date : 2019-04-02 ,DOI:10.1016/j.jviromet.2019.04.001Yan Zhao,Feifei Liu,Qingmei Li,Mengfan Wu,Lei Lei,Zishu Pan
A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis coronavirus (TGEV) were selected based on alignments
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Japanese encephalitis virus induces apoptosis by inhibiting Foxo signaling pathway.Veterinary Microbiology (IF 3.246) Pub Date : 2018-06-11 ,DOI:10.1016/j.vetmic.2018.05.008Fenglin Guo,Xilan Yu,Ahui Xu,Jing Xu,Qianruo Wang,Yunli Guo,Xiaoyu Wu,Yuxin Tang,Zhen Ding,Yanni Zhang,Tian Gong,Zishu Pan,Shanshan Li,Lingbao KongJapanese encephalitis virus (JEV) infection induces brain tissue disease characterized by neuron death. however, little is known about the underlying mechanism. Using RNA sequencing, we profiled global mRNA expression changes in response to in vitro and in vivo JEV infection. Integration analysis of in vitro and in vivo mRNA transcriptome revealed that JEV infection regulated apoptosis-related Foxo ...
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Hepatitis B virus core particles containing multiple epitopes confer protection against enterovirus 71 and coxsackievirus A16 infection in mice.Vaccine (IF 4.169) Pub Date : 2017-11-14 ,DOI:10.1016/j.vaccine.2017.10.101Chunling Huo,Jie Yang,Lei Lei,Lei Qiao,Jiantao Xin,Zishu PanHuman enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are the two major causative agents of hand-foot-and-mouth disease (HFMD). To investigate novel combined vaccines to prevent EV71 and CA16 infection, we constructed chimeric virus-like particles (tHBc/SPA or tHBc/SP VLPs) displaying conserved epitopes of EV71 (aa 208-222 of VP1 and aa 248-263 of VP2) and CA16 (aa271-285 of VP1) using a truncated ...
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Classical swine fever virus nonstructural protein p7 modulates infectious virus production.Scientific Reports (IF 4.996) Pub Date : 2017-Oct-1 ,DOI:10.1038/s41598-017-13352-wCheng Zhao,Xiaofang Shen,Rui Wu,Ling Li,Zishu Pan
The classical swine fever virus (CSFV) nonstructural protein p7 is crucial for virus production, yet precisely how the p7 modulates this process is unclear. In this study, we first identified the interactions of p7 with E2 and NS2. The key binding regions of both p7 and NS2 mapped to the first transmembrane (TM1) domain of two proteins. Three amino acid substitutions in the TM1 region of p7 (p7TDI18/19/20AAA
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Uncoupling of Protease trans-Cleavage and Helicase Activities in Pestivirus NS3.Journal of Virology (IF 6.549) Pub Date : 2017-08-25 ,DOI:10.1128/jvi.01094-17Fengwei Zheng,Guoliang Lu,Ling Li,Peng Gong,Zishu Pan
The nonstructural protein NS3 from the Flaviviridae family is a multifunctional protein that contains an N-terminal protease and a C-terminal helicase, playing essential roles in viral polyprotein processing and genome replication. Here we report a full-length crystal structure of the classical swine fever virus (CSFV) NS3 in complex with its NS4A protease cofactor segment (PCS) at a 2.35-Å resolution
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Synergistic roles of the E2 glycoprotein and 3' untranslated region in the increased genomic stability of chimeric classical swine fever virus with attenuated phenotypes.Archives of Virology (IF 2.685) Pub Date : 2017-06-03 ,DOI:10.1007/s00705-017-3427-9Rui Wu,Ling Li,Lei Lei,Cheng Zhao,Xiaofang Shen,Haizhong Zhao,Zishu PanThe E2 glycoprotein and 3' untranslated region (UTR) of classical swine fever virus (CSFV) are virulence determinants. To investigate the synergistic roles of E2 and 3'UTR for pathogenicity and genomic stability, a series of chimeric CSFVs were constructed by replacing the E2 gene and/or 3'UTR of virulent CSFV strain Shimen with the corresponding sequence of the lapinized 'Chinese' strain (C-strain) ...
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Vesicular stomatitis virus-based vaccines expressing EV71 virus-like particles elicit strong immune responses and protect newborn mice from lethal challenges.Vaccine (IF 4.169) Pub Date : 2016-07-05 ,DOI:10.1016/j.vaccine.2016.06.058Qin Yan,Linjuan Wu,Longyun Chen,Yali Qin,Zishu Pan,Mingzhou ChenEnterovirus 71 (EV71) belonging to the Picornaviridae family is considered the most frequently detected causative agent in hand-foot-and-mouth disease (HFMD) and is a serious threat to public health in the Asia-Pacific region. There are currently no approved vaccines or effective drugs for EV71. In this study, using recombinant vesicular stomatitis virus (rVSV) expressing viral VP1 protein (mVP1) of ...
