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Generating bright-field images of stained tissue slices from Mueller matrix polarimetric images with CycleGAN using unpaired dataset J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-03-09 Jiahao Fan, Nan Zeng, Honghui He, Chao He, Shaoxiong Liu, Hui Ma
Recently, Mueller matrix (MM) polarimetric imaging-assisted pathology detection methods are showing great potential in clinical diagnosis. However, since our human eyes cannot observe polarized light directly, it raises a notable challenge for interpreting the measurement results by pathologists who have limited familiarity with polarization images. One feasible approach is to combine MM polarimetric
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Reduction of photodynamic damage of blood vessels in the protected region by (–)-epigallocatechin gallate J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-03-09 Tianlong Chen, Yi Shen, Li Lin, Huiyun Lin, Xuejiao Song, Defu Chen, Buhong Li
Photodynamic therapy (PDT) has been increasingly used in the clinical treatment of neoplastic, inflammatory and infectious skin diseases. However, the generation of reactive oxygen species (ROS) may induce undesired side effects in normal tissue surrounding the treatment lesion, which is a big challenge for the clinical application of PDT. To date, (–)-Epigallocatechin gallate (EGCG) has been widely
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Geometric regulation of collective cell tangential ordering migration J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-03-05 Hao Dong, Yuming Zhou, Xuehe Ma, Junfang Liu, Fulin Xing, Jianyu Yang, Qiushuo Sun, Qingsong Hu, Fen Hu, Leiting Pan, Jingjun Xu
Collective cell migration is a coordinated movement of multi-cell systems essential for various processes throughout life. The collective motions often occur under spatial restrictions, hallmarked by the collective rotation of epithelial cells confined in circular substrates. Here, we aim to explore how geometric shapes of confinement regulate this collective cell movement. We develop quantitative
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Spatial sensitivity to absorption changes for various near-infrared spectroscopy methods: A compendium review J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-02-24 Giles Blaney, Angelo Sassaroli, Sergio Fantini
This compendium review focuses on the spatial distribution of sensitivity to localized absorption changes in optically diffuse media, particularly for measurements relevant to near-infrared spectroscopy. The three temporal domains, continuous wave, frequency domain, and time domain, each obtain different optical data types whose changes may be related to effective homogeneous changes in the absorption
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Diagnosis of osteosarcoma based on multimodal microscopic imaging and deep learning J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-02-17 Zihan Wang, Jinjin Wu, Chenbei Li, Bing Wang, Qingxia Wu, Lan Li, Huijie Wang, Chao Tu, Jianhua Yin
Osteosarcoma is the most common primary bone tumor with high malignancy. It is particularly necessary to achieve rapid and accurate diagnosis in its intraoperative examination and early diagnosis. Accordingly, the multimodal microscopic imaging diagnosis system constructed by bright field, spontaneous fluorescence and polarized light microscopic imaging was used to study the pathological mechanism
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Application of cross-polarization imaging in distinguishing between squamous and columnar epithelium of the cervix J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-02-17 Cat Phan Ngoc Khuong, Hieu Nguyen Trung, Duc Le Huynh, Quynh Nguyen Ngoc, Hai Pham Thanh, Long Nguyen, Hanh Tran Thi Thu, Tu Ly Anh, Tien Tran Van
The careful observation and evaluation of the squamocolumnar junction (SCJ) are essential requirements in colposcopy procedures. While traditional colposcopes with visible light offer high resolution and sensitivity, the presence of glare patterns on the cervix limits the visualization of deeper tissue features. Cross-polarization imaging, on the other hand, overcomes this limitation by effectively
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Distal-scanning common path probe for optical coherence tomography J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-02-09 Zhengyu Chen, Bin He, Zichen Yin, Zhangwei Hu, Yejiong Shi, Chengming Wang, Xiao Zhang, Ning Zhang, Linkai Jing, Guihuai Wang, Ping Xue
In this paper, we present a distal-scanning common path probe for optical coherence tomography (OCT) equipped with a hollow ultrasonic motor and a simple and specially designed beam-splitter. This novel probe proves to be able to effectively circumvent polarization and dispersion mismatch caused by fiber motion and is more robust to a variety of interfering factors during the imaging process, experimentally
