-
Challenges and pragmatic solutions for assessing the reliability of HIV-1 viral load monitoring in resource-constrained settings. Intervirology (IF 4.6) Pub Date : 2023-11-26 John Paul Demosthenes,Ben Chirag Ghale,Diviya Alex,Veena Vadhini Ramalingam,Gnanadurai John Fletcher,Priya Abraham,Rajesh Kannangai
INTRODUCTION HIV-1 RNA detection is the most reliable method for monitoring treatment response among people living with HIV (PLHIV). Effective quality control measures that include internal quality control (IQC) are challenging in resource-constrained settings. METHODS We ascertained the utility of the assay low positive control (LPC) as an effective internal quality control (IQC) to monitor the reliability
-
Comparison of a Multiplex Real-Time PCR technique with Oxford Nanopore Technologies Next-Generation-Sequencing for identification of SARS-CoV-2 variants of concern. Intervirology (IF 4.6) Pub Date : 2023-10-09 Zahra Ahmadi,Ali Maleki,Sana Eybpoosh,Zahra Fereydouni,Mahsa Tavakoli,Setareh Kashanian,Laya Asadi,Amir Hesam Nemati,Mostafa Salehi-Vaziri
INTRODUCTION The rapid emergence of SARS-CoV-2 variants and their potential to endangering the global health, has increased the demand for a fast-tracking method in comparison to the Next-Generation-Sequencing (NGS) as a gold standard assay, particularly in developing countries. This study was designed to evaluate the performance of a commercial multiplex Real-Time PCR technique (GA SARS-CoV-2 OneStep
-
Seroprevalence of Enterovirus D68 infection among humans: A systematic review and meta-analysis. Intervirology (IF 4.6) Pub Date : 2023-10-04 Yingying Shi,Qinqin Ran,Xiaochen Wang,Lu Shi
INTRODUCTION Human enterovirus D68 (EV-D68), which belongs to enteroviruses of the small RNA family, is a type of enterovirus that can cause acute respiratory tract infection and central nervous system diseases. This study systematically analysed and summarized EV-D68 antibody studies in databases and identified the seropositivity rates of different regions, ages, and sexes. METHODS Meta-analysis was
-
New potential miRNA biomarkers in HIV Elite Controllers, HIV infections and Co-Infections with HBV or HCV. Intervirology (IF 4.6) Pub Date : 2023-09-12 Bashdar Mahmud Hussen,Majid Noori,Babak Sayad,Maryam Ebadi Fard Azar,Javid Sadri Nahand,Mobina Bayat,Farhad Babaei,Romina Karampour,Farah Bokharaei-Salim,Hamed Mirzaei,Mohsen Moghoofei,Hossein Bannazadeh Baghi
INTRODUCTION This research aimed to evaluate the specific miRNA including miR-17-5p, miRN-140-3p miR-191-5p, miR-200c-3p, and miR-N367 and cellular factors (p21, SDF-1, XCL1, CCL-2 and IL-2) in controlling replication of HIV in ECs. METHODS The expression of miRNAs was assessed between healthy control groups and patient groups including ART-naïve HIV, HIV ART, ECs, and co-infection (HIV-HBV and HIV-HCV)
-
Herpes Simplex Virus Type 1 MicroRNAs: An Update. Intervirology (IF 4.6) Pub Date : 2023-06-07 Yongzhong Duan,Le Sun,Qihan Li
BACKGROUND Herpes simplex virus type 1 (HSV-1), an important human pathogen, is capable of latent infection in neurons and productive (lytic) infection in other tissue cells in vivo. Once infected with HSV-1, the immune system of the organism cannot eliminate the virus and carry it lifelong. HSV-1 possesses approximately 150 kb of double-stranded linear genomic DNA and can encode at least 70 proteins
-
Impact of Age and Clinico-Biochemical Parameters on Clinical severity of SARS-CoV-2 Infection. Intervirology (IF 4.6) Pub Date : 2023-06-01 Shariq Ahmad Wani,Babar Gulzar,Mosin Saleem Khan,Sabhiya Majid,Irfan Ahmad Bhat
INTRODUCTION The surge in novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection leading to COrona VIrus Disease-2019 (COVID-19) has overwhelmed the health system. To help health-care workers and policy makers prioritize treatment and to decrease the burden on health systems caused by COVID-19, clinical severity along with various clinico-biochemical parameters was evaluated by
-
Hepatitis C Virus Genotype 5 Variability in Treatment-Naïve Patients in South Africa. Intervirology (IF 4.6) Pub Date : 2023-05-09 Tshegofatso K Maunye,Maemu P Gededzha,Jason T Blackard,Johnny N Rakgole,Selokela G Selabe
