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Adjuvant effects of Saccharomyces cerevisiae in the treatment of experimental periodontitis in rats undergoing chemotherapy. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-11-17 Valdir Gouveia Garcia,Tiago Esgalha da Rocha,Natália Amanda Gomes,Daniela Maria Janjácomo Miessi,Marta Aparecida Alberton Nuernberg,João Victor Soares Rodrigues,Jânderson de Medeiros Cardoso,Edilson Ervolino,Letícia Helena Theodoro
Surgical procedures, radiotherapy, and chemotherapy, individually or in association, are current oncological treatments. Among the most used chemotherapy drugs, 5-fluorouracil (5FU) is an antimetabolite with a broad spectrum of action. This study evaluated the effects of probiotics (PRO) as an adjuvant to the treatment of experimental periodontitis (EP) in rats immunosuppressed with 5FU.108 rats were
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Biofilm dysbiosis and caries activity: a surface or an individual issue? J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-11-13 Laís Daniela Ev,Joice Faria Poloni,Nailê Damé-Teixeira,Rodrigo Alex Arthur,Daniela Jorge Corralo,Sandra Liana Henz,Thuy DO,Marisa Maltz,Clarissa Cavalcanti Fatturi Parolo
OBJECTIVE This study aimed to analyze the functional profile of supragingival biofilm from sound (CAs), active (CAa), and inactive (CAi) enamel caries lesions from caries-active individuals to provide insights into the diversity of biological processes regarding biofilm dysbiosis. METHODOLOGY A metatranscriptome analysis was performed in biofilm samples collected from five caries-active individuals
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Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-11-13 Anupong Thongklam Songsaad,Sarut Thairat,Peeratchai Seemaung,Amarin Thongsuk,Tatcha Balit,Nisarat Ruangsawasdi,Chareerut Phruksaniyom,Thanasup Gonmanee,Kenneth L White,Charoensri Thonabulsombat
OBJECTIVES The endogenous repairing based on the activation of neural stem cells (NSCs) is impaired by neurodegenerative diseases. The present study aims to characterize human stem cells from the apical papilla (hSCAPs) with features of mesenchymal stem cells (MSCs) and to demonstrate the neuronal differentiation of hSCAPs into NSCs through the formation of three-dimensional (3D) neurospheres, verifying
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Effects of silver diamine fluoride on demineralization protection after a secondary acid challenge. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-11-03 Mauro A Tudares,George J Eckert,Frank Lippert
OBJECTIVE This investigation describes the effects of 5% sodium fluoride varnish and 38% silver diamine fluoride on demineralization protection of human enamel lesions of three different severities after a secondary acid challenge. STUDY DESIGN Specimens underwent color and enamel surface microhardness change measurements after demineralization and treatment events. Transverse microradiography was
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Effects of systemic Anatolian propolis administration on a rat-irradiated osteoradionecrosis model. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-27 Sefa Çolak,Aras Erdil,Fikret Gevrek
OBJECTIVE Radiotherapy after head and neck cancer is associated with the risk of osteonecrosis development. This study aims to investigate the effectiveness of systemic propolis application to prevent the disease as it has no definite treatment protocol despite the proposed treatment methods and significantly decreases individuals' quality of life. METHODOLOGY In total, 29 male Wistar-Albino rats were
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Survival of severely compromised endodontically treated teeth restored with or without a fiber glass post. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-27 Maria Tereza Hordones Ribeiro,Gabriella de Oliveira,Helena Letícia Quirino de Oliveira,Lilibeth Carola Leyton Mendoza,Calebe de Melo,Thiago Silva Peres,Carlos José Soares
OBJECTIVE The use of a fiber glass post (FGP) type and choice of FGP diameter to restore endodontically treated incisors without ferrule is controversial. This study evaluated survival rate and failure mode of severely compromised central incisors without ferrule rehabilitated using resin-based composite (RBC) with or without FGP with different diameters. METHODOLOGY A total of 60 decoronated bovine
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Test-retest reliability of the isometric contraction test (IC test) of the masticatory muscles in subjects with and without temporomandibular muscle disorders. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-27 Marcos Iglesias-Peón,Juan Mesa-Jiménez,César Fernández-DE-Las-Peñas,Nuria García Iglesias,Carmen María Iglesias Peón,Daiana Priscila Rodrigues-DE-Souza,Francisco Alburquerque-Sendín
OBJECTIVE Recently, the DC/TMD has become an essential tool for the diagnosis of temporomandibular disorders (TMD). However, as they fail to include functional activities, new assessment proposals have emerged, such as the isometric contraction test (IC test) of the masticatory muscles, which uses muscle contractions to identify muscular TMD. This study aimed to determine the test-retest reliability
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In-office dental bleaching in adolescents using 6% hydrogen peroxide with different application tips: randomized clinical trial. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-27 Taynara de Souza Carneiro,Michael Willian Favoreto,Michel Wendlinger Cantanhede Ferreira,Laís Giacomini Bernardi,Heloísa Forville de Andrade,Matheus Coelho Bandeca,Alessandra Reis,Laura Ceballos García,Alessandro Dourado Loguercio
