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Precision Touch DNA Sampling on Plastic Bag Knots for Improved Profiling of Packer and Holder Contributions Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-03-16 Aleksandra Stefanovic, Dejan Šorgić, Nataša Cvetković, Aleksandra Antović, Goran Ilić
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Deconvoluting multi-person biological mixtures and accurate characterization and identification of separated contributors using non-targeted single-cell DNA sequencing Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-03-13 Lucie Kulhankova, Eric Bindels, Manfred Kayser, Eskeatnaf Mulugeta
The genetic characterization and identification of individuals who contributed to biological mixtures are complex and mostly unresolved tasks. These tasks are relevant in various fields, particularly in forensic investigations, which frequently encounters crime scene stains generated by more than one person. Currently, forensic mixture deconvolution is mostly performed subsequent to forensic DNA profiling
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A preliminary study on identification of the blood donor in a body fluid mixture using a novel compound genetic marker blood-specific methylation-microhaplotype Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-03-11 Xuan Tang, Dan Wen, Xin Jin, Chudong Wang, Wei Xu, Weifeng Qu, Ruyi Xu, Hongtao Jia, Yi Liu, Xue Li, Siqi Chen, Xiaoyi Fu, Bin Liang, Jienan Li, Ying Liu, Lagabaiyila Zha
Blood-containing mixtures are frequently encountered at crime scenes involving violence and murder. However, the presence of blood, and the association of blood with a specific donor within these mixtures present significant challenges in forensic analysis. In light of these challenges, this study sought to address these issues by leveraging blood-specific methylation sites and closely linked microhaplotype
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A preliminary report on the exploration of salivary bacterial diversity by the multiplex SNaPshot assay Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-03-08 Shuangshuang Wang, Feng Song, Xiangnan Guo, Liya Gu, Weijia Tan, Peiyan Wu, Weibo Liang, Haibo Luo, Yanyun Wang
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Development and validation of YARN: A novel SE-400 MPS kit for East Asian paternal lineage analysis Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-03-05 Haoliang Fan, Yiran Xu, Yutao Zhao, Kai Feng, Liuxi Hong, Qiancheng Zhao, Xiaoyu Lu, Meisen Shi, Haiyan Li, Lingxiang Wang, Shaoqing Wen
Y-chromosomal short tandem repeat polymorphisms (Y-STRs) and Y-chromosomal single nucleotide polymorphisms (Y-SNPs) are valuable genetic markers used in paternal lineage identification and population genetics. Currently, there is a lack of an effective panel that integrates Y-STRs and Y-SNPs for studying paternal lineages, particularly in East Asian populations. Hence, we developed a novel Y-chromosomal
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Recurrent familial case of early childhood sudden death: Complex post mortem genetic investigations Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-03-02 Lila Krebs-Drouot, Audrey Schalk, Elise Schaefer, Christine Keyser, Angela Gonzalez, Nadège Calmels, Marie-Thérèse Abi Wardé, Laetitia Oertel, C.écile Acquaviva, Jean-Louis Mandel, Audrey Farrugia
Sudden Unexplained Death in Childhood (SUDC) needs to be fully assessed considering its impact on the family, parents and siblings. Inborn Errors of Metabolism (IEM) such as Medium-Chain Acyl-CoA Dehydrogenase Deficiency (MCADD) should be taken into consideration when SUDC occurres. Our aim is to present a family with two successive SUDC and to discuss the post-mortem genetics investigations revealing
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Predicting probative levels of touch DNA on tapelifts using Diamond™ Nucleic Acid Dye. Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-02-04 Isla Madden, Duncan Taylor, Natasha Mitchell, Mariya Goray, Julianne Henry
Tapelifting is a common strategy to recover touch DNA deposits from porous exhibits in forensic DNA casework. However, it is known that only about 30% of tapelifts submitted for DNA analysis in operational forensic laboratories yield profiles suitable for comparison or upload to a searchable database. A reliable means to identify and remove non-probative tapelifts from the workflow would reduce sample
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Sunda pangolin (M. javanica) detected for the first time in the Illegal wildlife seizures from northeast India Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-02-03 Lenrik Konchok Wangmo, Avijit Ghosh, Vinay Kumar Singh, Stanzin Dolker, Dhriti Banerjee, Lalit Kumar Sharma, Mukesh Thakur
Abstract not available
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Investigative use of human environmental DNA in forensic genetics Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-29 Chiara Fantinato, Peter Gill, Ane Elida Fonneløp
