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Low usefulness of reporting tonsillar PCR Ct-values in pharyngeal infections with Fusobacterium necrophorum Anaerobe (IF 2.3) Pub Date : 2024-02-16 David Nygren, Lisa Wasserstrom, Gustav Torisson, Karin Holm
Tonsillar PCR Ct-values were higher in participants with asymptomatic tonsillar carriage than patients with pharyngeal infections. However, Ct-values were not associated with severity of disease or predictive of development of complications and hence lacked clinical usefulness. The reporting of Ct-values in clinical samples is not recommended.
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Novel and rare β-lactamase genes of Bacteroides fragilis group species: Detection of the genes and characterization of their genetic backgrounds Anaerobe (IF 2.3) Pub Date : 2024-02-13 Bakhtiyar Mahmood, Károly Péter Sárvári, Laszló Orosz, Elisabeth Nagy, József Sóki
This study screened the prevalence of rare β-lactamase genes in group strains from clinical specimens and normal microbiota and examined the genetic properties of the strains carrying these genes. blaHGD1, OXA347, , , and were detected by real-time polymerase chain reaction in collections of strains from clinical (n = 406) and fecal (n = 184) samples. To examine the genetic backgrounds of the samples
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Microscopy methods for Clostridioides difficile Anaerobe (IF 2.3) Pub Date : 2024-02-08 Chenlin Hu, Kevin W. Garey
Microscopic technologies including light and fluorescent, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and cryo-electron microscopy have been widely utilized to visualize C at the molecular, cellular, community, and structural biology level. This comprehensive review summarizes the microscopy tools (fluorescent and reporter system) in their use to study different aspects
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The burden of Clostridioides difficile infections in South-East Asia and the Western Pacific: A narrative review Anaerobe (IF 2.3) Pub Date : 2024-02-07 Frederick J. Angulo, Canna Ghia, Mark A. Fletcher, Egemen Ozbilgili, Graciela Morales
(formerly ) is well-documented in Europe and North America to be a common cause of healthcare-associated gastrointestinal tract infections. In contrast, infection (CDI) is infrequently reported in literature from Asia, which may reflect a lack of clinician awareness. We conducted a narrative review to better understand CDI burden in Asia. We searched the PubMed database for English language articles
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Polyphasic molecular approach to the characterization of methanogens in the saliva of Tunisian adults Anaerobe (IF 2.3) Pub Date : 2024-02-02 Fériel Bouzid, Imen Gtif, Salma Charfeddine, Leila Abid, Najla Kharrat
Methanogenic archaea are a minor component of human oral microbiota. Due to their relatively low abundance, the detection of these neglected microorganisms is challenging. This study concerns the presence of methanogens in salivary samples collected from Tunisian adults to evaluate their prevalence and burden using a polyphasic molecular approach. A total of 43 saliva samples were included. Metagenomic
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Bacteroides and related species: The keystone taxa of the human gut microbiota Anaerobe (IF 2.3) Pub Date : 2024-01-10 Jae Hyun Shin, Glenn Tillotson, Tiffany N. MacKenzie, Cirle A. Warren, Hannah M. Wexler, Ellie J.C. Goldstein
Microbial communities play a significant role in maintaining ecosystems in a healthy homeostasis. Presently, in the human gastrointestinal tract, there are certain taxonomic groups of importance, though there is no single species that plays a keystone role. Bacteroides spp. are known to be major players in the maintenance of eubiosis in the human gastrointestinal tract. Here we review the critical
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Clinical and microbiological characteristics of Ruminococcus gnavus bacteremia and intra-abdominal infection Anaerobe (IF 2.3) Pub Date : 2024-01-10 Naoki Watanabe, Tomohisa Watari, Yoshihito Otsuka, Naoto Hosokawa, Kazufumi Yamagata, Miyuki Fujioka
Objectives Ruminococcus gnavus is a rare human pathogen, and clinical data on R. gnavus infection are insufficient. This retrospective study aimed to investigate the clinical characteristics of R. gnavus infections. Methods This study included 13 cases of bacteremia and three cases of non-bacteremia infections caused by R. gnavus. We evaluated the patient data, infection source, clinical outcomes,
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Production and purification of Clostridium perfringens type C beta-toxin and IgG and IgY antitoxins Anaerobe (IF 2.3) Pub Date : 2023-12-30 Guilherme Guerra Alves, Ronnie Antunes Assis, Victor Santos do Amarante, Carlos Augusto de Oliveira Júnior, Rodrigo Otávio Silveira Silva, Luiz Guilherme Dias Heneine, Francisco Carlos Faria Lobato
