-
Integrated network pharmacology, molecular docking, and lipidomics to reveal the regulatory effect of Qingxuan Zhike granules on lipid metabolism in lipopolysaccharide‐induced acute lung injury Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-16 Hui Chen, Jiabin Chen, Lu Feng, Hua Shao, Yang Zhou, Jinjun Shan, Lili Lin, Jin Ye, Shouchuan Wang
Qingxuan Zhike granules (QXZKG), a traditional Chinese patent medication, has shown therapeutic potential against acute lung injury (ALI). However, the precise mechanism underlying its lung‐protective effects requires further investigation. In this study, integrated network pharmacology, molecular docking, and lipidomics were used to elucidate QXZKG's regulatory effect on lipid metabolism in lipop
-
A novel strategy for the quality control of carbonized Typhae pollen using colorimeter, liquid chromatography–mass spectrometry, and efficacy evaluation coupled with multivariate statistical analysis Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-15 Li Jiaqi, Tang Min, Zhong Yongqi, Li Xiaolong, Ge Yuewei, Wang Shumei, Liang Shengwang, Meng Jiang, Sun Fei
In this study, a novel quality control strategy was proposed, aiming to establish a multivariate specification for the processing step by exploring the correlation between colors, chemical components, and hemostatic effects of the carbonized Typhae pollen (CTP) using multivariate statistical analysis. The CTP samples were stir‐fried at different durations. Afterward, the colorimeter and LC–MS techniques
-
Issue Information Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-14
No abstract is available for this article.
-
Protective effect of Xixin–Ganjiang herb pair for warming the lungs to dissolve phlegm in chronic obstructive pulmonary disease rats based on integrated network pharmacology and metabolomics Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-07 Ping Huang, Ting Xiang, Qiong Wang, Lintao Han, Sili Zheng, Dongning Zhang, Fang Huang, Bailu Duan, Jingjing Li, Huamao Li, Tao Huang
Xixin–Ganjiang herb pair (XGHP) is a classic combination for warming the lungs to dissolve phlegm and is often used to treat a variety of chronic lung diseases; it can treat the syndrome of cold phlegm obstruction of lungs. First, ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) was used to examine the composition of XGHP, and network pharmacology was used to predict its
-
Pharmacokinetic study of multicomponent in Hong‐Hua‐Xiao‐Yao tablet Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-06 Xiaofeng Li, Leyi Huang, Junping Xiao, Xuemei Zhang
Hong‐Hua‐Xiao‐Yao tablet (HHXYT) is attracting attention increasingly because of its use in treatment of mammary gland hyperplasia (MGH) and menopausal syndrome. However, its pharmacokinetics remains unclear. This study developed a sensitive and rapid method for simultaneous determination of 10 compounds of HHXYT in rat plasma by liquid chromatography–tandem mass spectrometry and to compare the pharmacokinetics
-
Untargeted metabolomics reveals potential plasma biomarkers for diagnosis of primary aldosteronism using liquid chromatography–mass spectrometry Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-05 Jing‐Jing Song, Jun Cai, Wen‐Jun Ma, Ying Lou, Jin Bian, Beibei Zhao, Xuhui She, Xiao‐Ning Liu
Metabolite profiling has the potential to comprehensively bridge phenotypes and complex heterogeneous physiological and pathological states. We performed a metabolomics study using parallel liquid chromatography–mass spectrometry (LC–MS) combined with multivariate data analysis to screen for biomarkers of primary aldosteronism (PA) from a cohort of 111 PA patients and 218 primary hypertension (PH)
-
LC–MS/MS determination of N‐acetyl‐l‐cysteine in chicken plasma Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-03-04 Albena Roydeva, Aneliya Milanova
N‐acetyl‐l‐cysteine (NAC) shows beneficial effects in cases of aflatoxicosis and heat stress in poultry but little is known about its pharmacokinetics in chickens. Therefore, the study aimed to develop and validate a sensitive LC–MS/MS analytical method for quantitative analysis of NAC in chicken plasma. A split calibration curve approach was used for determination of NAC in chicken plasma. Standard
-
In vivo and in vitro metabolite profiling of nirmatrelvir using LC–Q-ToF–MS/MS along with the in silico approaches for prediction of metabolites and their toxicity Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-25 Sowmya Chaganti, Bhoopendra Singh Kushwah, Laximan Velip, Shristy S. Tiwari, Shrilekha Chilvery, Chandraiah Godugu, Gananadhamu Samanthula
Nirmatrelvir (NRV), a 3C-like protease or Mpro inhibitor of SARS-CoV-2, is used for the treatment of COVID-19 in adult and paediatric patients. The present study was accomplished to investigate the comprehensive metabolic fate of NRV using in vitro and in vivo models. The in vitro models used for the study were microsomes (human liver microsomes, rat liver microsomes, mouse liver microsomes) and S9