INTRODUCTION Hepatitis C virus (HCV) genotype 5 was originally identified in South Africa, where it represents 35-60% of all HCV infections. There are limited data on resistance-associated variants (RAVs) in South Africa. Thus, we investigated variability within the NS3/NS4A, NS5A, and NS5B genes of treatment-naïve individuals with HCV genotype 5 infection at the Dr. George Mukhari Academic Hospital
-
Evaluation of MicroRNAs expression pattern (miR-28, miR-181a, miR-34a, and miR-31) in Patients with COVID-19 Admitted to ICU, and Diabetic COVID-19 patients Intervirology (IF 4.6) Pub Date : 2023-03-07 AliReza Khatami, Mohammad Taghizadieh, Javid Sadri Nahand, Mohammad Karimzadeh, Seyed Jalal Kiani, Khadijeh Khanaliha, Saeed Kalantari, Sara Chavoshpour, Hamed Mirzaei, Tahereh Donyavi, Farah Bokharaei-Salim
Introduction: MicroRNAs, or miRNAs, with regulatory performance in inflammatory responses and infection are the prevalent manifestations of severe Coronavirus disease (COVID-19). This study aimed to evaluate whether PBMC miRNAs are diagnostic biomarkers to screen the ICU COVID-19 and diabetic-COVID-19 subjects. Methods: Candidate miRNAs were selected through previous studies, and then the PBMC levels
-
Clinical value of serum miR-106a in the diagnosis and prognosis of human papillomavirus-positive cervical cancer Intervirology (IF 4.6) Pub Date : 2023-01-16
Introduction: Cervical cancer (CC) is a prevailing malignant tumor in women, mainly caused by human papillomavirus (HPV) infection. This study investigated miR-106a expression in the serum of HPV-positive CC patients and estimated its value in diagnosis and prognosis. Methods: We enrolled 120 CC patients as study subjects, with another 80 healthy women as controls. Clinical baseline data and clinicopathological
-
Broad antiviral spectrum of glycyrrhizic acid for human and veterinary medicine: reality or fiction? Intervirology (IF 4.6) Pub Date : 2022-12-01 Gilberto Garcia-Salazar, Zaida Urbán-Morlán, Susana Mendoza-Elvira, David Quintanar-Guerrero, Sandra Mendoza
Background: Emerging virus infections provoke health problems in people and animals, which generate social, and economic issues worldwide. This has spurred the search for new pharmacological strategies to confront them. Summary: The purpose of this review is to draw the reader's attention to pharmacological evaluations of glycyrrhizic acid (GA) and its analogs on the broad range of viruses known in
-
PERV induces CXCL10 in human monocytes and monocyte-derived primary cells Intervirology (IF 4.6) Pub Date : 2022-11-16 Hussein Al-Shehabi, Norbert Bannert
Introduction: Pigs are suitable donor species for xenotransplantation and biological materials from these animals are used for this purpose for many years. A major risk of xenotransplantation is a zoonosis by transspecies transmission of animal viruses. In this regard Porcine Endogenous Retroviruses (PERVs) are of paramount importance because some of them are able to infect human cells and could induce
-
-
Rotavirus quantification in children with acute gastroenteritis Intervirology (IF 4.6) Pub Date : 2022-10-14 Ilaria Galliano, Valentina Daprà, Maddalena Dini, Martina Gavatorta, Allegra Sardo, Giada Lo Curcio, Massimiliano Bergallo
Introduction: Most intractable diarrheas are treated with antibiotics, irrespective of the causative agent. This study aims to quantified Rotavirus with taqman real-time PCR in fecal samples of children with acute gastroenteritis in accordance with the program of reduction of drug-resistance and use of antibiotics. Methods: A total of 190 fecal specimens were collected from under-five-year-old children
-
Relationship of noncoding RNA and swine viruses Intervirology (IF 4.6) Pub Date : 2022-10-11 Manxin Fang, Wei Hu, Zhibang Deng
Swine viruses are well known as a threat to the pig industry. Many signaling pathways and a number of proteins were discovered to participate in the immune responses to swine viruses. Noncoding RNAs (ncRNAs), comprising a different set of transcripts including housekeeping RNAs (for example, rRNAs and tRNAs) and regulatory RNAs (small RNAs and long non-coding RNAs), recently have been described as
-
Disinfection effect of hexadecyl pyridinium chloride on SARS-CoV-2 in vitro Intervirology (IF 4.6) Pub Date : 2022-09-14 Ke-da Chen, Fei-ke Ma, Qing-jing Wang, Ying Wang, Xin-yi Zhuang, Xu-ning Zhang, Hai-yan Mao, Yan-jun Zhang
