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Disruption of gonocyte development following neonatal exposure to di (2-ethylhexyl) phthalate Reprod. Biol. (IF 2.1) Pub Date : 2024-03-10 Estefanía Reyes-Cruz, Julio César Rojas-Castañeda, Daniel Adrian Landero-Huerta, Norma Hernández-Jardón, Rafael Reynoso-Robles, María de Lourdes Juárez-Mosqueda, Alfredo Medrano, Rosa María Vigueras-Villaseñor
Pre- and/or post-natal administrations of di(2-ethylhexyl) phthalate (DEHP) in experimental animals cause alterations in the spermatogenesis. However, the mechanism by which DEHP affects fertility is unknown and could be through alterations in the survival and differentiation of the gonocytes. The aim of the present study was to evaluate the effect of a single administration of DEHP in newborn mice
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Circ_0001495 influences the development of endometriosis through the miRNA-34c-5p/E2F3 axis Reprod. Biol. (IF 2.1) Pub Date : 2024-03-07 Yan Yue, Bin Lu, Guantai Ni
Endometriosis is a chronic gynecological condition characterized by the presence of endometrial glands and stroma outside the uterine cavity., accounting for 7% of all female malignant tumors and 20%− 30% of malignant tumors of the female reproductive system. Multiple studies have shown that circular RNA (circRNA) has the potential to become a targeted target and marker for EM. However, the roles of
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Disruption of spermatogenesis in testicular adult Wistar rats after short-term exposure to high dose of glyphosate based-herbicide: Histopathological and biochemical changes Reprod. Biol. (IF 2.1) Pub Date : 2024-02-24 Meriem Hariti, Assia Kamel, Amel Ghozlani, Nacima Djennane, Kamel Djenouhat, Kahina Aksas, Zohra Hamouli-Saïd
Glyphosate is an endocrine disruptor and can act on the activity of certain enzymes of metabolism subsequently altering some functions such as reproduction. The goal of the present study is to evaluate the involvement of glyphosate based-herbicide (GBH) in spermatogenesis disruption and to investigate which cells of the adult Wistar rat testis are most affected by short-term exposure to GBH. Treated
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Gonadotropin upregulates intraovarian calpains-1 and −2 during ovarian follicular recruitment in the SD rat model. Reprod. Biol. (IF 2.1) Pub Date : 2024-02-24 Akanksha Singh, Rupal Tripathi, Rakesh Kumar Gupta, Rumaisa Rashid, Rajesh Kumar Jha
Calpain role has been shown in the cumulus cell-oocyte complexes and, corpus luteum. We investigated the association of calpains-1 and −2 in ovarian folliculogenesis using the Sprague-Dawley (SD) rat model and steroidogenesis in the human granulosa cells (hGCs). We induced PCOS in 42-day-old SD rats by letrozole oral gavage for 21 days. Premature ovarian failure (POF) was induced in 21-day-old SD rats
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The effect of L-carnitine supplementation during in vitro maturation on oocyte maturation and somatic cloned embryo development Reprod. Biol. (IF 2.1) Pub Date : 2024-02-16 Baobao Zhao, Heqiang Li, Han Zhang, Subi Ren, Yuelin Li, Xiaoyan Wang, Xinrui Lan, Hailian Qiao, Huiming Ma, Yong Zhang, Yongsheng Wang
The quality of the recipient cytoplasm was reported as a crucial factor in maintaining the vitality of SCNT embryos and SCNT efficiency for dairy cows. Compared with oocytes matured in vivo, oocytes matured in vitro showed abnormal accumulation and metabolism of cytoplasmic lipids. L-carnitine treatment was found to control fatty acid transport into the mitochondrial β-oxidation pathway, which improved
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The possible regulatory role of miR-514 and miR-642b in cumulus cells on the oocyte maturation in patients with polycystic ovary syndrome Reprod. Biol. (IF 2.1) Pub Date : 2024-02-16 Hanieh Shafienia, Seyed Mehdi Hoseini, Mohammad Ali Khalili, Leila Heydari, Mohammad Hasan Sheikhha, Fateme Montazeri
Polycystic ovary syndrome is a common endocrine disorder in reproductive-age women. Accordingly, abnormal microenvironment may negatively influence oocyte developmental competence as a result of the altered expression profile of cumulus cells (CCs), mainly the key players of oocyte maturation, such as epidermal growth factor receptor (EGFR) and prostaglandin E receptor-2 (PTGER2). This study aimed
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IL-4-induced M2 macrophages inhibit fibrosis of endometrial stromal cells Reprod. Biol. (IF 2.1) Pub Date : 2024-02-14 Dan Feng, Yang Li, Hongyun Zheng, Ying Wang, Juexiao Deng, Tingting Liu, Wenxin Liao, Fujin Shen
Intrauterine adhesions (IUA) refers to endometrial fibrosis caused by irreversible damage of the endometrial basal layer. As the key regulators in tissue repair, regeneration, and fibrosis, macrophages play an essential role in endometrial regeneration and repair during the normal menstrual cycle. However, the mechanism of macrophages involved in IUA remains unclear. In the late stages of proliferation
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Gasdermin D regulates soluble fms-like tyrosine kinase 1 release in macrophages Reprod. Biol. (IF 2.1) Pub Date : 2024-01-30 Hazuki Tanaka, Ren Ozawa, Yuka Henmi, Manabu Hosoda, Tadayoshi Karasawa, Masafumi Takahashi, Hironori Takahashi, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