-
Proteomic analysis of exosomal proteins associated with bone healing speed in a rat tibial fracture model Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-27 Haifeng Hong, Changhui Lin, Min Fang, Juntian Liu, Hung‐Chih Hsu, Chih‐Jung Chang, Haibing Wang
This study investigates the impact of exosomes on bone fracture healing in a rat tibial model, distinguishing between fast and slow healing processes. Bone healing and protein expression were assessed through X‐ray examinations, hematoxylin and eosin staining, and immunohistochemical staining. Exosomes were isolated, characterized and subjected to liquid chromatography–mass spectrometry for protein
-
Bioavailability assessment of a brompheniramine taste‐masked pediatric formulation in a juvenile porcine model Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-27 Jiang Wang, Diaa Shakleya, George Giacoia, Ziyuar Rahman, Mansoor A. Khan, Patrick J. Faustino
A brompheniramine taste‐masked pediatric formulation was developed as part of the National Institutes of Health Pediatric Formulation Initiative to help address low patient compliance caused by the bitter taste of many adult formulations. To confirm that the taste‐masked formulation can provide a similar pharmacological effect to the previous marketed adult formulations, a juvenile porcine model was
-
Integrating network pharmacology and renal metabonomics to reveal the protective mechanism of resveratrol on gouty nephropathy Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-25 Xiaomin Xu, Donghua Yu, Yu Wang, Peng Xu, Xin Jiang, Fang Lu, Shumin Liu
Resveratrol (Res) has been demonstrated to have beneficial effects on gouty nephropathy (GN). However, the mechanisms of Res on GN remain unclear. This study aimed to investigate the mechanisms of Res on GN. In this study, network pharmacology technology was used to predict the Res targets in the prevention and treatment of GN. Renal metabonomics was used to identify differential metabolites in kidney
-
Screening of polysaccharides from the differently processed products of Angelica sinensis with the best liver protection effect on chicken and the intervention mechanism study based on tandem mass tag proteomics and multiple reaction monitoring approach Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-25 Chen‐chen Li, Peng Ji, Jian He, You‐sheng Peng, Fan‐lin Wu, Yong‐li Hua, Wan‐ling Yao, Zi‐wen Yuan, Yan‐ming Wei
The incidence of colibacillosis in poultry is on the rise, significantly affecting the chicken industry. Ceftiofur sodium (CS) is frequently employed to treat this disease, resulting in lipopolysaccharide (LPS) buildup. Processing plays a vital role in traditional Chinese veterinary medicine. The potential intervention in liver injury by polysaccharides from the differently processed products of Angelica
-
Issue Information Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-22
No abstract is available for this article.
-
Analytical determination of ethylenediamine impurity in tripelennamine hydrochloride by gas chromatography–mass spectrometry using phthalaldehyde as the derivatizing agent Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-22 Sayyad Kousrali, Leela Prasad Kowtharapu, Tanmoy Mondal
In the pharmaceutical industry, effective risk management and control strategies for potential genotoxic impurities are of paramount importance. The current study utilized GC–MS to evaluate a precise, linear, and accurate analytical method for quantifying ethylenediamine present in tripelennamine hydrochloride using phthalaldehyde as a derivatizing agent. When phthalaldehyde is sonicated for 10 min
-
Chromatographic authentication of botanical origin: Herbaceous pollen profiling with HPLC, HPTLC and GC–MS analysis Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-21 Nozimova Aziza, Khislat Khaydarov, Muhammad Zafar, Waleed A. A. Alsakkaf, Jawaher Alkahtani, Mushtaq Ahmad, Trobjon Makhkamov, Zamira Djumayeva, Gokhan Zengin, Tursunboev Khamdam Eshboyevich, Aferin Beilerli, Ilgiz Gareev, Ulugbek Ochilov, Islamov Boston Sultanovich, Umurzakova Zebiniso Iskandarovna, I Putu Agus Hendra Wibawa
This study describes a robust chromatographic authentication methodology for herbaceous pollen, employing gas chromatography–mass spectrometry (GC–MS), high‐performance liquid chromatography (HPLC) and high‐performance thin liquid chromatography (HPTLC) protocols. The comprehensive profiling of organic compounds not only distinguishes between different botanical sources but also establishes a reliable
-
Spectrum–effect relationship study between ultra-high-performance liquid chromatography fingerprints and anti-hepatoma effect in vitro of Cnidii Fructus Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-18 Zhenguo Lv, Huizi Ouyang, Fanjiao Zuo, Minglei Ge, Mengxuan Wu, Lulu Zhao, Yameng Zhu, Xinxin Miao, Yu Bai, Yanxu Chang, Jun He
