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Ethnic disparities and its association between epicardial adipose tissue thickness and cardiometabolic parameters Adipocyte (IF 3.3) Pub Date : 2024-02-19 WAS Mohamed, KN Ishak, N Baharum, NAZ Zainudin, Han Yin Lim, MFM Noh, WAW Ahmad, H Zaman Huri, ASM Zuhdi, S Sukahri, K Govindaraju, AH Abd Jamil
Excessive deposit of epicardial adipose tissue (EAT) were recently shown to be positively correlated with cardiovascular disease (CVD). This study aims to investigate the thickness of EAT and its a...
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Function of NAD metabolism in white adipose tissue: lessons from mouse models Adipocyte (IF 3.3) Pub Date : 2024-02-15 So Young Kwon, Yoon Jung Park
Nicotinamide Adenine Dinucleotide (NAD) is an endogenous substance in redox reactions and regulates various functions in metabolism. NAD and its precursors are known for their anti-ageing and anti-...
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Leptin secreted by adipocytes promotes EMT transition and endometrial cancer progression via the JAK2/STAT3 signalling pathway Adipocyte (IF 3.3) Pub Date : 2023-12-13 Lifan Shen, Chen Zhang, Kaiying Cui, Xin Liang, Genhai Zhu, Lan Hong
Endometrial cancer is a malignant tumour with a high incidence and mortality rate, and obesity is one of the most significant risk factors for the disease. However, it remains unclear whether lepti...
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Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells Adipocyte (IF 3.3) Pub Date : 2023-12-08 Paula Boczki, Marco Colombo, Juliane Weiner, Inka Rapöhn, Martin Lacher, Wieland Kiess, Martha Hanschkow, Antje Körner, Kathrin Landgraf
S-adenosyl-homocysteine-hydrolase (AHCY) plays an important role in the methionine cycle regulating cellular methylation levels. AHCY has been reported to influence proliferation and differentiatio...
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Ojeok-san enhances platinum sensitivity in ovarian cancer by regulating adipocyte paracrine IGF1 secretion Adipocyte (IF 3.3) Pub Date : 2023-11-22 Jiong Ma, Junyan Li, Xuejun Chen, Yanyan Ma
Platinum is a commonly used drug for ovarian cancer (OvCa) treatment, but drug resistance limits its clinical application. This study intended to delineate the effects of adipocytes on platinum res...
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Adipogenic characterization of immortalized CD55+ progenitor cells from human white adipose tissue Adipocyte (IF 3.3) Pub Date : 2023-11-20 Morgane Couchet, Hui Gao, Felix Klingelhuber, Jutta Jalkanen, Thais De Castro Barbosa, Muhmmad Omar-Hmeadi, Lucas Massier, Natalie Krahmer, Niklas Mejhert, Mikael Rydén
Mature adipocytes are notoriously difficult to study ex vivo and alternative cell culture systems have therefore been developed. One of the most common models are human adipose progenitor cells (hA...
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IL-27 increases energy storage in white adipocytes by enhancing glucose uptake and fatty acid esterification Adipocyte (IF 3.3) Pub Date : 2023-11-10 Chiara Scaffidi, Annie Srdic, Daniel Konrad, Stephan Wueest
The cytokine interleukin (IL)-27 has been reported to induce thermogenesis in white adipocytes. However, it remains unknown whether IL-27-mediated adipocyte energy dissipation is paralleled by an e...
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Bioactive peptides PDBSN improve mitochondrial function and suppression the oxidative stress in human adiposity cells Adipocyte (IF 3.3) Pub Date : 2023-11-09 Huiping Shen, Yong Lei, Wen Xie, Tieliang Ma, Li Bao, Qin Gao, Bingyu Chen, Biao Dai, Dani Qin
Introduction: Mitochondria are essential for generating cellular energy and are significant in the pathogenesis of obesity. Peptide PDBSN has been demonstrated to inhibit the adipogenic differentia...
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Integrative bioinformatics analysis to screen key genes and signalling pathways related to ferroptosis in obesity Adipocyte (IF 3.3) Pub Date : 2023-10-25 Ming-Ke Li, Chang Xing, Lan-Qing Ma
Ferroptosis is closely associated with the development of disease in the body. However, there are few studies on ferroptosis-related genes (FRGs) in obesity. Therefore, key genes and signalling pat...
