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Phospholipase D, a Novel Therapeutic Target Contributes to the Pathogenesis of Neurodegenerative and Neuroimmune Diseases Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-3-7 Weiwei Zhang, Feiqi Zhu, Jie Zhu, Kangding Liu
Phospholipase D (PLD) is an enzyme that consists of six isoforms (PLD1–PLD6) and has been discovered in different organisms including bacteria, viruses, plants, and mammals. PLD is involved in regulating a wide range of nerve cells’ physiological processes, such as cytoskeleton modulation, proliferation/growth, vesicle trafficking, morphogenesis, and development. Simultaneously, PLD, which also plays
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miR-224-5p Attenuates Allergic Responses in Mice with Allergic Rhinitis by Modulating the Th1/Th2 Response Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-2-29 Yuxiao Li, Ran An, Mingjin Wu, Jiayan He, Xiaoguang He
Background. Allergic rhinitis (AR) is a common chronic respiratory disease that has become a global health problem. miRNAs play an important role in multiple immune and inflammatory diseases, including AR. In this work, the mechanism by which miR-224-5p regulates AR in vivo and in vitro was examined. Methods. Human nasal epithelial cells (HNEpCs) were used to establish an AR cell model induced by Der
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Hsa_circ_0000190 Promotes NSCLC Cell Resistance to Cisplatin via the Modulation of the miR-1253/IL-6 Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-2-26 Hua He, Tian Li
Background. This study explored the mechanistic basis for nonsmall cell lung cancer (NSCLC) cisplatin (DDP) treatment resistance in an effort to define effective approaches to abrogating the emergence of such chemoresistance. Methods. Analyses of NSCLC expression of hsa_circ_0000190, miR-1253, and interleukin 6 (IL-6) were conducted via a quantitative real-time polymerase chain reaction (qPCR) approach
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Identification of a Novel Prognostic Lymphangiogenesis-Related Signature Associated with Tumor Immunity for Guiding Therapy in Lung Adenocarcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-2-17 Juan Peng, Dan Liu, Hong-feng Zhang, Qi-hao Hu, Wen Chen, Juan Zou, Juan Zhang, Hui Li, An-bo Gao, Yu-kun Li
Lymphangiogenesis, an integral contributor to lymphatic metastasis, is a significant reason for the poor prognosis of cancer patients. Anti-lymphangiogenesis treatment is a promising novel therapeutic direction, especially for tumors resistant to conventional therapies. We confirmed the ectopic expression of lymphangiogenesis-related genes (LRGs) in lung adenocarcinoma (LUAD) cohorts based on the TCGA
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Astragaloside IV Protects against Shear Stress-Induced Glycocalyx Damage and Alleviates Abdominal Aortic Aneurysm by Regulating miR-17-3p/Syndecan-1 Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-2-13 Guojian Li, Qionghui Yang, Kaikai Luo, Ankou Xu, Lijuan Hou, Zhaoxiang Li, Lingjuan Du
Background. The present study aimed to analyze the impact of astragaloside IV (AS-IV) on abdominal aortic aneurysm (AAA) and the glycocalyx, elucidating the potential mechanism of AS-IV. Methods. Rat models of AAA were established using porcine pancreatic elastase. The effects of intraperitoneal AS-IV injection on the morphology, diameter, and glycocalyx of the aorta and the expression of miR-17-3p
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Identification of a 16-MTGs Prognostic Signature in Diffuse Large B-Cell Lymphoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-1-18 Shijun Wang, Xiaoqin Wang, Guixia Li, Pengcheng Feng
Background. Diffuse large B-cell lymphoma (DLBCL) is one of the largest lymphoma subcategories. Usually, 50%–70% of DLBCL patients can be cured by the standard treatment. But, at least one third have bad prognosis. Based on this situation, the research on DLBCL therapy strategy is still indispensable. Methods. A prognostic signature was built according to the public data and bioinformatics methods
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Intraoperative Touch Imprint Cytology of Brain Neoplasms: A Useful High-Diagnostic Tool in 93 Consecutive Cases; Differential Diagnoses, Pitfalls, and Traps Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-1-4 Ali Koyuncuer
Introduction. Intraoperative cytological examination of central nervous system (CNS) lesions was first introduced in 1920 by Eisenhardt and Cushing for rapid evaluation of neurosurgical specimens and to guide surgical treatment. It is recognized that this method not only confirms the adequacy of biopsy in CNS samples but also indicates the presence and preliminary diagnosis of lesional tissue. Methods
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The Clinical and Pathological Effects of Serum C3 Level and Mesangial C3 Intensity in Patients with IgA Nephropathy Anal. Cell. Pathol. (IF 3.2) Pub Date : 2024-1-2 Xiaoyue Hou, Yanan Liang, Weiwei Zhang, Rong Li