OBJECTIVE Despite the availability of in-office bleaching gels with a 6% concentration of hydrogen peroxide (HP), these gels have not been evaluated in younger patients. They are commercially available with a tip, associated or not with a brush, where the tip with a brush spreads the gel over the entire surface to have a smaller thickness (thin layer) since the manufacturers indicate the application
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Influence of the combined effect of desensitizing dentifrices and universal adhesives on dentin bond strength under erosive conditions. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-27 Fabiana Tavares Lunardi Palhari,Laís da Mata Almeida,Priscila Christiane Suzy Liporoni,Leandro Augusto Hilgert,Rayssa Ferreira Zanatta
OBJECTIVE This study aimed to evaluate whether the use of desensitizing dentifrices containing obliterating agents can affect bond strength of eroded/abraded dentin. METHODOLOGY A total of 100 dentin samples were obtained from human molars. The teeth were cut into 3 mm-thickness discs and allocated in five groups (n=20), according to the toothpaste used: WoF - abrasion with fluoride-free toothpaste
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Evaluation of the effect of Matricaria recutita monotherapy or in combination with photodynamic therapy on tissue repair in the dorsum of the tongue of rats. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-27 Juliana Borges de Lima Dantas,Tila Fortuna,Hortência Resende Della Cella,Fábio Luís Meneses de Sousa da Silva,Rejane Conceição Santana,Gabriela Botelho Martins
OBJECTIVE The search for treatments that accelerate the healing of lesions is of constant interest. Matricaria recutita (chamomile) is a plant with antimicrobial, anti-inflammatory, and healing properties, and antimicrobial Photodynamic Therapy (aPDT) eradicates microorganisms, which favors tissue repair. This study aimed to evaluate the effect of the topical use of chamomile with or without aPDT on
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Influence of daily usage times on patients' compliance during at-home bleaching: a randomized clinical trial. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-09 Caio César Pavani,Ticiane Cestari Fagundes,Daniel Sundfeld,Gabriela Cristina Santin,Lucas Silveira Machado,André Pinheiro de Magalhães Bertoz,Timm Cornelius Schott,Renato Herman Sundfeld
The effectiveness of at-home dental bleaching treatments depends on the time that bleaching products are in contact with the teeth surface and, consequently, on the adequate use of associated custom acetate trays. OBJECTIVE This randomized single-blinded trial aimed to analyze if the daily usage time of these products influences the patient's compliance behavior when submitted to monitored at-home
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Anticancer effects of gossypetin from Hibiscus sabdariffa in oral squamous cell carcinoma. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-09 Ke Huang,Zhibin Liu,Myoung-Ok Kim,Ki-Rim Kim
OBJECTIVE Gossypetin, isolated from Hibiscus sabdariffa L, has been shown to have various pharmacological effects including anti-inflammatory and antibacterial activity against various diseases. However, since the effect of gossypetin in oral cancer remains to be reported, we aimed to investigate the anticancer activity and mechanisms of gossypetin in oral squamous cell carcinoma (OSCC). METHODOLOGY
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Interleukin-17 plays a role in dental pulp inflammation mediated by zoledronic acid: a mechanism unrelated to the Th17 immune response? J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-09 Anna Clara Aragão Matos Carlos,José Vitor Mota Lemos,Marcela Maria Fontes Borges,Maria Carolina Portela Albuquerque,Fabrício Bitu Sousa,Ana Paula Negreiros Nunes Alves,Thinali Sousa Dantas,Paulo Goberlânio de Barros Silva
OBJECTIVE To evaluate the influence of RORγT inhibition by digoxin on inflammatory changes related to interleukin-17 (IL-17) in the pulp of rats treated with zoledronate (ZOL). METHODOLOGY Forty male Wistar rats were divided into a negative control group (NCG) treated with saline solution, a positive control group (PCG) treated with ZOL (0.20 mg/kg), and three groups treated with ZOL and co-treated
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Divergence between confidence and knowledge of endodontists regarding non-odontogenic pain. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-09 Marcos Dezotti Luiz,Letycia Accioly Simões Coelho,Rodrigo Ricci Vivan,Marco Antônio Hungaro Duarte,Murilo Priori Alcalde,Paulo César Rodrigues Conti,Yuri Martins Costa,Leonardo Rigoldi Bonjardim
INTRODUCTION This study aimed to assess the self-reported levels of confidence and knowledge related to non-odontogenic pain among a group of Brazilian endodontists. METHODOLOGY A total of one hundred and forty-six endodontists affiliated with the Brazilian Society of Endodontics participated in the survey. The questionnaire, distributed via email or WhatsApp, contained inquiries designed to gauge
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Survival rate of the Hall technique compared with resin composite restoration in multi-surface cavities in primary teeth: a 1-year randomized clinical trial. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-10-09 Aline Maquiné Pascareli-Carlos,Tamara Kerber Tedesco,Ana Flávia Bissoto Calvo,Isabela Floriano,Thais Gimenez,Monicque da Silva Gonçalves,Daniela Calumby,José Carlos Pettorossi Imparato
BACKGROUND Hall technique (HT) has been indicated for teeth with dentinal caries lesion; however, extensive cavities, with more than two surfaces still seem challenging for restorative treatment in pediatric dentistry, resulting in a higher failure rate and an increased need for retreatment. OBJECTIVES To compare the survival rate of the Hall technique preformed metal crown (HT) with resin composite