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Vein visualization enhancement by dual-wavelength phase-locked denoising technology J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-01-29 Lihua Ruan, Zhiqin Yin, Shibing Zhou, Weibo Zheng, Wei Lu, Tao Zhang, Shaowei Wang
Visual near-infrared imaging equipment has broad applications in various fields such as venipuncture, facial injections, and safety verification due to its noncontact, compact, and portable design. Currently, most studies utilize near-infrared single-wavelength for image acquisition of veins. However, many substances in the skin, including water, protein, and melanin can create significant background
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A refined analytical model for reconstruction problems in diffuse reflectance spectroscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-01-20 Ekaterina Sergeeva, Daria Kurakina, Ilya Turchin, Mikhail Kirillin
A refined analytical model of spatially resolved diffuse reflectance with small source-detector separations (SDSs) for the in vivo skin studies is proposed. Compared to the conventional model developed by Farrell et al., it accounts for the limited acceptance angle of the detector fiber. The refined model is validated in the wide range of optical parameters by Monte Carlo simulations of skin diffuse
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Comparison of brain functions between healthy participants and methamphetamine users with various addiction histories: Data analysis based on EEG and fNIRS J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-01-20 Xuelin Gu, Xiaoou Li, Banghua Yang
The electroencephalogram (EEG) rhythm and functional near-infrared spectroscopy (fNIRS) activation levels have not been compared between a healthy control group (HCG) and methamphetamine user group (MUG) with different addiction histories. This study used 64-electrode EEG and fNIRS to conduct an experiment that analyzed the resting and craving states. The EEG and fNIRS data of 56 participants were
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Photoacoustic elastography based on laser-excited shear wave J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2024-01-12 Yang Liu, Ruoyi Shi, Gang Li, Mingjian Sun
Elastography can be used as a diagnostic method for quantitative characterization of tissue hardness information and thus, differential changes in pathophysiological states of tissues. In this study, we propose a new method for shear wave elastography (SWE) based on laser-excited shear wave, called photoacoustic shear wave elastography (PASWE), which combines photoacoustic (PA) technology with ultrafast
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Effect of Sodium Nitroprusside on the microrheological properties of red blood cells in different media J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-11-11 Petr Ermolinskiy, Matvey Maksimov, Andrei Lugovtsov, Alexey Muravyov, Irina Tikhomirova, Alexander Priezzhev
Red blood cell (RBC) aggregation as well as their deformation significantly affects blood microrheology. These processes depend on various factors, one of which is concentration of the nitric oxide, one of the main signaling molecule in the bloodstream. The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells (RBCs) in RBC samples
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Triple-path feature transform network for ring-array photoacoustic tomography image reconstruction J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-11-11 Lingyu Ma, Zezheng Qin, Yiming Ma, Mingjian Sun
Photoacoustic imaging (PAI) is a noninvasive emerging imaging method based on the photoacoustic effect, which provides necessary assistance for medical diagnosis. It has the characteristics of large imaging depth and high contrast. However, limited by the equipment cost and reconstruction time requirements, the existing PAI systems distributed with annular array transducers are difficult to take into
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Gates joint locally connected network for accurate and robust reconstruction in optical molecular tomography J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-11-04 Minghua Zhao, Yahui Xiao, Jiaqi Zhang, Xin Cao, Lin Wang
Optical molecular tomography (OMT) is a potential pre-clinical molecular imaging technique with applications in a variety of biomedical areas, which can provide non-invasive quantitative three-dimensional (3D) information regarding tumor distribution in living animals. The construction of optical transmission models and the application of reconstruction algorithms in traditional model-based reconstruction
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Deep learning-based inpainting of saturation artifacts in optical coherence tomography images J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-10-31 Muyun Hu, Zhuoqun Yuan, Di Yang, Jingzhu Zhao, Yanmei Liang
