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Diagnostic insights from chemometric analysis of hemogram inflammatory indexes in male smokers with and without asthma or chronic obstructive pulmonary disease Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-16 Brajesh Singh, Vijay Dutta, Smiti Singh, Priyanka Pundit
BackgroundDiagnosis of asthma and chronic obstructive pulmonary disease (COPD) becomes difficult in a primary healthcare center due to ambiguous interpretation of spirometry and lack of facility to access established biomarkers. While routine hematological indices are easily available and accessible. The study aimed to evaluate the role of different hemogram indexes in males in COPD, asthma, and healthy
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Schistocyte detection in artificial intelligence age Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-13 Zeng Zhang, Su Yang, Xiuhong Wang
Schistocytes are fragmented red blood cells produced as a result of mechanical damage to erythrocytes, usually due to microangiopathic thrombotic diseases or mechanical factors. The early laboratory detection of schistocytes has a critical impact on the timely diagnosis, effective treatment, and positive prognosis of diseases such as thrombocytopenic purpura and hemolytic uremic syndrome. Due to the
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Neonatal diagnosis of congenital dyserythropoietic anemia type II Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-13 Apolline Lelard, Baptiste Le Calvez, Simon Bouzy, Marie Laure Couec, Marion Eveillard
CONFLICT OF INTEREST STATEMENT The authors have no conflict of interest to disclose.
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Merkel cell carcinoma mimicking acute leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-13 Jonathan Keow, Keith F. Kwan, Ben D. Hedley, Cyrus C. Hsia, Anargyros Xenocostas, Benjamin Chin‐Yee
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How to investigate mild to moderate bleeding disorders and bleeding disorder of unknown cause Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-08 Alessandro Casini, Johanna Gebhart
A bleeding tendency is one of the most common complaints observed by hematologists. It is challenging to differentiate a clinically insignificant bleeding from a bleeding phenotype that requires hemostatic evaluation and medical intervention. A thorough review of personal and familial history, objective assessment of bleeding severity using a bleeding assessment tool, and a focused physical examination
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Immunophenotypic characterization of leukemic stem cells in acute myeloid leukemia using single tube 10‐colour panel by multiparametric flow cytometry: Deciphering the spectrum, complexity and immunophenotypic heterogeneity Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-08 Nupur Das, Devasis Panda, Smeeta Gajendra, Ritu Gupta, Deepshi Thakral, Gurvinder Kaur, Aafreen Khan, Vivek Kumar Singh, Arushi Vemprala, Sameer Bakhshi, Rachna Seth, Ranjit Kumar Sahoo, Atul Sharma, Sandeep Rai, Vijay K. Prajapati, Saroj Singh
IntroductionDespite extensive research, comprehensive characterization of leukaemic stem cells (LSC) and information on their immunophenotypic differences from normal haematopoietic stem cells (HSC) is lacking. Herein, we attempted to unravel the immunophenotypic (IPT) characteristics and heterogeneity of LSC using multiparametric flow cytometry (MFC) and single‐cell sequencing.Materials and MethodsBone
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Identification of a novel ITGAX::RN7SL2 fusion in acute myelocytic leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-07 Zhan Su, Beibei Cong, Xiaojuan Li
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White blood cells scattergram as a valuable tool for COVID‐19 screening: A multicentric study Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-05 Jennifer Osman, Cécile Gonnin, Jérome Lambert, Céline Behier, Nicolas Chapuis, Simon Chevalier, Jérôme Debus, Anne Delaval, Maxime Depoorter, Cécile Dumas, Amély Dumesges, Pascale Dussert, Corinne Ferrero Vacher, Frédérique Dubois‐Galopin, Delphine Gerard, Pauline Gravière Bollotte, Geoffroy Guignedoux, Caroline Mayeur‐Rousse, Delphine Mercier‐Bataille, Emily Ronez, Catherine Trichet, Margaux Wiber