Preeclampsia (PE) is a serious complication, and soluble fms-like tyrosine kinase (sFLT1) released from the placenta is one of the causes of PE pathology. Trophoblasts are the primary source of sFLT1; however, monocytes/macrophages exist enough in the placenta can also secrete sFLT1. Sterile inflammatory responses, especially NLRP3 inflammasome and its downstream gasdermin D (GSDMD)-regulated pyroptosis
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Altered ovarian tissue level of lysophosphatidic acid and mRNA expressions of its metabolic enzymes and receptors in rats received gonadotropin-hyperstimulation Reprod. Biol. (IF 2.1) Pub Date : 2024-02-01 Aimi Yamamoto-Mikami, Yu Tanaka, Toshihiko Tsutsumi, Akira Kuwahara, Akira Tokumura
Lysophosphatidic acid (LPA), a well-studied member of the lysophospholipid family, is known to exert an important bio-effect on oocyte maturation and ovulation in mammals. We attempted to determine how follicle maturation in the rat ovary affects the levels of LPA and its precursor lysophospholipids, as well as mRNA levels of LPA-producing and -degrading enzymes and LPA receptors in rats that received
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Effects of calorie, protein, and branched chain amino acid restriction on ovarian aging in mice Reprod. Biol. (IF 2.1) Pub Date : 2024-01-30 Gabriel B. Veiga, Bianka M. Zanini, Driele Neske Garcia, Jéssica D. Hense, Mariana M. Barreto, José V.V. Isola, Rafael G. Mondadori, Michal M. Masternak, Michael B. Stout, Augusto Schneider
Calorie restriction (CR) is an intervention that promotes longevity and preserves the ovarian reserve. Some studies have observed that the positive impacts of CR can be linked to restriction of protein (PR) and branched-chain amino acids (BCAAs) independent of calorie intake. The aim of this study was to compare the effects of protein and BCAA restriction to 30% CR on the ovarian reserve of female
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Comparative peptidomics analysis of preeclamptic placenta and the identification of a novel bioactive SERPINA1 C-terminal peptide Reprod. Biol. (IF 2.1) Pub Date : 2024-01-29 Lingling Jiang, Yuanyuan Zhu, Liuxin Wu, Cong Wang, Nana Yang, Yetao Xu, Lizhou Sun
Preeclampsia (PE) is a life-threatening disease that severely harms pregnant women and infants' health but has a poorly understood etiology. Peptidomics can supply important information about the occurrence of diseases. However, application of peptidomics in preeclamptic placentas has never been reported. We conducted a comparative peptidomics analysis of PE placentas and performed bio-informatics
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IGF1R stimulates autophagy, enhances viability, and promotes insulin secretion in pancreatic β cells in gestational diabetes mellitus by upregulating ATG7 Reprod. Biol. (IF 2.1) Pub Date : 2024-01-22 Qing Liu, Ying Han, Meng Zhang, Peng Yang, Yan Xiang, Min Chen, Fei Xu, Xiaochan Zhou, Dan Zheng, Juan Qin
Gestational diabetes mellitus (GDM) is a prevalent metabolic disturbance in pregnancy. This article investigated the correlations between serum IGF1R and ATG7 with insulin resistance (IR) in GDM patients. Firstly, 100 GDM patients and 100 healthy pregnant women were selected as study subjects. The levels of serum IGF1, IGF1R, and ATG7 and their correlations with the insulin resistance index homeostasis
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Effect of pyometra on vascularity alterations, oxidative stress, histopathology and inflammatory molecules in feline Reprod. Biol. (IF 2.1) Pub Date : 2024-01-22 Elshymaa A. Abdelnaby, Abdulrhman K. Alhaider, Ibrahim M. Ghoneim, Noha Y. Salem, Eman S. Ramadan, Haithem A. Farghali, Marwa S. Khattab, Naglaa A. AbdElKader, Ibrahim A. Emam
This study aimed to investigate blood flow, hemodynamical features by Doppler ultrasound, the oxidative stress biomarkers from serum samples, and histopathology from uterine tissue, in healthy queens and queens with pyometra. Twenty queens were categorized into two groups, according to signs, history, and ultrasound findings, as pyometra and control healthy queens. Doppler ultrasonography, total antioxidant
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Exploring the potential use of platelet rich plasma (PRP) from adult and umbilical cord blood in murine follicle culture Reprod. Biol. (IF 2.1) Pub Date : 2024-01-17 Cristina Subiran Adrados, Jesús Cadenas, Sofie Lund Polat, Anna Sanderhage Tjäder, Paul Blanche, Stine Gry Kristensen
Ovarian follicle culture is a powerful tool to study follicular physiology and has potential applications in clinical and commercial settings. Despite remarkable progress, recreating folliculogenesis in vitro remains challenging for many mammalian species. This study investigates the impact of platelet-rich plasma (PRP) derived from adult blood (human platelet lysate, hPL) and umbilical cord blood
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Evaluation of the expression of growth hormone and its receptor during the resumption of postpartum ovarian follicle development in dairy cows Reprod. Biol. (IF 2.1) Pub Date : 2024-01-09 L.I. Durante, E. Angeli, L. Etchevers, U.S. Notaro, F.M. Rodríguez, H.H. Ortega, B.E. Marelli