Objective. To investigate the clinical and pathological effects of serum C3 level, mesangial C3 deposition intensity and blood lipid on IgA nephropathy. Methods. According to the deposition intensity of immunofluorescence (IF) complement C3 in mesangial region, a total of 151 patients were divided into: (1) negative group (65 cases), (2) weak positive group (51 cases), and (3) strong positive group
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DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-12-20 Rui Hua, Qingjun Chu, Feiyan Guo, Qinjie Chen, Maocai Li, Xuan Zhou, Yongtong Zhu
Background. Nonobstructive azoospermia (NOA) is a complex disease characterized by the spermatogenic dysfunction of testicular tissues. The roles played by long noncoding RNAs (lncRNAs) in NOA pathogenesis have not been extensively studied. Methods. Microarray assays were performed on samples of testicular biopsy tissue obtained from patients with NOA for the purpose of identifying differentially expressed
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Luteolin Pretreatment Ameliorates Myocardial Ischemia/Reperfusion Injury by lncRNA-JPX/miR-146b Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-12-2 Tongda Xu, Yuanyuan Zhang, Gege Liao, Haochen Xuan, Jie Yin, Jieli Bao, Yang Liu, Dongye Li
Background. In the present study, we aimed to find out whether luteolin (Lut) pretreatment could ameliorate myocardial ischemia/reperfusion (I/R) injury by regulating the lncRNA just proximal to XIST (JPX)/microRNA-146b (miR-146b) axis. Methods. We established the models in vitro (HL-1 cells) and in vivo (C57BL/6J mice) to certify the protection mechanism of Lut pretreatment on myocardial I/R injury
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Depletion of lncRNA MEG3 Ameliorates Imatinib-Induced Injury of Cardiomyocytes via Regulating miR-129-5p/HMGB1 Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-11-17 Peng Tang, Jinjian Zhou, Huagang Liu, Shenglan Mei, Kai Wang, Hao Ming
Imatinib is a classical targeted drug to treat chronic myeloid leukemia (CML). However, it shows cardiotoxicity, which limits its clinical application. Long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) shows proapoptotic properties in human cells. This study is performed to investigate whether targeting MEG3 can attenuate imatinib-mediated cardiotoxicity to cardiomyocytes. In this work
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Inhibitory Effect of β-Sitosterol on the Ang II-Induced Proliferation of A7r5 Aortic Smooth Muscle Cells Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-11-7 Yuankun Chen, Shumiao He, Ao Zeng, Siqing He, Xiaobao Jin, Chunmei Li, Wenjie Mei, Qun Lu
Context. Excessive proliferation of vascular smooth muscle cells (VSMCs) plays a critical role in the development of cardiovascular diseases. β-Sitosterol exerts protective effects against the cardiovascular disease. However, whether β-sitosterol protects against the excessive proliferation of VSMCs remain unclear. Objective. To explore the effects of β-sitosterol on VSMC proliferation. Materials and
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METTL16 Inhibits the Malignant Progression of Epithelial Ovarian Cancer through the lncRNA MALAT1/β-Catenin Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-10-28 Changshu Li, Ji Liu, Yuanyuan Lyu, Shizhang Ling, Yonghong Luo
Epithelial ovarian cancer (EOC) ranks third in the incidence of gynecological malignancies. m6A methylation as RNA modification plays a crucial role in the evolution, migration, and invasion of various tumors. However, the role of m6A methylation in ovarian cancer (OC) only recently has begun to be appreciated. Therefore, we used various bioinformatic methods to screen the public GEO datasets of epithelial
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Comprehensive Pan-Cancer Analysis Reveals the Potential Biological, Immunological, and Prognostic Value of NKG2A Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-10-21 Yao Rong, Yongfeng Wang, Mingzheng Tang, Guiqian Zhang, Yuan Yuan, Fengyuan Dong, Zhihang Wu, Guorong Ma, Songhua Liu, Xiashuang Zhao, Hui Cai
Background. NKG2A (KLRC1) belongs to the NKG2 family, which has been shown to affect the activity of natural killer (NK) cells and CD8T cells. However, a comprehensive biological analysis and exploration of NKG2A in different cancers is lacking and this needs to be further investigated. Methods. A comprehensive pan-cancer analysis of NKG2A was performed based on multiple databases. The Cancer Genome
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Low-Density Lipoprotein Contributes to Endometrial Carcinoma Cell Proliferation, Migration, and Invasion by Activating the JAK–STAT Signaling Pathway Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-10-20 Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhu
Background. Cholesterol-rich low-density lipoprotein (LDL) particles have been demonstrated to regulate breast cancer cell proliferation and migration, but their biological function and relevant mechanisms in endometrial carcinoma (EC) remain unclear Methods. Serum and tissue samples were collected from EC patients (n = 50) and patients with benign endometrial hyperplasia (n = 50). Ishikawa and RL95-2