IntroductionNew tools have been developed to distinguish the COVID‐19 diagnosis from other viral infections presenting similar symptomatology and mitigate the lack of sensitivity of molecular testing. We previously identified a specific “sandglass” aspect on the white blood cells (WBC) scattergram of COVID‐19 patients, as a highly reliable COVID‐19 screening test (sensitivity: 85.9%, specificity: 83
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A career in solving clinical-pathological conundrums: Heyde syndrome, anti-platelet factor 4 disorders, and microvascular limb ischemic necrosis Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-03 Theodore E. Warkentin
Hematology is a clinical specialty with strong roots in the laboratory; accordingly, the lab can help solve perplexing clinical problems. This review highlights clinical-pathological conundrums addressed during my 35-year hematology career at McMaster University. Heyde syndrome is the association between aortic stenosis and bleeding gastrointestinal (GI) angiodysplasia where the bleeding is usually
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Variability in combinations of APTT reagent and substrate plasma for a one‐stage clotting assay to measure factor VIII products Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-03-01 Atsuo Suzuki, Nobuaki Suzuki, Takeshi Kanematsu, Shuichi Okamoto, Naruko Suzuki, Shogo Tamura, Ryosuke Kikuchi, Akira Katsumi, Tetsuhito Kojima, Tadashi Matsushita
IntroductionAn investigation of the suitability of reagents for measuring FVIII products in a one‐stage clotting assay (OSA) showed variations in their FVIII activity (FVIII:C). Most studies have focused on the activated partial thromboplastin time (APTT) reagent rather than FVIII‐deficient plasma (F8DP), even though the APTT‐based OSA is comprised of APTT reagents and factor‐deficient plasma.AimA
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Evaluation of AQUIOS STEM, a novel method for automated CD34+ stem cell enumeration using flow cytometry Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-29 Kim Callebaut, Eva Gijbels, Jonathan Vanbesien, Dries Deeren, Jan Van Droogenbroeck, Jan Emmerechts, Elisabeth Moreau
ObjectivesHematopoietic stem cell transplantation (HSCT) is a potentially curative treatment for various diseases. The measurement of CD34+ cells is crucial to schedule the peripheral blood stem cell collection and assess the engraftment potential of the apheresis product. The AQUIOS STEM system has been introduced as a novel application on the AQUIOS CL, a fully automated flow cytometer, for the enumeration
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Neutrophil to lymphocyte ratio and systemic immune‐inflammatory index as markers of response to autologous hematopoietic stem cell transplantation in persons with multiple sclerosis Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-29 Guillermo Ocaña‐Ramm, Moisés Manuel Gallardo‐Pérez, Solón Javier Garcés‐Eisele, Daniela Sánchez‐Bonilla, Max Robles‐Nasta, Edgar Jared Hernández‐Flores, Luis Enrique Hamilton‐Avilés, Paola Negrete‐Rodríguez, Miranda Melgar‐de‐la‐Paz, Olivia Lira‐Lara, Juan Carlos Olivares‐Gazca, Guillermo J. Ruiz‐Delgado, Guillermo J. Ruiz‐Argüelles
IntroductionBiomarkers that help to evaluate the immune system and could be useful in multiple sclerosis (MS) are the neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR), and systemic immune‐inflammatory index (SII). The objective of this work is to evaluate the significance of the SII index, PLR, and NLR before and after transplantation in individuals with MS who underwent autologous
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Blood smear invaders: About a fatal case of bacteremia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-27 Aurélien Bauduin, Marine Demoy, Franck Genevieve, Marie Tuffigo, Damien Luque Paz, Agathe Boussaroque
CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest.
