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VvWRKY5 positively regulates wounding-induced anthocyanin accumulation in grape by interplaying with VvMYBA1 and promoting jasmonic acid biosynthesis Hortic. Res. (IF 8.7) Pub Date : 2024-03-25 Zhen Zhang, Cui Chen, Changyue Jiang, Hong Lin, Yuhui Zhao, Yinshan Guo
Wounding stress induces the biosynthesis of various secondary metabolites in plants, including anthocyanin. However, the underlying molecular mechanism remains elusive. Here, we reported that a transcription factor, VvWRKY5, promotes wounding-induced anthocyanin accumulation in grape (Vitis vinifera). Biochemical and molecular analyses demonstrated that wounding stress significantly increased anthocyanin
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Calmodulin-like protein MdCML15 interacts with MdBT2 to modulate iron homeostasis in apple Hortic. Res. (IF 8.7) Pub Date : 2024-03-25 Xiao-Juan Liu, Xin Liu, Qiang Zhao, Yuan-Hua Dong, Qiangbo Liu, Yuan Xue, Yu-Xin Yao, Chun-Xiang You, Hui Kang, Xiao-Fei Wang
BTB and TAZ domain proteins (BTs) function as specialized adaptors facilitating substrate recognition of the CUL3-RING ubiquitin ligase (CRL3) complex that targets proteins for ubiquitination in reaction to diverse pressures. Nonetheless, knowledge of the molecular mechanisms by which the apple scaffold protein MdBT2 responds to external and internal signals is limited. Here, we demonstrate that a
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Genome-wide variants and optimal allelic combinations for citric acid in tomato Hortic. Res. (IF 8.7) Pub Date : 2024-03-18 Wenxian Gai, Liangdan Yuan, Fan Yang, John Kojo Ahiakpa, Fangman Li, Pingfei Ge, Xingyu Zhang, Jinbao Tao, Fei Wang, Yang Yang, Yuyang Zhang
Citric acid (CA) plays a crucial role as a fruit flavor enhancer and serves as a mediator in multiple metabolic pathways in tomato fruit development. Understanding factors influencing CA metabolism is essential for enhancing fruit flavor and CA-mediated biological processes. The accumulation of CA, however, is influenced by a complex interplay of genetic and environmental factors, leading to challenges
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MdbZIP44–MdCPRF2-like–Mdα-GP2 regulate starch and sugar metabolism in apple under nitrogen supply Hortic. Res. (IF 8.7) Pub Date : 2024-03-15 Xuejing Cao, Zhigang Guo, Ping Wang, Shixiong Lu, Wenfang Li, Zonghuan Ma, Juan Mao, Baihong Chen
Nitrogen (N) is regarded as an essential macronutrient and is tightly associated with carbon (C) metabolism in plants. The transcriptome data obtained from this study showed that the expression level of the apple basic leucine zipper (bZIP) transcription factor (TF) MdbZIP44 was up-regulated in ‘Oregon Spur Delicious’ (Malus domestica Borkh.) apple fruits under nitrogen supply. MdbZIP44 bound to the
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Diploid genome assembly of the Malbec grapevine cultivar enables haplotype-aware analysis of transcriptomic differences underlying clonal phenotypic variation Hortic. Res. (IF 8.7) Pub Date : 2024-03-14 Luciano Calderón, Pablo Carbonell-Bejerano, Claudio Muñoz, Laura Bree, Cristobal Sola, Daniel Bergamin, Walter Tulle, Sebastian Gomez-Talquenca, Christa Lanz, Carolina Royo, Javier Ibáñez, José Miguel Martinez-Zapater, Detlef Weigel, Diego Lijavetzky
To preserve their varietal attributes, established grapevine cultivars (Vitis vinifera L. ssp. vinifera) must be clonally propagated, due to their highly heterozygous genomes. Malbec is a France-originated cultivar appreciated for producing high-quality wines, and is the offspring of cultivars Prunelard and Magdeleine Noire des Charentes. Here, we have built a diploid genome assembly of Malbec, after
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Extracellular perception of multiple novel core effectors from the broad host-range pear anthracnose pathogen Colletotrichum fructicola in the nonhost Nicotiana benthamiana Hortic. Res. (IF 8.7) Pub Date : 2024-03-14 Mengqing Han, Chunhao Wang, Wenhui Zhu, Yuemin Pan, Lili Huang, Jiajun Nie
Colletotrichum fructicola is emerging as a devastating pathogenic fungus causing anthracnose in a wide range of horticultural crops, particularly fruits. Exploitation of nonhost resistance (NHR) represents a robust strategy for plant disease management. Perception of core effectors from phytopathogens frequently leads to hypersensitive cell death and resistance in nonhost plants, however, such core
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Chromosome-level genome assemblies of Musa ornata and M. velutina provide insights into pericarp dehiscence and anthocyanin biosynthesis in banana Hortic. Res. (IF 8.7) Pub Date : 2024-03-12 Tian-Wen Xiao, Xin Liu, Ning Fu, Tong-Jian Liu, Zheng-Feng Wang, Xue-Jun Ge, Hui-Run Huang
Musa ornata and M. velutina are members of the Musaceae family and are indigenous to the South and Southeast Asia. They are very popular in the horticultural market, but the lack of genomic sequencing data and genetic studies has hampered efforts to improve their ornamental value. In this study, we generated the first chromosome-level genome assemblies for both species by utilizing Oxford Nanopore
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Integrated proteomic, transcriptomic and metabolomic profiling reveals that GA-ABA hub runs the flower development in Chinese orchid Cymbidium sinense Hortic. Res. (IF 8.7) Pub Date : 2024-03-12 Sagheer Ahmad, Chuqiao Lu, Jie Gao, Yonglu Wei, Qi Xie, Jianpeng Jin, Genfa Zhu, Fengxi Yang