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Investigating the association between dental age and polymorphisms in genes encoding estrogen receptors. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-25 Isabela Ribeiro Madalena,Caio Luiz Bitencourt Reis,Mirian Aiko Nakane Matsumoto,Maria Bernadete Sasso Stuani,Natanael Henrique Ribeiro Mattos,Daniela Silva Barroso de Oliveira,Maria Angélica Hueb de Menezes Oliveira,Liliane Roskamp,Erika Calvano Küchler,Flares Baratto-Filho
BACKGROUND Genetic polymorphisms have been shown to influence several physiological traits, including dental and craniofacial characteristics. Understanding the clinical relevance of genetic polymorphisms in dental practice is crucial to personalize treatment plans and improve treatment outcomes. OBJECTIVE to evaluate the association between dental age and genetic polymorphisms in genes encoding estrogen
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Association between nutritional status and children and adolescents' dental caries experiences: an overview of systematic reviews. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-25 Thaís de Oliveira Fernandes,Patrícia Arriaga Carvalho,Fernanda Volpe de Abreu,Christian Kirschneck,Erika Calvano Küchler,Leonardo Santos Antunes,Lívia Azeredo Alves Antunes
An increasing number of systematic reviews (SR) has investigated the association between dental caries and nutritional status in children and adolescents, thus requiring an overview to compile the information in a single piece of evidence. Therefore, this study aimed to evaluate and summarize evidence from published SR on the association between dental caries and nutritional status in children and
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Obesity influences the development of bisphosphonate-induced osteonecrosis in Wistar rats. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-25 Wilson José de Miranda Lima,Jannerson Cesar Xavier de Pontes,Ludmila Silva de Figueiredo,Rubens da Silva Araújo,Maria Carolina de Paiva Sousa,Jailane de Souza Aquino,Ricardo Dias de Castro,Adriano Francisco Alves
Medication-related osteonecrosis of the jaw (MRONJ) is characterized by bone exposure for more than eight weeks in patients who have used or been treated with antiresorptive or antiangiogenic drugs, without a history of radiation therapy or metastatic diseases in the jaws. Obesity is associated with changes in periodontal tissues and oral microbiota that are linked to bone alterations. This study aimed
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Colonization dynamics of subgingival microbiota in recently installed dental implants compared to healthy teeth in the same individual: a 6-month prospective observational study. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-15 Carina Maciel Silva-Boghossian,Pablo Torquilho Duarte,Denise Gome da Silva,Talita Gomes Baêta Lourenço,Ana Paula Vieira Colombo
OBJECTIVES To evaluate the colonization dynamics of subgingival microbiota established over six months around newly installed dental implants in periodontally healthy individuals, compared with their corresponding teeth. METHODOLOGY Seventeen healthy individuals assigned to receive single dental implants participated in the study. Subgingival biofilm was sampled from all implant sites and contralateral/
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Does radiation therapy affect adhesion of tricalcium silicate cements to root dentin? J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-15 Lochan Khullar,Nidambur Vasudev Ballal,Tan Fırat Eyüboğlu,Mutlu Özcan
OBJECTIVE This study aimed to analyze the effect of irradiation on the push-out bond strength of mineral trioxide aggregate (MTA) and Biodentine to radicular dentin. METHODOLOGY A total of 60 extracted mature human teeth with single root canals were categorized into two groups (irradiated and non-irradiated) (n=30). Each group was further divided into two sub-groups based on cements used (Biodentine
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Chitosan microparticles loaded with essential oils inhibit duo-biofilms of Candida albicans and Streptococcus mutans. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-15 Lana Glerieide Silva Garcia,Maria Gleiciane da Rocha,Rosemayre Souza Freire,Paulo Iury Gomes Nunes,João Victor Serra Nunes,Mirele Rodrigues Fernandes,Waldemiro Aquino Pereira-Neto,José Júlio Costa Sidrim,Flavia Almeida Santos,Marcos Fábio Gadelha Rocha,Lidiany Karla Azevedo Rodrigues,Rodrigo Silveira Vieira,Raimunda Sâmia Nogueira Brilhante
OBJECTIVE Oral candidiasis is a common fungal infection that affects the oral mucosa, and happens when Candida albicans interacts with bacteria in the oral microbiota, such as Streptococcus mutans, causing severe early childhood caries. C. albicans and S. mutans mixed biofilms are challenging to treat with conventional antimicrobial therapies, thus, new anti-infective drugs are required. This study
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Dental anomalies in individuals with osteogenesis imperfecta: a systematic review and meta-analysis of prevalence and comparative studies. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-09-04 Heloisa Vieira Prado,Enio Cássio Barreto Soares,Natália Cristina Ruy Carneiro,Ivanete Cláudia de Oliveira Vilar,Lucas Guimarães Abreu,Ana Cristina Borges-Oliveira
BACKGROUND Osteogenesis imperfecta (OI) is a rare genetic disorder primarily caused by mutations in the genes involved in the production of type 1 collagen. OI is also known as brittle bone disease. OBJECTIVE This study aims to describe the prevalence of dental anomalies (except dentinogenesis imperfecta) in individuals with OI, and compare the prevalence of dental anomalies between individuals with
