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Genetic characterization of glyoxalase pathway in oral streptococci and its contribution to interbacterial competition J. Oral Microbiol. (IF 4.5) Pub Date : 2024-03-03 Lin Zeng, Payam Noeparvar, Robert A. Burne, Benjamin S. Glezer
To analyze contributions to microbial ecology of Reactive Electrophile Species (RES), including methylglyoxal, generated during glycolysis.Genetic analyses were performed on the glyoxalase pathway ...
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Characterization of pathogenic microbiome on removable prostheses with different levels of cleanliness using 2bRAD-M metagenomic sequencing J. Oral Microbiol. (IF 4.5) Pub Date : 2024-02-22 Tong Wah Lim, Shi Huang, Yuesong Jiang, Yufeng Zhang, Michael Francis Burrow, Colman McGrath
The microbiomes on the surface of unclean removable prostheses are complex and yet largely underexplored using metagenomic sequencing technology.To characterize the microbiome of removable prosthes...
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The effects of nitrate on the oral microbiome: a systematic review investigating prebiotic potential J. Oral Microbiol. (IF 4.5) Pub Date : 2024-02-27 Siobhan P. Moran, Bob T. Rosier, Fiona L. Henriquez, Mia C. Burleigh
Nitrate (NO3−) has been suggested as a prebiotic for oral health. Evidence indicates dietary nitrate and nitrate supplements can increase the proportion of bacterial genera associated with positive...
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Ecological competition in the oral mycobiome of Hispanic adults living in Puerto Rico associates with periodontitis J. Oral Microbiol. (IF 4.5) Pub Date : 2024-02-21 Kimil Acosta-Pagán, Benjamín Bolaños-Rosero, Cynthia Pérez, Ana P. Ortíz, Filipa Godoy-Vitorino
Background: Fungi are a major component of the human microbiome that only recently received attention. The imbalance of indigenous fungal communities and environmental fungi present in the oral cav...
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Secretome analysis and virulence assessment in Abiotrophia defectiva J. Oral Microbiol. (IF 4.5) Pub Date : 2024-02-12 Radhika G Bhardwaj, Mai E Khalaf, Maribasappa Karched
Abiotrophia defectiva, although infrequently occurring, is a notable cause of culture-negative infective endocarditis with limited research on its virulence. Associated with oral infections such as...
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Manipulating the diseased oral microbiome: the power of probiotics and prebiotics J. Oral Microbiol. (IF 4.5) Pub Date : 2024-01-31 X. Yu, D.A. Devine, J.J. Vernon
Dental caries and periodontal disease are amongst the most prevalent global disorders. Their aetiology is rooted in microbial activity within the oral cavity, through the generation of detrimental ...
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Antimicrobial effect of probiotic bacteriocins on Streptococcus mutans biofilm in a dynamic oral flow chamber model – an in vitro study J. Oral Microbiol. (IF 4.5) Pub Date : 2024-01-28 Elisabeth Reichardt, Viktoriya Shyp, Lea Alig, Carlalberta Verna, Eva M. Kulik, Michael M. Bornstein
To determine the antimicrobial activity of the bacteriocin-producing probiotic strains Streptococcus salivarius K12 and Streptococcus salivarius M18 alone or in combination against caries-associate...
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Effective disinfecting of negative pressure pipelines of DCUs reduces the risk of cross infection in dental care J. Oral Microbiol. (IF 4.5) Pub Date : 2024-01-07 Xuerong Lv, Ke Yan, Xiang Han, Feiyang Wang, Yuzhuo Ma, Shougang Zhang, Xiaoqian Wang
Microbial contamination of various accessory parts of the dental chair units (DCUs) is an essential source of cross infection, while the accessories of the crucial suction function are usually over...
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N-acyl homoserine lactones lactonase est816 suppresses biofilm formation and periodontitis in rats mediated by Aggregatibacter actinomycetemcomitans J. Oral Microbiol. (IF 4.5) Pub Date : 2024-01-07 Zelda Ziyi Zhao, Junmin Wang, Xinpai Liu, Zezhi Wang, Xianyu Zheng, Wuli Li, Tianfan Cheng, Jing Zhang
The current study aimed to explore the adjuvant therapeutic effect of N-acyl homoserine lactones (AHLs)-lactonase est816 on Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) biologic...
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Detection of Epstein-Barr virus infection in primary junctional epithelial cell cultures J. Oral Microbiol. (IF 4.5) Pub Date : 2024-01-03 Lilit Tonoyan, Charles V Olivieri, Marlène Chevalier, Robert Marsault, Alain Doglio
Junctional epithelium (JE) provides the front-line defense against pathogens invading periodontium. The breakdown of the JE barrier is the hallmark of periodontitis. Recent studies have implicated ...