The seasonal flowering Chinese Cymbidium produce axillary floral meristem and require a dormancy period during cold conditions for flower development. However, their bud activation mechanism remains elusive. This study evaluates the multi-omics across six stages of flower development, along with functional analysis of core genes to decipher the innate mechanism of floral bud initiation and outgrowth
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MAPK20-mediated ATG6 phosphorylation is critical for pollen development in Solanum lycopersicum L. Hortic. Res. (IF 8.7) Pub Date : 2024-03-06 Yu Wang, Dongling Xie, Xuelian Zheng, Mingyue Guo, Zhenyu Qi, Ping Yang, Jingquan Yu, Jie Zhou
In flowering plants, male gametogenesis is tightly regulated by numerous genes. Mitogen-activated protein kinase (MAPK) plays a critical role in plant development and stress response, while its role in plant reproductive development is largely unclear. The present study demonstrated MAPK20 phosphorylation of ATG6 to mediate pollen development and germination in tomato (Solanum lycopersicum L.). MAPK20
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Two haplotype-resolved T2T reference genomes for Ziziphus jujuba and Z. jujuba var. spinosa provide new insights into the domestication of jujube Hortic. Res. (IF 8.7) Pub Date : 2024-03-04 Kun Li, Ruihong Chen, Ayimaiti Abudoukayoumu, Qian Wei, Zhibo Ma, Zhengyang Wang, Qing Hao, Jian Huang
Chinese jujube (Ziziphus jujuba Mill.) is one of the most important deciduous tree fruits in China, with substantial economic and nutritional value. Jujube was domesticated from its wild progenitor, wild jujube (Z. jujuba var. spinosa), with high medicinal value. Here, we report the 767.81Mb and 759.24 Mb haplotype-resolved assembly of a dry-eating ‘Junzao’ jujube (JZ) and a wild jujube accession (SZ)
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CsEXL3 regulate mechanical harvest-related droopy leaves under the transcriptional activation of CsBES1.2 in tea plant Hortic. Res. (IF 8.7) Pub Date : 2024-03-04 Haoran Liu, Lingxiao Duan, Jianqiang Ma, Jiqiang Jin, Rong Huang, Yujie Liu, Si Chen, Xiaoying Xu, Jiedan Chen, Mingzhe Yao, Liang Chen
Due to a labor shortage, the mechanical harvesting of tea plantations has become a focal point. However, mechanical harvest efficiency was hampered by droopy leaves, leading to a high rate of broken tea shoots and leaves. Here, we dissected the genetic structure of leaf droopiness in tea plants using genome-wide association studies (GWAS) on 146 accessions, combined with transcriptome from 2 accessions
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Multi-omics analysis reveals key regulatory defense pathways and genes involved in salt tolerance of rose plants Hortic. Res. (IF 8.7) Pub Date : 2024-03-02 Haoran Ren, Wenjing Yang, Weikun Jing, Muhammad Owais Shahid, Yuming Liu, Xianhan Qiu, Patrick Choisy, Tao Xu, Nan Ma, Junping Gao, Xiaofeng Zhou
Salinity stress causes serious damage to crops worldwide, limiting plant production. However, the metabolic and molecular mechanisms underlying the response to salt stress in rose (Rosa spp.) remain poorly studied. We therefore performed a multi-omics investigation of Rosa hybrida cv. Jardin de Granville (JDG) and Rosa damascena Mill. (DMS) under salt stress to determine the mechanisms underlying rose
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Chromosome-scale genome, together with transcriptome and metabolome, provided insights into the evolution and anthocyanin biosynthesis of Rubus rosaefolius Sm. (Rosaceae) Hortic. Res. (IF 8.7) Pub Date : 2024-03-02 Yunsheng Wang, Jiyuan Guan, Qunying Zhang
Rubus rosaefolius is a kind of red raspberry possessing high nutritional and pharmaceutical value. Here, we present a chromosome-level draft genome of R. rosaefolius. Of the total 131 assembled scaffolds, 70 with a total size of 219.02 Mb, accounting for 99.33% of the estimated genome size, were anchored to seven pseudochromosomes. We traced a whole-genome duplication (WGD) event shared among members
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Harnessing the power of microbes: Enhancing soybean growth in an acidic soil through AMF inoculation rather than P-fertilization Hortic. Res. (IF 8.7) Pub Date : 2024-03-02 Zhongling Wen, Minkai Yang, Aliya Fazal, Hongwei Han, Hongyan Lin, Tongming Yin, Yuelin Zhu, Shouping Yang, Kechang Niu, Shucun Sun, Jinliang Qi, Guihua Lu, Yonghua Yang
The low phosphorus (P) availability of acidic soils severely limits leguminous plant growth and productivity. Improving the soil P nutritional status can be achieved by increasing the P-content through P-fertilization or stimulating the mineralization of organic P via arbuscular mycorrhizal fungi (AMF) application, however, their corresponding impacts on plant and soil microbiome still remain to be
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CaSTH2 disables CaWRKY40 from activating pepper thermotolerance and immunity against Ralstonia solanacearum via physical interaction Hortic. Res. (IF 8.7) Pub Date : 2024-03-02 Xingge Cheng, Meiyun Wan, Yuqiu Song, Qian Liu, Xiaohui Hu, Xiufang Chen, Xujing Zhang, Yapeng Zhang, Ruijie Wu, Qiaoling Lu, Yu Huang, Jingang Lv, WeiWei Cai, Deyi Guan, Sheng Yang, Shuilin He