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Comprehensive Analysis of METTLs (METTL1/13/18/21A/23/25/2A/2B/5/6/9) and Associated mRNA Risk Signature in Hepatocellular Carcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-10-12 Haoyu Wang, Shangshang Hu, Junjie Nie, Xiaodan Qin, Xu Zhang, Qian Wang, John Zhong Li
Currently, 80%–90% of liver cancers are hepatocellular carcinomas (HCC). HCC patients develop insidiously and have an inferior prognosis. The methyltransferase-like (METTL) family principal members are strongly associated with epigenetic and tumor progression. The present study mainly analyzed the value of METTLs (METTL1/13/18/21A/23/25/2A/2B/5/6/9) and associated mRNA risk signature for HCC. METTLs
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Machine Learning Developed a Programmed Cell Death Signature for Predicting Prognosis, Ecosystem, and Drug Sensitivity in Ovarian Cancer Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-10-11 Le Wang, Xi Chen, Lei Song, Hua Zou
Background. Ovarian cancer (OC) is the leading cause of gynecological cancer death and the fifth most common cause of cancer-related death in women in America. Programmed cell death played a vital role in tumor progression and immunotherapy response in cancer. Methods. The prognostic cell death signature (CDS) was constructed with an integrative machine learning procedure, including 10 methods, using
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miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading Keap1 Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-10-3 Wei Geng, Shaohua Yan, Xinyue Li, Qiumei Liu, Xuefei Zhang, Xinshun Gu, Xiang Tian, Yunfa Jiang
Early reperfusion into the myocardium after ischemia causes myocardial ischemia–reperfusion (I/R) injury and ferroptosis was involved. Ischemia activates the expression of a series of oxidative stress genes and their downstream regulatory genes, including ferroptosis-related genes such as nuclear factor E2-related factor 2 (Nrf2), glutathione peroxidase 4 (GPX4), and SLC7A11. This study adopted primary
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Selenium Donor Inhibited Hepatitis B Virus Associated Hepatotoxicity via the Apoptosis and Ferroptosis Pathways Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-8-30 Jingdong Shi, Zhen Liu, Weina Li, Di Wang
Background and Aim. Selenium possesses anticancer and antiviral properties, but its mechanism of action remains unclear. Especially, the impact of selenium on hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) remains unknown. This study aimed to investigate the inhibitory properties of selenium on HBV replication and its related hepatotoxicity via the apoptosis and ferroptosis pathways
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Overexpression of Bruton Tyrosine Kinase Inhibits the Proliferation, Migration, and Invasion of Non-Small Cell Lung Cancer Cells Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-8-18 Wenjia Ren, Cheng Yue, Linjun Liu, Licheng Du, Ke Xu, Yubai Zhou
Lung cancer is one of the most lethal malignant tumors in the world. Non-small cell lung cancer (NSCLC) is the most common pathological subtype. However, the molecular mechanism of NSCLC progress is still unclear. We extracted the expression data of the Bruton’s tyrosine kinase (BTK) gene in NSCLC tissues from the TCGA database. The results of paired t-test showed that the BTK gene was significantly
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Mesenchymal Stem Cell-Derived Exosomal miRNA-222-3p Increases Th1/Th2 Ratio and Promotes Apoptosis of Acute Myeloid Leukemia Cells Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-8-16 Yuan Yuan, Shengfen Tan, Huanhuan Wang, Junfeng Zhu, Jiajia Li, Pingping Zhang, Meng Wang, Feng Zhang
Interferon regulatory factor 2 (IRF2) participates in the differentiation of immune T cells. Bone marrow mesenchymal stem cell (BM-MSC)-derived exosomes can secret mRNA, miRNAs, and proteins to regulate tumor microenvironment. The present study focused on the miRNA/IRF2 axis in regulating Th1/Th2 ratio and cell apoptosis in acute myeloid leukemia (AML). The flow cytometry analysis was performed to
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Brain Renin–Angiotensin System: From Physiology to Pathology in Neuronal Complications Induced by SARS-CoV-2 Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-8-4 Shamseddin Ahmadi, Shiler Khaledi
Angiotensin-converting enzyme 2 (ACE2), a key enzyme in the renin–angiotensin system (RAS), is expressed in various tissues and organs, including the central nervous system (CNS). The spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for coronavirus disease-2019 (COVID-19), binds to ACE2, which raises concerns about the potential for viral infection
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MicroRNA-17-5p and MicroRNA-130b-3p Promote Radioresistance of Glioma Stem Cells by Targeting PTEN/AKT/HIF-1α Pathway-Controlled Phosphopentose Metabolism Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-8-4 Tao Xie, Yu-Hao Ding, Jun Dong, Xi-An Fu