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The importance of biofilm contamination control for dental unit waterlines: a multicenter assessment of the microbiota diversity of biofilm in dental unit waterlines J. Oral Microbiol. (IF 4.5) Pub Date : 2023-12-30 Piaopiao Chen, Jiang Zeng, Feiruo Hong, Cong Li, Huimin Wang, Xuefen Yu
The biofilm formation in Dental Unit Waterlines (DUWLs) could become an important cause of infection during dental care, which could put immunocompromised individuals at risk of cross-infection. Th...
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MAT2A inhibition suppresses inflammation in Porphyromonas gingivalis-infected human gingival fibroblasts J. Oral Microbiol. (IF 4.5) Pub Date : 2023-12-15 Lishan Jiang, Jingwen Li, Kun Ji, Lang Lei, Houxuan Li
Methionine adenosyl transferase II alpha (MAT2A) is the key enzyme to transform methionine into S-adenosylmethionine (SAM), the main methylgroup donor involved in the methylation. The purpose of ou...
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Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-12-12 Anna Golda, Anna Gasiorek, Ewelina Dobosz, Zuzanna Oruba, Richard J. Lamont, Jan Potempa, Joanna Koziel
Three-dimensional (3D) tissue models bridge the gap between conventional two-dimensional cell cultures and animal models. The aim of this study was to develop an organotypic 3D gingival (OTG) model...
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Oxidative stress response: a critical factor affecting the ecological competitiveness of Streptococcus mutans J. Oral Microbiol. (IF 4.5) Pub Date : 2023-12-12 Xingqun Cheng, Xin Xu, Xuedong Zhou, Jia Ning
Oral microecological balance is closely associated with the development of dental caries. Oxidative stress is one of the important factors regulating the composition and structure of the oral micro...
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Deficiency of dltD contributes to increased autolysis in Streptococcus mutans J. Oral Microbiol. (IF 4.5) Pub Date : 2023-12-10 Jingyun Du, Shan Huang, Yijun Li, Minjing Wu, Shuai Chen, Ling Zhan, Xiaojing Huang
Streptococcus mutans (S. mutans) is one of the major etiological agents of dental caries. Interfering with the expression of genes associated with cariogenicity of S. mutans is an effective ecologi...
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Ultrasmall epibiont Nanosynbacter lyticus strain TM7x and host bacteria transcriptional activity after initial host parasitism J. Oral Microbiol. (IF 4.5) Pub Date : 2023-11-30 Erik L Hendrickson, Batbileg Bor, Kristopher A. Kerns, Lujia Cen, Wenyuan Shi, Xuesong He, Jeffrey S McLean
Oral Saccharibacteria Nanosynbacter lyticus strain TM7× lives as an ultrasmall epibiont on the surface of its host, Schaalia odontolytica strain XH001. Establishing this interaction is a poorly und...
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Sub-lethal concentrations of chlorhexidine inhibit Candida albicans growth by disrupting ROS and metal ion homeostasis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-11-09 Qian Jiang, Yuchen Deng, Shuaihu Li, Deqin Yang, Li Tao
Candida albicans is a normal resident of the human oral cavity. It is also the most common fungal pathogen, causing various oral diseases, particularly in immunocompromised individuals. Chlorhexidi...
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Multiomics analysis reveals the genetic and metabolic characteristics associated with the low prevalence of dental caries J. Oral Microbiol. (IF 4.5) Pub Date : 2023-11-02 Jinshen Liu, Si-Ying Ye, Xin-Dong Xu, Qiulin Liu, Fei Ma, Xueting Yu, Yu-Hong Luo, Ling-Ling Chen, Xiaojuan Zeng
Despite poor oral hygiene, the Baiku Yao (BKY) ethnic group in China presents a low prevalence of dental caries, which may be related to genetic susceptibility. Due to strict intra-ethnic marriage ...
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Oral microbiota study of the patients after hospitalisation for COVID-19, considering selected dental indices and antibiotic therapy using the next generation sequencing method (NGS) J. Oral Microbiol. (IF 4.5) Pub Date : 2023-10-11 Barbara Brzychczy- Sroka, Katarzyna Talaga-Ćwiertnia, Agnieszka Sroka-Oleksiak, Artur Gurgul, Elżbieta Zarzecka-Francica, Wojciech Ostrowski, Janusz Kąkol, Joanna Zarzecka, Monika Brzychczy-Włoch
Poor oral hygiene and the increased incidence and severity of periodontitis may exacerbate SARS-CoV-2 infection. The aim was to evaluate the oral microbiota of 60 participants divided into groups: ...
