样式: 排序: IF: - GO 导出 标记为已读
-
Plasma metabolomics identifies S-adenosylmethionine as a biomarker and potential therapeutic target for vascular aging in older adult males J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-12 Zhenghui Gu, Yujia Wang, Zhiyi Fang, Tianhu Wang, Shan Gao, Qian Yang, Yingjie Zhang, Yabin Wang, Linghuan Wang, Li Fan, Feng Cao
Brachial-ankle pulse wave velocity (baPWV) is a noninvasive index of vascular aging. However, the metabolic profile underlying vascular aging has not yet been fully elucidated. The current study aimed to identify circulating markers of vascular aging as assessed by baPWV and to elucidate its mechanism from a metabolomic perspective in older adults. A total of 60 and 61 Chinese male participants aged
-
Quantum dots-based multiplexed immunosensors for accurate diagnosis of attention deficit hyperactivity disorder in childhood J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-11 Xinyue Shao, Zhao Dong, Shuai Zhang, Yunyun Qiao, Hongwei Zhang, Hua Guo
Attention-deficit/hyperactivity disorder (ADHD) lacks objective diagnostic markers. In clinical settings, reliance on subjective judgments can often lead to missed or misdiagnoses. Some biomarkers have been reported to be associated with ADHD, but using one biomarker alone is not enough. To address this, we developed a fluorescent immunoassay platform based on quantum dots (QDs) to detect assay capable
-
Quantification of endogenous Angiotensin 1-10, 1-9, 1-8, 1-7, and 1-5 in human plasma using micro-UHPLC-MS/MS: outlining the importance of the pre-analytics for reliable results J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-09 Jonathan Maurer, Anke de Groot, Léon Martin, Eric Grouzmann, Grégoire Wuerzner, Philippe J. Eugster
Angiotensin peptides (ANGs) play a central role in the renin–angiotensin–aldosterone system, rendering them interesting biomarkers associated with hypertension. Precise quantification of circulating ANGs holds the potential to assess the activity of angiotensin-converting enzyme (ACE), a key protease targeted by widely prescribed drugs, namely ACE inhibitors. This ability could pave the way for personalised
-
NPS–EQA PART II: four years’ experience in external quality assessment program in Italy for classical and new psychoactive substances analysis in hair. J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-08 Maria Concetta Rotolo, Silvia Graziano, Maria Rosaria Varì, Adele Minutillo, Alessandro Di Giorgi, Valeria Aquilina, Simona Pichini, Emilia Marchei
In 2019, the Italian National Institute of Health established an external quality assessment (EQA) program to evaluate the performance of laboratories of collaborative centres participating in the National Early Warning System in hair testing for classical and new psychoactive substances (NPS). The results obtained in the four rounds (2019–2023) and the evolution in hair testing performance for classic
-
Network pharmacology analysis and experimental validation of Xiao-Qing-Long-Tang’s therapeutic effects against neutrophilic asthma J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-08 Zhifeng Chen, Yaoliang Zhou, Yuanfei Tan, Sheng-dong He, Xiaoying Ji, Bing Xiao, Hongda Chen
Xiao-Qing-Long-Tang (XQLT), a classical Chinese herbal medicine formula, has been extensively used for allergic asthma treatment. However, there is limited research on its anti-inflammatory effects and mechanisms specifically in neutrophilic asthma (NA). This study aims to investigate the potential therapeutic effects of XQLT against NA using a combination of network pharmacology and experimental validation
-
Simultaneous monitoring of seven antiepileptic drugs by dried blood spot and dried plasma spot sampling: method validation and clinical application of a LC–MS/MS-based technique J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-07 Haiwei Cao, Yi Jiang, Qiang Sun, Ruichen Liu, Yanyan Li, Jing Huang
Alternative blood sampling strategy can enhance the application of therapeutic drug monitoring (TDM), then improve precision therapy and medication compliance. In developing nations, alternative sampling strategy that allows self-sampling and room temperature transport is especially important. This study validates the use of dried blood spot (DBS) and dried plasma spot (DPS) sampling along with liquid
-
Drug Deconjugation-assisted Peptide Mapping by LC-MS/MS to Identify Conjugation Sites and Quantify Site Occupancy for Antibody-drug Conjugates J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-07 Tongdan Wang, Zi-Ao Huang, Moyin Zhou, Ruxin Wang, Yufei Li, Longyun Guo, Xiaolin Cao, Jincui Huang
Antibody-drug conjugates (ADCs) are a heterogeneous mixture of conjugated species with varied drug loadings. Depending on conjugation sites, linkers and drugs can exhibit different stability as influenced by the solvent-accessibility and local charge, resulting in different ADC efficacy, pharmacokinetics, and toxicity. Conjugation site analysis is critical for ADC structural characterization to assure
-