CaWRKY40 coordinately activates pepper immunity against Ralstonia solanacearum infection (RSI) and high temperature stress (HTS) forms positive feedback loops with other positive regulators and is promoted by CaWRKY27b/CaWRKY28 through physical interactions, however, whether and how it is regulated by negative regulators to function appropriately remain unclear. Herein, we provide evidence that CaWRKY40
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Pumpkin CmoDREB2A enhances salt tolerance of grafted cucumber through interaction with CmoNAC1 to regulate H2O2 and ABA signaling and K+/Na+ homeostasis Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Yuquan Peng, Lvjun Cui, Ying Wang, Lanxing Wei, Shouyu Geng, Hui Chen, Guoyu Chen, Li Yang, Zhilong Bie
Pumpkin CmoNAC1 enhances salt tolerance in grafted cucumbers. However, the potential interactions with other proteins that may co-regulate salt tolerance alongside CmoNAC1 have yet to be explored. In this study, we identified pumpkin CmoDREB2A as a pivotal transcription factor that interacts synergistically with CmoNAC1 in the co-regulation of salt tolerance. Both transcription factors were observed
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Phosphorylation of birch BpNAC90 improves the activation of gene expression to confer drought tolerance Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Zhibo Wang, Zihang He, Caiqiu Gao, Chao Wang, Xingshun Song, Yucheng Wang
The NAC transcription factors (TFs) play important roles in mediating abiotic stress tolerance; however, the mechanism is still not fully known. Here, an NAC gene (BpNAC90) from a gene regulatory network of Betula platyphylla (birch) that responded to drought was characterized. Overexpression and knockout of BpNAC90 displayed increased and reduced drought tolerance, respectively, relative to wild-type
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Dissection of the spatial dynamics of biosynthesis, transport, and turnover of major amino acids in tea plants (Camellia sinensis) Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Shuwei Yu, Mingzhi Zhu, Ping Li, Hao Zuo, Juan Li, Yingying Li, Anqi Peng, Jianan Huang, Alisdair R Fernie, Zhonghua Liu, Jian Zhao
High levels of free amino acids (AAs) in tea leaves are crucial for tea flavor and health function, however, the dynamic AA biosynthesis, transport and turnover in tea plants remain elusive. Here we dissected whole tea plants for these dynamics by assessing AA profiles and transcriptomes of metabolic pathway genes in tea roots, stems, and leaves and revealing their distinctive features with regard
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Ethylene-responsive VviERF003 modulates glycosylated monoterpenoid synthesis by upregulating VviGT14 in grapes Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Ya-Chen Wang, Yi Wei, Xiang-Yi Li, Hui-Min Zhang, Xiao Meng, Chang-Qing Duan, Qiu-Hong Pan
Terpenoids are important contributors to the aroma of grapes and wines. Grapes contain terpenoids in both volatile free form and non-volatile glycosidic form, with the latter being more abundant. Glycosylated terpenoids are deemed as latent aromatic potentials for their essential role in adding to the flowery and fruity bouquet of wines. However, transcriptional regulatory mechanism underlying glycosylated
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Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 XiaoXue Sun, Zihan Liu, Rui Liu, Johan Bucher, Jianjun Zhao, Richard G F Visser, Guusje Bonnema
In Chinese cabbage, rosette leaves expose their adaxial side to the light converting light energy into chemical energy, acting as a source for the growth of the leafy head. In the leafy head, the outer heading leaves expose their abaxial side to the light while the inner leaves are shielded from the light and have become a sink organ of the growing Chinese cabbage plant. Interestingly, variation in
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RNA sequencing analysis reveals PgbHLH28 as the key regulator in response to methyl jasmonate-induced saponin accumulation in Platycodon grandiflorus Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Wuhua Zhang, Jinzhu Zhang, Yingdong Fan, Jie Dong, Peng Gao, Wanzheng Jiang, Tao Yang, Daidi Che
Platycodon grandiflorus (Jacq.) A. DC, known for its saponin content, can potentially prevent and treat cerebrovascular diseases and COVID-19. Triterpenoid saponin biosynthesis in plants is enhanced by methyl jasmonate (MeJA) application. However, the underlying molecular mechanisms of MeJA-induced saponin biosynthesis remain unknown in P. grandiflorus. In the current study, exogenous application of
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Chromosome-scale reference genome of broccoli (Brassica oleracea var. italica Plenck) provides insights into glucosinolate biosynthesis Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Qiuyun Wu, Shuxiang Mao, Huiping Huang, Juan Liu, Xuan Chen, Linghui Hou, Yuxiao Tian, Jiahui Zhang, Junwei Wang, Yunsheng Wang, Ke Huang
Broccoli (Brassica oleracea var. italica Plenck) is an important vegetable crop, as it is rich in health-beneficial glucosinolates (GSLs). However, the genetic basis of the GSL diversity in Brassicaceae remains unclear. Here, we report a chromosome-level genome assembly of broccoli generated using PacBio HiFi reads and Hi-C technology. The final genome assembly is 613.79 Mb in size, with a contig N50
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RNA-seq-based comparative transcriptome analysis reveals the role of CsPrx73 in waterlogging triggered adventitious root formation in cucumber Hortic. Res. (IF 8.7) Pub Date : 2024-02-29 Jiawei Pan, Jia Song, Hamza Sohail, Rahat Sharif, Wenjing Yan, Qiming Hu, Xiaohua Qi, Xiaodong Yang, Xuewen Xu, Xuehao Chen
