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IL-2-mediated hepatotoxicity: knowledge gap identification based on the irAOP concept J. Immunotoxicol. (IF 3.3) Pub Date : 2024-04-05 Luise A. Roser, Christina Sakellariou, Malin Lindstedt, Vanessa Neuhaus, Susann Dehmel, Charline Sommer, Martin Raasch, Thierry Flandre, Sigrid Roesener, Philip Hewitt, Michael J. Parnham, Katherina Sewald, Susanne Schiffmann
Drug-induced hepatotoxicity constitutes a major reason for non-approval and post-marketing withdrawal of pharmaceuticals. In many cases, preclinical models lack predictive capacity for hepatic dama...
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Lung-delivered IL-10 therapy elicits beneficial effects via immune modulation in organic dust exposure-induced lung inflammation J. Immunotoxicol. (IF 3.3) Pub Date : 2024-04-02 Aaron D. Schwab, Todd A. Wyatt, Amy J. Nelson, Angela Gleason, Rohit Gaurav, Debra J. Romberger, Jill A. Poole
Efficacious therapeutic options capable of resolving inflammatory lung disease associated with environmental and occupational exposures are lacking. This study sought to determine the preclinical t...
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Fine tuning of the innate and adaptive immune responses by Interleukin-2 J. Immunotoxicol. (IF 3.3) Pub Date : 2024-03-25 Christina Sakellariou, Luise A. Roser, Susanne Schiffmann, Malin Lindstedt
Novel immunotherapies for cancer and other diseases aim to trigger the immune system to produce durable responses, while overcoming the immunosuppression that may contribute to disease severity, an...
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Identification and semi-quantification of protein allergens in complex mixtures using proteomic and AllerCatPro 2.0 bioinformatic analyses: a proof-of-concept investigation J. Immunotoxicol. (IF 3.3) Pub Date : 2024-01-31 Nora L. Krutz, Ian Kimber, Jason Winget, Minh N. Nguyen, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Catherine Mahony, G. Frank Gerberick
The demand for botanicals and natural substances in consumer products has increased in recent years. These substances usually contain proteins and these, in turn, can pose a risk for immunoglobulin...
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Investigation into changes in inflammatory and immune cell markers in pre-diabetic patients from Durban, South Africa J. Immunotoxicol. (IF 3.3) Pub Date : 2023-12-15 Nomusa Christina Mzimela, Aubrey Mbulelo Sosibo, Phikelelani Siphosethu Ngubane, Andile Khathi
The prevalence of pre-diabetes is increasing in rapidly urbanizing cities, especially in individuals aged 25 − 45 years old. Studies also indicate that this condition is associated with aberrant im...
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Vitamin D/Vitamin D receptor mitigates cisplatin-induced acute kidney injury by down-regulating C5aR J. Immunotoxicol. (IF 3.3) Pub Date : 2023-09-05 Zhouyu Wen, Can Sun, Yan Lou, Juan Kong
Abstract Cisplatin (DDP) is a potent chemotherapeutic; however, it can also cause acute kidney injury (AKI). Because of the complexity of the toxicity it induces, few effective methods exist for ameliorating any form of DDP-induced AKI. Recent research has suggested that the complement system is a potential molecular target for such amelioration. In the study here, in vivo (male ICR mice) and in vitro
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Yin Yang 1 impacts upon preeclampsia by regulating Treg/TH17 cells and PI3K/AKT pathway J. Immunotoxicol. (IF 3.3) Pub Date : 2023-07-19 Haowen Li, Ling Yu, Yiling Ding, Yanting Nie, Mengyuan Yang
Abstract Preeclampsia (PE) is a common obstetric syndrome with an unclear etiology and pathogenesis. The study here aimed to investigate the role of Yin Yang 1 (YY1) in PE, and to reveal any YY1-regulated mechanisms in PE. Peripheral blood, placenta, and endometrial tissues of PE patients, healthy volunteers, and patients who had undergone an elective Cesarean section and had a scarred uterus (control
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Endoplasmic reticulum stress mediates environmental particle-induced inflammatory response in bronchial epithelium J. Immunotoxicol. (IF 3.3) Pub Date : 2023-07-07 Li Pu, Fen Yi, Wen-jing Yu, Ya-jing Li, You-hui Tu, Ai-hui Xu, Yong Wang
Abstract While the detailed mechanisms for how particulate matter (PM) causes adverse health effects in the lungs remain largely unknown, endoplasmic reticulum (ER) stress has been implicated in PM-induced lung injury. The present study was undertaken to examine how/if ER stress might regulate PM-induced inflammation, and to begin to define potential underlying molecular mechanisms. Here, ER stress
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Hsa_circ_0044235 and hsa_circ_0001947 as novel biomarkers in plasma of patients with new-onset systemic lupus erythematosus J. Immunotoxicol. (IF 3.3) Pub Date : 2023-04-05 Qing Luo, Yutao Ye, Lu Zhang, Yujie Gao, Jiayue Rao, Yang Guo, Qingshui Huang, Zikun Huang, Junming Li
