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Inhibition of PEDV viral entry upon blocking N-glycan elaboration Virology (IF 3.7) Pub Date : 2024-03-12 Yong Zhao, Tang Tao, Wenchang Zhao, Weiguang Fu, Tao Li
Porcine Epidemic Diarrhea Virus (PEDV) poses a significant threat to the global swine industry, demanding a thorough understanding of its cellular invasion mechanism for effective interventions. This study meticulously investigates the impact of O- and -linked glycans on PEDV proteins and host cell interaction, shedding light on their influence on the virus's invasion process.
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Human parainfluenza virus type 2 V protein inhibits mitochondrial apoptosis pathway through two ways Virology (IF 3.7) Pub Date : 2024-03-11 Naoki Saka, Machiko Nishio, Keisuke Ohta
Paramyxoviruses are reported to block apoptosis for their replication, but the mechanisms remain unclear. Furthermore, regulation of mitochondrial apoptosis by paramyxoviruses has been hardly reported. We investigated whether and how human parainfluenza virus type 2 (hPIV-2) counteracts apoptosis. Infection of recombinant hPIV-2 carrying mutated V protein showed higher caspase 3/7 activity and higher
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Natural hyperoside extracted from hawthorn exhibits antiviral activity against porcine epidemic diarrhea virus in vitro and in vivo Virology (IF 3.7) Pub Date : 2024-03-11 Jun Wang, Haibo Sun, Mingjun Su, Zijian Li, Lu Li, Feiyu Zhao, Yongchen Zhang, Wenfei Bai, Shiping Yu, Xu Yang, Shanshan Qi, Dan Yang, Donghua Guo, Chunqiu Li, Qinghe Zhu, Xiaoxu Xing, Dongbo Sun
Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and death in piglets, resulting in significant economic losses for the pork industry. There is an urgent need for new treatment strategies. Here, we focused on optimizing the process of purifying natural hyperoside (nHYP) from hawthorn and evaluating its effectiveness against PEDV both and . Our findings demonstrated that nHYP with a purity
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The investigation of the dynamics of changes in neutralizing antibody titers against type 5 adenovirus in the context of vaccination against a new coronavirus infection Virology (IF 3.7) Pub Date : 2024-03-08 I.V. Amosova, T.A. Timoshicheva, R.A. Kadyrova, Y.A. Zabrodskaya, V.S. Vakin, M.P. Grudinin, V.V. Dzytseva, M.S. Khmelevsky, D.A. Lioznov
This research focuses on analyzing the dynamics of neutralizing antibody (nAbs) titers against type 5 adenovirus (Ad5) in the adult population of Russia following vaccination against the novel coronavirus infection with recombinant adenovirus type-5 COVID-19 vaccine (CanSino Biologics, China). The impact of the Ad5 vector on nAb titers was investigated using 302 blood serum samples from individuals
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New perspective of small-molecule antiviral drugs development for RNA viruses Virology (IF 3.7) Pub Date : 2024-03-07 Shasha Li, Huixia Li, Ruiya Lian, Jingying Xie, Ruofei Feng
High variability and adaptability of RNA viruses allows them to spread between humans and animals, causing large-scale infectious diseases which seriously threat human and animal health and social development. At present, AIDS, viral hepatitis and other viral diseases with high incidence and low cure rate are still spreading around the world. The outbreaks of Ebola, Zika, dengue and in particular of
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Alum/CpG adjuvant promotes immunogenicity of inactivated SARS-CoV-2 Omicron vaccine through enhanced humoral and cellular immunity Virology (IF 3.7) Pub Date : 2024-03-07 Xiaotong Zheng, Rong Yang, Yuxiu Zhao, Yadan Zhang, Guangying Yuan, Weidong Li, Zhuangzhuang Xiao, Xiaofei Dong, Meng Ma, Yancen Guo, Wei Wang, Xue Zhao, Hongqiang Yang, Shaoting Qiu, Zheng Peng, Ankang Liu, Shouzhi Yu, Yuntao Zhang
The SARS-CoV-2 Omicron variant, which was classified as a variant of concern (VOC) by the World Health Organization on 26 November 2021, has attracted worldwide attention for its high transmissibility and immune evasion ability. The existing COVID-19 vaccine has been shown to be less effective in preventing Omicron variant infection and symptomatic infection, which brings new challenges to vaccine
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Saccharomyces cerevisiae cells that display norovirus P induce both systemic and mucosal neutralizing antibodies Virology (IF 3.7) Pub Date : 2024-03-06 Xin He, Nianzhu Jiang, Yaoming Li
The human norovirus (HuNov) is the leading cause of acute gastroenteritis (AGE) worldwide. Mucosal secretory IgA (sIgA) in the gastrointestinal tract interrupts the interaction between host cells and HuNov, thus inhibiting viral infection. In this study, we constructed a recombinant () expressing the HuNov P protein (GII. 4) and evaluated its immunogenicity in mice after oral delivery. First, the recombinant
