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Multimode fiber endoscopes for computational brain imaging Neurophotonics (IF 5.3) Pub Date : 2024-03-01 Lyubov V. Amitonova
Advances in imaging tools have always been a pivotal driver for new discoveries in neuroscience. An ability to visualize neurons and subcellular structures deep within the brain of a freely behaving animal is integral to our understanding of the relationship between neural activity and higher cognitive functions. However, fast high-resolution imaging is limited to sub-surface brain regions and generally
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Photonic neural probe enabled microendoscopes for light-sheet light-field computational fluorescence brain imaging Neurophotonics (IF 5.3) Pub Date : 2024-02-01 Peisheng Ding, Hannes Wahn, Fu-Der Chen, Jianfeng Li, Xin Mu, Andrei Stalmashonak, Xianshu Luo, Guo-Qiang Lo, Joyce K. S. Poon, Wesley D. Sacher
SignificanceLight-sheet fluorescence microscopy is widely used for high-speed, high-contrast, volumetric imaging. Application of this technique to in vivo brain imaging in non-transparent organisms has been limited by the geometric constraints of conventional light-sheet microscopes, which require orthogonal fluorescence excitation and collection objectives. We have recently demonstrated implantable
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Fiber-based in vivo imaging: unveiling avenues for exploring mechanisms of synaptic plasticity and neuronal adaptations underlying behavior Neurophotonics (IF 5.3) Pub Date : 2024-02-01 Anna Karpova, Ahmed A. A. Aly, Endre Levente Marosi, Sanja Mikulovic
In recent decades, various subfields within neuroscience, spanning molecular, cellular, and systemic dimensions, have significantly advanced our understanding of the elaborate molecular and cellular mechanisms that underpin learning, memory, and adaptive behaviors. There have been notable advancements in imaging techniques, particularly in reaching superficial brain structures. This progress has led
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Recent advances in light patterned optogenetic photostimulation in freely moving mice Neurophotonics (IF 5.3) Pub Date : 2024-02-01 Antonio Lorca-Cámara, François G. C. Blot, Nicolò Accanto
Optogenetics opened the door to a new era of neuroscience. New optical developments are under way to enable high-resolution neuronal activity imaging and selective photostimulation of neuronal ensembles in freely moving animals. These advancements could allow researchers to interrogate, with cellular precision, functionally relevant neuronal circuits in the framework of naturalistic brain activity
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Advancing the path to in-vivo imaging in freely moving mice via multimode-multicore fiber based holographic endoscopy Neurophotonics (IF 5.3) Pub Date : 2024-02-01 Yang Du, Evelyn Dylda, Miroslav Stibůrek, André D Gomes, Sergey Turtaev, Janelle M. P. Pakan, Tomáš Čižmár
SignificanceHair-thin multimode optical fiber-based holographic endoscopes have gained considerable interest in modern neuroscience for their ability to achieve cellular and even subcellular resolution during in-vivo deep brain imaging. However, the application of multimode fibers in freely moving animals presents a persistent challenge as it is difficult to maintain optimal imaging performance while
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Resolution-enhanced multi-core fiber imaging learned on a digital twin for cancer diagnosis Neurophotonics (IF 5.3) Pub Date : 2024-01-01 Tijue Wang, Jakob Dremel, Sven Richter, Witold Polanski, Ortrud Uckermann, Ilker Eyüpoglu, Jürgen W. Czarske, Robert Kuschmierz
SignificanceDeep learning enables label-free all-optical biopsies and automated tissue classification. Endoscopic systems provide intraoperative diagnostics to deep tissue and speed up treatment without harmful tissue removal. However, conventional multi-core fiber (MCF) endoscopes suffer from low resolution and artifacts, which hinder tumor diagnostics.AimWe introduce a method to enable unpixelated
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“There’s plenty of room at the bottom”: deep brain imaging with holographic endo-microscopy Neurophotonics (IF 5.3) Pub Date : 2024-01-01 Hana Uhlířová, Miroslav Stibůrek, Tomáš Pikálek, André Gomes, Sergey Turtaev, Petra Kolbábková, Tomáš Čižmár
SignificanceOver more than 300 years, microscopic imaging keeps providing fundamental insights into the mechanisms of living organisms. Seeing microscopic structures beyond the reach of free-space light-based microscopy, however, requires dissection of the tissue—an intervention seriously disturbing its physiological functions. The hunt for low-invasiveness tools has led a growing community of physicists
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Standardized 3D test object for multi-camera calibration during animal pose capture. Neurophotonics (IF 5.3) Pub Date : 2023-11-07 Hao Hu,Roark Zhang,Tony Fong,Helge Rhodin,Timothy H Murphy