Abiotic stressors like waterlogging are detrimental to cucumber development and growth. However, comprehension of the highly complex molecular mechanism underlying waterlogging can provide an opportunity to enhance cucumber tolerance under waterlogging stress. We examined the hypocotyl and stage-specific transcriptomes of the waterlogging-tolerant YZ026A and the waterlogging-sensitive YZ106A, which
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Diversity of flavour characteristics of table grapes and their contributing volatile compounds analysed by the solvent-assisted flavour evaporation method Hortic. Res. (IF 8.7) Pub Date : 2024-02-26 Kazuki Moriyama, Atsushi Kono, Ryusuke Matsuzaki, Akifumi Azuma, Noriyuki Onoue, Yoshihiko Sekozawa, Akihiko Sato, Sumiko Sugaya
To identify the compounds that contribute to the diverse flavours of table grapes, the flavours and volatile compounds of 38 grape cultivars harvested over three years are evaluated through sensory analysis and solvent-assisted flavour evaporation (SAFE). The cultivars are characterised and grouped into seven clusters by hierarchical cluster analysis (HCA) using sensory evaluation data with a flavour
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MdARF3 switches the lateral root elongation to regulate dwarfing in apple plants Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Jiahong Lv, Yi Feng, Longmei Zhai, Lizhong Jiang, Yue Wu, Yimei Huang, Runqi Yu, Ting Wu, Xinzhong Zhang, Yi Wang, Zhenhai Han
Apple rootstock dwarfing and dense planting are common practices in apple farming. However, the dwarfing mechanisms are not understood. In this study, the expression of MdARF3 in the root system of dwarfing rootstock 'M9' was lower than in the vigorous rootstock from Malus micromalus due to the deletion of the WUSATAg element in the promoter of the 'M9' genotype. Notably, this deletion variation was
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Defensive alteration of root exudate composition by grafting Prunus sp. onto resistant rootstock contributes to reducing crown gall disease Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Lin Chen, Lusen Bian, Qinghua Ma, Ying Li, Xinghong Wang, Yunpeng Liu
Grafting is a traditional and significant strategy to suppress soilborne diseases, such as the crown gall disease caused by tumorigenic Agrobacterium and Rhizobium. Root exudates and the rhizosphere microbiome play critical roles in controlling crown gall disease, but their role in suppressing crown gall disease by grafted plants remains unclear. Here, disease-susceptible cherry rootstock “Gisela 6”
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The succinct title: Genome-wide methylation, transcriptome and characteristic metabolites reveal the balance between diosgenin and brassinosteroids in Dioscorea zingiberensis Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Zihao Li, Yi Li, Luyu Geng, Jiachen Wang, Yidan Ouyang, Jiaru Li
Diosgenin (DG) is a bioactive metabolite isolated from Dioscorea species, renowned for its medicinal properties. Brassinosteroids (BRs) are a class of crucial plant steroidal hormones. Cholesterol and campesterol are important intermediates of DG and BRs biosynthesis, respectively. DG and BRs are structurally similar components, however, the regulatory network and metabolic interplays have not been
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Banana MabHLH28 positively regulates the expression of softening-related genes to mediate fruit ripening independently or via cooperating with MaWRKY49/111 Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Chaojie Wu, Danling Cai, Jun Li, Zengxiang Lin, Wei Wei, Wei Shan, Jianye Chen, Wangjin Lu, Xinguo Su, Jianfei Kuang
Texture softening is a physiological indicator of fruit ripening, which eventually contributes to fruit quality and consumer’s acceptance. Despite great progress has been made in identification of the genes related to fruit softening, the upstream transcriptional regulatory pathways of these softening-related genes are not fully elucidated. Here, a novel bHLH gene, designated as MabHLH28, was identified
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PsmiR159b-PsMYB65 module functions in the resumption of bud growth after endodormancy by affecting the cell cycle in tree peony Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Tao Zhang, Xinyu Wang, Yanchao Yuan, Shoujie Zhu, Chunying Liu, Yuxi Zhang, Shupeng Gai
Bud endodormancy in perennial plants is a sophisticated system that adapts to seasonal climatic changes. Growth-promoting signals such as low temperature and gibberellins (GAs) are crucial for facilitating budbreak following endodormancy release (EDR). However, the regulatory mechanisms underlying GA-mediated budbreak in tree peony (Paeonia suffruticosa) remain unclear. In tree peony, the expression
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Emerging roles and mechanisms of lncRNAs in fruit and vegetables Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Xiuming Zhao, Fujun Li, Maratab Ali, Xiaoan Li, Xiaodong Fu, Xinhua Zhang
With the development of genome sequencing technologies, many long non-coding RNAs (lncRNAs) have been identified in fruit and vegetables. LncRNAs are primarily transcribed and spliced by RNA polymerase II (Pol II) or plant-specific Pol IV/V, and exhibit limited evolutionary conservation. LncRNAs intricately regulate various aspects of fruit and vegetables, including pigment accumulation, reproductive