Individuals leave behind traces of their DNA wherever they go. DNA can be transferred to surfaces and items upon touch, can be released into the air, and may be deposited in indoor dust. The mere presence of individuals in a location is sufficient to facilitate either direct or indirect DNA transfer into the surrounding environment. In this study, we analyzed samples recovered from commonly touched
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Trace DNA and its persistence on various surfaces: A long term study investigating the influence of surface type and environmental conditions - Part one, metals Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-29 Hilary Arsenault, Agnieszka Kuffel, Niamh Nic Daeid, Alexander Gray
It is imperative for proper evidence triage that forensic biologists understand what kind of results to expect from certain evidence types submitted for DNA analysis. The persistence of trace DNA has been insufficiently investigated and there is little data available pertaining to the persistence of DNA in different environmental conditions and on different materials. The goal of this study is to increase
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Response of salivary microbiome to temporal, environmental, and surface characteristics under in vitro exposure Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-24 Litao Huang, Xiaomin Liang, Guichao Xiao, Jieyu Du, Linying Ye, Qin Su, Chao Liu, Ling Chen
The microbiome of saliva stains deposited at crime scenes and in everyday settings is valuable for forensic investigations and environmental ecology. However, the dynamics and applications of microbial communities in these saliva stains have not been fully explored. In this study, we analyzed saliva samples that were exposed to indoor conditions for up to 1 year and to different carriers (cotton, sterile
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Inference of tobacco and alcohol consumption habits from DNA methylation analysis of blood Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-28 A. Ambroa-Conde, M.A. Casares de Cal, A. Gómez-Tato, O. Robinson, A. Mosquera-Miguel, M. de la Puente, J. Ruiz-Ramírez, C. Phillips, M.V. Lareu, A. Freire-Aradas
DNA methylation has become a biomarker of great interest in the forensic and clinical fields. In criminal investigations, the study of this epigenetic marker has allowed the development of DNA intelligence tools providing information that can be useful for investigators, such as age prediction. Following a similar trend, when the origin of a sample in a criminal scenario is unknown, the inference of
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Harmonizing the forensic nomenclature for STR loci D6S474 and DYS612 Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-21 Martin Bodner, David Ballard, Lisa A. Borsuk, Jonathan L. King, Walther Parson, Christopher Phillips, Katherine Butler Gettings
The autosomal STR D6S474 and the Y-chromosomal STR DYS612 have been reported in multiple ways in the forensic literature, with differences in both the bracketed repeat structures and counting of numerical length-based capillary electrophoresis (CE) alleles. These issues often come to light when STR loci are introduced in commercial assays and results compared with historical publications of allele
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Comprehensive landscape of non-CODIS STRs in global populations provides new insights into challenging DNA profiles Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-20 Yuguo Huang, Mengge Wang, Chao Liu, Guanglin He
The worldwide implementation of short tandem repeats (STR) profiles in forensic genetics necessitated establishing and expanding the CODIS core loci set to facilitated efficient data management and exchange. Currently, the mainstay CODIS STRs are adopted in most general-purpose forensic kits. However, relying solely on these loci failed to yield satisfactory results for challenging tasks, such as bio-geographical
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Sequence-based population structure, relatedness, and inbreeding estimates for forensic autosomal STR markers Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-15 Sanne E. Aalbers, Bruce S. Weir
Population data have become available for sequence data to aid forensic investigations and prepare the forensic community in the move towards implementing NGS methods. This comes with a need for updated population genetic parameters estimates to allow DNA evidence evaluations using sequence data. Initial work has been done on a small sample and here we expand this work by providing estimates of population
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Using simulated microhaplotype genotyping data to evaluate the value of machine learning algorithms for inferring DNA mixture contributor numbers Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-09 Haoyu Wang, Qiang Zhu, Yuguo Huang, Yueyan Cao, Yuhan Hu, Yifan Wei, Yuting Wang, Tingyun Hou, Tiantian Shan, Xuan Dai, Xiaokang Zhang, Yufang Wang, Ji Zhang
