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The pathology according to p53 pathway. Pathobiology (IF 5.0) Pub Date : 2023-11-14 Yuichiro Hatano
Background Observations play a pivotal role in the progress of science, including in pathology. The cause of a disease such as cancer is analyzed by breaking it down into smaller organs, tissues, cells, and molecules. The current standard cancer diagnostic procedure, microscopic observation, relies on preserved morphological characteristics. In contrast, molecular analyses explore oncogenic pathway
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CD15 is a risk predictor and a novel target in clear cell renal cell carcinoma. Pathobiology (IF 5.0) Pub Date : 2023-11-14 Philipp Joachim Stenzel,Mario Schindeldecker,Larissa Seidmann,Esther Herpel,Markus Hohenfellner,Gencay Hatiboglu,Sebastian Foersch,Stefan Porubsky,Stephan Macher-Goeppinger,Wilfried Roth,Katrin Elisabeth Tagscherer
INTRODUCTION Tumor cells use adhesion molecules like CD15 or sialylCD15 (sCD15) for metastatic spreading. We analyzed the expression of CD15 and sCD15 in clear cell renal cell carcinoma (ccRCC) regarding prognosis. METHODS A tissue microarray containing tissue specimens of 763 patients with ccRCC was immunohistochemically stained for CD15 and sCD15, their expression quantified using digital image analysis
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Epithelial carcinomas arising within phyllodes tumours of the breast: a review of their histopathological, pathophysiological and molecular characteristics. Pathobiology (IF 5.0) Pub Date : 2023-11-11 William Cook,Cheok Soon Lee,Puay Hoon Tan
Epithelial proliferation is a common feature of phyllodes tumours (PT) but epithelial malignancy is rare. This review seeks to further our understanding of epithelial malignancy within phyllodes tumours by analysing their histopathological and molecular characteristics in previously reported cases in the literature. PubMed was searched for case reports, case series and literature reviews of in situ
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Exploration of the tumor biological significance of PCLO in gastric cancer - Results from a large Central European cohort. Pathobiology (IF 5.0) Pub Date : 2023-11-07 Maximilian Bernhardt,Hans-Michael Behrens,Sandra Krüger,Christoph Röcken
INTRODUCTION A recent multiregional whole exome sequencing of 48 tumour samples from 9 gastric adenocarcinomas discovered PCLO mutations in 23 (47.9%) tumour samples. Based on that unexpected high prevalence of PCLO mutations, we hypothesized a tumour biological significance of PCLO in gastric cancer (GC). METHODS Tumour samples (whole tissue sections) obtained from 466 patients resected for therapy-naive
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Exploring the expression of Adenosine Pathway-Related Markers CD73 and CD39 in Colorectal and Pancreatic Carcinomas Characterized by Multiplex Immunofluorescence: A Pilot Study. Pathobiology (IF 5.0) Pub Date : 2023-11-03 Cibelle Freitas Lima,Auriole Tamegnon,Saxon Rodriguez,Dipen Maru,Philip L Martin,Zachary A Cooper,Jaime Rodriguez-Canales,Edwin Roger Parra
INTRODUCTION Generating high levels of immunosuppressive adenosine in the tumor microenvironment contributes to cancer immune evasion. CD39 and CD73 hydrolyze adenosine triphosphate into adenosine; thus, efforts have been made to target this pathway for cancer immunotherapy. Our objective was optimizing a multiplex immunofluorescence (mIF) panel to explore the role of CD39 and CD73 within the tumor
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Gastric polyps in Familial Adenomatous Polyposis Portuguese patients: the first Western cohort with Asian features. Pathobiology (IF 5.0) Pub Date : 2023-10-18 Diana Baptista,Marco Fernandes,Mónica Garrido,Fabiana Sousa,Rui Morais,Jose Garcia-Pelaez,Roberto Silva,Dina Leitão,Manuela Baptista,José Barbosa,Fatima Carneiro,Irene Gullo
INTRODUCTION Chronic atrophic gastritis may contribute to gastric polyps (GP) phenotype in familial adenomatous polyposis (FAP). Considering the high prevalence of Helicobacter-pylori (HP) infection in Portugal, we aim to characterise GP in a series of Portuguese patients. METHODS In a retrospectively-selected series of 53 FAP patients, clinical data and histopathological features of GP and background
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Current State and Future Prospects of Diagnosis and Management of TP53 Mutated Myeloid Neoplasms. Pathobiology (IF 5.0) Pub Date : 2023-10-13 Sangeetha Venugopal,Sanam Loghavi
TP53 mutated myeloid neoplasms including acute myeloid leukemia (AML) and myelodysplastic neoplasms (MDS) are notoriously treatment resistant with uniformly poor outcomes. TP53 status is an important prognostic indicator and early knowledge of the of TP53 mutation/allelic state may assist in appropriate management including clinical trial enrollment for eligible patients. Thus far, no therapy has shown
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Discrepancies in Hormone Receptor and HER2 Expression between Malignant Serous Effusions and Paired Tissues from Primary or Recurrent Breast Cancers. Pathobiology (IF 5.0) Pub Date : 2023-10-10 Ilias P Nikas,Sojung Lim,Seock-Ah Im,Kyung-Hun Lee,Dae-Won Lee,Hyebin Lee,Han Suk Ryu
OBJECTIVES Immunohistochemistry (IHC) for the estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) biomarkers has prognostic and therapeutic value in breast cancer, while it facilitates molecular subtyping. This study aimed to identify subtype discordance and its clinical significance among different phases of breast cancer evolution, focusing on effusion