Cnidii Fructus, derived from the dried ripe fruit of Cnidium monnieri (L.) Cuss, has the effect of warming kidneys and invigorating Yang. This study established the spectrum–effect relationships between ultra-high-performance liquid chromatography (UHPLC) fingerprints and the antitumor activities of Cnidii Fructus on human hepatocellular carcinoma (HepG2) cells. In UHPLC fingerprints, 19 common peaks
-
Investigation of the therapeutic effect of Hedan tablets on high-fat diet-induced obesity in rats by GC–MS technology and 16S ribosomal RNA gene sequencing Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-18 Xuan Pan, Yumeng Zhang, Yongyao Qiao, Qingying Cao, Liuxin Wei, Min Zhao
Obesity is a persistent metabolic condition resulting from the excessive accumulation or abnormal distribution of body fat. This study aimed to establish an experimental rat model of obesity. The efficacy of treating obesity with Hedan tablets (HDT) was assessed by monitoring changes in weight, blood lipid levels, analyzing inflammatory factors, evaluating organ indices, and observing liver tissue
-
Identifying chemical markers in wine-processed Salvia miltiorrhiza using ultrahigh-performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-14 Tingting Xu, Xiuwei Li, Guodong Sun, Wenfeng Wei, Jinhai Huo, Weiming Wang
To find the chemical markers of wine-processed Salvia miltiorrhiza (WSM), 76 constituents, including diterpenoid quinones and phenolic acids in Salvia miltiorrhiza (SM) and WSM, were profiled using ultrahigh-performance liquid chromatography-quadrupole-time-of-flight-tandem mass spectrometry (UPLC-Q-TOF-MS/MS) in positive- and the negative-ion modes. Thirty compounds were screened out as candidate
-
Chemical profile and difference analysis of four parts of Strobilanthes sarcorrhiza by ultrafast flow liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry and multivariate statistical analysis Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-11 Zheng Tian, Yu Zhang, Xin-zhi Wang, Xiao-bing Cui, Wei Li, Xian-he Tan, Jia-jia Chen, Hai-zhong Lin, Hong-mei Wen
Strobilanthes sarcorrhiza (CTS) is a medicinal plant with various pharmacological effects such as tonifying kidney and anti-inflammatory. However, the chemical composition and difference of its four parts (leaves, stems, rhizomes, and root tubers) have been rarely reported. In this study, ultrafast flow liquid chromatography coupled with quadrupole-time-of-flight MS was applied to analyze the chemical
-
LC–MS/MS method for the quantification of cortisol of hepatocellular carcinoma Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-13 Zijing Zhou, Xiaoshuang Li, Shengjun Yin, Zhiyuan Zhang, Xiaoyue Li, Xiaojie Miao, Haixu Cheng, Yaqing Lou, Pengfei Tu, Yingyuan Lu, Guoliang Zhang
The imbalance of steroid hormones is closely related to the occurrence and development of hepatocellular carcinoma (HCC). However, most research has focused on steroid hormone receptors, and reports about the relationship between the serum concentration of cortisol and the development of HCC are rare. The aim of this research was to establish a simple, specific, sensitive and reliable liquid chromatography–mass
-
Determination of three C18-oxygenated steroids in adrenal lesion segments in primary aldosteronism by super-selective adrenal venous sampling and LC/ESI–MS/MS Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-07 Yuka Mizumoto, Ayaka Hirakawa, Yuki Sugiura, Tetsuo Nishikawa, Koshiro Nishimoto, Yasunari Mano, Tatsuya Higashi
Super-selective adrenal venous sampling (ssAVS) can collect the adrenal tributary venous blood in the aldosterone (ALD)-hypersecreting segments in primary aldosteronism. The concentrations of the C18-oxygenated steroids, especially 18-oxocortisol (18-oxoF), in the lesion segments might be more useful indices than those in the peripheral or adrenal central veins (current candidate indexes) for the differential
-
LC–HRMS methods for the quantification of two artemisinin drugs and their metabolites in rat blood and plasma Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-07 Hongchang Zhou, Huixiu Mao, Jie Xing
As first-line antimalarials used in the artemisinin combination therapy, artemisinin drugs exert their action inside red blood cells. However, the blood pharmacokinetic characteristics of artemisinin drugs have not been fully revealed owing to their built-in chemical instability initiated by Fe2+ released from hemoglobin, with limited information on their metabolites. In this study, liquid chromatography
-
M2 tumor–associated macrophages and CXCL2 induce lipid remodeling in hepatocellular carcinoma cell lines Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-05 Yongling Chen, Ziqing Zhong, Yan Deng, Yu Lu, Xue Qin
Primary hepatocellular carcinoma (HCC) is one of the most common malignant tumors, but its pathogenesis remains incompletely elucidated. Recently, many studies indicated that lipid remodeling plays an important role in the occurrence and development of HCC. Furthermore, lipids have been proven to be indispensable mediators in promoting communication between tumor cells and extracellular matrix in the