Growth hormone is a key endocrine factor for metabolic adaptations to lactation and optimal reproductive function of the dairy cow. This study aimed to analyze the expression of GH and its receptor (GHR) in ovarian follicles, along with metabolic biomarkers, during the resumption of the postpartum follicular development, and to analyze the immunolocalization and protein expression of GH and GHR in
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Circulating levels of cytokines (IL-6, IL-10 and TGF- β) and CD4+CD25+FOXP3+Treg cell population in recurrent pregnancy loss Reprod. Biol. (IF 2.1) Pub Date : 2024-01-03 Sufaya Jameel, Rashmi Bhuwalka, Mahmooda Begum, Rajeshwari Bonu, Parveen Jahan
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Three-dimensional morphology of bovine blastocysts hatched against lipopolysaccharide exposure in vitro Reprod. Biol. (IF 2.1) Pub Date : 2023-12-30 Yasumitsu Masuda, Ryo Hasebe, Yasushi Kuromi, Minami Matsuo, Mitsugu Hishinuma, Tetsuya Ohbayashi, Ryo Nishimura
Embryo transfer in cattle is globally becoming more ubiquitous, but the pregnancy rate is lower than that of artificial insemination. The uterus contains its own bacteria, and concentrations of lipopolysaccharides (LPS) from gram-negative bacteria are higher in uteri affected by endometritis than in healthy uteri and they suppress embryogenesis. The purpose of this study was to investigate the morphological
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PFOS exposure destroys the integrity of the blood-testis barrier (BTB) through PI3K/AKT/mTOR-mediated autophagy Reprod. Biol. (IF 2.1) Pub Date : 2023-12-30 Zifeng Chen, Zhengru Chen, Sheng Gao, Jie Shi, Xinyao Li, Fei Sun
Perfluorooctanesulfonate or perfluorooctane sulfonic acid (PFOS), a type of perfluorinated compound, is mainly found in consumer products. Exposure to PFOS could cause male reproductive toxicity by causing injury to the blood-testis barrier (BTB). However, the specific mechanisms through which PFOS affects male reproduction remain unclear. The mammalian target of rapamycin (mTOR) is a vital protein
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Plasminogen activation and plasmin inhibition during in vitro fertilization in bovine: implications for fertilization parameters and early embryo development Reprod. Biol. (IF 2.1) Pub Date : 2023-12-30 Gabriela Rizo, Antonio Daniel Barrera, Elina Vanesa García, Mariela Roldán-Olarte
Components of the plasminogen/plasmin system, known to be present in the oocyte, play a key role in maturation and fertilization. The objective of this study was to examine the effect of plasminogen activation and plasmin inhibition by exogenous supplementation of the IVF medium with streptokinase (SK) or ɛ-aminocaproic acid (ε-ACA), respectively, on fertilization parameters and preimplantation embryo
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Serum exosomes from hepatitis B virus–infected patients inhibit glycolysis in Sertoli cells via miR-122-5p/ALDOA axis Reprod. Biol. (IF 2.1) Pub Date : 2023-12-30 Hao Deng, Yucheng Zhong, Jun Zhao, Xiaohang Li, Guoqun Luo, Huan Li
Hepatitis B virus (HBV) infection is associated with male infertility. The mechanism includes an increase in chromosomal instability in sperm, which has an adverse effect on sperm viability and function. Sertoli cells (SCs) are vital in spermatogenesis because they use glycolysis to provide energy to germ cells and themselves. HBV infection impairs sperm function. However, whether HBV infection disrupts
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Knockdown of TXNIP alleviates gestational diabetes mellitus by activating autophagy to regulate cell proliferation and apoptosis in high glucose-treated trophoblasts Reprod. Biol. (IF 2.1) Pub Date : 2023-12-19 Ying Gu, Minkai Cao, Yu Chen, Jingyang Li, Lingli Hu, Xiao-Jun Yang
Dysregulated thioredoxin-interacting protein (TXNIP) has been observed in women with gestational diabetes mellitus (GDM), but the specific role of TXNIP in GDM and the underlying mechanism remain unclear. HTR-8/SVneo cells were treated with high glucose to mimic the injured trophoblasts of GDM. In vitro, TXNIP knockdown was performed by siRNA. RTqPCR was performed to determine the expression of the
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Decreased fertility in female mice lacking urokinase plasminogen activator Reprod. Biol. (IF 2.1) Pub Date : 2023-12-19 Gaia Laurenzi, Valeria Fedeli, Rita Canipari
It is well established that mouse ovarian granulosa cells secrete urokinase plasminogen activator (uPA) under gonadotropin stimulation. The synthesis and secretion of the enzyme correlate well with the time of follicular rupture in vivo. Moreover, uPA is secreted by the trophoblast at the time of implantation. In the present study, we have analyzed whether the absence of uPA could influence follicular
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Morphohistometric and steroidogenic parameters during testicular and epididymal differentiation in cavy (Galea spixii) fetuses Reprod. Biol. (IF 2.1) Pub Date : 2023-11-30 Júlia Moreira Silveira, Amilton Cesar dos Santos, Danielle Cristina Calado de Brito, Moacir Franco de Oliveira, Alan James Conley, Antonio Chaves de Assis Neto
Sexual differentiation and steroidogenic mechanisms have an important impact on postnatal gonadal phenotypic development. Thus, establishing the activities that lead to male phenotypic development can provide a better understanding of this process. This study examined the prenatal development of cavies to establish morphological and histometric development patterns and protein and enzyme immunolocalization