The novel coronavirus (COVID-19 or 2019-nCoV) is a respiratory virus that can exist in the mouth and saliva of patients and spreads through aerosol dispersion. Therefore, stomatological hospitals and departments have become high-infection-risk environments. Accordingly, oral disinfectants that can effectively inactivate the virus have become a highly active area of research. Hexadecyl pyridinium chloride
-
Effects of Resveratrol on Hepatitis B Virus Replication: In vitro and in vivo Experiments Intervirology (IF 4.6) Pub Date : 2022-09-09 Peipei Pan, Jiaohui Li, Wei Lin, Guangyan Long
Introduction: Hepatitis B virus (HBV) infection is a disease with high incidence and lack of effective treatment. In this study, we further explored the mechanism of resveratrol (RVT) in the inhibition of HBV replication. The effects of RVT on HBV replication were verified using in vitro and in vivo experiments. Methods: HepG2 and HepG2.2.15 cell lines were cultured in vitro, and different concentrations
-
Molecular detection and genotyping of Human Cytomegalovirus in kidney transplant recipients under ganciclovir prophylaxis in Iran Intervirology (IF 4.6) Pub Date : 2022-07-20 Farzane Behnezhad, Najmeh Parhizgari, Nazanin Zahra Shafiei-Jandaghi, Jila Yavarian, Talat Mokhtari-Azad
Abstract Introduction: Human Cytomegalovirus (HCMV) infection is one of the most common viral complications in kidney transplant recipients. Although there are effective treatments strategies for the HCMV infection, this infection is still one of the causes of kidney transplant rejection. Methods: A total of 246 kidney transplant recipients participated in this cross-sectional study. Viral DNA was
-
The relationship between HPV prevalence and DNA damage in cervical cancer population in Gansu province, China. Intervirology (IF 4.6) Pub Date : 2022-07-12 Zhong Guo, Tianyuan Zhu, Yunsong Zhou, Tianbin Si, Qiang Wang, Hongmei Qu, Chenjing Wang, Jin Zhao
Objectives: To assess the possible reason of high incidence and mortality of cervical cancer in Longnan, China. Materials and Methods: 147 and 124 invasive squamous cell carcinoma (SCC) samples from Longnan and different cities and districts of Gansu province were collected in the present study. All the samples were obtained from patients who underwent biopsies with colposcopy or advanced operations
-
Identification and Verification of Ubiquitin D as a gene associated with HCV-induced HCC Intervirology (IF 4.6) Pub Date : 2022-06-21 Huanqin Li, Shumin Liu, Yun Lin, Xiongfei Shi, Na Du, Jing Yao, Ruiyang Liu, Yan Du, Kai Yang
Introduction: Accumulated studies have suggested that hepatitis C virus (HCV) infection is one of the leading causes for hepatocellular carcinoma (HCC). However, the mechanisms underlying the effect of HCV on the occurrence of HCC is still poorly understood. Methods: HCV infection datasets (GSE82177 and GSE17856) and HCC datasets (TCGA-LIHC (The Cancer Genome Atlas Liver Hepatocellular Carcinoma) and
-
Incidence of HPVs, EBV and MMTV-like Virus in Breast Cancer in Qatar Intervirology (IF 4.6) Pub Date : 2022-05-31 Ishita Gupta, Reem Al-Sarraf, Hanan Farghaly, Semir Vranic, Ali A. Sultan, Hamda Al-Thawadi, Ala-Eddin Al Moustafa, Halema F. Al-Farsi
Introduction: Human papillomaviruses (HPVs), Epstein-Barr virus (EBV), and mouse mammary tumor virus-like virus (MMTV-like virus) can be present and contribute to breast cancer development and progression. However, the role of these oncoviruses and their crosstalk in breast cancer is still unclear. Methods: We explored the co-presence of high-risk HPVs, EBV, and MMTV-like virus in 74 breast cancer
-
SARS-COV-2 triggers the development of class I and class II HLA antibodies in recovered convalescent plasma donors Intervirology (IF 4.6) Pub Date : 2022-04-04 Ashraf Dada, Khalid Elhassan, Rayan Mohammed Bawayan, Ghadeer Albishi, Lama Hefni, Sawsan Bassi, Turki Sobahy, Edward Cupler, Nabeela AlBaz, Ghassan Wali, Basem Alraddadi, Abeer N. Alshukairi
Various studies have shown that SARS-CoV-2 is a highly immunogenic virus. It is known that different types of immunogenic viral pathogens could trigger the formation of HLA antibodies. Therefore, there is a concern that the SARS-CoV-2 could also induce the development of HLA antibodies in volunteers, who donate convalescent plasma after their recovery from COVID-19. HLA antibodies have been identified