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Transcriptome analysis of the synergistic mechanisms between two strains of potato virus Y in Solanum tuberosum L. Virology (IF 3.7) Pub Date : 2024-03-06 Liping Xu, Wei Zhang, Shangwu Liu, Yanling Gao, Yuanju Huang, Xianzhou Nie, Yanju Bai
Many viruses employ a process known as superinfection exclusion (SIE) to block subsequent entry or replication of the same or closely related viruses in the cells they occupy. SIE is also referred to as Cross-protection refers to the situation where a host plant infected by a mild strain of a virus or viroid gains immunity against a more severe strain closely related to the initial infectant. The mechanisms
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Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing Virology (IF 3.7) Pub Date : 2024-03-05 Yan Xie, Xianan Liu, Chaohu Luo, Qianqian Hu, Xuan Che, Liling Zhao, Min Zhao, Liqi Wu, Ming Ding
A begomovirus isolated from whiteflies () and tomato, sweet potato in China was found to be representative of a distinct begomovirus species, for which the name tomato yellow leaf curl Chuxiong virus (TYLCCxV) is proposed. The results of genomic identification and sequence comparison showed that TYLCCxV shares the highest complete nucleotide sequence identity (88.3%) with croton yellow vein mosaic
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The conserved RNP motif of the herpes simplex virus 1 family B DNA polymerase is crucial for viral DNA synthesis but not polymerase activity Virology (IF 3.7) Pub Date : 2024-03-03 Jessica L. Lawler, Shariya Terrell, Donald M. Coen
The herpes simplex virus 1 DNA polymerase contains a highly conserved structural motif found in most family B polymerases and certain RNA-binding proteins. To investigate its importance within cells, we constructed a mutant virus with substitutions in two residues of the motif and a rescued derivative. The substitutions resulted in severe impairment of plaque formation, yields of infectious virus,
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A comparative analysis of phage classification methods in light of the recent ICTV taxonomic revisions Virology (IF 3.7) Pub Date : 2024-02-29 Xihui Zhu, Luqi Tang, Zhiwei Wang, Feng Xie, Wei Zhang, Yubao Li
Recent ICTV taxonomy updates significantly changed phage taxonomy, yet a thorough phage classification workflow doesn't exist. This study compares six categorization tools and establishes a novel multi-method approach, combining genome similarity and specialized protein analysis. Applying the method to APEC phage P151 showed consistent categorization across platforms. A possible workflow for phage
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Characterization of Sf9 cell clones with differential susceptibilities to Sf-rhabdovirus X+3.7 and Sf-rhabdovirus X− replication Virology (IF 3.7) Pub Date : 2024-02-27 Hailun Ma, Andrea Kennard, Nicholas Mattson, Arifa S. Khan
Our laboratory previously discovered a novel rhabdovirus in the Sf9 insect cell line that was designated as Sf-rhabdovirus. Using limiting dilution, this cell line was found to be a mixed population of cells infected by Sf-rhabdovirus variants containing either the full length X accessory gene with a 3.7 kb internal duplication (designated as Sf-rhabdovirus X) or lacking the duplication and part of
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Preliminary report on therapeutic potential of coal-derived carbon quantum dots against SARS-CoV-2 virus Virology (IF 3.7) Pub Date : 2024-02-27 Binoy K. Saikia, Kallol Roy, Rituraj Konwar
Due to the pandemic of COVID-19 and subsequent emerging of new mutant strains, there has been a worldwide hunt for therapeutic and protective agents for its inhibition. In this short communication, for the first time, we report the coal-derived carbon quantum dot (CQD) for the possible therapeutic application against SARS-CoV-2. The synthesized C1-CQD is observed to be safe towards the normal cell
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Oncolytic viral therapy for gliomas: Advances in the mechanisms and approaches to delivery Virology (IF 3.7) Pub Date : 2024-02-20 A. Romanishin, A. Vasilev, E. Khasanshin, A. Evtekhov, E. Pusynin, K. Rubina, V. Kakotkin, M. Agapov, E. Semina
Glioma is a diverse category of tumors originating from glial cells encompasses various subtypes, based on the specific type of glial cells involved. The most aggressive is glioblastoma multiforme (GBM), which stands as the predominant primary malignant tumor within the central nervous system in adults. Despite the application of treatment strategy, the median survival rate for GBM patients still hovers