-
Pharmacokinetics, tissue distribution, and excretion study of GL-V9 and its glucuronide metabolite 5-O-glucuronide GL-V9 in Sprague–Dawley rats Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-06 Xuefeng Zhang, Guanlan Liu, Zechun Sang, Xiaoxin Jin, Yan Wang, Qinglong Guo, Yuxin Zhou, Xiuming Song
The objective of this study is to explore the pharmacokinetics, tissue distribution, and excretion patterns of GL-V9 and its glucuronide metabolite, 5-O-glucuronide GL-V9, following the administration of GL-V9 to Sprague–Dawley (SD) rats. In this research, we developed and validated rapid, sensitive, and selective ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) methods
-
A comprehensive quality evaluation strategy of Shensong Yangxin capsules based on qualitative, fingerprint and quantitative analyses Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-05 Zixuan Fu, Yumeng Zhang, Tong Jin, Zheyong Wang, Chunjie Zhao, Min Zhao
Shensong Yangxin capsule (SSYXC), an effective Chinese patent medicine, has been recorded in the Chinese Pharmacopeia, mainly for the treatment of coronary heart disease and ventricular premature beat. To further complete the quality evaluation of SSYXC, a comprehensive analysis strategy was established. Firstly, the components of SSYXC were qualitatively analysed using ultra-high- performance liquid
-
Incurred sample reanalysis (ISR) in clinical bioanalysis—Is it really a one-size-fits-all solution? Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-05 Nuggehally R. Srinivas, Michael G. Bartlett
CONFLICT OF INTEREST STATEMENT The authors declare that they have no conflicts of interest to report.
-
Nontargeted metabolomics reveals sequential changes in amino acid and ferroptosis-related metabolism in Parkinson's disease Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-02 Delai Xu, Jing Dai, Liuxing Tang, Jie Pan, Hua Zhang
Parkinson's disease (PD) is inseparable from metabolic disorders but lacks assessment of specific metabolite alteration. To explore the sequential metabolic changes in PD progression, we evenly divided 78 C57BL/6 mice (10 weeks) into six groups (one control group and five experimental groups) and collected the hippocampus tissue of mice after treating with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
-
Assessment of flusilazole residues on apples: Detection, quantification and health risk implications Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-02-02 Zakir Amin, Parveez Ahmad Sheikh, Ishrat Jan, Baby Summuna, Fayaz Ahmad Mohiddin
Apple, a major fruit of temperate Himalayas, is sprayed with chemical pesticides around 12 times during the cropping season. Various systemic and contact fungicides are applied for the management of major diseases. In order to manage disease, flusilazole 40 EC is frequently used. However, excessive chemical application has been found to be detrimental for consumer safety. Keeping in view consumer safety
-
A simple and sensitive liquid chromatography triple quadrupole mass spectrometry method for the determination of XL092 in monkey plasma and its application to pharmacokinetic study Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-30 Cui Li, Xiaokun Li, Ali Fan, Ning He, Dongmei Wu, Hongyan Yu, Kun Wang, Weijie Jiao, Xu Zhao
XL092 is a potent ATP-competitive inhibitor of multiple receptor tyrosine kinases that is undergoing clinical development for the treatment of lung cancer. In this study, an LC triple quadrupole mass spectrometry method was established to measure XL092 in monkey plasma. A Waters ACQUITY UPLC BEH C18 column was used for chromatographic separation. The mobile phase consisted of water containing 0.1%
-
Mycotoxin detection in selected medicinal plants using chromatographic techniques Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-30 Ihsan ul Haq, Raheela Taj, Muhammad Nafees, Arshad Hussain
Mycotoxins are toxic mycological products that when consumed, absorbed or inhaled cause sickness or even the death of humans. Therefore, the present study aimed to evaluate the contamination levels of mycotoxins (aflatoxins, AFB1, AFB2, AFG1, AFG2, and ochratoxin A, OTA) in selected medicinal herbs and shrubs using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). A
-
Effects of vitamin C on the pharmacokinetics and pharmacodynamics of nimodipine in rats Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-29 Xian-xin Meng, Guan-jun Nan, Hao Hu, Jian-yu He, Yun-zhe Li, Guang-de Yang
In recent years, researchers have shown a growing interest in the interactions between different pharmaceutical agents. An intriguing instance lies in the possible interaction between nimodipine and vitamin C. To investigate the pharmacokinetic and pharmacodynamic effects of vitamin C on nimodipine in rats, rats were randomly divided into a nimodipine only group and a combination group (nimodipine + vitamin
-
Issue Information Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-24
No abstract is available for this article.