Abstract Circular RNA (circRNA) are novel types of non-coding RNA that may be used as non-invasive noninvasive biomarkers in clinical plasma samples. However, the role of circRNA in plasma samples from patients with new-onset systemic lupus erythematosus (SLE) has not been extensively investigated. In the present study, reverse transcription-quantitative PCR was used to screen differentially-expressed
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Current approaches to evaluate the function of cytotoxic T-cells in non-human primates J. Immunotoxicol. (IF 3.3) Pub Date : 2023-02-14 Cris Kamperschroer, Brendon Frank, Caroline Genell, Hervé Lebrec, Shermaine Mitchell-Ryan, Brigitte Molinier, Courtni Newsome, Marie-Soleil Piche, Daniel Weinstock, Mark Collinge, Wendy Freebern, Daniel Rubio
Abstract Cytotoxic T-lymphocytes (CTL) are a subset of T-cells that play a critical role in protecting against intracellular infections and cancer, and have the ability to identify and kill infected or transformed cells expressing non-self peptides associated with major histocompatibility (MHC) Class I molecules. Conversely, aberrant CTL activity can contribute to immune-related pathology under conditions
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Legacy and emerging per- and polyfluoroalkyl substances suppress the neutrophil respiratory burst J. Immunotoxicol. (IF 3.3) Pub Date : 2023-02-14 Drake W. Phelps, Anika I. Palekar, Haleigh E. Conley, Giuliano Ferrero, Jacob H. Driggers, Keith E. Linder, Seth W. Kullman, David M. Reif, M. Katie Sheats, Jamie C. DeWitt, Jeffrey A. Yoder
Abstract Per- and polyfluoroalkyl substances (PFASs) are used in a multitude of processes and products, including nonstick coatings, food wrappers, and fire-fighting foams. These chemicals are environmentally-persistent, ubiquitous, and can be detected in the serum of 98% of Americans. Despite evidence that PFASs alter adaptive immunity, few studies have investigated their effects on innate immunity
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Silver nanoparticles induce a non-immunogenic tumor cell death J. Immunotoxicol. (IF 3.3) Pub Date : 2023-02-11 Maritza Roxana Garcia Garcia, Noelia Casares, Luz Andrea Martinez Perez, Efren Juarez Curiel, Andres Alberto de Jesus Hernandez, Nina Bogdanchikova, Diana Garibo, Ana G. Rodriguez-Hernandez, Alexey Pestryakov, Sandra Castro Gamboa, Luis Felipe Arias Ruiz, Olivia Torres Bugarin, Pedro Berraondo
Abstract Immunogenic cell death (ICD) is a form of cell death characterized by the release of danger signals required to trigger an adaptive immune response against tumor-associated antigens. Silver nanoparticles (AgNP) display anti-proliferative and cytotoxic effects in tumor cells, but it has not been previously studied whether AgNP act as an ICD inductor. The present study evaluated the in vitro
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Effect of epithelial-specific MyD88 signaling pathway on airway inflammatory response to organic dust exposure J. Immunotoxicol. (IF 3.3) Pub Date : 2022-12-20 Amber N. Johnson, John Dickinson, Amy Nelson, Rohit Gaurav, Katrina Kudrna, Scott E. Evans, Katherine Janike, Todd A. Wyatt, Jill A. Poole
Abstract The Toll-like receptor (TLR) adaptor protein MyD88 is integral to airway inflammatory response to microbial-enriched organic dust extract (ODE) exposures. ODE-induced airway neutrophil influx and release of pro-inflammatory cytokines was essentially abrogated in global MyD88-deficient mice, yet these mice demonstrate an increase in airway epithelial cell mucin expression. To further elucidate
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Exposure to the anti-microbial chemical triclosan disrupts keratinocyte function and skin integrity in a model of reconstructed human epidermis J. Immunotoxicol. (IF 3.3) Pub Date : 2022-12-16 Rachel Baur, Michael Kashon, Ewa Lukomska, Lisa M. Weatherly, Hillary L. Shane, Stacey E. Anderson
Abstract Triclosan is an anti-microbial chemical incorporated into products that are applied to the skin of healthcare workers. Exposure to triclosan has previously been shown to be associated with allergic disease in humans and impact the immune responses in animal models. Additionally, studies have shown that exposure to triclosan dermally activates the NLRP3 inflammasome and disrupts the skin barrier
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Environmentally weathered polystyrene particles induce phenotypical and functional maturation of human monocyte-derived dendritic cells J. Immunotoxicol. (IF 3.3) Pub Date : 2022-11-24 Annemijne E. T. van den Berg, Maud Plantinga, Dick Vethaak, Kas J. Adriaans, Marianne Bol-Schoenmakers, Juliette Legler, Joost J. Smit, Raymond H. H. Pieters
Abstract Micro- and nanoplastics (MNP) are ubiquitously present in the environment due to their high persistence and bioaccumulative properties. Humans get exposed to MNP via various routes and consequently, they will encounter dendritic cells (DC) which are antigen-presenting cells involved in regulating immune responses. The consequences of DC exposure to MNP are an important, yet understudied, cause
