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Scoping review of factors associated with stem cell mobilisation and collection in allogeneic stem cell donors medRxiv. Hematol. Pub Date : 2024-03-16 Rachel Peck, Amber Knapp-Wilson, Kate Burley, Carolyn Doree, James Griffin, Andrew Mumford, Simon Stanworth, Kirsty Sharplin
Background: There is a large inter-individual variation in CD34+ cell yield after G-CSF mobilisation and collection from peripheral blood in healthy allogenic haematopoietic stem cell donors. Donor characteristics including gender and age, baseline and precollection blood results, mobilisation factors and collection factors have been associated with CD34+ cell concentration in the blood after G-CSF
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Everolimus and mycophenolate mofetil effectively prevent GvHD in children with severe acute kidney injury undergoing allogeneic HSCT medRxiv. Hematol. Pub Date : 2024-03-16 Felix Zirngibl, Pimrapat Gebert, Bianca Materne, Michael Launspach, Annette Kuenkele, Patrick Hundsoerfer, Sandra Cyrull, Hedwig E Deubzer, Joern Sven Kuehl, Angelika Eggert, Peter Lang, Lena Oevermann, Arend von Stackelberg, Johannes H Schulte
Allogeneic hematopoietic stem cell transplantation (HSCT) serves as a therapeutic intervention for various pediatric diseases. Acute kidney injury afflicts 21-84% of pediatric HSCT cases, significantly compromising clinical outcomes. This retrospective single-institution analysis scrutinized the practice of substituting nephrotoxic ciclosporin A with the everolimus/mycophenolate mofetil combination
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Sickle Cell Disease and Uterine Fibroids: Evaluation of the Prevalence of Fibroids across Sickle Cell Genotypes medRxiv. Hematol. Pub Date : 2024-03-08 Jonathan G. Martin, Alexis M. Medema, Blaire K. Rikard, Gabrielle van den Hoek, Miriam Chisholm
Introduction: Uterine fibroids are known to affect >80% of premenopausal American women of African descent, and sickle cell disease is known to disproportionately affect people of varying geographical ancestries, particularly those of sub-Saharan African descent. However, previous studies have suggested the two pathologies less frequently co-occur. This study aims to evaluate the prevalence of uterine
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Calling for diversity: improving transfusion safety through high-throughput blood group microarray genotyping medRxiv. Hematol. Pub Date : 2024-03-07 Michael Wittig, Tim Alexander Steiert, Hesham ElAbd, Frauke Degenhardt, Luca Valenti, Daniele Prati, Luisa Ronzoni, Luis Bujanda, Jesus M. Banales, Natalia Blay, Pietro Invernizzi, Maria Buti, Agustin Albillos, Javier Fernandez, Nicoletta Sacchi, Antonio Julia, Anna Latiano, Rafael de Cid, Mauro D'Amato, Rosanna Asselta, Matthias Laudes, Wolfgang Lieb, David Juhl, Christoph Gassner, Andre Franke
Blood transfusions, conducted between donors compatible in their red blood cell (RBC) antigens, play a life-saving role in transfusion medicine. Genetic differences at blood group loci between ethnicities result in diversity and altered frequency of RBC antigens that need to be considered in blood transfusion. Consequently, comprehensive, and accurate blood group antigen typing is especially relevant
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Balancing donor health and plasma collection: a systematic review of the impact of plasmapheresis frequency medRxiv. Hematol. Pub Date : 2024-03-06 Tine D'aes, Katja van den Hurk, Natalie Schroyens, Susan Mikkelsen, Pieter Severijns, Emmy De Buck, Peter O'Leary, Pierre Tiberghien, Veerle Compernolle, Christian Erikstrup, Hans Van Remoortel
Most plasma used for manufacturing plasma-derived medicinal products (PDMPs) such as albumin, immunoglobulin (Ig), and clotting factors is obtained from source plasma collected via plasmapheresis, the majority of which is contributed by the United States (US). While the demand for PDMPs continues to rise, it remains unclear whether high-frequency plasmapheresis, such as the twice-weekly plasma donation
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How many labels do I need? Self-supervised learning strategies for multiple blood parasites classification in microscopy images medRxiv. Hematol. Pub Date : 2024-03-01 Roberto Mancebo-Martin, Lin Lin, Elena Dacal, Miguel Luengo-Oroz, David Bermejo-Pelaez
Bloodborne parasitic diseases such as malaria, filariasis or chagas pose significant challenges in clinical diagnosis, with microscopy as the primary tool for diagnosis. However, limitations such as time-consuming processes and the dependence on trained microscopists is critical, particularly in resource-constrained settings. Deep learning techniques have shown value to interpret microscopy images
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Microliter whole blood neutrophil assay preserving physiological lifespan and functional heterogeneity medRxiv. Hematol. Pub Date : 2024-02-27 Chao Li, Nathan W Hendrikse, Makenna Mai, Mehtab A Farooqui, Zach Argall-Knapp, Jun Sung Kim, Emily A Wheat, Terry Juang
For in vitro neutrophil functional assays, neutrophils are typically isolated from whole blood, having the target cells exposed to an artificial microenvironment with altered kinetics. Isolated neutrophils exhibit limited lifespans of only a few hours ex vivo, significantly shorter than the 3-5 day lifespan of neutrophils in vivo. In addition, due to neutrophil inherently high sensitivity, neutrophils
