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A Review: Molecular Chaperone-mediated Folding, Unfolding and Disaggregation of Expressed Recombinant Proteins Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-02-25 Komal Fatima, Fatima Naqvi, Hooria Younas
The advancements in biotechnology over time have led to an increase in the demand of pure, soluble and functionally active proteins. Recombinant protein production has thus been employed to obtain high expression of purified proteins in bulk. E. coli is considered as the most desirable host for recombinant protein production due to its inexpensive and fast cultivation, simple nutritional requirements
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Laminin-411 and -511 Modulate the Proliferation, Adhesion, and Morphology of Gastric Cancer Cells Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-02-25 Masaya Iwamuro, Hidenori Shiraha, Atsushi Oyama, Daisuke Uchida, Shigeru Horiguchi, Hiroyuki Okada
Laminins (Ln), a type of extracellular matrix glycoprotein, are key regulators of cellular behavior. Recent work revealed that in various tumor cell lines, laminin isoforms influence specific responses, such as cell anchorage, survival, proliferation, migration, organization, and specialization. The contribution of laminin isoforms to the function of gastric cancer cells, however, remain unclear. Here
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Chelerythrine Ameliorates Pulmonary Fibrosis via Activating the Nrf2/ARE Signaling Pathway Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-02-12 Ling Peng, Li Wen, Qingfeng Shi, Feng Gao, Bin Huang, Changming Wang
Chelerythrine (CHE) is a natural benzophenanthridine alkaloid, which has shown its anti-fibrosis activity in kidney and liver, while the impact of CHE in pulmonary fibrosis is still unclear. This study is developed to explore the impact and mechanism of CHE in pulmonary fibrosis. Pulmonary fibrosis mouse models were established through intratracheal injection of bleomycin (BLM), after which the mice
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Functional Regulatory Mechanisms Underlying Bone Marrow Mesenchymal Stem Cell Senescence During Cell Passages Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-02-09 T. Iwata, N. Mizuno, S. Ishida, M. Kajiya, T. Nagahara, E. Kaneda-Ikeda, M. Yoshioka, S. Munenaga, K. Ouhara, T. Fujita, H. Kawaguchi, H. Kurihara
Mesenchymal stem cell (MSC) transplantation is an effective periodontal regenerative therapy. MSCs are multipotent, have self-renewal ability, and can differentiate into periodontal cells. However, senescence is inevitable for MSCs. In vitro, cell senescence can be induced by long-term culture with/without cell passage. However, the regulatory mechanism of MSC senescence remains unclear. Undifferentiated
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Molecular dynamics, residue network analysis, and cross-correlation matrix to characterize the deleterious missense mutations in GALE causing galactosemia III Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-02-08 S. Udhaya Kumar, Srivarshini Sankar, D. Thirumal Kumar, Salma Younes, Nadin Younes, R. Siva, C. George Priya Doss, Hatem Zayed
Epimerase-deficiency galactosemia (EDG) is caused by mutations in the UDP-galactose 4’-epimerase enzyme, encoded by gene GALE. Catalyzing the last reaction in the Leloir pathway, UDP-galactose-4-epimerase catalyzes the interconversion of UDP-galactose and UDP-glucose. This study aimed to use in-depth computational strategies to prioritize the pathogenic missense mutations in GALE protein and investigate
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Nitrosonium Cation as a Cytotoxic Component of Dinitrosyl Iron Complexes with Thiol-containing Ligands (based on the Experimental Work on MCF7 Human Breast Cancer Cell Culture) Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-25 Anatoly F. Vanin, Viktor A. Tronov, Rostislav R. Borodulin
Here we demonstrate that binuclear dinitrosyl iron complexes with thiol-containing ligands (glutathione and mercaptosuccinate, B-DNIC-GSH and B-DNIC-MS, respectively) exert cytotoxic effects on MCF7 human breast cancer cells. We showed that they are mediated by nitrosonium cations released from these complexes (NO+). This finding is supported by the cytotoxic effect of both B-DNICs on MCF7 cells evidenced
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UV Radiation-induced Impairment of Cellular Morphology and Motility is Enhanced by DUSP3/VHR Loss and FAK Activation Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-21 Nadine Ranieri Pereira, Lilian Cristina Russo, Fabio Luis Forti
DUSP3 is a phosphatase expressed and active in several tissues that dephosphorylates tyrosine residues in many regulatory proteins of cellular activities such as proliferation, survival, and cell death. Recently, two new independent functions were assigned to this enzyme: dephosphorylation of focal adhesion kinase (FAK) and regulation of nucleotide-excision repair (NER) pathway. Genotoxic stress by