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Dolutegravir potentiates platelet activation by a calcium-dependent, ionophore-like mechanism J. Immunotoxicol. (IF 3.3) Pub Date : 2022-11-17 Morris Madzime, Annette J. Theron, Ronald Anderson, Gregory R. Tintinger, Helen C. Steel, Pieter W. A. Meyer, Jan G. Nel, Charles Feldman, Theresa M. Rossouw
Abstract Dolutegravir is a highly potent HIV integrase strand transfer inhibitor that is recommended for first-line anti-retroviral treatment in all major treatment guidelines. A recent study has shown that people taking this class of anti-retroviral treatment have a substantially higher risk of early-onset cardiovascular disease, a condition shown previously to be associated with increased platelet
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Strategies to evaluate potential effector function of glycan variants: a case study of ordesekimab (AMG 714 or PRV-015) J. Immunotoxicol. (IF 3.3) Pub Date : 2022-09-09 Yu-Ling Wei, Teresa Wegesser, Scott Kuhns, Jonathan Werner, Hervé Lebrec, Xiaoting Wang
Abstract The potential for effector functions of therapeutic antibodies, including antibody-dependent cell-mediated cytotoxicity (ADCC), is a biological activity of interest for characterization, regardless of if ADCC is an intended primary pharmacological effect. The composition of the conserved antibody Fc glycan can vary as a function of post-translational processing which may affect the binding
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ROS-mediated inflammatory response in liver damage via regulating the Nrf2/HO-1/NLRP3 pathway in mice with trichloroethylene hypersensitivity syndrome J. Immunotoxicol. (IF 3.3) Pub Date : 2022-09-07 Feng Wang, Yiting Hong, Wei Jiang, Yican Wang, Muyue Chen, Dandan Zang, Qixing Zhu
Abstract Trichloroethylene hypersensitivity syndrome (THS), mainly caused by occupational exposure to trichloroethylene (TCE), can give rise to serious and fatal hepatic damage. To date, the precise mechanisms of hepatic damage in THS remain unclear. Recent studies showed that reactive oxygen species (ROS) play a core role in cell death and inflammatory response. Therefore, the present study sought
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Bisphenol A, TH17 cells, and allergy: a commentary J. Immunotoxicol. (IF 3.3) Pub Date : 2022-09-07 Ian Kimber, Nicole Woeffen, Kevin Sondenheimer
Abstract There is a continuing interest in whether Bisphenol A (BPA) is able to cause adverse health effects through interaction with elements of the immune system. That interest has been fuelled further by the recent publication of a draft opinion on BPA prepared by the European Food Safety Authority (EFSA) Panel on Food Contact Materials, Enzymes and Processing Aids (CEP). This draft opinion judged
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Mitochondrial disruption in isolated human monocytes: an underlying mechanism for cadmium-induced immunotoxicity J. Immunotoxicol. (IF 3.3) Pub Date : 2022-09-06 Ulfat M. Omar, Ekramy M. Elmorsy, Ayat B. Al-Ghafari
Abstract Cadmium (Cd) is an immunotoxic metal frequently found in the environment. The in vitro study undertaken here evaluated the immunotoxic effects of Cd in isolated human peripheral blood monocytes (hPBM). The results of the studies of exposures to varying doses of Cd (0, 0.1, 1, 10, and 100 µM, as cadmium dichloride [CdCl2]) for 3, 6, 12, 24, 48, and 72 hr showed the test agent was cytotoxic
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Long non-coding RNA PMS2L2 is down-regulated in osteoarthritis and inhibits chondrocyte proliferation by up-regulating miR-34a J. Immunotoxicol. (IF 3.3) Pub Date : 2022-08-05 Fei Yang, Min Zhao, Qinghua Sang, Changhong Yan, Zhenjun Wang
Abstract Long non-coding RNA (lncRNA) PMS2L2 has been reported to participate in endotoxin-induced inflammatory responses. As these types of responses can promote osteoarthritis (OA), it was of interest to ascertain if PMS2L2 may be involved in OA. To explore any potential participation of PMS2L2 in OA, synovial fluid was extracted from both OA patients and healthy controls (n = 62 each) and PMS2L2
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Examining the role of nickel and NiTi nanoparticles promoting inflammation and angiogenesis J. Immunotoxicol. (IF 3.3) Pub Date : 2022-07-28 Anup K. Srivastava, Dustin M. Snapper, Jiwen Zheng, Banu S. Yildrim, Sandeep Srivastava, Steven C. Wood
Abstract Nickel titanium (NiTi, or Nitinol) alloy is used in several biomedical applications, including cardiac, peripheral vascular, and fallopian tube stents. There are significant biocompatibility issues of metallic implants to nickel ions and nano-/micro-sized alloy particles. Our laboratories have recently shown that microscale CoCr wear particles from metal-on-metal hips crosslink with the innate
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Expression and clinical significance of the m6A reader YTHDF2 in peripheral blood mononuclear cells from rheumatoid arthritis patients J. Immunotoxicol. (IF 3.3) Pub Date : 2022-07-01 Fangyi Yao, Chuxin Xu, Yujie Gao, Biqi Fu, Lu Zhang, Yang Guo, Zikun Huang, Xiaozhong Wang, Junming Li, Qing Luo