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TP53 mutation screening for patients at risk of myeloid malignancy medRxiv. Hematol. Pub Date : 2024-02-08 Devdeep Mukherjee, Rialnat A. Lawal, Courtney D. Fitzhugh, Christopher S. Hourigan, Laura W. Dillon
There is increasing recognition of the risk of developing therapy-related myeloid malignancy, including after cellular therapy. While retrospective studies have implicated pre-existing TP53 mutated hematopoietic clones as a common causative mechanism, no prospective screening to identify those patients at greatest risk is currently possible. We demonstrate that ultradeep DNA-sequencing prior to therapy
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Optimizing Stem Cell Infusion Timing in the Prevention of Acute Graft versus Host Disease medRxiv. Hematol. Pub Date : 2024-02-07 Yiwen Hou, Yue Wu, Zhonglin Zhang, Liang Wang, Zhiwei Liu, Baolin Tang, Kaidi Song, Guangyu Sun, Xiaoyu Zhu, Cheng Zhan
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a cornerstone treatment for a broad spectrum of malignant and nonmalignant hematological disorders. However, the success of allo-HSCT is often overshadowed by acute graft-versus-host disease (aGVHD), a life-threatening complication with limited preventive options. Here, we found that the incidence and severity of aGVHD after allo-HSCT
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Analysis of 3,760 hematologic malignancies reveals rare transcriptomic aberrations of driver genes medRxiv. Hematol. Pub Date : 2024-02-05 Xueqi Cao, Sandra Huber, Ata Jadid Ahari, Franziska R. Traube, Marc Seifert, Christopher C. Oakes, Polina Secheyko, Sergey Vilov, Ines Scheller, Nils Wagner, Vicente A. Yepez, Piers Blombery, Torsten Haferlach, Matthias Heinig, Leonhard Wachutka, Stephan Hutter, Julien Gagneur
Background Rare oncogenic driver events, particularly affecting the expression or splicing of driver genes, are suspected to substantially contribute to the large heterogeneity of hematologic malignancies. However, their identification remains challenging. Methods To address this issue, we generated the largest dataset to date of matched whole genome sequencing and total RNA sequencing of hematologic
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Increased Frequency of Clonal Hematopoiesis of Indeterminate Potential in Bloom Syndrome Probands and Carriers medRxiv. Hematol. Pub Date : 2024-02-06 Isabella Lin, Angela Wei, Tsumugi A Gebo, PC Boutros, Maeve Flanagan, Nicole Kucine, C Cunniff, VA Arboleda, VY Chang
Background Bloom Syndrome (BSyn) is an autosomal recessive disorder caused by biallelic germline variants in BLM, which functions to maintain genomic stability. BSyn patients have poor growth, immune defects, insulin resistance, and a significantly increased risk of malignancies, most commonly hematologic. The malignancy risk in carriers of pathogenic variants in BLM (BLM variant carriers) remains
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Facilitating clinical trials in Polycythemia vera (PV) by identifying patient cohorts at high near-term risk of thrombosis using rich data and machine learning. medRxiv. Hematol. Pub Date : 2024-01-22 Ghaith Abu-Zeinah, spencer krichevsky, Katie Erdos, Joseph Scandura
Thrombosis remains the leading cause of morbidity and mortality for patients (pts) with polycythemia vera (PV), yet PV clinical trials are not powered to identify interventions that improve thrombosis-free survival (TFS). Such trials are infeasible in a contemporary PV cohort, even when selecting high-risk pts based on Age >60 and thrombosis history, because thousands of patients would be required
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Proliferative History Is a Novel Driver of Clinical Outcome in Splenic Marginal Zone Lymphoma medRxiv. Hematol. Pub Date : 2024-01-17 Helen Parker, Amatta Mirandari, Carolina Jaramillo Oquendo, Martí Duran-Ferrer, Benjamin Stevens, Lara Buermann, Harindra E. Amarasinghe, Jaya Thomas, Latha Kadalayil, Louise Carr, Shama Syeda, Methusha Sakthipakan, Marina Parry, Zadie Davis, Neil McIver-Brown, Aliki Xochelli, Sarah Ennis, Lydia Scarfo, Paolo Ghia, Christina Kalpadakis, Gerassimos Pangalis, Davide Rossi, Simon Wagner, Matthew Ahearne
The epiCMIT (epigenetically-determined Cumulative MIToses) mitotic clock traces B-cell mitotic history via DNA methylation changes in heterochromatin and H3K27me3-containing chromatin. While high scores correlated with poor outcomes in CLL and MCL, its prognostic significance in SMZL remains unknown. Derived from 142 SMZL cases using DNA methylation microarrays, epiCMIT values were correlated with
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Comparing DOACs with warfarin in AF patients with chronic kidney disease or valvular disease: A systematic review and meta-analysis medRxiv. Hematol. Pub Date : 2024-01-15 Aileen Liang, Cathy Wang, Alla E Iansavichene, Alejandro Lazo-Langner
Objective To analyze the safety and efficacy of different direct oral anticoagulant agents (DOACs) compared to warfarin in patients with concomitant atrial fibrillation (AF) and valvular disease or concomitant AF and chronic kidney disease (CKD).