Objectives This study aimed to produce and purify Clostridium perfringens type C beta-toxin, sheep anti-beta toxin immunoglobulin G (IgG) and chicken immunoglobulin Y (IgY). Methods Two methods were used for beta-toxin purification: single-step metal affinity chromatography (MAC) using zinc as a chelator and ion exchange chromatography (IEX). The purified and inactivated beta-toxoids were then administered
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In vitro activity of delafloxacin against anaerobic bacteria compared with other antimicrobials Anaerobe (IF 2.3) Pub Date : 2023-12-23 Fernando Cobo, Ana Franco-Acosta, Lina Martín-Hita, Javier Rodríguez-Granger, Antonio Sampedro-Martínez, Juan Antonio Reguera-Márquez, José María Navarro-Marí
The aim of this study was to describe the in vitro activity of delafloxacin against 230 anaerobic isolates and compare it with the activity of other antimicrobials used against infections caused by anaerobic microorganisms. Minimal inhibitory concentrations (MICs) were lower for delafloxacin than for all other antibiotics tested with the exception of piperacillin-tazobactam and meropenem against Gram-positive
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Enhancing biogas generation from lignocellulosic biomass through biological pretreatment: Exploring the role of ruminant microbes and anaerobic fungi Anaerobe (IF 2.3) Pub Date : 2023-12-23 R. Tamilselvan, A. Immanuel Selwynraj
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TMT-based quantitative proteomics reveals the nutritional and stress resistance functions of anaerobic fungi in yak rumen during passage at different time intervals Anaerobe (IF 2.3) Pub Date : 2023-12-02 Jingrong Zhang, Yaqin Wei, Huizhen Qiu, Jiayi Han
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Evaluation of the additive effects of volatile fatty acids and moderate heat treatment for enhancing the inactivation of vegetative cells and spores of Clostridium perfringens by flow cytometry Anaerobe (IF 2.3) Pub Date : 2023-11-23 Cedric Delmon, Catherine Ouk, Magali Casellas, Audrey Prorot
Objectives Clostridium perfringens is a well-known spore-forming bacterium that can resist the environment. A mixture of volatile fatty acids or thermal treatments can interact with these bacteria. The aim of this study was to evaluate the effects of different volatile fatty acid concentrations and moderate heat treatment on Clostridium perfringens sporulation. Methods A pure culture of Clostridium
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Brain abscess caused by Porphyromonas gingivalis and Eubacterium nodatum mimicking acute stroke Anaerobe (IF 2.3) Pub Date : 2023-11-23 Domingo Fernández Vecilla, Mary Paz Roche Matheus, Mikel Urrutikoetxea Gutiérrez, Gotzon Iglesias Hidalgo, Cristina Aspichueta Vivanco, José Luis Díaz de Tuesta del Arco
We described a case of a 65-year-old man with a brain abscess caused by Porphyromonas gingivalis and Eubacterium nodatum. The patient presented right central facial nerve palsy, mutism and right hemiparesis at the examination. The patient underwent a left frontal craniotomy with evacuation of the brain abscess. Specimens were collected for microbiological analysis and intravenous treatment was started
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Clinical usefulness of anaerobic blood culture in pediatric patients with bacteremia Anaerobe (IF 2.3) Pub Date : 2023-11-20 Ga Young Noh, Yoon Soo Park, Si Hyun Kim, Sae Am Song, Jeong Hwan Shin
Objectives The purpose of this study is to evaluate the usefulness of anaerobic blood culture in pediatric patients by comparing the detection rate and distribution of bacteria between aerobic and anaerobic blood culture bottles. Methods A retrospective analysis was conducted on 11,664 blood cultures obtained from children under the age of 14 between January 2013 and June 2020. The positive rate of
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Antimicrobial susceptibility testing of anaerobic bacteria causing bacteremia: A 13-year (2010–2022) retrospective study in a tertiary hospital Anaerobe (IF 2.3) Pub Date : 2023-11-19 Jorge Ligero-López, Eduardo Rubio-Mora, Mario David Ruiz-Bastián, María Inmaculada Quiles-Melero, Juana Cacho-Calvo, Emilio Cendejas-Bueno
Infections from anaerobic microorganisms result from breached mucosal barriers, posing a significant mortality risk. A retrospective study at Hospital Universitario La Paz (Madrid) from 2010 to 2022 analyzed 491 (6.17 %) anaerobic bacteremia cases out of 7956 significant bacteremia cases among 171,833 blood culture requests. Bacteroides fragilis was the most frequently isolated species (28.3 %), followed
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Efficacy of bezlotoxumab to prevent recurrent Clostridioides difficile infection (CDI) in patients with multiple prior recurrent CDI Anaerobe (IF 2.3) Pub Date : 2023-11-04 Andrew M. Skinner, Xing Tan, Colin Westman, Thomas Birris, Emily N. Drwiega, Michael S. Wang, Stuart Johnson
Among 23 patients with multiply recurrent Clostridiodies difficile infection (mrCDI) who received bezlotoxumab at the end of antibiotic treatment a sustained clinical response of 91 % at 30 days and 78 % at 90 days was achieved. Bezlotoxumab administered at the end of antibiotic treatment was effective in patients with mrCDI.