Abstract As an important m6A reader, the YT521-B homology domain family 2 (YTHDF2) has been shown to regulate mRNA degradation and translation, and to be involved in inflammation. However, little is known about the role of YTHDF2 in the autoimmune-based inflammatory disease rheumatoid arthritis (RA). To begin to ascertain any role for this reader, 74 RA patients and 63 healthy controls (HC) were recruited
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Immunosafety evaluation in Juvenile Göttingen Minipigs J. Immunotoxicol. (IF 3.3) Pub Date : 2022-06-29 Linda Allais, Alicia Perbet, Fabienne Condevaux, Jean-Paul Briffaux, Marc Pallardy
Abstract Although an extrapolation from the clinical experience in adults can often be considered to support the pediatric use for most pharmaceutical compounds, differences in safety profiles between adult and pediatric patients can be observed. The developing immune system may be affected due to exaggerated pharmacological or non-expected effects of a new drug. Toxicology studies in juvenile animals
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A T-cell-dependent antibody response (TDAR) method in BALB/c mice based on a cytometric bead array J. Immunotoxicol. (IF 3.3) Pub Date : 2022-04-27 Wenhua Zhong, Penghuan Chang, Lianfang Gan, Lifan Zhong, Zhaoxin Yang
Abstract Most current methods to assess T-cell-dependent antibody responses (TDAR) are semi-quantitative and based on measures of antibody titer generated against a standard antigen like keyhole limpet hemocyanin (KLH). The precision, sensitivity, and convenience of TDAR assays might be improved by applying rapid, sensitive, specific cytometric bead assays (CBA). In the study here, KLH antigen was
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Proteolytic activity accelerates the TH17/TH22 recall response to an epicutaneous protein allergen-induced TH2 response J. Immunotoxicol. (IF 3.3) Pub Date : 2022-04-04 Akira Ogasawara, Takuo Yuki, Asuka Katagiri, Yi-Ting Lai, Yutaka Takahashi, David Basketter, Hitoshi Sakaguchi
Abstract Epicutaneous exposure to protein allergens, such as papain, house dust mite (HDM), and ovalbumin (OVA), represents an important mode of sensitization for skin diseases including protein contact dermatitis, immunologic contact urticaria, and atopic dermatitis. These diseases are inducible by re-exposure to an allergen at both original skin sensitization and distant skin sites. In this study
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Gallic acid attenuates cerebral ischemia/re-perfusion-induced blood–brain barrier injury by modifying polarization of microglia J. Immunotoxicol. (IF 3.3) Pub Date : 2022-03-07 Yang Qu, Lin Wang, Yanfang Mao
Abstract Microglia, the main immune effector cells in the central nervous system, play a dual role in the function/structure of the blood–brain barrier (BBB) and brain health. During and soon after a cerebral ischemic injury, microglia produce neurotrophic factors and neurotoxins that can impact on the injury itself and pathology progression. At the same time, microglia undergo polarization to M1 or
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Surgical stress quickly affects the numbers of circulating B-cells and neutrophils in murine septic and aseptic models through a β2 adrenergic receptor J. Immunotoxicol. (IF 3.3) Pub Date : 2022-03-02 Ryutaro Nishioka, Yusuke Nishi, Mohammed E. Choudhury, Riko Miyaike, Ayataka Shinnishi, Kensuke Umakoshi, Yasutsugu Takada, Norio Sato, Mayuki Aibiki, Hajime Yano, Junya Tanaka
Abstract Sepsis is a pathology accompanied by increases in myeloid cells and decreases in lymphoid cells in circulation. In a murine sepsis model induced by cecum ligation and puncture (CLP), increasing numbers of neutrophils and decreasing levels of B-cells in circulation are among the earliest changes in the immune system. However, to date, the mechanisms for these changes remain to be elucidated
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Adaptation of an ELISA assay for detection of IgG2a responses against therapeutic monoclonal antibodies in a mouse immunization model J. Immunotoxicol. (IF 3.3) Pub Date : 2022-01-25 Shraddha S Rane, Rebecca J Dearman, Ian Kimber, Jeremy P Derrick
Abstract Biotherapeutic monoclonal antibodies (mAb) play important roles in clinical medicine but their potential to elicit immune responses in patients remains a major issue. In a study designed to investigate the effect of aggregation on immunogenic responses, mice were immunized with two monoclonal antibodies (mAb1 and mAb2). Serum levels of total IgG, IgG1, and IgG2a were measured by ELISA. An
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Renal tubular cell necroptosis: A novel mechanism of kidney damage in trichloroethylene hypersensitivity syndrome mice J. Immunotoxicol. (IF 3.3) Pub Date : 2021-11-17 Yican Wang, Meng Huang, Xin Du, Yiting Hong, Liping Huang, Yuying Dai, Qifeng Wu, Feng Wang, Qixing Zhu