Inferring the number of contributors (NoC) is a crucial step in interpreting DNA mixtures, as it directly affects the accuracy of the likelihood ratio calculation and the assessment of evidence strength. However, obtaining the correct NoC in complex DNA mixtures remains challenging due to the high degree of allele sharing and dropout. This study aimed to analyze the impact of allele sharing and dropout
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Age prediction using DNA methylation of Y-chromosomal CpGs in semen samples Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2024-01-06 Ji Eun Lee, Sang Un Park, Moon Hyun So, Hwan Young Lee
In cases of sexual assault, the evidence often exists as a mixture of female and male body fluids, and in many cases, contains a higher proportion of female body fluids than males. In these cases, Y-STR, rather than autosomal STRs, can provide useful information. It becomes very difficult to identify the true suspect if there is no match among known suspects or if a match exists for two or more suspects
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Retrieving complete plastid genomes of endangered Guibourtia timber using hybridization capture for forensic identification and phylogenetic analysis Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-28 Chuanyang Lin, Yang Lu, Shoujia Liu, Zhaoshan Wang, Lihong Yao, Yafang Yin, Lichao Jiao
The high economic value and increased demand for timber have led to illegal logging and overexploitation, threatening wild populations. In this context, there is an urgent need to develop effective and accurate forensic tools for identifying endangered Guibourtia timber species to protect forest ecosystem resources and regulate their trade. In this study, a hybridization capture method was developed
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Identifying distant relatives using benchtop-scale sequencing Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-27 August E. Woerner, Nicole M. Novroski, Sammed Mandape, Jonathan L. King, Benjamin Crysup, Michael D. Coble
The genetic component of forensic genetic genealogy (FGG) is an estimate of kinship, often conducted at genome scales between a great number of individuals. The promise of FGG is substantial: in concert with genealogical records and other nongenetic information, it can indirectly identify a person of interest. A downside of FGG is cost, as it is currently expensive and requires chemistries uncommon
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Development and comparison of forensic interval age prediction models by statistical and machine learning methods based on the methylation rates of ELOVL2 in blood DNA Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-25 Takayuki Yamagishi, Wataru Sakurai, Ken Watanabe, Kochi Toyomane, Tomoko Akutsu
Age estimation can be useful information for narrowing down candidates of unidentified donors in criminal investigations. Various age estimation models based on DNA methylation biomarkers have been developed for forensic usage in the past decade. However, many of these models using ordinary least squares regression cannot generate an appropriate estimation due to the deterioration in prediction accuracy
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An evaluation of the RapidHIT™ ID system for hair roots stained with Diamond™ Nucleic Acid Dye Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-20 Tabarek Aljumaili, Alicia M. Haines
The RapidHIT™ ID (RHID) system was evaluated for its suitability in processing a single hair root to obtain informative DNA profiles. Hair samples were assessed for nuclear DNA prior to DNA analysis using Diamond™ Nucleic Acid Dye (DD) and real-time Extended Depth of Field (EDF) imaging to visualise and count nuclei if present. Hairs were viewed under an Optico N300F LED Fluorescent Microscope and
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Assessing the consistency of shedder status under various experimental conditions Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-19 Linda Jansson, Chiara Siti, Ronny Hedell, Christina Forsberg, Ricky Ansell, Johannes Hedman
Shedder status is defined as the propensity of an individual to leave DNA behind on touched items or surfaces and has been suggested as one of the major factors influencing DNA transfer. However, little is known about whether shedder status is a constant property of an individual across multiple measurements or when the environmental conditions are changed. We have assessed DNA depositions of six males
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Single-cell investigative genetics: Single-cell data produces genotype distributions concentrated at the true genotype across all mixture complexities Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-19 Catherine M. Grgicak, Qhawe Bhembe, Klaas Slooten, Nidhi C. Sheth, Ken R. Duffy, Desmond S. Lun
In the absence of a suspect the forensic aim is investigative, and the focus is one of discerning what genotypes best explain the evidence. In traditional systems, the list of candidate genotypes may become vast if the sample contains DNA from many donors or the information from a minor contributor is swamped by that of major contributors, leading to lower evidential value for a true donor’s contribution