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Analysis of codon usage bias of exonuclease genes in invertebrate iridescent viruses Virology (IF 3.7) Pub Date : 2024-02-19 Yeşim Aktürk Dizman
Invertebrate iridescent viruses (IIVs) are double-stranded DNA viruses that belong to the family. IIVs result diseases that vary in severity from subclinical to lethal in invertebrate hosts. Codon usage bias (CUB) analysis is a versatile method for comprehending the genetic and evolutionary aspects of species. In this study, we analyzed the CUB in 10 invertebrate iridescent viruses exonuclease genes
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Investigation and characterization of rice dwarfing epidemic caused by southern rice black-streaked dwarf virus in Jiangsu in 2023 Virology (IF 3.7) Pub Date : 2024-02-18 Chenyang Li, Wenxuan Yang, Yuanming Zhang, Feng Zhu, Yanglin Qiu, Linlin Du, Feng Lin, Ying Lan, Kai Xu, Tong Zhou
During the field surveys in Jiangsu Province, China, contiguous patches of rice plants with varying degrees of dwarfing, wax-white or dark brown enations at the base of stems, and abnormal heading symptoms were observed in the fields located in Jiangning District in Nanjing City, Jurong County in Zhenjiang City, and Zhangjiagang County in Suzhou City. Through molecular analyses, the presence of southern
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Effect of Enteromorpha polysaccharides on gut-lung axis in mice infected with H5N1 influenza virus Virology (IF 3.7) Pub Date : 2024-02-17 Yanyi Huang, Peiyang Zhang, Shuyi Han, Bin Hu, Qingxun Zhang, Hongxuan He
Enteromorpha polysaccharides (EPPs) have been reported to have antiviral and anti-inflammatory properties. To explore the effect of EPPs on H5N1-infected mice, mice were pretreated with EPPs before being infected with the H5N1 influenza virus intranasally. H5N1 infection resulted in body-weight loss, pulmonary and intestinal damage, and an imbalance of gut microbiota in mice. As a result of the inclusion
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Mucosal vaccines for viral diseases: Status and prospects Virology (IF 3.7) Pub Date : 2024-02-15 Bingjie Ma, Mengxiao Tao, Zhili Li, Quanfang Zheng, Haigang Wu, Peirong Chen
Virus-associated infectious diseases are highly detrimental to human health and animal husbandry. Among all countermeasures against infectious diseases, prophylactic vaccines, which developed through traditional or novel approaches, offer potential benefits. More recently, mucosal vaccines attract attention for their extraordinary characteristics compared to conventional parenteral vaccines, particularly
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Emerging challenges in the management of Orthotospoviruses in Indian agriculture Virology (IF 3.7) Pub Date : 2024-02-15 R.K. Mohana Pradeep, V. Rakesh, N. Boopathi, M. Siva, S. Kousalya, K. Nagendran, G. Karthikeyan
Orthotospoviruses, a genera of negative-sense ssRNA viruses transmitted by thrips, have gained significant attention in recent years due to their detrimental impact on diverse crops, causing substantial economic losses and posing threats to food security. Orthotospoviruses are characterised by a wide range of symptoms in plants, including chlorotic/necrotic spots, vein banding, and fruit deformation
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Development and application of sugarcane streak mosaic virus vectors Virology (IF 3.7) Pub Date : 2024-02-15 Yuteng Yin, Duan Wang, Hongwei Wang, Yanwei Sun, Cece Yin, Jie Li, Jian Ye
Sugarcane streak mosaic virus (SCSMV) is one of the major pathogens of sugarcane in the world. Molecular studies and disease management of SCSMV are hindered by the lack of efficient infectious clones. In this study, we successfully constructed infiltration based infectious clone of SCSMV with different variants. Infectious clones of wild type SCSMV could efficiently infect and sugarcane plants resulting
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Identification of several African swine fever virus replication inhibitors by screening of a library of FDA-approved drugs Virology (IF 3.7) Pub Date : 2024-02-14 Tingting Li, Jun Zheng, Tao Huang, Xiao Wang, Jiangnan Li, Feng Jin, Wenjuan Wei, Xin Chen, Chuanxia Liu, Miaofei Bao, Gaihong Zhao, Li Huang, Dongming Zhao, Jianxin Chen, Zhigao Bu, Changjiang Weng
African swine fever (ASF) caused by African swine fever virus (ASFV) is a highly infectious and lethal swine disease. Currently, there is only one novel approved vaccine and no antiviral drugs for ASFV. In the study, a high-throughput screening of an FDA-approved drug library was performed to identify several drugs against ASFV infection in primary porcine alveolar macrophages. Triapine and cytarabine
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Determination of the three-dimensional structure of bacteriophage Mu(-) tail fiber and its characterization. Virology (IF 3.7) Pub Date : 2024-02-13 Yukina Mori, Eiki Yamashita, Atsushi Nakagawa, Tomoki Matsuzawa, Minoru Inagaki, Yoshifumi Aiba, Syu Tanaka, Syunya Hatori, Maeda Ayami, Shigeki Takeda