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Morphometry and Modeling of Label-Free Human Melanocytes and Melanoma Cells Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-14 Sharareh Tavaddod, Behnaz Shojaedin-Givi, Mahnaz Mahmoudi-Rad, Hossein Naderi-Manesh
A combination of light microscopy and image processing was applied to investigate morphology of label-free primary-melanocytes and melanoma cells. A novel methodological approach based on morphology of nuclear body was used to find those single cells, which were at the same phase of cell cycle. The area and perimeter of melanocytes and melanoma cells were quantified. We found that there was a significant
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Vitamin E Derivative with Modified Side Chain Induced Apoptosis by Modulating the Cellular Lipids and Membrane Dynamics in MCF7 Cells Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-13 Seher Gok, Oleksandr Kuzmenko, Andrii Babinskyi, Feride Severcan
The vitamin E derivative with side chain modification (TC6OAc) has been shown to possess anticancer activity in our earlier in vivo studies. It was hypothesized that, as Vitamin E (VE) and VE derivative are fat soluble lipophilic molecules, they exert their function by modulating the lipid metabolism and related pathways. This study aimed to evaluate the cellular impact of this VE derivative (2,5,7
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Acteoside Presents Protective Effects on Cerebral Ischemia/reperfusion Injury Through Targeting CCL2, CXCL10, and ICAM1 Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-13 Weijiang Wu, Gang Wu, Deyan Cao
The objective of this study is to investigate the roles of acteoside (ACT) in cells with oxygen–glucose deprivation and reoxygenation (OGD/R)-induced injury and the underlying mechanisms. The differentially expressed genes (DEGs) in rats with middle cerebral artery occlusion were identified using GSE61616 data set. Kyoto Encyclopedia of Genes and Genomes pathway enrichment with the DEGs and the prediction
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Deciphering Biophysical Modulation in Ovarian Cancer Cells Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-12 Makhdoom Sarwar, Peter H. Sykes, Kenny Chitcholtan, John J. Evans
It has been long known that the oncogenic extracellular environment plays an indispensable role in developing and nurturing cancer cell progression and in resistance to standard treatments. However, by how much the biophysical components of tumour extracellular environment contribute to these processes is uncertain. In particular, the topographic environment is scarcely explored. The biophysical modulation
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Obestatin and Rosiglitazone Differentially Modulate Lipid Metabolism Through Peroxisome Proliferator-activated Receptor-γ (PPARγ) in Pre-adipose and Mature 3T3-L1 Cells Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-11 Mallikarjuna B. G., Uma V. Manjappara
Obestatin is a 23-residue peptide, obtained after posttranslational modification of preproghrelin. It has been shown, in Swiss albino mice, to upregulate glycerolipid metabolism and PPARγ signaling. It was opined that the by-products of increased glycerolipid metabolism triggered PPARγ signaling. It was hypothesized that obestatin upon co-administration with a full agonist of PPARγ should reveal the
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CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway Cell Biochem. Biophys. (IF 2.073) Pub Date : 2021-01-11 Dezhao Lu, Yifei Le, Jiali Ding, Xiaobing Dou, Wei Mao, Ji Zhu
Chloride intracellular channel 1 (CLIC1) is a sensor of oxidative stress in endothelial cells (EC). However, the mechanism by which CLIC1 mediate the regulation of endothelial dysfunction has not been established. In this study, overexpressed CLIC1 impaired the ability of the vascular cells to resist oxidative damage and promoted cellular senescence. Besides, suppressed CLIC1 protected against cellular
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Therapeutic Path to Double Knockout: Investigating the Selective Dual-Inhibitory Mechanisms of Adenosine Receptors A1 and A2 by a Novel Methoxy-Substituted Benzofuran Derivative in the Treatment of Parkinson’s Disease Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-11-22 Temitayo I. Subair, Opeyemi S. Soremekun, Fisayo A. Olotu, Mahmoud E. S. Soliman
The dual inhibition of adenosine receptors A1 (A1 AR) and A2 (A2A AR) has been considered as an efficient strategy in the treatment of Parkinson’s disease (PD). This led to the recent development of a series of methoxy-substituted benzofuran derivatives among which compound 3j exhibited dual-inhibitory potencies in the micromolar range. Therefore, in this study, we seek to resolve the mechanisms by
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Simultaneous Determination of Human Erythrocyte Deformability and Adhesion Energy: A Novel Approach Using a Microfluidic Chamber and the “Glass Effect” Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-11-07 Carolina M. Londero, Bibiana D. Riquelme
The simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was proposed. Measurements were carried out with red blood cells suspended in saline solution
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The Novel Target of Colorectal Carcinoma: TRIM44 Regulates Cell Migration and Invasion via Activation of CXCR4/NF-κB Signaling Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-11-05 Tianlei Kou, Deqian Sha, Fei Wang, Tao He, Xuan He
Tripartite motif containing 44 (TRIM44) has been reported to regulate various biological effects in malignant cancers and matrix Metalloproteinases has been demonstrated to be associated with cancer cell migration and invasion. Nonetheless, the expression and molecular mechanism of TRIM44 in colorectal cancer (CRC) remain rarely known. TRIM44 was overexpressed or knocked down in CRC cells. Subsequently
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Icaritin Inhibits Skin Fibrosis through Regulating AMPK and Wnt/β-catenin Signaling Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-30 Ming Li, Qingmei Liu, Shan He, Xiangzhen Kong, Jinpei Lin, Yan Huang, Wenyu Wu, Jinfeng Wu
Skin fibrosis is one of the major features of scleroderma. WNT/β-catenin signaling is associated with the progression of skin fibrosis. In this study, we aimed to determine the effect of icaritin (IT), a natural compound, on scleroderma-related skin fibrosis and its mechanisms. We found that IT could reduce the expression of COL1A1, COL1A2, COL3A1, CTGF, and α-SMA in human foreskin fibroblasts (HFF-1
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Downregulation of N-myc Interactor Promotes Cervical Cancer Cells Growth by Activating Stat3 Signaling Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-27 Songbin Wu, Xiaotian Li, Huizi Chai, Linyuan Feng, Wenjing Li, Hongjian Li
N-myc interactor (NMI), a member of the oncogene Myc family, has been reported to be closely related to the development of cancer. However, the character of NMI in cervical carcinoma has not been reported. Herein, we found that downregulation of NMI protein not only promoted the proliferation, migration, and invasion of HeLa cells, but also decreased their expression of Caspase-3 and Caspase-9. Silencing
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PKCζ–NADPH Oxidase–PKCα Dependent Kv1.5 Phosphorylation by Endothelin-1 Modulates Nav1.5–NCX1–Cav1.2 Axis in Stimulating Ca 2+ Level in Caveolae of Pulmonary Artery Smooth Muscle Cells Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-23 Jaganmay Sarkar, Tapati Chakraborti, Pijush Kanti Pramanik, Priyanka Ghosh, Amritlal Mandal, Sajal Chakraborti
Endothelin-1 (ET-1) is a potent endogenously derived vasoconstrictor, which increases pulmonary hypertension via stimulation of [Ca2+]i level in pulmonary artery smooth muscle cells (PASMCs). In this communication, we sought to investigate the mechanism by which ET-1 causes stimulation of Ca2+ concentration in caveolae vesicles of bovine PASMCs (BPASMCs). ET-1 activates PKC-α in the caveolae vesicles
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Evaluation of Toxic Effects of Novel Platinum (IV) Complexes in Female Rat Liver: Potential Protective Role of Resveratrol Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-22 Milica G. Paunović, Miloš M. Matić, Vesna D. Stanković, Marija D. Milošević, Verica V. Jevtić, Srećko R. Trifunović, Branka I. Ognjanović
The use of cisplatin in chemotherapy may provoke a deteriorating impact in many vital organs, suggesting the need for more selective derivatives and effective protective cotreatments. This study assesses the effects of three novel Pt(IV) complexes containing ethyl-, propyl- and butyl-esters of the ethylenediamine-N, N’-di-S, S- (2,2’-dibenzyl) acetic acid on liver injury markers, redox parameters,
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Liver Cancer Cells Uptake Labile Iron via L-type Calcium Channel to Facilitate the Cancer Cell Proliferation Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-16 Isara Phiwchai, Titipun Thongtem, Somchai Thongtem, Chalermchai Pilapong
Herein, we studied the effect of labile iron (ferric chloride) on the progression of liver cancer cells (HepG2.2.15). The iron was found to induce cell proliferation, growth, and migration in both traditional two-dimensional (2D) and three-dimensional cell (3D) culture models. Biophysical and cell cycle determinations also showed the change in functional cellular biophysical features (cell morphology)
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Molecular Basis of P131 Cryptosporidial-IMPDH Selectivity—A Structural, Dynamical and Mechanistic Stance Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-15 Kehinde F. Omolabi, Clement Agoni, Fisayo A. Olotu, Mahmoud E. S. Soliman
Cryptosporidiosis accounts for a surge in infant (<5 years) mortality and morbidity. To date, several drug discovery efforts have been put in place to develop effective therapeutic options against the causative parasite. Based on a recent report, P131 spares inosine monophosphate dehydrogenase (IMPDH) in a eukaryotic model (mouse IMPDH (mIMPDH)) while binding selectively to the NAD+ site in Cryptosporidium