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Changes of insulin receptors in high fat and high glucose diet mice with insulin resistance Adipocyte (IF 3.3) Pub Date : 2023-10-13 Chen Lei, Jing Wang, Xin Li, Yuan-Yuan Mao, Jian-Qun Yan
This study aimed to observe the expression of insulin-signaling molecules in different organs of mice with insulin resistance (IR). Firstly, mice were fed a high-fat and high-sugar diet (HF group) ...
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Matrix density regulates adipocyte phenotype Adipocyte (IF 3.3) Pub Date : 2023-10-10 Alexander Ky, Atticus J. McCoy, Carmen G. Flesher, Nicole E. Friend, Jie Li, Kore Akinleye, Christopher Patsalis, Carey N. Lumeng, Andrew J. Putnam, Robert W. O’Rourke
Alterations of the extracellular matrix contribute to adipose tissue dysfunction in metabolic disease. We studied the role of matrix density in regulating human adipocyte phenotype in a tunable hyd...
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Regulatory effects and mechanisms of exercise on activation of brown adipose tissue (BAT) and browning of white adipose tissue (WAT) Adipocyte (IF 3.3) Pub Date : 2023-10-09 Haijun Dong, Man Qin, Peng Wang, Shufan Li, Xing Wang
Exercise is a universally acknowledged and healthy way to reducing body weight. However, the roles and mechanisms of exercise on metabolism of adipose tissue remain largely unclear. Adipose tissues...
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Overexpression of Plg-RKT protects against adipose dysfunction and dysregulation of glucose homeostasis in diet-induced obese mice Adipocyte (IF 3.3) Pub Date : 2023-09-04 Lindsey A. Miles, Hongdong Bai, Sagarika Chakrabarty, Nagyung Baik, Yuqing Zhang, Robert J. Parmer, Fahumiya Samad
The plasminogen receptor, Plg-RKT, is a unique cell surface receptor that is broadly expressed in cells and tissues throughout the body. Plg-RKT localizes plasminogen on cell surfaces and promotes ...
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Perilipin-1 immunostaining improves semi-automated digital quantitation of bone marrow adipocytes in histological bone sections Adipocyte (IF 3.3) Pub Date : 2023-08-30 Nicko Widjaja, Niki Jalava, Yimeng Chen, Kaisa K. Ivaska
ABSTRACT Bone marrow adipocytes (BMAds) are not just passive fillers inside the bone marrow compartment but respond to various metabolic changes. Assessment of those responses requires evaluation of the number of BMAds and their morphology for which laborious and error-prone manual histological analysis remains the most widely used method. Here, we report an alternative image analysis strategy to semi-automatically
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The relationship between adipose tissue RAAS activity and the risk factors of prediabetes: a systematic review and meta-analysis Adipocyte (IF 3.3) Pub Date : 2023-08-31 Bongeka Cassandra Mkhize, Palesa Mosili, Phikelelani Sethu Ngubane, Andile Khathi
ABSTRACT The exponential increase in the prevalence of prediabetes has become a global concern due to the comorbidities and mortality rates that are positively associated with it. The incidence of prediabetes is directly proportional to the prevalence of comorbidities with risk factors such as insulin resistance, adiposity, lipotoxicity, obesity and the alteration of the renin-angiotensin-aldosterone
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Emerging technologies in adipose tissue research Adipocyte (IF 3.3) Pub Date : 2023-08-20 Dimiter Avtanski, Nikola Hadzi-Petrushev, Slavica Josifovska, Mitko Mladenov, Varun Reddy
ABSTRACT Technologies are transforming the understanding of adipose tissue as a complex and dynamic tissue that plays a critical role in energy homoeostasis and metabolic health. This mini-review provides a brief overview of the potential impact of novel technologies in biomedical research and aims to identify areas where these technologies can make the most significant contribution to adipose tissue
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A study to investigate genetic factors associated with weight gain in people with diabetes: analysis of polymorphisms in four relevant genes Adipocyte (IF 3.3) Pub Date : 2023-08-15 Adrian Heald, Rui Qin, Camila M. Loureiro, Richard Williams, Chloe Devaney Dopson, J Martin Gibson, Ram Prakash Narayanan, Helene Fachim, Kevin McCay, William Ollier