Abstract Trichloroethylene (TCE) hypersensitivity syndrome (THS), called occupational medicamentosa-like dermatitis due to TCE (OMDT) in China, is a fatal occupational disorder caused by TCE exposure. Visceral damage, including kidney injury, is one of the major complications. Necroptosis is a regulated cell death form linked to local inflammatory response. This study aimed to investigate whether renal
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Impact of injection buffer volume to perform bronchoalveolar lavage fluid collection for isolating alveolar macrophages to investigate fine particle-induced IL-1α secretion J. Immunotoxicol. (IF 3.3) Pub Date : 2021-11-11 Eita Sasaki, Haruka Momose, Keiko Furuhata, Takuo Mizukami, Isao Hamaguchi
Abstract The importance of alveolar macrophages has been reported in many toxicology/immunology studies. Alveolar macrophages release interleukin (IL)-1α as a damage-associated molecular pattern (DAMP) when stimulated by fine particles. However, it is unclear whether cell isolation procedures affect ex vivo particle-induced responses in primary mouse alveolar macrophages (mAM). In this study, effects
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Application of a newly-developed cynomolgus macaque BiTE-mediated cytotoxic T-lymphocyte activity assay to various immunomodulatory agents in vitro J. Immunotoxicol. (IF 3.3) Pub Date : 2021-10-29 Brendon Frank, Hao Guo, Hervé Lebrec, Xiaoting Wang
Abstract The immunotoxic potential of drug candidates is assessed through the examination of results from a variety of in vitro and in vivo immunophenotyping and functional study endpoints in pre-clinical studies. CD8+ cytotoxic T-lymphocyte (CTL) activity impairment by immunosuppressive agents is recognized to be a potentiating factor for decreased antiviral defense and increased cancer risk. A bi-specific
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A T-dependent antibody response evaluation in CD-1 mice after an acute whole-body inhalation exposure to nickel (II) chloride hexahydrate J. Immunotoxicol. (IF 3.3) Pub Date : 2021-10-13 Samuel Buxton, Michael D. Taylor, Jeffrey T. Weinberg, James M. Randazzo, Vanessa L. Peachee, Adriana Oller
Abstract Nickel (Ni) in ambient air may vary regionally with contributions from both natural processes and anthropogenic activities. Exposure to Ni compounds in ambient air above a certain level is associated with acute adverse effects, such as upper respiratory tract irritation, pneumonitis, and chronic adverse effects, such as respiratory cancer. Inhalation reference exposure standards are enacted
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Highly sensitive in vitro cytokine release assay incorporating high-density preculture J. Immunotoxicol. (IF 3.3) Pub Date : 2021-10-13 Shiho Ito, Kyoko Miwa, Chiharu Hattori, Tetsuo Aida, Yoshimi Tsuchiya, Kazuhiko Mori
Abstract Immunostimulatory effects of monoclonal antibodies (mAb) through binding to Fcγ receptors (FcγR) on immune cells are a likely cause of cytokine release syndrome. However, it is difficult to detect the potential risk of FcγR-dependent cytokine release associated with mAb in the current standard cytokine release assays (CRA), including the air-drying solid-phase method using human peripheral
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Safety and efficacy of using heat-killed Lactobacillus plantarum L-137: High-dose and long-term use effects on immune-related safety and intestinal bacterial flora J. Immunotoxicol. (IF 3.3) Pub Date : 2021-09-24 Hiroko Nakai, Shinji Murosaki, Yoshihiro Yamamoto, Michiko Furutani, Rumiko Matsuoka, Yoshitaka Hirose
Abstract Heat-killed Lactobacillus plantarum L-137 (HK L-137) promotes immune function in animals. In healthy people, T-cell proliferation was shown to be enhanced by taking 10 mg HK L-137 daily for 12 weeks. However, the safety and efficacy of higher doses or longer treatments have not yet been investigated in humans. To investigate the high-dose and long-term use effects of HK L-137 on immune-related
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Epicutaneous challenge with protease allergen requires its protease activity to recall TH2 and TH17/TH22 responses in mice pre-sensitized via distant skin J. Immunotoxicol. (IF 3.3) Pub Date : 2021-09-06
Abstract Epicutaneous exposure to allergenic proteins is an important sensitization route for skin diseases like protein contact dermatitis, immunologic contact urticaria, and atopic dermatitis. Environmental allergen sources such as house dust mites contain proteases, which are frequent allergens themselves. Here, the dependency of T-helper (TH) cell recall responses on allergen protease activity
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Exposure to a mixture of 23 chemicals associated with unconventional oil and gas operations alters immune response to challenge in adult mice J. Immunotoxicol. (IF 3.3) Pub Date : 2021-08-28