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Overexpression of VSNL1 enhances cell proliferation in colorectal carcinogenesis. Pathobiology (IF 5.0) Pub Date : 2023-10-05 Takayuki Aiba,Naoki Hijiya,Tomonori Akagi,Yoshiyuki Tsukamoto,Yuka Hirashita,Keisuke Kinoshita,Tomohisa Uchida,Chisato Nakada,Shusaku Kurogi,Yoshitake Ueda,Hidefumi Shiroshita,Norio Shiraishi,Kazunari Murakami,Masafumi Inomata,Masatsugu Moriyama
INTRODUCTION We have previously reported that overexpression of VSNL1 (Visinin-like protein 1) is frequently observed in advanced colorectal adenocarcinomas and correlates with poorer prognosis. In this study, we determined the levels of VSNL1 expression in the earlier stages of colorectal tumors including adenomas and adenocarcinomas, and attempted to clarify the functional significance of VSNL1 overexpression
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IMP3 immunohistochemical expression is related with progression and metastases in xenografted and cutaneous melanomas. Pathobiology (IF 5.0) Pub Date : 2023-10-05 Natividad Martin-Morales,Miguel Padial-Molina,Isabel Tovar,Virginea De Araujo Farias,Pedro Hernández-Cortés,Esperanza Ramirez-Moreno,Mercedes Caba-Molina,Justin Davis,Alejandro Carrero Castaño,Jose Mariano Ruiz de Almodovar,Pablo Galindo-Moreno,Javier Oliver-Pozo,Francisco Javier O'Valle Ravassa
INTRODUCTION Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP3) over-expression is a predictor of tumor recurrence and metastases in some types of human melanoma. Our objective is to evaluate the immunohistochemical expression of IMP3 and other molecules related to tumor prognosis in melanoma-xeno-tumors undergoing treatment. MATERIAL AND METHODS We inoculated A375 and G361
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Next generation sequencing of breast cancer in the neoadjuvant setting. Pathobiology (IF 5.0) Pub Date : 2023-09-01 Alexandra Sofia Amaral Mesquita,Anabela Ferro,José Carlos Machado,Fernando Schmitt
INTRODUCTION Many patients with locally advanced breast cancer are proposed to neoadjuvant chemotherapy (NAT) before surgery. Only some of them achieve a pathological complete response (pCR). The determination of gene somatic alterations using Next Generation Sequencing (NGS) in the non-pCR tumors is important, in order to identify potential opportunities of treatment for the patients, if targeted
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Origin of residual tumor masses in BRCA1/2-driven ovarian carcinomas treated by neoadjuvant chemotherapy: selection of preexisting BRCA1/2-proficient tumor cells but not the gain of second ORF-restoring mutation. Pathobiology (IF 5.0) Pub Date : 2023-08-14 Anna Sokolenko,Elena Preobrazhenskaya,Claudia Marchetti,Alessia Piermattei,Fedor Zagrebin,Ekatherina Kuligina,Tatiana Gorodnova,Matteo Pavone,Alexandr Ivantsov,Ilya Bizin,Giovanni Scambia,Igor Berlev,Anna Fagotti,Evgeny Imyanitov
INTRODUCTION Tubo-ovarian carcinomas (OCs) are highly sensitive to platinum-based neoadjuvant chemotherapy (NACT) but almost never demonstrate complete pathologic response. METHODS We analyzed paired primary and residual tumor tissues from 30 patients with hereditary BRCA1/2-driven OCs (BRCA1: 17; BRCA2: 13), who were treated by carboplatin/paclitaxel NACT (median number of cycles: 3, range 3-6). BRCA1/2
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Differential Gene Expression of SARS-CoV-2 positive Bronchoalveolar Lavages: A Case Series. Pathobiology (IF 5.0) Pub Date : 2023-07-25 Jasmin D Haslbauer,Spasenija Savic Prince,Anna K Stalder,Matthias S Matter,Carl P Zinner,Kathleen Jahn,Ellen Obermann,Jasmin Hanke,Karoline Leuzinger,Hans H Hirsch,Alexandar Tzankov
Transcriptomic data on bronchoalveolar lavage (BAL) from COVID-19 patients are currently scarce. This case series seeks to characterize the intraalveolar immunopathology of COVID-19. BALs were performed on 14 patients (5 COVID-19, of which 3 mild and 2 largely asymptomatic, 9 controls). Controls included asthma (n=1), unremarkable BALs (n=3), infections with respiratory syncytial virus (n=1), influenza
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Detection of Hybrid Fusion Transcripts, Aberrant Transcript Expression and Specific Single Nucleotide Variants in Acute Leukemia and Myeloid Disorders with Recurrent Gene Rearrangements. Pathobiology (IF 5.0) Pub Date : 2023-07-25 Yuewei Li,Kaifang Deng,Justin Kaner,Julia T Geyer,Madhu Ouseph,Frank Fang,Kemin Xu,Gail Roboz,Michael J Kluk
INTRODUCTION A variety of gene rearrangements and molecular alterations are key drivers in the pathobiology of acute leukemia and myeloid disorders; current classification systems increasingly incorporate these findings in diagnostic algorithms. Therefore, clinical laboratories require versatile tools, which can detect an increasing number and variety of molecular and cytogenetic alterations of clinical
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Genetic factors in familial manifestation of primary mediastinal large B-cell lymphoma over two generations. Pathobiology (IF 5.0) Pub Date : 2023-07-25 Darius Juskevicius,Pontus Lundberg,Alexandar Tzankov,Stefan Dirnhofer,Frank Stenner
INTRODUCTION Primary mediastinal large B-cell lymphoma (PMBL) is a rarely occurring lymphoid malignancy, which typically affects young adults and presents itself as an anterior mediastinal mass. Gene expression profiling, as well as somatic genetic analysis revealed that it is closely related to classical Hodgkin lymphoma, whereas morphologically it tends to resemble diffuse large B-cell lymphoma.