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Alterations of oral microbiota are associated with the development and severity of acute pancreatitis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-10-05 Yiting Liu, Hang Liu, Yuping Rong, Qiao Shi, Qiang Yang, Hanjun Li, Zhengle Zhang, Jing Tao
Acute pancreatitis (AP) is a common abdomen clinical emergency. Most APs have mild clinical symptoms and a good prognosis. However, about 20% of patients develop severe acute pancreatitis (SAP), in...
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Oral microbiome and ischemic stroke risk among elderly Chinese women J. Oral Microbiol. (IF 4.5) Pub Date : 2023-10-08 Cong Wang, Yaohua Yang, Qiuyin Cai, Yutang Gao, Hui Cai, Jie Wu, Wei Zheng, Jirong Long, Xiao-Ou Shu
Stroke, a leading cause of disability worldwide, has been associated with periodontitis. However, whether stroke risk is related to oral microbiota remains unknown. This study aims to evaluate the ...
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Smoking, tooth loss and oral hygiene practices have significant and site-specific impacts on the microbiome of oral mucosal surfaces: a cross-sectional study J. Oral Microbiol. (IF 4.5) Pub Date : 2023-10-02 Sheila Galvin, Sviatlana Anishchuk, Claire M. Healy, Gary P. Moran
We investigated bacterial colonisation patterns of healthy mucosa (buccal, tongue, palate and floor of mouth) in a cohort of adults in order to determine how smoking, tooth loss, plaque levels and ...
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A tightly controlled gene induction system that contributes to the study of lethal gene function in Streptococcus mutans J. Oral Microbiol. (IF 4.5) Pub Date : 2023-09-07 Yongliang Li, Guanwen Li, Xuliang Deng
ABSTRACT Effective control of gene expression is crucial for understanding gene function in both eukaryotic and prokaryotic cells. While several inducible gene expression systems have been reported in Streptococcus mutans, a conditional pathogen that causes dental caries, the significant non-inducible basal expression in these systems seriously limits their utility, especially when studying lethal
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Parkinson’s disease alters the composition of subgingival microbiome J. Oral Microbiol. (IF 4.5) Pub Date : 2023-08-28 Ekin Yay, Melis Yilmaz, Hilal Toygar, Nur Balci, Carla Alvarez Rivas, Basak Bolluk Kilic, Ali Zirh, Bruce Paster, Alpdogan Kantarci
ABSTRACT Aim The current study aimed to test the hypothesis that Parkinson’s disease exacerbates periodontitis by altering its microbiome. Materials and Methods Clinical periodontal parameters were recorded. Subgingival samples from healthy controls, periodontitis patients (PD), and Parkinson’s patients with periodontitis (PA+PD) were analyzed using the checkerboard DNA-DNA hybridization technique
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Screening of heat-killed lactic acid bacteria based on inhibitory activity against oral bacteria and effects of oral administration of heat-killed Ligilactobacillus salivarius CP3365 on periodontal health in healthy participants: a double-blinded, randomized, placebo-controlled trial J. Oral Microbiol. (IF 4.5) Pub Date : 2023-08-28 Shinji Sakata, Yukiko Sakamaki, Masahiro Yuki, Tsutomu Sugaya, Tatsuhiko Hirota
ABSTRACT Objectives The aims of this study were to select heat-killed lactic acid bacteria (HKL) with antibiotic activity and investigate the efficacy of this bacteria in maintaining periodontal parameters in healthy participants. Materials and methods An in vitro evaluation was conducted to assess the inhibitory efficacy of lactic acid bacteria against Porphyromonas gingivalis and Fusobacterium nucleatum
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Human oral mucosa and oral microbiome interactions following supragingival plaque reconstitution in healthy volunteers: a diet-controlled balanced design proof-of-concept model to investigate oral pathologies J. Oral Microbiol. (IF 4.5) Pub Date : 2023-08-22 Jean-Luc C. Mougeot, Micaela F. Beckman, Darla S. Morton, Jenene Noll, Nury M. Steuerwald, Michael T. Brennan, Farah Bahrani Mougeot
ABSTRACT Changes in the oral microbiome may contribute to oral pathologies, especially in patients undergoing cancer therapy. Interactions between oral microbiome and oral mucosa may exacerbate inflammation. We determined whether probiotic-controlled plaque formation could impact proximal oral mucosa gene expression profiles in healthy volunteers. A 3-weeks balanced sample collection design from healthy
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Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens J. Oral Microbiol. (IF 4.5) Pub Date : 2023-07-31 Wenya Xue, Manon F. Pritchard, Saira Khan, Lydia C. Powell, Joana Stokniene, Jingxiang Wu, Nicholas Claydon, Paul Reddell, David W. Thomas, Katja E. Hill
ABSTRACT Background Peri-implantitis has become an inexorable clinical challenge in implantology. Topical immunomodulatory epoxy-tiglianes (EBCs), derived from the Queensland blushwood tree, which induce remodeling and resolve dermal infection via induction of the inflammasome and biofilm disruption, may offer a novel therapeutic approach. Design In vitro antimicrobial activity of EBC structures (EBC-46