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An Ensemble Model for Acute Myeloid Leukemia Risk Stratification Recommendations by Combining Machine Learning with Clinical Guidelines medRxiv. Hematol. Pub Date : 2024-01-09 Ming-Siang Chang, Cheng-Hong Tsai, Wen-Chien Chou, Hwei-Fang Tien, Hsin-An Hou, Chien-Yu Chen
Acute Myeloid Leukemia (AML) is a complex disease requiring accurate risk stratification for effective treatment planning. This study introduces an innovative ensemble machine learning model integrated with the European LeukemiaNet (ELN) 2022 recommendations to enhance AML risk stratification. The model demonstrated superior performance by utilizing a comprehensive dataset of 1,213 patients from National
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Single-cell analysis of bone marrow CD8+ T cells in Myeloid Neoplasms predicts response to treatment with Azacitidine medRxiv. Hematol. Pub Date : 2023-12-31 Athanasios Tasis, Nikos E. Papaioannou, Maria Grigoriou, Nikolaos Paschalidis, Katerina Loukogiannaki, Anastasia Filia, Kyriaki Katsiki, Eleftheria Lamprianidou, Vasileios Papadopoulos, Christina Maria Rimpa, Antonios Chatzigeorgiou, Ioannis P. Kourtzelis, Petroula Gerasimou, Ioannis Kyprianou, Paul Costeas, Panagiotis Liakopoulos, Konstantinos Liapis, Petros Kolovos, Triantafyllos Chavakis, Themis
Treatment with hypomethylating agents (HMA), and specifically azacitidine (AZA), is the standard of care for patients with higher-risk myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) that are not eligible to receive intensive chemotherapy. Despite research efforts, it is not possible to predict response to treatment with HMA. In this study, we aimed to identify immune cell signatures
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von Willebrand factor-related values can predict bleeding events in patients with left ventricular assist devices medRxiv. Hematol. Pub Date : 2023-12-17 Ryuichi Taketomi, Ko Sakatsume, Shintaro Katahira, Kota Goto, Misako Suzuki, Zuo Yunan, Konosuke Sasaki, Midori Miyatake, Katsuhiro Hosoyama, Koki Ito, Yusuke Suzuki, Goro Takahashi, Kiichiro Kumagai, Hisanori Horiuchi, Yoshikatsu Saiki
Background Recent advances in left ventricular assist device (LVAD) therapy have significantly contributed to the improved management of severe heart failure. The unignorable risk of bleeding events associated with the device therapy, however, remains to be addressed. Predictive factors for bleeding events are not fully defined.
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The impact of COVID-19 vaccines on patients with immune thrombocytopenic purpura: A protocol for a systematic review and meta-analysis medRxiv. Hematol. Pub Date : 2023-12-13 Yangyang Li, Demin Kong, Yicheng Ding, Jinhuan Wang
Introduction Immune thrombocytopenic purpura (ITP) is characterized by a decrease in platelet counts and can be triggered by various factors, such as viral infections and vaccinations. Concerns have emerged regarding potential links between the vaccines for COVID-19 and the worsening of ITP. This systematic review aims to comprehensively assess the impact of COVID-19 vaccines on patients with ITP,
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Genomic determinants of response and resistance to inotuzumab ozogamicin in B-cell ALL medRxiv. Hematol. Pub Date : 2023-12-09 Yaqi Zhao, Nicholas J Short, Hagop M Kantarjian, Ti-Cheng Chang, Pankaj S Ghate, Chunxu Qu, Walid Macaron, Nitin Jain, Beenu Thakral, Aaron H Phillips, Joseph Khoury, Guillermo Garcia-Manero, Wenchao Zhang, Yiping Fan, Hui Yang, Rebecca S Garris, Lewis F Nasr, Richard W Kriwacki, Kathryn G Roberts, Marina Konopleva, Elias J Jabbour, Charles G Mullighan
Inotuzumab ozogamicin (InO) is an antibody-drug conjugate that delivers calicheamicin to CD22-expressing cells. In a retrospective cohort of InO treated patients with B-cell acute lymphoblastic leukemia, we sought to understand the genomic determinants of response to InO. Acquired CD22 mutations were observed in 11% (3/27) of post-InO relapsed tumor samples. There were multiple CD22 mutations per sample
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Concordance of Truvian's Benchtop Blood Testing Platform to Central Laboratory Testing medRxiv. Hematol. Pub Date : 2023-11-29 Renee Higgins, Nicholas Haase, Patrick Desmond, Ian Levine, Clara Romero, Brian Fernández, Eumene Lee, Mike Adams, Derek Arndt,, Greg Grabarek, Ju Young Kim, Rachel Krupa, Ginger Mina, Ryan Morgan, John Poland, Galen Reed, Robin Richardson, Kelline Rodems, Astrid Schroeder, Maike Zimmermann, Florence Lee, Dena Marrinucci
Introduction - Routine blood tests play an essential role in modern healthcare, but their administration, processing, and reporting under the current centralized testing model is slow, inefficient, and cumbersome for patients and providers especially in outpatient settings. Truvian's benchtop blood testing platform, in late-stage development, aims to decentralize and streamline routine blood testing
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Multiparametric MRI detects multi-organ impairment in patients with chronic myeloid neoplasms with normal serum biomarkers medRxiv. Hematol. Pub Date : 2023-11-23 Sophie Reed, Charlie Diamond, Samiya Mahmood, Soubera Rymell, Michael Smith, Michele Pansini, Bethan Psaila, Adam J. Mead, Helena Thomaides-Brears, Onima Chowdhury
Assessment of organ impairment in patients with chronic myeloid neoplasms is pivotal in selecting treatments and for accurate prognostication of patient outcomes. In order to determine the multi-organ health of patients with chronic myeloid neoplasms, we conducted a prospective, observational study utilising a novel MRI technology which quantitatively assesses the health of multiple organs in one scan