Solid state NMR study of the bioenhancer piperine, supported by GIPAW DFT calculations J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-07 Paweł Siudem, Łukasz Szeleszczuk, Katarzyna Paradowska
Piperine, an alkaloid found in black pepper fruits, has the properties of promoting the absorption of other substances (e.g. curcumin), therefore it is used in solid forms of dietary supplements as an additive increasing bioavailability. The aim of the study was to analyze piperine in a solid state and then to use it in the analysis of solid dietary supplements with the addition of piperine as an absorption
-
High-throughput method for Peptide mapping and Amino acid sequencing for Calcitonin Salmon in Calcitonin Salmon injection using Ultra High Performance Liquid Chromatography – High Resolution Mass Spectrometry (UHPLC-HRMS) with the application of Bioinformatic tools J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-07 Akhilesh Kumar Kuril, K. Saravanan
Tandem mass spectrometry (MS/MS) can provide direct and accurate sequence characterization of synthetic peptide drugs, and peptide drug products including side chain modifications in the Peptide drugs. This article explains a step-by-step guide to developing a high-throughput method using high resolution mass spectrometry for characterization of Calcitonin Salmon injection containing high proportion
-
Multiplexed quantification of venlafaxine and metabolites in human plasma by liquid chromatography-tandem mass spectrometry J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-07 Aashish Pandey, Amelia Price, Nadia Ayala-Lopez, Kyana Y. Garza, Mark A. Marzinke, Claire E. Knezevic
Venlafaxine (VEN) and its O-demethylated metabolite, -desmethylvenlafaxine (ODV), are commonly prescribed serotonin-norepinephrine reuptake inhibitors, approved for the treatment of depression and anxiety. Both are metabolized to inactive metabolites via cytochrome P450 enzymes. While previous studies have focused on quantifying VEN and ODV, bioanalytical methods for the simultaneous measurement of
-
Impurity assessment, development and validation of an RP-HPLC method for the determination of eleven potential impurities of eltrombopag precursor J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-06 Timur Demirhan, Elif Guksu, Yücel Yazar, Elif Keskin, Esen Bellur Atici, Sibel A. Özkan
Eltrombopag is an oral non-peptide thrombopoietin receptor (TPO-R) agonist indicated for the treatment of thrombocytopenia in patients with persistent or chronic immune thrombocytopenia (idiopathic thrombocytopenic purpura, ITP) or chronic hepatitis C infection and the treatment of severe aplastic anemia. The purpose of this research was to assess the possible impurities that may carry over to eltrombopag
-
Ultrasensitive and label-free electrochemical immunosensor for the detection of the ovarian cancer biomarker CA125 based on CuCo-ONSs@AuNPs nanocomposites J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-05 Wendi Mu, Chunyan Wu, Fangfang Wu, Hongmin Gao, Xinshui Ren, Jing Feng, Meng Miao, Hehua Zhang, Dong Chang, Hongzhi Pan
Cancer antigen 125 (CA125) is pivotal as a tumor marker in early ovarian cancer prevention and diagnosis. In this work, we introduced an ultrasensitive label-free electrochemical immunosensor tailored for CA125 detection, leveraging nanogold-functionalized copper-cobalt oxide nanosheets (CuCo-ONSs@AuNPs) as nanocomposites. For the inaugural application, copper-cobalt oxide nanosheets delivered the
-
A multicomponent LC-MS/MS method for drugs of abuse testing using volumetric DBS and a clinical evaluation by comparison with urine J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-03 Joar Guterstam, Christoffer Tavic, Miguel Barosso, Olof Beck
Drug testing commonly use urine as a specimen and immunoassays for screening. The need for supervised urine collection has led to an interest in alternative specimens and a need for using mass spectrometry methods already for screening. In addition, mass spectrometry methods allow for broad multipanel screening which of great value because of the increased number of substances that needs to be covered
-
Integrated component identification, network pharmacology, and experimental verification revealed mechanism of Dendrobium officinale Kimura et Migo against lung cancer J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-03 Yan Zhao, Shuaitao Zhu, Yuan Li, Xuan Niu, Guanxiong Shang, Xiaoqi Zhou, Jiu Yin, Beihua Bao, Yudan Cao, Fangfang Cheng, Zhipeng Li, Ran Wang, Weifeng Yao
Kimura et Migo (DO), a valuable Chinese herbal medicine, has been reported to exhibit potential effects in the prevention and treatment of lung cancer. However, its material basis and mechanism of action have not been comprehensively analyzed. The objective of this study was to preliminarily elucidate the active components and pharmacological mechanisms of DO in treating lung cancer, according to UPLC-Q/TOF-MS
-
A validated LC-MS/MS method for the quantitation of daratumumab in rat serum using rapid tryptic digestion without IgG purification and reduction J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-02 Weiqiang Li, Wensi Huang, Xiong Yu, Chong Chen, Yali Yuan, Donghui Liu, Feiyu Wang, Jinghua Yu, Xingxing Diao