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Differences of circular RNA expression profiles between monozygotic twins' blood, with the forensic application in bloodstain and saliva Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-19 Junyan Wang, Guangping Fu, Qian Wang, Guanju Ma, Zhonghua Wang, Chaolong Lu, Lihong Fu, Xiaojing Zhang, Bin Cong, Shujin Li
Monozygotic twins (MZTs) possess identical genomic DNA sequences and are usually indistinguishable through routine forensic DNA typing methods, which can be relevant in criminal and paternity cases. Recently, novel epigenetic methods involving DNA methylation and microRNA analysis have been introduced to differentiate MZTs. In this study, we explore the potential of using epigenetic markers, specifically
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DNA identification of monozygotic twins Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-13 Hsiao-Lin Hwa, Chun-Yen Lin, Yu-Jen Yu, Adrian Linacre, James Chun-I. Lee
This study details the differentiation of identical twins based on single mutational base differences. There were three pairs of male monozygotic (MZ) twins in this study. DNA samples from blood, a buccal swab or saliva from each individual were all initially genotyped using 22 autosomal STR and 27 Y-STR loci. Preliminary screening confirmed there were no differences in the STR data between each pair
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Towards a more supportive and inclusive forensic science community Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-11 Vanessa Lynch
Abstract not available
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Microsatellites’ mutation modeling through the analysis of the Y-chromosomal transmission: Results of a GHEP-ISFG collaborative study Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-14 Sofia Antão-Sousa, Leonor Gusmão, Nidia M. Modesti, Sofía Feliziani, Marisa Faustino, Valeria Marcucci, Claudia Sarapura, Julyana Ribeiro, Elizeu Carvalho, Vania Pereira, Carmen Tomas, Marian M. de Pancorbo, Miriam Baeta, Rashed Alghafri, Reem Almheiri, Juan José Builes, Nair Gouveia, German Burgos, Maria de Lurdes Pontes, Adriana Ibarra, Nadia Pinto
The Spanish and Portuguese Speaking Working Group of the International Society for Forensic Genetics (GHEP-ISFG) organized a collaborative study on mutations of Y-chromosomal short tandem repeats (Y-STRs). New data from 2225 father-son duos and data from 44 previously published reports, corresponding to 25,729 duos, were collected and analyzed. Marker-specific mutation rates were estimated for 33 Y-STRs
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Salmon sperm DNA increases sample recovery from cotton swabs Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-04 Crystal M. Oechsle, Thomas A. Paul, Joseph D. Seichko, Travis J. Worst
Forensic samples with low DNA template amounts are difficult to analyze and interpret. There is a large body of research demonstrating that adding carrier nucleic acid to storage tubes, solid phase extractions, or filtering devices can improve yields of target DNA. However, the addition of carrier nucleic acid to sampling substrates, like cotton swabs, has not yet been attempted. In this proof-of-concept
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Systematic evaluation of the Precision ID GlobalFiler™ NGS STR panel v2 using single-source samples of various quantity and quality and mixed DNA samples Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-02 Vishakha Sharma, Elisa Wurmbach
Massively parallel sequencing (MPS) techniques were developed approximately 15 years ago. Meanwhile, several MPS kits for forensic identification, phenotypic information, ancestry, and mitochondrial DNA analysis have been developed and their use has been established. Sequencing short tandem repeats (STRs) has certain advantages over the currently used length-based genotyping methods, which are based
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Machine learning applications in forensic DNA profiling: A critical review Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-12-01 Mark Barash, Dennis McNevin, Vladimir Fedorenko, Pavel Giverts
Machine learning (ML) is a range of powerful computational algorithms capable of generating predictive models via intelligent autonomous analysis of relatively large and often unstructured data. ML has become an integral part of our daily lives with a plethora of applications, including web, business, automotive industry, clinical diagnostics, scientific research, and more recently, forensic science
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Mixture detection with Demixtify Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-25 August E. Woerner, Benjamin Crysup, Jonathan L. King, Nicole M. Novroski, Michael D. Coble
The de facto genetic markers of forensics are short tandem repeats (STRs). There are many analytical tools designed to work with STRs, including techniques for analyzing and assessing DNA mixtures. In contrast, the nascent field of forensic genetic genealogy often relies on biallelic single nucleotide polymorphisms (SNPs). Tools designed for the forensic assessment of SNPs are somewhat lacking, especially