Abstract The prevalence of unconventional oil and gas (UOG) operations raises concerns regarding the potential for adverse health outcomes following exposure to water tainted by mixtures of UOG associated chemicals. The potential effects that exposure to complex chemical mixtures has on the immune system have yet to be fully evaluated. In this study, effects on the immune system of adult mice exposed
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Dietary advanced glycation end-products elicit toxicological effects by disrupting gut microbiome and immune homeostasis J. Immunotoxicol. (IF 3.3) Pub Date : 2021-08-26
Abstract The aging immune system is characterized by a low-grade chronic systemic inflammatory state (“inflammaging”) marked by elevated serum levels of inflammatory molecules such as interleukin (IL)-6 and C-reactive protein (CRP). These inflammatory markers were also reported to be strong predictors for the development/severity of Type 2 diabetes, obesity, and COVID-19. The levels of these markers
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Immunotoxicity studies of sulfolane following developmental exposure in Hsd:Sprague Dawley SD rats and adult exposure in B6C3F1/N mice J. Immunotoxicol. (IF 3.3) Pub Date : 2021-08-06
Abstract Sulfolane is a solvent used in the petrochemical industry and a groundwater contaminant in areas near refineries. The current studies were conducted to assess the impact of oral exposure to sulfolane on the immune system using two models: (1) a perinatal drinking water exposure to 0, 30, 100, 300, or 1000 mg/L from gestation day (GD) 6 until ∼13 weeks-of-age in Harlan Sprague Dawley rats;
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Serum vaccine antibody concentrations in adults exposed to per- and polyfluoroalkyl substances: A birth cohort in the Faroe Islands J. Immunotoxicol. (IF 3.3) Pub Date : 2021-06-18 Yu-Hsuan Shih, Annelise J. Blomberg, Marie-Abèle Bind, Dorte Holm, Flemming Nielsen, Carsten Heilmann, Pál Weihe, Philippe Grandjean
Abstract Per- and polyfluoroalkyl substances (PFASs) are highly persistent in the environment and may cause depressed immune function. Previous studies have linked PFAS exposure to lower vaccine responses in children, but research in adults is limited. Therefore, the present study evaluated the associations between exposure to PFASs and serum antibody concentrations in adults vaccinated at age 28 years
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Impact of mono-culture vs. Co-culture of keratinocytes and monocytes on cytokine responses induced by important skin sensitizers J. Immunotoxicol. (IF 3.3) Pub Date : 2021-05-21 Venkatanaidu Karri, Carola Lidén, Nanna Fyhrquist, Johan Högberg, Hanna L. Karlsson
Abstract Sensitization to a contact allergen brings with it a lifelong risk to develop allergic contact dermatitis. Inflammation is an important part of the skin sensitizing mechanism, and understanding how different haptens stimulate the immune system, as well as the role played by different cell types present in skin, may be helpful for developing optimized in vitro models for risk assessment of
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Adverse immunological responses against non-viral nanoparticle (NP) delivery systems in the lung J. Immunotoxicol. (IF 3.3) Pub Date : 2021-05-06 Leonor de Braganca, G. John Ferguson, Jose Luis Santos, Jeremy P. Derrick
Abstract There is a large, unmet medical need to treat chronic obstructive pulmonary disease, asthma, idiopathic pulmonary fibrosis and other respiratory diseases. New modalities are being developed, including gene therapy which treats the disease at the DNA/RNA level. Despite recent innovations in non-viral gene therapy delivery for chronic respiratory diseases, unwanted or adverse interactions with
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Adverse immunological responses against non-viral nanoparticle (NP) delivery systems in the lung. J. Immunotoxicol. (IF 3.3) Pub Date : 2021-05-07 Leonor de Braganca,G John Ferguson,Jose Luis Santos,Jeremy P Derrick
There is a large, unmet medical need to treat chronic obstructive pulmonary disease, asthma, idiopathic pulmonary fibrosis and other respiratory diseases. New modalities are being developed, including gene therapy which treats the disease at the DNA/RNA level. Despite recent innovations in non-viral gene therapy delivery for chronic respiratory diseases, unwanted or adverse interactions with immune
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From nicotine to the cholinergic anti-inflammatory reflex – Can nicotine alleviate the dysregulated inflammation in COVID-19? J. Immunotoxicol. (IF 3.3) Pub Date : 2021-04-16 Alex G. Gauthier, Mosi Lin, Jiaqi Wu, Thomas P. Kennedy, Lee-Anne Daley, Charles R. Ashby Jr., Lin L. Mantell
Abstract The coronavirus SARS-CoV-2 of 2019 (COVID-19) causes a pandemic that has been diagnosed in more than 70 million people worldwide. Mild-to-moderate COVID-19 symptoms include coughing, fever, myalgia, shortness of breath, and acute inflammatory lung injury (ALI). In contrast, acute respiratory distress syndrome (ARDS) and respiratory failure occur in patients diagnosed with severe COVID-19.