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In Silico Prediction of Metabolic Fluxes in Cancer Cells with Altered S-adenosylmethionine Decarboxylase Activity Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-11 Olga Dotsenko, Dmytro Shtofel
This paper investigates the redistribution of metabolic fluxes in the cell with altered activity of S-adenosylmethionine decarboxylase (SAMdc, EC: 4.1.1.50), the key enzyme of the polyamine cycle and the common target for antitumor therapy. To address these goals, a stoichiometric metabolic model was developed that includes five metabolic pathways: polyamine, methionine, methionine salvage cycles,
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Lipids Composition in Plant Membranes Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-09 Emilia Reszczyńska, Agnieszka Hanaka
The paper focuses on the selected plant lipid issues. Classification, nomenclature, and abundance of fatty acids was discussed. Then, classification, composition, role, and organization of lipids were displayed. The involvement of lipids in xantophyll cycle and glycerolipids synthesis (as the most abundant of all lipid classes) were also discussed. Moreover, in order to better understand the biomembranes
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NK6 Homeobox 2 Regulated Gastrokin-2 Suppresses Gastric Cancer Cell Proliferation and Invasion via Akt Signaling Pathway Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-10-03 Jin Dai, Tieli Peng, Xiangdi Yu
This study aims to explore the role of Gastrokin-2 (GKN2) in gastric cancer and its function in the progression and metastasis of gastric cancer. The expression of GKN2 in the patient samples was examined by qRT-PCR and western blot. The transcription factor NK6 Homeobox 2 (NKX6-2), which binds to the GKN2 promoter, was predicted by cBioportal and JSPAR. Binding between NKX6-2 and the GKN2 promoter
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Effect of Tween Type Non-Ionic Detergent on the Activity of Lipase of Pseudomonas aeruginosa Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-30 Maryam Shoja, Dariush Minai-Tehrani
Pseudomonas aeruginosa is a Gram-negative and rod-shaped bacterium. It can use a variety of carbon sources and grow in different culture media. Its versatile extracellular enzymes give it the ability to grow on complex carbon sources. One of the most important enzymes of this bacterium is lipase, which is an extracellular enzyme. Lipases are one of the most useful enzymes in medicine and industry,
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What is the Role of Lipid Membrane-embedded Quinones in Mitochondria and Chloroplasts? Chemiosmotic Q-cycle versus Murburn Reaction Perspective Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-29 Kelath Murali Manoj, Daniel Andrew Gideon, Abhinav Parashar
Quinones are found in the lipid membranes of prokaryotes like E. coli and cyanobacteria, and are also abundant in eukaryotic mitochondria and chloroplasts. They are intricately involved in the reaction mechanism of redox phosphorylations. In the Mitchellian chemiosmotic school of thought, membrane-lodged quinones are perceived as highly mobile conveyors of two-electron equivalents from the first leg
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Monosodium Glutamate Induces Cytotoxicity in Rat Liver via Mitochondrial Permeability Transition Pore Opening. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-23 Adeola Oluwakemi Olowofolahan,Oluwatobi Andrew Adeosun,Olufunso Olabode Olorunsogo
Monosodium glutamate (MSG) is a major food additive used as a flavor enhancer. A lot of controversies have been generated over the use of MSG. The present study therefore investigated whether MSG would induce cytotoxicity via the induction of mitochondrial permeability transition (mPT) pore opening. 36 male albino rats were used for this study. The rats were equally divided into six groups: group I
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The Relevance of SNPs at 3'UTR Region of CASP7 and miR-371b-5p Associated Diseases: A Computational Analysis. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-19 Muhammad Safdar,Sana Zaheer,Rozhgar A Khailany,Sadaf Parvez,Muhammad Naveed,Piplu Bhuiyan,Mehmet Ozaslan,Rebaz Moatasam,Mustafa S Al-Attar,Musarrat Abbas Khan,Yasmeen Junejo
The process of genetically programmed cell death, or apoptosis, plays a crucialrolein cellular homeostasis and gene expression. Disruption of apoptosis may lead to aberrant immune responses, cancer, and neurodegenerative diseases. Single nucleotide polymorphisms (SNPs) present in various microRNA (miRNA) genes and targets being an alteration of miRNA activity resulting in human diseases. Evidence reported
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Photoreactivity of Bis-retinoid A2E Complexed with a Model Protein in Selected Model Systems. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-12 Justyna Furso,Andrzej Zadlo,Grzegorz Szewczyk,Tadeusz J Sarna