ABSTRACT Background Weight change is often seen in people with diabetes. We investigated the effects of genes associated with weight change/glucose handling/insulin-signalling. Materials/methods DNA from diabetes individuals and non-diabetes individuals, plus clinical data, were available from the DARE study (n = 379 individuals: T1D n = 111; T2D n = 222; controls n = 46). Weight gain was assessed
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CABLES1 expression is reduced in human subcutaneous adipose tissue in obesity and type 2 diabetes but may not directly impact adipocyte glucose and lipid metabolism Adipocyte (IF 3.3) Pub Date : 2023-08-09 Susanne Hetty, Milica Vranic, Prasad G Kamble, Martin H Lundqvist, Maria J Pereira, Jan W Eriksson
ABSTRACT Cdk5 and Abl enzyme substrate 1 (CABLES1) is a cell cycle regulator that has previously been identified as a candidate gene for obesity-related phenotypes, but little is known about its role in adipose tissue metabolism. In this study, we explore the role of CABLES1 in obesity and type 2 diabetes (T2D) in human subcutaneous adipose tissue (SAT). We performed gene expression analysis of SAT
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Brown adipocyte and browning thermogenesis: metabolic crosstalk beyond mitochondrial limits and physiological impacts Adipocyte (IF 3.3) Pub Date : 2023-07-24 Van Thi-Tuong Nguyen, Vuong Van Vu, Phuc Van Pham
ABSTRACT Brown adipocytes were proposed to reverse metabolic conditions such as obesity and diabetes, which make them potential for therapeutic applications. Brown adipocytes and browning process are capable of thermogenesis, the uncoupling metabolism which allows them to promote balanced energy expenditure, a fundamental mechanism for improving metabolic disorders. Thermogenesis process is not only
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Integrative analysis of Mendelian randomization and gene expression profiles reveals a null causal relationship between adiponectin and diabetic retinopathy Adipocyte (IF 3.3) Pub Date : 2023-07-17 Ao Zhang, Hui Wu, Chi Wang, Suyan Tian
ABSTRACT Observational studies have been conducted to investigate the correlation between adiponectin and diabetic retinopathy (DR), but no consistent relationship has been established. In this study, we employed an integrative analysis that combined Mendelian randomization (MR) and bioinformatics analyses to comprehensively explore the association between DR and adiponectin, aiming to provide a unified
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Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells Adipocyte (IF 3.3) Pub Date : 2023-07-20 Danang Dwi Cahyadi, Tomoko Warita, Nanami Irie, Kana Mizoguchi, Jiro Tashiro, Yoshinao Z. Hosaka, Katsuhiko Warita
ABSTRACT Normalization is a crucial step in gene expression analysis to avoid misinterpretation. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression of 10 candidate housekeeping genes in non-differentiated (ND) and differentiated (DI) 3T3-L1 cells on days 5 and 10. We used geNorm, NormFinder, BestKeeper, RefFinder, and the ∆Ct method to evaluate expression
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MRI-measured periprostatic adipose tissue volume as a prognostic predictor in prostate cancer patients undergoing laparoscopic radical prostatectomy Adipocyte (IF 3.3) Pub Date : 2023-04-25 Tianyu Xiong, Fang Cao, Guangyi Zhu, Xiaobo Ye, Yun Cui, Nianzeng Xing, Huibo Zhang, Yinong Niu
ABSTRACT In this study, we evaluated the association between the PPAT volume and the prognosis of PCa patients after LRP. We retrospectively analysed data of 189 PCa patients who underwent LRP in Beijing Chaoyang Hospital. Volumes of PPAT and prostate were measured by magnetic resonance imaging (MRI), and normalized PPAT volume was computed (PPAT volume divided by prostate volume). Patients were then
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Adipokines in glucose and lipid metabolism Adipocyte (IF 3.3) Pub Date : 2023-04-19 Xueqing Wang, Siwen Zhang, Zhuo Li