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UBTF Tandem Duplications in Pediatric MDS and AML: Implications for Clinical Screening and Diagnosis medRxiv. Hematol. Pub Date : 2023-11-13 Juan M. Barajas, Masayuki Umeda, Lisett Contreras, Mahsa Khanlari, Tamara Westover, Michael P. Walsh, Emily Xiong, Chenchen Yang, Brittney Otero, Marc Arribas-Layton, Sherif Abdelhamed, Guangchun Song, Xiaotu Ma, Melvin E. Thomas, Jing Ma, Jeffery M. Klco
Recent genomic studies in adult and pediatric acute myeloid leukemia (AML) demonstrated recurrent in-frame tandem duplications (TD) in exon 13 of upstream binding transcription factor (UBTF). These alterations, which account for ~4.3% of AMLs in childhood and up to 3% in adult AMLs under 60, are subtype-defining and associated with poor outcomes. Here, we provide a comprehensive investigation into
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Analysis and Evaluation of Blood Screening Testing Strategies in Nagqu, Tibet: One of The Highest Cities in the World medRxiv. Hematol. Pub Date : 2023-11-09 Liang Zang, Lei Zhou, Yaxin Fan, Xiaohua Liang, Ji Duo, Hao Lin, Rui Bai, Mei Yang, Chao Dan
The aim of this study was to evaluate the performance of blood screening strategies in Nagqu City, with a focus on serological and nucleic acid testing effectiveness. Analysis of blood donation data from 2017 to 2023 revealed a gradual increase in local blood donation rates, but the rate of unqualified donations remained higher than the national average. Serological performance verification showed
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Biologic and clinical features of childhood gamma delta T-ALL: identification of STAG2/LMO2 γδ T-ALL as an extremely high risk leukemia in the very young medRxiv. Hematol. Pub Date : 2023-11-08 Shunsuke Kimura, Petri Polonen, Lindsey Montefiori, Chun Shik Park, Ilaria Iacobucci, Allen EJ Yeoh, Andishe Attarbaschi, Andrew S. Moore, Anthony Brown, Atsushi Manabe, Barbara Buldini, Burgess B. Freeman, Chelsey Chen, Cheng Cheng, Chiew Kean Hui, Chi-Kong Li, Ching-Hon Pui, Chunxu Qu, Daisuke Tomizawa, David T. Teachey, Elena Varotto, Elisabeth M Paietta, Elizabeth D. Arnold, Franco Locatelli, Gabriele
PURPOSE: Gamma delta T cell receptor-positive acute lymphoblastic leukemia (γδ T-ALL) is a high-risk but poorly characterized disease. METHODS: We studied clinical features of 200 pediatric γδ T-ALL, and compared the prognosis of 93 cases to 1,067 protocol matched non-γδ T-ALL. Genomic features were defined by transcriptome and genome sequencing. Experimental modeling was used to examine the mechanistic
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Mimicking Clinical Trials with Synthetic Acute Myeloid Leukemia Patients Using Generative Artificial Intelligence medRxiv. Hematol. Pub Date : 2023-11-08 Jan-Niklas Eckardt, Waldemar Hahn, Christoph Roellig, Sebastian Stasik, Uwe Platzbecker, Carsten Mueller-Tidow, Hubert Serve, Claudia D. Baldus, Christoph Schliemann, Kerstin Schaefer-Eckart, Maher Hanoun, Martin Kaufmann, Andreas Burchert, Christian Thiede, Johannes Schetelig, Martin Sedlmayr, Martin Bornhaeuser, Markus Wolfien, Jan Moritz Middeke
Clinical research relies on high-quality patient data, however, obtaining big data sets is costly and access to existing data is often hindered by privacy and regulatory concerns. Synthetic data generation holds the promise of effectively bypassing these boundaries allowing for simplified data accessibility and the prospect of synthetic control cohorts. We employed two different methodologies of generative
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Altered Platelet-Megakaryocyte Endocytosis and Trafficking of Albumin and Fibrinogen in RUNX1 Haplodeficiency medRxiv. Hematol. Pub Date : 2023-10-23 Fabiola Del Carpio-Cano, Guangfen Mao, Lawrence E Goldfinger, Jeremy Wurtzel, Afaque Mohammad Alam, Liying Guan, Kiwon Lee, Mortimer Poncz, Angara Koneti Rao
Platelet α-granules have numerous proteins, some synthesized by megakaryocytes (MK) and others not synthesized but incorporated by endocytosis, an incompletely understood process in platelets/MK. Germline RUNX1 haplodeficiency, referred to as familial platelet defect with predisposition to myeloid malignancies (FPDMM), is associated with thrombocytopenia, platelet dysfunction and granule deficiencies
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Biomarkers of bleeding and venous thromboembolism in patients with acute leukemia medRxiv. Hematol. Pub Date : 2023-10-19 Yohei Hisada, Sierra J Archibald, Karan Bansal, Yanjun Chen, Chen Dai, Sindhu Dwarampudi, Nora Balas, Lindsey Hageman, Nigel S Key, Smita Bhatia, Ravi Bhatia, Nigel Mackman, Radhika Gangaraju
Background: Coagulopathy and associated bleeding and venous thromboembolism (VTE) are major causes of morbidity and mortality in patients with acute leukemia. The underlying mechanisms of these complications have not been fully elucidated. Objectives: To evaluate the associations between biomarker levels and bleeding and VTE in acute leukemia patients. Patients/Method: We examined plasma levels of
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Differential clinical characteristics across traditional Chinese medicine (TCM) syndromes in patients with sickle cell disease medRxiv. Hematol. Pub Date : 2023-10-16 Ying Wang, David D Wang, Andrew Q Pucka, Andrew RW O'Brien, Steven E Harte, Richard E Harris
Background: Pain is a common, debilitating, and poorly understood complication of sickle cell disease (SCD). The need for clinical pain management of SCD is largely unmet and relies on opioids as the main therapeutic option, which leads to a decreased quality of life (QoL). According to the literature, acupuncture has shown certain therapeutic effects for pain management in SCD. However, these clinical
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Longitudinal viscosity of blood plasma for rapid COVID-19 prognostics medRxiv. Hematol. Pub Date : 2023-10-15 Jennifer Illibauer, Tamara Clodi-Seitz, Alexander Zoufaly, Judith H Aberle, Wolfgang J Weninger, Manuela Foedinger, Kareem Elsayad