-
Latest insights on the diagnostic approaches and treatment strategies of COVID-19 Intervirology (IF 4.6) Pub Date : 2022-03-04 Sarah S. Hassanein, Muhammed R. Sharaby, Noran M. Tawfik, Suzan A. Rashed, Mohamed Adel, Ayat Fayez, Heba Mansour, Haitham M. Amer
Background: COVID-19 has emerged as the most serious pandemic in the 21st century to date. COVID-19 patients may develop various disease symptoms that hinder the accurate clinical diagnosis. Summary: Routine diagnosis of COVID-19 requires complementary investigations, including computed tomography, immunological assays, and molecular assays like real-time RT-PCR, loop-mediated isothermal amplification
-
Development of RNA-based assay for rapid detection of SARS-CoV-2 in clinical samples Intervirology (IF 4.6) Pub Date : 2022-02-22 Vinod Kumar, Suman Mishra, Rajni Sharma, Jyotsna Agarwal, Ujjala Ghoshal, Tripti Khanna, Lokendra K. Sharma, Santosh Kumar Verma, Prabhakar Mishra, Swasti Tiwari
Introduction: The ongoing spread of pandemic coronavirus disease (COVID-19) caused by Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) is of growing concern. Rapid diagnosis and management of SARS-CoV-2 is crucial for controlling the outbreak in the community. Here we report the development of a first rapid-colorimetric assay capable of detecting SARS-CoV-2 in the human nasopharyngeal RNA
-
Evaluation of IL-1β and IL-6 expression following EBNA-1 and BRLF-1 peptide treatment in Epstein Barr virus-positive multiple sclerosis patients Intervirology (IF 4.6) Pub Date : 2022-02-14 Roya Kianfar, Mehrdad Ravanshad, Mohammad Adel Ghiass, Nastaran Rafiee, Ali Shayeghpour, Ali Maleki
Introduction: Epstein-barr virus (EBV/HHV-4) has been implicated in the pathogenesis of Multiple sclerosis (MS). This study was conducted to investigate the levels of pro-inflammatory cytokines IL-1β and IL-6 in healthy Epstein-Barr virus (EBV) carriers and MS patients with prior EBV infection in response to treatment with Epstein–Barr virus nuclear antigen 1 (EBNA-1) and Replication and transcription
-
Low risk of serological cross-reactivity between the dengue virus and SARS-CoV-2 IgG antibodies using advanced detection assays Intervirology (IF 4.6) Pub Date : 2022-02-08 Farah M. Shurrab, Duaa W. Al-Sadeq, Fatima Humaira Amanullah, Enas S. Al-Absi, Hamda Qotba, Hadi M. Yassine, Laith J. Abu-Raddad, Gheyath K. Nasrallah
Several studies have reported serological cross-reactivity of the immune responses between SARS-CoV-2 and DENV. Most of the available studies are based on the point of care (POC) rapid testing kits. However, some rapid test kits have low specificity and can generate false positives. Hence, we aimed to investigate the potential serological cross-reactivity between SARS-CoV-2 and DENV IgG antibodies
-
Rag2 Deficiency Enhances Susceptibility to Systemic Mouse Adenovirus Type 1 Infection Intervirology (IF 4.6) Pub Date : 2021-11-04 Han-Kyul Lee, Sun-Min Seo, Jun-Young Kim, Han-Woong Kim, Eui-Suk Jeong, Yang-Kyu Choi
Introduction: Recombination-activating gene 1 (Rag1) and Rag2, which are essential in V(D)J recombination, play a crucial role in B and T cell maturation. Method: We investigated the effects of Rag2 deficiency in clustered regularly interspaced short palindromic repeats/Cas9-mediated FVB-Rag2 knockout (KO) and wild-type (WT) mice infected with mouse adenovirus type 1 (MAV-1) via the intranasal route
-
Real-World Single-Center Comparison of the Safety and Efficacy of Entecavir, Tenofovir Disoproxil Fumarate, and Tenofovir Alafenamide in Patients with Chronic Hepatitis B Intervirology (IF 4.6) Pub Date : 2021-11-03 Sara Jeong, Hyun Phil Shin, Ha Il Kim
Introduction: Chronic hepatitis B (CHB) is a major cause of chronic liver diseases and tenofovir disoproxil fumarate (TDF), tenofovir alafenamide (TAF), and entecavir (ETV) are recommended as primary treatments. This study aimed to evaluate the efficacy and safety of ETV, TDF, and TAF in a real-world clinical setting. Methods: In this retrospective cohort study, a total of 363 CHB patients who were
-
Hepatitis E virus capsid as a carrier of exogenous antigens for the development of chimeric virus-like particles Intervirology (IF 4.6) Pub Date : 2021-10-25 Tianyu Lu, Nouredine Behloul, Yi Zhou, Sarra Baha, Zhenzhen Liu, Wenjuan Wei, Ruihua Shi, Jihong Meng
-