Bacteriophage Mu is a phage known to infect various species of Enterobacteria, playing a role in bacterial mutation induction and horizontal gene transfer. The phage possesses two types of tail fibers for host recognition, which enable it to expand its range of hosts. The alternate tail fibers are formed through the action of genes 49–50 or 52–51, allowing the Mu phage to recognize different surfaces
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Development of full-length infectious cDNA clones and host range identification of an echinacea strain of tobacco streak virus Virology (IF 3.7) Pub Date : 2024-02-11 Qi Liu, Menglin Li, Xuanyu Dong, Chengxiao Zuo, Zhangyao Nie, Zongying Zhang, Cheng-Gui Han, Ying Wang
Tobacco streak virus induces severe diseases on a wide range of plants and becomes an emerging threat to crop yields. However, the infectious clones of TSV remain to be developed for reverse genetics studies. Here, we obtained the full genome sequence of a TSV-CNB isolate and analyzed the phylogenetic characteristics. Subsequently, we developed the full-length infectious cDNA clones of TSV-CNB driven
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Distribution patterns and functional diversity of DNA viruses determined by ecological niches in huge river ecosystems Virology (IF 3.7) Pub Date : 2024-02-11 Kang Zhou, Hui Zhou
While the vast number of DNA and RNA viruses participate in biogeochemical cycles in natural systems, little is known about virome in river ecosystems. Here, we analyzed the DNA viral composition and its metabolic potential in the Yangtze River, including freshwater (FW) and freshwater sediments (FWS). A total of 1237 river-derived virus contigs (RVCs) were obtained following de novo assembly from
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Preliminary molecular study for DIVA trial of antigenically characterized circulating bovine herpesvirus subtype 1.1 in Egypt. Virology (IF 3.7) Pub Date : 2024-02-10 Basel A. Abukhadra, Sahar Abd El Rahman, Mohamed A. Soltan, Giuma E. Elhafi, Samah M. Mosad
Using marker vaccines to control bovine alphaherpesvirus-1 (BoHV-1) is a novel strategy for differentiation between infected and vaccinated animals (DIVA). In this study, multiplex real-time PCR targeting gD and gE genes was applied for BoHV-1 screening on 60 clinical samples from cattle with a history of vaccination, in some cases by US2-deleted marker vaccines, that were suffering from severe respiratory
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p53 suppresses the inflammatory response following respiratory syncytial virus infection by inhibiting TLR2 Virology (IF 3.7) Pub Date : 2024-02-10 Jiao Liu, Leiqiong Gao, Na Zhou, Zhenghong Jiang, Siyi Che, Yu Deng, Na Zang, Luo Ren, Xiaohong Xie, Jun Xie, Enmei Liu
—Respiratory syncytial virus (RSV) is a pivotal virus leading to acute lower respiratory tract infections in children under 5 years old. This study aimed to explore the correlation between p53 and Toll-like receptors (TLRs) post RSV infection. p53 levels exhibited a substantial decrease in nasopharyngeal aspirates (NPAs) from infants with RSV infection compared to control group. Manipulating p53 expression
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Genome analysis of a novel avian atadenovirus reveals a possible horizontal gene transfer Virology (IF 3.7) Pub Date : 2024-02-08 Weibo Zheng, Xiaopeng Teng, Tingshu Jiang, Wenli Tang, Linlin Jiang, Hongwei Zhu, Xin Yu, Guozhong Chen, Jiao Wang, Jianlong Zhang, Mingjuan Qu, Xingxiao Zhang
We report the discovery and characterization of a novel adenovirus, Zoothera dauma adenovirus (ZdAdV), from a wild bird species, (Scaly thrush). This new atadenovirus was discovered by metagenomic sequencing without virus cultivation. Analyses of the full genome sequence revealed that this new virus is a distinct member of the genus and represents a novel species. ZdAdV has a genome of 34,760 bp with
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Australian terrestrial environments harbour extensive RNA virus diversity Virology (IF 3.7) Pub Date : 2024-02-08 Sabrina Sadiq, Erin Harvey, Jonathon C.O. Mifsud, Budiman Minasny, Alex B. McBratney, Liana E. Pozza, Jackie E. Mahar, Edward C. Holmes
Australia is home to a diverse range of unique native fauna and flora. To address whether Australian ecosystems also harbour unique viruses, we performed meta-transcriptomic sequencing of 16 farmland and sediment samples taken from the east and west coasts of Australia. We identified 2460 putatively novel RNA viruses across 18 orders, the vast majority of which belonged to the microbe-associated phylum