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Critical Evaluation of Transcripts and Long Noncoding RNA Expression Levels in Prostate Cancer Following Radical Prostatectomy. Pathobiology (IF 5.0) Pub Date : 2023-07-18 Christian Ruiz,Ilaria Alborelli,Massimilinao Manzo,Byron Calgua,Eveline Barbara Keller,Vincent Vuaroqueaux,Luca Quagliata,Cyrill A Rentsch,Giulio Cesare Spagnoli,Pierre André Diener,Lukas Bubendorf,Rudolf Morant,,Serenella Eppenberger-Castori
INTRODUCTION The clinical course of prostate cancer (PCa) is highly variable, ranging from indolent behavior to rapid metastatic progression. The Gleason score is widely accepted as the primary histologic assessment tool with significant prognostic value. However, additional biomarkers are required to better stratify patients, particularly those at intermediate risk. METHODS In this study, we analyzed
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Expression profile of microenvironmental factors in the interface zone of colorectal cancer: histological-stromal biomarkers and cancer cell-CAF-related proteins combined for the assessment of tumor progression. Pathobiology (IF 5.0) Pub Date : 2023-06-27 Ricella Maria Souza da Silva,Eduardo Moreira Queiroga,Cynthia Aparecida Bueno de Toledo Osório,Karin S Cunha,Fabiana P Neves,Julieth P Andrade,Eliane P Dias
Introduction The characterization of tumor microenvironment (TME) related-factors and their impact on tumor progression have attracted much interest. We investigated cancer cells and CAFs to evaluate biomarkers that are associated with neoplastic progression, observing them in different interface zones of colorectal cancer. Methods On 357 CRC tissue microarrays, using immunohistochemistry, we examined
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Hepatic fat and macrophages are increased in livers of diabetic patients without NAFLD. Pathobiology (IF 5.0) Pub Date : 2023-06-14 Amber Korn,Cacharel Nadeem,Emma N Bos,Hans Wm Niessen,Suat Simsek,Paul Aj Krijnen
AIMS Diabetes mellitus (DM), especially type 2, is strongly associated with non-alcoholic fatty liver disease (NAFLD). Recent studies indicate that particularly in DM patients, "simple" liver steatosis can progress into more severe disease. However, little is known about putative hepatic histopathological changes in DM patients without NAFLD. In this study we therefore analysed fat content and inflammatory
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Pediatric Hematopathology in the era of advanced molecular diagnostics: what we know and how we can apply the updated classifications. Pathobiology (IF 5.0) Pub Date : 2023-06-13 Yen-Chun Liu,Julia T Geyer
Pediatric hematologic malignancies often show genetic features distinct from their adult counterparts, which reflects the differences in their pathogenesis. Advances in the molecular diagnostics including the wide use of next-generation sequencing (NGS) technology have revolutionized the diagnostic workup for hematologic disorders and led to the identification of new disease subgroups as well as prognostic
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Hepatic progenitor cells in the form of ductular structures within a GIST liver metastasis: supporting a putative role in the hepatic metastatic niche. Pathobiology (IF 5.0) Pub Date : 2023-06-08 João Lobo,João Gama,Rita Luis,Dina Tiniakos,Maria Augusta Cipriano
Recent studies have highlighted the presence of hepatic progenitor cells (HPCs) in metastatic liver carcinomas. We provide further evidence of this phenomenon, presenting a case of a gastrointestinal stromal tumour (GIST) liver metastasis with evidence of intra- and peritumoral HPC. A 64-year-old man presented with a gastric mass diagnosed as a high-risk KIT-mutated GIST. The patient was treated with
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Performance of immunohistochemical and molecular methods in detecting microsatellite instability in gastric cancer: a multicenter study. Pathobiology (IF 5.0) Pub Date : 2023-06-02 Diogo Sousa Marques,Irene Gullo,Luís Mascarenhas-Lemos,João Ricardo Silva,Catarina Neto do Nascimento,Patrícia Pontes,Lídia Pinho,Luis Cirnes,Xiaogang Wen,Marília Cravo,Fátima Carneiro
INTRODUCTION Introduction: Microsatellite instability (MSI) is an important prognostic molecular biomarker for gastric cancer (GC). MSI status may be detected by immunohistochemistry (IHC) for mismatch repair (MMR) proteins and polymerase chain reaction (PCR). Idylla MSI assay has not been validated for GC but may prove to be a valid alternative. METHODS In a series of 140 GC cases, MSI status was
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Progression in Myeloid Neoplasms: Beyond the Myeloblast. Pathobiology (IF 5.0) Pub Date : 2023-05-11 Carlos Faria,Alexandar Tzankov
Disease progression in myelodysplastic syndromes (MDS), myelodysplastic-myeloproliferative neoplasms (MDS/MPN), and myeloproliferative neoplasms (MPN), altogether referred to as myeloid neoplasms (MN), is a major source of mortality. Apart from transformation to acute myeloid leukemia, the clinical progression of MN is mostly due to the overgrowth of pre-existing hematopoiesis by the MN without an