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Trans-cinnamaldehyde loaded chitosan based nanocapsules display antibacterial and antibiofilm effects against cavity-causing Streptococcus mutans. J. Oral Microbiol. (IF 4.5) Pub Date : 2023-08-02 Ran Mu,Hanyi Zhang,Zhiyuan Zhang,Xinyue Li,Jiaxuan Ji,Xinyue Wang,Yu Gu,Xiaofei Qin
Background Dental caries is a multifactorial disease, and the bacteria such as Streptococcus mutans (S. mutans) is one of the risk factors. The poor effect of existing anti-bacterial is mainly related to drug resistance, the short time of drug action, and biofilm formation. Methods To address this concern, we report here on the cinnamaldehyde (CA) loaded chitosan (CS) nanocapsules (CA@CS NC) sustained
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CKIP-1 mediates P. gingivalis-suppressed osteogenic/cementogenic differentiation of periodontal ligament cells partially via p38 signaling pathway J. Oral Microbiol. (IF 4.5) Pub Date : 2023-07-20 Xin Huang, Junhong Xiao, Huiyi Wang, Yan Peng, Heyu Liu, Li Ma, Xiaoxuan Wang, Zhengguo Cao
ABSTRACT Objectives Casein kinase 2 interacting protein-1 (CKIP-1) is a versatile player involved in various biological processes. However, whether CKIP-1 mediates the osteogenic/cementogenic differentiation of periodontal ligament cells (PDLCs) under Porphyromonas gingivalis (Pg) stimulation remains unknown. Material and Methods The effect of Pg on PDLC differentiation was first verified. CKIP-1 expression
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Tongue coating microbiome composition reflects disease severity in patients with COVID-19 in Nanjing, China J. Oral Microbiol. (IF 4.5) Pub Date : 2023-07-19 Zongdan Jiang, Lu Yang, Xuetian Qian, Kunhan Su, Yuzhen Huang, Yi Qu, Zhenyu Zhang, Wanli Liu
ABSTRACT Our purpose is to investigate the relationship between the microbiota of patients' tongue coating microbiota and the severity of COVID-19, and to identify the severity of COVID-19 patients' condition as early as possible. The participants were categorized into three groups: healthy controls (Con group) consisting of 37 individuals, patients with mild to moderate symptoms (M group) comprising
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Inhibition of biofilm formation and virulence factors of cariogenic oral pathogen Streptococcus mutans by natural flavonoid phloretin J. Oral Microbiol. (IF 4.5) Pub Date : 2023-07-03 Lucille Rudin, Michael M. Bornstein, Viktoriya Shyp
ABSTRACT Objectives To evaluate the effect and mechanism of action of the flavonoid phloretin on the growth and sucrose-dependent biofilm formation of Streptococcus mutans. Methods Minimum inhibitory concentration, viability, and biofilm susceptibility assays were conducted to assess antimicrobial and antibiofilm effect of phloretin. Biofilm composition and structure were analysed with scanning electron
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Exploration of the interplay between spatially distinct microbial habitats through comparative analysis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-06-27 Hyunji Kim, Jin-Sil Hong, Pil-Young Yun, Kyung-Gyun Hwang, Keun-Suh Kim, Hyo-Jung Lee, Kyoung Un Park
ABSTRACT Objectives The oral microbiome is closely associated with systemic diseases, indicating the presence of bacteremia and inflammatory mediators in the systemic circulation. Our research aims to investigate the relationship between the oral microbiome and other microbial habitats. Methods We analyzed 180 specimens from 36 patients, including saliva, buccal swab, plaque, stool, and blood samples
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Site-specialization of human oral Gemella species J. Oral Microbiol. (IF 4.5) Pub Date : 2023-06-22 Julian Torres-Morales, Jessica L. Mark Welch, Floyd E. Dewhirst, Gary G. Borisy
ABSTRACT Gemella species are core members of the human oral microbiome in healthy subjects and are regarded as commensals, although they can cause opportunistic infections. Our objective was to evaluate the site-specialization of Gemella species among various habitats within the mouth by combining pangenomics and metagenomics. With pangenomics, we identified genome relationships and categorized genes
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Regulatory mechanisms of exopolysaccharide synthesis and biofilm formation in Streptococcus mutans J. Oral Microbiol. (IF 4.5) Pub Date : 2023-06-18 Ting Zheng, Meiling Jing, Tao Gong, Jiangchuan Yan, Xiaowan Wang, Mai Xu, Xuedong Zhou, Jumei Zeng, Yuqing Li
ABSTRACT Background Dental caries is a chronic, multifactorial and biofilm-mediated oral bacterial infection affecting almost every age group and every geographical region. Streptococcus mutans is considered an important pathogen responsible for the initiation and development of dental caries. It produces exopolysaccharides in situ to promote the colonization of cariogenic bacteria and coordinate dental