The bis-retinoid N-retinyl-N-retinylidene ethanolamine (A2E) is formed as a byproduct of visual cycle in retinal pigment epithelium (RPE). It contributes to golden-yellow fluorescence of the age pigment lipofuscin, which accumulates in RPE. Lipofuscin can generate a variety of reactive oxygen species (ROS) upon blue-light excitation. Although in model systems photoreactivity of A2E has been determined
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Cathepsin V Mediates the Tazarotene-induced Gene 1-induced Reduction in Invasion in Colorectal Cancer Cells. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-12 Chun-Hua Wang,Lu-Kai Wang,Chang-Chieh Wu,Mao-Liang Chen,Chan-Yen Kuo,Rong-Yaun Shyu,Fu-Ming Tsai
Tazarotene-induced gene 1 (TIG1) is a retinoid acid receptor-responsive gene involved in cell differentiation and tumorigenesis. Aberrant methylation of CpG islands in the TIG1 promoter is found in multiple cancers. Currently, the exact mechanism underlying the anticancer effect of TIG1 is unknown. Here, we show that TIG1 interacts with cathepsin V (CTSV), which reduces CTSV stability and subsequently
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HuR Promotes Ovarian Cancer Cell Proliferation by Regulating TIMM44 mRNA Stability. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-08 Xiaohui Yu,Yujiao Li,Yumei Ding,Hong Zhang,Ning Ding,Ming Lu
The human antigen R (HuR) could play an essential role in stabilizing the mRNAs of many tumor-associated genes. Little research is performed to investigate the relevant mechanism mediated by HuR to promote the progress of ovarian cancer. The Cancer Genome Atlas (TCGA) dataset was retrieved to calculate the correlation between HuR and translocase of inner mitochondrial membrane 44 (TIMM44) expression
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Tocopherol Moderately Induces the Expressions of Some Human Sulfotransferases, which are Activated by Oxidative Stress. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-09-08 Sangita MaitiDutta,Guangping Chen,Smarajit Maiti
Oxidative stress is generated in biological system by several endogenous/exogenous factors like environmental-pollution/toxicity/diseases and by daily-life-stress. We previously showed that oxidative-stress impaired the activities/expressions of phase-II drug-metabolizing enzyme, sulfotransferases (SULTs). The SULT catalyzes sulfation of endogenous/exogenous compounds. Vitamin E is globally consumed
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Pharmacological Properties of Pt(II) and Pt(IV) Complexes with 2,2'-Dipyridylamine; the Comparative In Vitro Thereof. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-08-27 Simin Aghmasheh,S Nasser Ostad,Anita Abedi
Pt-based drugs such as cisplatin are frontline drugs used for the treatment of different solid malignancies. However, they represent major problems, such as severe side effects and drug resistance. To find out the structure–activity relationship; in this study, Pt(II) and Pt(IV) complexes with similar ligands, namely tetrachloro(2,2′-dipyridylamine)platinum(IV) (1) and dichloro(2,2′-dipyridylamine)platinum(II)
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Expression Analysis of GDNF/RET Signaling Pathway in Human AD-MSCs Grown in HEK 293 Conditioned Medium (HEK293-CM). Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-08-14 Zahra Esmaeilizadeh,Bahar Mohammadi,Masoumeh Rajabibazl,Sayyed Mohammad Hossein Ghaderian,Mir Davood Omrani,Zahra Fazeli
Mesenchymal stem cells have been considered as the suitable source for the repair of kidney lesions. The study and identification of novel approaches could improve the efficiency of these cells in the recovery of kidney. In the present study, the effect of HEK 293 conditioned medium (HEK293-CM) was evaluated on the expression of GDNF/RET signaling pathway and their downstream genes in the human adipose-derived
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Contrary Roles of Wnt/β-Catenin Signaling in BMP9-Induced Osteogenic and Adipogenic Differentiation of 3T3-L1 Preadipocytes. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-27 Kailu Liang,Yu Du,Liang Chen,Liyuan Wang,Ruidong Li,Zhengjian Yan,Yang Liu
Our previous study revealed that 3T3-L1 preadipocytes can differentiate to either osteoblasts or adipocytes in response to bone morphogenic protein 9 (BMP9). In the present study, we try to further investigate whether the Wnt/β-catenin signaling plays a crucial role in this process. It was found that BMP9 effectively activated the Wnt/β-catenin signaling, and induced the expression levels of certain
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Hydroxysafflor Yellow A of Carthamus Tinctorius L., Represses the Malignant Development of Esophageal Cancer Cells via Regulating NF-κB Signaling Pathway. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-23 Xiumei Chen,Yanfang Wang,Lianjun Zhang,Yujun Gao
Esophageal cancer (EC) is a common digestive tract malignant tumor and the clinical outcome of patients with EC after surgery remains unsatisfactory. Hence, it is necessary to identify some effective drugs or methods to improve the prognosis of patients with EC. In this study, we attempted to analyze the potential role of hydroxysafflor yellow A (HSYA) in EC. Combined with The Cancer Genome Atlas (TCGA)