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PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay: A highly sensitive method for detection of MYD88 L265P mutation Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-23 Marcello Viscovo, Mia de Laurent Clemmensen, Federica Fosso, Elena Maiolo, Francesco Autore, Luca Laurenti, Stefan Hohaus, Patrizia Chiusolo
IntroductionAgarose gel‐based conventional and real‐time allele‐specific polymerase chain reaction (AS‐PCR) assays are currently used for sensitive detection and quantification of MYD88 L265P mutation. Visual inspection of an agarose gel can often be ambiguous. We propose a new allele‐specific quantification PCR (AS‐qPCR) assay, PlentiPlex™ MYD88 Waldenström lymphoma qPCR assay, that uses Intercalating
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Identification and clinical implications of recurrent PAX5::MLLT3 fusion in lymphoid blastic phase chronic myeloid leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-23 Lijun Wang, Yu Chen, Qingyuan Wang, Meng Xiang, Zhao Zeng, Zhibo Zhang, Fenghong Zhang, Suning Chen, Mengxing Xue
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The lupus anticoagulant titer is associated with elevated antiphosphatidylserine/prothrombin immunoglobulin‐M isotype antibody levels Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-21 Abdulrahman Saadalla, Dong Chen, Melissa Stuart, Rajiv Pruthi, Nahla Heikal, Melissa Snyder, Aneel Ashrani, Jansen Seheult
BackgroundClot based assays used for lupus anticoagulant (LAC) detection are typically interpreted in a qualitative fashion and may not reflect LAC potency. In this cross‐sectional study, we describe a method for quantifying the LAC titer using serial (dependent) two‐fold dilutions in normal pooled plasma.MethodsSerial dilutions of 51 residual plasma samples from 50 patients were tested using the Russell's
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Imaging flow cytometric detection of del(17p) in bone marrow and circulating plasma cells in multiple myeloma Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-21 Thomas I. Mincherton, Stephanie J. Lam, Sarah E. Clarke, Henry Y. L. Hui, Jacques A. J. Malherbe, Hun S. Chuah, M. Hasib Sidiqi, Kathy A. Fuller, Wendy N. Erber
BackgroundDetection of del(17p) in myeloma is generally performed by fluorescence in situ hybridization (FISH) on a slide with analysis of up to 200 nuclei. The small cell sample analyzed makes this a low precision test. We report the utility of an automated FISH method, called “immuno‐flowFISH”, to detect plasma cells with adverse prognostic risk del(17p) in bone marrow and blood samples of patients
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Transient red blood cell agglutination after Magrolimab administration in acute myeloid leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-17 Antoine Leveque, Delphine Rolland, Marie-Pierre Ledoux, Célestine Simand, Alice Eischen, Caroline Mayeur-Rousse
CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest.
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Prognostic significance of bone marrow fibrosis in diffuse large B-cell lymphoma Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-15 Tugba Cetintepe, Gamze Ozkan, Betul Polat Kucukzeybek, Lutfi Cetintepe, Demet Kiper Unal, Serife Solmaz, Kemal Aygun, Alev Garip Acar, Sadi Bener, Aylin Orgen Çallı, Kadriye Bahriye Payzın
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphomas. The aim of this study is to determine the relationship between the increase in the degree of fibrosis in the bone marrow and prognosis and mortality in newly diagnosed DLBCL.
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Role of miRNAs in T-cell activation and Th17/Treg-cell imbalance in acquired aplastic anemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-15 G. Sabreen, Khaliqur Rahman, Ruchi Gupta, Chandra P. Chaturvedi, Jyotika Srivastava, Dinesh Chandra, Manish K. Singh, S. Yadav, Akhilesh Sharma, Manoj Sarkar, Rajesh Kashyap
Altered T-cell repertoire with an aberrant T-cell activation and imbalance of the Th17/Treg cells has been reported in acquired aplastic anemia (aAA). miRNAs are well known to orchestrate T-cell activation and differentiation, however, their role in aAA is poorly characterized. The study aimed at identifying the profile of miRNAs likely to be involved in T-cell activation and the Th17/Treg-cell imbalance
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A circulating proplatelet identified in an iron‐deficient patient Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-19 Helbies Bedier, Jessica Theriault, David M. Conrad
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Digital morphology compared to the optical microscope: A validation study on reporting bone marrow aspirates Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-08 G. Zini, P. Chiusolo, E. Rossi, E. Di Stasio, S. Bellesi, T. Za, M. Viscovo, F. Frioni, F. Ramundo, N. Pelliccioni, V. De Stefano
This study aims to evaluate the effectiveness and reliability of the utilization for clinical reporting of the evaluation of digital images of bone marrow aspirates by morphologists and their comparability with the classic microscopic morphological evaluation.