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Chimeric virus-like particles containing a conserved region of the G protein in combination with a single peptide of the M2 protein confer protection against respiratory syncytial virus infection.Antiviral Research (IF 10.103) Pub Date : 2016-05-08 ,DOI:10.1016/j.antiviral.2016.05.001Lei Qiao,Yuan Zhang,Feng Chai,Yiluo Tan,Chunling Huo,Zishu PanTo investigate the feasibility and efficacy of a virus-like particle (VLP) vaccine composed of the conserved antigenic epitopes of respiratory syncytial virus (RSV), the chimeric RSV VLPs HBcΔ-tG and HBcΔ-tG/M282-90 were generated based on the truncated hepatitis B virus core protein (HBcΔ). HBcΔ-tG consisted of HBcΔ, the conserved region (aa 144-204) of the RSV G protein. HBcΔ-tG was combined with ...
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Development of a novel single-step reverse genetics system for the generation of classical swine fever virus.Archives of Virology (IF 2.685) Pub Date : 2016-04-14 ,DOI:10.1007/s00705-016-2851-6Ling Li,Huining Pang,Rui Wu,Yanwen Zhang,Yiluo Tan,Zishu PanWe describe an alternative reverse genetics system for generating classical swine fever virus (CSFV) based on swine RNA polymerase I promoter (pSPI)-mediated vRNA transcription. The recombinant plasmid pSPTI/SM harboring a full-length CSFV Shimen strain cDNA, flanked by a swine RNA polymerase I (pol I) promoter sequence at the 5' end and a murine pol I terminator sequence at the 3' end, was constructed ...
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Molecular basis for the thermostability of Newcastle disease virus.Scientific Reports (IF 4.996) Pub Date : 2016 ,DOI:10.1038/srep22492Guoyuan Wen,Xiao Hu,Kang Zhao,Hongling Wang,Zhenyu Zhang,Tengfei Zhang,Jinlong Yang,Qingping Luo,Rongrong Zhang,Zishu Pan,Huabin Shao,Qingzhong Yu
Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain and
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Molecular basis for the thermostability of Newcastle disease virus.Scientific Reports (IF 4.996) Pub Date : 2016-Mar-0 ,DOI:10.1038/srep22492Guoyuan Wen,Xiao Hu,Kang Zhao,Hongling Wang,Zhenyu Zhang,Tengfei Zhang,Jinlong Yang,Qingping Luo,Rongrong Zhang,Zishu Pan,Huabin Shao,Qingzhong Yu
Thermostable Newcastle disease virus (NDV) vaccines have been used widely to protect village chickens against Newcastle disease, due to their decreased dependence on cold chain for transport and storage. However, the genetic basis underlying the NDV thermostability is poorly understood. In this study, we generated chimeric viruses by exchanging viral genes between the thermostable TS09-C strain and
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Japanese encephalitis virus induces apoptosis by the IRE1/JNK pathway of ER stress response in BHK-21 cells.Archives of Virology (IF 2.685) Pub Date : 2015-12-15 ,DOI:10.1007/s00705-015-2715-5Mingjie Huang,Ahui Xu,Xiaoyu Wu,Yanni Zhang,Yunli Guo,Fenglin Guo,Zishu Pan,Lingbao KongThe role of endoplasmic reticulum (ER) stress in Japanese encephalitis is largely unknown. In this study, we found that Japanese encephalitis virus (JEV) strain SA14-14-2 regulates the expression of glucose-regulated protein 78 (GRP78), transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP), and splicing of X-box-binding protein 1 (XBP1) mRNA in BHK-21 cells. SA14-14-2-induced cytopathic ...
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Baculovirus vectors expressing F proteins in combination with virus-induced signaling adaptor (VISA) molecules confer protection against respiratory syncytial virus infection.Vaccine (IF 4.169) Pub Date : 2015-12-09 ,DOI:10.1016/j.vaccine.2015.11.027Yuan Zhang,Lei Qiao,Xiao Hu,Kang Zhao,Yanwen Zhang,Feng Chai,Zishu PanBaculovirus has been exploited for use as a novel vaccine vector. To investigate the feasibility and efficacy of recombinant baculoviruses (rBVs) expressing respiratory syncytial virus (RSV) fusion (F) proteins, four constructs (Bac-tF/64, Bac-CF, Bac-CF/tF64 and Bac-CF/tF64-VISA) were generated. Bac-tF64 displays the F ectodomain (tF) on the envelope of rBVs, whereas Bac-CF expresses full-length F ...
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Respiratory Syncytial Virus Nonstructural Proteins Upregulate SOCS1 and SOCS3 in the Different Manner from Endogenous IFN Signaling.Journal of Immunology Research (IF 4.493) Pub Date : 2015-11-12 ,DOI:10.1155/2015/738547Junwen Zheng,Pu Yang,Yan Tang,Zishu Pan,Dongchi Zhao
Respiratory syncytial virus (RSV) infection upregulates genes of the suppressor of cytokine signaling (SOCS) family, which utilize a feedback loop to inhibit type I interferon dependent antiviral signaling pathway. Here, we reconstituted RSV nonstructural (NS) protein expression plasmids (pNS1, pNS2, and pNS1/2) and tested whether NS1 or NS2 would trigger SOCS1 and SOCS3 protein expression. These NS
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