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A mutation in the brassinosteroid biosynthesis gene CpDWF5 disrupts vegetative and reproductive development and salt stress response in squash (Cucurbita pepo) Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Sonsoles Alonso, Gustavo Cebrián, Keshav Gautam, Jessica Iglesias-Moya, Cecilia Martínez, Manuel Jamilena
A Cucurbita pepo EMS mutant with multiple defects in growth and development has been identified and characterized. The mutant dwfcp displayed a dwarf phenotype with dark green and shrinking leaves, shortened internodes and petioles, shorter but thicker roots and greater root biomass, and reduced fertility. The causal mutation of the phenotype was found to disrupt gene Cp4.1LG17g04540, the squash orthologue
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The Smi–miR858a-SmMYB module regulates tanshinone and phenolic acid biosynthesis in Salvia miltiorrhiza Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Butuo Zhu, Meizhen Wang, Yongqi Pang, Xiangling Hu, Chao Sun, Hong Zhou, Yuxing Deng, Shanfa Lu
Tanshinones and phenolic acids are two major classes of bioactive compounds in S. miltiorrhiza. Revealing the regulatory mechanism of their biosynthesis is crucial for quality improvement of S. miltiorrhiza medicinal materials. Here we demonstrated that Smi-miR858a–Smi-miR858c, a miRNA family previously known to regulate flavonoid biosynthesis, also played critical regulatory roles in tanshinone and
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Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Yin Xin, Xi Chen, Jiahui Liang, Shaokun Wang, Wenqiang Pan, Jingxiang Wu, Mingfang Zhang, Michele Zaccai, Xiaonan Yu, Xiuhai Zhang, Jian Wu, Yunpeng Du
Lily bulbils, which serve as advantageous axillary organs for vegetative propagation, have not been extensively studied in terms of the mechanism of bulbil initiation. The function of auxin and sucrose metabolism have been implicated in axillary organ development, but their relationship in regulating bulbil initiation remains unclear. In this study, exogenous indole-3-acetic acid (IAA) treatment increased
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A natural variation in SlSCaBP8 promoter contributes to the loss of saline-alkaline tolerance during tomato improvement Hortic. Res. (IF 8.7) Pub Date : 2024-02-23 Jian Liu, Chi Zhang, Heyao Sun, Yinqiang Zang, Xianwen Meng, Huawei Zhai, Qian Chen, Chuanyou Li
Saline-alkaline stress is a worldwide problem that threatens the growth and yield of crops. However, how crops adapt to saline-alkaline stress remains less studied. Here we show that the saline-alkaline tolerance was compromised during tomato domestication and improvement, and a natural variation in promoter of SlSCaBP8, an EF-hand Ca2+ binding protein, contributes to the loss of saline-alkaline tolerance
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cla-miR164-NO APICAL MERISTEM (ClNAM) Regulates the Inflorescence Architecture Development of Chrysanthemum lavandulifolium Hortic. Res. (IF 8.7) Pub Date : 2024-02-19 Junzhuo Li, Xiaohui Wen, Qiuling Zhang, Yuankai Tian, Ya Pu, Jiaying Wang, Bo Liu, Yihan Du, Silan Dai
Chrysanthemum ×morifolium has great ornamental and economic value on account of its exquisite capitulum. However, previous studies have mainly focused on the corolla morphology of the capitulum. Such an approach cannot explain the variable inflorescence architecture of the chrysanthemum. Previous research from our group has shown that NO APICAL MERISTEM (ClNAM) is likely to function as a hub gene in
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TSPO-induced degradation of the ethylene receptor RhETR3 promotes salt tolerance in rose (Rosa hybrida) Hortic. Res. (IF 8.7) Pub Date : 2024-02-17 Qingcui Zhao, Weikun Jing, Xijia Fu, Ruoyun Yang, Chunyan Zhu, Jiaxin Zhao, Patrick Choisy, Tao Xu, Nan Ma, Liangjun Zhao, Junping Gao, Xiaofeng Zhou, Yonghong Li
The gaseous plant hormone ethylene regulates plant development, growth, and responses to stress. In particular, ethylene affects tolerance to salinity; however, the underlying mechanisms of ethylene signaling and salt tolerance are not fully understood. Here, we demonstrate that salt stress induces the degradation of the ethylene receptor ETHYLENE RESPONSE 3 (RhETR3) in rose (Rosa hybrid). Furthermore
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Hypermethylation in the promoter regions of flavonoid pathway genes is associated with skin color fading during ‘Daihong’ apple fruit development Hortic. Res. (IF 8.7) Pub Date : 2024-02-17 Jihua Xu, Lan Xiong, Jia-Long Yao, Peilei Zhao, Shenghui Jiang, Xiaohong Sun, Chaohua Dong, Hongyuan Jiang, Xinyue Xu, Yugang Zhang
Apple fruit skin color fading is not well understood although the molecular mechanism of skin color formation is well known. The red-fleshed apple cultivar ‘Daihong’ (‘DH’) exhibited fading skin color during fruit development despite having a heterozygous R6 allele but lacking Red-TE for red fruit skin. In this study, transcriptomic analysis revealed the expression level of MdMYB10 increased with fruit
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Transcriptional regulation of flavonol biosynthesis in plants Hortic. Res. (IF 8.7) Pub Date : 2024-02-17 Yunlin Cao, Yuyang Mei, Ruining Zhang, Zelong Zhong, Xiaochun Yang, Changjie Xu, Kunsong Chen, Xian Li