Limited by the dynamic range of the detector, saturation artifacts usually occur in optical coherence tomography (OCT) imaging for high scattering media. The available methods are difficult to remove saturation artifacts and restore texture completely in OCT images. We proposed a deep learning-based inpainting method of saturation artifacts in this paper. The generation mechanism of saturation artifacts
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Microwave-induced thermoacoustic elastic imaging: A simulation study J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-10-20 Lin Huang, Zheng Liang, Shuaiqi Qiao, Weipeng Wang
Microwave-induced thermoacoustic imaging (MTI) has the advantages of high resolution, high contrast, non-ionization, and non-invasive. Recently, MTI was used in the field of breast cancer screening. In this paper, based on the finite element method (FEM) and COMSOL Multiphysics software, a three-dimensional breast cancer model suitable for exploring the MTI process is proposed to investigate the influence
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MMAE-loaded PLGA nanomedicine with improved biosafety to achieve efficient antitumor treatment J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-10-07 Changqiang Xie, Yan Wang, Zhenzhen Cai, Jianghai Du, Zhengyu Chen, Junjie Wang, Xingzhou Peng
Monomethyl auristatin E (MMAE) is a derivative of the marine peptide Dolastatin 10, which has therapeutic effects against various cancers according to its antimitotic activity in multiple clinical trials. The antibody drug conjugate (ADC) of MMAE is currently used in clinical practice. However, the safety issues of MMAE-based ADC, such as high drug toxicity and poor bioavailability, still exist when
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Tissue optical clearing enhances efficacy of vascular targeted photodynamic therapy of mouse dorsal skin J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-08-30 Ying Liu, Qiushi Wang, Yidi Liu, Ying Wang, Haixia Qiu, Dan Zhu, Ying Gu, Defu Chen
Vascular-targeted photodynamic therapy (V-PDT) is an effective treatment for port wine stains (PWS). However, repeated treatment is usually needed to achieve optimal treatment outcomes, possibly due to the limited treatment light penetration depth in the PWS lesion. The optical clearing technique can increase light penetration in depth by reducing light scattering. This study aimed to investigate the
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Semantic segmentation of pyramidal neuron skeletons using geometric deep learning J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-08-23 Lanlan Li, Jing Qi, Yi Geng, Jingpeng Wu
Neurons can be abstractly represented as skeletons due to the filament nature of neurites. With the rapid development of imaging and image analysis techniques, an increasing amount of neuron skeleton data is being produced. In some scientific studies, it is necessary to dissect the axons and dendrites, which is typically done manually and is both tedious and time-consuming. To automate this process
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Three-dimensional morphological and fluorescent imaging of zebrafish with a continuous-rotational light-sheet microscope J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-08-19 Dongmin Zhang, Guang Yang, Yao Tan, Chong Chen, Jie Zhang, Hui Li
Light-sheet fluorescence microscopy (LSFM) has been widely used to image the three-dimensional (3D) structures and functions of various millimeter-size bio-specimen such as zebrafish. However, the sample adsorption and scattering cause shading of the light-sheet illumination, preventing the even 3D image of thick samples. Herein, we report a continuous-rotational light-sheet microscope (CR-LSM) that
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Estimation-free spatial-domain image reconstruction of structured illumination microscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-08-18 Xiaoyan Li, Shijie Tu, Yile Sun, Yubing Han, Xiang Hao, Cuifang kuang, Xu Liu
Structured illumination microscopy (SIM) achieves super-resolution (SR) by modulating the high-frequency information of the sample into the passband of the optical system and subsequent image reconstruction. The traditional Wiener-filtering-based reconstruction algorithm operates in the Fourier domain, it requires prior knowledge of the sinusoidal illumination patterns which makes the time-consuming
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Anti-PD1 antibody and not anti-LAG-3 antibody improves the antitumor effect of photodynamic therapy for treating metastatic breast cancer J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-08-05 Shan Long, Yibing Zhao, Yuanyuan Xu, Bo Wang, Haixia Qiu, Hongyou Zhao, Jing Zeng, Defu Chen, Hui Li, Jiakang Shao, Xiaosong Li, Ying Gu
Photodynamic therapy (PDT) has limited effects in treating metastatic breast cancer. Immune checkpoints can deplete the function of immune cells; however, the expression of immune checkpoints after PDT is unclear. This study investigates whether the limited efficacy of PDT is due to upregulated immune checkpoints and tries to combine the PDT and immune checkpoint inhibitor to observe the efficacy.