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First water safety plan approach applied to a Dental Clinic complex: identification of new risk factors associated with Legionella and P. aeruginosa contamination, using a novel sampling, maintenance and management program J. Oral Microbiol. (IF 4.5) Pub Date : 2023-06-16 Federica Marino, Marta Mazzotta, Maria Rosaria Pascale, Carlo Derelitto, Luna Girolamini, Sandra Cristino
ABSTRACT Dental unit waterlines (DUWLs) represent a complex environment able to promote microbial contamination, due to functional, mechanical and practical risk factors. According to a water safety plan approach, the main goal is to preserve the health of dentists, dental staff and patients. The aim of this study is to develop a DUWLs water safety plan that is able to support correct and effective
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Fusobacterium nucleatum dissemination by neutrophils J. Oral Microbiol. (IF 4.5) Pub Date : 2023-06-04 Felix Ellett, Nazli I. Kacamak, Carla Alvarez, Eduardo H.S. Oliveira, Hatice Hasturk, Bruce J. Paster, Alpdogan Kantarci, Daniel Irimia
ABSTRACT Recent studies uncovered that Fusobacterium nucleatum (Fn), a common, opportunistic bacterium in the oral cavity, is associated with a growing number of systemic diseases, ranging from colon cancer to Alzheimer’s disease. However, the pathological mechanisms responsible for this association are still poorly understood. Here, we leverage recent technological advances to study the interactions
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Newly identified pathogens in periodontitis: evidence from an association and an elimination study J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-27 Eduardo Lobão Veras, Nídia Castro dos Santos, João Gabriel S. Souza, Luciene C. Figueiredo, Belen Retamal-Valdes, Valentim A. R. Barão, Jamil Shibli, Martinna Bertolini, Marcelo Faveri, Flavia Teles, Poliana Duarte, Magda Feres
ABSTRACT We assessed the level of evidence for the presence of new periodontal pathogens by (i) comparing the occurrence of non-classical periodontal taxa between healthy vs. periodontitis patients (Association study); (ii) assessing the modifications in the prevalence and levels of these species after treatments (Elimination study). In the Association study, we compared the prevalence and levels of
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Structural and antigenic characterization of a novel genotype of Mfa1 fimbriae in Porphyromonas gingivalis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-21 Miyuna Fujimoto, Yoshikazu Naiki, Kotaro Sakae, Tomohiko Iwase, Naoyoshi Miwa, Keiji Nagano, Hiroyuki Nawa, Yoshiaki Hasegawa
ABSTRACT Background Mfa1 fimbriae of the periodontal pathogen Porphyromonas gingivalis are responsible for biofilm formation and comprise five proteins: Mfa1–5. Two major genotypes, mfa170 and mfa153, encode major fimbrillin. The mfa170 genotype is further divided into the mfa170A and mfa170B subtypes. The properties of the novel mfa170B remain unclear. Methods Fimbriae were purified from P. gingivalis
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SARS-CoV-2 RT-qPCR Ct values in saliva and nasopharyngeal swab samples for disease severity prediction J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-17 Kristina Snipaitiene, Birute Zablockiene, Rasa Sabaliauskaite, Kristina Zukauskaite, Elzbieta Matulyte, Tautvile Smalinskaite, Mindaugas Paulauskas, Rolandas Zablockis, Mantvydas Lopeta, Julius Gagilas, Alina Puriene, Ligita Jancoriene, Sonata Jarmalaite
ABSTRACT Background Comparison of clinical value of RT-qPCR-based SARS-CoV-2 tests performed on saliva samples (SSs) and nasopharyngeal swab samples (NPSs) for prediction of the COVID-19 disease severity. Methods Three paired SSs and NPSs collected every 3 days from 100 hospitalised COVID-19 patients during 2020 Jul-2021 Jan were tested by RT-qPCR for the original SARS-CoV-2 virus and compared to 150
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Hemophore-like proteins produced by periodontopathogens are recognized by the host immune system and react differentially with IgG antibodies J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-17 Michał Śmiga, Klaudia Siemińska, Soraya C. Trindade, Isaac S. Gomes-Filho, Ellen K. Nobre dos Santos, Teresa Olczak
ABSTRACT Aims Hemophore-like proteins sequester heme from host hemoproteins. We aimed to determine whether the host immune system can recognize not only Porphyromonas gingivalis HmuY but also its homologs expressed by other periodontopathogens, and how periodontitis influences the production of respective antibodies. Methods The reactivity of total bacterial antigens and purified proteins with serum
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The interplay between oral microbiota, gut microbiota and systematic diseases J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-15 Xiujun Tan, Yizhong Wang, Ting Gong