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Expression and in vitro effect of phoenixin-14 on the porcine ovarian granulosa cells Reprod. Biol. (IF 2.1) Pub Date : 2023-11-27 Patrycja Kurowska, Ewa Mlyczyńska, Julia Wajda, Konrad Król, Karolina Pich, Patrycja Guzman, Aleksandra Greggio, Oliwia Szkraba, Małgorzata Opydo, Joelle Dupont, Agnieszka Rak
Phoenixin-14 (PNX-14) regulates energy metabolism via the G protein-coupled receptor 173 (GPR173); elevated plasma levels have been described in patients with polycystic ovary syndrome. The aims were to investigate the ovarian expression of PNX-14/GPR173 and the in vitro effect of PNX-14 on granulosa cells (Gc) function. Transcript and protein levels of PNX-14/GRP173 were analysed by real-time PCR
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Neurotensin agonist PD149163 modulates lipopolysaccharide induced inflammation and oxidative stress in the female reproductive system of mice Reprod. Biol. (IF 2.1) Pub Date : 2023-11-29 Sonia Srivastava, Swarnima Mishra, Gyan Babu, Banalata Mohanty
Inflammation-mediated reproductive health problems in females have become an emerging concern. The present investigation was aimed to elucidate the efficacy of the PD149163, agonist of the type I neurotensin receptor, in preventing/ameliorating the lipopolysaccharide (LPS) induced inflammation of the female reproductive system of the mice. Female Swiss Albino Mice (8 weeks old) were maintained in three
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Serum miRNA as a predictive biomarker for ovarian reserve after endometrioma-cystectomy Reprod. Biol. (IF 2.1) Pub Date : 2023-11-22 Atsushi Yabuki, Ayako Muraoka, Satoko Osuka, Akira Yokoi, Kosuke Yoshida, Masami Kitagawa, , Reina Sonehara, Natsuki Miyake, Natsuki Nakanishi, Tomoko Nakamura, Akira Iwase, Hiroaki Kajiyama
Ovarian endometrioma (OE) is a common gynecological disease that is often treated with surgery and hormonal treatment. However, ovarian cystectomy can impair the ovarian reserve (OR). Previously, we showed that perioperative administration of dienogest (DNG) is an effective option for OR preservation. However, there were differences in the extent of OR preservation among patients following perioperative
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ECM1 promotes migration and invasion in endometriosis Reprod. Biol. (IF 2.1) Pub Date : 2023-11-22 Chen Zhang, Hongyan Cheng, Xue Ye, Heng Cui, Yi Li, Honglan Zhu, Xiaohong Chang
Extracellular matrix protein 1 (ECM1) is a glycoprotein that may be a key player in tumorigenesis and tumor progression. However, knowledge regarding the role of ECM1 in endometriosis (EM) is still lacking. Microarray analyses were performed to compare the mRNA expression patterns between paired EU tissues and ectopic endometrial (EC) tissues (n = 4) from EM patients. ECM1 expression was significantly
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ICSI outcome after microdissection testicular sperm extraction, testicular sperm aspiration and ejaculated sperm Reprod. Biol. (IF 2.1) Pub Date : 2023-11-24 Rauni Klami, Candido Tomás, Harri Mankonen, Antti Perheentupa
We conducted a case-controlled single-center cohort study to evaluate the intracytoplasmic sperm injection (ICSI) outcome in severe male infertility with different methods of sperm obtention. The data was compiled from a tertiary university hospital. The micro-TESE procedures were performed from 2008 to 2023, with a sperm recovery rate (SRR) of 45 %. The ICSI treatments were carried out between 2011
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Regulatory mechanisms of SoxD transcription factors and their influences on male fertility Reprod. Biol. (IF 2.1) Pub Date : 2023-11-17 Mariama Diawara, Luc J. Martin
Members of the SRY-related box (SOX) subfamily D (SoxD) of transcription factors are well conserved among vertebrate species and play important roles in different stages of male reproductive development. In mammals, the SoxD subfamily contains three members: SOX5, SOX6 and SOX13. Here, we describe their implications in testicular development and spermatogenesis, contributing to fertility. We also cover
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Expression of angiogenic factors in the placenta of heme oxygenase-1 deficient mouse embryo Reprod. Biol. (IF 2.1) Pub Date : 2023-11-17 Meenakshi Rana, Pooja Choubey, Gouri Nandi, Sidhant Jain, Divya Bajaj, Sonika Sharma, Sharmila Basu-Modak
Heme oxygenase 1 (Hmox1), the inducible form of heme degrading enzymes Hmoxs, is important for establishment and maintenance of pregnancy. A growing body of evidence suggests an association between Hmox1 and angiogenesis, including placental angiogenesis. In this study, we examined the expression of two angiogenic factors in the placentas of Hmox1 deficient mouse embryos, whose expression was found
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Evaluation of the therapeutic effects of arbutin on cisplatin‐induced ovarian toxicity in rats through endoplasmic reticulum stress and Nrf2 pathway Reprod. Biol. (IF 2.1) Pub Date : 2023-11-16 Elif Ayazoglu Demir, Ahmet Mentese, Zeynep Sagnak Yilmaz, Nihal Turkmen Alemdar, Selim Demir, Yuksel Aliyazicioglu