Daratumumab, a humanized monoclonal antibody utilized in treating immunoglobulin light-chain amyloidosis and relapsed/refractory multiple myeloma, was quantified in rat serum through a simple, economical and effective liquid chromatography tandem-mass spectrometry (LC-MS/MS) method. A surrogate peptide, LLIYDASNR, derived from trypsin hydrolysis, was quantitatively analyzed with LLIYDASN [C, N] RAT
-
A metabolomics footprinting approach using GC-MS to study inhibitory effects of the fungal metabolite diplopyrone C against nosocomial pathogen biofilms J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-02 Maria Michela Salvatore, Angela Maione, Marianna Imparato, Francesco Salvatore, Marco Guida, Emilia Galdiero, Anna Andolfi
Seen initially as wonder drugs, the widespread and often inappropriate use of antibiotics led to the development of microbial resistances. As a result, a true emergency has arisen, and a significant need has emerged to discover and develop new safe and valuable antibiotics. The captivating chemical structure of the fungal metabolite diplopyrone C has caught our attention as an excellent candidate for
-
NPS–EQA PART I: Four years’ experience in external quality assessment program in Italy for classical and new psychoactive substances analysis in oral fluid J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-02 Emilia Marchei, Silvia Graziano, Maria Rosaria Varì, Adele Minutillo, Valeria Aquilina, Simona Pichini, Maria Concetta Rotolo
In 2019, Italian National Institute of Health established an external quality assessment program (EQA) to evaluate the performance of oral fluid testing for classical and new psychoactive substances by laboratories participating in the National Early Warning System collaborative centres. This report presents the results of four rounds between 2019 and 2023. Eleven oral fluid specimens, including 3
-
Alterations in serum metabolic profiles of early-stage hepatocellular carcinoma patients after radiofrequency ablation therapy J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-02 Fengmei Zhang, Jing Wu, Lei Zhang, Jian Zhang, Rui Yang
To investigate the alterations in serum metabolic profiles and early-stage hepatocellular carcinoma (HCC) patient characteristics after radiofrequency ablation (RFA) therapy. This evaluation aimed to assess treatment effectiveness and identify potential novel approaches and targets for HCC treatment and prognosis monitoring. Untargeted metabolomics technology was employed to analyze serum metabolic
-
Simultaneous determination of iguratimod and its metabolite in rat plasma using a UPLC-MS/MS method: Application for drug-drug interaction J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-02 Lu Shi, Jinyu Hu, Hualu Wu, Yuxin Shen, Xiaohai Chen, Qinghua Weng, Ren-ai Xu, Congrong Tang
This aim of the work was to establish an acceptable sensitive assay based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for quantitatively analyzing the plasma concentrations of iguratimod (IGR) and its metabolite M2 in rats, and to further investigate the effect of fluconazole on the pharmacokinetics of IGR and M2. The mobile phase consisted of acetonitrile and water
-
Testing for Kratom alkaloids in fingernail clippings – not only mitragynine J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-03-01 Alice Ameline, Laurie Gheddar, Nadia Arbouche, Adeline Blanchot, Jean-Sebastien Raul, Pascal Kintz
Kratom () is a species of large tree that grows in Southeast Asia and is part of the Rubiaceae family. Its fresh leaves are harvested for their medicinal properties and used for their psychoactive effects. Kratom contains many biologically active alkaloids, including mitragynine and 7-OH-mitragynine, which are considered the two most important psychoactive components and constitute approximately 66%
-
Determination of four aminoglycoside antibiotics and spectinomycin in feed at cross-contamination level: Development and in-house validation of a LC–MS/MS method for enforcing EU Regulation J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-27 Luca Ferrari, Carlos Gonçalves, Joerg Stroka, Christoph von Holst, Luciano Pinotti, Ursula Vincent
Combating antimicrobial resistance is a top priority worldwide involving a concerted action by several high-level institutions and organisations in the health sector. To ensure that a meaningful progress is achieved, several campaigns and political initiatives have been launched targeting the health professionals, the industry, the farmers, and the general public. The Regulation (EU) 2019/4 on medicated
-
Development and functionalization of electrospun fiber coated thin film microextraction devices for rapid mass spectrometric determination of biologically important polar molecules J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-27 Merve Öztürk, Bekir Salih, Ahmet E. Eroğlu, Ezel Boyaci
Rapid diagnosis of diseases is one of the challenging areas in clinical research. From the analytical chemist’s perspective, the main challenges are isolating the compounds from the bio-specimen and lengthy analysis times. In this regard, solid phase microextraction offers a platform to address the abovementioned challenges. Moreover, its sharp tip-thin film geometry, known as coated blade spray (CBS)