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Description of Fusibacillus kribbianus gen. nov., sp. nov., a fusiform anaerobe isolated from pig feces Anaerobe (IF 2.3) Pub Date : 2023-11-02 Lu Bai, Jayoung Paek, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang
Objective The family Lachnospiraceae is affiliated with the order Clostridiales and was originally contained within Clostridial cluster XIVa. The members of Lachnospiraceae inhabiting the gut comprise the chemoorganotrophic genera, generating sundry short-chain fatty acids to supply energy to the host, and are considered to be related to obesity and gut health. Methods The polyphasic taxonomic approach
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Identification of root canal microbiota profiles of periapical tissue diseases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer Anaerobe (IF 2.3) Pub Date : 2023-11-02 Mete Ahlat, Cumhur Aydin, Sinem Kaya, Mehmet Baysallar
Objectives The purpose of this study was to identify microorganisms isolated from various periapical tissue diseases using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and classify them via an unsupervised machine learning approach. Methods A total of 150 patients with various apical conditions and teeth in need of endodontic retreatment were divided into
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Growth assessment of mixed cultures of probiotics and common pathogens Anaerobe (IF 2.3) Pub Date : 2023-10-30 Mansa Fredua-Agyeman, Paul Stapleton, Simon Gaisford
Objectives In this work, an isothermal microcalorimeter was applied to investigate the antipathogenic activity of three probiotics (Lactobacillus acidophilus, Bifidobacterium lactis and Bifidobacterium bifidum) against Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli using the probiotics in mixed culture with the pathogenic microorganisms. Methods A microcalorimeter was used to monitor
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Oral teicoplanin administration suppresses recurrence of Clostridioides difficile infection: Proof of concept Anaerobe (IF 2.3) Pub Date : 2023-10-23 Yoko Tanaka, Sho Tashiro, Shintaro Ikegami, Yuki Enoki, Kazuaki Taguchi, Kazuaki Matsumoto
Objectives Teicoplanin is a potential antimicrobial candidate for Clostridioides difficile infection (CDI) treatment. However, the therapeutic potential of teicoplanin against severe CDI has not been clinically proven. In the present study, we investigated the efficacy of oral teicoplanin administration against severe CDI and the recurrence of severe CDI after teicoplanin treatment in a mouse model
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Impact of nicotine and cotinine on macrophage inflammatory plasticity via vesicular modifications in gastrointestinal bacteria Anaerobe (IF 2.3) Pub Date : 2023-10-10 Jourdan E. Lakes, Xu Fu, Brock T Harvey, Khaga R. Neupane, Surya P. Aryal, Jessica L. Ferrell, Michael D. Flythe, Christopher I. Richards
Objectives This study aimed to elucidate mechanistic explanation(s) for compositional changes to enteric microflora by determining the impacts of continuous nicotine/cotinine exposure on representative gastrointestinal bacteria and how these alterations impact innate immune cell plasticity. Methods In vitro cultures of the gastrointestinal bacteria (Bacteroides fragilis 25285, Prevotella bryantii B14
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Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer Anaerobe (IF 2.3) Pub Date : 2023-10-06 Yuriko Matsumiya, Mitsukuni Suenaga, Toshiaki Ishikawa, Toshifumi Kudo, Tsuyoshi Nakagawa, Kentaro Okamoto, Masanori Tokunaga, Claudia Hurtado, Yuki Yamada, Kentaro Oka, Motomichi Takahashi, Luis Francisco Lopez Kostner, Miguel Luis O'Ryan Gallardo, Hiroyuki Uetake, Yusuke Kinugasa
Introduction Bacteroides fragilis (B. fragilis) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic B. fragilis (ETBF), a subtype of B. fragilis, produces an enterotoxin (BFT; B. fragilis toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of B. fragilis and ETBF on the clinical outcome of colorectal
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The impact of Akkermansia muciniphila and its extracellular vesicles in the regulation of serotonergic gene expression in a small intestine of mice Anaerobe (IF 2.3) Pub Date : 2023-10-04 Rezvan Yaghoubfar, Ehsan Zare BanadKoki, Fatemeh Ashrafian, Arefeh Shahryari, Arian Kariman, Mehdi Davari, Abolfazl Fateh, Shohreh Khatami, Seyed Davar Siadat
Objectives A better understanding of host-microbe interactions as a cross-talk between the gastrointestinal (GI) tract and the gut microbiota can help treat and prevent GI disorders by improving the maintenance of GI homeostasis. The gut microbiota can affect signaling molecules, such as serotonin, which regulates endocrine systems through the GI tract. Moreover, studying the effects of gut microbiota
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Metabolism of azo food dyes by bacterial members of the human gut microbiome Anaerobe (IF 2.3) Pub Date : 2023-09-27 Riley Elder, Sarah J. Vancuren, Alexander J. Botschner, P. David Josephy, Emma Allen-Vercoe
Objectives We set out to survey the capacities of bacterial isolates from the human gut microbiome to reduce common azo food dyes in vitro. Methods A total of 206 strains representative of 124 bacterial species and 6 phyla were screened in vitro using a simple azo dye decolorization assay. Strains which showed azoreductive activity were characterized by studies of azoreduction kinetics and bacterial
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Upregulation of the cfiA carbapenemase gene in a Bacteroides fragilis strain by the novel integrative and conjugative element Tn7563 Anaerobe (IF 2.3) Pub Date : 2023-09-23 Tore Taksdal Stubhaug, Nermin Zecic, Dagfinn Skaare
We describe Tn7563, a 31,844-bp integrative and conjugative element (ICE) carrying promoters upregulating the cfiA carbapenemase gene in Bacteroides fragilis strain Tbg-22. Excision and circularization of Tn7563 was demonstrated by PCR. Previously, only insertion sequences (IS) have been shown to carry mobile promoters for cfiA.