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Pro-angiogenic photo-crosslinked silk fibroin hydrogel: a potential candidate for repairing alveolar bone defects. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-08-28 Siyuan Wu,Xuezhong Zhou,Yilong Ai
OBJECTIVE This study aimed to develop a pro-angiogenic hydrogel with in situ gelation ability for alveolar bone defects repair. METHODOLOGY Silk fibroin was chemically modified by Glycidyl Methacrylate (GMA), which was evaluated by proton nuclear magnetic resonance (1H-NMR). Then, the photo-crosslinking ability of the modified silk fibroin was assessed. Scratch and transwell-based migration assays
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Trigger point dry needling increases masseter muscle oxygenation in patients with temporomandibular disorder. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-08-25 Carolina Ferreira de Macedo,Anelise Sonza,Alexia Nadine Puel,Adair Roberto Dos Santos
BACKGROUND Temporomandibular disorder (TMD) is an umbrella term encompassing various clinical complaints involving the temporomandibular joints, masticatory muscles, and/or associated orofacial structures. Myogenous TMDs are the most frequent cause of chronic orofacial pain. Musculoskeletal pain is commonly associated with myofascial trigger points (MTPs), for which dry needling (DN) is a routine treatment
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A systematic review and meta-analysis of the clinical performance of implant-supported overdentures retained by CAD-CAM bars. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-08-25 Ana Paula Chappuis-Chocano,Helena Sandrini Venante,Rodrigo Moreira Bringel da Costa,Mariana Domingues Pordeus,Oscar Oswaldo Marcillo-Toala,Joel Ferreira Santiago Junior,Vinícius Carvalho Porto
Currently, there is no consensus on the indications and clinical performance of implant-supported overdentures (IODs) involving computer-aided design and manufacturing (CAD-CAM) bars. OBJECTIVE To evaluate the performance of IODs involving CAD-CAM bars. METHODOLOGY A comprehensive search of studies published until May 2023 was conducted in many databases, including PubMed/MEDLINE, Web of Science, Cochrane
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Rinsing solutions containing natural extracts and fluoride prevent enamel erosion in vitro. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-24 Tommy Baumann,Samira Helena Niemeyer,Adrian Lussi,Taís Scaramucci,Thiago Saads Carvalho
BACKGROUND Polyphenols interact with salivary proteins and thus can improve the pellicle's erosion protective properties. This effect could be exploited to create rinsing solutions with polyphenols as active ingredients for erosion prevention. Different from the current gold standard for erosion protective rinsing solutions, these rinses would not rely on stannous ions. This would offer alternatives
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Chronic exposure to lipopolysaccharides as an in vitro model to simulate the impaired odontogenic potential of dental pulp cells under pulpitis conditions. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-24 Igor Paulino Mendes Soares,Caroline Anselmi,Maria Luiza Barucci Araujo Pires,Rafael Antonio de Oliveira Ribeiro,Maria Luísa Leite,Diana Gabriela Soares,Carlos Alberto DE Souza Costa,Josimeri Hebling
BACKGROUND Simulating a bacterial-induced pulpitis environment in vitro may contribute to exploring mechanisms and bioactive molecules to counteract these adverse effects. OBJECTIVE To investigate the chronic exposure of human dental pulp cells (HDPCs) to lipopolysaccharides (LPS) aiming to establish a cell culture protocol to simulate the impaired odontogenic potential under pulpitis conditions. METHODOLOGY
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Different concentrations of fetal bovine serum affect cytokine modulation in Lipopolysaccharide-activated apical papilla cells in vitro. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-24 Letícia Martins Santos,Patricia E Silva Cardoso,Elisa Abreu Diniz,Juliana Garuba Rahhal,Carla Renata Sipert
BACKGROUND Fetal bovine serum (FBS) is the most used supplement in culture media; however, it may interfere with in vitro assays via effects on cell proliferation and cytokine production. The ideal FBS concentration for assays using apical papilla cells (APCs) remains unknown. Therefore, this study aimed to evaluate the effects of FBS on APC activation, cell viability/proliferation, and cytokine production
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TIPE2 regulates periodontal inflammation by inhibiting NF-κB p65 phosphorylation. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-24 Yanmei DU,Xiaohua Liu,Changjie Xiao,Jianbin Li,Zhenxian Sheng,Yuxin Wang,Ronglin Wang,Xijiao Yu
BACKGROUND The roles and molecular mechanisms of tumor necrosis factor-α-induced protein 8-like 2 (TIPE2) in periodontitis remain largely unknown. OBJECTIVE This study aimed to determine the expression of TIPE2 and NF-κB p65 in rat Porphyromonas gingivalis-induced periodontics in vivo. METHODOLOGY Periodontal inflammation and alveolar bone resorption were analyzed using western blotting, micro-computed
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Association of rs142548867 (EEFSEC) and periodontitis Grade C in a young Brazilian population. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-17 Camila Schmidt Stolf,Tiago Taiete,Paloma A do Nascimento,Hélvis E S Paz,Enílson Antônio Sallum,Karina Gonzalez Silvério Ruiz,Márcio Zaffalon Casati,Renato Corrêa Viana Casarin