-
Optimization of enantioselective high-performance liquid chromatography-tandem mass spectrometry method for the quantitative determination of 3,4-methylenedioxy-methamphetamine (MDMA) and its phase-1 metabolites in human biological fluids J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-27 Giorgia Sprega, Giorgi Kobidze, Alfredo Fabrizio Lo Faro, Simona Pichini, Tivadar Farkas, Anastasio Tini, Antonina Mskhiladze, Francesco Paolo Busardò, Bezhan Chankvetadze
Recently we published in this journal an enantioselective high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitative determination of 3,4-methylenedioxymethamphetamine (MDMA) and its major phase-1 metabolites 4-hydroxy-3-methoxyamphetamine (HMA), 4-hydroxy-3-methoxymethamphetamine (HMMA) and 3,4-methylenedioxyamphetamine (MDA) in human plasma, sweat, oral
-
Metabolic profile of Phellodendron amurense Rupr. in vivo of rat and its metabolomic study on intervention in rheumatoid arthritis J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-27 Ying Hao, Bingcheng Wang, Yuan Feng, Xiaodong Xin, Yawei Deng, Mingsong Liu, Chunhua Li, Shiqiao Liu, Qingqing Zhang
To analyze the metabolites (blood, urine and feces) in normal rats after intragastric administration of the decoction of Phellodendri Amurensis Cortex (PAC) and to map the metabolic profile of PAC of rat; meanwhile, to evaluate the anti-rheumatoid arthritis (RA) effect of PAC by blood metabolomics technique and to explore its mechanism. Performing on UPLC-Q-TOF-MS technology with a Waters ACQUITY UPLC
-
Pteridine and tryptophan pathways in children with type 1 diabetes: Isoxanthopterin as an indicator of endothelial dysfunction J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-27 Yavuz Özer, Mehmet Şerif Cansever, Hande Turan, Elvan Bayramoğlu, Dilek Bingöl Aydın, Esra İşat, Emre Ceyhun, Tanyel Zubarioğlu, Ayşe Çiğdem Aktuğlu Zeybek, Ertuğrul Kıykım, Olcay Evliyaoğlu
Type 1 diabetes (T1D) and its complications are known to be associated with oxidative stress. Pteridine derivatives and indoleamine 2,3-dioxygenase (IDO) activity can be used as biomarkers in the evaluation of oxidative stress. In this study, our aim is to compare the concentrations of serum and urinary pteridine derivatives, as well as serum IDO activity, in children and adolescents diagnosed with
-
Establishment of a multi-strategy platform for quality control and quality markers screen of Mailuoshutong pill J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-23 Yaojuan Chu, Xiangyu Zhang, Lihua Zuo, Xiaobao Wang, Yingying Shi, Liwei Liu, Lin Zhou, Jian Kang, Bing Li, Wenbo Cheng, Shuzhang Du, Zhi Sun
Thromboangiitis obliterans (TAO) is a non-atherosclerotic segmental inflammatory occlusive disease with a high recurrence rate, high disability rate, difficulty to cure, and poor prognosis. It has been clinically proven that Mailuoshutong pill (MLSTP) is an effective traditional Chinese medicine for treating TAO. As MLSTP contains hundreds of chemical components, the quality control of which is a challenge
-
A GC×GC-MS method based on solid-state modulator for non-targeted metabolomics: Comparison with traditional GC-MS method J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-23 Yueyi Zhang, Haihui Ren, Xiao Tang, Qiaorong Liu, Wen Xiao, Zunjian Zhang, Yuan Tian
The formidable challenge posed by the presence of extremely high amounts of compounds and large differences in concentrations in plasma significantly complicates non-targeted metabolomics analyses. In this study, a comprehensive two-dimensional gas chromatography-quadrupole mass spectrometry (GC×GC-qMS) method with a solid-state modulator (SSM) for non-targeted metabolomics in beagle plasma was first
-
Pharmacokinetic and tissue distribution analysis of bioactive compounds from Fuke Qianjin capsules in rats by a validated UPLC[sbnd]MS/MS method J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-23 Xiujie Guo, Jiaying Yang, Wei Wang, Yun Gong, Peng Zhang, Mengyao Wu, Yuanqing Zheng, Chaoran Wang
Fuke Qianjin capsules (FKQJ) exhibit obvious advantages and characteristics in the treatment of pelvic inflammatory disease. At present, information regarding the in vivo process of FKQJ is lacking, which has become a bottleneck in further determining the therapeutic effect of this traditional Chinese medicine. In the present study, a sensitive, simple and reliable method was developed and validated
-
LC-MS/MS determination of atropine toxicity: Pre-analytical effect of blood collection tube and analytical matrix J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-23 Faten Farouk, Ehab Elkady
Atropine (ATR) intoxication is a recurrent case in emergency departments. The diagnosis is dependent on clinical evaluation and is supported by analytical assessment The assay is limited by the rapid degradation/metabolism of ATR into TRP as well as the preanalytical factors impairing correct detection and diagnosis.