Accurate capture of animal behavior and posture requires the use of multiple cameras to reconstruct three-dimensional (3D) representations. Typically, a paper ChArUco (or checker) board works well for correcting distortion and calibrating for 3D reconstruction in stereo vision. However, measuring the error in two-dimensional (2D) is also prone to bias related to the placement of the 2D board in 3D
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It takes two (seconds): decreasing encoding time for two-choice functional near-infrared spectroscopy brain-computer interface communication. Neurophotonics (IF 5.3) Pub Date : 2023-11-02 Anna Vorreuther,Lisa Bastian,Amaia Benitez Andonegui,Danielle Evenblij,Lars Riecke,Michael Lührs,Bettina Sorger
Significance Brain-computer interfaces (BCIs) can provide severely motor-impaired patients with a motor-independent communication channel. Functional near-infrared spectroscopy (fNIRS) constitutes a promising BCI-input modality given its high mobility, safety, user comfort, cost-efficiency, and relatively low motion sensitivity. Aim The present study aimed at developing an efficient and convenient
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Fiber photometry-based investigation of brain function and dysfunction Neurophotonics (IF 5.3) Pub Date : 2023-11-01 Nicole Byron, Shuzo Sakata
Fiber photometry is an optical method to monitor fluorescent signals using a fiber optic cannula. Over the past two decades, together with the development of various genetically encoded biosensors, it has been applied to investigate various types of activity in the central nervous system. This includes not only type-specific neuronal population activity, but also non-neuronal activity and neurotra
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Enhanced synaptic protein visualization by multicolor super-resolution expansion microscopy. Neurophotonics (IF 5.3) Pub Date : 2023-10-25 Janna Eilts,Sebastian Reinhard,Nikolas Michetschläger,Christian Werner,Markus Sauer
Significance Understanding the organization of biomolecules into complexes and their dynamics is crucial for comprehending cellular functions and dysfunctions, particularly in neuronal networks connected by synapses. In the last two decades, various powerful super-resolution (SR) microscopy techniques have been developed that produced stunning images of synapses and their molecular organization. However
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Chasing cortical behavior: designing multiphoton microscopes for imaging neuronal populations in freely moving rodents. Neurophotonics (IF 5.3) Pub Date : 2023-10-25 Alexandr Klioutchnikov,Jason N D Kerr
Imaging in the freely moving animal gives unparalleled access to circuit activity as the animal interacts with its environment in a self-guided way. Over the past few years, new imaging technologies have enabled the interrogation of neuronal populations located at any depth of the cortex in freely moving mice while preserving the animal's behavioral repertoire. This commentary gives an updated overview
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Low throughput screening in neuroscience: using light to study central synapses one at a time. Neurophotonics (IF 5.3) Pub Date : 2023-10-24 Léa Caya-Bissonnette,Jean-Claude Béïque
Neurophotonic approaches have fostered substantial progress in our understanding of the brain by providing an assortment of means to either monitor or manipulate neural processes. Among these approaches, the development of two-photon uncaging provides a useful and flexible approach to manipulate the activity of individual synapses. In this short piece, we explore how this technique has emerged at the
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Low-cost, smartphone-based instant three-dimensional registration system for infant functional near-infrared spectroscopy applications. Neurophotonics (IF 5.3) Pub Date : 2023-10-23 Yunjia Xia,Kui Wang,Addison Billing,Matthew Billing,Robert J Cooper,Hubin Zhao
Significance To effectively apply functional near-infrared spectroscopy (fNIRS)/diffuse optical tomography (DOT) devices, a three-dimensional (3D) model of the position of each optode on a subject's scalp and the positions of that subject's cranial landmarks are critical. Obtaining this information accurately in infants, who rarely stop moving, is an ongoing challenge. Aim We propose a smartphone-based
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Functional near-infrared spectroscopy-based prefrontal cortex oxygenation during working memory tasks in sickle cell disease. Neurophotonics (IF 5.3) Pub Date : 2023-10-17 John Sunwoo,Payal Shah,Wanwara Thuptimdang,Maha Khaleel,Patjanaporn Chalacheva,Roberta M Kato,Thomas D Coates,Michael C K Khoo
Significance Sickle cell disease (SCD), characterized by painful vaso-occlusive crises, is associated with cognitive decline. However, objective quantification of cognitive decline in SCD remains a challenge, and the associated hemodynamics are unknown. Aim To address this, we utilized functional near-infrared spectroscopy (fNIRS) to measure prefrontal cortex (PFC) oxygenation responses to N-back working
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Cerebrovascular reactivity to carbon dioxide tension in newborns: data from combined time-resolved near-infrared spectroscopy and diffuse correlation spectroscopy. Neurophotonics (IF 5.3) Pub Date : 2023-10-14 Sofia Passera,Agnese De Carli,Monica Fumagalli,Davide Contini,Nicola Pesenti,Caterina Amendola,Martina Giovannella,Turgut Durduran,Udo M Weigel,Lorenzo Spinelli,Alessandro Torricelli,Gorm Greisen