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Accuracy of automated analyzers for the estimation of CSF cell counts: A systematic review and meta-analysis Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-07 Greer E. Waldrop, Kaitlyn Cocuzzo, Colleen L. Schneider, Carla Y. Kim, Teddy G. Goetz, Mashina S. Chomba, Clare E. Delaurentis, Marie C. Smithgall, Richard O. Francis, Kiran T. Thakur
This systematic review evaluates the evidence for accuracy of automated analyzers that estimate cerebrospinal fluid (CSF) white blood cell counts (WBC) compared to manual microscopy. Inclusion criteria of original research articles included human subjects, English language, and manual microscopy comparator. PUBMED, EMBASE and Cochrane Review databases were searched through 2019 and QUADAS-2 Tool was
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The effect of oscillation depolymerization on ethylenediaminetetraacetic acid-dependent platelet aggregation samples: A cross-over study Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-07 Dan Liu, Wei Yang
Ethylenediaminetetraacetic acid (EDTA)-dependent platelet aggregation (PA) can cause medical errors. Currently, there is no reliable method for completely solving this problem. This study aims to solve this problem that has plagued clinical practice for many years by using oscillation method.
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Whole blood clotting time: Current practices among Thailand National External Quality Assessment Scheme for blood coagulation member laboratories Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-06 Preechaya Wongkrajang, Wimol Chinswangwatanakul, Panutsaya Tientadakul, Achara Chantarat
The Thai National Guidelines for Hemostatic Laboratory Testing were established in 2018. The guidelines recommend that the 20-min whole blood clotting time (20WBCT) method be used to diagnose/monitor snake bites. The aim of this study was to survey members of the Thailand National External Quality Assessment Scheme (NEQAS) for Blood Coagulation to investigate the use of 20WBCT testing compared between
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Taipan snake venom time has high sensitivity for lupus anticoagulants in non-anticoagulated, triple positive antiphospholipid syndrome patients Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-01 Gary W. Moore, Sean Platton, Nada Yartey, Eleanor Foxton, Danielle White, Stephen G. MacDonald
Dilute Russell's viper venom time (dRVVT) and activated partial thromboplastin time (APTT) are the mainstay assays in lupus anticoagulant (LA) detection yet they have limitations, particularly in relation to interferences and specificity. The recently validated Taipan snake venom time (TSVT) screening with ecarin time (ET) confirmatory assays overcome many of those limitations due to the innate specificity
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Solitary central nervous system relapse in acute promyelocytic leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-02-01 Truong Pham Hong Diem, Yu-Chen Su, Mei-Yu Su, Chieh-Lin Jerry Teng
CONFLICT OF INTEREST STATEMENT Chieh-Lin Jerry Teng received honorarium and consulting fees from Novartis, Roche, Pfizer, Takeda, Johnson and Johnson, Amgen, BMS Celgene, Kirin, and MSD. The other authors have no conflicts of interest.
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Hemostatic imbalance induced by tamoxifen in estrogen receptor-positive breast cancer patients: An observational study Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-31 Marie Didembourg, Sara Reda, Johannes Oldenburg, Heiko Rühl, Jonathan Douxfils, Laure Morimont
Estrogen receptor (ER)-positive (ER+) breast cancer accounts for approximately 75% of all breast cancers. Tamoxifen, a selective estrogen receptor modulator, is the standard adjuvant treatment. Although better tolerated than aromatase inhibitors, tamoxifen increases the risk of venous thromboembolism (VTE) 1.4-fold.
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Relapsed B-cell prolymphocytic leukemia (B-PLL) with distinctive granular inclusion bodies Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-29 Mei-Yu Su, Tsung-Chih Chen, Chieh-Lin Jerry Teng, Cheng-Han Wu
CONFLICT OF INTEREST STATEMENT Chieh-Lin Jerry Teng received an honorarium and consulting fees from Novartis, Roche, Takeda, Johnson & Johnson, Amgen, BMS Celgene, Kirin, AbbVie, and MSD. The other authors declare that they have no conflicts of interest.
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Use of clinical biological tests of haemostasis to evaluate topical haemostatics Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-29 Fabien Nativel, Sophie Tollec, Kamel-Olivier Sellal, Marc Trossaërt, Gaël Grimandi
In addition to traditional means, topical haemostatics are currently used to avoid haemorrhage during surgery. Although they have been reported to be effective, there is a low level of proof of their clinical efficacy, which is at odds with their levels of use. This study used two methods to better understand their in vitro mechanism of action.