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Distribution and clinical significance of HPV16 variants in head and neck squamous cell carcinomas: data from a Portuguese cohort and systematic review Pathobiology (IF 5.0) Pub Date : 2023-04-11 DANIELA COCHICHO, Alexandra Nunes, Daniel Sobral, João P. Gomes, Susana Esteves, Joana Mendonça, Luis Vieira, Luís Martins, Mario Cunha, Pedro Montalvão, Miguel Magalhães, Rui M. Gil da Costa, Ana Maria Félix
Introduction: Genomic variants of the human papillomavirus type 16 (HPV16) are thought to play differential roles in the susceptibility to head and neck squamous cell carcinomas (HNSCC) and its biological behaviour. This study aims to establish the prevalence of HPV16 variants in an HNSCC cohort and associate them with clinical-pathological characteristics and patient survival. Methods: We retrieved
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High Inner Centromere Protein (INCENP) expression correlates with aggressive features and predicts poor prognosis in patients with invasive breast cancer Pathobiology (IF 5.0) Pub Date : 2023-04-07 Asmaa Ibrahim, Islam M. Miligy, Michael S. Toss, Andrew R. Green, Emad A. Rakha
Background: Inner centromere protein (INCENP) is a member of the chromosomal passenger complex (CPC) and plays a key role in mitosis and cell proliferation. This study aims to evaluate the clinical and prognostic significance of INCENP in invasive breast cancer (BC). Material and Methods: INCENP protein expression was evaluated on a tissue microarray of a large BC cohort (n=1295) using immunohistochemistry
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Disruptive and truncating TP53 mutations are associated with African-ancestry and worse prognosis in Brazilian patients with lung adenocarcinoma Pathobiology (IF 5.0) Pub Date : 2023-04-08 Rodrigo de Oliveira Cavagna, Icaro Alves Pinto, Flávia Escremim de Paula, Gustavo Noriz Berardinelli, Débora Sant'Anna, Iara Santana, Vinicius Duval Da Silva, Eduardo Caetano Albino da Silva, José Elias Miziara, Josiane Mourao, Augusto Antoniazzi, Alexandre Jacinto, Pedro De Marchi, Miguel Angel Molina, Leticia Ferro Leal, Rui Manuel Reis
INTRODUCTION: TP53 is the most frequently mutated gene in lung tumors, but its prognostic role in admixed populations, such as Brazilians, remains unclear. In this study, we aimed to evaluate the frequency and clinicopathological impact of TP53 mutations in non-small cell lung cancer (NSCLC) patients in Brazil. METHODS: We analyzed 446 NSCLC patients from Barretos Cancer Hospital. TP53 mutational status
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Senescent tumor cells are frequently present at the invasion front - implications for improving disease control in patients with locally advanced prostate cancer Pathobiology (IF 5.0) Pub Date : 2023-03-31 Sebastian Schwarz, Cathleen Nientiedt, Elena-Sophie Prigge, Adam Kaczorowski, Christine Geisler, Carlota Lucena Porcel, Magnus von Knebel Doeberitz, Markus Hohenfellner, Stefan Duensing
Introduction: Local tumor invasion is a critical factor for the outcome of men with prostate cancer. In particular seminal vesicle invasion (SVI) has been reported to be associated with a more unfavorable prognosis. A better understanding of the functional state of invading prostate cancer cells is crucial to develop novel therapeutic strategies for patients with locally advanced disease. Methods: The
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Primary Effusion Lymphoma in an HIV-Negative Patient with Chronic Myeloid Leukemia Treated with Dasatinib Pathobiology (IF 5.0) Pub Date : 2023-03-30 Ivo N. SahBandar, Chandler B. Sy, Tayler van den Akker, David Kim, Julia T. Geyer, Amy Chadburn, Ethel Cesarman, Giorgio Inghirami, John Allan, Momin T. Siddiqui, Madhu M. Ouseph
Introduction: Primary effusion lymphoma (PEL) is a malignant lymphomatous effusion, which by definition is Kaposi sarcoma herpesvirus/human herpesvirus 8-positive. PEL typically occurs in HIV-infected patients, but can also occur in HIV-negative individuals, including in organ transplant recipients. Tyrosine kinase inhibitors (TKIs) are currently the standard of care for patients with chronic myeloid
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Myeloid proliferations associated with Down syndrome: clinicopathologic characteristics of forty cases from five large academic institutions Pathobiology (IF 5.0) Pub Date : 2023-03-30 Tayler A van den Akker, Yen-Chun Liu, Huifei Liu, Jennifer Chapman, Jennifer Levine, Olga Weinberg, Julia T. Geyer
Objectives: The incidence of myelodysplastic syndrome and acute myeloid leukemia is significantly increased in children with Down syndrome (DS). Within the revised 2016 WHO edition, these entities are jointly classified as myeloid leukemia associated with DS (ML-DS). Additionally, infants with DS may develop transient abnormal myelopoiesis (TAM) which is histomorphologically identical to ML-DS. While