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The impact of central and obstructive respiratory events on cerebral oxygenation in adults with sleep disordered breathing J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-08-04 Ramin Khatami, Dominik Gnaiger, Gordana Hügli, Ming Qi, Zhongxing Zhang
Obstructive sleep apnea (OSA) and central sleep apnea (CSA) are two main types of sleep disordered breathing (SDB). While the changes in cerebral hemodynamics triggered by OSA events have been well studied using near-infrared spectroscopy (NIRS), they are essentially unknown in CSA in adults. Therefore, in this study, we compared the changes in cerebral oxygenation between OSA and CSA events in adult
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Automatic diagnosis of diabetic retinopathy using vision transformer based on wide-field optical coherence tomography angiography J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-07-29 Zenan Zhou, Huanhuan Yu, Jiaqing Zhao, Xiangning Wang, Qiang Wu, Cuixia Dai
Diabetic retinopathy (DR) is one of the major causes of visual impairment in adults with diabetes. Optical coherence tomography angiography (OCTA) is nowadays widely used as the golden criterion for diagnosing DR. Recently, wide-field OCTA (WF-OCTA) provided more abundant information including that of the peripheral retinal degenerative changes and it can contribute in accurately diagnosing DR. The
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Compact and robust dual-color linearly polarized illumination source for three-photon fluorescence imaging J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-07-22 Jiazheng Song, Yanyan Zhang, Yuanshan Liu
The miniaturized femtosecond laser in near infrared-II region is the core equipment of three-photon microscopy. In this paper, we design a compact and robust illumination source that emits dual-color linearly polarized light for three-photon microscopy. Based on an all-polarization-maintaining passive mode-locked fiber laser, we shift the center wavelength of the pulses to the 1.7μm band utilizing
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MACS-W: A modified optical clearing agent for imaging 3D cell cultures J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-07-12 Xiang Zhong, Chao Gao, Hui Li, Yuening He, Peng Fei, Zaozao Chen, Zhongze Gu, Dan Zhu, Tingting Yu
Three-dimensional (3D) cell cultures have contributed to a variety of biological research fields by filling the gap between monolayers and animal models. The modern optical sectioning microscopic methods make it possible to probe the complexity of 3D cell cultures but are limited by the inherent opaqueness. While tissue optical clearing methods have emerged as powerful tools for investigating whole-mount
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Optical manipulation of neuronal mitochondria using scanning optical tweezers J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-30 Zhiyong Gong, Tianli Wu, Xixi Chen, Ping Wang, Jinghui Guo, Yuchao Li, Baojun Li
Mitochondria play a crucial role in the physiological functions and energy metabolism of neurons, which can help in the understanding of complex biochemical reactions associated with various neurodegenerative diseases. Neurons, being highly differentiated terminal cells, require a greater number of mitochondria than ordinary cells to generate significant amounts of ATP, which is necessary for the growth
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A simple Bessel module with tunable focal depth and constant resolution for commercial two-photon microscope J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-30 Ting Mo, Yiran Liu, Fatao Bie, Zimin Dai, Jin Chang, Hui Gong, Wei Zhou
The volumetric imaging of two-photon microscopy expands the focal depth and improves the throughput, which has unparalleled superiority for three-dimension samples, especially in neuroscience. However, emerging in volumetric imaging is still largely customized, which limits the integration with commercial two-photon systems. Here, we analyzed the key parameters that modulate the focal depth and lateral
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Deep neural network based on multi-level wavelet and attention for structured illumination microscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-28 Yanwei Zhang, Song Lang, Xuan Cao, Hanqing Zheng, Yan Gong
Structured illumination microscopy (SIM) is a popular and powerful super-resolution (SR) technique in biomedical research. However, the conventional reconstruction algorithm for SIM heavily relies on the accurate prior knowledge of illumination patterns and signal-to-noise ratio (SNR) of raw images. To obtain high-quality SR images, several raw images need to be captured under high fluorescence level
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Intravascular photoacoustic and optical coherence tomography imaging dual-mode system for detecting spontaneous coronary artery dissection: A feasibility study J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-28 Yongwei Wang, Yuyang Wan, Zhongjiang Chen