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Validation of a novel algorithm with a high specificity in ruling out MDS Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-29 Felicitas Schulz, Kathrin Nachtkamp, Howard S. Oster, Moshe Mittelman, Norbert Gattermann, Sarah Schweier, Carmen Barthuber, Ulrich Germing
A previously published web-based App using Gradient-boosted models (GBMs) of eight laboratory parameters was established by Oster et al. to facilitate diagnosis or exclusion of myelodysplastic syndromes (MDS) in patients.
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Acute myeloid leukemia with misleading cytology Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-23 Mayssa Gaaloul, Madalina Uzunov, Karim Maloum, Elise Sourdeau
A 78-year-old woman presented with asthenia and superficial bleeding. Blood tests revealed pancytopenia with neutropenia (0.38 × 109/L), anemia (94 g/L), and thrombocytopenia (50 × 109/L). The peripheral blood smear showed 9% immature hypergranular atypical cells morphologically looking like abnormal promyelocytes and frequently containing bundles of Auer rods (Figure 1A–C). No schistocytes were present
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Reference intervals of cell population data parameters in Sysmex XN-Series and its patterns of changes from early adulthood to geriatric ages in South Korea Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-23 Yong Jun Choi, Ju-Heon Park, Seon Cho, Hyeran Park, Suyoung Kim, Eunjoo Kwon, Han-Ik Cho, Eun-Hee Nah
Cell population data (CPD) parameters may be putative biomarkers for the screening of various diseases including some infections and myelodysplastic syndrome. This study aimed to establish the age- and sex-specific reference intervals (RIs) for the CPD parameters in the Korean population.
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Primary plasma cell leukemia presented with atypical flower-like morphology Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-20 Ljubomir Jakovic, Jelica Jovanovic, Nada Kraguljac Kurtovic, Marija Dencic Fekete, Andrija Bogdanovic
CONFLICT OF INTEREST STATEMENT The authors declare no conflicts of interest.
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ICSH review of internal quality control policy for blood cell counters Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-12 Richard McCafferty, George Cembrowski, Barbara de la Salle, Mingting Peng, Eloisa Urrechaga
This paper is a report of an ICSH review of policies and practices for internal quality control (IQC) policy for haematology cell counters among regulatory bodies, cell counter manufacturers and diagnostic laboratories. It includes a discussion of the study findings and links to separate ICSH guidance for such policies and practices. The application of internal quality control (IQC) methods is an essential
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Performance comparison of two automated digital morphology analyzers for leukocyte differential in patients with malignant hematological diseases: Mindray MC-80 and Sysmex DI-60 Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-11 Xianfei Ye, Lijuan Fang, Yunying Chen, Jixiang Tong, Xiaoni Ning, Lanjun Feng, Yuting Xu, Dagan Yang
The MC-80 (Mindray, Shenzhen, China), a newly available artificial intelligence (AI)-based digital morphology analyzer, is the focus of this study. We aim to compare the leukocyte differential performance of the Mindray MC-80 with that of the Sysmex DI-60 and the gold standard, manual microscopy.
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ICSH guidance for internal quality control policy for blood cell counters Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-08 Richard McCafferty, George Cembrowski, Barbara de la Salle, Mingting Peng, Eloisa Urrechaga
This paper is a description of the ICSH guidance for internal quality control (IQC) policy for blood cell counters. It follows from and links to a separate ICSH review for such policies and practices. The ICSH has gathered information regarding the current state of practice through review of published guidance from regulatory bodies, a questionnaire to six major cell counter manufacturers and a survey
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False positive lupus anticoagulant tests in patients with high C-reactive protein: A comparison of two hexagonal phase reagents Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-07 Ryan Mize, Sarah Mueller, Maxwell Roth, Gabriella Montgomery, Elisabeth M. Battinelli, Sacha Uljon
Lupus anticoagulants (LA) are a heterogeneous group of autoantibodies that bind to negatively charged phospholipids or protein/phospholipid complexes. The design of a laboratory testing algorithm is complicated by the biological heterogeneity of LA and by the variable sensitivity, specificity, and patterns of interference for different reagents. Three expert groups have published recommendations on
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SARS-CoV-2 infection is not associated with the emergence of monoclonal gammopathies Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-07 Cecilia Simonini, Patrizia Natali, Tommaso Pirotti, Vincenzo Nasillo, Giovanni Riva, Johanna Chester, Tommaso Trenti, Daria Debbia
Upon infection activated plasma cells produce large quantities of antibodies which can lead to the emergence of a monoclonal component (MC), detectable by serum protein electrophoresis (SPEP). This study aims to investigate any correlation between SARS-CoV-2 infection and MC development and, if identified, whether it persists during follow-up.