-
Pharmacokinetics, absolute bioavailability, and tissue distribution of WJ-14, a novel N-methyl-d-aspartate receptor antagonist, in rats by liquid chromatography–tandem mass spectrometry Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-22 Weiyin Huang, Yan Zhang, Lishou Yang, Yiwen Zhang, Lei Cheng, Yan Deng, Xinyu Wu, Tingting Wang, Xiaosheng Yang, Linhu Ye
To circumvent the limitations of current antidepressants, WJ-14, a novel N-methyl-d-aspartate receptor antagonist, was synthesized and demonstrated to have remarkable efficiency in the treatment of depression. To illustrate the pharmacokinetics, absolute bioavailability, and tissue distribution of WJ-14 in rats, a rapid and sensitive liquid chromatography–tandem mass spectrometry–based analytical method
-
Integration of metabolomics and network pharmacology technology to explain the effect mechanisms of Danggui Buxue decoction in vascular dementia Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-18 Qin Fan, Xinhong Liu, Yanying Zhang, Wanrong Kang, Shanshan Si, Hongmei Zhang
Danggui Buxue decoction (DBD) is a traditional Chinese medicine herbal decoction that has a good therapeutic effect on vascular dementia (VaD). However, its pharmacodynamic substances and underlying mechanisms are ambiguous. The work aimed to decipher the pharmacodynamic substances and molecular mechanisms of DBD against VaD rats based on gas chromatography–mass spectrometry metabonomics, network pharmacology
-
Liquid chromatography–tandem mass spectrometry determination of bumetanide in human plasma and application to a clinical pharmacokinetic study Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-17 Ganesan Padmini Tamilarasi, Krishnan Manikandan, Viswas Raja Solomon
Determining a drug's bioavailability and bioequivalence is important for developing and approving a drug product. The procedure supports applications for generic drug products and novel therapeutic substances, makes important decisions regarding safety and efficacy, and measures a drug's concentration in biological matrices. This study aimed to develop and validate a specific, simple, sensitive, and
-
Structure of crude polysaccharides from Atractylodes lancea rhizome and treatment of diarrhea owing to spleen deficiency through intestinal flora Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-17 Yue Wang, Sheng Wang, Tie-Lin Wang, Li-kun Chang, Jun-Hui Zhou, Ke Yang, Lan-Ping Guo
To optimize the extraction process of crude polysaccharides from Atractylodes and elaborate the mechanism of Atractylodes polysaccharides in treating diarrhea owing to spleen deficiency, so as to lay a foundation for further development and utilization of Atractylodes lancea, we used an orthogonal test to optimize the extraction process and established a model of spleen deficiency. It was further combined
-
Development of a novel quality by design–enabled stability-indicating HPLC method and its validation for the quantification of nirmatrelvir in bulk and pharmaceutical dosage forms Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-16 Pallavi Alegete, Saisudha Byreddy
A systematic and novel quality by design–enabled, rapid, simple, and economic stability–indicating HPLC method for quantifying nirmatrelvir (NMT) was successfully developed and validated. An analytical target profile (ATP) was established, and critical analytical attributes (CAAs) were allocated to meet the ATP requirements. The method used chromatographic separation using a Purosphere column with
-
Development of an ultra-high-performance liquid chromatography–tandem mass spectrometry method for the simultaneous determination of crassicauline A, fuziline, karacoline, and songorine in rat plasma and application in their pharmacokinetics Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-13 Fan Chen, Ziyue Wang, Lvqi Luo, Yifan He, Yizhe Ma, Congcong Wen, Xianqin Wang, Xiuwei Shen
In this paper, an ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed for quantifying the levels of crassicauline A, fuziline, karacoline, and songorine in rat plasma. After processing the rat plasma, the proteins in the plasma were separated by extracting the analytes with acetonitrile–methanol (9:1, v/v). The chromatographic column used was the
-
Multimodal integrated strategy for the discovery and identification of antiplatelet aggregation Q-markers in Paris polyphylla var. yunnanensis Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-12 Yang Li, Rouyuan Wen, Wanqing Yang, Huimei Xu, Qiufeng Xie, Le Wang, Hanzhu Sun, Haizhu Zhang, Conglong Xia