In this work, we present an intravascular dual-mode endoscopic system capable of both intravascular photoacoustic imaging (IVPAI) and intravascular optical coherence tomography (IVOCT) for recognizing spontaneous coronary artery dissection (SCAD) phantoms. IVPAI provides high-resolution and high-penetration images of intramural hematoma (IMH) at different depths, so it is especially useful for imaging
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Motor network reorganization in stroke patients with dyskinesias during a shoulder-touching task: A fNIRS study J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-19 Yizheng Zhang, Dan Wang, Dongyang Wang, Kecheng Yan, Li Yi, Shuoshu Lin, Guangjian Shao, Zhiyong Shao, Jinyan Sun, Aoran Yang
Hemiplegia after stroke has become a major cause of the world’s high disabilities, and it is vital to enhance our understanding of post-stroke neuroplasticity to develop efficient rehabilitation programs. This study aimed to explore the brain activation and network reorganization of the motor cortex (MC) with functional near-infrared spectroscopy (fNIRS). The MC hemodynamic signals were gained from
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Artificial neural network-based determination of denoised optical properties in double integrating spheres measurement J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-14 Yusaku Takai, Takahiro Nishimura, Yu Shimojo, Kunio Awazu
Accurate determination of the optical properties of biological tissues enables quantitative understanding of light propagation in these tissues for optical diagnosis and treatment applications. The absorption (μa) and scattering (μs) coefficients of biological tissues are inversely analyzed from their diffuse reflectance (R) and total transmittance (T), which are measured using a double integrating
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Development of water-soluble AIE-based wash-free Aβ probes superior to commercial ThT J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-10 Ting-Ting Hou, Ying-Hao Tang, Zhen-Yu Zhang, Ze-Jun Li, Ya-Long Wang
Alzheimer’s disease (AD) is a typical neurodegenerative disease. β-amyloid (Aβ) plaque is the most prominent pathological biomarker associated with the progression of AD. Conventional Aβ probes, including commercial probe ThT, usually suffer from tedious washing procedures. Herein, novel AIE-active Aβ probes with excellent water solubility, named DE-V1-PYC3 and DE-V1-PYOH, were developed for the detection
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Laser speckle contrast imaging to predict the effect of temporary spinal cord stimulation in postherpetic neuralgia patients: A prospective observational study J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-06-10 Xiang Huang, Dacheng Tang, Pan Chen, Lumiao Chen, Xiaohong Li, Xiaoping Wang
Temporary spinal cord stimulation (tSCS) can effectively reduce the pain and severity of postherpetic neuralgia (PHN). However, there are no effective and objective methods for predicting the effects of tSCS on PHN. Laser speckle contrast imaging (LSCI) is frequently used in neurology to evaluate the effectiveness of treatment. To assess the accuracy of LSCI in predicting the impact of tSCS on PHN
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In vivo label-free measurement of blood flow velocity symmetry based on dual line scanning third-harmonic generation microscopy excited at the 1700 nm window J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-20 Hui Cheng, Jincheng Zhong, Ping Qiu, Ke Wang
Measurement of blood flow velocity is key to understanding physiology and pathology in vivo. While most measurements are performed at the middle of the blood vessel, little research has been done on characterizing the instantaneous blood flow velocity distribution. This is mainly due to the lack of measurement technology with high spatial and temporal resolution. Here, we tackle this problem with our
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Laser spectroscopy imaging technique coupled with nanomaterials for cancer diagnosis: A review J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-19 Zhengying Peng, Pengkun Yin, Dan Li, Youyuan Chen, Kaiqiang Shu, Qingwen Fan, Yixiang Duan, Qingyu Lin
Laser spectroscopic imaging techniques have received tremendous attention in the field of cancer diagnosis due to their high sensitivity, high temporal resolution, and short acquisition time. However, the limited tissue penetration of the laser is still a challenge for the in vivo diagnosis of deep-seated lesions. Nanomaterials have been universally integrated with spectroscopic imaging techniques
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Comparison of point detection and area detection for point-scanning structured illumination microscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-15 Wenshuai Wu, Jiajie Chen, Meiting Wang, Lei Wang, Xiaomin Zheng, Jia Li, Junle Qu, Bruce Zhi Gao, Yonghong Shao
Structured illumination microscopy (SIM) is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging speed. The system resolution is affected by two typical detection modes: Point detection and area detection. However, a systematic analysis of the imaging performance of the different detection modes of the system has rarely been conducted