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Granular activated carbon enhances the anaerobic digestion of solid and liquid fractions of swine effluent at different mesophilic temperatures Anaerobe (IF 2.3) Pub Date : 2023-09-17 Miriam Orrantia, Edna R. Meza-Escalante, Vianey A. Burboa-Charis, Refugio B. García-Reyes, Marina M. Atilano-Camino, Denisse Serrano-Palacios, Luis A. Leyva, Yair A. Del Angel, Luis H. Alvarez
Objectives This study evaluated the effect of particle size and dosage of granular activated carbon (GAC) on methane production from the anaerobic digestion of raw effluent (RE) of swine wastewater, and the solid (SF) and liquid (LF) fractions. The effect of temperature using the selected size and dosage of GAC was also evaluated. Methods 60 mL of swine wastewater were inoculated with anaerobic granular
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Effect of formalin percentage, incubation time and temperature on Clostridium chauvoei culture inactivation and immunogenicity Anaerobe (IF 2.3) Pub Date : 2023-09-01 Niusha Adib, Azadeh Zahmatkesh, Parvaneh Esmaeilnejad-Ahranjani, Alireza Paradise, Lida Abdolmohammadi Khiav, Masoumeh Bagheri, Mohammad Abdoli, Atoosa Adib
Objectives In order to find the optimal inactivation conditions for Clostridium chauvoei culture, different factors were investigated and the immunogenicity of inactivated cultures was studied. Methods C. chauvoei was cultured with different formalin percentages (0.3, 0.5 or 0.7% V/V), inactivation temperatures (37 °C or room temperature) and incubation times (one or two weeks). Sterility tests were
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Pseudodesulfovibrio thermohalotolerans sp. nov., a novel obligately anaerobic, halotolerant, thermotolerant, and sulfate-reducing bacterium isolated from a western offshore hydrocarbon reservoir in India Anaerobe (IF 2.3) Pub Date : 2023-08-22 Saurabh L. Gaikwad, Soham D. Pore, Prashant K. Dhakephalkar, Sumit Singh Dagar, Rajeev Soni, Maninder Pal Kaur, Harshita Negi Rawat
Objective Characterization and documentation of strain MCM B-1480T, a novel sulfate-reducing bacterium isolated from produced water of India's western offshore hydrocarbon reservoir. Method Strain MCM B-1480T was unequivocally identified using a polyphasic approach routinely followed in bacterial systematics. The morphological and biochemical characterization of strain MCM B-1480T was carried out using
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Common laboratory tests and their correlation with the clinical presentation and prognosis of lemierre syndrome Anaerobe (IF 2.3) Pub Date : 2023-08-16 Riccardo M. Fumagalli, Elvira Gloor, Philippe A. Kaufmann, Maurus Frehner, Davide Voci, Stavros V. Konstantinides, Nils Kucher, Tommaso F. Nicoletti, Alessandro Pecci, Luca Valerio, Stefano Barco
Introduction Lemierre syndrome is a thromboembolic complication following an acute bacterial infection of the head/neck area, often due to anaerobes. Data on the prognostic role of laboratory parameters is lacking. Methods We analyzed individual-patient level data from a multinational cohort of patients with Lemierre-syndrome. Patients had an infection in the head/neck area, and contiguous vein thrombosis
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Prevalence of Clostridioides difficile in dogs (Canis familiaris) with gastrointestinal disorders in Rio de Janeiro Anaerobe (IF 2.3) Pub Date : 2023-08-12 Suzana Leite, Carlos Cotias, Kelly C. Rainha, Mayara Gil Santos, Bruno Penna, Renata F. F.Moraes, Céline Harmanus, Wiep Klaas Smits, Eliane de Oliveira Ferreira
Clostridioides difficile infections (CDI) have a high morbidity and mortality rate and have always been considered a nosocomial disease. Nonetheless, the number of cases of community-acquired CDI is increasing, and new evidence suggests additional C. difficile reservoirs exist. Pathogenic C. difficile strains have been found in livestock, domestic animals, and meat, so a zoonotic transmission has been
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Bacteremia due to Clostridium innocuum: Analysis of four cases and literature review Anaerobe (IF 2.3) Pub Date : 2023-08-09 Fernando Cobo, Virginia Pérez-Carrasco, María Tarriño-León, María Aguilera-Franco, José A. García-Salcedo, José María Navarro-Marí
Clostridium innocuum is a Gram-positive anaerobic spore-forming bacillus that has been identified as part of the normal intestinal microbiota. This bacterium has been rarely associated with human infections, and only few severe infections have been reported until now. In this work, we report on four patients with bacteremia due to C. innocuum, which were well identified by MALDI-TOF MS. Moreover, a