Summary Flavonols are a class of flavonoids that play a crucial role in regulating plant growth and promoting stress resistance. They are also important dietary components in horticultural crops due to their benefits for human health. In past decades, research on the transcriptional regulation of flavonol biosynthesis in plants has increased rapidly. This review summarized recent progress in the flavonol-specific
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LaMYC7, a positive regulator of linalool and caryophyllene biosynthesis, confers plant resistance to Pseudomonas syringae Hortic. Res. (IF 8.7) Pub Date : 2024-02-13 Yanmei Dong, Ziling Wei, Wenying Zhang, Jingrui Li, Meixian Han, Hongtong Bai, Hui Li, Lei Shi
Linalool and caryophyllene are the main monoterpene and sesquiterpene compounds in lavender; however, the genes regulating their biosynthesis still remain many unknowns. Here, we identified LaMYC7, a positive regulator of linalool and caryophyllene biosynthesis, that confers plant resistance to Pseudomonas syringae. LaMYC7 was highly expressed in glandular trichomes, and LaMYC7 overexpression could
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The chromosome-scale genome and population genomics reveal the adaptative evolution of Populus pruinosa to desertification environment Hortic. Res. (IF 8.7) Pub Date : 2024-02-06 Jianhao Sun, Jindong Xu, Chen Qiu, Juntuan Zhai, Shanhe Zhang, Xiao Zhang, Zhihua Wu, Zhijun Li
The Populus pruinosa is a relic plant that has managed to survive in extremely harsh desert environments. Owing to intensifying global warming and desertification, research into ecological adaptation and speciation of P. pruinosa have attracted considerable interest, but the lack of a chromosome-scale genome has limited adaptive evolution research. Here, a 521.09 Mb chromosome-level reference genome
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Genome-wide identification of bHLH transcription factors and functional analysis in salt gland development of the recretohalophyte sea lavender (Limonium bicolor) Hortic. Res. (IF 8.7) Pub Date : 2024-02-02 Xi Wang, Baoshan Wang, Fang Yuan
Transcription factors with basic helix–loop–helix (bHLH) structures regulate plant growth, epidermal structure development, metabolic processes, and responses to stress extensively. Sea lavender (Limonium bicolor) is a recretohalophyte with unique salt glands in the epidermis that make it highly resistant to salt stress, and contribute to improve saline lands. However, the features of the bHLH transcription
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The Cissus quadrangularis genome reveals its adaptive features in arid habitat Hortic. Res. (IF 8.7) Pub Date : 2024-02-02 Qingyun Li, Yi Wang, Huimin Zhou, Yuanshuang Liu, Duncan Kiragu Gichuki, Yujun Hou, Jisen Zhang, Rishi Aryal, Guangwan Hu, Tao Wan, Sara Getachew Amenu, Robert Wahiti Gituru, Haiping Xin, Qingfeng Wang
Cissus quadrangularis is a tetraploid species belonging to the Vitaceae family and is known for the Crassulacean acid metabolism (CAM) pathway in the succulent stem, while the leaves perform C3 photosynthesis. Here, we report a high-quality genome of C. quadrangularis comprising a total size of 679.2Mb which was phased into two subgenomes. Genome annotation identified 51,857 protein-coding genes, while
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Identification and application of an exocarp-preferential promoter for genetic engineering of tomato fruit Hortic. Res. (IF 8.7) Pub Date : 2024-02-01 Xue-Ming Ruan, Xiangyu Xiong, Jian-Feng Li
Tomato (Solanum lycopersicum) is a globally cultivated crop with great economic value. The exocarp determines the appearance of tomato fruit and protects it from various biotic and abiotic challenges at both pre-harvest and post-harvest stages. However, no tomato exocarp-specific promoter is currently available, which hinders exocarp-based genetic engineering. Here, we identified by RNA sequencing
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T2T Reference Genome Assembly and Genome-Wide Association Study Reveal the Genetic Basis of Chinese bayberry Fruit Quality Hortic. Res. (IF 8.7) Pub Date : 2024-01-31 Shuwen Zhang, Zheping Yu, Li Sun, Senmiao Liang, Fei Xu, Sujuan Li, Xiliang Zheng, Lijv Yan, Yinghong Huang, Xingjiang Qi, Haiying Ren
Chinese bayberry (Myrica rubra or Morella rubra; 2n=16) produces fruit with a distinctive flavor, high nutritional, and economic value. However, previous versions of the bayberry genome lack sequence continuity. Moreover, to date, no large-scale germplasm resource association analysis has examined the allelic and genetic variations determining fruit quality traits. Therefore, in this study, we assembled
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Lipidomics, transcription analysis, and hormone profiling unveil the role of CsLOX6 in MeJA biosynthesis during black tea processing Hortic. Res. (IF 8.7) Pub Date : 2024-01-31 Gaoyang Zhang, Jingjing Wei, Linyan Li, Dandan Cui
Jasmonates, such as jasmonic acid (JA) and methyl jasmonate (MeJA), are crucial aspect of black tea quality. However, lipids species, hormones and genes regulated mechanism in the jasmonate biosynthesis during black tea processing are lacking. In this study, we employed lipidomics, hormone metabolism analysis, and transcriptome profiling of genes associated with the MeJA biosynthesis pathway to investigate
