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Validated differentiation of Listeria monocytogenes serogroups by FTIR spectroscopy using an Artificial Neural Network based classifier in an accredited official food control laboratory Clin. Spectrosc. Pub Date : 2023-10-27 Helene Oberreuter, Martin Dyk, Jörg Rau
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Rapid detection of heart failure using a spectroscopic liquid biopsy Clin. Spectrosc. Pub Date : 2023-10-21 Loren Christie, Alexandra Sala, James M. Cameron, Justin J.A. Conn, David S. Palmer, William J. McGeown, Jane A. Cannon, John Sharp, Matthew J. Baker
Heart disease is growing annually across the globe with numbers expected to rise to 46% of the population by 2030. Early detection is vital for several reasons, firstly it improves the long-term prognosis of the patient by admitting them through the appropriate pathway faster, secondly it reduces healthcare costs by streamlining diagnosis and finally, in combination with management or treatment, it
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Application of optical spectroscopy in diagnosing and monitoring breast cancers: A technical review Clin. Spectrosc. Pub Date : 2023-10-07 Afshan Shirkavand, Mozhdeh Babadi, Leila Ataie Fashtami, Ezeddin Mohajerani
Breast cancer is one of the most prevalent cancers among the global women population. It is due to the development of malignant cells in the breast tissue based on external or internal causes. The stages of detecting breast cancer include screening, diagnosis, and prognosis. Multiple imaging modalities, including digital Mammography, Ultrasonography, breast MRI, CT scan, and PET are applied for screening
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Raman spectroscopic analysis of human serum samples of convalescing COVID-19 positive patients Clin. Spectrosc. Pub Date : 2023-10-07 Naomi Jackson, Jaythoon Hassan, Hugh J. Byrne
Rapid screening, detection and monitoring of viral infection is of critical importance, as exemplified by the rapid spread of SARS-CoV-2, leading to the worldwide pandemic of COVID-19. This is equally the case for the stages of patient convalescence as for the initial stages of infection, to understand the medium and long terms effects, as well as the efficacy of therapeutic interventions. Optical
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Deep UV resonance Raman spectroscopy for sensitive detection and quantification of the fluoroquinolone antibiotic drug moxifloxacin and the β-lactam meropenem in human plasma Clin. Spectrosc. Pub Date : 2023-09-26 Christian Domes, Juergen Popp, Stefan Hagel, Mathias W. Pletz, Torsten Frosch
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Detection of several respiratory viruses with Surface-Enhanced Raman Spectroscopy coupled with Artificial Intelligence Clin. Spectrosc. Pub Date : 2023-07-12
Diagnoses of viral infections are a challenge when facing a crisis like COVID-19, where their speed and reliability are critical to minimize diseases spread. The gold standard of diagnostics, quantitative Polymerase Chain Reaction, is time- and reagent-consuming and requires qualified personnel. Therefore, it is necessary to find new detection techniques to overcome these barriers. Surface Enhanced
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Biochemical characterization and discrimination of Leishmania major parasites and infected macrophages with Raman spectroscopy and chemometrics Clin. Spectrosc. Pub Date : 2023-06-07 Thulya Chakkumpulakkal Puthan Veettil, Rebekah N. Duffin, Supti Roy, Philip C. Andrews, Bayden R. Wood
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Contributions of vibrational spectroscopy to virology: A review Clin. Spectrosc. Pub Date : 2022-05-11 Iqra Chaudhary, Naomi Jackson, Denise Denning, Luke O’Neill, Hugh J. Byrne
Vibrational spectroscopic techniques, both infrared absorption and Raman scattering, are high precision, label free analytical techniques which have found applications in fields as diverse as analytical chemistry, pharmacology, forensics and archeometrics and, in recent times, have attracted increasing attention for biomedical applications. As analytical techniques, they have been applied to the characterisation
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Improved tissue preparation for multimodal vibrational imaging of biological tissues Clin. Spectrosc. Pub Date : 2022-02-18 Callum Gassner, John A. Adegoke, Sheila K. Patel, Varun J. Sharma, Kamila Kochan, Louise M. Burrell, Jaishankar Raman, Bayden R. Wood