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Simultaneous Disruption of Both Polyubiquitin Genes Affects Proteasome Function and Decreases Cellular Proliferation. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-23 Chul-Woo Park,Jin-Sil Bae,Kwon-Yul Ryu
The ubiquitin (Ub) proteasome system is important for maintaining protein homeostasis and has various roles in cell signaling, proliferation, and cell cycle regulation. In mammals, Ub is encoded by two monoubiquitin and two polyubiquitin genes. Although reduced levels of Ub due to the disruption of one polyubiquitin gene are known to decrease cell proliferation, the effect of disrupting both polyubiquitin
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Low Shear Stress Upregulates CX3CR1 Expression by Inducing VCAM-1 via the NF-κB Pathway in Vascular Endothelial Cells. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-19 Yiwei Zhao,Peile Ren,Qiufang Li,Shafiu Adam Umar,Tan Yang,Yahui Dong,Fengxu Yu,Yongmei Nie
Atherosclerosis is a significant cause of mortality and morbidity. Studies suggest that the chemokine receptor CX3CR1 plays a critical role in atherogenesis. Shear stress is an important mechanical force that affects blood vessel function. In this study, we investigated the effect of shear stress on CX3CR1 expression in vascular endothelial cells (VECs). First, cells were exposed to different shear
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Lowered Expression of MicroRNAs 221 and 222 Mediate Apoptosis Induced by High Glucose in Human Periodontal Ligament Cells. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-17 Mariana M Monteiro,Cilene R Lima,Cibele C Gomes,Mario C Cruz,Anna C R T Horliana,Marinilce F Santos
Impaired periodontal healing is a common complication of diabetes mellitus (DM), frequently related to hyperglycemia. MicroRNAs 221 and 222 have been studied as biomarkers for inflammatory diseases, including diabetes, but their role in the periodontal ligament (PL) is unknown. The effects of high glucose on human PL cells death were studied, as well as the expression of microRNA-221 and microRNA-222
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Lentivirus-Mediated Overexpression of SIVA-1 Reverses Cisplatin Resistance in Gastric Cancer in vitro. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-09 Xiao-Tong Wang,Lei Li,Fan-Biao Kong,Xiao-Gang Zhong,Wei Mai
SIVA-1 plays a critical role in the induction of apoptosis in a number of different cell lines and participates in the mechanism of cisplatin (DDP)-mediated antitumor effects. However, the involvement of SIVA-1 in cisplatin resistance in gastric carcinoma has not been revealed. To explore the effect of SIVA-1 on DDP resistance, a recombinant pGV358-GFP-SIVA-1 lentiviral vector was constructed and transfected
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MT2A Promotes Oxaliplatin Resistance in Colorectal Cancer Cells. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-07 Zhicheng Zhao,Guojing Zhang,Weidong Li
We aimed to understand the molecular mechanism underlying the incidence of Oxaliplatin resistance in colorectal cancer. The Oxaliplatin-resistant (OR) HT29 colorectal cell line was established by long-term exposure to Oxaliplatin. Cell viability and proliferation were determined by the 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide and direct counting assays, respectively. Transcript
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The Role of Arachidonic Acid Metabolism in Myocardial Ischemia-Reperfusion Injury. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-07-04 Changjiang Zhang,Meiling He,Lihua Ni,Ke He,Ke Su,Yinzhi Deng,Yuanhong Li,Hao Xia
Patients with myocardial ischemic diseases or who are undergoing one of various heart treatments, such as open heart surgery, coronary artery bypass grafting, percutaneous coronary artery intervention or drug thrombolysis, face myocardial ischemia–reperfusion injury (MIRI). However, no effective treatment is currently available for MIRI. To improve the prognosis of people with cardiovascular disease
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Hypothetical Pathway for Formation of Cholesterol Microcrystals Initiating the Atherosclerotic Process. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-30 Witold K Subczynski,Marta Pasenkiewicz-Gierula
Major factors leading to the development of atherosclerosis are a high cholesterol (Chol) level in the blood and oxidative stress. Both promote the formation of Chol microcrystals in blood vessel walls. Deposition of Chol microcrystals in arterial intima causes inflammation, which initiates and accompanies the atherosclerotic process in all its phases. One of the possible sources of Chol in the blood
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'Polymorphism-aided' Selective Targeting and Inhibition of Caspase-6 by a Novel Allosteric Inhibitor Towards Efficient Alzheimer's Disease Treatment. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-26 Ransford Oduro Kumi,Clement Agoni,Abdul Rashid Issahaku,Fisayo A Olotu,Mahmoud E S Soliman