BACKGROUND Periodontitis Stage III-IV, Grade C (PerioC) is a severe form of Periodontitis. The individual genetic background has been shown to be an important etiopathogenic factor for the development of this disease in young, systemically healthy, and non-smokers patients. Recently, after exome sequencing of families with a history of the disease, PerioC was associated with three single nucleotide
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Effectiveness of disinfectant solutions associated or not with brushing on the biofilm control of a 3D printed-denture base resin. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-17 Thaís Soares Bezerra Santos Nunes,Marcela Dantas Dias da Silva,Sabrina Romão Gonçalves Coelho,Hamile Emanuella do Carmo Viotto,Ana Carolina Pero
BACKGROUND The formation of biofilm on denture bases is a recurrent clinical problem that favors the development of denture stomatitis. The effectiveness of a hygiene protocol in a 3D-printed denture base resin is still uncertain. OBJECTIVE To evaluate of the effectiveness of immersion, associated or not with brushing in a soap solution, on the biofilm control of a 3D-printed denture base resin. METHODOLOGY
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Feasible low bone density condition for assessing bioactivity in ex-in vivo and in vivo studies. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-07 William Phillip Pereira DA Silva,Leonardo Alan Delanora,Barbara Ribeiro Rios,Stéfany Barbosa,Maria Eloise de Sá Simon,Cortino Sukotjo,Leonardo P Faverani
OBJECTIVE To choose a critical animal model for assessments of bone repair with implant installation by comparing senile rats (SENIL) to young ovariectomized rats (OXV). METHODOLOGY For the ex-in vivo study, the femurs were precursors for bone marrow mesenchymal stem cells. Cellular responses were performed, including cell viability, gene expression of osteoblastic markers, bone sialoprotein immunolocalization
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Association between the Mediterranean Diet Index and self-reported Gingival Health Status Indicators in a population of Chilean adults: a cross-sectional study. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-07-03 Gustavo Sáenz-Ravello,Loreto Matamala,Patricia Cisternas,Jorge Gamonal,Patricia Hernández,Nidia Castro Dos Santos,Ke Deng,Mauricio Baeza
BACKGROUND Despite the recognized impact of diet on non-communicable diseases, the association between the Mediterranean diet and periodontal diseases is still uncertain. This study aimed to determine the association between adherence to the Mediterranean Diet Index (MDI) and self-reported gingival health status in Chilean adults, exploring the feasibility of using validated web-based survey questionnaires
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Fibroblasts at the curtain call: from ensemble to principal dancers in immunometabolism and inflammaging. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-06-23 Rogelio Serrano-Lopez,Ana Carolina Morandini
Inflammation is a necessary step in response to injuries, being vital in restoring homeostasis and facilitating tissue healing. Among the cells that play a crucial role in inflammatory responses, stromal cells, including fibroblasts, have an undeniable significance in fine-tuning the magnitude of mediators that directly affect hyper-inflammatory responses and tissue destruction. Fibroblasts, the dominant
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Clinical performance of an alkasite-based bioactive restorative in class II cavities: a randomized clinical trial. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-06-23 Fatma Dilsad Oz,Ece Meral,Sevil Gurgan
OBJECTIVE This clinical study aimed to evaluate the clinical performance of an alkasite-based bioactive material by comparing it with a resin composite (RC) in the restoration of Class II cavities over a year. METHODOLOGY A hundred Class II cavities were restored at 31 participants. Groups were as follows: Cention N (CN) (Ivoclar Vivadent, Schaan, Liechtenstein) and G-ænial Posterior (GP) (GC, Tokyo
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Effect of fluoride gels with nano-sized sodium trimetaphosphate on the in vitro remineralization of caries lesions. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-06-07 Mariana Emi Nagata,Alberto Carlos Botazzo Delbem,Liliana Carolina Báez-Quintero,Marcelle Danelon,Caio Sampaio,Douglas Roberto Monteiro,Annette Wiegand,Juliano Pelim Pessan
OBJECTIVE To evaluate the effects of fluoride (F) gels supplemented with micrometric or nano-sized sodium trimetaphosphate (TMPmicro and TMPnano, respectively) on the in vitro remineralization of caries-like lesions. METHODOLOGY Bovine enamel subsurface lesions (n=168) were selected according to their surface hardness (SH) and randomly divided into seven groups (n=24/group): Placebo (without F/TMP)
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Metabolic effect of low fluoride levels in the islets of NOD mice: integrative morphological, immunohistochemical, and proteomic analyses. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-06-05 Juliana Sanches Trevizol,Aline Dionizio,Aislan Quintiliano Delgado,Talita Mendes Oliveira Ventura,Caroline Fernanda da Silva Ribeiro,Laura Ribeiro,Nathalia Rabelo Buzalaf,Tânia Mary Cestari,Ana Carolina Magalhães,Maiko Suzuki,José Roberto Bosqueiro,Marília Afonso Rabelo Buzalaf
OBJECTIVES Fluoride (F) has been widely used to control dental caries, and studies suggest beneficial effects against diabetes when a low dose of F is added to the drinking water (10 mgF/L). This study evaluated metabolic changes in pancreatic islets of NOD mice exposed to low doses of F and the main pathways altered by the treatment. METHODOLOGY In total, 42 female NOD mice were randomly divided into