To enhance the quality evaluation and control of traditional Chinese medicine (TCM) and ensure the safety and efficacy of clinical medication, it is imperative to establish a comprehensive quality assessment method aligned with TCM efficacy. This study uses a representative Chinese medicine with multi-origin and multi-efficacy, Paris polyphylla var. yunnanensis (PY), as an illustrative example. Surprisingly
-
Insight gained via ultra-performance liquid chromatography tandem mass spectrometry-based metabolomics: Protective effect of Artemisia argyi H.Lév. & Vaniot essential oil on lipopolysaccharide-induced liver injury mice Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-11 Hui Zhou, Ying Rong, Fanglin Liu, Tong Yu, Weiqi Cui, Qianwen Cao, Luyao Liu, Zhaolong Qin, Xiaoge Ma, Nan Zhang, Youcai Tang, Xia Xu
Artemisia argyi H.Lév. & Vaniot essential oil (AAEO) has shown pharmacological effects such as anti-inflammation, antioxidant, and anti-tumor properties. However, the protective effect of AAEO on lipopolysaccharide (LPS)-induced liver injury and its potential protective mechanism are still unclear. In this study, we used ultra-performance liquid chromatography tandem mass spectrometry metabolomics
-
Effect of vinegar steaming on the composition and structure of Schisandra chinensis polysaccharide and its anti-colitis activity Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-08 Zhi-jun Guo, Wei Zhang, Jin-guo Xu, Xiao-man Li, Jiu-ba Zhang, Yu Li, De Ji, Lin Li, Wei Huang, Lian-lin Su
In this study, infrared spectroscopy, high-performance liquid chromatography, and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry (MALDI-TOF-MS) technology were applied to systematically explain the Schisandra chinensis’s polysaccharide transformation in configuration, molecular weight, monosaccharide composition, and anti-ulcerative colitis (UC) activity after vinegar
-
Comparative phytochemical study of methanolic and ethanolic extracts of Thymus linearis and their antibacterial and antioxidant potential Biomed. Chromatogr. (IF 1.8) Pub Date : 2024-01-08 Hafiz Amir Nadeem, Muhammad Pervaiz, Anam Ejaz, Zohaib Saeed, Muhammad Imran, Rana Rashad Mahmood Khan, Umer Younas
Thymus linearis (Thyme) is a medicinal plant widely distributed throughout Asia. Various parts of thyme are utilized for diverse medicinal purposes, including its use as a tonic and diuretic, for cough relief, as a flavoring agent, in treating dysentery, and for alleviating stomach disorders. Numerous studies have been conducted to explore the unexploited potential of thyme. Thyme was collected from
-
Simultaneous and trace-level quantification of four benzene sulfonate potential genotoxic impurities in doxofylline active pharmaceutical ingredients and tablets using high-performance liquid chromatography with ultraviolet detection Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-30 Tingyu Xiao, Huaqing Lin, Jiekeng Lao, Xin Hu, Yucheng Chen, Yicong Lei, Mingzhi Xu
In the production of doxofylline, the common occurrence of toxic p-toluene sulfonate generation prompted the development and validation of a method using HPLC with ultraviolet detection (HPLC-UV). This method is designed for detecting four potential genotoxic impurities (PGIs) present in both doxofylline drug substance and tablets, with a focus on the UV-absorbing group p-toluene sulfonate. The four
-
Development of a simultaneous quantification method of imatinib and sunitinib and their main metabolites and its application in patients with gastrointestinal stromal tumor Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-28 Hefen Chen, Jianbo Lyu, Zhujun Gong, Yong Han, Kaixiong Tao, Hong Zhou
Correlations between plasma concentrations of imatinib and sunitinib with efficacy and toxicity have been established. It is crucial to develop a sensitive and precise method for determining the plasma concentrations of imatinib and sunitinib, along with their active metabolites, to facilitate therapeutic drug monitoring and individualized therapy. Plasma samples were separated on an Agilent ZORBAX
-
Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-28 Letícia Meier, Estevan Bruginski, Joseane Righes Marafiga, Letícia Barbieri Caus, Mayara Vendramin Pasquetti, Maria Elisa Calcagnotto, Francinete Ramos Campos
Temporal lobe epilepsy (TLE) is a common form of refractory epilepsy in adulthood. The metabolic profile of epileptogenesis is still poorly investigated. Elucidation of such a metabolic profile using animal models of epilepsy could help identify new metabolites and pathways involved in the mechanisms of epileptogenesis process. In this study, we evaluated the metabolic profile during the epileptogenesis