-
Application of electron paramagnetic resonance spectroscopy to examine free radicals formed in indapamide and torasemide storage under UV irradiation and at the higher temperatures which appear under light exposition J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-22 Kinga Orlińska, Paweł Ramos, Katarzyna Komosińska-Vassev, Krystyna Olczyk, Jerzy Stojko, Anna Rzepecka-Stojko, Yoana Kiselova-Kaneva, Diana Ivanova, Paweł Olczyk, Barbara Pilawa
Free radical formation in two diuretics: indapamide and torasemide was examined during UV irradiation and storage at higher temperatures using X-band (9.3 GHz) electron paramagnetic resonance spectroscopy (EPR). The aim of this study was to investigate the possibility of storing indapamide and torasemide under UV irradiation and at higher temperatures, which may occur during exposure to light. The
-
Exploration of the intestinal flora to reveal the important contribution of Radix Astragali to Huangqi Jianzhong Tang in treating chronic atrophic gastritis rats J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-22 Ruonan Zhang, Xuemei Qin, Yuetao Liu
Radix Astragali (Huangqi in Chinese, HQ) is a commonly used Chinese herbal medicine for thousands of years. In this study, A classic prescription Huangqi Jianzhong tang (HQJZ) was selected to evaluate the important effect of HQ on rats with chronic atrophic gastritis (CAG) from the perspective of intestinal flora in cecal contents samples. Traditional pharmacological indicators, including weight change
-
Vortex-blending matrix solid-phase dispersion and UPLC-Q-TOF/MS were proposed to extract and examine the urushiols from Toxicodendron vernicifluum bark J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-22 HongXia Chen, Hao Zhou, Zhiwen Qi, Xingying Xue, ChengZhang Wang
bark has been used for many years as a component in foods and as a traditional herbal medication. Unfortunately, the presence of urushiols, which induce allergies, limits its application. This study used a vortex-blending matrix solid-phase dispersion microextraction technique to extract urushiols from bark. HPLC was used to evaluate the amounts of the extracted urushiols (15:0, 15:1, 15:2, and 15:3)
-
Fingerprick volumetric absorptive microsampling for therapeutic drug monitoring of antiseizure medications: Reliability and real-life feasibility in epilepsy patients J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-21 Chiara Cancellerini, Laura Maria Beatrice Belotti, Susan Mohamed, Martina Solda', Erika Esposito, Francesca Bisulli, Barbara Mostacci, Luca Vignatelli, Paolo Tinuper, Manuela Contin, Laura Licchetta, Tele-Epic study group: Elisa Baldin Raschi Emanuel Marina Trivisano Lidia Di Vito Lorenzo Ferri Cristina Filosomi Nicola Specchio Federico Vigevano Eleonora Matteo
Volumetric absorptive microsampling (VAMS) is increasingly proposed as a clinically reliable therapeutic drug monitoring (TDM) sampling methodology. The study aimed to establish the reliability and real-life feasibility of patient self-collected capillary VAMS for TDM of antiseizure medication (ASMs), using plasma ASMs concentrations from venous blood as a reference standard. Nurses collected venous
-
Deciphering the Molecular Mechanism of Bu Yang Huan Wu Decoction in Interference with Diabetic Pulmonary Fibrosis via Regulating Oxidative Stress and Lipid Metabolism Disorder J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-20 Junfeng Guo, Yuwei Zhang, Rui Zhou, Yanwei Hao, Xuanyu Wu, Ganggang Li, Quanyu Du
Diabetes mellitus type 2 and pulmonary fibrosis have been found to be closely related in clinical practice. Diabetic pulmonary fibrosis (DPF) is a complication of diabetes mellitus, but its treatment has yet to be thoroughly investigated. Bu Yang Huan Wu Decoction (BYHWD) is a well-known traditional Chinese prescription that has shown great efficacy in treating pulmonary fibrosis with hypoglycemic
-
Integrated UHPLC-MS untargeted metabolomics and gut microbe metabolism pathway-targeted metabolomics to reveal the prevention mechanism of Gushudan on kidney-yang-deficiency-syndrome rats J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-20 Ling Xin, Mengxin Ren, Yanwei Lou, Huawen Yin, Feng Qin, Zhili Xiong
Gushudan (GSD) was a traditional Chinese prescription with the remarkable effect of kidney-tonifying and bone-strengthening. However, the potential prevention mechanisms of the GSD on kidney-yang-deficiency-syndrome (KYDS) and its regulation on gut microbe metabolism still need to be further systematically investigated. This study established untargeted urinary metabolomics based on RP/HILIC-UHPLC-Q-Orbitrap
-
Metabolomic analysis reveals the biological characteristics of giant congenital melanocytic nevi J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-18 Yajie Chang, Tao Dai, Ge Song, Sanxi Wang, Huile Pei, Guiping Shen, Jianghua Feng
Giant congenital melanocytic nevi (GCMN) is a congenital cutaneous developmental deformity tumor that usually occurs at birth or in the first few weeks after birth, but its pathogenesis is still unclear. In this study, nuclear magnetic resonance-based metabolomics strategy was employed to evaluate the metabolic variations in serum and urine of the GCMN patients in order to understand its underlying