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NPM1-mutated acute myeloid leukemia: recent developments and open questions Pathobiology (IF 5.0) Pub Date : 2023-03-21 Sanjay S. Patel
Somatic mutations in the nucleophosmin (NPM1) gene occur in approximately 30% of de novo acute myeloid leukemias (AML), and are relatively enriched in normal karyotype (NK) AMLs. Earlier World Health Organization (WHO) classification schema recognized NPM1-mutated AMLs as a unique subtype of AML, while the latest WHO and International Consensus Classification (ICC) now consider NPM1 mutations as AML-defining
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Artificial intelligence in bone marrow histological diagnostics: potential applications and challenges Pathobiology (IF 5.0) Pub Date : 2023-02-15 Leander van Eekelen, Geert Litjens, Konnie Hebeda
The expanding digitalization of routine diagnostic histological slides holds a potential to apply artificial intelligence (AI) to pathology, including bone marrow (BM) histology. In this perspective we describe potential tasks in diagnostics that can be supported, investigations that can be guided and questions that can be answered by the future application of AI on whole slide images of BM biopsies
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Artificial Intelligence in Bone Marrow Histological Diagnostics: Potential Applications and Challenges Pathobiology (IF 5.0) Pub Date : 2023-02-15
The expanding digitalization of routine diagnostic histological slides holds a potential to apply artificial intelligence (AI) to pathology, including bone marrow (BM) histology. In this perspective, we describe potential tasks in diagnostics that can be supported, investigations that can be guided, and questions that can be answered by the future application of AI on whole-slide images of BM biopsies
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Guidelines for Handling of Cytological Specimens in Cancer Genomic Medicine Pathobiology (IF 5.0) Pub Date : 2023-02-08 Eiichi Morii, Yutaka Hatanaka, Noriko Motoi, Akihiko Kawahara, Shinji Hamakawa, Takeshi Kuwata, Tadasuke Nagatomo, Yoshinao Oda, Aikou Okamoto, Ryota Tanaka, Akira Iyoda, Maeda Ichiro, Yukiko Matsuo, Nobuyuki Nakamura, Tokiko Nakai, Mei Fukuhara, Kazuya Tokita, Tomohiko Yamaguchi, Masataka Takenaka, Ayako Kawabata, Kanako C. Hatanaka, Kaho Tsubame, Yukitoshi Satoh, The Working Group on Cytology in
Rapid advances are being made in cancer drug therapy. Since molecularly targeted therapy has been introduced, personalized medicine is being practiced, pathological tissue from malignant tumors obtained during routine practice is frequently used for genomic testing. Whereas cytological specimens fixed mainly in alcohol are considered to be more advantageous in terms of preservation of the nucleic acid
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Guidelines for Handling of Cytological Specimens in Cancer Genomic Medicine Pathobiology (IF 5.0) Pub Date : 2023-02-08
Rapid advances are being made in cancer drug therapy. Since molecularly targeted therapy has been introduced, personalized medicine is being practiced, pathological tissue from malignant tumors obtained during routine practice is frequently used for genomic testing. Whereas cytological specimens fixed mainly in alcohol are considered to be more advantageous in terms of preservation of the nucleic acid
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Blood Liquid Biopsy in an Advanced Medullary Thyroid Carcinoma: A Case Study with Rearranged during Transfection Heterogeneity Pathobiology (IF 5.0) Pub Date : 2023-02-02 Josep Gumà, Karla Beatríz Peña, Francesc Riu, Clara Lucia-Gozálvez, Ana Vidaller, Vanesa Maldonado, David Parada
Introduction: Liquid biopsy is an innovative and efficient method for studying circulating tumor DNA. In conjunction with innovative techniques such as next-generation sequencing, it can provide real-time information on prognostic and predictive factors. Case Presentation: We report a case of advanced, unresectable medullary thyroid carcinoma with various rearranged during transfection (RET) and Kirsten
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Blood Liquid Biopsy in an Advanced Medullary Thyroid Carcinoma: A Case Study with Rearranged during Transfection Heterogeneity Pathobiology (IF 5.0) Pub Date : 2023-02-02
Introduction: Liquid biopsy is an innovative and efficient method for studying circulating tumor DNA. In conjunction with innovative techniques such as next-generation sequencing, it can provide real-time information on prognostic and predictive factors. Case Presentation: We report a case of advanced, unresectable medullary thyroid carcinoma with various rearranged during transfection (RET) and Kirsten
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miR-142-3p Suppresses Invasion and Adhesion of Mesothelioma Cells by Downregulating ITGAV Pathobiology (IF 5.0) Pub Date : 2023-02-01 Ihiro Endo, Vishwa Jeet Amatya, Kei Kushitani, Tetsuya Nakagiri, Kohei Aoe, Yukio Takeshima