-
Optimization and validation of high-performance liquid chromatography using modified QuEChERS to determine anthelmintic drugs in mutton Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-26 Hiwa Muhammad Husein Hiwa, Khulod Ibraheem Hassan Khulod
The aim of this study was to rapidly determine the presence of anthelmintic drugs in sheep meat using the optimized high-performance liquid chromatography-ultraviolet (HPLC-UV) method with modified QuEChERS (quick, easy, cheap, effective, rugged, safe) technology. Fifty fresh sheep meat samples from different slaughterhouses were collected. A double extraction procedure (QuEChERS/HPLC-UV technology)
-
Development of stability-indicating assay method and liquid chromatography-quadrupole-time-of-flight mass spectrometry-based structural characterization of the forced degradation products of alpelisib Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-26 Vidya Ghanghav, Ashwini Chawathe, Siddheshwar Kisan Chauthe, Nitish Sharma
The US Food and Drug Administration and the European Medicines Agency approved alpelisib in 2019 for the treatment of metastatic breast cancer. A thorough literature review revealed that a stability-indicating analytical method (SIAM) is not available for the quantification of alpelisib and its degradation products (DPs). In this study, per the comprehensive stress study recommended by the International
-
Development and validation of a liquid chromatography–tandem mass spectrometry method for the quantification of the acetyl-coenzyme A competitive p300/CBP catalytic inhibitor A-485 in biological samples Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-26 Reinhard Oertel, Nikolai Jaschke, Annemarie Lippert, Bertold Renner
The small molecule A-485 competitively inhibits the histone acetyltransferase domain of CBP (cyclic-adenosine monophosphate response element-binding protein)/p300. Apart from its antineoplastic activity, researchers are exploring its potential benefits in treating osteoporosis and its impact on energy metabolism. However, so far, only limited pharmacokinetic data are available, and the crucial determination
-
Metabolomic study of the estrogenic and anti-osteoporotic potential of Erythrina bidwillii leaf Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-25 Enaam M. AbouZeid, Rehab A. Hussein, Abeer A. Salama, Fadia S. Youssef, Sherweit H. El-Ahmady, Nagwa Mohamed Ammar, Ahmed H. Afifi
Erythrina bidwillii Lindl., Leguminosae, constitutes a valuable crop for horticulture and medicine; however, it is rarely investigated. Menopause is a crucial transitional period in women’s health. Women worldwide consider the use of phytoestrogens as a safe hormone replacement therapy to alleviate detrimental menopausal symptoms. Thus, the discovery of novel phytoestrogens is highly demanded. The
-
Integration of lipidomics and metabolomics reveals plasma and urinary profiles associated with pediatric Mycoplasma pneumoniae infections and its severity Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-22 Hui Chen, Jia-bin Chen, Li-na Du, Hai-xia Yuan, Jin-jun Shan, Shou-chuan Wang, Jin Ye, Li-li Lin
Mycoplasma pneumoniae is a significant contributor to lower respiratory infections in children. However, the lipidomics and metabolics bases of childhood M. pneumoniae infections remain unclear. In this study, lipidomics and metabolomics analyses were conducted using UHPLC-LTQ-Orbitrap XL mass spectrometry and gas chromatography-triple quadrupole mass spectrometry on plasma (n = 65) and urine (n = 65)
-
Design of experiment-oriented development and validation of novel bioanalytical reverse phase high performance liquid chromatography method of palbociclib in rat plasma and tissues, and its application in pharmacokinetic studies Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-21 Naveen Rajana, Yeruva Sri Pooja, Valamla Bhavana, Padakanti Sandeep Chary, Komalatha Dukka, Rajeshwari Deshmukh, Neelesh Kumar Mehra
The current research involved the development and validation of an easy, cost-effective, and sensitive bioanalytical reverse-phase high-performance liquid chromatography method for the assessment of palbociclib (PAL) in rat plasma and kidney, liver, spleen and heart. A response surface methodology-based Box–Behnken design was used to optimize critical chromatographic conditions such as buffer pH, organic
-
Issue Information Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-20
No abstract is available for this article.