ABSTRACT Over the past two decades, the importance of microbiota in health and disease has become evident. The human gut microbiota and oral microbiota are the largest and second-largest microbiome in the human body, respectively, and they are physically connected as the oral cavity is the beginning of the digestive system. Emerging and exciting evidence has shown complex and important connections
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Rapid specific detection of oral bacteria using Cas13-based SHERLOCK J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-11 Jett Liu, Camden Carmichael, Hatice Hasturk, Wenyuan Shi, Batbileg Bor
ABSTRACT Decades of ongoing research has established that oral microbial communities play a role in oral diseases such as periodontitis and caries. Yet the detection of oral bacteria and the profiling of oral polymicrobial communities currently rely on methods that are costly, slow, and technically complex, such as qPCR or next-generation sequencing. For the widescale screening of oral microorganisms
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Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogen Enterococcus faecalis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-05-11 Ana Parga, Daniel Manoil, Malin Brundin, Ana Otero, Georgios N. Belibasakis
ABSTRACT Acyl-homoserine lactones (AHLs) are typical quorum-sensing molecules of gram-negative bacteria. Recent evidence suggests that AHLs may also affect gram-positives, although knowledge of these interactions remains scarce. Here, we assessed the effect of AHLs on biofilm formation and transcriptional regulations in the gram-positive Enterococcus faecalis. Five E. faecalis strains were investigated
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Exopolysaccharides metabolism and cariogenesis of Streptococcus mutans biofilm regulated by antisense vicK RNA J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-28 Yuting Sun, Hong Chen, Mengmeng Xu, Liwen He, Hongchen Mao, Shiyao Yang, Xin Qiao, Deqin Yang
ABSTRACT Background Streptococcus mutans (S. mutans) is a pivotal cariogenic pathogen contributing to its multiple virulence factors, one of which is synthesizing exopolysaccharides (EPS). VicK, a sensor histidine kinase, plays a major role in regulating genes associated with EPS synthesis and adhesion. Here we first identified an antisense vicK RNA (ASvicK) bound with vicK into double-stranded RNA
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Mechanisms of Porphyromonas gingivalis to translocate over the oral mucosa and other tissue barriers J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-26 Caroline A. de Jongh, Teun J. de Vries, Floris J. Bikker, Susan Gibbs, Bastiaan P. Krom
ABSTRACT Introduction The oral pathogen Porphyromonas gingivalis is not only associated with periodontitis but also with systemic diseases elsewhere in the body. The mechanisms by which P. gingivalis travels from the oral cavity to other organs in the body are largely unknown. This review describes the four putative mechanisms supported by experimental evidence, which enable translocation of P. gingivalis
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In vivo effect of mouthwashes on viable viral load of SARS-CoV-2 in saliva: a pilot study J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-11 Alvaro Sánchez Barrueco, María Victoria Mateos-Moreno, José Miguel Villacampa Aubá, Alfonso Campos González, Abel Bogoya Castaño, Raúl Rubio Yanguas, Asier Blanco Goñi, Javier Zapardiel Ferrero, Carlos Cenjor Español, Verónica Ausina Márquez, Sandra García-Esteban, Alejandro Artacho, F. Xavier López Labrador, Alex Mira, María D. Ferrer
ABSTRACT Current data on the efficacy of antiseptic mouthwashes to reduce viral load are contradictory. Firstly, in vitro data indicate very strong virucidal effects that are not replicated in clinical studies. Secondly, most clinical studies identify a limited effect, do not include a control/placebo group, or do not evaluate viral viability in an infection model. In the current manuscript, we perform
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Profiles of oral microbiome associated with nasogastric tube feeding J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-11 Ding-Han Wang, Fa-Tzu Tsai, Hsi-Feng Tu, Cheng-Chieh Yang, Ming-Lun Hsu, Lin-Jack Huang, Chiu-Tzu Lin, Wun-Eng Hsu, Yu-Cheng Lin
ABSTRACT Background Dysbiosis of oral microbiome causes chronic diseases including dental caries and periodontitis, which frequently affect older patient populations. Severely disabled individuals with impaired swallowing functions may require nutritional supply via nasogastric (NG) tubes, further impacting their oral condition and possibly microbial composition. However, little is known about the
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Dental caries and their microbiomes in children: what do we do now? J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-10 Apoena Aguiar Ribeiro, Bruce J. Paster
ABSTRACT The oral cavity is an unique ecosystem formed by different structures, tissues, and a complex microbial community formed by hundreds of different species of bacteria, fungi, viruses, phages, and the candidate phyla radiation (CPR) group, all living in symbiosis with healthy individuals. In an opposite state, dental caries is a biofilm-mediated dysbiosis that involves changes in the core microbiome