Significance Critically ill newborns are at risk of brain damage from cerebrovascular disturbances. A cerebral hemodynamic monitoring system would have the potential role to guide targeted intervention. Aim To obtain, in a population of newborn infants, simultaneous near-infrared spectroscopy (NIRS)-based estimates of cerebral tissue oxygen saturation (StO2) and blood flow during variations of carbon
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Acute hypoxia elicits prefrontal oxygenation asymmetry in young adults. Neurophotonics (IF 5.3) Pub Date : 2023-10-05 Kojiro Ide
Significance Cerebrovascular reactivity can be evaluated by prefrontal cortex (PFC) hemodynamic responses and oxygenation changes secondary to hypoxia using near-infrared spectroscopy (NIRS). However, whether there are hemispheric differences in these NIRS-determined PFC hemodynamic responses and oxygenation changes remains unknown. Aim This study was performed to determine whether there are differences
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Characterizing the blood-brain barrier and gut barrier with super-resolution imaging: opportunities and challenges. Neurophotonics (IF 5.3) Pub Date : 2023-10-04 Ellen Doney,Renaud Bernatchez,Valérie Clavet-Fournier,Katarzyna A Dudek,Laurence Dion-Albert,Flavie Lavoie-Cardinal,Caroline Menard
Brain and gut barriers have been receiving increasing attention in health and diseases including in psychiatry. Recent studies have highlighted changes in the blood-brain barrier and gut barrier structural properties, notably a loss of tight junctions, leading to hyperpermeability, passage of inflammatory mediators, stress vulnerability, and the development of depressive behaviors. To decipher the
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Quantitative hemodynamic imaging: a method to correct the effects of optical properties on laser speckle imaging. Neurophotonics (IF 5.3) Pub Date : 2023-10-03 Thinh Phan,Christian Crouzet,Gordon T Kennedy,Anthony J Durkin,Bernard Choi
Significance Studying cerebral hemodynamics may provide diagnostic information on neurological conditions. Wide-field imaging techniques, such as laser speckle imaging (LSI) and optical intrinsic signal imaging, are commonly used to study cerebral hemodynamics. However, they often do not account appropriately for the optical properties of the brain that can vary among subjects and even during a single
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Zebrafish brain atlases: a collective effort for a tiny vertebrate brain. Neurophotonics (IF 5.3) Pub Date : 2023-09-30 Antoine Légaré,Mado Lemieux,Patrick Desrosiers,Paul De Koninck
In the past two decades, digital brain atlases have emerged as essential tools for sharing and integrating complex neuroscience datasets. Concurrently, the larval zebrafish has become a prominent vertebrate model offering a strategic compromise for brain size, complexity, transparency, optogenetic access, and behavior. We provide a brief overview of digital atlases recently developed for the larval
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Shining light on the noradrenergic system. Neurophotonics (IF 5.3) Pub Date : 2023-09-26 Emmeraude Tanguay,Sarah-Julie Bouchard,Martin Lévesque,Paul De Koninck,Vincent Breton-Provencher
Despite decades of research on the noradrenergic system, our understanding of its impact on brain function and behavior remains incomplete. Traditional recording techniques are challenging to implement for investigating in vivo noradrenergic activity, due to the relatively small size and the position in the brain of the locus coeruleus (LC), the primary location for noradrenergic neurons. However,
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Capturing a rising star: the emerging role of astrocytes in neural circuit wiring and plasticity-lessons from the visual system. Neurophotonics (IF 5.3) Pub Date : 2023-09-26 David Foubert,Finnley Cookson,Edward S Ruthazer
The increasingly widespread use of calcium imaging to explore the nature of neuronal activity and circuits has unexpectedly revealed the ubiquitous presence and significance of astrocytic activity. Here, we present a brief review of visual system development, placing it in the context of recently identified roles of astrocytes in the modulation of neuronal responses and circuit plasticity, through
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Developing customized NIRS-EEG for infant sleep research: methodological considerations. Neurophotonics (IF 5.3) Pub Date : 2023-09-25 Louisa K Gossé,Paola Pinti,Frank Wiesemann,Clare E Elwell,Emily J H Jones
Significance Studies using simultaneous functional near-infrared spectroscopy (fNIRS)-electroencephalography (EEG) during natural sleep in infancy are rare. Developments for combined fNIRS-EEG for sleep research that ensure optimal comfort as well as good coupling and data quality are needed. Aim We describe the steps toward developing a comfortable, wearable NIRS-EEG headgear adapted specifically