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Cerebrovascular segmentation from mesoscopic optical images using Swin Transformer J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-11 Yuxin Li, Qianlong Zhang, Hang Zhou, Junhuai Li, Xiangning Li, Anan Li
Vascular segmentation is a crucial task in biomedical image processing, which is significant for analyzing and modeling vascular networks under physiological and pathological states. With advances in fluorescent labeling and mesoscopic optical techniques, it has become possible to map the whole-mouse-brain vascular networks at capillary resolution. However, segmenting vessels from mesoscopic optical
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Comparison of the emission wavelengths by a single fluorescent dye on in vivo 3-photon imaging of mouse brains J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-11 Ke Wang, Wanjian Zhang, Xiangquan Deng, Shen Tong, Hui Cheng, Mengyuan Qin, Lei Zheng, Kun Zhao, Ruizhan Zhai, Zhongqing Jia, Ping Qiu
Multiphoton microscopy (MPM) is a powerful imaging technology for brain research. The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels. It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases. However, there has been no comparison with near-infrared (NIR) emission. In order to quantitatively analyze
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Accuracy improvement for classifying retinal OCT images by diseases using deep learning-based selective denoising approach J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-11 Lantian Hu, Ruixiang Guo, Sifan Li, Jing Cao, Qian Liu
In ophthalmology, retinal optical coherence tomography (OCT) images with noticeable structural features help identify human eyes as healthy or diseased. The recently hot artificial intelligence (AI) realized this recognition process automatically. However, speckle noise in the original retinal OCT image reduces the accuracy of disease classification. This study presents a time-saving approach based
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Optical second-harmonic generation imaging for identifying gastrointestinal stromal tumors J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-05-06 Shichao Zhang, Xingxin Huang, Deyong Kang, Jikui Miao, Zhenlin Zhan, Guoxian Guan, Jianxin Chen, Yongjian Zhou, Lianhuang Li
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors arising in the digest tract. It brings a challenge to diagnosis because it is asymptomatic clinically. It is well known that tumor development is often accompanied by the changes in the morphology of collagen fibers. Nowadays, an emerging optical imaging technique, second-harmonic generation (SHG), can directly identify
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Immune modulations of the tumor microenvironment in response to phototherapy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-04-27 Trisha I. Valerio, Coline L. Furrer, Negar Sadeghipour, Sophia-Joy X. Patrock, Sayre A. Tillery, Ashley R. Hoover, Kaili Liu, Wei R. Chen
The tumor microenvironment (TME) promotes pro-tumor and anti-inflammatory metabolisms and suppresses the host immune system. It prevents immune cells from fighting against cancer effectively, resulting in limited efficacy of many current cancer treatment modalities. Different therapies aim to overcome the immunosuppressive TME by combining various approaches to synergize their effects for enhanced
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Three-dimensional structure of liver vessels and spatial distribution of hepatic immune cells J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-04-14 Mengli Xu, Zheng Liu, Xinlin Li, Xinru Wang, Xuenan Yuan, Chenlu Han, Zhihong Zhang
As the largest internal organ of the human body, the liver has an extremely complex vascular network and multiple types of immune cells. It plays an important role in blood circulation, material metabolism, and immune response. Optical imaging is an effective tool for studying fine vascular structure and immunocyte distribution of the liver. Here, we provide an overview of the structure and composition
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Detection of cells by flow cytometry: Counting, imaging, and cell classification J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-03-31 Yingsi Yu, Yimei Zheng, Caizhong Guan, Min Yi, Yunzhao Chen, Yaguang Zeng, Honglian Xiong, Xuehua Wang, Junping Zhong, Wenzheng Ding, Mingyi Wang, Xunbin Wei
The study of circulating cells in the blood stream is critical, as it covers many fields of biomedicine, including immunology, cell biology, oncology, and reproductive medicine. In-vivo flow cytometry (IVFC) is a new tool to monitor and count cells in real time for long durations in their native biological environment. This review describes two main categories of IVFC, i.e., labeled and label-free
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Transmissive multifocal laser speckle contrast imaging through thick tissue J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-03-17 Ruoyu Chen, Peng Miao, Shanbao Tong