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Meta-transcriptomic identification of groundnut RNA viruses in western Kenya and the novel detection of groundnut as a host for Cauliflower mosaic virus Virology (IF 3.7) Pub Date : 2024-02-06 Dennis Obonyo, George Ouma, Rachel Ikawa, Damaris A. Odeny
Groundnut ( L.) is the 13th most important global crop grown throughout the tropical and subtropical regions of the world. One of the major constraints to groundnut production is viruses, which are also the most economically important and most abundant pathogens among cultivated legumes. Only a few studies have reported the characterization of RNA viruses in cultivated groundnuts in western Kenya,
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Engineering of stable infectious cDNA constructs of a fluorescently tagged tomato chlorosis virus Virology (IF 3.7) Pub Date : 2024-02-06 Sun-Jung Kwon, Ye-Ji Lee, Young-Eun Cho, Hee-Seong Byun, Jang-Kyun Seo
Tomato chlorosis virus (ToCV) is an emerging pathogen that cause severe yellow leaf disorder syndrome in tomato plants. In this study, we aimed to generate a recombinant ToCV tagged with green fluorescent protein (GFP) to enable real-time monitoring of viral infection in living plants. Transformation of the full-length cDNA construct of ToCV RNA1 into resulted in instability issues, which were successfully
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Proprotein convertase cleavage of Ictalurid herpesvirus 1 spike-like protein ORF46 is modulated by N-glycosylation Virology (IF 3.7) Pub Date : 2024-02-06 Fei Yu, Jiehua Xu, Hongxun Chen, Siyang Song, Chunlan Nie, Kai Hao, Zhe Zhao
Viral spike proteins undergo a special maturation process that enables host cell receptor recognition, membrane fusion, and viral entry, facilitating effective virus infection. Here, we investigated the protease cleavage features of ORF46, a spike-like protein in 1 (IcHV-1) sharing similarity with spikes of Nidovirales members. We noted that during cleavage, full-length ORF46 is cleaved into ∼55-kDa
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Isolation and characterization of genotype 4 Eurasian avian-like H1N1 influenza virus in pigs suffering from pneumonia Virology (IF 3.7) Pub Date : 2024-02-02 Jizhe Yang, Riguo Lan, Haoyu Chang, Han Li, Haili Yu, Qi Tong, Jinhua Liu, Honglei Sun
Swine influenza viruses pose ongoing threat to pork industry throughout the world. In 2023, fattening pigs from a swine farm in Inner Mongolia of China experienced influenza-like symptoms. Co-infection of influenza A virus with was diagnosed in lung tissues of diseased pigs and a genotype 4 (G4) Eurasian avian-like (EA) H1N1 virus was isolated, which was named as A/swine/Neimenggu/0326/2023. We demonstrated
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A cell-adapted SARS-CoV-2 mutant, showing a deletion in the spike protein spanning the furin cleavage site, has reduced virulence at the lung level in K18-hACE2 mice. Virology (IF 3.7) Pub Date : 2024-01-28 Fabrizia Valleriani, Chiara Di Pancrazio, Massimo Spedicato, Giovanni Di Teodoro, Daniela Malatesta, Tetyana Petrova, Francesca Profeta, Maria Loredana Colaianni, Shadia Berjaoui, Ilaria Puglia, Marialuigia Caporale, Emanuela Rossi, Maurilia Marcacci, Mirella Luciani, Flavio Sacchini, Ottavio Portanti, Francesco Bencivenga, Nicola Decaro, Francesco Bonfante, Alessio Lorusso
Here we investigated the virulence properties of a unique cell-adapted SARS-CoV-2 mutant showing a ten-amino acid deletion encompassing the furin cleavage site of the spike protein (Δ680SPRAARSVAS689; Δ680-689-B.1) in comparison to its parental strain (wt-B.1) and two Delta variants (AY.122 and AY.21). After IN inoculation, transgenic K18-hACE2 mice were monitored for 14 days for weight change, lethality
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Application of nanoparticles for management of plant viral pathogen: Current status and future prospects Virology (IF 3.7) Pub Date : 2024-01-26 Ashish Warghane, Rashmi Saini, Manju Shri, Isha Andankar, Dilip Kumar Ghosh, Balu Ananda Chopade
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Comparative analysis and prediction of avian influenza in Shangrao city, China from 2016 to 2022 Virology (IF 3.7) Pub Date : 2024-01-24 Kang Fang, Wentao Song, Yanyan Zhang, Yiyang Zheng, Chen You, Jianhai Hu, Li Liu, Lei Feng, Zeyu Zhao, Yunkang Zhao, Jiayi Wang, Xiaolan Wang, Lin Zhu, Tianmu Chen
The aim of this study was to investigate the effects of vaccination, COVID-19 pandemic and migration of migratory birds on the avian influenza positivity rate in Shangrao City and to predict the future avian influenza positivity rate. Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to detect nucleic acids of avian influenza A viruses. 1795 samples were collected between