-
A liquid chromatography–tandem mass spectrometry method development for the quantification of favipiravir drug and its related impurities in rat plasma and its application to pharmacokinetic studies Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-21 Kalyani Koganti, Namburi L. A. Amara Babu, Naga Raju Sattu, Koya Prabhakara Rao
Favipiravir is an antiviral drug used for the treatment of virus-based diseases such as influenza. In this context, the development of a reliable liquid chromatography–tandem mass spectrometry method for the quantification of the drug and its impurities is necessary, particularly following the COVID-19 pandemic. Chromatographic separation was achieved on an inertial ODS column using gradient elution
-
Si Miao San relieves hyperuricemia by regulating intestinal flora Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-20 Yue Qian, Yan Shen
This study seeks to investigate the therapeutic effects of Si Miao San (SMS) on hyperuricemia and its underlying mechanisms, particularly focusing on the role of intestinal flora. The key components of SMS were identified using high-performance liquid chromatography (HPLC). To establish a rat model of hyperuricemia, an intraperitoneal injection of potassium oxonate was performed, followed by oral administration
-
Pharmacokinetics, tissue distribution, and plasma protein binding ratio of bicuculline following intragastric and intravenous administration in rats using ultra-high-performance liquid chromatography–tandem mass spectrometry Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-18 Weikang Chen, Yangbin Zheng, Yanyan Zhu, Dehong Liu, Lianghui Zhu
Bicuculline is a natural isoquinoline alkaloid that works as a gamma-aminobutyric acid receptor antagonist. It is widely found in Papaveraceae plants used in traditional Chinese medicines. Bicuculline not only has been shown to have favorable analgesic, memory-improving, and anxiolytic effects but may also cause adverse effects such as convulsions and epilepsy. A simple, rapid, and sensitive method
-
Molecular mechanism of Xiaohuoluo wan for rheumatoid arthritis by integrating in vitro and in vivo chemomics and network pharmacology Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-18 Wanli Qin, Kai Zhang, Ziyang Yu, Heyuan Liu, Hangyu Li, Ling Dong, Dongran Han, Tiangang Li
The cause of rheumatoid arthritis (RA) is unclear. Xiaohuoluo wan (XHLW) is a classical Chinese medicine that is particularly effective in the treatment of RA. Given the chemical composition of XHLW at the overall level has been little studied and the molecular mechanism for the treatment of RA is not clear, we searched for the potential active compounds of XHLW and explored their anti-inflammatory
-
Evaluation of antidiabetic and antioxidant effects of Polygonum cognatum Meisn. and phytochemical analysis of effective extracts Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-18 Sefa Gözcü, Rüstem Anıl Uğan, Hilal Özbek, Betül Gündoğdu, Zühal Güvenalp
Polygonum cognatum Meisn. (Polygonaceae) is used both as food and as a folk medicine to treat diabetes. This study aimed to evaluate the effect of the extracts, along with isolated compounds, from P. cognatum aerial parts on diabetes. In vitro studies were conducted using an α-glucosidase inhibitory assay, while in vivo antidiabetic studies were carried out on streptozotocin-induced diabetic rats.
-
Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-14 Wenhao Cheng, Zheng Yuan, Siyang Wu, Xin Yu, Kexin Xia, Lifeng Zhao, Yuyan Wang, Chen Kang, Wei Yang, Luyang Liu, Yingfei Li
In this present study, we developed a reliable and simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) assay for the simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin and isomaltopaeoniflorin in beagle dog plasma. We also analyzed the pharmacokinetics of those components after oral administration of fried Radix Paeoniae Alba
-
Two-dimensional liquid chromatography measurement of meropenem concentration in synovial fluid of patients with periprosthetic joint infection Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-10 He Jia-Wei, Wang Jing, Cao Li, Zhang Xiao-Gang, Li Guo-Qing, Xu Bo-Yong, Ji Bao-Chao, Huang Jun-jie, Zhao Jun
Periprosthetic joint infection (PJI) is a catastrophic complication following joint replacement surgery. One potential treatment approach for PJI could be the combination of one-stage revision and intra-articular infusion of antibiotics. Meropenem is one of the commonly used intra-articular antibiotics in our institution. Determining the concentration of meropenem in the joint cavity could be crucial
-
Ultra-high-performance liquid chromatography–quadrupole time of flight tandem mass spectrometry based in vitro metabolite profiling of DK-GV-04P, a novel anticancer molecule under drug discovery Biomed. Chromatogr. (IF 1.8) Pub Date : 2023-12-12 Deeki Doma Sherpa, Amit Kumar Sahu, Tarang Jadav, Niraj Rajput, Gargi Nikhil Vaidya, Dinesh Kumar, Pinaki Sengupta
DK-GV-04P, chemically identified as 3-cinnamyl-2-(4-methoxyphenyl) quinazolin-4(3H)-one, is an investigational molecule synthesized at the Chemical Biology Laboratory of the National Institute of Pharmaceutical Education and Research-Ahmedabad. The compound has shown potential anticancer activity against squamous CAL27 cell lines. Metabolite identification and characterization are critical in drug