Background. The radioresistance of glioma stem cells (GSCs) is related to some microRNAs (miRs) generated by radiation. This study aimed to investigate the effects of miR-17-5p and miR-130b-3p on the radiosensitivity of GSCs. Methods. miR-17-5p and miR-130b-3p expressions in SU3 and SU3-5R cells were determined. SU3 cells transfected with miR-17-5p or miR-130b-3p mimics or inhibitors were used to determine
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miRNA326-5p Targets DKC1 Gene to Regulate Apoptosis-Related Proteins and Intervene in the Development of Neuroblastoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-7-1 Xiao-Hui Wang, Shu-Feng Zhang, Hai-Ying Wu, Jian Gao, Lin Wang, Xu-Hui Wang, Tian-Hui Gao
Objective. To study the effect of congenital dyskeratosis 1 (DKC1) on neuroblastoma and its regulation mechanism. Methods. The expression of DKC1 in neuroblastoma was analyzed by TCGA database and molecular assay. NB cells were transfected with siDKC1 to observe the effects of DKC1 on proliferation, cloning, metastasis, and invasion, and apoptosis and apoptosis-related proteins. The tumor-bearing mouse
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Functional Role of RBP in Osteosarcoma: Regulatory Mechanism and Clinical Therapy Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-6-30 Ziyuan Que, Kang Yang, Nan Wang, Shuying Li, Tao Li
Malignant bone neoplasms can be represented by osteosarcoma (OS), which accounts for 36% of all sarcomas. To reduce tumor malignancy, extensive efforts have been devoted to find an ideal target from numerous candidates, among which RNA-binding proteins (RBPs) have shown their unparalleled competitiveness. With the special structure of RNA-binding domains, RBPs have the potential to establish relationships
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Effect of PTGES3 on the Prognosis and Immune Regulation in Lung Adenocarcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-6-28 Wenyan Jiang, Qiong Wei, Haiqin Xie, Dandan Wu, Haiyan He, Xuedong Lv
Background. PTGES3 is upregulated in multiple cancer types and promotes tumorigenesis and progression. However, the clinical outcome and immune regulation of PTGES3 in lung adenocarcinoma (LUAD) are not fully understood. This study aimed to explore the expression level and prognostic value of PTGES3 and its correlation with potential immunotherapy in LUAD. Methods. All data were obtained from several
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LncRNA AC040162.3 Promotes HCV-Induced T2DM Deterioration through the miRNA-223-3p/NLRP3 Molecular Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-6-17 Ben Niu, Xueshan Xia, Lijing Ma, Lixuan Yao, Yating Zhang, Heng Su
Background. Diabetes is one of the most common diseases and major public health burdens worldwide. Type 2 diabetes mellitus (T2DM) is associated with chronic hepatitis C virus (HCV) infection, and lncRNAs play an important role in HCV-induced T2DM. We aimed to explore the effect of lncRNA AC040162.3 on HCV-induced T2DM. Methods. HCV was used to infect MIN6 cells to establish an in vitro model. HCV
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Diagnostic Accuracy of p16INK4a/Ki-67 Dual Immunostaining for Detection of High-Grade Cervical Intraepithelial Neoplasia in Women Involved in Cervical Cancer Screening in Georgia Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-6-5 Sopio Kakaliashvili-Dzagnidze, Omar Khardzeishvili, Sergo Tabagari
Background. Despite the widespread introduction of primary and secondary preventative measures, death rates for cervical cancer are still significantly high among females, especially in developing countries. Pap cytology and human papillomavirus-based screening often lead to unnecessary additional testing. The aim of this study is to analyze diagnostic accuracy of p16INK4a/Ki-67 dual immunostaining
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TMEM147 Correlates with Immune Infiltration and Serve as a Potential Prognostic Biomarker in Hepatocellular Carcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-6-3 Sheng Cheng, Jutang Li, Ming Xu, Qun Bao, Jiaoxiang Wu, Peng Sun, Bo Han
Background. Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and is associated with high mortality. Transmembrane protein 147 (TMEM147) is a seven-transmembrane protein that may mediate immune regulation. However, the relevance of TMEM147 to immune regulation in HCC and the prognosis of HCC patients are unclear. Methods. We analyzed TMEM147 expression in HCC by using the Wilcoxon
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LINC01614 Promotes Colorectal Cancer Cell Growth and Migration by Regulating miR-217-5p/HMGA1 Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-5-31 Jiwei Jia, Pei Guo, Li Zhang, Wenqing Kong, Fangfang Wang
Colorectal cancer (CRC) substantially contributes to cancer-related deaths worldwide. Recently, a long non-coding RNA (lncRNA), LINC01614, has emerged as a vital gene regulator in cancer progression. Yet, how LINC01614 affects CRC progression remains enigmatic. Here, we defined LINC01614 expression in CRC, investigated the performance of CRC cells lacking LINC01614, and elucidated the underpinning