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Effect of chemical and physical agents on monkeypox virus infectivity and downstream research applications Virology (IF 3.7) Pub Date : 2024-01-16 Davide Mariotti, Aurora Bettini, Silvia Meschi, Stefania Notari, Massimo Francalancia, Eleonora Tartaglia, Daniele Lapa, Eliana Specchiarello, Enrico Girardi, Giulia Matusali, Fabrizio Maggi
The 2022 global spread of Monkeypox Virus (MPXV) underlined the need to investigate safe-handling procedures of clinical and research samples. Here we evaluated the efficiency in reducing MPXV infectious titer of Triton X-100 (0.1 and 0.2%), UV-C irradiation (15 or 30 min), and heat (56 °C 30 min or 70 °C 5 min). The treatment of MPXV at 70 °C resulted in the strongest decrease of MPXV infectious titer
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A neonatal piglet model reveals interactions between nasal microbiota and influenza A virus pathogenesis Virology (IF 3.7) Pub Date : 2024-01-20 Fangfeng Yuan, Lufan Yang, Shih-Hsuan Hsiao, Nicole L. Herndon, Christopher A. Gaulke, Ying Fang
While vaccination and therapeutics for prevention/treatment of influenza are available, new strategies are needed to combat influenza disease in susceptible populations, particularly young children and newborns. Host associated microbiota play an important role in modulating the virulence of numerous pathogens, including the influenza A virus. In this study, we examined microbiome-influenza interactions
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Development of a monoclonal antibody specifically recognizing a linear epitope on the capsid protein of the emerging Group III Getah virus Virology (IF 3.7) Pub Date : 2024-01-11 Lingshan Zhou, Tongwei Ren, Muyang Liu, Xianglin Min, Liping Zhang, Yifeng Qin, Kang Ouyang, Ying Chen, Weijian Huang, Zuzhang Wei
Getah virus (GETV) is an emerging mosquito-borne alphavirus that can infect horses, pigs and other animals. Given the public health threat posed by GETV, research on its pathogenesis, diagnosis and prevention is urgently needed. In the current study, prokaryotic expression systems were used to express the capsid protein of GETV. This protein was then used to immunize BALB/c mice in order to generate
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Monkeypox: Can we count on the current smallpox immunization? Virology (IF 3.7) Pub Date : 2024-01-14 Fengwei Zhang, Zili Chai, Xia Wang, Zehan Zhang, Zilong Yang, Wenting Liu, Hongguang Ren, Yuan Jin, Junjie Yue
Two vaccines ACAM 2000 and JYNNEOS have obtained approval from the Food and Drug Administration as preventive measures against monkeypox, contributing significantly to the management of the monkeypox epidemic. Nonetheless, research has demonstrated that smallpox vaccination offers approximately 88.8% protection against monkeypox, while immunization with these vaccines generates relatively low levels
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A GFP-expressing minigenome of a chrysovirus replicating in fungi Virology (IF 3.7) Pub Date : 2024-01-09 Robert Domènech-Eres, Mareike Jaeckel, Birgit Hadeler, Tim Lienemann, Tobias Lutz, Cornelia Heinze
The Fusarium graminearum virus China 9 (FgV-ch9) is a member of the genus Betachrysovirus in the Chrysoviridae family and causes hypovirulence in its host, Fusarium graminearum, the causal agent of Fusarium head blight. Although insights into viral biology of FgV-ch9 have expanded in recent years, questions regarding the function of virus-encoded proteins, cis-acting elements, and virus transmission
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Overexpression of human ACE2 protein in mouse fibroblasts stably transfected with the intact ACE2 gene Virology (IF 3.7) Pub Date : 2024-01-13 Feiyue Teng, Oleg Gang, Paul Freimuth
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Virome profiling of fig wasps (Ceratosolen spp.) reveals virus diversity spanning four realms Virology (IF 3.7) Pub Date : 2024-01-10 Bishwo N. Adhikari, Adrian C. Paskey, Kenneth G. Frey, Andrew J. Bennett, Kyle A. Long, Jens H. Kuhn, Theron Hamilton, Lindsay Glang, Regina Z. Cer, Tony L. Goldberg, Kimberly A. Bishop-Lilly
We investigated the virome of agaonid fig wasps (Ceratosolen spp.) inside syconia (“fruits”) of various Ficus trees fed upon by frugivores such as pteropodid bats in Sub-Saharan Africa. This virome includes representatives of viral families spanning four realms and includes near-complete genome sequences of three novel viruses and fragments of five additional potentially novel viruses evolutionarily
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Integration of Rubus yellow net virus in the raspberry genome: A story centuries in the making Virology (IF 3.7) Pub Date : 2024-01-09 Thien Ho, Janet C. Broome, Jason P. Buhler, Wendy O'Donovan, Tongyan Tian, Alfredo Diaz-Lara, Robert R. Martin, Ioannis E. Tzanetakis