Arbutin (ARB) is a glycosylated hydroquinone with potent antioxidant effects. Although cisplatin (CP) is widely used in chemotherapy, its toxicity in healthy tissues, including ovotoxicity, is an insurmountable problem. This study aimed to evaluate the therapeutic effect of ARB against CP-related ovototoxicity by including nuclear factor erythroid 2-related factor 2 (Nrf2) pathway in rats for the first
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Detection and quantification of TIMP1 and miR-141 through RT-LAMP and TT-LAMP in serum samples during estrous cycle in buffalo Reprod. Biol. (IF 2.1) Pub Date : 2023-11-04 Mansi Joshi, Rajeev Chandel, Varun Kumar, Sanjay Sharma, Rubina Kumari Baithalu, Dheer Singh, Suneel Kumar Onteru
Estrus identification is a common problem in the reproductive management of farm animals. Hence, several studies have been conducted to explore biomarkers for estrus detection. One of our previous studies identified the abundance of RNA biomarkers such as TIMP1 and miR-141 in buffalo saliva during the estrus stage. However, the level of these RNA biomarkers in buffalo serum during estrous cycle is
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Toxicological effects of the Curatella americana extract in embryo development of female pups from diabetic rats Reprod. Biol. (IF 2.1) Pub Date : 2023-10-31 Larissa Lopes Cruz, Vinícius Soares Barco, Verônyca Gonçalves Paula, Maysa Rocha Souza, Franciane Quintanilha Gallego, Gean Charles Monteiro, Giuseppina Pace Pereira Lima, Débora Cristina Damasceno, Gustavo Tadeu Volpato
Maternal diabetes can influence the development of offspring during fetal life and postnatally. Curatella americana is a plant used as a menstrual cycle regulator and to prevent diabetes. This study evaluates the effects of C. americana aqueous extract on the estrous cycle and preimplantation embryos of adult female pups from diabetic rats. Female Sprague Dawley newborn rats received Streptozotocin
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Ferroportin expression and regulation in human placenta/fetal membranes: Implications for ferroptosis and adverse pregnancy outcomes Reprod. Biol. (IF 2.1) Pub Date : 2023-10-25 Shu-Wing Ng, Chungyan Lee, Allen Ng, Shu-Kay Ng, Felice Arcuri, Michael D. House, Errol R. Norwitz
Iron overload is associated with pregnancy complications. Ferroportin (FPN) is the only known iron exporter in mammalian cells. We hypothesize that FPN is functionally important in ferrotopsis, a process of iron-dependent non-apoptotic programmed cell death, and may have a critical role to play in pregnancy success. We investigated the expression of FPN in placenta/fetal membranes by immunohistochemistry
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Low dose rate radiation impairs early follicles in young mice Reprod. Biol. (IF 2.1) Pub Date : 2023-10-25 Se Yoon Seong, Min Kook Kang, Hyunju Kang, Hae-June Lee, Yeong-Rok Kang, Chang Geun Lee, Dong Hyun Sohn, Seung Jin Han
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Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue Reprod. Biol. (IF 2.1) Pub Date : 2023-10-25 Morgana A. Borges, Bruna R. Curcio, Gustavo D.A. Gastal, Luiza Gheno, Antonio S. Varela Junior, Carine D. Corcini, Carlos E.W. Nogueira, Francisco L.N. Aguiar, Eduardo L. Gastal
The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy’s (CAR) solution and paraformaldehyde
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Biomolecular composition of porcine ovarian follicles following in vitro treatment of vitamin D3 and insulin alone or in combination Reprod. Biol. (IF 2.1) Pub Date : 2023-10-18 Kinga Kamińska, Ewelina Wiercigroch, Kamilla Małek, Małgorzata Grzesiak
The study aimed to analyze changes in biomolecular composition of granulosa and theca interna cells of porcine ovarian follicles following in vitro treatment of vitamin D3 and insulin alone or in combination. Medium antral follicles (n = 4/each group) were cultured alone (C; control) or in the presence of 1α,25(OH)2D3 (VD; 100 ng/mL) and insulin (I; 10 ng/mL) separately or in combination, VD and I
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High-fructose consumption suppresses insulin signaling pathway accompanied by activation of macrophage and apoptotic markers in rat testis Reprod. Biol. (IF 2.1) Pub Date : 2023-10-14 Onur Gökhan Yildirim, Ceren Guney, Mehmet Eray Alcigir, Fatma Akar
Dietary high-fructose may cause metabolic disturbances; however, its effect on the reproductive system is little understood. The insulin signaling pathway is critical in testicular development, maintenance of microcirculation and spermatogenesis. Therefore, in this study, we aimed to investigate the impact of dietary high-fructose on insulin signaling pathway as well as macrophage and apoptotic markers
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Cystic endometrial hyperplasia-pyometra syndrome impairs the preantral follicle reserve in domestic bitches (Canis familiaris) Reprod. Biol. (IF 2.1) Pub Date : 2023-10-11 Mylene G.M. Camozzi, Klaus C. Saturnino, Mônica R.F. Machado, Gustavo D.A. Gastal, Cecília N. Moreira, Benner G. Alves
Cystic endometrial hyperplasia (CEH)-pyometra syndrome is the most common uterine pathological condition reported in breeding bitches, however, their described effects on fertility are limited to uterine disorders and conception rates. As the preantral follicle population represents the available reserve of gametes recruited during the lifespan, the aim of this study was to evaluate the effects of