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Construction of a Cuproptosis-Related lncRNA Prognostic Model for Bladder Urothelial Carcinoma and Screening of Potential Drugs Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-5-25 Xuzhan Ma, Libin Sun
Objective. This study aimed to analyze the cuproptosis-related long non-coding RNA (lncRNA) in patients with bladder urothelial carcinoma (BLCA), construct a prognostic model, and screen its potential drugs. Methods. The transcriptome expression and clinical and mutation burden data related to BLCA were downloaded from The Cancer Genome Atlas database. The prognostic lncRNAs were screened using univariate
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LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-5-17 Bin Chen, Xin Xu, Wei Wu, Ke Zheng, Yijun Yu
Hepatocellular carcinoma (HCC) is a malignant type of liver cancer that poses severe threat to human health worldwide. Aerobic glycolysis is a hallmark of HCC and facilitates its progression. Solute carrier family 10 member 1 (SLC10A1) and long intergenic non-protein coding RNA 659 (LINC00659) were detected to be downregulated in HCC cells, yet their potential functions underlying HCC progression remained
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Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-5-8 Pei-Long Liu, Jia-Yu Diao, Qiong Wang, Huan Liu, Yan Zhang, Jing-Qi Liang, Feng Zhang, Xiao-Jun Liang, Hong-Mou Zhao
Background. Diabetic neuropathic osteoarthropathy (DNOAP) is a rare and easily missed complication for diabetes that leads to increased morbidity and mortality. DNOAP is characterized by progressive destruction of bone and joint, but its pathogenesis remains elusive. We herein aimed to investigate the pathological features and pathogenesis of the cartilages damage in DNOAP patients. Methods. The articular
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Combined Use of Cell Block and Smear Improves the Cytological Diagnosis of Malignancy in Non-Palpable Breast Lesions Screened by Imaging Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-5-3 Rieko Nishimura, Mikinao Oiwa
Background. Currently, core needle biopsy is replacing fine needle aspiration biopsy (FNAB) for pathological diagnosis of breast lesions. However, FNAB is extensively used for diagnosing breast lesions, including screened lesions, at our hospital. Furthermore, direct smears as well as cell blocks (CBs) from the FNAB specimens have been used. To prepare the CBs, hematoxylin and eosin (HE) staining as
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The Accurate Interpretation and Clinical Significance of Morphological Features of Fine Needle Aspiration Cells in Papillary Thyroid Carcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-5-3 Xue-Jiao Xiong, Ming-Ming Xiao, Yi-Xia Zhang, Dong-Ge Liu, Mu-Lan Jin, Jian Wang, Hong-Tao Xu, Qing-Chang Li, Guang-Ping Wu
Papillary thyroid carcinoma (PTC) is the most common malignant neoplasm of the thyroid gland; fine needle aspiration cytology is the most basic and reliable diagnostic method before PTC operation. However, it is not clear which cell morphological changes can be used as a reliable standard for the diagnosis of PTC. A retrospective analysis was performed on 337 patients with PTC confirmed by postoperative
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Comparative Effect of the Active Substance of Thyme with N-Acetyl Cysteine on Hematological Parameters and Histopathological Changes of Bone Marrow and Liver in Rat Models of Acetaminophen Toxicity Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-4-4 Zahra Mokhtari, Mahdieh Raeeszadeh, Loghman Akradi
Acetaminophen has always been at the center of attention as a non-steroidal anti-inflammatory drug, which is generally associated with the serious side effects on liver and the hematological parameters. This study aimed to compare the effect of N-acetyl cysteine (NAC) and thyme extract on rat models of acetaminophen-induced toxicity. The present experimental study was conducted on 48 Wistar rats randomized
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Bioinformatics Analysis of Molecular Interactions between Endoplasmic Reticulum Stress and Ferroptosis under Stress Exposure Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-3-30 Weihao Zhu, Yingmin Li, Meili Li, Jingmin Liu, Guowei Zhang, Xiaoying Ma, Weibo Shi, Bin Cong
Stress has become a universal biological phenomenon in the body, which leads to pathophysiological changes. However, the molecular network interactions between endoplasmic reticulum (ER) stress and ferroptosis under stressful conditions are not clear. For this purpose, we screened the gene expression profile of GSE173795 for intersection with ferroptosis genes and screened 68 differentially expressed
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-3-29 Yang Sun, Sanmao Su, Mengjun Li, Ang Deng
Background. The pathogenesis of osteoarthritis (OA) is complex and there is no specific drug for treatment. The aim of this study was to identify the molecular targets of OA therapy, focusing on the expression and biological functions of miR-182-5p and its target genes in OA. Methods. miR-182-5p and fibroblast growth factor 9 (FGF9) were overexpressed or knocked down in IL-1β-induced chondrocytes.