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Clinicopathologic features of relapsed CD19(−) B-ALL in CD19-targeted immunotherapy: Biological insights into relapse and LILRB1 as a novel B-cell marker for CD19(−) B lymphoblasts Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2024-01-04 Dong Chen, Franklin Fuda, Flavia Rosado, Sílvia Saumell, Samuel John, Mingyi Chen, Prasad Koduru, Weina Chen
The mechanism of relapsed CD19(−) B-ALL after anti-CD19 immunotherapy (Kymriah [CART-19] and blinatumomab) is under active investigation. Our study aims to assess LILRB1 as a novel B-cell marker for detecting CD19(−) B-lymphoblasts and to analyze the clinicopathologic/genetic features of such disease to provide biological insight into relapse.
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Evaluation of a diagnostic platelet aggregation test strategy for platelet rich plasma samples with low platelet counts Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-26 Rahaf Mahmoud Altahan, Natalie Mathews, Alex Bourguignon, Subia Tasneem, Donald M. Arnold, Wendy Lim, Catherine P. M. Hayward
Light transmission aggregometry (LTA) is important for diagnosing platelet function disorders (PFD) and von Willebrand disease (VWD) affecting ristocetin-induced platelet aggregation (RIPA). Nonetheless, data is lacking on the utility of LTA for investigating thrombocytopenic patients and platelet rich plasma samples with low platelet counts (L-PRP). Previously, we developed a strategy for diagnostic
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Metrological traceability in flow cytometry? Evaluation of a new volumetric method for lymphocyte subsets Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-19 Rosalie Y. Ward, Matthew Stevens, Sheriaz Bashir
Lymphocyte subset enumeration by flow cytometry is important for the therapeutic monitoring of a range of conditions. However, current bead-based methodologies do not produce metrologically traceable results. Here we compare an established bead-based methodology with a volumetric-based system traceable to an internationally recognised reference method.
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Platelet-to-lymphocyte ratio and absolute monocyte count have prognostic potential in primary myelodysplastic neoplasms Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-17 Huiting Qu, Jingxue Chu, Lu Wang, Jie Zhang, Jingyin Han, Zhen Li, Haifeng Hou, Yong Wang, Yigang Liu, Huanling Wu
The platelet-to-lymphocyte ratio (PLR), peripheral blood absolute monocyte count (AMC), and monocyte-to-lymphocyte ratio (MLR) are considered biomarkers of systemic immune and inflammation response. However, their prognostic potential in patients with myelodysplastic neoplasms (MDS) remains unclear. This study aimed to explore the predictive impact of PLR, MLR, and AMC on MDS outcomes.
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MicroRNA-based therapies: Revolutionizing the treatment of acute myeloid leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-17 Radhakrishnan Chandraprabha Vineetha, John Anitha Geetha Raj, Padmakumar Devipriya, Mohanan Sreelatha Mahitha, Sreedharan Hariharan
MicroRNAs (miRNAs) are small noncoding epigenetic regulators that exert critical significance by influencing target mRNAs and governing gene expression patterns and cellular signaling pathways. miRNAs play a pivotal role in a wide array of biological processes, including cell differentiation, proliferation, and survival. Numerous miRNAs contribute to tumorigenesis and cancer progression by promoting
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Detection of dysplasia in peripheral blood: Proposal of an algorithm to detect myelodysplastic syndromes and chronic myelomonocytic leukemias on a high-speed technical platform using the Sysmex XN™ analyser Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-11 Lakhdar Bouriche, Léa Fuster, Hugo Laurent, Christophe Soler, Sofiane Benhabib
Chronic Myelomonocytic Leukemia (CMML) and Myelodysplastic Syndromes (MDS) are increasingly represented in the general population. We propose a screening strategy based on algorithms calculated from quantitative and analytical data from the XN analyser.