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Oocyte quality is impaired in a hyperandrogenic PCOS mouse model by increased Foxo1 expression Reprod. Biol. (IF 2.1) Pub Date : 2023-10-06 Du Danfeng, Deng Ke, Fan Dengxuan, Li Xuelian, Xu Congjian
One of the most important characteristics of patients with polycystic ovary syndrome (PCOS) is excess androgen, which has adverse effects on pregnancy outcomes and increases the risk of offspring developing metabolic disorders. Foxo1 has been shown to play an important role in PCOS, but whether it has an affect on oocyte’s quality in PCOS remains unclear. The current research investigated the effect
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The effects of pretreatment with Cyclosporin A and Docetaxel before vitrification of porcine immature oocytes on subsequent embryo development Reprod. Biol. (IF 2.1) Pub Date : 2023-09-15 Hong Thi Nguyen, Nhung Thi Nguyen, Linh Viet Nguyen, Xuan Nguyen Bui, Van Hanh Nguyen, Van Khanh Nguyen, Huong Thu Thi Vu, Sam Thi Nguyen, Hiep Thi Nguyen
In the present study, we attempted to improve the developmental competence of vitrified immature porcine oocytes by the preservation of mitochondrial properties using Cyclosporin A (CsA, inhibitor of mitochondrial membrane permeability transition) and Docetaxel (stabilizer of microtubules, hence mitochondrial distribution). In Experiment 1, Mitotracker red staining revealed reduced mitochondrial activity
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LC-MS-based untargeted metabolomics reveals the mechanism underlying prostate damage in a type 2 diabetes mouse model Reprod. Biol. (IF 2.1) Pub Date : 2023-09-01 Rong Qi, Shao-san Kang, Yongchao Pei, Mingming Liu, Yufan Zhou, Bo Guan, Xinduo Zhang, Zhiguo Li, Fenghong Cao
Type 2 diabetes mellitus (T2DM) can cause prostate damage and affect male reproductive function, but the underlying mechanisms are not completely understood. In this study, we used liquid chromatography-mass spectrometry (LC-MS)-based untargeted metabolomics to identify endogenous metabolites in the prostate of a T2DM mouse model. The selected endogenous metabolites were then subjected to bioinformatics
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Mechanisms of DHEA-induced AMH increase in the ovarian tissues of PCOS rat Reprod. Biol. (IF 2.1) Pub Date : 2023-08-24 Yiwen Zhang, Xiaomeng Yu, lili liu, Jindan Zheng
The present study aimed to build a DHEA-induced polycystic ovary syndrome (PCOS) rat model to evaluate the potential mechanism of DHEA-induced AMH rise in these rat ovarian tissues. A total of 36 female 3-week-old rats were allocated into two groups at random. The control group received merely the same amount of sesame oil for 20 days while the experimental group received 0.2 mL of sesame oil Plus
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Circulating miR-3613-5p but not miR-125b-5p, miR-199a-3p, and miR-451a are biomarkers of endometriosis Reprod. Biol. (IF 2.1) Pub Date : 2023-08-21 Izabela Walasik, Klaudia Klicka, Tomasz M. Grzywa, Iwona Szymusik, Paweł Włodarski, Mirosław Wielgoś, Bronisława Pietrzak, Artur Ludwin
Objective This study aimed to assess the utility of circulating miR-125b-5p, miR-199a-3p, miR-451a, and miR-3613-5p as biomarkers of endometriosis. Study design Patients with stage III or IV of endometriosis according to the revised American Society of Reproductive Medicine (rASRM) staging classification, as well as control women, were recruited. We created a prospective study conducted on a group
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Manipulated sperm motility via soot nanoparticles-induced biochemical alterations in human seminal plasma Reprod. Biol. (IF 2.1) Pub Date : 2023-08-18 Karekin D. Esmeryan, Ivaylo Rangelov, Todor A. Chaushev
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Ghrelin and obestatin can promote human ovarian granulosa cell functions and FSH effects Reprod. Biol. (IF 2.1) Pub Date : 2023-08-14 Alexander V. Sirotkin, Adam Tarko, Miloš Mlynček, Abdel Halim Harrath
The aim of the present in-vitro experiments was to examine the direct influence of ghrelin and obestatin on viability, proliferation and progesterone release by human ovarian granulosa cells and their response to FSH administration. Human granulosa cells were cultured in presence of ghrelin or obestatin (both at 0, 1, 10 or 100 ng/ml) alone or in the presence of FSH (10 ng/ml). Cell viability, accumulation
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Promising effects of exosomes from menstrual blood-derived mesenchymal stem cells on endometriosis Reprod. Biol. (IF 2.1) Pub Date : 2023-08-04 Faezeh Davoodi Asl, Seyedeh Saeideh Sahraei, Naser Kalhor, Hoda Fazaeli, Mohsen Sheykhhasan, Sanaz Soleimani Moud, Leila Naserpour, Azar Sheikholeslami
Endometriosis as a non-malignant gynecological disease leads to dysregulation of numerous cellular functions including apoptosis, angiogenesis, migration, proliferation, and inflammation. Accumulating evidence has shed light on the importance of endometrial stem cells within the menstrual blood which are involved in the establishment and progression of endometriotic lesions in a retrograde manner.