-
Revealing the anti-inflammatory ingredients in wine-processed Radix et Rhizoma Rhei using immobilized cysteinyl leukotriene receptor type 1 as the stationary phase J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-17 Xuechao Li, Wen Yang, Yu Li, Jing Kang, Jing Wang, Minfeng Fang
Despite the tremendous progress of wine-processed (Jiudahuang, JDH) in removing toxic heat from the blood in the upper portion of the body for hundreds of years, the deep understanding of its functional material basis of the anti-inflammatory ingredients remains unclear due to the lack of high specific and efficient methods. Herein, taking Cysteinyl leukotriene receptor type 1(CysLTR) as the target
-
Validation and application of a method for the quantification of 137 drugs of abuse and new psychoactive substances in hair J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-17 Rossella Barone, Guido Pelletti, Arianna Giorgetti, Susan Mohamed, Jennifer Paola Pascali, Sara Sablone, Francesco Introna, Susi Pelotti
In the dynamic universe of new psychoactive substances (NPS), the identification of multiple and chemically diverse compounds remains a challenge for forensic laboratories. Since hair analysis represents a gold-standard to assess the prevalence of NPS, which are commonly detected together with classical drugs of abuse (DoA), our study aimed at developing a wide-screen method to detect and quantify
-
Screening and evaluation of quality markers of Radix Cudramiae for liver disease based on an integrated strategy of in vivo pharmacokinetics and in vitro HPLC fingerprint J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-17 Qing Liu, Luyao Liu, Lintong Xie, Linyu Zheng, Qianwei Xu, Weidong Li, Xiao Liu
Radix Cudramiae, the dried root of (Lour.) Kudo et Masam., is a valuable ethnomedicine with outstanding antihepatitis activity. However, the lack of reports on quality markers (Q-markers) hindered its quality evaluation and standardization, as a result restricted its clinical application. This paper aimed to discover the Q-markers of Radix Cudramiae with a comprehensive strategy based on pharmacokinetics
-
-
-
Pharmacodynamics and mechanism of Erigeron breviscapus granules in the treatment of ischemic stroke in mice by regulating sphingolipid metabolism based on metabolomics J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-17 Chengzhao Liu, Kedi Liu, Xingru Tao, Weihong Li, Shi Zhao, Qi Dai, Hong Yao, Fei Mu, Sha Chen, Jing Li, Taiwei Dong, Jialin Duan, Peifeng Wei, Feng Gao, Miaomiao Xi
(Vant.) Hand.-Mazz. (EB) granules is the extract preparation of EB, with clear curative effect and unclear mechanism. This study intends to systematically explore the specific mechanism of EB granules in the treatment of IS from the metabolic perspective. The model of transient middle cerebral artery occlusion (tMCAO) in mice was established by the suture-occluded method. The therapeutic effect of
-
Network pharmacology combined with lipidomics to reveal the regulatory effects and mechanisms of Kangzao granules in the hypothalamus of rats with central precocious puberty J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-17 Yanhua Jiang, Lixia Yang, Hui Chen, Jiabin Chen, Lingling Yang, Zhao Wang, Xuejing Yuan, Jinjun Shan, Lili Lin, Hui Li, Jin Ye
Central precocious puberty (CPP) is a prevalent endocrine disorder that primarily affects children, specifically females, and is associated with various physical and psychological complications. Although Kangzao granules (KZG) are efficacious in managing CPP, the underlying mechanisms remain unclear. Therefore, this study aimed to elucidate the therapeutic mechanisms of KZG using network pharmacology
-
Identification and quantification techniques of polymorphic forms - A review J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Julian Ticona Chambi, Cinira Fandaruff, Silvia Lucia Cuffini
In the pharmaceutical industry, the unexpected appearance of crystalline forms could impact the therapeutic efficacy of an Active Pharmaceutical Ingredient (API). For quality control, a thorough qualitative and quantitative monitoring of pharmaceutical solid forms is essential to ensure the detection and the quantification of crystalline forms, wither different or with the same chemical composition
-
Paper-based ligand fishing method for rapid screening and real-time capturing of α-glucosidase inhibitors from the Chinese herbs J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Xinlin Chen, Ying Wu, Sifang Wu, Yucheng Gu, Jianguang Luo, Lingyi Kong
Identifying medicinally relevant compounds from natural resources generally involves the tedious work of screening plants for the desired activity before capturing the bioactive molecules from them. In this work, we created a paper-based ligand fishing platform to vastly simplify the discovery process. This paper-based method exploits the enzymatic cascade reaction between -glucosidase (GAA), glucose
-
Simultaneous quantification of 2-aminothiazoline-4-carboxylic acid and 2-aminothiazoline-4-oxoaminoethanoic acid utilizing chemical derivatization followed by liquid chromatography–tandem mass spectrometry J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Tadashi Nishio, Yoko Toukairin, Tomoaki Hoshi, Tomomi Arai, Makoto Nogami