Molecular detection of parvovirus in Manchurian chipmunks (Tamias sibiricus asiaticus) captured in Korea Intervirology (IF 4.6) Pub Date : 2021-10-25 Jin Il Kim, Kwangsook Park, Hyunho Shin, Soo Min Choi, Ki-Joon Song
Cross-species transmission of viral diseases alarms our global community for its potential of novel pandemic events. Of various viral pathogens noted recently, parvoviruses have posed public health threats not only to humans but to wild animals. To investigate the prevalence of parvoviruses in wild Manchurian chipmunks, here we detected genetic fragments of the nonstructural protein of parvovirus by
-
Acknowledgement to Reviewers Intervirology (IF 4.6) Pub Date : 2021-10-20
Intervirology 2021;64:215–216
-
The Features of Pathobiology and Clinical translation of Approved treatments for Coronavirus Disease 2019 (COVID-19) Intervirology (IF 4.6) Pub Date : 2021-10-19 Hamidreza Abbasi, Hadi Razavi Nikoo, Ali Fallah, Azadeh Mohammad-Hasani, Abasalt Hosseinzadeh Colagar, Ayyoob Khosravi
Background: The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is currently the most important etiological agent of acute respiratory distress syndrome (ARDS) with millions of infections and deaths in the last two years worldwide. Several reasons and parameters are responsible for the difficult management of coronavirus disease-2019 (COVID-19) patients; the first is virus behavioral factors
-
Seroprevalence, genotyping and monitoring of hepatitis C viral loads in patients on antivirals in Burkina Faso Intervirology (IF 4.6) Pub Date : 2021-09-28 Edwige T. Yelemkoure, Albert T. Yonli, Hermann K. Sombié, Issoufou Tao, Abdou Azaque Zouré, Abdoul Karim Ouattara, Abel P. Sorgho, Arsène W. Zongo, Moctar T.A. Zeba, Isabelle T. Kiendrebeogo, Prosper Bado, Madeleine K. Kabré, Théodora M. Zohoncon, Florencia W. Djigma, Dorcas Obiri-Yeboah, Jacques Simpore
-
Adverse Events Following AstraZeneca COVID-19 Vaccine in Saudi Arabia: A Cross-Sectional Study among Healthcare and Non-healthcare Workers Intervirology (IF 4.6) Pub Date : 2021-09-20 Ahlam A. Alghamdi, Afrah Alkazemi, Abdulrahman Alissa, Isra Alghamdi, Ghadah Alwarafi, Hadeel A. Waggas
-
Prevalence of Hepatitis B virus infection markers among patients of Ibn Sina University Hospital Center (Rabat, Morocco) Intervirology (IF 4.6) Pub Date : 2021-09-10 Mourad Feindiri, Hakima Kabbaj, Mohammed El Mzibri, Bouchra Belkadi, Najat Bouihat, Abdelkrim Filali-Maltouf, Myriam Seffar
-
Low-intensity ultrasound as a novel strategy to improve the cytotoxic effect of oncolytic reovirus on colorectal cancer model cells Intervirology (IF 4.6) Pub Date : 2021-09-10 Negar Sharifi, Hoorieh Soleimanjahi, Manijeh Mokhtari-Dizaji, Razieh Sadat Banijamali, Maliheh Elhamipour, Hesam Karimi
-
Updating on roles of HIV intrinsic factors: a review of their antiviral mechanisms and emerging functions Intervirology (IF 4.6) Pub Date : 2021-08-31 Sudarat Hadpech, Sutpirat Moonmuang, Koollawat Chupradit, Umpa Yasamut, Chatchai Tayapiwatana
-
HIV-HCV Co-Infection: Prevalence and treatment outcomes in Malaysia Intervirology (IF 4.6) Pub Date : 2021-08-26 Ali Akhtar, Samreen Fatima, Hamid Saeed, Chow Ting Soo, Amer Hayat Khan
-
Emergence of a Novel Reassortant H5N3 Avian Influenza Virus in Korean Mallard Ducks in 2018 Intervirology (IF 4.6) Pub Date : 2021-08-26 Seon-Ju Yeo, Vui Thi Hoang, Tuan Bao Duong, Ngoc Minh Nguyen, Hien Thi Tuong, Mudsser Azam, Haan Woo Sung, Hyun Park
Introduction: The avian influenza (AI) virus causes a highly contagious disease which is common in wild and domestic birds and sporadic in humans. Mutations and genetic reassortments among the 8 negative-sense RNA segments of the viral genome alter its pathogenic potential, demanding well-targeted, active surveillance for infection control. Methods: Wild duck fecal samples were collected during the
-
The Regulation of Prototype Foamy Virus 5′Long Terminal Repeats and Internal Promoter by Endogenous Transcription Factors Intervirology (IF 4.6) Pub Date : 2021-08-26 Jie Wei, Yan Sun, Ting-ting Wang, Gui Zhu, Wan-hong Liu, Xiao-hua He, Zhi Li
Background: For foamy virus, the transactivator of spumaretrovirus (Tas) could bind directly to target DNA sequences termed as Tas responsive elements and trigger the viral internal promoter (IP) and long terminal repeat (LTR) promoters. The cellular endogenous factors also play an important role in viral gene expressions. We hypothesized that except the viral transcription factor Tas, the cellular