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Impact of prepubertal bovine ovarian tissue pre-freeze holding duration on follicle quality Reprod. Biol. (IF 2.1) Pub Date : 2023-07-31 Divya Deeleep Bhagat Amonkar, Vincenzo Genovese, Vincenza De Gregorio, Angela Travaglione, Shubhashree Uppangala, R. Vani Lakshmi, Guruprasad Kalthur, Roberto Gualtieri, Riccardo Talevi, Satish Kumar Adiga
Ovarian tissue cryopreservation prior to gonadotoxic treatment is the only recommended option for fertility preservation in prepubertal girls. Due to the technical complexity of this technique, limited number of centres across the world are equipped to offer the facility. Hence, the retrieved ovarian tissue needs to be maintained at hypothermic temperature (4 °C) for long time during shipment. The
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Protective effect of exogenous melatonin on testicular histopathology and histomorphometry of adult rats with domperidone-induced hyperprolactinemia Reprod. Biol. (IF 2.1) Pub Date : 2023-07-28 Ewerton Fylipe de Araújo Silva, José Anderson da Silva Gomes, Maria Luísa Figueira de Oliveira, Arthur Gabriel Alves Furtado de Carvalho Noya, Carolina Peixoto Magalhães, João Vitor da Silva, Luiz Henrique da Silva, Bruno Mendes Tenorio, Marcelo Moraes Valença, Fernanda das Chagas Angelo Mendes Tenorio
Hyperprolactinemia is a pathological condition resulting from increased prolactin that directly affects reproduction, as this condition inhibits the release of LH, FSH and gonadal steroidogenesis, bringing several negative clinical associations in reproduction. In contrast, melatonin (MEL) plays an important role in the regulation of steroidogenesis and modulates damages to the process of spermatogenesis
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APE1 promotes embryonic stem cell proliferation and teratoma formation by regulating GDNF/GFRα1 axis Reprod. Biol. (IF 2.1) Pub Date : 2023-07-29 Ling Liu, Qiang Wu, Zan Wang, Bobo Niu, Yaguang Jiao, Huibo An
The teratomas formation has severely hindered the application of embryonic stem cells (ESCs) in clinical trials. Apurinic/apyrimidinic endonuclease 1 (APE1) is strongly involved in the development of tumors and differentiation process of stem cells. However, the role of APE1 in teratomas remains unknown. The expression of APE1 was examined in mouse ESCs (mESCs) by reverse transcription quantitative
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Cell apoptosis in the testis of male rats is elevated by intervention with β-endorphin and the mu opioid receptor Reprod. Biol. (IF 2.1) Pub Date : 2023-07-25 Xiaofan Xiong, Siyu Zhang, Lulu Zang, Yanlong Xin, Yixin Pang, Shuting Zhang, Yu Yang, Rongfu Tu, Lingyu Zhang, Yuefeng Du, Juan Yang
β-endorphin (β-EP) is involved in the regulation of male germ cells; however, little is known about the effect of β-EP on primary germ cells via opioid receptors. In this study, we first revealed significant cell apoptosis in the testis of male rats after β-EP intervention. Subsequently, the expression of the mu opioid receptor (MOR) was detected in both Leydig cells (LCs) and spermatogonia (SGs) by
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Orotic acid induces apoptotic death in ovarian adult granulosa tumour cells and increases mitochondrial activity in normal ovarian granulosa cells Reprod. Biol. (IF 2.1) Pub Date : 2023-07-19
Orotic acid (OA) is a natural product that acts as a precursor in the pyrimidine nucleotide biosynthesis pathway. Most studies concerning administration of OA focus on its therapeutic effects; however, its effect on tumours is unclear. We aimed to determine whether treatment with OA influences the viability and apoptosis of normal (HGrC1) and tumour-derived (KGN) human ovarian granulosa cells. The
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The relationship between ultrasonographically measured testicular volumes and cord blood inhibin B concentrations in healthy term male neonates Reprod. Biol. (IF 2.1) Pub Date : 2023-07-08 Betül Ersoy, Gülgün Yılmaz Ovalı, Ebru Canda, Ece Onur, Beyhan Cengiz Özyurt, Nermin Tansuğ
Serum inhibin B (INHB) concentrations are associated with testicular volumes (TV) in all periods of childhood. The aim of the study was to investigate the relationship between TV measured by ultrasonography (US) and cord blood inhibin B and total testosterone (TT) concentrations, stratified by mode of delivery. In total 90 male infants were included. Testes of healthy, term newborns were evaluated