Laser speckle contrast imaging (LSCI) is a powerful tool for monitoring blood flow changes in tissue or vessels in vivo, but its applications are limited by shallow penetration depth under reflective imaging configuration. The traditional LSCI setup has been used in transmissive imaging for depth extension up to 2lt–3lt (lt is the transport mean free path), but the blood flow estimation is biased due
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Comparison of uniform resampling and nonuniform sampling direct-reconstruction methods in k-space for FD-OCT J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-03-11 Yanrong Yang, Yun Dai, Yuehua Zhou, Yaliang Yang
The nonuniform distribution of interference spectrum in wavenumber k-space is a key issue to limit the imaging quality of Fourier-domain optical coherence tomography (FD-OCT). At present, the reconstruction quality at different depths among a variety of processing methods in k-space is still uncertain. Using simulated and experimental interference spectra at different depths, the effects of common
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DecodeSTORM: A user-friendly ImageJ plug-in for quantitative data analysis in single-molecule localization microscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-03-04 Qihang Song, Cheng Wu, Jianming Huang, Zhiwei Zhou, Zhen-Li Huang, Zhengxia Wang
Quantitative data analysis in single-molecule localization microscopy (SMLM) is crucial for studying cellular functions at the biomolecular level. In the past decade, several quantitative methods were developed for analyzing SMLM data; however, imaging artifacts in SMLM experiments reduce the accuracy of these methods, and these methods were seldom designed as user-friendly tools. Researchers are now
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Recent advances in Raman spectroscopy for skin diagnosis J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-03-04 Min Wu, Beibei Gao, Xunbin Wei
The skin is the largest organ in humans. It comprises about 16% of our body. Many diseases originate from the skin, including acne vulgaris, skin cancer, fungal skin disease, etc. As a common skin cancer in China, melanoma alone grows at year rate of nearly 4%. Therefore, it is crucial to develop an objective, reliable, accurate, non-invasive, and easy-to-use diagnostic method for skin diseases to
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Study on the secondary structure and hydration effect of human serum albumin under acidic pH and ethanol perturbation with IR/NIR spectroscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-28 Hui Zhang, Mengying Liang, Shuangshuang Li, Mengyin Tian, Xiaoying Wei, Bing Zhao, Haowei Wang, Qin Dong, Hengchang Zang
Human serum albumin (HSA) is the most abundant protein in plasma and plays an essential physiological role in the human body. Ethanol precipitation is the most widely used way to obtain HSA, and pH and ethanol are crucial factors affecting the process. In this study, infrared (IR) spectroscopy and near-infrared (NIR) spectroscopy in combination with chemometrics were used to investigate the changes
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Depolarizing metrics in the biomedical field: Vision enhancement and classification of biological tissues J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-23 Mónica Canabal-Carbia, Albert Van Eeckhout, Carla Rodríguez, Emilio González-Arnay, Irene Estévez, José J. Gil, Enrique García-Caurel, Razvigor Ossikovski, Juan Campos, Angel Lizana
Polarimetry encompasses a collection of optical techniques broadly used in a variety of fields. Nowadays, such techniques have provided their suitability in the biomedical field through the study of the polarimetric response of biological samples (retardance, dichroism and depolarization) by measuring certain polarimetric observables. One of these features, depolarization, is mainly produced by scattering
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Brain-wide N2cG compensation permits glycoprotein-deleted rabies virus to trace neural circuits across multiple synapses J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-22 Nengsong Luo, Zengpeng Han, Jiaxin Kou, Yuxiang Cai, Xin Yang, Jie Wang, Kunzhang Lin, Fuqiang Xu
Rabies-viruses-based retrograde tracers can spread across multiple synapses in a retrograde direction in the nervous system of rodents and primates, making them powerful tools for determining the structure and function of the complicated neural circuits of the brain. However, they have some limitations, such as posing high risks to human health and the inability to retrograde trans-synaptic label inputs
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Deep learning method for cell count from transmitted-light microscope J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-18 Mengyang Lu, Wei Shi, Zhengfen Jiang, Boyi Li, Dean Ta, Xin Liu