-
The Prevalence and Genetic Characterization of Human Metapneumovirus in Bulgaria, 2016–2019 Intervirology (IF 4.6) Pub Date : 2021-07-23 Neli S. Korsun, Svetla G. Angelova, Ivelina T. Trifonova, Silvia E. Voleva, Iliana G. Grigorova, Iren S. Tzotcheva, Sirma D. Mileva, Penka I. Perenovska
Introduction: We investigated the prevalence of human metapneumovirus (hMPV) among patients with acute respiratory infections in Bulgaria, and performed genetic characterization of the F gene of these strains. Methods: Nasopharyngeal swabs collected from patients of a range of ages were tested by using real-time PCR for 12 respiratory viruses. The F gene was sequenced, and phylogenetic and amino acid
-
Seropositive reaction rates of 9 B cell epitopes of the SARS-CoV-2 spike protein and the relationship between the epitopes and neutralizing antibody Intervirology (IF 4.6) Pub Date : 2021-07-07 Li Zhu, Yunwen Zhang, Zhengrong Yang, Baisheng Li, Tiejian Feng, Xuan Zou, Jianfan He, Taiping He, Junling Li, Ning Liu, Wei Li, Xiaohui Wang
-
Isolation and Characterization of a Lytic Staphylococcus aureus Phage WV against Staphylococcus aureus Biofilm Intervirology (IF 4.6) Pub Date : 2021-07-06 Yaxian Jiang, Qian Xu, Liming Jiang, Rui Zheng
Background: Staphylococcus aureus is a Gram-positive, pathogenic bacterium that causes a wide range of symptoms in humans and can form biofilm, which is a multicellular community of microorganisms that attaches to nonbiological and biological surfaces. Methods: Here, we aimed to isolate and characterize an S. aureus phage and examine the bactericidal activity alone and in conjunction with streptomycin
-
Identification of Two Similar Novel HIV-1 Recombinant Forms (CRF01_AE/CRF07_BC) among Intravenous Drug Users in Guangxi, China Intervirology (IF 4.6) Pub Date : 2021-06-29 Fei Zhang, Yao Yang, Bingyu Liang, Yuan Yang, Qiuyu Wei, Peijiang Pan, Li Ye, Hao Liang
New kinds of HIV-1 circulating recombinant forms (CRFs) and unique recombinant forms (URFs) earn a great prevalence in China nowadays. In this study, we identified 2 similar URFs (2016GXNNIDU037 and 2019QZLSIDU253) both isolated from intravenous drug users (IDUs) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) revealed 2 URFs both clustered with CRF01_AE but setting up
-
Investigation of IL-2 and IFN-γ to EBV Peptides in Stimulated Whole Blood among Multiple Sclerosis Patients and Healthy Individuals Intervirology (IF 4.6) Pub Date : 2021-06-25 Nastaran Rafiee, Mehrdad Ravanshad, Bahador Asadi, Roya Kianfar, Ali Maleki
Introduction: Epstein-Barr virus (EBV), a double-stranded DNA virus, has 2 phases of lytic and latent infection in host cells. After infecting B lymphocytes, EBV becomes persistent in these cells. In healthy individuals, T lymphocytes play a key role in killing EBV-infected B cells. Statistical studies have shown that symptomatic EBV infection increases the risk of MS. Methods: This study intended
-
Glucose Homeostasis Is Dysregulated in Ducks Infected with Duck Hepatitis B Virus Intervirology (IF 4.6) Pub Date : 2021-06-24 Yanlian Tan, Jianxiang Liu, Yingjian Qin, Bin Liang, Yunyan Gu, Lilan Liang, Lili Liu, Yongming Liu, Heling Su
Introduction: The association between hepatitis B virus (HBV) infection and the development of diabetes remains controversial. This study examined the effect of HBV infection on glucose homeostasis using a duck HBV (DHBV) model. Methods: Plasma DHBV DNA was detected by quantitative polymerase chain reaction (PCR). Tissue infection of DHBV was determined by detecting DHBV covalently closed circular
-
Human herpesvirus 6 infection and risk of Chronic fatigue syndrome: a systematic review and meta-analysis Intervirology (IF 4.6) Pub Date : 2021-06-23 Sayed-Hamidreza Mozhgani, Farid Rajabi, Mohsen Qurbani, Yousef Erfani, Somayeh Yaslianifard, Azam Moosavi, Kiomars Pourrostami, Ali Baradaran Bagheri, Alireza Soleimani, Farida Behzadian, Mahshid Safavi, Farhad Rezaei
-
Evaluation of Sandwich Enzyme-Linked Immunosorbent Assay and Reverse Transcription Polymerase Chain Reaction for the Diagnosis of Foot-and-Mouth Disease Intervirology (IF 4.6) Pub Date : 2021-06-17 Salman Khan, Syed Asad Ali Shah, Syed Muhammad Jamal