Introduction: Malignant mesothelioma is an aggressive cancer associated with asbestos exposure. Currently, the efficacy of therapeutics is limited in malignant mesothelioma, and developing more effective therapies is the need of the hour. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), have attracted attention as therapeutic targets. To explore potential therapeutic targets, we focused on miR-142-3p
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Clinical Value of Platelets and Coagulation Parameters in Predicting the Severity of Delta Variant SARS-CoV-2 Pathobiology (IF 5.0) Pub Date : 2023-02-01 Yue-e Chen, Fu-le Ren, Xing Gu, Hong-jun Zhang, Wen-jie Li, Han Yang, Fen-qing Shang
Introduction: The present study aimed to analyze the clinical features and laboratory markers of patients with Delta variant SARS-CoV-2 and explore the role of platelet in predicting the severity of Delta. Methods: This retrospective, observational study was conducted on 863 patients laboratory-confirmed Delta variant SARS-CoV-2. These cases were sub-classified based on disease severity into mild (n
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Clinical and Prognostic Impact of the Warburg Effect in Esophageal Carcinoma: Monocarboxylate Transporters as Candidates for Therapeutic Targeting Pathobiology (IF 5.0) Pub Date : 2023-02-01
Introduction: Esophageal cancer (EC) seems to display increased glycolytic activity, but clinical studies on the expression/prognostic significance of glycometabolism-related proteins, as well as functional assays, are missing. Methods: Expression of 10 glycolytic biomarkers was evaluated by immunohistochemistry in tissue sections from 95 patients. Two esophageal squamous cell carcinoma (ESCC) cell
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miR-142-3p Suppresses Invasion and Adhesion of Mesothelioma Cells by Downregulating ITGAV Pathobiology (IF 5.0) Pub Date : 2023-02-01
Introduction: Malignant mesothelioma is an aggressive cancer associated with asbestos exposure. Currently, the efficacy of therapeutics is limited in malignant mesothelioma, and developing more effective therapies is the need of the hour. Non-coding RNAs (ncRNAs), including microRNAs (miRNAs), have attracted attention as therapeutic targets. To explore potential therapeutic targets, we focused on miR-142-3p
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Clinical Value of Platelets and Coagulation Parameters in Predicting the Severity of Delta Variant SARS-CoV-2 Pathobiology (IF 5.0) Pub Date : 2023-02-01
Introduction: The present study aimed to analyze the clinical features and laboratory markers of patients with Delta variant SARS-CoV-2 and explore the role of platelet in predicting the severity of Delta. Methods: This retrospective, observational study was conducted on 863 patients laboratory-confirmed Delta variant SARS-CoV-2. These cases were sub-classified based on disease severity into mild (n
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Clinical and Prognostic Impact of the Warburg Effect in Esophageal Carcinoma: Monocarboxylate Transporters as Candidates for Therapeutic Targeting Pathobiology (IF 5.0) Pub Date : 2023-02-01
Introduction: Esophageal cancer (EC) seems to display increased glycolytic activity, but clinical studies on the expression/prognostic significance of glycometabolism-related proteins, as well as functional assays, are missing. Methods: Expression of 10 glycolytic biomarkers was evaluated by immunohistochemistry in tissue sections from 95 patients. Two esophageal squamous cell carcinoma (ESCC) cell
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Cancer registration, molecular marker status and adherence to the WHO 2016 classification of pathology reports for glioma diagnosed during 2017-2019 in Belgium Pathobiology (IF 5.0) Pub Date : 2023-01-26
Introduction: The objective of this study is to cross-check and if necessary, adjust registered ICD-O-3 topography and morphology codes with the findings in pathology reports available at the Belgian Cancer Registry (BCR) for glioma patients. Additionally, integration of molecular markers in the pathological diagnosis and concordance with WHO 2016 classification is investigated. Methods: Since information
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Cancer registration, molecular marker status and adherence to the WHO 2016 classification of pathology reports for glioma diagnosed during 2017-2019 in Belgium Pathobiology (IF 5.0) Pub Date : 2023-01-26
Introduction: The objective of this study is to cross-check and if necessary, adjust registered ICD-O-3 topography and morphology codes with the findings in pathology reports available at the Belgian Cancer Registry (BCR) for glioma patients. Additionally, integration of molecular markers in the pathological diagnosis and concordance with WHO 2016 classification is investigated. Methods: Since information