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An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-06-05 Nutthapong Kantrong,Jittranut Kumtawee,Teerasak Damrongrungruang,Subin Puasiri,Anupong Makeudom,Suttichai Krisanaprakornkit,Pattama Chailertvanitkul
OBJECTIVE To explore the potential for development of Thai propolis extract as a pulp capping agent to suppress pulpal inflammation from dental pulp infections. This study aimed to examine the anti-inflammatory effect of the propolis extract on the arachidonic acid pathway, activated by interleukin (IL)-1β, in cultured human dental pulp cells. METHODOLOGY Dental pulp cells, isolated from three freshly
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Effect of anaerobic or/and microaerophilic atmosphere on microcosm biofilm formation and tooth demineralization. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-06-01 Aline Silva Braga,Kim Rafaela Ricci,Ana Carolina Magalhães
OBJECTIVE Microcosm biofilms can reproduce the complexity of a dental biofilm. However, different forms of cultivation have been used. The impact of the culture atmosphere on the development of microcosm biofilms and their potential to cause tooth demineralization has not yet been deeply studied. This study analyzes the effects of three experimental cultivation models (microaerophile vs. anaerobiosis
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Effect of genetic polymorphisms rs2301113 and rs2057482 in the expression of HIF-1α protein in periodontal ligament fibroblasts subjected to compressive force. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-29 Erika Calvano Küchler,Vinicius Broska Teodoro,Agnes Schröder,Ute Nazet,Michelle Nascimento Meger,Patricia Valéria Manozzo Kunz,Flares Baratto-Filho,Gerrit Spanier,Rafaela Scariot,Peter Proff,Christian Kirschneck
OBJECTIVE Many genes and signaling molecules are involved in orthodontic tooth movement, with mechanically and hypoxically stabilized HIF-1α having been shown to play a decisive role in periodontal ligament signaling during orthodontic tooth movement. Thus, this in vitro study aimed to investigate if genetic polymorphisms in HIF1A (Hypoxia-inducible factor α-subunits) influence the expression pattern
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Final irrigation protocols can be used to promote stable long-term bond strength of AH Plus to dentin. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-26 Talita Tartari,Caroline Wichnieski,Renato Menezes Silva,Ariadne Letra,Marco Antonio Hungaro Duarte,Clovis Monteiro Bramante
Irrigation solutions might affect dentin surface characteristics and, consequently, endodontic sealers adhesion. This study analyzed the effect of different final irrigation protocols on push-out bond strength (BS) of AH Plus to dentin seven days and 20 months after obturation. Scanning electron micrographs were obtained from the dentin surface of one sample/group after final irrigation. Canals of
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Characterization of whitening toothpastes and their effect on the physical properties of bulk-fill composites. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-15 Raíssa Manoel Garcia,Waldemir Francisco Vieira Junior,Danielle Ferreira Sobral-Souza,Flávio Henrique Baggio Aguiar,Débora Alves Nunes Leite Lima
To characterize activated charcoal and 2% hydrogen peroxide-based toothpastes and investigate their effects on roughness, color change, and gloss of bulk-fill composite resin. Composite resin specimens (Aura Bulk Fill, SDI) were subjected to 5,000 brushing cycles with regular toothpaste (Colgate Total 12, [RT]), activated charcoal toothpaste (Bianco Dental Carbon, [AC]), or hydrogen peroxide-containing
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Salivary protein candidates for biomarkers of oral disorders in people with a crack cocaine use disorder. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-15 Cassiano Lima Chaiben,Nayara Flores Macedo,Thiago Beltrami Dias Batista,Carlos Antonio Schaffer Penteado,Talita M O Ventura,Aline Dionizio,Paulo Henrique Couto Souza,Marília Afonso Rabelo Buzalaf,Luciana Reis Azevedo-Alanis
The use of cocaine and its main derivative, crack, can cause some systemic effects that may lead to the development of some oral disorders. To assess the oral health of people with a crack cocaine use disorder and identify salivary protein candidates for biomarkers of oral disorders. A total of 40 volunteers hospitalized for rehabilitation for crack cocaine addiction were enrolled; nine were randomly
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Diagnostic potential of Type VII Collagen during oral carcinogenesis. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-15 Sopee Poomsawat,Abdulshukor Kariya,Thirayost Nimmanon,Theerachai Kosanwat,Rachai Juengsomjit,Sanguansin Sirima
Type VII collagen (Col7) is a major component of anchoring fibrils. Col7 plays a role in tumor development and aggressiveness of cutaneous squamous cell carcinoma of recessive dystrophic epidermolysis bullosa. However, the role of Col7 in oral squamous cell carcinoma (OSCC) and oral leukoplakia (OL) remains largely unknown. To elucidate the role of Col7 and its diagnostic potential during oral carcinogenesis
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Immunoexpression profile of hypoxia-inducible factor (HIF) targets in potentially malignant and malignant oral lesions: a pilot study. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-15 Shakiba Gholami,Cintia Chamorro-Petronacci,Mario Pérez-Sayáns,José Suárez Peñaranda,Adhemar Longatto-Filho,Fátima Baltazar,Julieta Afonso