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Prognostic significance of the frequencies of bone marrow lymphocyte subsets in adult acute myeloid leukemia at diagnosis Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-09 Kai Sun, Jun Wang, Ya-Zhe Wang, Zong-Yan Shi, Yan Chang, Xiao-Ying Yuan, Yan-Rong Liu, Hao Jiang, Qian Jiang, Xiao-Jun Huang, Ya-Zhen Qin
Immune microenvironment plays an important role in the occurrence and development of acute myeloid leukemia (AML). Studies assessing the prognostic significance of bone marrow (BM) lymphocyte subsets' frequencies at diagnosis in patients with AML were limited.
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FLAER as a standalone reagent for paroxysmal nocturnal hemoglobinuria: Do we need to reconsider the guidelines for testing? Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-09 Praveen Sharma, Parveen Bose, Nabhajit Mallik, Dikshat Gopal Gupta, Suneel Rachagiri, Arun Kumar, Jasbir Kaur, Pankaj Malhotra, Neelam Varma, Man Updesh Singh Sachdeva
Flow cytometry-based paroxysmal nocturnal hemoglobinuria (PNH) testing involves utilization of monoclonal antibodies against GPI-linked proteins and FLAER. The ability of FLAER to bind to a wide variety of GPI-linked structures and to be utilized across different leukocyte subsets is remarkable. We hypothesize that FLAER as a standalone reagent may be equally effective for detecting PNH clones. The
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Th9/IL-9 may participate in the pathogenesis of multiple myeloma Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-06 Yihan Liu, Yinghua Xie, Xiyao Han, Pei Li, Jianan Zhou, Xinxin Hu, Qiuyun Wang
This research is aimed to evaluate the correlation between Th9-associated cytokine levels in MM patients, clinical features, and therapy.
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Report of the International Council for Standardization in Haematology working group for standardization of reticulocyte parameters Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-06 Amrom E. Obstfeld, Bruce H. Davis, Jin-Yeong Han, Eloisa Urrechaga
The International Council for Standardization in Haematology convened a working group to assess and propose improvements upon the state of standardization and harmonization of reticulocyte parameters among commercial hematology analyzers.
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Thrombin generation assay in platelet-poor plasma in children with iron deficiency anemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-12-01 Umur Özdöl, Zeynep Canan Özdemir, Ersin Töret, Hülya Özen, Özcan Bör
Iron deficiency anemia (IDA) is the most common type of anemia in childhood and it leads to a hypercoagulable state. We investigated endogenous thrombin production in platelet-poor plasma before and after oral iron replacement in children with IDA using the thrombin generation assay (TGA).
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Anti-factor Xa as the preferred assay to monitor heparin for the treatment of pulmonary embolism Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-21 Eric Zhu, Eugene Yuriditsky, Veronica Raco, Alyson Katz, John Papadopoulos, James Horowitz, Thomas Maldonado, Tania Ahuja
The mainstay of acute pulmonary embolism (PE) treatment is anticoagulation. Timely anticoagulation correlates with decreased PE-associated mortality, but the ability to achieve a therapeutic activated partial thromboplastin time (aPTT) with unfractionated heparin (UFH) remains limited. Although some institutions have switched to a more accurate and reproducible test to assess for heparin's effectiveness
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Reference intervals and biological variation in parameters of the thrombin generation test in healthy individuals Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-20 Melnichnikova Olga, Zhilenkova Yuliya, Lukinov Vitaly, Zolotova Ekaterina, Pishchulov Konstantin, Evgina Svetlana, Simakova Maria, Vavilova Tatyana
Establish the referenceintervals (RIs) and analyze biological variability (BV) to introduce the thrombin generation test (TGT) into clinical practice.