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Effect of COP1 in Promoting the Tumorigenesis of Gastric Cancer by Down-Regulation of CDH18 via PI3K/AKT Signal Pathway Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-3-28 Benhuo Zhao, Jiaojiao Wu, Xiuli Cha, Guangtong Mao, Hengliang Shi, Sujuan Fei, Bei Miao
In recent years, the involvement of E3 ubiquitin ligase constitutive photomorphogenesis 1 (COP1) in the tumorigenesis of gastric cancer (GC) has been elucidated. However, the exact underlying mechanism remains to be clarified. In the present study, the expression profiles of COP1 in GC were derived from the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) databases, followed by verification
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Classification of Signature-Based Phenotypes of Aging-Related Genes to Identify Prognostic and Immune Characteristics in HCC Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-3-20 Junjie Zhao, Chong Li, Qinggang Li, Shen Shen, Xiaobo Hu, Zihui Dong, Yize Zhang, Jiyuan Xing
Hepatocellular carcinoma (HCC), which has become one of the most significant malignancies causing cancer-related mortality, presents genetic and phenotypic heterogeneity that makes predicting prognosis challenging. Aging-related genes have been increasingly reported as significant risk factors for many kinds of malignancies, including HCC. In this study, we comprehensively dissected the features of
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Suppression of lncRNA OIP5-AS1 Attenuates Apoptosis and Inflammation, and Promotes Proliferation by Mediating miR-25-3p Expression in Lipopolysaccharide-Induced Myocardial Injury Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-3-20 Jiaju Ma, Hebu Qian, Han Zou
Purpose. Long non-coding RNAs (LncRNAs) OIP5-AS1 and miR-25-3p play important roles in myocardial injury, whereas their roles in lipopolysaccharide (LPS)-induced myocardial injury remain unknown. The purpose of our study was to investigate the functional mechanisms of OIP5-AS1 and miR-25-3p in LPS-induced myocardial injury. Methods. Rats and H9C2 cells were treated with LPS to establish the model of
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The Protective Role of Nrf2 in Renal Tubular Cells in Oxidised Low-Density Lipoprotein-Induced Fibrosis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-3-11 Xiangju Long, Zhe Liu, Yanan Sun, Hong Zhang
Background: CD36 is the receptor of oxidised low-density lipoprotein (OxLDL) in renal tubular epithelial cells. Nuclear factor erythroid 2-related factor 2 (Nrf2) is the key factor in the activation of the Nrf2 signalling pathway and the regulation of oxidative stress. Kelch-like ECH-associated protein 1 (Keap1) is known as an Nrf2 inhibitor. Methods: We used OxLDL and Nrf2 inhibitors at different
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Association between CHFR and PARP-1, and Their Roles in Regulation of Proliferation and Apoptosis of B Cell Lymphoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-2-28 Xiao-Dan Liu, Nian-Ju Zheng, Liang Song, Hua Pan, Ai-Qin Song
Background. Aberrant methylation of checkpoint with forkhead and ring finger domains (CHFR) was found in B-cell non-Hodgkin lymphoma (NHL), whereas its role in carcinogenesis is not clear. CHFR can control poly (ADP-ribose) polymerase levels by causing its degradation. The study was aimed to explore the roles and mechanisms of CHFR in the pathogenesis of B-cell NHL. Methods. Short hairpin ribonucleic
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Mechanism of Action of Decitabine in the Treatment of Acute Myeloid Leukemia by Regulating LINC00599 Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-2-22 Fan Du, Ting Jin, Li Wang
Objective. Acute myeloid leukemia (AML) is a heterogeneous malignancy with a low long-term survival rate. The aim of this study was to investigate the effects of decitabine (DAC) treatment cell proliferation and apoptosis in AML and role of the expression of LINC00599 and, consequently, miR-135a-5p. Materials and Methods. Human promyelocytic leukemia cells (HL-60) and human acute lymphatic leukemia
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Nicotine Suppresses Phagocytic Ability of Macrophages by Regulating the miR-296-3p–SIRPα Axis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-2-15 Zhen Liu, Fang Wang, Xiaowu Huang, Zhi Chen, Yicheng Zhao, Yawei Wang, Xiaobo Luo, Guanren Zhao
Phagocytic ability of macrophage is responsible for tuberculosis infection. Nicotine has been shown to attenuate the phagocytic ability of macrophage; however, the underlying mechanism remains unclear. Here, we demonstrated that nicotine increased the message RNA (mRNA) and protein expression of signal regulatory protein alpha (SIRPα) and enhanced the stability of SIRPα mRNA in macrophage. Nicotine