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Apple E3 ligase MdPUB23 mediates ubiquitin-dependent degradation of MdABI5 to delay ABA-triggered leaf senescence Hortic. Res. (IF 8.7) Pub Date : 2024-01-31 Fei Yang, Ling-Ling Zhao, Lai-Qing Song, Yuepeng Han, Chun-Xiang You, Jian-Ping An
ABSCISIC ACID-INSENSITIVE5 (ABI5) is a core regulatory factor that mediates the ABA signaling response and leaf senescence. However, the molecular mechanism underlying the synergistic regulation of leaf senescence by ABI5 with interacting partners and the homeostasis of ABI5 in the ABA signaling response remain to be further investigated. In this study, we found that the accelerated effect of MdABI5
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Two large inversions seriously suppress recombination and are essential for key genotype fixation in cabbage (Brassica oleracea L. var. capitata) Hortic. Res. (IF 8.7) Pub Date : 2024-01-31 Bin Zhang, Yuankang Wu, Shoufan Li, Limei Yang, Mu Zhuang, Honghao Lv, Yong Wang, Jialei Ji, Xilin Hou, Fengqing Han, Yangyong Zhang
Chromosomal inversion is an important structural variation that usually suppresses recombination and is critical for key genotype fixation. In a previous study, an 11.47 Mb recombination suppression region was identified in the yellow–green leaf locus BoYgl-1 on chromosome 1, but the cause of recombination suppression is still unclear. In this study, chlorophyll and carotenoid contents were found to
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Allele-specific DNA methylation and gene expression during shoot organogenesis in tissue culture of hybrid poplar Hortic. Res. (IF 8.7) Pub Date : 2024-01-25 Ying Guo, Yang-Fan Feng, Gang-Gui Yang, Yan Jia, Jie He, Ze-Yu Wu, Hao-Ran Liao, Qi-Xuan Wei, Liang-Jiao Xue
Plant tissue regeneration is critical for genetic transformation and genome editing techniques. During the regeneration process, changes in epigenetic modifications accompany the cell fate transition. However, how allele-specific DNA methylation in two haplotypes contributes to the transcriptional dynamics during regeneration remains elusive. Here, we applied an inter-species hybrid poplar (Populus
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PMAT: an efficient plant mitogenome assembly toolkit using low coverage HiFi sequencing data Hortic. Res. (IF 8.7) Pub Date : 2024-01-25 Changwei Bi, Fei Shen, Fuchuan Han, Yanshu Qu, Jing Hou, Kewang Xu, Li-an Xu, Wenchuang He, Zhiqiang Wu, Tongming Yin
Complete mitochondrial genome (mitogenome) of plants are valuable resources for nucleocytoplasmic interactions, plant evolution, and plant cytoplasmic male sterile line breeding. However, the complete assembly of plant mitogenomes is challenging due to frequent recombination events and horizontal gene transfers. Previous studies have adopted Illumina, PacBio, and Nanopore sequencing data to assemble
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SlWRKY80-mediated JA pathway positively regulates tomato resistance to saline-alkali stress by enhancing spermidine content and stabilizing Na+/K+ homeostasis Hortic. Res. (IF 8.7) Pub Date : 2024-01-25 Chunyu Shang, Xiaoyan Liu, Guo Chen, Hao Zheng, Abid Khan, Guobin Li, Xiaohui Hu
Saline-alkali is one important abiotic stressor influencing tomato production. Exogenous MeJA is well known to increase tomato resistance to a variety of stresses, although its exact mechanism is yet unknown. In this study, we confirmed that 22.5 μmol/L MeJA could significantly improve the saline-alkali stress resistance of tomato. 300 mM saline-alkali stress increased the endogenous MeJA and JA content
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5-Aminolevulinic acid improves cold resistance through regulation of SlMYB4/SlMYB88-SlGSTU43 module to scavenge reactive oxygen species in tomato Hortic. Res. (IF 8.7) Pub Date : 2024-01-21 Zhengda Zhang, Luqiao Yuan, Jiao Dang, Yuhui Zhang, Yongshuai Wen, Yu Du, Yufei Liang, Ya Wang, Tao Liu, Tianlai Li, Xiaohui Hu
Cold stress severely affects the growth and quality of tomato. 5-Aminolevulinic acid (ALA) can effectively improve tomato's cold stress tolerance. In this study, a tomato glutathione S-transferase gene, SlGSTU43, was identified. Results showed that ALA strongly induced the expression of SlGSTU43 under cold stress. SlGSTU43-overexpressing lines showed increased resistance to cold stress through an enhanced
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A haplotype-resolved gap-free genome assembly provides novel insight into monoterpenoid diversification in Mentha suaveolens ‘Variegata’ Hortic. Res. (IF 8.7) Pub Date : 2024-01-18 Hanting Yang, Can Wang, Guanru Zhou, Yuxuan Zhang, Tianxing He, Lulu Yang, Ya Wu, Zhengnan Wang, Xin Tang, Gang Chen, Zhaoyu Liu, Huanyu Tang, Hanlin Zhou, Xumei Kang, Sanyin Zhang, Liang Leng, Shilin Chen, Chi Song
Mentha is a commonly used spice worldwide, which possesses medicinal properties and fragrance. These characteristics are conferred, at least partially, by essential oils such as menthol. In this study, a gap-free assembly with a genome size of 414.3 Mb and 31,251 coding genes was obtained for Mentha suaveolens ‘Variegata’. Based on its high heterozygosity (1.5%), two complete haplotypic assemblies
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Leguminous green manure intercropping changes the soil microbial community and increases soil nutrients and key quality components of tea leaves Hortic. Res. (IF 8.7) Pub Date : 2024-01-18 Yu Duan, Ting Wang, Xiaogang Lei, Yu Cao, Lefeng Liu, Zhongwei Zou, Yuanchun Ma, Xujun Zhu, Wanping Fang