Automatic cell counting provides an effective tool for medical research and diagnosis. Currently, cell counting can be completed by transmitted-light microscope, however, it requires expert knowledge and the counting accuracy which is unsatisfied for overlapped cells. Further, the image-translation-based detection method has been proposed and the potential has been shown to accomplish cell counting
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High-throughput sorting of two-color fluorescent-labeled zebrafish embryos J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-16 Hongzhen Tang, Linbo Wang, Xiaohu Chen, Chong Chen, Hui Li, Guang Yang
The zebrafish embryos were widely employed in genetics, development and drug discovery studies as miniatured animal models. Sorting of two-color fluorescent embryos is often required in large-scale experiments but it is challenging to manually sort with high efficiency. Here, we reported a high-throughput sorting system for two-color fluorescent zebrafish embryos. The embryos can be automatically loaded
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SOFFLFM: Super-resolution optical fluctuation Fourier light-field microscopy J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-15 Haixin Huang, Haoyuan Qiu, Hanzhe Wu, Yihong Ji, Heng Li, Bin Yu, Danni Chen, Junle Qu
Fourier light-field microscopy (FLFM) uses a microlens array (MLA) to segment the Fourier plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional (3D) structure of the sample using 3D deconvolution calculation without scanning. However, the resolution of FLFM is still limited by diffraction, and furthermore, it is
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Photoacoustic microscopy based on transparent piezoelectric ultrasound transducers J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-11 Hangbing Peng, Zhongwen Cheng, Lvming Zeng, Xuanrong Ji
Photoacoustic microscopy (PAM), due to its deep penetration depth and high contrast, is playing an increasingly important role in biomedical imaging. PAM imaging systems equipped with conventional ultrasound transducers have demonstrated excellent imaging performance. However, these opaque ultrasonic transducers bring some constraints to the further development and application of PAM, such as complex
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Nonlinear optical microscopy for skin in vivo: Basics, development and applications J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-09 Jianhua Zhao, Yuan Zhao, Zhenguo Wu, Yunxian Tian, Haishan Zeng
Multi-photon microscopy (MPM) and coherent anti-Stokes Raman scattering (CARS) are two advanced nonlinear optical imaging techniques, which provide complementary information and have great potential in combination for noninvasive in vivo biomedical applications. This paper provides a detailed discussion of the basics, development and applications of these technologies for in vivo skin research, covering
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Fluorescence imaging analysis of the glioma microenvironment J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-06 Xuwen Peng, Yuzhou Chen, Yuke Wang, Shuhong Qi
Glioma is the most malignant brain cancer. The neurons, macrophages, T cells and other immune cells constitute the glioma immunosuppressive microenvironment. The accurate spatial distribution of these cells in the glioma microenvironment and its relationship with glioma metastasis is unknown. We constructed a mouse glioma cell line stably expressing the large Stokes-shifted yellow fluorescent protein
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A graphical user interface (GUI) for model-based radiation-induced acoustic computed tomography J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-06 Michelle Simon, Prabodh Kumar Pandey, Leshan Sun, Liangzhong Xiang
Radiation-induced acoustic computed tomography (RACT) is an evolving biomedical imaging modality that aims to reconstruct the radiation energy deposition in tissues. Traditional back-projection (BP) reconstructions carry noisy and limited-view artifacts. Model-based algorithms have been demonstrated to overcome the drawbacks of BPs. However, model-based algorithms are relatively more complex to develop
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Observing single cells in whole organs with optical imaging J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-02-04 Xiaoquan Yang, Tao Jiang, Lirui Liu, Xiaojun Zhao, Ximiao Yu, Minjun Yang, Guangcai Liu, Qingming Luo
Cells are the basic unit of human organs that are not fully understood. The revolutionary advancements of optical imaging allowed us to observe single cells in whole organs, revealing the complicated composition of cells with spatial information. Therefore, in this review, we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast
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A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device J. Innov. Opt. Health Sci. (IF 2.5) Pub Date : 2023-01-25 Qiushu Chen, Qi Meng, Yuzhe Liu, Xiangan Long, Yawei Kong, Longfang Yao, Liwen Chen, Chuanyong Wu, Kaiqin Chu, Lan Mi, Jiong Ma
Microfluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of-field (EDoF) phase modulator to the optical system, the entire depth of the microfluidic channel can