Oral potentially malignant disorders (OPMD) are associated with an increased risk of oral squamous cell carcinoma (OSCC). OSCC has an aggressive profile and is the most prevalent among different head and neck malignancies. Most OSCC patients are diagnosed with advanced stage tumors and have a poor prognosis. Cancer cells are able to reprogram their metabolism, even in the presence of oxygen, enhancing
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Do matrix metalloproteinase and cathepsin K inhibitors work synergistically to reduce dentin erosion? J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-08 Xiujiao Lin,Xinwen Tong,Hui Yang,Yiying Chen,Hao Yu
OBJECTIVES To evaluate the effects of matrix metalloproteinase (MMP) and cathepsin K (catK) inhibitors on resistance to dentin erosion. METHODOLOGY A total of 96 dentin specimens (3×3×2 mm) were prepared and randomly assigned into four groups (n=24): deionized water (DW); 1 µM odanacatib (ODN, catK inhibitor); 1 mM 1,10-phenanthroline (PHEN, MMP inhibitor); and 1 µM odanacatib + 1 mM 1,10-phenanthroline
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Suppressing STAT3 activation impairs bone formation during maxillary expansion and relapse. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-08 Xiaoyue Xiao,Jianwei Chen,Qiming Zhai,Liangjing Xin,Xinhui Zheng,Si Wang,Jinlin Song
OBJECTIVES The mid-palatal expansion technique is commonly used to correct maxillary constriction in dental clinics. However, there is a tendency for it to relapse, and the key molecules responsible for modulating bone formation remain elusive. Thus, this study aimed to investigate whether signal transducer and activator of transcription 3 (STAT3) activation contributes to osteoblast-mediated bone
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Outcomes of permanent canines on the cleft side after secondary alveolar grafting using different materials in complete unilateral cleft lip and palate. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-01 Bruno Mariano Ribeiro Braga,Claudia Resende Leal,Roberta Martinelli Carvalho,Gisele da Silva Dalben,Terumi Okada Ozawa
The prevalence of impaction of the permanent canine on the cleft side (PCCS) ranges from 12-35% after alveolar bone grafting (ABG). PCCSs usually develop above other permanent teeth in the alveolar process, gradually becoming vertical until they reach the occlusal plane. The type of cleft, hypodontia of lateral incisor on the cleft side, slower PCCS root development, and genetic factors are predictors
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Accuracy of two methods to detect the presence of halitosis: the volatile sulfur compounds concentration in the mouth air and the information from a close person. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-05-01 Nádia Cristina Pinheiro Rodrigues,Alexandre Abrão Neto,Paulo Nadanovsky
This study aimed to analyze the accuracy of two methods for detecting halitosis, the organoleptic assessment by a trained professional (OA) with volatile sulfur compounds (VSC) measurement via Halimeter® (Interscan Corporation) and information obtained from a close person (ICP). Participants were patients and companions who visited a university hospital over one year period to perform digestive endoscopy
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Degradable hydrogel fibers encapsulate and deliver metformin and periodontal ligament stem cells for dental and periodontal regeneration. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-04-28 Jingyao Yin,Qian Lei,Xinghong Luo,Tao Jiang,Xianghui Zou,Abraham Schneider,Hockin H K Xu,Liang Zhao,Dandan Ma
Human periodontal ligament stem cells (hPDLSCs) are promising cells for dental and periodontal regeneration. This study aimed to develop novel alginate-fibrin fibers that encapsulates hPDLSCs and metformin, to investigate the effect of metformin on the osteogenic differentiation of hPDLSCs, and to determine the regulatory role of the Shh/Gli1 signaling pathway in the metformin-induced osteogenic differentiation
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Dental and composite resin discoloration induced by different hydraulic calcium silicate-based cements: two-year in vitro assessment. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-04-28 Lucas Santos de Jesus,Tiago Lopes Dos Santos Reis,Bernardo Guerra Mendonça de Melo Machado,Ana Luísa Monteiro da Costa,Julia Menezes Savaris,Claudia Angela Maziero Volpato,Eduardo Antunes Bortoluzzi,Cleonice da Silveira Teixeira,Paula Cristina Dos Santos Vaz Fernandes,Lucas da Fonseca Roberti Garcia
Few long-term studies assess the discoloration induced by hydraulic calcium silicate-based cement on dental structures. In addition, as far as we know, no long-term study has assessed the discoloration induced by these cement on composite resin. This in vitro study aimed to assess, during a period of two years, the discoloration potential of different hydraulic calcium silicate-based cements (hCSCs)
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Mesenchymal stromal cells derived from exfoliated deciduous teeth express neuronal markers before differentiation induction. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-04-14 Letícia Fracaro,Agner Henrique Dorigo Hochuli,Ana Helena Selenko,Luiz Guilherme Achcar Capriglione,Paulo Roberto Slud Brofman,Alexandra Cristina Senegaglia
OBJECTIVE This study aimed to evaluate neuronal markers in stromal cells from human exfoliated deciduous teeth (SHED) and standardize the isolation and characterization of those cells. METHODOLOGY Healthy primary teeth were collected from children. The cells were isolated by enzymatic digestion with collagenase. By following the International Society for Cell and Gene Therapy (ISCT) guidelines, SHED