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The role of microbiome-based therapeutics for the reduction and prevention of antimicrobial-resistant organism colonization Anaerobe (IF 2.3) Pub Date : 2023-08-10 Armaghan-e-Rehman Mansoor, Caroline A. O'Neil, Jennie H. Kwon
The gut is host to a diverse array of microbiota that constitute a complex ecological system crucial to human physiology. Disruptors to the normal host microbiota, such as antimicrobials, can cause a loss of species diversity in the gut, reducing its ability to resist colonization by invading pathogens and potentially leading to colonization with antimicrobial resistant organisms (AROs). ARO negatively
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Probiotic-related Clostridium butyricum bacteremia: A case report and literature review Anaerobe (IF 2.3) Pub Date : 2023-08-05 Kazuhiro Ishikawa, Ryo Hasegawa, Koko Shibutani, Yumiko Mikami, Fujimi Kawai, Takahiro Matsuo, Yuki Uehara, Nobuyoshi Mori
We report three cases of Clostridium butyricum bacteremia associated with taking C. butyricum-related probiotics. We performed a literature review and found 11 cases of C. butyricum bacteremia including our cases. Nine cases related to probiotics. We should consider that probiotics may infect clinically unstable patients.
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Autolysin as a fibronectin receptor on the cell surface of Clostridium perfringens Anaerobe (IF 2.3) Pub Date : 2023-08-06 Riyo Aono, Shogo Emi, Kanako Okabe-Watanabe, Hirofumi Nariya, Nozomu Matsunaga, Yasuo Hitsumoto, Seiichi Katayama
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The outer membrane protein of Fusobacterium necrophorum, 43K OMP, stimulates inflammatory cytokine production through nuclear factor kappa B activation Anaerobe (IF 2.3) Pub Date : 2023-08-02 Xianjing He, Jiao Liu, Kai Jiang, Shuai Lian, Yu Shi, Shan Fu, Pengyu Zhao, Jiawei Xiao, Dongbo Sun, Donghua Guo
Objective Fusobacterium necrophorum causes bovine hepatic abscess, foot rot, mastitis, and endometritis. The 43 kDa outer membrane protein (43 K OMP) of F. necrophorum is a porin protein that plays an important role in infections by this bacterium, but the biological function and the pathogenesis of this protein are largely unknown. Methods In this study, we investigated the role of the 43 K OMP in
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The challenges of diagnosis and treatment of the rare Prevotella induced breast abscess: A retrospective cohort study Anaerobe (IF 2.3) Pub Date : 2023-07-25 I.M. Brouwer de Koning, S.W.T. Hoogmoet, N.H.M. Renders, Y.C.G.J. Paquay, M. Bessems, W.A. Draaisma, K. Bosscha
Objective Although the genus Prevotella is part of the general human flora, species of this anaerobic gram-negative bacteria have been described as cause of persisting nonpuerperal breast abscesses. Collecting punctate samples and testing these samples for anaerobic bacteria, is not part of the common diagnostic workflow in atypical breast abscesses. The causative anaerobic micro-organism can remain
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Ruminococcus gnavus bacteremia: Literature review and a case report associated to acute flare of ulcerative colitis in an immunocompromised patient Anaerobe (IF 2.3) Pub Date : 2023-07-21 Javier Martínez de Victoria Carazo, David Vinuesa García, Esther Serrano-Conde Sánchez, José Antonio Peregrina Rivas, Antonio José Ruíz Rodríguez, José Hernández Quero
We present a case of bacteremia caused by Ruminococcus gnavus in an immunocompromised patient. R. gnavus is a Gram-positive strict anaerobe bacterium that forms chains. The bacteremia has been associated with an acute flare of ulcerative colitis. Anaerobic bacteremia is becoming increasingly frequent in patients with compromised gastrointestinal barrier. The role of the human microbiota and its alterations
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The role of Actinomyces spp. And related organisms in cervicofacial infections: Pathomechanism, diagnosis and therapeutic aspects Anaerobe (IF 2.3) Pub Date : 2023-07-22 Zsanett Kövér, Vidar Johansen Nordskag, Ágnes Bán, Márió Gajdács, Edit Urbán
Members of the Actinomyces genus and Actinomyces-like organisms (ALOs; namely Actinotignum, Arcanobacterium, Schaalia and Varibaculum) are Gram-positive, non-spore-forming rods that are commensal members of the human oral cavity, gastrointestinal tract, female genital tract and skin flora. Cervicofacial actinomycosis or “lumpy jaw syndrome” – the chronic, suppurative granulomatous disease caused by