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Renal cell carcinoma in end-stage renal disease: A retrospective study in patients from Hungary Pathobiology (IF 5.0) Pub Date : 2023-01-25 Dávid Semjén, Borbála Dénes, Áron Somorácz, Attila Fintha, Gertrúd Forika, Alex Jenei, Deján Dobi, Tamás Micsik, Kornélia Veronika Eizler, Nándor Giba, Fanni Sánta, Anita Sejben, Béla Iványi, Levente Kuthi
Introduction: End-stage renal disease (ESRD) and acquired cystic kidney disease (ACKD) are known risk factors for renal cell carcinoma (RCC). Hereby, the clinicopathological features of RCCs developed in ESRD were investigated. Methods: A database consisting of 34 tumors from 31 patients with ESRD among 2 566 nephrectomy samples of RCC was built. The demographic, clinical, and follow-up data along
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Renal cell carcinoma in end-stage renal disease: A retrospective study in patients from Hungary Pathobiology (IF 5.0) Pub Date : 2023-01-25
Introduction: End-stage renal disease (ESRD) and acquired cystic kidney disease (ACKD) are known risk factors for renal cell carcinoma (RCC). Hereby, the clinicopathological features of RCCs developed in ESRD were investigated. Methods: A database consisting of 34 tumors from 31 patients with ESRD among 2 566 nephrectomy samples of RCC was built. The demographic, clinical, and follow-up data along
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GATA3 Expression in Human Tumors: A Tissue Microarray Study on 16,557 Tumors Pathobiology (IF 5.0) Pub Date : 2023-01-17 Viktor Reiswich, Carol E. Schmidt, Maximilian Lennartz, Doris Höflmayer, Claudia Hube-Magg, Sören Weidemann, Christoph Fraune, Franziska Büscheck, Katharina Möller, Christian Bernreuther, Ronald Simon, Till S. Clauditz, Niclas C. Blessin, Elena Bady, Guido Sauter, Ria Uhlig, Stefan Steurer, Sarah Minner, Eike Burandt, David Dum, Andreas H. Marx, Till Krech, Patrick Lebok, Andrea Hinsch, Frank Jacobsen
Introduction: GATA3 is a transcription factor involved in epithelial cell differentiation. GATA3 immunostaining is used as a diagnostic marker for breast and urothelial cancer but can also occur in other neoplasms. Methods: To evaluate GATA3 in normal and tumor tissues, a tissue microarray containing 16,557 samples from 131 different tumor types and subtypes and 608 samples of 76 different normal tissue
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GATA3 Expression in Human Tumors: A Tissue Microarray Study on 16,557 Tumors Pathobiology (IF 5.0) Pub Date : 2023-01-17
Introduction: GATA3 is a transcription factor involved in epithelial cell differentiation. GATA3 immunostaining is used as a diagnostic marker for breast and urothelial cancer but can also occur in other neoplasms. Methods: To evaluate GATA3 in normal and tumor tissues, a tissue microarray containing 16,557 samples from 131 different tumor types and subtypes and 608 samples of 76 different normal tissue
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Evaluation of the Implementation and Diagnostic Accuracy of the Paris Classification for Reporting Urinary Cytology in Voided Urine Specimens: A Cyto-Histological Correlation Study in a Cancer Center Pathobiology (IF 5.0) Pub Date : 2022-12-27 João Lobo, Cláudia Lobo, Luís Leça, Ângelo Rodrigues, Rui Henrique, Paula Monteiro
Introduction: The Paris classification highlights the need to focus on accurately identifying high-grade urothelial carcinoma (HGUC). Herein, we aimed to assess the overall implementation and diagnostic performance of the Paris classification for reporting urinary cytology in a cancer center. Methods: All urinary cytology reports from July 2018 to December 2019 were collected (n = 1,240). Only voided
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Evaluation of the Implementation and Diagnostic Accuracy of the Paris Classification for Reporting Urinary Cytology in Voided Urine Specimens: A Cyto-Histological Correlation Study in a Cancer Center Pathobiology (IF 5.0) Pub Date : 2022-12-27
Introduction: The Paris classification highlights the need to focus on accurately identifying high-grade urothelial carcinoma (HGUC). Herein, we aimed to assess the overall implementation and diagnostic performance of the Paris classification for reporting urinary cytology in a cancer center. Methods: All urinary cytology reports from July 2018 to December 2019 were collected (n = 1,240). Only voided
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Flow Cytometry Analysis Reveals a Wide Cytologic and Immunophenotypic Spectrum of Peripheral B Lymphocytes in Angioimmunoblastic T-Cell Lymphoma Pathobiology (IF 5.0) Pub Date : 2022-11-24 Hung-Lin Liu, Chun-Kai Liao, Shao-Wen Weng, Lee-Yung Shih, Chih-Chi Chou, Huey-Ling You, Ming-Chung Wang, Wan-Ting Huang
Introduction: Angioimmunoblastic T-cell lymphoma (AITL) is an aggressive T-cell lymphoma commonly associated with B-cell dysregulation. Correlations involving B-cell dysregulation and clinicopathological features remain unclear. Methods: We prospectively collected blood samples from 11 AITL patients and 17 healthy controls. The percentages of B-cell subpopulations and lymphocytes with IL-21 production