Detecting cyanide compounds in postmortem blood samples is an important matter in forensic science because cyanide is often used as a poison for murder or suicide. However, the direct analysis of cyanide itself has practical limitations because of cyanide’s volatility and short half-life at ambient temperature. Here, we focused on the relatively stable cyanide metabolites 2-aminothiazoline-4-carboxylic
-
A specific chiral HPLC method for lifitegrast and determination of enantiomeric impurity in drug substance, ophthalmic product and stressed samples J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Merve Uğur, Esen Bellur Atici, Sibel A. Ozkan
Lifitegrast is a lymphocyte function-associated antigen-1 (LFA-1) antagonist used to treat the indications and symptoms associated with dry eye disease (DED), one of the most common ocular surface diseases. Lifitegrast has a chiral center, and the -enantiomer (-Lif) is responsible for the therapeutic effects, while the -enantiomer (-Lif) lacks efficacy in the treatment of DED. Lifitegrast ophthalmic
-
Nontargeted metabolomics and enzyme inhibitory and antioxidant activities for chemical and biological characterization of jujube (Ziziphus jujuba) extracts J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Ya-Ling Yu, Yi-Wen Wu, Yan-Hua Jiao, Fang-Ming Liu, Bin Wang, Jun Cao, Li-Hong Ye
The chemical and biologically active characterization of jujube samples (fruits, cores, and leaves) were carried out by the integrated nontargeted metabolomics and bioassay. Firstly, collision cross-section values of active compounds in jujubes were determined by ultrahigh-performance liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry. Then, a multidimensional
-
Simultaneous determination of BGT-002 and its acyl glucuronide metabolite ZM326E-M2 in human plasma by liquid chromatography-tandem mass spectrometry and its application to a pharmacokinetic study J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Xueran Zhu, Shumin Cui, Xinjing Liu, Mei Zhang, Zhifu Xie, Wei Li, Jingya Li, Fajun Nan, Yangming Zhang, Yan Zhan, Xiaoyan Chen
BGT-002, a new type of ATP-citrate lyase inhibitor, is a promising therapeutic for treatment of hypercholesterolemia. After an oral administration of BGT-002 to subjects, it underwent extensive metabolism and an acyl monoglucuronide (ZM326E-M2) on 1- carboxylic acid group was the major circulating metabolite. In this study, an LC-MS/MS method was developed and validated for the simultaneous determination
-
Co-stimulatory pathway competitive assay development using Liquid chromatography–tandem mass spectrometry (LC-MS/MS) J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-15 Abeer K. Malkawi, Leanne Ohlund, Anas M. Abdel Rahman, Lekha Sleno, Mohamed Siaj
T-cells play a significant role in the development of autoimmune diseases. The CD28-B7 costimulatory pathway is crucial for activating T-cells, and blocking this pathway is essential for treating autoimmune diseases. Therapeutic antibodies and fusion proteins that target costimulatory molecules like CD80, CD86, CTLA-4, and CD28 have been developed to explore the costimulation process and as targeted
-
The material basis of bitter constituents in Carbonized Typhae Pollen, based on the integration strategy of constituent analysis, taste sensing system and molecular docking J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-14 Derong Kong, Ying Zhang, Xinyue Li, Yanyu Dong, Zhiying Dou, Zhen Yang, Mixia Zhang, Hui Wang
The discovery of bitter constituents is of great significance to the exploration of medicinal substances for they have potential physiological effects. Carbonized Typhae Pollen (CTP), which is a typical example of carbonized Traditional Chinese Medicine (TCM), has a bitter taste and hemostatic effect after carbonized processing. The objective of this study is to elucidate the material basis of bitter
-
A Robust TabNet-Based Multi-Classification Algorithm for Infrared Spectral Data of Chinese Herbal Medicine with High-Dimensional Small Samples J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-14 Yongjun Wang, Chengliang Jin, Li Ma, Xiao Liu
Robust classification algorithms for high-dimensional, small-sample datasets are valuable in practical applications. Faced with the infrared spectroscopic dataset with 568 samples and 3448 wavelengths (features) to identify the origins of Chinese medicinal materials, this paper proposed a novel embedded multiclassification algorithm, ITabNet, derived from the framework of TabNet. Firstly, a refined
-
Production of antibody and development of enzyme-linked immunosorbent assay for therapeutic drug monitoring of eravacycline J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-13 Inna A. Galvidis, Anastasia G. Moshcheva, Yury A. Surovoy, Pavel D. Sobolev, Vitaly R. Sharipov, Nikita G. Sidorov, Sergei V. Tsarenko, Maksim A. Burkin