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An mRNA profiling assay incorporating coding region InDels for body fluid identification and the inference of the donor in mixed samples Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-28 Shouyu Wang, Tingting Jiang, Chunyan Yuan, Liming Wu, Xiaoyuan Zhen, Yinlei Lei, Baoyan Xie, Ruiyang Tao, Chengtao Li
Biological traces discovered at crime scenes hold significant significance in forensic investigations. In cases involving mixed body fluid stains, the evidentiary value of DNA profiles depends on the type of body fluid from which the DNA was obtained. Recently, coding region polymorphism analysis has proved to be a promising method for directly linking specific body fluids to their respective DNA contributors
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Metatranscriptomic characterization of six types of forensic samples and its potential application to body fluid/tissue identification: A pilot study Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-17 Zhiyong Liu, Jiajun Liu, Jiaojiao Geng, Enlin Wu, Jianzhang Zhu, Bin Cong, Riga Wu, Hongyu Sun
Microorganisms are potential markers for identifying body fluids (venous and menstrual blood, semen, saliva, and vaginal secretion) and skin tissue in forensic genetics. Existing published studies have mainly focused on investigating microbial DNA by 16 S rRNA gene sequencing or metagenome shotgun sequencing. We rarely find microbial RNA level investigations on common forensic body fluid/tissue. Therefore
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Selecting mRNA markers in blood for age estimation of the donor of a biological stain Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-15 Guro Dørum, Nadescha Viviane Hänggi, Dario Burri, Yael Marti, Regine Banemann, Galina Kulstein, Cornelius Courts, Annica Gosch, Thorsten Hadrys, Cordula Haas, Jacqueline Neubauer
RNA has gained a substantial amount of attention within the forensic field over the last decade. There is evidence that RNAs are differentially expressed with biological age. Since RNA can be co-extracted with DNA from the same piece of evidence, RNA-based analysis appears as a promising molecular alternative for predicting the biological age and hence inferring the chronological age of a person. Using
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Determining the number and size of background samples derived from an area adjacent to the target sample that provide the greatest support for a POI in a target sample Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-10 Jack B. Reither, Duncan Taylor, Bianca Szkuta, Roland A.H. van Oorschot
When sampling an item or surface for DNA originating from an action of interest, one is likely to collect DNA unrelated to the action of interest (background DNA). While adding to the complexity of a generated DNA profile, background DNA has been shown to aid in resolving the genotypes of contributors in a targeted sample, and where references of donors to the background DNA are not available, strengthen
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Visualisation and detection of latent DNA deposited by pangolin scales onto plastic packaging materials Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-07 Amy H.J. Chan, Michael G. Gardner, Adrian Linacre
We report on the detection and visualisation of latent DNA from pangolin scales deposited onto a plastic packaging material through the use of a nucleic acid staining dye. This latent DNA deposited by pangolin scales was subsequently isolated and analysed using DNA barcoding method. Pangolins are the most illegally traded mammalian species due to the demand for their scales and meat. The demand for
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Analysis of 12 X-STRs loci in a population from southeastern Brazil Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-11-02 Fernanda M. Garcia, Raquel S.R. Trabach, Victor S. Stange, Bruno Cancian, Alexia S.S. Zetum, Matheus C. Casotti, Flavia I.V. Errera, Elizeu F. de Carvalho, Débora D. Meira, Iuri D. Louro
Short tandem repeat (STR) markers on the X chromosome have a high potential for solving complex kinship analysis and individual identification cases. To achieve such purposes, allele and haplotype frequencies for the specific population are necessary. Nonetheless, such frequencies are not always available. Therefore, we obtained haplotypes from 520 unrelated males from four different geographic regions
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Operationalisation of the ForenSeq® Kintelligence Kit for Australian unidentified and missing persons casework Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-30 Jessica Watson, Dennis McNevin, Kelly Grisedale, Michelle Spiden, Shelley Seddon, Jodie Ward