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International council for standardisation in haematology recommendations on fibrinogen assays, thrombin clotting time and related tests in the investigation of bleeding disorders Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-20 Ian Mackie, Alessandro Casini, Marlien Pieters, Rajiv Pruthi, Chris Reilly-Stitt, Atsuo Suzuki
This guidance was prepared on behalf of the International Council for Standardisation in Haematology (ICSH) by an international working group of clinicians and scientists. The document focuses on tests and assays used for the assessment of fibrinogen function, particularly in the scenario of bleeding disorders. Thrombin clotting time (TT) is used as a screening test in some laboratories and also has
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Neutrophil Reactivity Intensity and misclassification of immature granulocytes Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-20 Elias Frost Wiwe, Isabella Katarina Kofoed, Muriel Julie Falk Dufréchou, Jens Peter Philipsen, Birthe Lemvig, Katrine Laura Rasmussen, Julie Smith, Thore Hillig
Immature granulocyte percentage (IG%) is an important biomarker for infection control. We observed spurious cases where the IG% was dramatically underestimated on the automated Sysmex XN-series hehmatology analyzer compared with manual differential. These cases were associated with high values of “Neutrophil Reactivity Intensity” (NEUT-RI), which should reflect the metabolic activity of the neutrophils
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Erythrophagocytosis by dysplastic neutrophils and their precursors Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-12 Yue Zhao, Sophie Parks, Imran Siddiqi, Endi Wang
A 67-year-old male patient presented with chronic fatigue and shortness of breath. Physical examination was unremarkable. Laboratory evaluation demonstrated mild pancytopenia: white blood cells, 3.85 × 109/L (neutrophils, 1.89 × 109/L, and monocytes, 0.75 × 109/L); hemoglobin, 113 g/L; and platelets, 104 × 109/L. Additional tests revealed no laboratory evidence of hemolysis. Bone marrow examination
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Platelet indices and angiogenesis markers in hypertensive disorders of pregnancy Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-12 Laura Mattana Dionisio, Giovani Marino Favero
Activated platelets exert a key role in the pathogenesis of preeclampsia (PE). There is evidence of distinctive patterns of platelet indices in PE in comparison to healthy pregnancies, therefore these indices can be potential tools for PE detection, risk stratification, and management. Considering the vascular aspects of its pathophysiology, PE is characterized by the increased levels of soluble FMS-like
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Presence of leukemic clone-specific immunoglobulin heavy chain rearrangements in neonatal blood spots of children with B-cell precursor acute lymphoblastic leukemia Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-06 Natasa Kacanski, Jovanka Kolarovic, Tatjana Kostic, Irena Marjanovic, Dragana Janic, Sonja Pavlovic, Teodora Karan-Djurasevic
Childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL) can be traced back to birth using leukemic clone-specific immunoglobulin heavy chain (IGH) rearrangements, implying prenatal origin of this disease.
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Bone marrow adipocyte: Origin, biology and relationship with hematological malignancy Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-05 Yan Wang, Chunxia Yang, Junzhao Wan, Ping Liu, Hantao Yu, Xiaoyan Yang, Dan Ma
Bone marrow adipose tissue (BMAT) has been histologically recognized for decades. In this study, we performed a bibliometric analysis to quantitatively analyze the clusters of keywords of BMAT and hematopoiesis to better understand BMAT and hematopoiesis. Starting with conclusive keywords, our results demonstrated that BMAds is distinct from extramedullary adipose tissues and maintains a routine but
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Sysmex-derived COVID-19 prognostic score as an early prognostic marker for severity of the COVID-19 disease Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-03 Martin Blažek, Filip Vrbacký, Ilona Fátorová, Klára Mirská, Pavel Žák
Coronavirus disease 2019 (COVID-19) is a life-threatening disease with a heterogeneous course. Even some young patients are at increased risk of severe course or death, as they can face severe complications. It would be very useful to have a cheap and easily available marker to predict COVID-19 course in the early stages of the disease. The COVID-19 prognostic score could be a very useful clinical
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Effect of prothrombin Belgrade mutation, causing antithrombin resistance, on fibrin clot properties Int. J. Lab. Hematol. (IF 3.0) Pub Date : 2023-11-02 Sofija Dunjic Manevski, Marija Cumbo, Iva Pruner, Maja Gvozdenov, Branko Tomic, Apostolos Taxiarchis, Jovan Antovic, Valentina Djordjevic
Prothrombin Belgrade mutation is the result of the c.1787G>A substitution in the prothrombin gene. It is located in the antithrombin and sodium binding site and leads to impaired inactivation of thrombin by antithrombin, resulting in antithrombin resistance and thrombotic disorders. However, it negatively affects sodium binding and may have hypocoagulant effects. Considering that prothrombin Belgrade