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Survival of bovine digital dermatitis treponemes in conditions relevant to the host and farm environment Anaerobe (IF 2.3) Pub Date : 2023-07-20 Jennifer Bell, Hayley E. Crosby-Durrani, Roger W. Blowey, Stuart D. Carter, Nicholas J. Evans
Objectives Bovine digital dermatitis (BDD), a painful infectious foot disease in dairy cattle, endemic in many countries worldwide, causes substantial economic and welfare impacts. Treponema spp. are considered key to BDD pathogenesis. To aid infection reservoir identification and control measure development, survival of BDD treponemes was investigated in different temperatures (4, 12, 20, 37, 45 and
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Biology activity and characterization of the functional L-HN fragment derivative of botulinum neurotoxin serotype E Anaerobe (IF 2.3) Pub Date : 2023-07-20 Xiao Tan, Cong-Cong Zhang, Jian-Sheng Lu, Zhi-Ying Li, Bo-Lin Li, Xu-Yang Liu, Yun-Zhou Yu, Qing Xu
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Membrane vesicles of Lactobacillus gasseri ATCC 19992 disrupt biofilms of vaginal pathogens Anaerobe (IF 2.3) Pub Date : 2023-07-17 Sameen Khan, Parul Shishpal, Vikrant M. Bhor
Membrane vesicles (MVs) are bioactive, nano-sized entities produced by all organisms. MVs of L. gasseri ATCC 19992 were isolated and their effect on the biofilms of vaginal pathogens, G. vaginalis and S. aureus was studied. The L. gasseri MVs resulted in significant disruption of biofilms of the vaginal pathogens.
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Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong, 2020–2021 Anaerobe (IF 2.3) Pub Date : 2023-07-08 Hanshu Fang, Xin Li, Mei-Kum Yan, Man-Ki Tong, Kin-Hung Chow, Vincent Chi-Chung Cheng, Pak-Leung Ho
Objectives This retrospective study analyzed the susceptibility levels of Bacteroides fragilis group (BFG) in a hospital-based laboratory where disk diffusion test (DDT) was routinely performed. Isolates non-susceptible to imipenem and metronidazole by DDT were further investigated by gradient method. Methods The DDT and MIC susceptibility data of clindamycin, metronidazole, moxifloxacin and imipenem
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Type IV pili facilitated natural competence in Fusobacterium nucleatum Anaerobe (IF 2.3) Pub Date : 2023-07-13 Blake E. Sanders, Ariana Umaña, Tam T.D. Nguyen, Kevin J. Williams, Christopher C. Yoo, Michael A. Casasanta, Bryce Wozniak, Daniel J. Slade
Objectives Many bacterial species naturally take up DNA from their surroundings and recombine it into their chromosome through homologous gene transfer (HGT) to aid in survival and gain advantageous functions. Herein we present the first characterization of Type IV pili facilitated natural competence in Fusobacterium nucleatum, which is a Gram-negative, anaerobic bacterium that participates in a range
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Strain specificity in fusobacterial co-aggregation with colorectal cancer-relevant species Anaerobe (IF 2.3) Pub Date : 2023-07-08 Avery V. Robinson, Emma Allen-Vercoe
Objectives The purpose of the present study was to characterize co-aggregation interactions between isolates of Fusobacterium nucleatum subsp. animalis and other colorectal cancer (CRC)-relevant species. Methods Co-aggregation interactions were assessed by comparing optical density values following 2-h stationary strain co-incubations to strain optical density values when incubated alone. Co-aggregation
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Description of eight human infections caused by Bacteroides pyogenes in a tertiary hospital of northern Spain Anaerobe (IF 2.3) Pub Date : 2023-07-05 Domingo Fernández Vecilla, Mikel Joseba Urrutikoetxea Gutiérrez, Mary Paz Roche Matheus, Itziar Angulo López, Cristina Aspichueta Vivanco, Felicitas Elena Calvo Muro, José Luis Díaz de Tuesta del Arco
Bacteroides pyogenes is a Gram-negative obligate anaerobe, saccharolytic, non-motile, non-pigment-producing and non-spore-forming rod. Reports of human infections caused by B. pyogenes are scarce, with approximately 30 cases documented in scientific literature. The aim of this study was to describe the clinical characteristics of 8 different patients and to study the in vitro antibiotic susceptibility
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Clostridioides difficile PCR Tcdb Cycle Threshold predicts toxin EIA positivity but not severity of infection Anaerobe (IF 2.3) Pub Date : 2023-07-03 Regan Mah, Kerstin Locher, Theodore S. Steiner, Aleksandra Stefanovic