ABSTRACT Adipokines are proteins secreted by adipose tissue to regulate glucolipid metabolism and play vital roles in our body. Different adipokines have more than one endocrine function and be divided into several different categories according to their functions, including adipokines involved in glucolipid metabolism, the inflammatory response, insulin action, activation of brown adipose tissue (BAT)
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TRAF6 promotes osteogenesis in ADSCs through Raf-Erk-Merk-Hif1-a pathway Adipocyte (IF 3.3) Pub Date : 2023-04-02 Xiang-Dong Liu, Jian Zheng, Shuang Song, Zi-Jun Chen, Yu-Xi Wang, Si-Jia Zhang, Guan-Hua Zhang, Qun Lu, Ying-Liang Song
ABSTRACT Critical-size defects (CSDs) are challenging oral clinical issues that need to be solved. Adipose-derived mesenchymal stem cells (ADSCs) and gene therapy offer a new target to solve these issues. Consequently, ADSCs attract more and more attention because of advantages such as easy obtainability and no ethical concerns. TNF receptor-associated factor 6 (TRAF6) is a significant binding protein
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In vitro studies of the renin-angiotensin system in human adipose tissue/adipocytes and possible relationship to SARS-CoV-2: a scoping review Adipocyte (IF 3.3) Pub Date : 2023-03-27 Ryan Ting, Heidi Dutton, Alexander Sorisky
ABSTRACT The renin-angiotensin system (RAS) operates within adipose tissue. Obesity-related changes can affect adipose RAS, predisposing to hypertension, type 2 diabetes, and possibly severe COVID-19. We evaluated the in vitro research on human adipose RAS and identified gaps in the literature. Medline (Ovid), Embase (Ovid), Web of Science, Scopus, and 1findr were searched to identify relevant studies
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An optimised protocol for the investigation of insulin signalling in a human cell culture model of adipogenesis Adipocyte (IF 3.3) Pub Date : 2023-02-27 Jonathan M. Gamwell, Keanu Paphiti, Leanne Hodson, Fredrik Karpe, Katherine E. Pinnick, Marijana Todorčević
ABSTRACT While there is no standardized protocol for the differentiation of human adipocytes in culture, common themes exist in the use of supra-physiological glucose and hormone concentrations, and an absence of exogenous fatty acids. These factors can have detrimental effects on some aspects of adipogenesis and adipocyte function. Here, we present methods for modifying the adipogenic differentiation
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An optimized method for Oil Red O staining with the salicylic acid ethanol solution Adipocyte (IF 3.3) Pub Date : 2023-02-24 Junbao Du, Li Zhao, Quan Kang, Yun He, Yang Bi
ABSTRACT Oil Red O (ORO) staining is a commonly used experimental technique to detect lipid content in cells or tissues. Freshly prepared ORO in 60% isopropanol is the most widely used method at present. However, isopropanol is volatile and harmful to the human body. It will also affect the interpretation of the results due to the formation of crystals and non-specific diffuse staining. In this paper
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Highly-expressed lncRNA FOXD2-AS1 in adipose mesenchymal stem cell derived exosomes affects HaCaT cells via regulating miR-185-5p/ROCK2 axis Adipocyte (IF 3.3) Pub Date : 2023-02-12 Huanchao Chang, Junliang Chen, Kun Ding, Tianling Cheng, Shengjian Tang
ABSTRACT The healing of skin wounds is a highly coordinated multi-step process that occurs after trauma including surgical incisions, thermal burns, and chronic ulcers. In this study, the authors investigated lncRNA FOXD2-AS1 function in adipose mesenchymal exosomes from ADMSCs that were successfully extracted. Highly expressed lncRNA FOXD2-AS1 in ADMSCs-exosomes accelerated HaCaT cell migration and
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miR136 regulates proliferation and differentiation of small tail han sheep preadipocytes Adipocyte (IF 3.3) Pub Date : 2023-02-10 Man Luo, Lin Wang, Cheng Xiao, Mengsi Zhou, Minghui Li, Hongjuan Li
ABSTRACT Low meat performance is the defect of Small Tail Han sheep. Intramuscular fat affects meat quality and largely determined by adipogenesis. In previous study, miR136 was showed one of differentially expressed microRNAs between preadipocytes and mature adipocytes of Small Tail Han sheep but its role in adipogenesis is still not elucidated. Here, we investigated the effect of miR136 on adipogenesis