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Galangin ameliorates experimental autoimmune encephalomyelitis in mice via modulation of cellular immunity J. Immunotoxicol. (IF 3.3) Pub Date : 2021-03-26 Kok-Tong Tan, Shiming Li, Lauren Panny, Chi-Chien Lin, Shih-Chao Lin
Abstract Multiple sclerosis (MS) causes neurologic disabilities that effect musculature, sensory systems, and vision. This is largely due to demyelination of nerve fibers caused by chronic inflammation. Corticosteroid treatments ameliorate symptoms of MS, but do not successfully cure the disease itself. In the current study, the application of galangin, a phytochemical flavonoid extracted from the
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Immune activation by microbiome shapes the colon mucosa: Comparison between healthy rat mucosa under conventional and germ-free conditions J. Immunotoxicol. (IF 3.3) Pub Date : 2021-03-22 Fabián Čaja, Dmitry Stakheev, Oleksander Chernyavskiy, Jiří Křížan, Jiří Dvořák, Pavel Rossmann, Renata Štěpánková, Peter Makovický, Pavol Makovický, Hana Kozáková, Luca Vannucci
Abstract Germ-free animals (GF) are those without a microbiome since birth. This particular biological model has become one of special interest with the growing evidence of importance of the microbiome in the life, development, adaptation, and immunity of humans and animals in the environments in which they live. Anatomical differences observed in GF compared with conventionally-reared animals (CV)
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Effect of inhaled anesthetic gases on immune status alterations in health care workers J. Immunotoxicol. (IF 3.3) Pub Date : 2021-02-22 Ashraf Mahmoud Emara, Khaled Ali Alrasheedi, Salha Dihim Alrashidi, Rehab Mohamed Elgharabawy
Abstract The objective of this research was to evaluate consequences to the immune system of long-term exposure to waste anesthetic gases (WAG) by medical theater personnel. Two groups were recruited: (i) 60 healthy male controls; (ii) 120 medical professionals exposed to WAG, subdivided according to theater role, i.e. surgeons, surgical assistants (SA), anesthetists, anesthetic assistants (AA), nurses
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In vitro prediction of in vivo pseudo-allergenic response via MRGPRX2 J. Immunotoxicol. (IF 3.3) Pub Date : 2021-02-11 Linu M. John, Charlotte M. Dalsgaard, Claus B. Jeppesen, Kilian W. Conde-Frieboes, Katrine Baumann, Niels P. H. Knudsen, Per S. Skov, Birgitte S. Wulff
Abstract In development of peptide therapeutics, rodents are commonly-used preclinical models when screening compounds for efficacy endpoints in the early stages of discovery projects. During the screening process, some peptides administered subcutaneously to rodents caused injection site reactions manifesting as localized swelling. Screening by postmortem evaluations of injection site swelling as
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Local inflammatory marker production in Lithuanian patients with chronic rhinosinusitis with nasal polyps J. Immunotoxicol. (IF 3.3) Pub Date : 2020-12-12 Justinas Vaitkus, Astra Vitkauskiene, Vilte Matuseviciute, Albinas Naudziunas, Nora Siupsinskiene, Saulius Vaitkus
Abstract There are two clinical subtypes of chronic rhinosinusitis (CRS): chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP). The aim of the study here was to determine the levels of invasive inflammatory markers in nasal mucosa samples taken from CRSwNP patients during the surgery and to identify markers that could serve as targets for potential
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Immunotoxicity studies of trans-resveratrol in male B6C3F1/N mice J. Immunotoxicol. (IF 3.3) Pub Date : 2020-11-20 Madelyn C. Huang, Kimber L. White Jr., Susan A. Elmore, Tai L. Guo, Dori Germolec
Abstract Resveratrol is a naturally occurring polyphenol that is being investigated to treat and prevent various diseases, both experimentally and in the clinic. Despite increased use and interest in resveratrol due to its immunomodulatory properties, there is a lack of studies evaluating potential toxicities, particularly immunotoxicity, associated with resveratrol use. A previous 2-week study found
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A T-cell-dependent antibody response study using a murine surrogate anti-PD-1 monoclonal antibody as an alternative to a non-human primate model J. Immunotoxicol. (IF 3.3) Pub Date : 2020-10-20 Lisa M. Plitnick, Beth Hutchins, Sheri Dubey, Nianyu Li, Rupesh P. Amin, Stephanie Born, Ruban Mangadu, Joseph H. Phillips, Venkataraman Sriram, Danuta J. Herzyk
Abstract The programmed cell death 1 (PD-1) pathway represents a major immune checkpoint which may be engaged by cells in a tumor microenvironment to overcome active T-cell immune surveillance. Pembrolizumab (Keytruda®) is a potent and highly selective humanized monoclonal antibody (mAb) of the IgG4/κ isotype designed to directly block the interaction between PD-1 and its ligands, PD-L1 and PD-L2.