Eravacycline (ERC) was approved for clinical use in 2018. It is more potent than other tetracyclines and can overcome resistance, making it an attractive option for combating multidrug-resistant bacterial infections. Intensive pharmacokinetic (PK) studies are currently being conducted to ensure the effectiveness and safety of ERC in various groups of patients, including those undergoing extracorporeal
-
Simultaneous quantification of four hormone therapy drugs by LC-MS/MS: Clinical applications in breast cancer patients J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-12 Bochra Mansour, Clarice Ngo, Dimitri Schlemmer, Pascal Robidou, Juliette Blondel, Clémence Marin, Gaëlle Noé, Adrien Procureur, Mathieu Jamelot, Joseph Gligorov, Joe-Elie Salem, Noël Zahr
Aromatase inhibitors such as anastrozole, letrozole, exemestane and selective estrogen down-regulator (SERD) fulvestrant are used mostly to treat breast cancer estrogen receptor positive in post-menopausal women. These drugs are given either through the oral route or by intramuscular injection. They have shown great inter-individual variability with a risk of cardiometabolic disorders. Hence the importance
-
Development of simultaneous quantitation method for 20 free advanced glycation end products using UPLC–MS/MS and clinical application in kidney injury J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-12 Yuzhu Xu, Menghe Huang, Yingting Chen, Lintao Yu, Meiran Wu, Shiyue Kang, Qiuyu Lin, Qiaoxuan Zhang, Liqiao Han, Haibiao Lin, Peifeng Ke, Wenjin Fu, Qizhi Tang, Jun Yan, Xianzhang Huang
Advanced glycation end products (AGEs), derived from the non-enzymatic glycation reaction, are defined as glycotoxins in various diseases including aging, diabetes and kidney injury. Exploring AGEs as potential biomarkers for these diseases holds paramount significance. Nevertheless, the high chemical structural similarity and great heterogeneity among AGEs present a formidable challenge when it comes
-
Quantification of oxaliplatin- and ioversol-related compounds in pharmaceutical formulations using novel HPLC-ICP-MS methods J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-10 Qiping Wu, Jianmei Lv, Feifan Xie, Sanwang Li
Accurate quantifying of drug-related compounds in medicines is vital for safety. Commonly used structure-dependent methods rely on analytical standards. High-performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) offers a promising solution, being structure-independent and not requiring standards. In this study, we aim to develop HPLC-ICP-MS methods
-
Qualitative and quantitative analysis of glutathione and related impurities in pharmaceuticals by qNMR J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-10 Qin Shu, Mary Schleiff, Cynthia Sommers, Jingyue Yang, Xiaohui Shen, Jason D. Rodriguez, David Keire
In this study, an alternative method to compendial analytical procedures with enhanced detection and separation capabilities was validated for the quality assessment of glutathione (GSH) drug substance. The related impurities A, B, C, and D present in GSH drug substance were characterized using a one-dimension proton nuclear magnetic resonance (1D H NMR) method on a 600 MHz spectrometer equipped with
-
Mass spectrometry imaging-based metabolomics highlights spatial metabolic alterations in three types of liver injuries J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-10 Zihan Zhu, Yun Zhang, Jun Li, Yuhao Han, Lei Wang, Yaqi Zhang, Haoyuan Geng, Yurong Zheng, Xiao Wang, Chenglong Sun, Baoguo Li, Panpan Chen
Liver's distinctive function renders it highly susceptible to diverse damage sources. Characterizing the metabolic profiles and spatial signatures in different liver injuries is imperative for early diagnosis and etiology-oriented treatment. In this comparative study, we conducted whole-body spatial metabolomics on zebrafish with liver injury induced by ethanol (EtOH), acetaminophen (APAP), and thioacetamide
-
Fentogram electrochemical detection of HIV RNA based on graphene quantum dots and gold nanoparticles J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-09 Anna C.R. Moço, José A.L. Gomide, Jose M.R. Flauzino, Jéssica G. Brussasco, Luiz F.G. Luz, Márcia M.C.N. Soares, João M. Madurro, Ana G. Brito-Madurro
This work reports the construction of an HIV-specific genosensor through the modification of carbon screen-printed electrodes (CSPE) with graphene quantum dots decorated with L-cysteine and gold nanoparticles (cys-GQDs/AuNps). Cys-GQDs were characterized by FT-IR and UV–vis spectra and electronic properties of the modified electrodes were evaluated by cyclic voltammetry and electrochemical impedance
-
Impacts of small-molecule STAT3 inhibitor SC-43 on toxicity, global proteomics and metabolomics of HepG2 cells J. Pharmaceut. Biomed. Anal. (IF 3.4) Pub Date : 2024-02-09 Ting Mao, Peikun He, Zhichao Xu, Yingying Lai, Jinlian Huang, Zhijian Yu, Peiyu Li, Xianqiong Gong
In this study, we aimed to investigate the cytotoxicity and potential mechanisms of SC-43 by analyzing the global proteomics and metabolomics of HepG2 cells exposed to SC-43. The effect of SC-43 on cell viability was evaluated through CCK-8 assay. Proteomics and metabolomics studies were performed on HepG2 cells exposed to SC-43, and the functions of differentially expressed proteins and metabolites