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Identification of major hub genes involved in high-fat diet-induced obese visceral adipose tissue based on bioinformatics approach Adipocyte (IF 3.3) Pub Date : 2023-02-01 Yu Jiang, Rui Zhang, Jia-Qi Guo, Ling-Lin Qian, Jing-Jing Ji, Ya Wu, Zhen-Jun Ji, Zi-Wei Yang, Yao Zhang, Xi Chen, Gen-Shan Ma, Yu-Yu Yao
ABSTRACT High-fat diet (HFD) can cause obesity, inducing dysregulation of the visceral adipose tissue (VAT). This study aimed to explore potential biological pathways and hub genes involved in obese VAT, and for that, bioinformatic analysis of multiple datasets was performed. The expression profiles (GSE30247, GSE167311 and GSE79434) were downloaded from Gene Expression Omnibus. Overlapping differentially
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miR-26a-5p Regulates Adipocyte Differentiation via Directly Targeting ACSL3 in Adipocytes Adipocyte (IF 3.3) Pub Date : 2023-01-29 Ning Ding, Wenwen Wang, Jun Teng, Yongqing Zeng, Qin Zhang, Licai Dong, Hui Tang
ABSTRACT Preadipocytes become mature adipocytes after proliferation and differentiation, and although many genes and microRNAs have been identified in intramuscular fat, their physiological function and regulatory mechanisms remain largely unexplored. miR-26a-5p has been reported to be related to fat deposition, but its effect on porcine preadipocyte differentiation has not been explored. In this study
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Lumican modulates adipocyte function in obesity-associated type 2 diabetes Adipocyte (IF 3.3) Pub Date : 2022-12-06 Clarissa Strieder-Barboza, Carmen G. Flesher, Lynn M. Geletka, Tad Eichler, Olukemi Akinleye, Alexander Ky, Anne P. Ehlers, Carey N. Lumeng, Robert W. O’Rourke
ABSTRACT Obesity-associated type 2 diabetes (DM) leads to adipose tissue dysfunction. Lumican is a proteoglycan implicated in obesity, insulin resistance (IR), and adipocyte dysfunction. Using human visceral adipose tissue (VAT) from subjects with and without DM, we studied lumican effects on adipocyte function. Lumican was increased in VAT and adipocytes in DM. Lumican knockdown in adipocytes decreased
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Plasminogen-derived peptide promotes adipogenic differentiation of preadipocytes in vitro and in vivo Adipocyte (IF 3.3) Pub Date : 2022-11-30 Hea Jung Yang, Jong-Ho Kim, Jung Hee Shim, Chan Yeong Heo
ABSTRACT Soft tissue defects caused by adipose tissue loss can result in various conditions such as lipodystrophy in congenital diseases, trauma secondary to ageing, and mastectomy in breast cancer; fat grafting is commonly performed to restore these defects. Although various enrichment strategies have been studied, novel therapeutics that are cost-effective, safe, technologically easy to manufacture
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MRI-measured adipose features as predictive factors for detection of prostate cancer in males undergoing systematic prostate biopsy: a retrospective study based on a Chinese population Adipocyte (IF 3.3) Pub Date : 2022-11-24 Tianyu Xiong, Fang Cao, Guangyi Zhu, Xiaobo Ye, Yun Cui, Huibo Zhang, Yinong Niu
ABSTRACT In this study, we retrospectively evaluated the data of 901 men undergoing ultrasonography-guided systematic prostate biopsy between March 2013 and May 2022. Adipose features, including periprostatic adipose tissue (PPAT) thickness and subcutaneous fat thickness, were measured using MRI before biopsy. Prediction models of all PCa and clinically significant PCa (csPCa) (Gleason score higher
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Associations of GHR, IGF-1 and IGFBP-3 expression in adipose tissue cells with obesity-related alterations in corresponding circulating levels and adipose tissue function in children Adipocyte (IF 3.3) Pub Date : 2022-11-22 Elena Kempf, Kathrin Landgraf, Tim Vogel, Ulrike Spielau, Robert Stein, Matthias Raschpichler, Jürgen Kratzsch, Wieland Kiess, Juraj Stanik, Antje Körner
ABSTRACT Components of the growth hormone (GH) axis, such as insulin-like growth factor-1 (IGF-1), IGF-1 binding protein-3 (IGFBP-3), GH receptor (GHR) and GH-binding protein (GHBP), regulate growth and metabolic pathways. Here, we asked if serum levels of these factors are altered with overweight/obesity and if this is related to adipose tissue (AT) expression and/or increased fat mass. Furthermore