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Linking brain activity across scales with simultaneous opto- and electrophysiology Neurophotonics (IF 5.3) Pub Date : 2023-09-01 Christopher M. Lewis, Adrian Hoffmann, Fritjof Helmchen
The brain enables adaptive behavior via the dynamic coordination of diverse neuronal signals across spatial and temporal scales: from fast action potential patterns in microcircuits to slower patterns of distributed activity in brain-wide networks. Understanding principles of multiscale dynamics requires simultaneous monitoring of signals in multiple, distributed network nodes. Combining optical and
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Enhancing diffuse correlation spectroscopy pulsatile cerebral blood flow signal with near-infrared spectroscopy photoplethysmography. Neurophotonics (IF 5.3) Pub Date : 2023-09-06 Kuan Cheng Wu,Alyssa Martin,Marco Renna,Mitchell Robinson,Nisan Ozana,Stefan A Carp,Maria Angela Franceschini
Significance Combining near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS) allows for quantifying cerebral blood volume, flow, and oxygenation changes continuously and non-invasively. As recently shown, the DCS pulsatile cerebral blood flow index (pCBFi) can be used to quantify critical closing pressure (CrCP) and cerebrovascular resistance (CVRi). Aim Although current DCS
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Toward peripheral nerve mechanical characterization using Brillouin imaging spectroscopy. Neurophotonics (IF 5.3) Pub Date : 2023-08-26 Vsevolod Cheburkanov,Junwei Du,David M Brogan,Mikhail Y Berezin,Vladislav V Yakovlev
Significance Peripheral nerves are viscoelastic tissues with unique elastic characteristics. Imaging of peripheral nerve elasticity is important in medicine, particularly in the context of nerve injury and repair. Elasticity imaging techniques provide information about the mechanical properties of peripheral nerves, which can be useful in identifying areas of nerve damage or compression, as well as
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Warriors of Science: Ukraine's Academic Endeavors. Neurophotonics (IF 5.3) Pub Date : 2023-08-24 Daria Bogatova
A special editorial considers the narratives of four scientists from Ukraine.
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Imaging of post-synaptic membrane trafficking in neuronal dendrites: progress, limitations, and new developments Neurophotonics (IF 5.3) Pub Date : 2023-08-01 David Perrais, Silvia Sposini, Julie Angibaud
Membrane trafficking of post-synaptic cargo is a key determinant of synaptic transmission and synaptic plasticity. We describe here the latest developments in visualizing individual exocytosis and endocytosis events in neurons using pH-sensitive tags. We show how these tools help decipher the spatial and temporal regulation of membrane trafficking steps during synaptic plasticity.
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Addressing annotation and data scarcity when designing machine learning strategies for neurophotonics Neurophotonics (IF 5.3) Pub Date : 2023-08-01 Catherine Bouchard, Renaud Bernatchez, Flavie Lavoie-Cardinal
Machine learning has revolutionized the way data are processed, allowing information to be extracted in a fraction of the time it would take an expert. In the field of neurophotonics, machine learning approaches are used to automatically detect and classify features of interest in complex images. One of the key challenges in applying machine learning methods to the field of neurophotonics is the scarcity
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Automatic and label-free detection of meningioma in dura mater using the combination of multiphoton microscopy and image analysis. Neurophotonics (IF 5.3) Pub Date : 2023-07-10 Na Fang,Zanyi Wu,Rong Chen,Zhongjiang Chen,Limei Zheng,Tingqi Yang,Ling Wang,Xingfu Wang,Dezhi Kang,Jianxin Chen
Significance To prevent meningioma recurrence, it is necessary to detect and remove all corresponding tumors intraoperatively, including those in the adjacent dura mater. Aim Currently, the removal of meningiomas from the dura mater depends solely on cautious visual identification of lesions by a neurosurgeon. Inspired by the requirements for resection, we propose multiphoton microscopy (MPM) based
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Special Section Guest Editorial: Thirty Years of Functional Near-Infrared Spectroscopy. Neurophotonics (IF 5.3) Pub Date : 2023-07-06 David Highton,David Boas,Yasuyo Minagawa,Rickson C Mesquita,Judit Gervain
Functional Near-Infrared Spectroscopy (fNIRS) is a non-invasive optical technique that measures cerebral hemodynamics across multiple regions of interest, and thereby characterises brain functional activation. Since its first description in 1993, fNIRS has undergone substantial developments in hardware, analysis techniques, and applications. Thirty years later, this technique is significantly enchancing
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Impact of averaging fNIRS regional coherence data when monitoring people with long term post-concussion symptoms. Neurophotonics (IF 5.3) Pub Date : 2023-07-04 Ibukunoluwa K Oni,Andrew P Lapointe,Bradley G Goodyear,Chantel T Debert,Jeff F Dunn