Background: Foot-and-mouth disease (FMD) is an infectious and highly contagious disease of cloven-hoofed domestic and wild animals, causing heavy economic losses to the livestock industry. Rapid and reliable diagnosis of the disease is essential for the implementation of effective control measures. This study compared sandwich enzyme-linked immunosorbent assay (S-ELISA) and conventional reverse transcription
-
Gallid Alphaherpesvirus 2 in the Egyptian Turkeys: Molecular Characterization and Establishment of a Universal System for Phylogenetic Classification Intervirology (IF 4.6) Pub Date : 2021-05-21 Mahmoud Bayoumi, Mohamed El-Saied, Basem Ahmed, Magdy El-Mahdy, Haitham Amer
Introduction: Gallid alphaherpesvirus 2 (GaHV-2) is a highly contagious oncogenic virus that causes Marek’s disease in chickens and occasionally in turkeys. Among 100 genes identified in GaHV-2 genome, the Meq gene appears to involve viral virulence, oncogenicity, and genetic diversity. Despite the use of Meq gene sequences in phylogenetic classification of GaHV-2 strains circulating in many countries
-
Comparison of the Amplisure HBV Quantitative Kit with the Qiagen Artus HBV QS-RGQ Assay for Quantifying Viral DNA in Plasma Samples of Monitoring Cases Intervirology (IF 4.6) Pub Date : 2021-05-21 Ganesan Praveenkumar, Chaitali Nikam, Ragoori Venkata Ramana, Sengupta Caesar, Velumani Amruta, Ahmad Riyaj
Background: Monitoring of hepatitis B virus (HBV) viral load has become an essential phase in the treatment of HBV. There are many commercial assays available for HBV viral load quantification. In this study, we have evaluated the performance characteristics of Amplisure® HBV Kit in comparison with the Qiagen artus HBV QS-RGQ kit for HBV DNA quantitation. Methods: Comparison of 2 methods was carried
-
The Upregulation of a Novel Long Noncoding RNA AK097647 Promotes Enterovirus 71 Replication and Decreases IFN-λ1 Secretion Intervirology (IF 4.6) Pub Date : 2021-05-05 Min Chu, Bingfei Zhou, Huilin Tu, Min Li, Li Huang, Yuan He, Luo Liu, Song Han, Jun Yin, Biwen Peng, Xiaohua He, Wanhong Liu
Background: Enterovirus 71 (EV71) infects millions of children every year in China and has become a challenge to public health. However, there is no effective treatment for EV71 infection. Long noncoding RNAs (lncRNAs) have been found to play various roles in virus replication and infection. Objective: We aimed to explore the role of a novel long noncoding RNA AK097647 (lncRNA-AK097647) during EV71
-
Immune Response of Gamma-Irradiated Inactivated Bivalent Polio Vaccine Prepared plus Trehalose as a Protein Stabilizer in a Mouse Model Intervirology (IF 4.6) Pub Date : 2021-04-14 Maryam Mollaei Alamuti, Mehrdad Ravanshad, Farahnaz Motamedi-Sedeh, Arezoo Nabizadeh, Elham Ahmadi, Seyedeh Maedeh Hossieni
Introduction: Poliovirus causes paralysis by infecting the nervous system. Currently, 2 types of polio vaccine are given in many countries in polio eradication program including inactivated polio vaccine (IPV) and oral polio vaccine (OPV). Because of OPV-related paralysis, OPV should be replaced by IPV. Methods: The aim of this study was to prepare the gamma-irradiated IPV and determine its effectiveness
-
Frequency of Cytomegalovirus Viral Load in Iranian Human Immunodeficiency Virus-1-Infected Patients with CD4+ Counts <100 Cells/mm3 Intervirology (IF 4.6) Pub Date : 2021-03-31 Mohammad Reza Jabbari, Hoorieh Soleimanjahi, Somayeh Shatizadeh Malekshahi, Mohammad Gholami, Leila Sadeghi, Minoo Mohraz
Objectives: The aim of present work was to assess cytomegalovirus (CMV) viremia in Iranian human immunodeficiency virus (HIV)-1-infected patients with a CD4+ count #x3c;100 cells/mm3 and to explore whether CMV DNA loads correlate with CD4+ cell counts or associated retinitis. Methods: This study was conducted at the AIDS research center in Iran on HIV-1-infected patients with CD4+ count #x3c;100 cells/mm3
-
Bufavirus, Cosavirus, and Salivirus in Diarrheal Italian Infants Intervirology (IF 4.6) Pub Date : 2021-03-30 Valentina Daprà, Ilaria Galliano, Paola Montanari, Elena Zaniol, Cristina Calvi, Carla Alliaudi, Massimiliano Bergallo