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An observational cohort study to evaluate the use of serum Raman spectroscopy in a rapid diagnosis center setting Clin. Spectrosc. Pub Date : 2022-01-20 Freya E.R. Woods, Susan Chandler, Natalia Sikora, Rachel Harford, Ahmad Souriti, Helen Gray, Heather Wilkes, Catherine Lloyd-Bennett, Dean A. Harris, Peter R. Dunstan
Cancer presenting with non-specific vague symptoms remains a clinical challenge. The purpose of this study was to assess the feasibility of serum Raman spectroscopy for cancer detection in a rapid diagnosis center (RDC) setting. The primary aim was to identify significant spectral peaks of change in sera from cancer patients and the secondary aim was to assign molecular species at Raman peaks. In this
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“A spectroscopic picture paints 1000 words” mapping iron speciation in brain tissue with “full spectrum per pixel” X-ray absorption near-edge structure spectroscopy Clin. Spectrosc. Pub Date : 2021-10-14 Mark J. Hackett, Gaewyn Ellison, Ashley Hollings, Frederick Colbourne, Martin D. de Jonge, Daryl L. Howard
Coordination chemistry enables a variety of vital functions in biological systems; however, characterising the chemical form of metal ions in cells and tissue is notoriously difficult. One technique that is gaining substantial momentum in this research area is X-ray absorption near-edge structure (XANES) spectroscopy. The XANES spectrum can be a rich source of information with respect to the coordination
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Detection of cancer markers or marker surrogates by infrared spectral histopathology (SHP): PD-L1 and Her2/neu Clin. Spectrosc. Pub Date : 2021-10-15 Max Diem
In this contribution, data will be presented that suggest that certain cancer markers or their surrogates can be detected by infrared spectral imaging methodology. In particular, the co-localization of certain regions in spectral images with PD-L1-postive immunohistochemical response suggests that either the PD-L1 protein or a surrogate associated with its presence are detectable. In the case of Her2/neu
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Could Raman spectroscopy distinguish between P. falciparum and P. vivax Infection? Clin. Spectrosc. Pub Date : 2021-09-29 Malwina Birczyńska-Zych, Jacek Czepiel, Maria Łabanowska, Martyna Kraińska, Grażyna Biesiada, Paulina Moskal, Mateusz Kozicki, Aleksander Garlicki, Aleksandra Wesełucha-Birczyńska
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Spectroscopic screening of pancreatic cancer Clin. Spectrosc. Pub Date : 2021-09-27 Krzysztof Szymoński, Ewelina Lipiec, Kamila Sofińska, Katarzyna Skirlińska-Nosek, Katarzyna Milian-Ciesielska, Joanna Szpor, Michał Czaja, Sara Seweryn, Natalia Wilkosz, Giovanni Birarda, Federica Piccirilli, Lisa Vaccari, Marek Szymoński
Pancreatic cancer is one of the most aggressive and lethal malignant neoplasms in the world and 5-year survival rate still remains below 10%. Although, there are many reasons for that, one definitely is the lack of early and unique diagnostic tools for pancreatic cancer screening, which somehow correlates with no sufficient knowledge about cancer’s molecular nature. This article aims to prove the concept
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Spatially resolved macromolecular orientation in biological tissues using FT-IR imaging Clin. Spectrosc. Pub Date : 2021-07-31 Karolina Kosowska, Paulina Koziol, Danuta Liberda, Tomasz P. Wrobel
Fourier-transform infrared spectroscopy (FT-IR) in combination with machine learning and chemometrics is an intensively developed, powerful tool for investigation of tissue biochemical composition with simultaneous microscopic visualization. This nondestructive, information rich and label-free technique has been successfully applied in cancer diagnostics. During the development of a disease or inflammatory
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Spectral artefacts induced by moving targets in live hyperspectral stimulated Raman spectroscopy: The case of lipid droplets in yeast cells Clin. Spectrosc. Pub Date : 2021-08-03 Cassio Lima, Chrispian W. Theron, Howbeer Muhamadali, Douglas B. Kell, Royston Goodacre
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Intra-operative Raman spectroscopy and ex vivo Raman mapping for assessment of cartilage degradation Clin. Spectrosc. Pub Date : 2021-06-07 Riana Gaifulina, Abigail D.G. Nunn, Edward R.C. Draper, Robin K. Strachan, Nathan Blake, Steven Firth, Geraint M.H. Thomas, Paul F. McMillan, Jayesh Dudhia