Significance Functional near-infrared spectroscopy (fNIRS), with its measure of delta hemoglobin concentration, has shown promise as a monitoring tool for the functional assessment of neurological disorders and brain injury. Analysis of fNIRS data often involves averaging data from several channel pairs in a region. Although this greatly reduces the processing time, it is uncertain how it affects the
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Comparing the segmentation of quantitative phase images of neurons using convolutional neural networks trained on simulated and augmented imagery. Neurophotonics (IF 5.3) Pub Date : 2023-06-30 Eddie M Gil,Zachary A Steelman,Anna Sedelnikova,Joel N Bixler
Significance Quantitative phase imaging (QPI) can visualize cellular morphology and measure dry mass. Automated segmentation of QPI imagery is desirable for tracking neuron growth. Convolutional neural networks (CNNs) have provided state-of-the-art results for image segmentation. Improving the amount and robustness of training data is often crucial to improving CNN output on novel samples, but acquiring
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Applications of near-infrared spectroscopy in neurocritical care. Neurophotonics (IF 5.3) Pub Date : 2023-06-30 Rachel Thomas,Samuel S Shin,Ramani Balu
Significance Acute brain injuries are commonly encountered in the intensive care unit. Alterations in cerebrovascular physiology triggered by the initial insult can lead to neurological worsening, further brain injury, and poor outcomes. Robust methods for assessing cerebrovascular physiology continuously at the bedside are limited. Aim In this review, we aim to assess the potential of near-infrared
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Millimetric scale two-photon Bessel-Gauss beam light sheet microscopy with three-axis isotropic resolution using an axicon lens. Neurophotonics (IF 5.3) Pub Date : 2023-06-23 Cléophace Akitegetse,Thomas Charland,Mireille Quémener,Charles Gora,Véronique Rioux,Michel Piché,Yves De Koninck,Martin Lévesque,Daniel C Côté
Significance Typical light sheet microscopes suffer from artifacts related to the geometry of the light sheet. One main inconvenience is the non-uniform thickness of the light sheet obtained with a Gaussian laser beam. Aim We developed a two-photon light sheet microscope that takes advantage of a thin and long Bessel-Gauss beam illumination to increase the sheet extent without compromising the resolution
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DDeep3M+: adaptive enhancement powered weakly supervised learning for neuron segmentation. Neurophotonics (IF 5.3) Pub Date : 2023-06-23 Rong Xiao,Lei Zhu,Jiangshan Liao,Xinglong Wu,Hui Gong,Jin Huang,Ping Li,Bin Sheng,Shangbin Chen
Significance Robust segmentations of neurons greatly improve neuronal population reconstruction, which could support further study of neuron morphology for brain research. Aim Precise segmentation of 3D neuron structures from optical microscopy (OM) images is crucial to probe neural circuits and brain functions. However, the high noise and low contrast of images make neuron segmentation challenging
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Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion. Neurophotonics (IF 5.3) Pub Date : 2023-06-14 Baoqiang Li,Ji Leng,Ikbal Şencan-Eğilmez,Hajime Takase,Mohammed Ali H Alfadhel,Buyin Fu,Mahnaz Shahidi,Eng H Lo,Ken Arai,Sava Sakadžić
Significance It has been hypothesized that abnormal microcirculation in the retina might predict the risk of ischemic damages in the brain. Direct comparison between the retinal and the cerebral microcirculation using similar animal preparation and under similar experimental conditions would help test this hypothesis. Aim We investigated capillary red-blood-cell (RBC) flux changes under controlled
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HyperOptoNet: a MATLAB-based toolbox for inter-brain neuronal synchrony analysis using fNIRS hyperscanning. Neurophotonics (IF 5.3) Pub Date : 2023-06-13 Gihyoun Lee,Daeun Ro,Seyoung Shin,Yun-Hee Kim
Significance We developed a MATLAB-based toolbox for the analysis of inter-brain synchrony (IBS) and performed an experimental study to confirm its performance. To the best of our knowledge, this is the first toolbox for IBS based on functional near-infrared spectroscopy (fNIRS) hyperscanning data that visually shows the results on two three-dimensional (3D) head models. Aim Research on IBS using fNIRS
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Can pain under anesthesia be measured? Pain-related brain function using functional near-infrared spectroscopy during knee surgery. Neurophotonics (IF 5.3) Pub Date : 2023-06-09 Keerthana Deepti Karunakaran,Ke Peng,Stephen Green,Christine B Sieberg,Arielle Mizrahi-Arnaud,Andrea Gomez-Morad,David Zurakowski,Lyle Micheli,Barry Kussman,David Borsook
Significance Quantitative measurement of perisurgical brain function may provide insights into the processes contributing to acute and chronic postsurgical pain. Aim We evaluate the hemodynamic changes in the prefrontal cortex (medial frontopolar cortex/mFPC and lateral prefrontal cortex) and the primary somatosensory cortex/S1 using functional near-infrared spectroscopy (fNIRS) in 18 patients (18