Single nucleotide polymorphism (SNP) genotyping technologies can generate investigative leads for human remains identification, including estimation of biological sex, biogeographical ancestry (BGA), externally visible characteristics (EVCs), identity, uniparental lineage and extended kinship. The ForenSeq® Kintelligence Kit provides forensic laboratories with the ability to apply this suite of genetic
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Estimation of population specific values of theta for sequence-based STR profiles Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-28 Yao-Yuan Liu, Jo-Anne Bright, Duncan Taylor, Maarten Kruijver, John Buckleton
We describe the estimation of θ (theta) values from autosomal STR sequencing data for five metapopulations. The data were compiled from 20 publications and included 39 datasets comprising a total of 7005 samples. The estimates are suitable for use within the calculation of match probabilities in forensic casework. We also have constructed a phylogenetic tree using this data that aligns with our understanding
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Characterization and evaluation of nine Cannabis sativa chloroplast SNP markers for crop type determination and biogeographical origin on European samples Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-24 Michele Di Nunzio, Carme Barrot-Feixat, David Gangitano
Cannabis sativa can be classified in two main types, according to psychotropic cannabinoid ∆9-tetrahydrocannabinol (∆9-THC) content: the drug-type and the fiber-type. According to the European Monitoring Center for Drugs and Drug Addiction, most of the European Union countries consider the possession of cannabis, for personal use, a minor offense with possibility of incarceration. Despite of the model
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A proof-of-principle study: The potential application of MiniHap biomarkers in ancestry inference based on the QNome nanopore sequencing Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-17 Jing Liu, Suyu Li, Yonglin Su, Yufeng Wen, Liu Qin, Mengyao Zhao, Minxiao Hui, Lirong Jiang, Xiacan Chen, Yiping Hou, Zheng Wang
Haplotyped SNPs convey forensic-related information, and microhaplotypes (MHs), as the most representative of this kind of marker, have proved the potential value for human forensics. In recent years, nanopore sequencing technology has developed rapidly, with its outstanding ability to sequence long continuous DNA fragments and obtain phase information, making the detection of longer haplotype marker
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Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on short tandem repeat sequence nomenclature Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-18 Katherine B. Gettings, Martin Bodner, Lisa A. Borsuk, Jonathan L. King, David Ballard, Walther Parson, Corina C.G. Benschop, Claus Børsting, Bruce Budowle, John M. Butler, Kristiaan J. van der Gaag, Peter Gill, Leonor Gusmão, Douglas R. Hares, Jerry Hoogenboom, Jodi Irwin, Lourdes Prieto, Peter M. Schneider, Marielle Vennemann, Christopher Phillips
The DNA Commission of the International Society for Forensic Genetics (ISFG) has developed a set of nomenclature recommendations for short tandem repeat (STR) sequences. These recommendations follow the 2016 considerations of the DNA Commission of the ISFG, incorporating the knowledge gained through research and population studies in the intervening years. While maintaining a focus on backward compatibility
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DNA identification from dental pulp and cementum Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-14 Yi-Feng Wei, Chun-Yen Lin, Yu-Jen Yu, Adrian Linacre, James Chun-I. Lee
Teeth are one of the body tissues remaining after severe decomposition from which a DNA profile can be obtained to aid in human identification. Currently, the standard approach to isolate DNA from teeth requires pulverizing the entire tooth. This destructive approach compromises any further morphological or anthropological study. We report on two methods of DNA isolation that minimizes destruction
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Improved age estimation from semen using sperm-specific age-related CpG markers Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-05 Chao Xiao, Ya Li, Maomin Chen, Shaohua Yi, Daixin Huang
Accurate age estimation from semen has the potential to greatly narrow the pool of unidentified suspects in sexual assault investigations. However, previous efforts utilizing semen age-related CpG (AR-CpG) markers have shown lower accuracy compared to blood AR-CpG-based methods. This discrepancy may be attributed to DNA methylation (DNAm) interferences from “round cells” such as leukocytes and immature
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Defining cat mitogenome variation and accounting for numts via multiplex amplification and Nanopore sequencing Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-06 Emily C. Patterson, Gurdeep Matharu Lall, Rita Neumann, Barbara Ottolini, Federico Sacchini, Aiden P. Foster, Mark A. Jobling, Jon H. Wetton