The predominance of Alzheimer’s disease (AD) among the aged remains a global challenge. As such, the search for alternative and effective therapeutic options continuous unabated. Among the therapeutic targets explored over the years toward impeding the progression of AD is caspase-6 (Casp6), although selectively targeting Casp6 remains a challenge due to high homology with other members of the caspase
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Structural and Functional Characterisation of the Domains of Ubiquitin-Activating Enzyme (E1) of Saccharomyces cerevisiae. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-24 Brinda Panchamia,Varsha Raimalani,Vishal Prashar,Mukesh Kumar,C Ratna Prabha
Ubiquitin-activating enzyme (E1) is the first enzyme of the ubiquitination pathway and is required to activate ubiquitin. E1 of Saccharomyces cerevisiae is a large multidomain monomeric protein. There are no studies available on the domains of yeast E1 as independent entities. Four domains of E1 namely, first catalytic cysteine half-domain (FCCH), four-helix bundle (4HB), second catalytic cysteine
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Prediction of Ubiquitin Ligase Nrdp1-Associated Proteins in Glioma Database. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-19 Yong Liu,Mingwei Jin,Yong Gao,Yuan Wang,Shengbai Xue,Lei Wang,Chengmin Xuan
The ubiquitin proteasome pathway is conserved from yeast to mammals and is necessary for the targeted degradation of most short-lived proteins in eukaryotic cells. Its protein substrates include cell cycle regulatory proteins and proteins that are not properly folded in the endoplasmic reticulum. Owing to the ubiquity of its protein substrates, ubiquitination regulates a variety of cellular activities
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An Update on the Pharmacological Usage of Curcumin: Has it Failed in the Drug Discovery Pipeline? Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-05 Fisayo Olotu,Clement Agoni,Opeyemi Soremekun,Mahmoud E S Soliman
The pharmacological propensities of curcumin have been reported in a plethora of pre-clinical and clinical studies. However, innate attributes account for extremely low oral bioavailability which impedes its development as a therapeutic agent. Regardless, these drawbacks have not deterred researchers from optimizing its potentials. This review discussed the pharmacokinetic properties of curcumin relative
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Fumonisin B1 Affects the Biophysical Properties, Migration and Cytoskeletal Structure of Human Umbilical Vein Endothelial Cells. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-05 Xue Zhao,Yun Wang,Jiang-Li Liu,Jian-Hua Zhang,Shi-Chao Zhang,Yan Ouyang,Jiang-Tao Huang,Xiao-Yan Peng,Zhu Zeng,Zu-Quan Hu
Fumonisin B1 (FB1) is an important mycotoxin in nature and is a serious threat to human and animal health, but its specific target and molecular mechanism of the toxicity and potential carcinogenicity remain unclear. In this study, we first detected the effects of FB1 on the cell viability, biophysical properties, migration ability, and reactive oxygen species (ROS) of human umbilical vein endothelial
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An EPR Study Using Cyclic Hydroxylamines To Assess The Level of Mitochondrial ROS in Superinvasive Cancer Cells. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-06-01 Samantha Scheinok,Tania Capeloa,Paolo E Porporato,Pierre Sonveaux,Bernard Gallez
It has been proposed that a mitochondrial switch involving a high mitochondrial superoxide production is associated with cancer metastasis. We here report an EPR analysis of ROS production using cyclic hydroxylamines in superinvasive SiHa-F3 compared with less invasive SiHa wild-type human cervix cancer cells. Using the CMH probe, no significant difference was observed in the overall level of ROS between
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C-terminal Tail of β-Tubulin and its Role in the Alterations of Dynein Binding Mode. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-27 Kali A Heale,Laleh Alisaraie
Dynein is a cytoskeletal molecular motor protein that moves along the microtubule (MT) and transports various cellular cargos during its movement. Using standard Molecular Dynamics (MD) simulation, Principle Component Analysis (PCA), and Normal Mode Analysis (NMA) methods, this investigation studied large-scale movements and local interactions of dynein’s Microtubule Binding Domain (MTBD) when bound
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XIth EPR Workshop on Applications of EPR in Biology and Medicine. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-25 Lawrence Berliner,Tadeusz Sarna,Balaraman Kalyanaraman
We are pleased and honored to present this special issue for CBBI on the broad topic of biomedical EPR. The papers herein resulted from the most recent October 2019 EPR Workshop in Kraków that encompasses work from outstanding researchers in the field. Before describing the range of articles, we have briefly summarized the history of these workshops and the publications that resulted.