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Interaction between microorganisms and dental material surfaces: general concepts and research progress J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-05 Yan Tu, Huaying Ren, Yiwen He, Jiaqi Ying, Yadong Chen
ABSTRACT Bacterial adhesion to dental materials’ surfaces is the initial cause of dental materials-related infections. Therefore, inhibiting bacterial adhesion is a critical step in preventing and controlling these infections. To this end, it is important to know how the properties of dental materials affect the interactions between microorganisms and material surfaces to produce materials without
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Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis J. Oral Microbiol. (IF 4.5) Pub Date : 2023-04-02 Ali A. Abdulkareem, Firas B. Al-Taweel, Ali J.B. Al-Sharqi, Sarhang S. Gul, Aram Sha, Iain L.C. Chapple
ABSTRACT The primary etiological agent for the initiation and progression of periodontal disease is the dental plaque biofilm which is an organized aggregation of microorganisms residing within a complex intercellular matrix. The non-specific plaque hypothesis was the first attempt to explain the role of the dental biofilm in the pathogenesis of periodontal diseases. However, the introduction of sophisticated
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Exploring the biomedical potential of a novel modified glass ionomer cement against the pandrug-resistant oral pathogen Candida albicans SYN-01 J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-30 Nessma A. El Zawawy, Samy El-Safty, El-Refaie Kenawy, Sara Ibrahim Salem, Sameh S. Ali, Yehia A.-G. Mahmoud
ABSTRACT Dental caries is an infectious disease that is a major concern for dentists. Streptococci and Lactobacilli were long thought to be the primary etiology responsible for caries. Candida albicans with acidogenic and aciduric characteristics has recently been implicated in the onset and progression of cariogenic lesions. Moreover, due to the increased resistance to common antimicrobials, the discovery
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Association of nine pathobionts with periodontitis in four South American and European countries J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-19 Gerard Àlvarez, Alexandre Arredondo, Sergio Isabal, Wim Teughels, Isabelle Laleman, María José Contreras, Lorena Isbej, Enrique Huapaya, Gerardo Mendoza, Carolina Mor, José Nart, Vanessa Blanc, Rubén León
ABSTRACT Aim Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries. Methods Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis patients in Belgium, Chile, Peru and Spain. The prevalence and load of Eubacterium brachy, Filifactor alocis
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Short-term sugar stress induces compositional changes and loss of diversity of the supragingival microbiota J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-21 Christine Lundtorp Olsen, Merete Markvart, Vincent Frederik Dahl Vendius, Christian Damgaard, Daniel Belstrøm
ABSTRACT Frequent intake of free sugars is a major risk factor for dental caries, but the immediate influence of sugar intake on the supragingival microbiota remains unknown. We aim to characterize the effect of 14 days of sugar rinsing on the supragingival microbiota. Forty orally and systemically healthy participants rinsed their mouth with a 10% sucrose solution, 6–8 times a day, for 14 days, followed
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Mode of killing determines the necrotrophic response of oral bacteria J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-06 Naiera Zayed, Joana Figueiredo, Wannes Van Holm, Nico Boon, Kristel Bernaerts, Wim Teughels
ABSTRACT Background Bacteria respond to changes in their environment, such as nutrient depletion and antimicrobials exposure. Antimicrobials result not only in bacterial death, but also have a hand in determining species abundances and ecology of the oral biofilms. Proximity of dead bacterial cells to living ones is an important environmental change or stress factor. Dead bacteria represent high concentrations
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Understanding the bacterial compositional network associations between oral and gut microbiome within healthy Koreans J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-03 Jinuk Jeong, Kung Ahn, Seyoung Mun, Kyeongeui Yun, Yeon-Tae Kim, Won Jung, Kyung Eun Lee, Moon-Young Kim, Yongju Ahn, Kyudong Han
ABSTRACT Oral microbial ecosystem could influence intestinal diseases, but there have been insufficient studies demonstrating the association of microbial composition between the oral cavity and the intestinal system. Thus, we aimed to investigate the compositional network within the oral microbiome related to gut enterotype from saliva and stool samples collected from 112 healthy Korean subjects.