Blood Plasma Viscosity (PV) is an established biomarker for numerous diseases. While PV colloquially refers to the shear viscosity, there is a second viscosity component--the bulk viscosity--that describes the irreversible fluid compressibility on short time scales. The bulk viscosity is acutely sensitive to solid-like suspensions, and obtainable via the longitudinal viscosity from acoustic attenuation
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Brain network hypersensitivity underlies pain crises in sickle cell disease medRxiv. Hematol. Pub Date : 2023-10-09 Pangyu Joo, Minkyung Kim, Brianna Kish, Vidhya Vijayakrishnan Nair, Yunjie Tong, Steven E Harte, Richard E Harris, UnCheol Lee, Ying Wang
Sickle cell disease (SCD) is a genetic disorder causing blood vessel blockages and painful Vaso-occlusive crises (VOCs). VOCs, characterized by severe pain due to blocked blood flow, are recurrent and unpredictable, posing challenges for preventive strategies. In this study we propose explosive synchronization (ES), a phenomenon characterized by abrupt brain network phase transitions, as a novel approach
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Characteristics, treatment and outcomes of myelodysplastic syndrome in two Finnish hospital districts medRxiv. Hematol. Pub Date : 2023-10-06 Samuli Tuominen, Tatu Miettinen, Christina Dunweber
Abstract Introduction: Myelodysplastic syndromes (MDS) are hematologic malignancies characterized by changes in haematopoiesis and a high risk for progressing into acute myeloid leukemia (AML). In this retrospective registry based real-world study, from two Finnish hospital data lakes we characterized specialised health care treated MDS patients, their treatment landscape, outcomes, and healthcare
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Tumor Purity-Related Genes for Predicting the Prognosis and Drug Sensitivity of DLBCL Patients medRxiv. Hematol. Pub Date : 2023-10-05 Zhenbang Ye, Ning Huang, Yongliang Fu, Rongle Tian, Wenting Huang
Background: Diffuse large B-cell lymphoma (DLBCL) is the predominant type of malignant B-cell lymphoma. Although various treatments have been developed, the limited efficacy calls for more and further exploration of its characteristics. Methods: Datasets from Gene Expression Omnibus (GEO) database were used for identifying the tumor purity of DLBCL. Survival analysis was employed for analyzing the
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Demographic profile, risk stratification, clinical characteristics, and treatment outcome of patients with multiple myeloma: A five-year retrospective study at a tertiary hospital in Ethiopia medRxiv. Hematol. Pub Date : 2023-10-03 Ephrem Haile, Adugna Tassew Tebabel, Amha Gebremedhin, Abdulaziz Abubeker Sherif, Fissehatsion Tadesse
Background Patients with multiple myeloma are being seen at an increasing frequency in different indigenous African population. However, local data regarding the demographic profile, clinical characteristics, risk stratification, and treatment outcome of these patients is lacking. This study was designed to fill this existing gap in our setup. Hence, it will aid in the revision of treatment guidelines
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Epidemiology and Nomogram Development for Chronic Eosinophilic Leukemia, Not Otherwise Specified (CEL-NOS): Insights from the SEER Database medRxiv. Hematol. Pub Date : 2023-10-02 Fan Wang
Background: Chronic Eosinophilic Leukemia, Not Otherwise Specified (CEL-NOS), a rare and intricate hematological disorder characterized by uncontrolled eosinophilic proliferation, presents clinical challenges owing to its infrequency. This study aimed to investigate the epidemiology and develop a prognostic nomogram for CEL-NOS patients. Methods: Utilizing the Surveillance, Epidemiology and End Results
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Hematologic setpoints are a stable and patient-specific deep phenotype medRxiv. Hematol. Pub Date : 2023-09-28 Brody H Foy, Rachel Petherbridge, Maxwell Roth, Christopher Mow, Hasmukh R Patel, Chhaya H Patel, Samantha N Ho, Evie Lam, Konrad J Karczewski, Veronica Tozzo, John H Higgins
The complete blood count is an important screening tool for healthy adults and is the most commonly ordered test at periodic physical exams. However, results are usually interpreted relative to one-size-fits-all reference intervals, undermining the goal of precision medicine to tailor medical care to the needs of individual patients based on their unique characteristics. Here we show that standard
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Assessment of Knowledge and Attitude towards Blood Donation among Blood Donors in Jordan medRxiv. Hematol. Pub Date : 2023-09-25 Rahaf Omaish, Zahraa Al-Fayyadh, Shifar Al-Habashneh, Safa Al-Mashhdi, Salah Khasawneh,, Isam Naber, Sana Bourghli, Tariq Al-Adily, Azmi Mahafzah, Firas Al-Fararjeh, Maher a Sughayer
Objectives: This study will identify blood donor characteristics in Jordan. The study aims to investigate the extent of awareness and knowledge regarding blood donation among blood donors and to explore their attitudes towards blood donation in general. It also highlights various motivational factors and obstacles, which influence the decision to donate blood among Jordanian donors. Background: Recruitment
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Screening of Thalassemia Carriers, Hemoglobin Variants, and Comparison of Hematological Parameters between Children of Bangladesh and Forcibly Displaced Myanmar Nationals medRxiv. Hematol. Pub Date : 2023-09-21 Rumana Mahtarin, Kasrina Azad, Mohabbat Hossain, Mst. Sharmin Aktar Mukta, Mohammad Tanbir Habib, Abu Bakar Siddik, Nishat Sultana, Zannat Kawser, Umme Kulsum, Nusrat Sultana, Farjana Akther Noor, Ahmad Zubair Mahdi, Muhammad Asaduzzaman, Md. Ahashan Habib, Emran Kabir Chowdhury, Firdausi Qadri, Mst. Noorjahan Begum, A.H.M Nurun Nabi