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CXCR4, regulated by HIF1A, promotes endometrial breakdown via CD45+ leukocyte recruitment in a mouse model of menstruation Reprod. Biol. (IF 2.1) Pub Date : 2023-06-29 Shufang Wang, Xihua Chen, Shige Guo, Fang Zhou, Xin Zhang, Cong Lu, Xuqing Yang, Qianxing Wang, Bin He, Jiedong Wang, Hanbi Wang, Xiangbo Xu
Menstruation is a specific physiological phenomenon in female humans that is regulated by complex molecular mechanisms. However, the molecular network involved in menstruation remains incompletely understood. Previous studies have suggested that C-X-C chemokine receptor 4 (CXCR4) is involved; however, how CXCR4 participates in endometrial breakdown remains unclear, as do its regulatory mechanisms.
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The long noncoding RNA CIRBIL is a regulator of steroidogenesis in mice Reprod. Biol. (IF 2.1) Pub Date : 2023-06-17 Xiang Huang, Xin Liu, Xiaofang Zhang, Ying Yang, Haiyu Gao, Jianjun Gao, Hairong Bao, Lexin Zhao, Guohui Yang, Yang Zhang, Donghua Liu
Infertility affects roughly 8–12 % of couples worldwide, and in above 50 % of couples, male factors are the primary or contributing cause. Many long noncoding RNAs (lncRNAs) are detected in the testis, but their functions are not well understood. CIRBIL was 862 nucleotides in length and was found to be localized mostly in the cytosol of Leydig cell, a small portion was positioned inside the seminiferous
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Disturbed ovarian morphology, oestrous cycling and fertility of high fat fed rats are linked to alterations of incretin receptor expression Reprod. Biol. (IF 2.1) Pub Date : 2023-06-19 Dawood Khan, Ananyaa Sridhar, Peter R. Flatt, R.Charlotte Moffett
Obesity is a major cause of infertility in females with a direct correlation between energy intake and reproductive dysfunction. To explore underlying mechanisms, disturbances in reproductive health and incretin/reproductive hormone receptor expression were studied in female Wistar rats fed a high-fat-diet for 20-weeks. Metabolic parameters and ovarian/adrenal gene expression were monitored along with
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LncRNA PWRN2 promotes polycystic ovary syndrome progression via epigenetically reducing ATRX by recruiting LSD1 Reprod. Biol. (IF 2.1) Pub Date : 2023-06-13 Zhaoqing Gong, Xinlei Shi, Weizhen Xu, Yuan Fang, Meijia Fang, Minhua Yao, Yu Jiang, Hongshu Sui, Mingjiu Luo
Long non-coding RNA has been shown to mediate the progression of polycystic ovary syndrome (PCOS). However, the role and mechanism of Prader-Willi region nonprotein coding RNA 2 (PWRN2) in PCOS progression remain unclear. In our study, Sprague-Dawley rat was injected with dehydroepiandrosterone to mimic PCOS rat models. HE staining was used to assess the number of benign granular cells, and serum insulin
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Gastrodin protects porcine sertoli cells from zearalenone-induced abnormal secretion of glial cell line-derived neurotrophic factor through the NOTCH signaling pathway Reprod. Biol. (IF 2.1) Pub Date : 2023-06-06 Yulu Cao, Jingjing Wang, Xiaoxiao Li, Biyun Liu, Chongjun Li, Yijin Sun, Kang Zou
Zearalenone (ZEA) is a prevalent mycotoxin found in moldy diets and is associated with reproductive dysfunction. However, the molecular underpinning of ZEA in impairment of spermatogenesis remains largely unknown. To unveil the toxic mechanism of ZEA, we established a co-culture model using porcine Sertoli cells and porcine spermatogonial stem cells (pSSCs) to investigate the impact of ZEA on these
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Application of Matrigel in the 3-dimension culture of female germline stem cells Reprod. Biol. (IF 2.1) Pub Date : 2023-05-22 Wen Zhang, Yang Cheng, Shu Zhang, Rui Wei, Kang Zou
Female germline stem cells (FGSCs) are a group of rare undifferentiated cells found in ovarian cortex, which have the unique ability to self-renew and differentiate. Stable maintenance and proliferation of FGSCs in culture are pivotal for clinic research. However, conventional 2-D (dimension) culture systems are limited in their ability to mimic the ovarian microenvironment during in vitro studies