Rubus yellow net virus (RYNV) belongs to genus Badnavirus. Badnaviruses are found in plants as endogenous, inactive or activatable sequences, and/or in episomal (infectious and active) forms. To assess the state of RYNV in Rubus germplasm, we sequenced the genomes of various cultivars and mined eight raspberry whole genome datasets. Bioinformatics analysis revealed the presence of a diverse array of
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Global phylogenetic analysis of soybean dwarf virus isolates and their associations with aphid vectors and severe disease in soybeans. Virology (IF 3.7) Pub Date : 2024-01-06 Andrew L. Stone, Vernon D. Damsteegt, Oney P. Smith, Lucy R. Stewart
Soybean dwarf virus (SbDV) was first described in Japan as an agent of severe soybean disease transmitted by the foxglove aphid, Aulacorthum solani, with separable yellowing (Y) and dwarfing (D) strains. SbDV of both Y and D genotypes were later documented in other countries. For three decades, SbDV isolates were assessed to evaluate risk to U.S. soybean production. U.S. SbDV isolates were transmitted
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The leader RNA of SARS-CoV-2 sequesters polypyrimidine tract binding protein (PTBP1) and influences pre-mRNA splicing in infected cells Virology (IF 3.7) Pub Date : 2024-01-06 Noelia H. Altina, David G. Maranon, John R. Anderson, Meghan K. Donaldson, Suad Elmegerhi, Laura A. St Clair, Rushika Perera, Brian J. Geiss, Jeffrey Wilusz
The large amount of viral RNA produced during infections has the potential to interact with and effectively sequester cellular RNA binding proteins, thereby influencing aspects of post-transcriptional gene regulation in the infected cell. Here we demonstrate that the abundant 5’ leader RNA region of SARS-CoV-2 viral RNAs can interact with the cellular polypyrimidine tract binding protein (PTBP1). Interestingly
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Emerging concerns of blood-brain barrier dysfunction caused by neurotropic enteroviral infections Virology (IF 3.7) Pub Date : 2024-01-06 Shujie Han, Wangquan Ji, Guangcai Duan, Shuaiyin Chen, Haiyan Yang, Yuefei Jin
Enteroviruses (EVs), comprise a genus in the Picornaviridae family, which have been shown to be neurotropic and can cause various neurological disorders or long-term neurological condition, placing a huge burden on society and families. The blood-brain barrier (BBB) is a protective barrier that prevents dangerous substances from entering the central nervous system (CNS). Recently, numerous EVs have
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Efficient generation of recombinant eggplant mottled dwarf virus and expression of foreign proteins in solanaceous hosts Virology (IF 3.7) Pub Date : 2024-01-06 Shuo Wang, Binhuan Chen, Shuang Ni, Yan Liang, Zhenghe Li
Reverse genetics systems have only been successfully developed for a few plant rhabdoviruses. Additional systems are needed for molecular virology studies of these diverse viruses and development of viral vectors for biotechnological applications. Eggplant mottled dwarf virus (EMDV) is responsible for significant agricultural losses in various crops throughout the Mediterranean region and the Middle
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Cucumber mosaic virus impairs the physiological homeostasis of Panax notoginseng and induces saponin-mediated resistance Virology (IF 3.7) Pub Date : 2024-01-05 Yongchao Cheng, Tianrui Zheng, Daoyong Yang, Qiding Peng, Jiahong Dong, Dehui Xi
As an important medicinal plant, Panax notoginseng often suffers from various abiotic and biotic stresses during its growth, such as drought, heavy metals, fungi, bacteria and viruses. In this study, the symptom and physiological parameters of cucumber mosaic virus (CMV)-infected P. notoginseng were analyzed and the RNA-seq was performed. The results showed that CMV infection affected the photosynthesis
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Evidence that an interaction between the respiratory syncytial virus F and G proteins at the distal ends of virus filaments mediates efficient multiple cycle infection. Virology (IF 3.7) Pub Date : 2024-01-05 Tra Nguyen Huong, Zhi Qi Lee, Soak Kuan Lai, Hsin Yee Lee, Boon Huan Tan, Richard J. Sugrue
Evidence for a stable interaction between the respiratory syncytial virus (RSV) F and G proteins on the surface of virus filaments was provided using antibody immunoprecipitation studies on purified RSV particles, and by the in situ analysis on the surface of RSV-infected cells using the proximity ligation assay. Imaging of the F and G protein distribution on virus filaments suggested that this protein
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In vitro characterization of cell-fusing agent virus DNA forms in Aedes aegypti mosquitoes Virology (IF 3.7) Pub Date : 2024-01-03 Uddin Mohammad Mosleh, Yasutsugu Suzuki, Jerica Isabel L. Reyes, Kozo Watanabe