Thalassemia is a hereditary blood disorder with different severity spectrums. This study aimed to assess thalassemia screening rates between children of Bangladesh and selected camps of Forcibly Displaced Myanmar Nationals (FDMN) in Cox’s Bazar in Bangladesh and compare the hematological parameters among the screening groups. Complete blood count (CBC) analysis and hemoglobin electrophoresis for each
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Pharmacometric assessment of primaquine induced haemolysis in glucose-6-phosphate dehydrogenase deficiency medRxiv. Hematol. Pub Date : 2023-09-20 Sasithon Pukrittayakamee, Podjanee Jittamala, James Andrew Watson, Borimas Hanboonkunupakarn, Pawanrat Leungsinsiri, Kittiyod Poovorawan, Kesinee Chotivanich, Germana Bancone, Cindy S Chu, Mallika Imwong, Nicholas PJ Day, Walter RJ Taylor, Nicholas J White
Background Primaquine is an 8-aminoquinoline antimalarial. It is the only widely available treatment to prevent relapses of Plasmodium vivax malaria. The 8-aminoquinolines cause dose dependent haemolysis in glucose-6-phosphate dehydrogenase deficient (G6PDd) individuals. G6PDd is common in malaria endemic areas but testing is often not available. As a consequence primaquine is underused. Methods We
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Platelet dysfunction reversal with cold-stored vs. room temperature-stored platelet transfusions. medRxiv. Hematol. Pub Date : 2023-09-17 Valery J Kogler, Jeffrey Miles, Tahsin Ozpolat, S Lawrence Bailey, Daire A Byrne, Morgan Bawcom-Randall, Yi Wang, Hannah J Larsen, Franklin Reed, Xiaoyun Fu, Moritz Stolla
Background: Platelets are stored at room temperature for 5-7 days (RSP). Due to frequent and severe shortages, the FDA recently approved up to 14-day cold-stored platelets in plasma (CSP). However, the post-transfusion function of CSP is unknown and it is unclear which donors are best suited to provide either RSP and/or CSP. Objective: To evaluate the post-transfusion function and predictors of post-transfusion
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Cellular aging is accelerated in the malignant clone of myeloproliferative neoplasms medRxiv. Hematol. Pub Date : 2023-09-12 Margherita Vieri, Vithurithra Tharmapalan, Milena Kalmer, Julian Baumeister, Milos Nikolic, Matthis Schnitker, Martin Kirschner, Niclas Flosdorf, Marcelo A Szymanski de Toledo, Martin Zenke, Steffen Koschmieder, Tim Hendrik Brummendorf, Fabian Beier, Wolfgang Wagner
Myeloproliferative neoplasms (MPNs) are caused by somatic driver mutations, such as JAK2V617F, which might also affect cellular aging and senescence. Here, we analyzed the heterogeneity of aging in MPN patients and if this can be used to specifically target malignant cells. The mean epigenetic age was significantly accelerated in 129 MPN patients across all disease-entities, whereas premature telomere
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Dietary risk factors for monoclonal gammopathy of undetermined significance in a racially diverse population medRxiv. Hematol. Pub Date : 2023-09-07 Janine M Joseph, Jens Hillengass, Li Tang, Alexander M Lesokhin, Saad Usmani, Ola Landgren, Kirsten B Moyisch, Susan E McCann, Urvi A Shah
Importance: Monoclonal gammopathy of undetermined significance (MGUS), a precursor condition of multiple myeloma, is associated with shorter lifespan and cardiac, renal, neurologic, and immune-related comorbidities. However, there is little known about modifiable risk factors, such as diet, for this condition. Objective: To determine if risk of MGUS is associated with dietary factors in a racially
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Blood transfusion in care of patients with Visceral Leishmaniasis: a review of practices in therapeutic efficacy studies medRxiv. Hematol. Pub Date : 2023-09-06 Prabin Dahal, Sauman Singh, James Wilson, Glaucia Cota, Koert Ritmeijer, Ahmed Musa, Fabiana Alves, Kasia Stepniewska, Philippe J Guerin
Anaemia is a common presentation feature in patients with visceral leishmaniasis (VL). Blood transfusion remains an important aspect of patient management in VL. However, triggers considered for making decisions on transfusion are poorly understood. This review is based on the Infectious Diseases Data Observatory (IDDO) VL clinical trials library, a database of all published efficacy studies since
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Persistent IDH mutations are not associated with increased relapse or death in patients with IDH-mutated acute myeloid leukemia undergoing allogeneic hematopoietic cell transplant with post-transplant cyclophosphamide medRxiv. Hematol. Pub Date : 2023-08-21 Niveditha Ravindra, Laura W Dillon, Gege Gui, Matthew Smith, Lukasz P Gondek, Richard J Jones, Adam Corner, Christopher S Hourigan, Alexander J Ambinder
The presence of measurable residual disease (MRD) prior to an allogeneic hematopoietic transplant (alloHCT) in Acute Myeloid Leukemia (AML) has been shown to be associated with an increased risk of post-transplant relapse. Since the Isocitrate Dehydrogenase genes (IDH1/2) are mutated in a considerable proportion of patients with AML, we studied if these mutations would serve as useful targets for MRD
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Social determinants of adult health outcomes in Sickle cell disease: a scoping review protocol medRxiv. Hematol. Pub Date : 2023-08-16 Alphanso Lorenzo Blake, Nadia Bennett, Trevor S Ferguson, Seeromanie Harding, Monika Asnani
Objective: To summarize the evidence and identify gaps in the literature related to the impact of social factors on health outcomes across the life course for persons with Sickle Cell Disease. Introduction: Sickle cell disease (SCD) is a group of genetic diseases where abnormal hemoglobin is associated with recurrent complications across the life course which results in increased morbidity or death
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Elevated pre-treatment Neutrophil-to-lymphocyte and neutrophil-to-lymphocyte-platelet ratios are associated with a high risk of in-hospital death among patients with prostate cancer in Nigeria medRxiv. Hematol. Pub Date : 2023-08-15 Jude Ogechukwu Okoye, Vivian Ifunanya Ogbonnaya, Michael Emeka Chiemeka, Samuel Ifedioranma Ogenyi