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Using depth-enhanced diffuse correlation spectroscopy and near-infrared spectroscopy to isolate cerebral hemodynamics during transient hypotension. Neurophotonics (IF 5.3) Pub Date : 2023-06-05 Leena N Shoemaker,Daniel Milej,Jigneshkumar Mistry,Keith St Lawrence
Significance Combining diffuse correlation spectroscopy (DCS) and near-infrared spectroscopy (NIRS) permits simultaneous monitoring of multiple cerebral hemodynamic parameters related to cerebral autoregulation; however, interpreting these optical measurements can be confounded by signal contamination from extracerebral tissue. Aim We aimed to evaluate extracerebral signal contamination in NIRS/DCS
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Site-specific effects of 800- and 850-nm forehead transcranial photobiomodulation on prefrontal bilateral connectivity and unilateral coupling in young adults. Neurophotonics (IF 5.3) Pub Date : 2023-06-05 Sadra Shahdadian,Xinlong Wang,Shu Kang,Caroline Carter,Hanli Liu
Significance Transcranial photobiomodulation (tPBM) is a noninvasive neuromodulation method that facilitates the improvement of human cognition. However, limited information is available in the literature on the wavelength- and site-specific effects of prefrontal tPBM. Moreover, 2-channel broadband near-infrared spectroscopy (2-bbNIRS) is a new approach for quantifying infra-slow oscillations (ISO;
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Recent advances and challenges in the use of CRISPR/Cas9 genome editing for understanding neuronal cell biology Neurophotonics (IF 5.3) Pub Date : 2023-06-01 Harold D. MacGillavry
The ability to accurately map and manipulate the dynamic subcellular distribution of proteins is key for a mechanistic understanding of neuronal functioning. Current fluorescence microscopy techniques provide access to subcellular protein organization at increasing resolution but are often restricted by the availability of methods that reliably label endogenous proteins. Excitingly, recent development
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Functional near-infrared spectroscopy neurofeedback of dorsolateral prefrontal cortex enhances human spatial working memory. Neurophotonics (IF 5.3) Pub Date : 2023-06-01 Keshuang Li,Jinhao Yang,Benjamin Becker,Xianchun Li
Significance Spatial working memory (SWM) is essential for daily life and deficits in this domain represent a common impairment across aging and several mental disorders. Impaired SWM has been closely linked to dysregulations in dorsolateral prefrontal cortex (DLPFC) activation. Aim The present study evaluates the feasibility and maintenance of functional near-infrared spectroscopy neurofeedback (fNIRS-NF)
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Test-retest reliability of functional near-infrared spectroscopy during a finger-tapping and postural task in healthy older adults. Neurophotonics (IF 5.3) Pub Date : 2023-05-26 Veerle de Rond,Moran Gilat,Nicholas D'Cruz,Femke Hulzinga,Jean-Jacques Orban de Xivry,Alice Nieuwboer
Significance Functional near-infrared spectroscopy (fNIRS) is increasingly employed in studies requiring repeated measurements, yet test-retest reliability is largely unknown. Aim To investigate test-retest reliability during a postural and a finger-tapping task with and without cap-removal. Approach Twenty healthy older adults performed a postural and a finger-tapping task. The tasks were repeated
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Effects of formalin fixation on polarimetric properties of brain tissue: fresh or fixed? Neurophotonics (IF 5.3) Pub Date : 2023-05-24 Romain Gros,Omar Rodríguez-Núñez,Leonard Felger,Stefano Moriconi,Richard McKinley,Angelo Pierangelo,Tatiana Novikova,Erik Vassella,Philippe Schucht,Ekkehard Hewer,Theoni Maragkou
Significance Imaging Mueller polarimetry (IMP) appears as a promising technique for real-time delineation of healthy and neoplastic tissue during neurosurgery. The training of machine learning algorithms used for the image post-processing requires large data sets typically derived from the measurements of formalin-fixed brain sections. However, the success of the transfer of such algorithms from fixed
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Short-separation regression incorporated diffuse optical tomography image reconstruction modeling for high-density functional near-infrared spectroscopy. Neurophotonics (IF 5.3) Pub Date : 2023-05-23 Yuanyuan Gao,De'Ja Rogers,Alexander von Lühmann,Antonio Ortega-Martinez,David A Boas,Meryem Ayşe Yücel
Significance Short-separation (SS) regression and diffuse optical tomography (DOT) image reconstruction, two widely adopted methods in functional near-infrared spectroscopy (fNIRS), were demonstrated to individually facilitate the separation of brain activation and physiological signals, with further improvement using both sequentially. We hypothesized that doing both simultaneously would further improve
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Optimizing a two-layer method for hybrid diffuse correlation spectroscopy and frequency-domain diffuse optical spectroscopy cerebral measurements in adults. Neurophotonics (IF 5.3) Pub Date : 2023-05-23 Rodrigo Menezes Forti,Giovani Grisotti Martins,Wesley Boehs Baker,Rickson C Mesquita