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Identification and characterization of novel abdominal and pelvic brown adipose depots in mice Adipocyte (IF 3.3) Pub Date : 2022-10-19 Ana M. Mesa, Theresa I. Medrano, Vijay K. Sirohi, William H. Walker, Richard D. Johnson, Sergei G. Tevosian, Angie M. Adkin, Paul S. Cooke
ABSTRACT Brown adipose tissue (BAT) generates heat through non-shivering thermogenesis, and increasing BAT amounts or activity could facilitate obesity treatment and provide metabolic benefits. In mice, BAT has been reported in perirenal, thoracic and cranial sites. Here, we describe new pelvic and lower abdominal BAT depots located around the urethra, internal reproductive and urinary tract organs
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Dipeptidyl peptidase-4 cell surface expression marks an abundant adipose stem/progenitor cell population with high stemness in human white adipose tissue Adipocyte (IF 3.3) Pub Date : 2022-10-03 Florian M Hatzmann, Sonja Großmann, Petra Waldegger, G Jan Wiegers, Markus Mandl, Tina Rauchenwald, Gerhard Pierer, Werner Zwerschke
ABSTRACT The capacity of adipose stem/progenitor cells (ASCs) to undergo self-renewal and differentiation is crucial for adipose tissue homoeostasis, regeneration and expansion. However, the heterogeneous ASC populations of the adipose lineage constituting adipose tissue are not precisely known. In the present study, we demonstrate that cell surface expression of dipeptidyl peptidase-4 (DPP4)/cluster
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SPRY4 promotes adipogenic differentiation of human mesenchymal stem cells through the MEK–ERK1/2 signaling pathway Adipocyte (IF 3.3) Pub Date : 2022-09-15 Na Li, Yunfei Chen, Haiyan Wang, Jing Li, Robert Chunhua Zhao
ABSTRACT Obesity is a chronic metabolic disorder characterized by the accumulation of excess fat in the body. Preventing and controlling obesity by inhibiting the adipogenic differentiation of mesenchymal stem cells (MSCs) and thereby avoiding the increase of white adipose tissue is safe and effective. Recent studies have demonstrated that Sprouty proteins (SPRYs) are involved in cell differentiation
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Adipose-derived mesenchymal stem cell-secreted extracellular vesicles alleviate non-alcoholic fatty liver disease via delivering miR-223-3p Adipocyte (IF 3.3) Pub Date : 2022-09-12 Qinghui Niu, Ting Wang, Zhiqiang Wang, Feng Wang, Deyu Huang, Huali Sun, Hanyun Liu
ABSTRACT Increasing studies have identified the potential of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) in non-alcoholic fatty liver disease (NAFLD) treatment. Hence, we further focused on the potential of adipose-derived MSC (ADSC)-EVs in NAFLD by delivering miR-223-3p. The uptake of isolated ADSC-EVs by hepatocytes was assessed, and the expression of miR-223-3p in ADSC-EVs and
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Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway Adipocyte (IF 3.3) Pub Date : 2022-09-02 Jialin Sun, Ping Leng, Xiao Li, Qie Guo, Jun Zhao, Yu Liang, Xiaolei Zhang, Xue Yang, Jing Li
ABSTRACT Mitochondrial dysfunction is associated with insulin resistance and type 2 diabetes (T2DM). Decreased mitochondrial abundance and function were found in white adipose tissue (WAT) of T2DM patients. Therefore, promoting WAT mitochondrial biogenesis and improving adipocyte metabolism may be strategies to prevent and reverse T2DM. Salvianolic acid A (SAA) has been found to exert anti-diabetic
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Comparative transcriptomic analysis of rabbit interscapular brown adipose tissue whitening under physiological conditions Adipocyte (IF 3.3) Pub Date : 2022-08-29 Lei Li, Qian Wan, Qiaoyun Long, Tao Nie, Shiting Zhao, Liufeng Mao, Chuanli Cheng, Chao Zou, Kerry Loomes, Aimin Xu, Liangxue Lai, Xin Liu, Ziyuan Duan, Xiaoyan Hui, Donghai Wu
ABSTRACT Interscapular brown adipose tissue (iBAT) of both rabbits and humans exhibits a similar whitening phenomenon under physiological conditions. However, a detailed characterization of iBAT whitening in them is still lacking. Here, we chose rabbits as a model to gain a better understanding of the molecular signature changes during the whitening process of iBAT by transcriptomic analysis of rabbit