Intercropping, a green and sustainable planting pattern, has demonstrated positive effects on plant growth and soil environment. However, there is currently a little research on the influence of intercropping leguminous plants and using them as green manure on soil environment and tea quality. During profuse flowering period of Chinese milkvetch, the contents of tea amino acids and soluble sugar in
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Genetic regulation of volatile production in two melon introgression line collections with contrasting ripening behavior Hortic. Res. (IF 8.7) Pub Date : 2024-01-17 Carlos Mayobre, Miguel Santo Domingo, Elif Nur Özkan, Andrés Fernández-Borbolla, Javier Ruiz-Lasierra, Jordi Garcia-Mas, Marta Pujol
The importance of melon aroma in determining fruit quality has been highlighted in recent years. The fruit volatile profile is influenced by the type of fruit ripening. Non-climacteric fruits contain predominantly aldehydes, while climacteric fruits mainly produce esters. Several genes have been described to participate in volatile organic compounds (VOCs) biosynthesis pathways, but knowledge in this
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BL-hi-C reveals the 3D genome structure of brassica crops with high sensitivity Hortic. Res. (IF 8.7) Pub Date : 2024-01-17 Lupeng Zhang, Ranze Zhao, Jianli Liang, Xu Cai, Lei Zhang, Huiling Guo, Zhicheng Zhang, Jian Wu, Xiaowu Wang
High-throughput Chromatin Conformation Capture (Hi-C) technologies can be used to investigate the three-dimensional genomic structure of plants. However, the practical utility of these technologies is impeded by significant background noise, hindering their capability in detecting fine 3D genomic structures. In this study, we optimized the Bridge Linker Hi-C technology (BL-Hi-C) to comprehensively
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Insights into the functional role of tomato TM6 as transcriptional regulator of flower development Hortic. Res. (IF 8.7) Pub Date : 2024-01-17 Rocío Fonseca, Carmen Capel, Ricardo Lebrón, Ana Ortiz-Atienza, Fernando J Yuste-Lisbona, Trinidad Angosto, Juan Capel, Rafael Lozano
Flower development is a crucial step towards the completion of plant life cycle. Physiological processes and gene regulatory mechanisms underlying flower formation have been extensively characterized, and the implication of MADS-box transcription factors as primary regulators of flower morphology has been widely described, mainly due to the analysis of loss-of-function mutants in model species. Nevertheless
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Enhanced photosynthetic efficiency by nitrogen-doped carbon dots via plastoquinone-involved electron transfer in plants Hortic. Res. (IF 8.7) Pub Date : 2024-01-12 Xiuli Jing, Yankai Liu, Xuzhe Liu, Yi Zhang, Guanzhu Wang, Fei Yang, Yani Zhang, Dayong Chang, Zhen-Lu Zhang, Chun-Xiang You, Shuai Zhang, Xiao-Fei Wang
Artificially enhancing photosynthesis is critical for improving crop yields and fruit qualities. Nanomaterials have demonstrated great potential to enhance photosynthetic efficiency, however, the mechanisms underlying their effects are poorly understood. This study revealed that the electron transfer pathway participated in nitrogen-doped carbon dots (N-CDs)-induced photosynthetic efficiency enhancement
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A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato Hortic. Res. (IF 8.7) Pub Date : 2024-01-12 Sulaiman Ahmed, Muhammad Saad Shoaib Khan, Songlei Xue, Faisal Islam, Aziz Ul Ikram, Muhammad Abdullah, Shan Liu, Piengtawan Tappiban, Jian Chen
Biotic and abiotic stresses negatively affect the yield and overall plant developmental process, thus causing substantial losses in global sweet potato production. To cope with stresses, sweet potato has evolved numerous strategies to tackle ever-changing surroundings and biological and environmental conditions. The invention of modern sequencing technology and the latest data processing and analysis
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Genome and GWAS analysis identified genes significantly related to phenotypic state of rhododendron bark Hortic. Res. (IF 8.7) Pub Date : 2024-01-11 Qiannan Ye, Lu Zhang, Qing Li, Yaliang Ji, Yanli Zhou, Zhenzhen Wu, Yanting Hu, Yongpeng Ma, Jihua Wang, Chengjun Zhang
As an important horticultural plant, Rhododendron is often used in urban greening and landscape design. However, factors such as the high rate of genetic recombination, frequent outcrossing in the wild, weak linkage disequilibrium, and the susceptibility of gene expression to environmental factors limit further exploration of functional genes related to important horticultural traits, and making the
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Towards the completion of Koch’s postulates for the citrus huanglongbing bacterium, Candidatus Liberibacter asiaticus Hortic. Res. (IF 8.7) Pub Date : 2024-01-11 Desen Zheng, Cheryl Armstrong, Wei Yao, Bo Wu, Weiqi Luo, Charles Powell, Wayne Hunter, Feng Luo, Dean Gabriel, Yongping Duan
Candidatus Liberibacter asiaticus (Las) is one of the causal agents of huanglongbing (HLB), the most devastating disease of citrus worldwide. Due to the intracellular lifestyle and significant genome reduction, culturing Las in vitro has proven to be extremely challenging. In this study, we optimized growth conditions and developed a semi-selective medium based on the results of nutritional and antibiotic