Hair shed by domestic cats is a potentially useful source of forensic evidence. Analysable hair DNA is predominantly mitochondrial, but the recent domestication history of cats means that mtDNA diversity is low. A 402-bp control region segment is usually sequenced, defining only a small number of distinct haplotypes in populations. Previously, we used a long-amplicon approach to sequence whole mitogenomes
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From Suspicions to Certainty: Wildlife DNA Forensics Reveals Canine Cannibalism" Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-06 Vinaya Kumar Singh, Bheem Dutt Joshi, Avijit Ghosh, Sutithi Mitra, Dhriti Banerjee, Lalit Kumar Sharma, Mukesh Thakur
Abstract not available
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Analysis of the influence of host lifestyle (coffee consumption, drinking, and smoking) on Korean oral microbiome Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-10-05 Kyeong-Min Yu, Hye-Seon Cho, A.-Mi Lee, Ji-Woo Lee, Si-Keun Lim
If a DNA sample collected in the field is old or degraded, short tandem repeat analysis is difficult to perform, a representative analysis method currently used for individual identification. Given that microorganisms exist everywhere and within the human body, in similar amounts to human cells, microbial analysis could be used to identify individuals even in cases in which human DNA-based identification
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"Total Human DNA Sampling" – Forensic DNA profiles from large areas Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-28 Colino Neves, Martin Zieger
Employed for the first time in 1986, DNA profiling is nowadays established as one of the most widely used forensic techniques worldwide. However, until today, no efficient sampling technique existed to collect DNA from human skin cells from a large area, not to say from the floor of an entire room. This has been extremely unfortunate, as there is enormous forensic potential in these DNA traces from
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Development of an epigenetic age predictor for costal cartilage with a simultaneous somatic tissue differentiation system Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-29 A. Freire-Aradas, M. Tomsia, D. Piniewska-Róg, A. Ambroa-Conde, MA Casares de Cal, A. Pisarek, A. Gómez-Tato, J. Álvarez-Dios, E. Pośpiech, W. Parson, M. Kayser, C. Phillips, W. Branicki
Age prediction from DNA has been a topic of interest in recent years due to the promising results obtained when using epigenetic markers. Since DNA methylation gradually changes across the individual’s lifetime, prediction models have been developed accordingly for age estimation. The tissue-dependence for this biomarker usually necessitates the development of tissue-specific age prediction models
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Application of array-based age prediction models to post-mortem tissue samples Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-30 Jeong Min Lee, Sang Un Park, Soong Deok Lee, Hwan Young Lee
Since DNA methylation at specific CpG sites exhibits a strong age association, researchers have developed numerous age prediction models based on the methylation BeadChip array. These models harness epigenetic clocks that hold the potential to narrow down the search range for unknown suspects and unidentified victims. This study collected 180 post-mortem tissue samples comprising nine tissue types
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Phenotype predictions of two-person mixture using single cell analysis Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-30 Marta Diepenbroek, Birgit Bayer, Katja Anslinger
Over a decade after the publication of the first forensic DNA phenotyping (FDP) studies, DNA-based appearance predictions are now becoming a reality in routine crime scene investigations. The significant number of publications dedicated to the subject of FDP clearly demonstrates a sustained interest and a strong need for further method development. However, the implementation of FDP in routine work
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Adapting an established Ampliseq microhaplotype panel to nanopore sequencing through direct PCR Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-28 L. Casanova-Adán, A. Mosquera-Miguel, J. González-Bao, A. Ambroa-Conde, J. Ruiz-Ramírez, A. Cabrejas-Olalla, E. González-Martín, A. Freire-Aradas, A. Rodríguez-López, C. Phillips, MV Lareu, M. de la Puente
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The bear necessities: A sensitive qPCR assay for bear DNA detection from bile and derived products to complement wildlife forensic enforcement Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-26 Ashley Friedenberger, Colleen Doyle, Lindsey Couillard, Christopher J. Kyle
Demand for bear bile, a prized component of traditional Asian medicines, threaten Asiatic and sun bear population sustainability. While laws exist to prevent poaching and trafficking of bear parts and derivatives, smuggling persists with demand extending to surrogate species, including American black bears (Ursus americanus). Mitochondrial DNA (mtDNA) sequencing can identify products putatively containing