Background Diagnosis of Clostridioides difficile Infection (CDI) entails compatible clinical presentation and laboratory findings. We evaluated real-time polymerase chain reaction (qPCR) cycle threshold (CT) as a predictor for disease severity and TcdB enzyme immunoassay (EIA) results. Methods Inpatients or emergency department patients who tested positive for tcdB gene by PCR were evaluated. Patients’
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Comparison of microbiological profile of enterotoxigenic Bacteroides fragilis (ETBF) isolates from subjects with colorectal cancer (CRC) or intestinal pre-cancerous lesions versus healthy individuals and evaluation of environmental factors involved in intestinal dysbiosis Anaerobe (IF 2.3) Pub Date : 2023-06-26 Patrizia Spigaglia, Fabrizio Barbanti, Elena Angela Pia Germinario, Enrico Maria Criscuolo, Giovanni Bruno, Lupe Sanchez-Mete, Barbara Porowska, Vittoria Stigliano, Fabio Accarpio, Andrea Oddi, Ilaria Zingale, Silvia Rossi, Roberta De Angelis, Alessia Fabbri
Objective The aim of this study was to analyze enterotoxigenic Bacteroides fragilis (ETBF) isolates from colorectal biopsies of subjects with a histological analysis positive for colorectal cancer (CRC), pre-cancerous lesions (pre-CRC) or with a healthy intestinal tissue and to evaluate the environmental factors that may not only concur to CRC development but may also affect gut microbiota composition
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Comparative evaluation of MALDI-TOF MS and 16S rRNA gene sequencing for the identification of clinically relevant anaerobic bacteria. Critical evaluation of discrepant results Anaerobe (IF 2.3) Pub Date : 2023-06-14 Fernando Cobo, Virginia Pérez-Carrasco, Lina Martín-Hita, José Antonio García-Salcedo, José María Navarro-Marí
Objectives The main study objective was to evaluate the correlation between matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing results for the identification of anaerobes. Methods A retrospective study was conducted of all anaerobic bacteria isolated from clinically significant specimens. MALDI-TOF (Bruker Byotyper) and 16S rRNA
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Spore-forming properties and enhanced oxygen tolerance of butyrate-producing Anaerostipes spp. Anaerobe (IF 2.3) Pub Date : 2023-06-08 Ren Kadowaki, Hiroki Tanno, Shintaro Maeno, Akihito Endo
Objectives Butyrate producing bacteria are promising candidates for next-generation probiotics. However, they are extremely sensitive to oxygen, which is a significant obstacle to their inclusion in food matrices in a viable form. The present study characterized the spore-forming properties and stress tolerance of human gut butyrate-producing Anaerostipes spp. Methods Spore formation properties in
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Prosthetic joint infection caused by an atypical gram-negative bacilli: Odoribacter splanchnicus Anaerobe (IF 2.3) Pub Date : 2023-06-12 Yuri Lara-Taranchenko, Pablo S. Corona, Dolors Rodríguez-Pardo, Paula Salmerón-Menéndez, Marina Vicente Ciurans, María Cristina García-Martínez, Lluís Carrera Calderer
Prosthetic joint infection (PJI) is a devastating complication after total hip arthroplasty. Its management consists of both: a radical debridement and implant retention or exchange (depending on the timing of symptoms) and directed antibiotic therapy. Thus, the isolation of atypical microorganisms implies a challenge, where anaerobes are responsible for only 4% of cases. However, Odoribacter splanchnicus
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Clostridium perfringens epsilon-toxin requires acid sphingomyelinase for cellular entry Anaerobe (IF 2.3) Pub Date : 2023-06-10 Yoshihiko Sakaguchi, Keiko Kobayashi, Masaya Takehara, Masahiro Nagahama
Objectives Clostridium perfringens epsilon-toxin is considered to be a crucial agent in enterotoxemia in domestic animals. Epsilon-toxin enters host cells via endocytosis and results in the formation of late endosome/lysosome-derived vacuoles. In the present study, we found that acid sphingomyelinase promotes the internalization of epsilon-toxin in MDCK cells. Methods We measured the extracellular
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Community-associated Clostridioides difficile infection in a general hospital from Argentina Anaerobe (IF 2.3) Pub Date : 2023-06-09 Barbara Fox, Valentina Ricci, Silvina Bergese, Pablo Striebeck, Ana Schneider, María Alejandra Berger, María Ivana Maldonado, Liliana Fernandez-Canigia