Introduction: In the last decade, there is an increasing mortality rate among patients diagnosed with prostate cancer (PCa) in West Africa. To identify the causes of the high mortality rate, this study analyzed the occurrence of high-grade tumours and the presence of BRCA2 mutation. It also assessed the systemic inflammatory indices as prognostic tools in low-resource settings. Methods: This study
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Genome-wide DNA methylation-analysis delineates blastic plasmacytoid dendritic cell neoplasm from related entities and identifies distinct molecular features medRxiv. Hematol. Pub Date : 2023-08-04 Axel Kuenstner, Julian Schwarting, Hanno Witte, Pengwei Xing, Veronica Bernard, Stephanie Stoelting, Philipp Lohneis, Florian Janke, Maede Salehi, Xingqi Chen, Kathrin Kusch, Holger Sueltmann, Emil Chteinberg, Anja Fischer, Reiner Siebert, Nikolas von Bubnoff, Hartmut Merz, Hauke Busch, Alfred Feller, Niklas Gebauer
Blastic plasmacytoid dendritic cell neoplasm (BPDCN) constitutes a rare and aggressive malignancy originating from plasmacytoid/common dendritic cells (pDCs/cDCs) with a primarily cutaneous tropism followed by dissemination to the bone marrow and other organs. We conducted a genome-wide analysis of the tumor methylome in an extended cohort of 45 BPDCN patients supplemented by WES (n=54) and RNA-seq
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A signature of platelet reactivity in CBC scattergrams reveals genetic predictors of thrombotic disease risk medRxiv. Hematol. Pub Date : 2023-07-27 Hippolyte Verdier, Patrick Thomas, Joana Batista, Carly Kempster, Harriet McKinney, Nicholas Gleadall, John Danesh, Andrew Mumford, Johan Heemskerk, Willem H Ouwehand, Kate Downes, William J Astle, Ernest Turro
Genetic studies of platelet reactivity (PR) phenotypes may identify novel antiplatelet drug targets. However, discoveries have been limited by small sample sizes (n<5,000) due to the complexity of measuring PR. We trained a model to predict PR from complete blood count (CBC) scattergrams. A GWAS of this phenotype in 29,806 blood donors identified 21 distinct associations implicating 20 genes, of which
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Miniaturized microscope for non-invasive imaging of leukocyte-endothelial interactions in human microcirculation medRxiv. Hematol. Pub Date : 2023-07-26 Arutyun Bagramyan, Charles P. Lin
We present a miniature oblique back-illumination microscope (mOBM) for imaging the microcirculation of human oral mucosa, enabling real-time, label-free phase contrast imaging of leukocyte rolling and adhesion, the initial steps in leukocyte recruitment that is a hallmark of inflammation. Imaging cell motion can provide new diagnostic information (time course of disease progression, response to therapy
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Characteristics and Prognosis of HHV8-Positive Diffuse Large B-Cell Lymphoma, Not Otherwise Specified: A Population-Based Study medRxiv. Hematol. Pub Date : 2023-07-18 Fan Wang
Background: HHV8-positive diffuse large B-cell lymphoma, not otherwise specified (HDN) is a rare and aggressive lymphoma that usually arises in association with HHV8-positive multicentric Castleman disease. The epidemiology, treatment patterns and survival outcomes of HDN are poorly understood. Methods: A retrospective analysis was performed for 67 patients with HDN diagnosed from 2011 to 2020 using
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Label-free cell detection of acute leukemia using ghost cytometry medRxiv. Hematol. Pub Date : 2023-07-16 Yoko Kawamura, Kayoko Nakanishi, Yuri Murata, Kazuki Teranishi, Ryusuke Miyazaki, Keisuke Toda, Toru Imai, Yasuhiro Kajiwara, Keiji Nakagawa, Hidemasa Matsuo, Souichi Adachi, Sadao Ota, Hidefumi Hiramatsu
Early diagnosis and prompt initiation of appropriate treatment are critical for improving the prognosis of acute leukemia. Currently, acute leukemia is diagnosed by microscopic morphological examination of bone marrow smears and flow cytometric immunophenotyping of bone marrow cells stained with fluorophore-conjugated antibodies. However, these diagnostic processes require trained professionals and
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Safety and protection of plasma donors: A scoping review and evidence (gap) map medRxiv. Hematol. Pub Date : 2023-07-14 Natalie Schroyens, Tine D'aes, Emmy De Buck, Susan Mikkelsen, Pierre Tiberghien, Katja van den Hurk, Christian Erikstrup, Veerle Compernolle, Hans Van Remoortel
Background and objectives: As part of a large-scale European project aiming to safely increase plasma collection in Europe, the current scoping review identifies the existing evidence (gaps) on adverse events (AEs) and other health effects in plasmapheresis donors, as well as factors that may be associated with such events/effects. Materials and methods: We searched 6 databases and 3 registries. Study
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Resolving heterogeneity in Diffuse Large B-cell Lymphoma using a comprehensive modular expression map medRxiv. Hematol. Pub Date : 2023-07-05 Matthew A Care, Daniel Painter, Sharon Barrans, Chulin Sha, Peter Johnson, Andrew Davies, Ming-Qing Du, Simon Crouch, Alexandra Smith, Eve Roman, Catherine Burton, Gina M Doody, David Westhead, Ulf Klein, Daniel Hodson, Reuben Tooze
Diffuse large B-cell lymphoma (DLBCL) is characterised by pronounced genetic and biological heterogeneity. Several partially overlapping classification systems exist developed from mutation, rearrangement or gene expression data. We apply a customised network analysis to nearly five thousand DLBCL cases to identify and quantify modules indicative of tumour biology. We demonstrate that network-level
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Application of Deep Learning Models into the Prediction of Interleukin-6 and -8 Cytokines in Sickle Cell Anemia Patients medRxiv. Hematol. Pub Date : 2023-06-28 Dylan Nguyen, Lukas Abraham, Ashesh Amatya