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Lymphocyte Subsets in Cervicovaginal Lavage Specimens of HIV-Infected Women: A Surrogate Risk Marker of HPV-Associated Cervical Lesions Pathobiology (IF 5.0) Pub Date : 2022-10-27 Maria Jose Brito, Ana Quintas, Filipa Passos, Ana Teresa Alves, Ana Félix, Catarina Gregório Martins
Introduction: To better understand the role of mucosa immunity in the development of cervical carcinoma in HIV infection, cervical lymphocyte subsets were characterized in HIV+ and HIV− women, as well as their relation to HPV-associated cervical lesions. Methods: Eighty-three (52 HIV+, 31 HIV−) cell suspensions of cervicovaginal lavage (CVL) and 52 HIV+ peripheral blood (PB) samples were assessed by
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Prevalence of Molecular Alterations in a Swiss Cohort of 512 Colorectal Carcinoma Patients by Targeted Next-Generation Sequencing Analysis in Routine Diagnostics Pathobiology (IF 5.0) Pub Date : 2022-10-06 Simon Haefliger, Katharina Marston, Ilaria Alborelli, Edouard-Jean Stauffer, Mathias Gugger, Philip M. Jermann, Sylvia Hoeller, Luigi Tornillo, Luigi M. Terracciano, Michel Bihl, Matthias S. Matter
Introduction: Colorectal carcinoma (CRC) is among the most common carcinomas in women and men. In the advanced stage, patients are treated based on the RAS status. Recent studies indicate that in the future, in addition to KRAS and NRAS, alterations in other genes, such as PIK3CA or TP53, will be considered for therapy. Therefore, it is important to know the mutational landscape of routinely diagnosed
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Evolutionary Changes in Pathology and Our Understanding of Disease Pathobiology (IF 5.0) Pub Date : 2022-08-23 Edwin Jun Chen Chew, Puay Hoon Tan
The history of pathology involves human dissections, autopsies, microscopy, as well as the development of histopathological, immunohistochemical, and molecular techniques. Through these methods, our understanding of anatomy and disease improved, and models of pathogenesis were gradually refined over time. This review discusses key milestones in the development of pathology as a branch of medical science
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A Comparison of Tissue Dissection Techniques for Diagnostic, Prognostic, and Theragnostic Analysis of Human Disease Pathobiology (IF 5.0) Pub Date : 2022-08-11 Elise M. Walsh, Marc K. Halushka
Histopathology has historically been the critical technique for the diagnosis and treatment of human disease. Today, genomics, transcriptomics, and proteomics from specific cells, rather than bulk tissue, have become key to understanding underlying disease mechanisms and rendering useful diagnostic information. Extraction of desired analytes, i.e., nucleic acids or proteins, from easily accessible
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The More Extensive the Spread through Air Spaces, the Worse the Prognosis Is: Semi-Quantitative Evaluation of Spread through Air Spaces in Pulmonary Adenocarcinomas Pathobiology (IF 5.0) Pub Date : 2022-08-10 Noémi Zombori-Tóth, Dóra Paróczai, Judit Lantos, Szintia Almási, Anita Sejben, László Tiszlavicz, Gábor Cserni, József Furák, Tamás Zombori
Introduction: The extent of spread through air spaces (STAS) is less investigated among patients with lung adenocarcinoma who underwent sublobar resection. Therefore, we aimed to evaluate the extent of STAS semi-quantitatively, to assess its prognostic impact on overall survival (OS) and recurrence-free survival (RFS), and to investigate the reproducibility of this assessment. Methods: The number of
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Erratum Pathobiology (IF 5.0) Pub Date : 2022-07-28 Fernando C. Schmitt, Bettina Borisch, Wataru Yasui, K. Zatloukal, Hiroshi Yokozaki, Abeer Shaaban, Sarah Pinder, Nicolò Costantino Brembilla, Stefan M. Willems, Anna Porwit, Alexander Katalinic, Wolfgang E. Fleig, Gerald Höfler, J.A. Kummer, Peh Suat-Cheng, Martin Werner, J. Van der Walt, S. Kumar, H.-Anton Lehr, C.J. Kirkpatrick, Pedro L. Fernandez, Han-Kwang Yang, Alexandar Tzankov, Jose A. Lorente
Pathobiology
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Autoimmune Thyroiditis Induced by Bartonella henselae (Cat-Scratch Disease) Might Be Reversible Pathobiology (IF 5.0) Pub Date : 2022-07-22 Marek Niedziela, Jarosław Szydlowski, Michal Dopierala, Jadwiga Maldyk, Iwona Klimecka, Pawel Kurzawa
Introduction: Bartonella henselae infection leads to development of cat-scratch disease (CSD) but may also trigger of autoimmune thyroiditis (AIT). Case Presentation: We describe a 4-year-old boy with a severe fever of unknown etiology, disseminated neck lymphadenopathy, and a headache. Treatment with antibiotics was employed, but finally a left tonsillectomy, selective left lymphadenectomy, and immunophenotyping