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Interleukin-6 and Hypoxia Synergistically Promote EMT-Mediated Invasion in Epithelial Ovarian Cancer via the IL-6/STAT3/HIF-1α Feedback Loop Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-2-13 Tongshuo Zhang, Jing Yang, Yang Sun, Jiangnan Song, Dandan Gao, Suhui Huang, Aibo Pang, Jianhui Zhang, Junhong Wang, Yue Wang, Yanqiu Li
Extensive peritoneal spread and capacity for distant metastasis account for the majority of mortality from epithelial ovarian cancer (EOC). Accumulating evidence shows that interleukin-6 (IL-6) promotes tumor invasion and migration in EOC, although the molecular mechanisms remain to be fully elucidated. Meanwhile, the hypoxic microenvironment has been recognized to cause metastasis by triggering e
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Hypoxia-Induced GST1 Exerts Protective Effects on Trophoblasts via Inhibiting Reactive Oxygen Species (ROS) Accumulation Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-1-25 Lingjuan Chen, Gaoli Chen, Lixuan Guo, Yaping Wang, Chengjin Ai
Hypoxic conditions are a typical extrinsic factor for the modification of trophoblast biological functions, including cell proliferation, migration, and invasion. Hypoxia-induced reactive oxygen species (ROS) accumulation causes chronic trophoblast injury and contributes to preeclampsia (PE). Glutathione-S-transferase P (GSTP1) is a main regulator of ROS. However, it is still unknown whether GSTP1
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Development of a Novel KCNN4-Related ceRNA Network and Prognostic Model for Renal Clear Cell Carcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-1-24 Hengtao Bu, Qiang Song, Jiexiu Zhang, Yuang Wei, Bianjiang Liu
Background. Clear cell renal cell carcinoma (ccRCC) accounts for more than 80% of renal cell carcinomas. Yet, it has not been fully understood about the derivation and progression of the tumor, as well as the long-term benefits from multimodality therapy. Therefore, reliable and applicable molecular markers are urgently needed for the prediction of diagnosis and prognosis of ccRCC patients. Methods
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Incidental Papillary Microcarcinoma and Papillary Thyroid Carcinoma in Multinodular Goiter Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-1-14 Cigdem D. Arican, Tulin Ozturk, Muhammet Sait Sager, Ipek Sertbudak, Serkan Teksoz, Cansu Turker Saricoban, Abdulkerim Uygur
Introduction. This study aimed to examine the incidence of incidental papillary microcarcinoma (PMC) and papillary thyroid carcinoma (PTC) in patients with benign multinodular goiter (MNG) and to compare their relationship with some prognostic factors from a new perspective. Methods. Bilateral total thyroidectomy (BTT) was used to evaluate the data of 716 patients who underwent a surgery for MNG. The
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A-Kinase Anchor Protein 95 Is Involved in ERK1/2–Elk-1 Signal Transduction in Colon Cancer Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-1-14 Xiangyu Kong, Putian An, Junping Xu, Wenzhi Liu, Feng Lin, Yulong Yang
Objectives. To assess A-kinase anchor protein 95 (AKAP95), B-Raf, extracellular regulated protein kinases 1/2 (ERK1/2), and Elk-1 expression in colon cancer tissue, and characterize AKAP95 associations with B-Raf, ERK1/2, Elk-1, and colon cancer clinicopathological indices. Methods. The immunohistochemistry streptavidin-perosidase (SP) method was used to determine protein expression levels in 64 colon
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PCSK9-D374Y Suppresses Hepatocyte Migration through Downregulating Free Cholesterol Efflux Rate and Activity of Extracellular Signal-Regulated Kinase Anal. Cell. Pathol. (IF 3.2) Pub Date : 2023-1-9 Lei Huang, Ying Cheng, Yulian Mu, Kui Li
Proprotein convertase subtilisin/kexin type 9 can mediate the intracellular lysosomal degradation of the low-density lipoprotein receptor protein in hepatocytes and decrease the liver’s ability to scavenge low-density lipoprotein cholesterol from circulation, resulting in high levels of cholesterol in the circulatory system. Current studies have primarily focused on the relationship between PCSK9 and
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Identification of Differentially Expressed Genes Particularly Associated with Immunity in Uremia Patients by Bioinformatic Analysis Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-12-29 Guixia Li, Shijun Wang, Jing Huo
Uremia is a common syndrome that happens to nearly all end-stage kidney diseases, which profound have changes in human gene expressions, but the related pathways are poorly understood. Gene Ontology categories and Kyoto Encyclopedia of Genes and Genomes pathways enriched in the differentially expressed genes (DEGs) were analyzed by using clusterProfiler, org.Hs.eg.db, and Pathview, and protein–protein
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Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-12-26 Haixia Deng, Zhanhong Tang, Peng Tuo, Ruihua Wu, Si Jia, Xuan Zhao, Deqing Huang, Yuguang Gao, Zhou Lan
The effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sham group, model group, conventional western medicine (CWM) group, Shenfu group, and antagonist group
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CDCA2 Promotes HCC Cells Development via AKT–mTOR Pathway Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-12-22 Kai Li, Tingting Fan, Zhongxing Shi, Huijie Jiang
Background. Hepatocellular carcinoma (HCC) is a highly aggressive and solid malignancy with a poor prognosis. Cell division cycle associated 2 (CDCA2) is highly expressed in HCC and is considered to be closely related to the prognosis of patients with HCC. In this research, we aimed to investigate the function and potential mechanism of CDCA2 in HCC cells. Methods. Gain- and loss-of-function experiments
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A Cuproptosis-Related lncRNAs Signature Could Accurately Predict Prognosis in Patients with Clear Cell Renal Cell Carcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-12-22 Wei Zhang, Han Wang, Wei Wang, Haoqiang Xue, Maolin Qiao, Liying Song, Shuang Wang, Zhaoyu Ren, Zhifang Ma
Background. Clear cell renal cell carcinoma (ccRCC) is the most common subtype of kidney cancers. As cuproptosis, a new cell death mechanism proposed recently, differs from all other known mechanisms regulating cell death, we aimed to create prognostic markers using cuproptosis-related long non-coding ribonucleic acids (RNAs; lncRNAs) and elucidate the molecular mechanism. Methods. Data from transcriptome
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Clinical Significance of TUBGCP4 Expression in Hepatocellular Carcinoma Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-12-8 Chuanjun Zheng, Jiaxi Zhang, Fusheng Jiang, Di Li, Caimei Huang, Xuefeng Guo, Xiaonian Zhu, Shengkui Tan
We aim to investigate the expression and clinical significance of the tubulin gamma complex-associated protein 4 (TUBGCP4) in hepatocellular carcinoma (HCC). The mRNA expression of TUBGCP4 in HCC tissues was analyzed using The Cancer Genome Atlas (TCGA) database. Paired HCC and adjacent nontumor tissues were obtained from HCC patients to measure the protein expression of TUBGCP4 by immunohistochemistry
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Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-11-22 Jing Xu, Jin Huang, Xiaojie He, Mingshuang Hu, Shan Su, Ping Liu
Myocardial ischemia/reperfusion (I/R) injury seriously threats the health and life of patients with ischemia heart disease. Herein, we probed the potential influence of myosin 1b (myo1b) on hypoxia/reoxygenation- (H/R-) stimulated cardiomyocyte H9c2 cell apoptosis and autophagy. After H/R stimulation, the myo1b mRNA level in H9c2 cells was tested via qRT-PCR. Myo1b overexpression plasmid (OE-myo1b)
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Model of Liver Fibrosis Induction by Thioacetamide in Rats for Regenerative Therapy Studies Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-11-12 Nathaly Enciso, José Amiel, Fredy Fabián-Domínguez, Jhon Pando, Nancy Rojas, Carlos Cisneros-Huamaní, Ernesto Nava, Javier Enciso
Hepatic fibrosis is caused by chronic injury due to toxic, infectious, or metabolic causes, and it may progress to cirrhosis and hepatocellular carcinoma. There is currently no antifibrotic therapy authorized for human use; however, there are promising studies using cell therapies. There are also no animal models that exactly reproduce human liver fibrosis that can be used to better understand the
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Monocarboxylate Transporters: Role and Regulation in Corneal Diabetes Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-10-26 Pawan Shrestha, Amy E. Whelchel, Sarah E. Nicholas, Wentao Liang, Jian-Xing Ma, Dimitrios Karamichos
Diabetes mellitus (DM) is a group of metabolic diseases that is known to cause structural and functional ocular complications. In the human cornea, DM-related complications affect the epithelium, stroma, and nerves. Monocarboxylate transporters (MCTs) are a family of proton-linked plasma membrane transporters that carry monocarboxylates across plasma membranes. In the context of corneal health and
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Transcription Factor FXR Activates DHRS9 to Inhibit the Cell Oxidative Phosphorylation and Suppress Colon Cancer Progression Anal. Cell. Pathol. (IF 3.2) Pub Date : 2022-10-26 Jinlai Zhao, Yigang Wang, Yang Wang, Jianchao Gao, Haichao Yang, Xiaotang Wu, Hua Li
Background. Colon cancer is a common gastrointestinal malignancy. It has been discovered that Farnesoid X receptor (FXR) plays an imperative regulatory role in multitype cancers in recent years. However, its regulatory mechanism in colon cancer has not been clearly explored. This study intended to explore the molecular regulatory mechanism of FXR and its downstream genes on the malignant progression