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Pitfalls and challenges with population assignments of individuals from admixed populations: Applying Genogeographer on Brazilian individuals Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-11 Zehra Köksal, Olivia Luxford Meyer, Jeppe Dyrberg Andersen, Leonor Gusmão, Helle Smidt Mogensen, Vania Pereira, Claus Børsting
The assignment of individuals to a population can be of importance for the identification of mass disaster victims or criminal offenders in the field of forensic genetics. This assignment is based on biostatistical methods that process data of ancestry informative markers (AIMs), which are selected based on large allele frequency differences between the populations of interest. However, population
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Effect existence of aging on stutter ratio evaluated via Bayesian inference Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-09 Shota Inokuchi, Hiroaki Nakanishi, Aya Takada, Kazuyuki Saito
The stochastic behavior of the stutter ratio (SR) in capillary electrophoresis-based DNA typing is currently described and predicted using statistical models in forensic genetics. Clarifying this behavior can help obtain more objective and robust evidence to the court in terms of mixture interpretation. This study aimed to investigate the effect existence of aging on SR via a Bayesian framework. Nail
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Extraction of DNA from trace forensic samples with a modified lysis buffer and chitosan coated magnetic beads Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-09-09 Anzhong Hu, Lin Chen, Xuelei Geng, Ling Zhu, Yong Liu, Ke Yang, Huaqing Zhu, Cancan Zhu
The trace amounts of human tissue cells or body fluids left at the crime scene are often mixed with inhibitors such as rust, pigments, and humic acid. The extraction of the DNA from the trace cells is crucial for the investigation of cases. Usually, specially designed magnetic nanoparticles were chosen by the case investigators to enrich and elute DNA, which was then used for polymerase chain reaction
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The secret hidden in dust: Assessing the potential to use biological and chemical properties of the airborne fraction of soil for provenance assignment and forensic casework Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-08-22 Nicole R. Foster, Duncan Taylor, Jurian Hoogewerff, Michael G. Aberle, Patrice de Caritat, Paul Roffey, Robert Edwards, Arif Malik, Michelle Waycott, Jennifer M. Young
The airborne fraction of soil (dust) is both ubiquitous in nature and contains localised biological and chemical signatures, making it a potential medium for forensic intelligence. Metabarcoding of dust can yield biological communities unique to the site of interest, similarly, geochemical analyses can uncover elements and minerals within dust that can be matched to a geographic location. Combining
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CmVCall: An automated and adjustable nanopore analysis pipeline for heteroplasmy detection of the control region in human mitochondrial genome Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-08-14 Lirong Jiang, Jing Liu, Suyu Li, Yufeng Wen, Xinyue Zheng, Liu Qin, Yiping Hou, Zheng Wang
Genetic associations between human mitochondrial DNA (mtDNA) heteroplasmy and mitochondrial diseases, aging, and cancer have been elaborated, contributing a lot to the further understanding of mtDNA polymorphic spectrum in anthropology, population, and forensic genetics. In the past decade, heteroplasmy detection using Sanger sequencing and next generation sequencing (NGS) was hampered by the former’s
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Comparison of three DNA extraction methods tested on illicit drug-related powders Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-08-10 Amy Griffin, K. Paul Kirkbride, Julianne Henry, Ben Painter, Adrian Linacre
The detection of human DNA on and within illicit drug preparations is novel and a focus of current research. Previous studies have indicated that certain drug-related powders present in illicit drug preparations can interfere with downstream DNA analysis when directly added to the PCR. Therefore, it is important to determine if these drug-related powders are effectively removed during the DNA extraction
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Comparative analysis of two Rapid DNA technologies for the processing of blood and saliva-based samples Forensic Sci. Int. Genet. (IF 3.1) Pub Date : 2023-08-08 Nancy Laurin, Hélène Boulianne, Chantal Frégeau
Rapid DNA technologies recently gained significant momentum as a means to generate DNA profiles faster than with standard laboratory workflows. Initially developed for the analysis of buccal reference samples, applications are being considered for other types of forensic samples. In this study, an identical set of 150 blood and saliva-based samples was processed using two different Rapid DNA technologies