Interleukin-6 (IL6) and Interleukin-8 (IL8) are cytokines related to general immune function, but within Sickle Cell Anemia (SCA) patients, their overproduction tends to cause autoimmune reactions. These vital cytokines engage in the pathophysiology of SCA, but the extent to which they are associated with the genetics of the disease requires further exploration. This research paper seeks to further
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Multiomic Analysis Identifies a High-Risk Metabolic and TME Depleted Signature that Predicts Early Clinical Failure in DLBCL medRxiv. Hematol. Pub Date : 2023-06-10 Kerstin Wenzl, Matt Stokes, Joseph Novak, Allison Bock, Sana Khan, Melissa Hopper, Jordan Krull, Abigail Dropik, Janek Walker, Vivekananda Sarangi, Raphael Mwangi, Maria Ortiz, Nicholas Stong, Chris Huang, Matthew Maurer, Lisa Rimsza, Brian Link, Susan Slager, Yan Asmann, Patrizia Mondello, Ryan Morin, Stephen Ansell, Thomas Habermann, Andrew Feldman, Rebecca King, Grzegorz Nowakowski, James Cerhan
PURPOSE 60-70% of newly diagnosed diffuse large B-cell lymphoma (DLBCL) patients avoid events within 24 months of diagnosis (EFS24) and the remainder have poor outcomes. Recent genetic and molecular classification of DLBCL has advanced our knowledge of disease biology, yet were not designed to predict early events and guide anticipatory selection of novel therapies. To address this unmet need, we used
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Effects of plasmapheresis frequency on health status and exercise performance in men: a randomized controlled trial medRxiv. Hematol. Pub Date : 2023-06-05 Alexandre Mortier, Jina Khoudary, Sophie van Doorslaer de Ten Ryen, Camille Lannoy, Nicolas Benoit, Nancy Antoine, Sylvie Copine, Hans Van Remoortel, Philippe Vandekerckhove, Veerle Compernolle, Louise Deldicque
Objectives To collect data on 1) haematological and biochemical markers, 2) physiological and exercise-related parameters and 3) adverse events to get a comprehensive picture of the safety of intensive or less intensive plasma donation protocols.
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Use of Point-of-care Haemoglobin Tests to Diagnose Childhood Anaemia in Low-and Middle-Income Countries: A Systematic Review medRxiv. Hematol. Pub Date : 2023-06-04 Rebecca Brehm, Annabelle South, Elizabeth C George
Objectives Anaemia is a major cause of mortality and transfusion in children in Low- and Middle-Income Countries (LMICs), however current diagnostics are slow, costly, and frequently unavailable. Point-of-care haemoglobin tests (POC(Hb)Ts) could improve patient outcomes and use of resources by providing rapid and affordable results. We systematically reviewed the literature to investigate what, where
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Mortality from Sickle Cell Disease in Brazil medRxiv. Hematol. Pub Date : 2023-06-03 PF Blatyta, C DiLorenzo, I Gomes, T Salomon, EC Sabino, L Capuani, DTS Cruz, C Maximo, MV Flor-Park, RA Mota, DO Werneck Rodrigues, CL Dinardo, C Almeida-Neto, B Custer, S Kelly, Recipient Epidemiology and Donor Evaluation Study-III (REDS-III) International Component Brazil
Introduction Many individuals with sickle cell disease (SCD) die before age 60, despite early detection via neonatal screening and implementation of treatments such as vaccines and antibiotic prophylaxis and the increasing availability of disease modifying therapies. This study evaluated the causes and independent predictors of mortality in a SCD population in Brazil.
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Patients with sickle cell disease presented dysregulated plasma Rb/K ratio and Gamma-glutamyl cycle in red blood cells medRxiv. Hematol. Pub Date : 2023-05-26 Shruti Bhatt, Amit Kumar Mohapatra, Apratim Sai Rajesh, Satybrata Meher, Pradip Kumar Panda, RANJAN NANDA, Suman Kundu
Patients suffering from sickle cell disease (SCD) present with multifactorial pathology, and a detailed understanding of it may help to develop novel therapeutics. In this study, the plasma elemental (24Mg, 44Ca, 57Fe, 63Cu, 66Zn, 77Se, 85Rb, 208Pb, and 39K) levels of SCD patients (n=10, male: 50%) and control groups (trait and healthy; n=10 each; male: 50%) were profiled using inductively coupled
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Causal relationships between risk of venous thromboembolism and 18 cancers: a bidirectional Mendelian randomisation analysis medRxiv. Hematol. Pub Date : 2023-05-18 Naomi Cornish, Philip Haycock, Hermann Brenner, Jane C. Figueiredo, Tessel Galesloot, Robert C Grant, InterLymph consortium, INVENT-MVP consortium, Mattias Johansson, Daniela Mariosa, James McKay, Rish Pai, Andrew J Pellatt, Jewel Samadder N., Jianxin Shi, Florian Thibord, David-Alexandre Trégouët, Catherine Voegele, Chrissie Thirlwell, Andrew Mumford, Ryan Langdon
Background: People with cancer experience high rates of venous thromboembolism (VTE). Additionally, risk of subsequent cancer is increased in people experiencing their first VTE. The causal mechanisms underlying this association are not completely understood, and it is unknown whether VTE is itself a risk factor for cancer.
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Malaria Infection in Patients with Sickle Cell Disease in Nigeria: Association with Markers of Hyposplenism medRxiv. Hematol. Pub Date : 2023-05-14 Adama I Ladu, Mairo Y Kadaura, Mohammed Dauda, Abubakar Sadiq Baba, Nasir Garba Zango, Caroline Jeffery, Abubakar Farate, Adekunle Adekile, Imelda Bates
Background Malaria is considered an important cause of morbidity and mortality among people living with sickle cell disease (SCD). This has partly been attributed to the loss of splenic function that occurs early in the disease process. We aimed to study the prevalence of malaria infection among Nigerian SCD patients and explore the association with spleen size and function.