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Analysis of oral microbiota in non-vital teeth and clinically intact external surface from patients with severe periodontitis using Nanopore sequencing: a case study J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-03 Alessio Buonavoglia, Francesco Pellegrini, Gianvito Lanave, Georgia Diakoudi, Maria Stella Lucente, Fausto Zamparini, Michele Camero, Maria Giovanna Gandolfi, Vito Martella, Carlo Prati
ABSTRACT Periodontal diseases include a wide range of pathological conditions, damaging the supporting structures of the teeth. Origin and propagation of periodontal disease is believed to be caused by dysbiosis of the commensal oral microbiota. The aim of this study was to evaluate the presence of bacteria in the pulp cavity of teeth with severe periodontal disease with clinically intact external
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A mouthwash formulated with o-cymen-5-ol and zinc chloride specifically targets potential pathogens without impairing the native oral microbiome in healthy individuals J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-03 Javier Pascual, Javier Mira Otal, Daniel Torrent-Silla, Manuel Porcar, Cristina Vilanova, Fernando Vivancos Cuadras
ABSTRACT Background Many antimicrobial compounds in mouthwashes can have a negative impact on the oral microbiome. O-cymen-5-ol, a compound derived from a phytochemical, has a targeted mode of action and is being used as an alternative. However, its effect on the native oral microbiome is unknown. Aim To assess the effect of a mouthwash formulated with o-cymen-5-ol and zinc chloride on the oral microbiome
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Role of Enterococcus faecalis in refractory apical periodontitis: from pathogenicity to host cell response J. Oral Microbiol. (IF 4.5) Pub Date : 2023-03-01 Zilong Deng, Binbin Lin, Fan Liu, Wanghong Zhao
ABSTRACT Background: Refractory apical periodontitis (RAP) is an oral infectious disease characterised by persistent inflammation, progressive alveolar bone destruction, and delayed bone healing. RAP has received increasing attention, because it cannot be cured after repeated root canal therapies. The aetiology of RAP is related to the complex interplay between the pathogen and its host. However, the
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Changes in the oral and nasal microbiota in pediatric obstructive sleep apnea J. Oral Microbiol. (IF 4.5) Pub Date : 2023-02-28 Xiaoman Zhang, Xinyi Li, Huajun Xu, Zhihui Fu, Fan Wang, Weijun Huang, Kejia Wu, Chenyang Li, Yupu Liu, Jianyin Zou, Huaming Zhu, Hongliang Yi, Su Kaiming, Meizhen Gu, Jian Guan, Shankai Yin
ABSTRACT Background Several clinical studies have demonstrated that pediatric obstructive sleep apnea (OSA) is associated with dysbiosis of airway mucosal microbiota. However, how oral and nasal microbial diversity, composition, and structure are altered in pediatric OSA has not been systemically explored. Methods 30 polysomnography-confirmed OSA patients with adenoid hypertrophy, and 30 controls who
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Saliva microbiome alterations in dental fluorosis population J. Oral Microbiol. (IF 4.5) Pub Date : 2023-02-20 Shanshan Liu, Qiangsheng Song, Chenchen Zhang, Mengwan Li, Zhenzhen Li, Yudong Liu, Li Xu, Xiaofei Xie, Lili Zhao, Rongxiu Zhang, Qinglong Wang, Guojin Zeng, Yifan Zhang, Kai Zhang
ABSTRACT Background We aimed to explore saliva microbiome alterations in dental fluorosis population. Methods The prevalence of dental fluorosis was examined in 957 college students. Dean’s fluorosis index was used to evaluate the dental fluorosis status. Changes in the composition of the salivary microbiome were assessed in a subset of these patients (100 healthy controls, 100 dental fluorosis patients)