How non-retroviral endogenous viral elements (EVEs) are established is a long-standing question. Viral DNA (vDNA) forms of RNA viruses are likely to be EVE precursors. Cell-fusing agent virus (CFAV) is a major insect-specific virus (ISV) in the Aedes aegypti mosquitoes and one of the few existing non-retroviral RNA viruses found as EVEs. We characterized CFAV-derived vDNA in the cell line to understand
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Unusual outbreaks of curly top disease in processing tomato fields in northern California in 2021 and 2022 were caused by a rare strain of beet curly top virus and facilitated by extreme weather events Virology (IF 3.7) Pub Date : 2024-01-04 Tomas A. Melgarejo, Margaret K. Cespedes, Li-Fang Chen, Thomas Turini, Patricia A. Lazicki, Amber Vinchesi-Vahl, Robert L. Gilbertson
In the western United States, curly top disease (CTD) is caused by beet curly top virus (BCTV). In California, CTD causes economic loss to processing tomato production in central and southern areas but, historically, not in the north. Here, we document unusual CTD outbreaks in processing tomato fields in the northern production area in 2021 and 2022, and show that these were caused by the rare spinach
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Epidermal growth factor receptor-dependent stimulation of differentiation by human papillomavirus type 16 E5 Virology (IF 3.7) Pub Date : 2023-12-12 Jessica Trammel, Oluwamuyiwa Amusan, Allison Hultgren, Gaurav Raikhy, Jason M. Bodily
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Efficacy and tolerability of an mRNA vaccine expressing gB and pp38 antigens of Marek’s disease virus in chickens Virology (IF 3.7) Pub Date : 2023-12-16 Fatemeh Fazel, Ayumi Matsuyama-Kato, Mohammadali Alizadeh, Nitish Boodhoo, Shayan Sharif
Marek's disease is a contagious proliferative disease of chickens caused by an alphaherpesvirus called Marek's disease virus. A bivalent mRNA vaccine encoding MDV's glycoprotein-B and phosphoprotein-38 antigens was synthesized and encapsulated in lipid nanoparticles. Tumor incidence, lesion score, organ weight indices, MDV genome load and cytokine expression were used to evaluate protection and immunostimulatory
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Unveiling tetrahydroquinolines as promising BVDV entry inhibitors: Targeting the envelope protein Virology (IF 3.7) Pub Date : 2023-12-15 Emilse S. Leal, María J. Pascual, Natalia S. Adler, Nicolás Arrupe, Fernando Merwaiss, Luciana Giordano, Daniela Fidalgo, Diego Álvarez, Mariela Bollini
Bovine viral diarrhea virus (BVDV) is known to cause financial losses and decreased productivity in the cattle industry worldwide. Currently, there are no available antiviral treatments for effectively controlling BVDV infections in laboratories or farms. The BVDV envelope protein (E2) mediates receptor recognition on the cell surface and is required for fusion of virus and cell membranes after the
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Action mechanisms and characteristics of miRNAs to regulate virus replication Virology (IF 3.7) Pub Date : 2023-12-09 Jia Yao, Yating Zhu, Genrong Zhang, Xianfeng Zhou, Hongcai Shang, Longxue Li, Tielong Xu
MicroRNAs (miRNAs) have the potential to be explored as antiviral products. It is known that miRNAs have different kinds of target mRNAs and different target sites in mRNAs, and that the action-modes of miRNAs at different target sites may be different. But there is no evidence demonstrating the significance of the differences for the regulation of viruses by miRNAs, which might be crucial for the
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Bleomycin promotes rAAV2 transduction via DNA-PKcs/Artemis-mediated DNA break repair pathways Virology (IF 3.7) Pub Date : 2023-12-09 Xiaoping Huang, Xiao Wang, Yaqi Sun, Ling Li, Anna Li, Wentao Xu, Xiaolan Xie, Yong Diao
Because it is safe and has a simple genome, recombinant adeno-associated virus (rAAV) is an extremely appealing vector for delivery in in vivo gene therapy. However, its low transduction efficiency for some cells, limits its further application in the field of gene therapy. Bleomycin is a chemotherapeutic agent approved by the FDA whose effect on rAAV transduction has not been studied. In this study
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The p30 protein of the African swine fever virus behaves as an RNase Virology (IF 3.7) Pub Date : 2023-12-09 Satyendu Nandy, Nilave Ranjan Bora, Shubham Gaurav, Sachin Kumar
The African Swine Fever Virus (ASFV) is responsible for causing African Swine Fever (ASF), a severe contagious disease characterized by hemorrhagic symptoms. The p30 protein of ASFV is the most abundantly expressed viral protein. It is reported to be antigenic and has recognized phosphorylation, glycosylation, and membrane attachment sites, which also shows that the C-terminal region of p30 is more