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Cadmium induces CCL2 production in glioblastoma cells via activation of MAPK, PI3K, and PKC pathways J. Immunotoxicol. (IF 3.3) Pub Date : 2020-10-19 Thitima Kasemsuk, Suttinee Phuagkhaopong, Ruedeemars Yubolphan, Norapat Rungreangplangkool, Pornpun Vivithanaporn
Abstract Cadmium (Cd) is accumulated in human astrocytes and induces the production of interleukin (IL)-6 and IL-8. Astrocytes are one of the major sources of chemokine C–C motif ligand 2 (CCL2; known as monocyte chemoattractant protein-1 [MCP-1]), in the brain. Elevated CCL2 levels are associated with cognitive impairment as well as the migration and invasion of glioblastoma cells. The present study
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The impact and toxicity of glyphosate and glyphosate-based herbicides on health and immunity. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-09-08 Cindy Peillex,Martin Pelletier
Glyphosate, or N-phosphomethyl(glycine), is an organophosphorus compound and a competitive inhibitor of the shikimate pathway that allows aromatic amino acid biosynthesis in plants and microorganisms. Its utilization in broad-spectrum herbicides, such as RoundUp®, has continued to increase since 1974; glyphosate, as well as its primary metabolite aminomethylphosphonic acid, is measured in soils, water
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Organic dust-induced lung injury and repair: Bi-directional regulation by TNFα and IL-10. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-07-07 T A Wyatt,M Nemecek,D Chandra,J M DeVasure,A J Nelson,D J Romberger,J A Poole
Abstract Exposure to organic dust increases chronic airway inflammatory disorders. Effective treatment strategies are lacking. It has been reported that hog barn dust extracts (HDE) induce TNFα through protein kinase C (PKC) activation and that lung inflammation is enhanced in scavenger receptor A (SRA/CD204) knockout (KO) mice following HDE. Because interleukin (IL)-10 production can limit excessive
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Effect of lead exposure and nutritional iron-deficiency on immune response: A vaccine challenge study in rats. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-06-23 Srinivasa Reddy Yathapu,Narendra Babu Kondapalli,Sarath Babu Srivalliputturu,Rajkumar Hemalatha,Dinesh Kumar Bharatraj
Abstract The prevalence of iron (Fe) deficiency and subclinical lead (Pb) toxicity is high in developing countries like India, and information on their potential additive effects on immune responses is scant. The current study assessed immune parameters in dual Pb-exposed\Fe-deficient weanling SD rats. Rats were fed a control (CD) or Fe-deficient (ID) diet for 4 weeks and then evaluated for hemoglobin
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Prenatal arsenic exposure interferes in postnatal immunocompetence despite an absence of ongoing arsenic exposure. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-06-13 Mainak Chakraborty,Moumita Bhaumik
Abstract Arsenic (As) readily crosses the placenta and exposure of the fetus may cause adverse consequences later in life, including immunomodulation. In the current study, the question was asked how the immune repertoire might respond in postnatal life when there is no further As exposure. Here, pregnant mice (Balb/c [H-2d]) were exposed to arsenic trioxide (As2O3) through their drinking water from
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MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-06-11 Marc A Lafleur,Jonathan Werner,Madeline Fort,Edward K Lobenhofer,Mercedesz Balazs,Ana Goyos
Abstract Mast cells play key roles in allergy, anaphylaxis/anaphylactoid reactions, and defense against pathogens/toxins. These cells contain cytoplasmic granules with a wide spectrum of pleotropic mediators that are released upon activation. While mast cell degranulation (MCD) occurs upon clustering of the IgE receptor bound to IgE and antigen, MCD is also triggered through non-IgE-mediated mechanisms
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Potential classification of chemical immunologic response based on gene expression profiles. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-05-25 Stacey E Anderson,Rachel Baur,Michael Kashon,Ewa Lukomska,Lisa Weatherly,Hillary L Shane
Abstract Occupational immune diseases are a serious public health burden and are often a result of exposure to low molecular weight (LMW) chemicals. The complete immunological mechanisms driving these responses are not fully understood which has made the classification of chemical allergens difficult. Antimicrobials are a large group of immunologically-diverse LMW agents. In these studies, mice were
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In vivo assessment of respiratory burst inhibition by xenobiotic exposure using larval zebrafish. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-05-14 Drake W Phelps,Ashley A Fletcher,Ivan Rodriguez-Nunez,Michele R Balik-Meisner,Debra A Tokarz,David M Reif,Dori R Germolec,Jeffrey A Yoder
Currently, assessment of the potential immunotoxicity of a given agent involves a tiered approach for hazard identification and mechanistic studies, including observational studies, evaluation of immune function, and measurement of susceptibility to infectious and neoplastic diseases. These studies generally use costly low-throughput mammalian models. Zebrafish, however, offer an excellent alternative
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Circulating inflammatory markers in cervical cancer patients and healthy controls. J. Immunotoxicol. (IF 3.3) Pub Date : 2020-05-04 Agne Vitkauskaite,Daiva Urboniene,Joana Celiesiute,Kristina Jariene,Erika Skrodeniene,Ruta Jolanta Nadisauskiene,Daiva Vaitkiene
There is increasing evidence that host inflammatory responses play an important role in the development and progression of cancers. There are some data that cancer is associated not only with inflammation at the site of the lesion, but also with dysregulations of the host overall systemic immune response. In the case of cervical cancer, inflammation is an important factor associated with the development