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Stem cell-derived exosomes from human exfoliated deciduous teeth promote angiogenesis in hyperglycemic-induced human umbilical vein endothelial cells. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-04-07 Thanapat Sunartvanichkul,Tawepong Arayapisit,Sujiwan Seubbuk Sangkhamanee,Chaiyapol Chaweewannakorn,Kengo Iwasaki,Phatchanat Klaihmon,Hathaitip Sritanaudomchai
OBJECTIVE To investigate the angiogenesis in human umbilical vein endothelial cells (HUVEC) under high glucose concentration, treated with exosomes derived from stem cells from human exfoliated deciduous teeth (SHED). METHODOLOGY SHED-derived exosomes were isolated by differential centrifugation and were characterized by nanoparticle tracking analysis, transmission electron microscopy, and flow cytometric
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In vitro effect of low-fluoride toothpaste supplemented with sodium trimetaphosphate, xylitol, and erythritol on enamel demineralization. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-31 Letícia Gonçalves Oliveira,Alberto Carlos Botazzo Delbem,Francyenne Maira Castro Gonçalves,Gabriela Leal Peres Fernandes,Mark L Cannon,Marcelle Danelon
OBJECTIVE Regular use of toothpaste with fluoride (F) concentrations of ≥ 1000 ppm has been shown to contribute to reducing caries increment. However, when used by children during the period of dental development, it can lead to dental fluorosis. In this study, we aimed to evaluate the in vitro effect of a toothpaste formulation with reduced fluoride (F) concentration (200 ppm) supplemented with sodium
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Absence of evidence is not evidence of absence. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-27 Magda Feres,Murilo Fernando Neuppmann Feres
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Human umbilical cord mesenchymal stem cells accelerate and increase implant osseointegration in diabetic rats. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-27 Mefina Kuntjoro,Nike Hendrijantini,Eric Priyo Prasetyo,Djoko Legowo,Ratri Maya Sitalaksmi,Bambang Agustono,Muhammad Dimas Aditya Ari,Guang Hong
OBJECTIVE This study was conducted to assess the effect of hUCMSCs injection on the osseointegration of dental implant in diabetic rats via Runt-related Transcription Factor 2 (Runx2), Osterix (Osx), osteoblasts, and Bone Implant Contact (BIC). METHODOLOGY The research design was a true experimental design using Rattus norvegicus Wistar strain. Rattus norvegicus were injected with streptozotocin to
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Synergistic antimicrobial potential of EGCG and fosfomycin against biofilms associated with endodontic infections. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-27 Cristiane Duque,Amanda Caselato Andolfatto Souza,Kelly Limi Aida,Jesse Augusto Pereira,Karina Sampaio Caiaffa,Vanessa Rodrigues Dos Santos,Leopoldo Cosme-Silva,Anuradha Prakki
OBJECTIVE This study aimed to evaluate the cytotoxicity and synergistic effect of epigallocatechin gallate (EGCG) and fosfomycin (FOSFO) on biofilms of oral bacteria associated with endodontic infections. METHODOLOGY This study determined minimum inhibitory and bactericidal concentration (MIC/MBC) and fractionated inhibitory concentration (FIC) of EGCG and FOSFO against Enterococcus faecalis, Actinomyces
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Evaluation of the remineralizing capacity of silver diamine fluoride on demineralized dentin under pH-cycling conditions. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-27 Carolina Cecilia Cifuentes-Jiménez,María Victoria Bolaños-Carmona,Tattiana Enrich-Essvein,Santiago González-López,Pedro Álvarez-Lloret
OBJECTIVE (1) to determine the effects of the silver diamine fluoride (SDF) and sodium fluoride (NaF) in demineralized dentin exposed to an acid challenge by pH-cycling, (2) to evaluate the remineralizing capacity of SDF/NaF products based on the physicochemical and mechanical properties of the treated dentin surfaces. METHODOLOGY In total, 57 human molars were evaluated in different stages of the
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Can high pain intensity and catastrophizing interfere with the cognitive performance of women with chronic pain related TMD? A cross-sectional study. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-27 Melissa de Oliveira Melchior,Luiza Guilherme Antunes,César Bataglion,Laís Valencise Magri
OBJECTIVE Interventions based on pain education and self-management are dependent on factors such as attention, memory, concentration, and executive function. To explore the relationship between cognitive performance and the variables pain intensity, central sensitization, catastrophizing, and hypervigilance in women diagnosed with chronic pain-related TMD. METHODOLOGY This is a cross-sectional study
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Microtomographic, histomorphometric, and molecular features show a normal alveolar bone healing process in iNOS-deficient mice along a compensatory upregulation of eNOS and nNOS isoforms. J. Appl. Oral Sci. (IF 2.7) Pub Date : 2023-03-20 Carolina Fávaro Francisconi,Priscila Maria Colavite,Angélica Cristina Fonseca,Michelle de Campos Soriani Azevedo,André Petenuci Tabanez,Jéssica Lima Melchiades,Andreia Espíndola Vieira,Carlos Eduardo Palanch Repeke,Marcela Claudino,Gustavo Pompermaier Garlet
METHODOLOGY Inducible nitric oxide synthase (iNOS) is one of the enzymes responsible for the synthesis of nitric oxide (NO), which is an important signaling molecule with effects on blood vessels, leukocytes, and bone cells. However, the role of iNOS in alveolar bone healing remains unclear. This study investigated the role of iNOS in alveolar bone healing after tooth extraction in mice. C57Bl/6 wild