Three newly discovered viruses have been recently described in diarrheal patients: Cosavirus (CosV) and Salivirus (SalV), 2 picornaviruses, and bufavirus (BuV), a parvovirus. The detection rate and the role of these viruses remain to be established in acute gastroenteritis (AGE) in diarrheal Italian infants. From November 2016 to November 2017, stool samples were collected from 160 children #x3c;5
-
Truncated Precursor of Feline calicivirus Major Capsid Protein: A Product Relevant for Replication, or an Aberrant Translation Artifact? Intervirology (IF 4.6) Pub Date : 2021-03-18 Ángel L. Álvarez, Francisco Parra
Intervirology
-
Infection with Hepatitis B Virus May Increase the Serum Concentrations of Osteopontin Intervirology (IF 4.6) Pub Date : 2021-03-18 Hua-Bing Liu, Qin-Yan Chen, Xue-Yan Wang, Lu-Juan Zhang, Li-Ping Hu, Tim J. Harrison, Chao Wang, Zhong-Liao Fang
Background: Serum osteopontin (OPN) concentrations were found to be significantly increased in patients infected with hepatitis B virus (HBV) and patients with hepatocellular carcinoma (HCC). Objective: The aim of this study was to determine the association among HCC, OPN, and HBV. Methods: Two hundred and forty-one subjects were recruited and divided into 6 groups: healthy controls, asymptomatic HBsAg
-
Sequence Variations of Epstein-Barr Virus-Encoded Small Noncoding RNA and Latent Membrane Protein 1 in Hematologic Tumors in Northern China Intervirology (IF 4.6) Pub Date : 2021-03-12 Hai-Yu Wang, Lingling Sun, Ping Li, Wen Liu, Zhong-Guang Zhang, Bing Luo
Objective: To investigate the relationship between hematologic tumors and Epstein-Barr virus (EBV)-encoded small noncoding RNA (EBER) variations as well as latent membrane protein 1 (LMP1) variations. Methods: Patients with leukemia and myelodysplastic syndrome (MDS) were selected as subjects. Genotypes 1/2 and genotypes F/f were analyzed using the nested PCR technology, while EBER and LMP1 subtypes
-
Molecular Phylogenetics of Hepatitis D Virus in New Zealand and the Implications for Pacific Island Countries Intervirology (IF 4.6) Pub Date : 2021-03-01 Kathy Jackson, Margaret Littlejohn, Ed Gane, Stephen Locarnini
Hepatitis delta virus (HDV) is considered a satellite virus that requires hepatitis B virus surface antigen for infectivity. HDV is endemic in some Pacific Island (PI) countries, including Kiribati and Nauru, with a unique genotype 1, “Pacific clade.” The aims of this study were to determine the HDV genotypes in New Zealand and investigate the link of strains to other PI countries and the rest of the
-
Cellular miR-101-1 Reduces Efficiently the Replication of HSV-1 in HeLa Cells Intervirology (IF 4.6) Pub Date : 2021-02-24 Bahar Sadegh Ehdaei, Ahmad Pirouzmand, Mehdi Shabani, Arezoo Mirzaei, Sharareh Moghim
Introduction: Herpes simplex viruses (HSVs) are widely distributed in the human population. HSV type 1 (HSV-1) is responsible for a spectrum of diseases, ranging from gingivostomatitis to keratoconjunctivitis, and encephalitis. The HSVs establish latent infections in nerve cells, and recurrences are common. Their frequent reactivation in elderly and immunosuppressed patients causes serious health complications
-
Viruria of Human BK Virus and John Cunningham Virus among Renal Transplant Recipients and Healthy Control in Southeast of Caspian Sea Intervirology (IF 4.6) Pub Date : 2021-02-17 Fereshteh Safaei, Alireza Mohebbi, Mina Hassanpour, Hadi Razavi Nikoo, Alijan Tabarraei
Background: Members of the Polyomaviridae family, BK virus (BKV), and John Cunningham virus (JCV) are linked to polyomavirus-associated nephropathy-associated transplant rejection in immunodeficient patients. Objective: The aim of the study was to evaluate the prevalence of BKV and JCV in immunocompetent individuals in the north of Iran. Methods: Ninety-one urine samples were obtained from renal transplant
-
Identification of Polyomaviruses in Skin Cancers Intervirology (IF 4.6) Pub Date : 2021-02-16 Pedro V.A. Costa, Patricia S. Ishiy, Paulo R.P. Urbano, Camila M. Romano, Stephen K. Tyring, Walmar R.P. Oliveira, Cyro Festa-Neto
Background: Polyomaviruses (PyVs) were initially described in animals. They have also been detected in humans with some evidence that could play a role in skin carcinogenesis. Objectives: This study aimed to verify the presence of PyVs in different skin tumour samples and to make clinical correlations with patients’ epidemiological data from Clinics Hospital of Medical School of University of São Paulo