Significance The sensitivity to extracerebral tissues is a well-known confounder of diffuse optics. Two-layer (2L) head models can separate cerebral signals from extracerebral artifacts, but they also carry the risk of crosstalk between fitting parameters. Aim We aim to implement a constrained 2L head model for hybrid diffuse correlation spectroscopy (DCS) and frequency-domain diffuse optical spectroscopy
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Behavioral modeling and neuroimaging of impaired risky decision making in patients with chronic musculoskeletal pain. Neurophotonics (IF 5.3) Pub Date : 2023-05-18 Xinglin Zeng,Wen Tang,Fei Gao,Ziyue Tang,Zhou Zhang,Juan Zhang,Meng Du,Zhiyi Chen,Xueli Chen,Zhen Yuan
Significance Performance during risky decision making is one of the essential cognitive functions that is impaired in several psychiatric disorders including addiction. However, the cognitive mechanism and neural correlates underlying risky decision making in chronic pain patients are unclear. To our knowledge, this study is among the first to construct computational models to detect the underlying
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Wearable, high-density fNIRS and diffuse optical tomography technologies: a perspective. Neurophotonics (IF 5.3) Pub Date : 2023-05-17 Ernesto E Vidal-Rosas,Alexander von Lühmann,Paola Pinti,Robert J Cooper
Recent progress in optoelectronics has made wearable and high-density functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) technologies possible for the first time. These technologies have the potential to open new fields of real-world neuroscience by enabling functional neuroimaging of the human cortex at a resolution comparable to fMRI in almost any environment and population
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Changes in functional neuroimaging measures as novices gain proficiency on the fundamentals of laparoscopic surgery suturing task Neurophotonics (IF 5.3) Pub Date : 2023-05-01 Yaoyu Fu, Pushpinder Walia, Steven D. Schwaitzberg, Xavier Intes, Suvranu De, Anirban Dutta, Lora Cavuoto
SignificanceAs trainees practice fundamental surgical skills, they typically rely on performance measures such as time and errors, which are limited in their sensitivity.AimThe goal of our study was to evaluate the use of portable neuroimaging measures to map the neural processes associated with learning basic surgical skills.ApproachTwenty-one subjects completed 15 sessions of training on the fundamentals
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Intrinsic, widefield optical imaging of hemodynamics in rodent models of Alzheimer’s disease and neurological injury Neurophotonics (IF 5.3) Pub Date : 2023-05-01 Christian Crouzet, Thinh Phan, Robert H. Wilson, Teo Jeon Shin, Bernard Choi
The complex cerebrovascular network is critical to controlling local cerebral blood flow (CBF) and maintaining brain homeostasis. Alzheimer’s disease (AD) and neurological injury can result in impaired CBF regulation, blood–brain barrier breakdown, neurovascular dysregulation, and ultimately impaired brain homeostasis. Measuring cortical hemodynamic changes in rodents can help elucidate the complex
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Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo Neurophotonics (IF 5.3) Pub Date : 2023-05-01 Ruijie Li, Sibo Wang, Jing Lyu, Ke Chen, Xiaxin Sun, Junjie Huang, Pei Sun, Susu Liang, Min Li, Mengke Yang, Hongbang Liu, Shaoqun Zeng, Xiaowei Chen, Longhui Li, Hongbo Jia, Zhenqiao Zhou
SignificanceThe studying of rapid neuronal signaling across large spatial scales in intact, living brains requires both high temporal resolution and versatility of the measurement device.AimWe introduce a high-speed two-photon microscope based on a custom-built acousto-optic deflector (AOD). This microscope has a maximum line scan frequency of 400 kHz and a maximum frame rate of 10,000 frames per second
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Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy Neurophotonics (IF 5.3) Pub Date : 2023-05-01 Stéphane Bancelin, Luc Mercier, Johannes Roos, Mohamed Belkadi, Thomas Pfeiffer, Sun Kwang Kim, U. Valentin Nägerl
SignificanceStimulated emission depletion (STED) microscopy has been used to address a wide range of neurobiological questions in optically well-accessible samples, such as cell culture or brain slices. However, the application of STED to deeply embedded structures in the brain of living animals remains technically challenging.AimIn previous work, we established chronic STED imaging in the hippocampus
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Light, questions, probes: Valentina Emiliani on combining disciplines for neurophotonics Neurophotonics (IF 5.3) Pub Date : 2023-05-01 Amanda Foust
Valentina Emiliani, CNRS research director at the Vision Institute in Paris, discusses her pioneering work in neurophotonics, in an interview with fellow Neurophotonics Editorial Board Member Amanda Foust, Senior Lecturer in Biomedical Engineering at University College London.