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Hyperspectral characterization of the MSTO-211H cell spheroid model: A FPA–FTIR imaging approach Clin. Spectrosc. Pub Date : 2021-05-26 Valentina Notarstefano, Simona Sabbatini, Maurizio Sabbatini, Aldo Arrais, Alessia Belloni, Chiara Pro, Lisa Vaccari, Domenico Osella, Elisabetta Giorgini
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Raman spectroscopic characterisation of non stimulated and stimulated human whole saliva Clin. Spectrosc. Pub Date : 2021-05-25 Genecy Calado, Isha Behl, Hugh J. Byrne, Fiona M. Lyng
Human saliva is a unique biofluid which can reflect the physiopathological state of an individual. The wide spectrum of molecules present in saliva, compounded by the close association of salivary composition to serum metabolites, can provide valuable information for clinical diagnostic applications through highly sensitive vibrational spectroscopic techniques such as Raman spectroscopy. However, the
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A spectroscopic diagnostic for rheumatoid arthritis using liquid biopsies Clin. Spectrosc. Pub Date : 2021-04-14 Neha Chaudhary, Thi Nguyet Que Nguyen, Muddassar Ahmad, Robert Harrington, Caroline A. Jefferies, Grainne Kearns, Aidan D. Meade, Claire Wynne
Rheumatoid arthritis (RA) possess not only a substantial degree of clinical heterogeneity but is diagnosed on a diverse array of clinical criteria. The lack of a single marker predictive methodology means that the timely diagnosis and treatment of these patients proves challenging. With the advent of targeted therapies, it is becoming increasingly important to accurately diagnose RA at an early stage
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Breast cancer detection using infrared spectral pathology from H&E stained tissue on glass slides Clin. Spectrosc. Pub Date : 2021-03-31 Jiayi Tang, Daniela Kurfürstová, Peter Gardner
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Primary murine hepatocytes exposed to fatty acids analyzed by Raman and infrared microscopy Clin. Spectrosc. Pub Date : 2021-03-10 Ewelina Matuszyk, Malgorzata Baranska
Aim of this work is to present the phenotype and biochemical characteristics of primary freshly isolated murine hepatocytes exposed to oleic and palmitic acids, the most abundant fatty acids (FAs) in human diet and serum. Excess of FAs in the diet is one of the factors that contributes to the non-alcoholic fatty liver disease (NAFLD). The hallmark of NAFLD is the overaccumulation of lipids in hepatic
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Breast cancer histopathology using infrared spectroscopic imaging: The impact of instrumental configurations Clin. Spectrosc. Pub Date : 2021-02-27 Shachi Mittal, Tomasz P. Wrobel, Michael Walsh, Andre Kajdacsy-Balla, Rohit Bhargava
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Quantitative analysis of human blood serum using vibrational spectroscopy Clin. Spectrosc. Pub Date : 2020-05-28 Hugh J. Byrne, Franck Bonnier, Jennifer McIntyre, Drishya Rajan Parachalil
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Spectroscopy goes viral: Diagnosis of hepatitis B and C virus infection from human sera using ATR-FTIR spectroscopy Clin. Spectrosc. Pub Date : 2019-12-01 Supti Roy,David Perez-Guaita,Scott Bowden,Philip Heraud,Bayden R. Wood
Abstract The development of a new fast, portable and reagent-free diagnostic technique for hepatitis B (HBV) and hepatitis C (HCV) viruses would be an enormous benefit to society. Here, we evalulate the ability of Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy combined with multivariate data analysis to classify human serum samples based on the presence of HBV and HCV
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Shining a light on clinical spectroscopy: Translation of diagnostic IR, 2D-IR and Raman spectroscopy towards the clinic Clin. Spectrosc. Pub Date : 2019-12-01 Holly J. Butler,James M. Cameron,Cerys A. Jenkins,Gordon Hithell,Samantha Hume,Neil T. Hunt,Matthew J. Baker
Abstract In recent years, the application of vibrational spectroscopy in biomedical research has rapidly expanded; covering aspects of pharmaceutical development, to point-of-care technologies. Vibrational spectroscopy techniques such as Fourier-transform IR (FTIR), and Raman spectroscopy have been at the forefront of this movement, with their complementary information able to shine light onto a range