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The influence of iron on selected properties of synthetic pheomelanin. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-24 Andrzej Zadlo,Krystian Mokrzyński,Shosuke Ito,Kazumasa Wakamatsu,Tadeusz Sarna
It is believed that while eumelanin plays photoprotective and antioxidant role in pigmented tissues, pheomelanin being more photoreactive could behave as a phototoxic agent. Although the metal ion-sequestering ability of melanin might be protective, transition metal ions present in natural melanins could affect their physicochemical properties. The aim of this research was to study iron binding by
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Selective Induction of Cellular Toxicity and Anti-tumor Efficacy by N-Methylpiperazinyl Diarylidenylpiperidone and its Pro-nitroxide Conjugate through ROS-mediated Mitochondrial Dysfunction and G2/M Cell-cycle Arrest in Human Pancreatic Cancer. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-24 Jesse M Mast,John W Hinds,Dan Tse,Katherine Axelrod,M Lakshmi Kuppusamy,Maciej M Kmiec,Balázs Bognár,Tamás Kálai,Periannan Kuppusamy
Pancreatic adenocarcinoma is an aggressive cancer with poor clinical prognosis and limited therapeutic options. There is a significant lack of effective, safe, and targeted therapies for successful treatment of pancreatic cancer. In this report, we describe the anticancer efficacy of two novel compounds, N-methylpiperazinyl diarylidenylpiperidone (L-2663) and its pro-nitroxide conjugate (HO-4589) evaluated
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Photoprotective Properties of Vitamin D and Lumisterol Hydroxyderivatives. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-22 Andrzej T Slominski,Anyamanee Chaiprasongsuk,Zorica Janjetovic,Tae-Kang Kim,Joanna Stefan,Radomir M Slominski,Vidya Sagar Hanumanthu,Chander Raman,Shariq Qayyum,Yuwei Song,Yuhua Song,Uraiwan Panich,David K Crossman,Mohammad Athar,Michael F Holick,Anton M Jetten,Michal A Zmijewski,Jaroslaw Zmijewski,Robert C Tuckey
We have previously described new pathways of vitamin D3 activation by CYP11A1 to produce a variety of metabolites including 20(OH)D3 and 20,23(OH)2D3. These can be further hydroxylated by CYP27B1 to produce their C1α-hydroxyderivatives. CYP11A1 similarly initiates the metabolism of lumisterol (L3) through sequential hydroxylation of the side chain to produce 20(OH)L3, 22(OH)L3, 20,22(OH)2L3 and 24(OH)L3
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Effects and Mechanisms of Autophagy Induced by Solubilized-Cholesterol in Hepatocytes: A Comparative Study Among Solvents. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-21 Dong Liang,Ezra K Osoro,Shuai Tan,Xi Lan,Wenhua Zhu,Litao Wu,Xiaojuan Du,Dongmin Li,Shemin Lu
Cholesterol, the principal sterol in mammalian cells, has been reported to play a role in the pathogenesis of several diseases through autophagy. Due to its insoluble characteristic, all in vitro cholesterol experiments are performed using dimethyl sulphoxide, methyl-β-cyclodextrin, and ethanol co-solvents. To investigate whether the types of solvents have different effects on cholesterol-induced cell
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Transcriptomic profiling of long non-coding RNAs in non-virus associated hepatocellular carcinoma. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-14 Lu Liu,Chen He,Haosheng Liu,Ganlu Wang,Zhiwu Lv,Yong Ni,Lisha Mou,Yongqiang Zhan,Jintao Liu
Due to falling prevalence of viral hepatitis (VH), obesity, alcoholism and related liver diseases have become increasingly frequent and important as causes of hepatocellular carcinoma (HCC). However, mechanisms underlying hepatocarcinogenesis and tumor progression in VH-negative HCC remain poorly understood. Long non-coding RNAs (lncRNAs) have been implicated in pathogenesis of human diseases, including
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A Comparative Study of the Action of Protonophore Uncouplers and Decoupling Agents as Inducers of Free Respiration in Mitochondria in States 3 and 4: Theoretical and Experimental Approaches. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-05 Victor N Samartsev,Alena A Semenova,Mikhail V Dubinin
Theoretical and experimental studies have revealed that that in the liver mitochondria an increase in the rate of free respiration in state 3 induced by protonophore uncouplers 2,4-dinitrophenol and сarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone is equal to or slightly greater than the increase in respiration rate in state 4 induced by these uncouplers. In contrast to these protonophore uncouplers
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THH Relieves CIA Inflammation by Reducing Inflammatory-related Cytokines. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-05-03 Xinyi Zhou,Qianqian Liu,Xueqing Zhou,Jie Zhang,Wenhui Liu,Xiangfeng Zhao,Naixiang Luo
Tripterygium hypoglaucum hutch (THH) is a plant of the genus tripterygium, which is also known as colquhounia, Gelsemiun elegan, and so on. It is mainly distributed in Yunnan, Guizhou, and Sichuan regions and other places in China. To study the immune mechanism of THH on related inflammatory cytokines in collagen II-induced arthritis (CIA) mice, healthy male C57BL/6 mice were used to model CIA mice
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Identification and Design of a Next-Generation Multi Epitopes Bases Peptide Vaccine Candidate Against Prostate Cancer: An In Silico Approach. Cell Biochem. Biophys. (IF 2.073) Pub Date : 2020-04-28 Prasanta Patra,Manojit Bhattacharya,Ashish Ranjan Sharma,Pratik Ghosh,Garima Sharma,Bidhan Chandra Patra,Bidyut Mallick,Sang-Soo Lee,Chiranjib Chakraborty
Prostate cancer (PCa) is the second most diagnosed cancer in men and ranked fifth in overall cancer diagnosis. During the past decades, it has arisen as a significant life-threatening disease in men at an older age. At the early onset of illness when it is in localized form, radiation and surgical treatments are applied against this disease. In case of adverse situations androgen deprivation therapy
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