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Pupil engineering for extended depth-of-field imaging in a fluorescence miniscope Neurophotonics (IF 5.3) Pub Date : 2023-05-01 Joseph Greene, Yujia Xue, Jeffrey Alido, Alex Matlock, Guorong Hu, Kivilcim Kiliç, Ian Davison, Lei Tian
SignificanceFluorescence head-mounted microscopes, i.e., miniscopes, have emerged as powerful tools to analyze in-vivo neural populations but exhibit a limited depth-of-field (DoF) due to the use of high numerical aperture (NA) gradient refractive index (GRIN) objective lenses.AimWe present extended depth-of-field (EDoF) miniscope, which integrates an optimized thin and lightweight binary diffractive
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Predicting poststroke dyskinesia with resting-state functional connectivity in the motor network Neurophotonics (IF 5.3) Pub Date : 2023-04-01 Shuoshu Lin, Dan Wang, Haojun Sang, Hongjun Xiao, Kecheng Yan, Dongyang Wang, Yizheng Zhang, Li Yi, Guangjian Shao, Zhiyong Shao, Aoran Yang, Lei Zhang, Jinyan Sun
SignificanceMotor function evaluation is essential for poststroke dyskinesia rehabilitation. Neuroimaging techniques combined with machine learning help decode a patient’s functional status. However, more research is needed to investigate how individual brain function information predicts the dyskinesia degree of stroke patients.AimWe investigated stroke patients’ motor network reorganization and proposed
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Reproducibility of infant fNIRS studies: a meta-analytic approach (Erratum) Neurophotonics (IF 5.3) Pub Date : 2023-04-01 Jessica Gemignani, Irene de la Cruz-Pavía, Anna Martinez, Caroline Nallet, Alessia Pasquini, Gaia Lucarini, Francesca Cavicchiolo, Judit Gervain
The erratum documents correction of erroneous captions for Figs. 5-7.
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Using functional near-infrared spectroscopy to study the early developing brain: future directions and new challenges Neurophotonics (IF 5.3) Pub Date : 2023-04-01 Judit Gervain, Yasuyo Minagawa, Lauren Emberson, Sarah Lloyd-Fox
SignificanceFunctional near-infrared spectroscopy (fNIRS) is a frequently used neuroimaging tool to explore the developing brain, particularly in infancy, with studies spanning from birth to toddlerhood (0 to 2 years). We provide an overview of the challenges and opportunities that the developmental fNIRS field faces, after almost 25 years of research.AimWe discuss the most recent advances in fNIRS
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Preictal neuronal and vascular activity precedes the onset of childhood absence seizure: direct current potential shifts and their correlation with hemodynamic activity Neurophotonics (IF 5.3) Pub Date : 2023-04-01 Mina Nourhashemi, Mahdi Mahmoudzadeh, Claire Heberle, Fabrice Wallois
Significance, AimsThe neurovascular mechanisms underlying the initiation of absence seizures and their dynamics are still not well understood. The objective of this study was to better noninvasively characterize the dynamics of the neuronal and vascular network at the transition from the interictal state to the ictal state of absence seizures and back to the interictal state using a combined electroencephalography
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Shedding light on functional near-infrared spectroscopy and open science practices Neurophotonics (IF 5.3) Pub Date : 2023-04-01 Caroline Kelsey, Jebediah Taylor, Laura Pirazzoli, Renata Di Lorenzo, Eileen F. Sullivan, Charles A. Nelson
Open science practices work to increase methodological rigor, transparency, and replicability of published findings. We aim to reflect on what the functional near-infrared spectroscopy (fNIRS) community has done to promote open science practices in fNIRS research and set goals to accomplish over the next 10 years.