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Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery Adipocyte (IF 3.3) Pub Date : 2022-08-29 Shuai Chen, Yicheng Jiang, Xiaoyang Qi, Peng Song, Liming Tang, Hanyang Liu
ABSTRACT Bariatric surgery (BS) is a dependable method for managing obesity and metabolic diseases, however, the regulatory processes of lipid metabolism are still not well elucidated. Differentially expressed genes (DEGs) were analysed through three transcriptomic datasets of GSE29409, GSE59034 and GSE72158 from the GEO database regarding subcutaneous adipose tissue (SAT) after BS, and 37 DEGs were
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Insulin prevents fatty acid induced increase of adipocyte size Adipocyte (IF 3.3) Pub Date : 2022-08-22 Emmanuelle Berger, Alain Géloën
ABSTRACT Metabolic disorders related to obesity are largely dependent on adipose tissue hypertrophy, which involves adipocyte hypertrophy and increased adipogenesis. Adiposize is regulated by lipid accumulation as a result of increased lipogenesis (mainly lipid uptake in mature adipocytes) and reduced lipolysis. Using realtime 2D cell culture analyses of lipid uptake, we show (1) that high glucose
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The effect of trimethylamine N-oxide on the metabolism of visceral white adipose tissue in spontaneously hypertensive rat Adipocyte (IF 3.3) Pub Date : 2022-08-17 Guo-Dong He, Xiao-Cong Liu, Xing-Hua Hou, Ying-Qing Feng
ABSTRACT Strong links have been reported among trimethylamine N-oxide (TMAO), visceral white adipose tissue (vWAT), and cardiometabolic diseases. However, the effects of TMAO on vWAT in hypertension remained incompletely explored. The impact of a chronic 22-week-long treatment with 1 g/L TMAO on vWAT, and its transcriptional and metabolic changes in spontaneously hypertensive rats (SHRs) were evaluated
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ESR2 expression in subcutaneous adipose tissue is related to body fat distribution in women, and knockdown impairs preadipocyte differentiation Adipocyte (IF 3.3) Pub Date : 2022-08-17 Fozia Ahmed, Susanne Hetty, Milica Vranic, Giovanni Fanni, Joel Kullberg, Maria João Pereira, Jan W Eriksson
ABSTRACT Oestrogen receptor 2 (ESR2) expression has been shown to be higher in subcutaneous adipose tissue (SAT) from postmenopausal compared to premenopausal women. The functional significance of altered ESR2 expression is not fully known. This study investigates the role of ESR2 for adipose tissue lipid and glucose metabolism. SAT biopsies were obtained from 44 female subjects with or without T2D
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Bcl-2 modified adipose-derived stem cells improve the retention of fat graft Adipocyte (IF 3.3) Pub Date : 2022-08-17 Ziwei Cui, Qian Tan
ABSTRACT In cell-assisted lipotransfer, adipose-derived stem cells play a crucial role in enhancing fat graft retention. In vitro, human adipose-derived stem cells were modified with Bcl-2 gene. In vivo, aspirated fat was mixed with the Bcl-2-modified adipose-derived stem cells and then transplanted subcutaneously into nude mice. The retention of fat graft was evaluated. The surviving Bcl-2-modified
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RNA-Seq and lipidomics reveal different adipogenic processes between bovine perirenal and intramuscular adipocytes Adipocyte (IF 3.3) Pub Date : 2022-08-08 Xiaoyu Wang, Chengcheng Liang, Anning Li, Gong Cheng, Feng Long, Rajwali Khan, Jianfang Wang, Yu Zhang, Sen Wu, Yujuan Wang, Ju Qiu, Chugang Mei, Wucai Yang, Linsen Zan
ABSTRACT Adipogenesis involves complex interactions between transcription and metabolic signalling. Exploration of the developmental characteristics of intramuscular adipocyte will provide targets for enhancing beef cattle marbling without increasing obesity. Few reports have compared bovine perirenal and intramuscular adipocyte transcriptomes using the combined analysis of transcriptomes and lipid