Toxin-producing Clostridioides difficile infection (CDI) is the leading cause of hospital-acquired diarrhea. However, it is now recognized as a cause of diarrhea in the community. This single-center study aimed to determine the epidemiological origin of CDI cases between January 2014 and December 2019 and to compare demographic characteristics, comorbidities, risk factors, severity, and mortality of
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Anaerobes in specific infectious and noninfectious diseases: new developments. Anaerobe (IF 2.3) Pub Date : 2023-06-01 Lyudmila Boyanova,Rumyana Markovska,Daniel Yordanov,Raina Gergova,Petyo Hadzhiyski
With the buildup of new research data, newer associations between anaerobic bacteria and diseases/conditions were evaluated. The aim of the mini-review was to draw attention and to encourage further multidisciplinary studies of the associations. We considered microbiome-disease correlations such as a decrease of fecal Faecalibacterium prausnitzii abundance in inflammatory bowel disease (IBD) and IBD
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Antimicrobial susceptibility testing of Bacteroides species by disk diffusion: The NordicAST Bacteroides study Anaerobe (IF 2.3) Pub Date : 2023-05-28 Tore Taksdal Stubhaug, Christian G. Giske, Ulrik S. Justesen, Gunnar Kahlmeter, Erika Matuschek, Arnfinn Sundsfjord, Dagfinn Skaare
Objectives – Antimicrobial susceptibility testing (AST) of anaerobic bacteria has until recently been done by MIC methods. We have carried out a multi-centre evaluation of the newly validated EUCAST disk diffusion method for AST of Bacteroides spp. Methods – A panel of 30 Bacteroides strains was assembled based on reference agar dilution MICs, resistance gene detection and quantification of cfiA carbapenemase
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Expanding the repertoire of counterselection markers for markerless gene deletion in the human gut bacterium Phocaeicola vulgatus Anaerobe (IF 2.3) Pub Date : 2023-05-25 André Neff, Rebecca Lück, Marcel Hövels, Uwe Deppenmeier
Objective Phocaeicola (P.) vulgatus (formerly Bacteroides vulgatus) is a highly abundant and ubiquitous member of the human gut microbiota, associated with human health and disease, and therefore represents an important target for further investigations. In this study a novel gene deletion method was developed for P. vulgatus, expanding the tools available for genetic manipulation of members of the
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Clostridioides (Clostridium) difficile in adults with diarrhoea in Vietnam Anaerobe (IF 2.3) Pub Date : 2023-05-25 Peng An Khun, Long Duc Phi, Huong Thi Thu Bui, Deirdre A. Collins, Thomas V. Riley
Background Clostridioides (Clostridium) difficile causes antimicrobial-associated diarrhoea, however, presentations may range from asymptomatic carriage to severe diarrhoea, life-threatening toxic megacolon and even death. Reports on C. difficile infection (CDI) in Vietnam remain limited. The objectives of this study were to evaluate the epidemiology, molecular characteristics, and antimicrobial susceptibility
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The effects of identical nim gene-insertion sequence combinations on the expression of the nim genes and metronidazole resistance in Bacteroides fragilis strains Anaerobe (IF 2.3) Pub Date : 2023-05-19 Bakhtiyar Mahmood, Hajnalka Juhász, David Leitsch, József Sóki
In this study we examined whether the same nim gene-insertion sequence (IS) element combinations give rise to the same expression levels as they harbor shared IS element-borne promoters. From our quantitative analysis, we found that the expressions of the nimB and nimE genes with their cognate IS elements were similar, but the metronidazole resistance of these strains were more diverse.
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Risk factors for recurrent healthcare-facility associated Clostridioides difficile infection in a Swedish setting Anaerobe (IF 2.3) Pub Date : 2023-05-20 Johan Karp, Jon Edman-Wallér, Michael Toepfer, Gunnar Jacobsson
Purpose The objectives were to determine the risk factors for recurrent healthcare facility-associated Clostridioides difficile infection (HCF-CDI) in a high CDI incidence, low antibiotic use setting and to determine if length of cefotaxime exposure is a risk factor for recurrent HCF-CDI. Methods The risk factors for recurrent HCF-CDI were evaluated with a retrospective nested case control study based
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In-vitro determination of inhibitory effects of probiotic strains on Clostridioides difficile Anaerobe (IF 2.3) Pub Date : 2023-05-20 Gizem Taylan Yalçin, Melike Nur Tosun, Nükhet Nilüfer Demirel Zorba