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Mitochondrial transformation occurs in cultured adipocytes, but fails to increase adipose tissue metabolic activity in mice in vivo Adipocyte (IF 3.3) Pub Date : 2022-08-08 Lucia Balazova, Natalia Palesova, Miroslav Balaz
ABSTRACT A large number of studies in recent years have aimed to devise novel therapeutic strategies to increase adipose tissue metabolic activity and fight the global obesity epidemics. Growing evidence suggests that cells are able to accept isolated mitochondria by a simple coincubation in a process known as mitochondrial transformation. Therefore, we aimed to test whether mitochondrial transformation
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MAPKs/AP-1, not NF-κB, is responsible for MCP-1 production in TNF-α-activated adipocytes Adipocyte (IF 3.3) Pub Date : 2022-08-08 Xiaoyu Zhang, Zhuangzhuang Liu, Wenjing Li, Yuan Kang, Zhenlu Xu, Ximeng Li, Yuan Gao, Yun Qi
ABSTRACT Obesity is associated with the infiltration of monocytes/macrophages into adipose tissue in which MCP-1 plays a crucial role. But the regulatory mechanism of MCP-1 expression in adipocytes is not well defined. Our results demonstrated that TNF-α induced abundant MCP-1 production in adipocytes, including 3T3-L1 pre- (≈ 9 to 18-fold), mature adipocytes (≈ 4 to 6-fold), and primary adipocytes(<
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Cancer cell-derived exosomal miR-425-3p induces white adipocyte atrophy Adipocyte (IF 3.3) Pub Date : 2022-08-08 Anwen Liu, Wenxia Pan, Shutong Zhuang, Yuanzhi Tang, Haitao Zhang
ABSTRACT White adipose tissue wasting plays a critical role in the development and progression of cancer cachexia. However, the mechanism behind the loss of adipose tissue remains ill-defined. In this study, we found that cancer cell-derived exosomes highly expressed miR-425-3p. Administration of cancer cell-derived exosomes significantly inhibited proliferation and differentiation of human preadipocytes-viscereal
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PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes Adipocyte (IF 3.3) Pub Date : 2022-08-02 Fan Hu, Chang Li, Yafen Ye, Xuhong Lu, Miriayi Alimujiang, Ningning Bai, Jingjing Sun, Xiaojing Ma, Xiaohua Li, Ying Yang
ABSTRACT PARP12 is a member of poly-ADP-ribosyl polymerase (PARPs), which has been characterized for its antiviral function. Yet its physiological implication in adipocytes remains unknown. Here, we report a central function of PARP12 in thermogenic adipocytes. We show that PARP12 is highly expressed in brown adipose tissue and is mainly localized to the mitochondria. Knockdown of PARP12 in vitro reduced
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Brown adipose tissue influences adiponectin and thyroid hormone changes during Graves’ disease therapy Adipocyte (IF 3.3) Pub Date : 2022-07-27 Wei-En Ho, Lijuan Sun, Hui Jen Goh, Mya Thway Tint, Lei Sun, Melvin Khee Shing Leow
ABSTRACT Thyroid hormones (TH), adiponectin and brown adipose tissue (BAT) are regulators of energy homoeostasis. Influence of BAT activity on the relationship between TH and adiponectin remains unexplored. The aim of the study was to identify the relationship between TH and adiponectin and to clarify the impact of active BAT on the metabolic effects of adiponectin before and after the correction of
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Identification of key genes and their association with immune infiltration in adipose tissue of obese patients: a bioinformatic analysis Adipocyte (IF 3.3) Pub Date : 2022-07-27 Jie Wen, Liwen Wang
ABSTRACT Immune cell-mediated adipose tissue (AT) inflammation contributes to obesity-related metabolic disorders, but the precise underlying mechanisms remain largely elusive. In this study, we used the R software to screen key differentially expressed genes (DEGs) in AT from lean and obese individuals and conducted function enrichment analysis. We then analysed their PPI network by using the STRING
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Towards better models for studying human adipocytes in vitro Adipocyte (IF 3.3) Pub Date : 2022-07-27 Fabiana Baganha, Ruby Schipper, Carolina E. Hagberg
ABSTRACT With obesity and its comorbidities continuing to rise, we urgently need to improve our understanding of what mechanisms trigger the white adipose tissue to become dysfunctional in response to over-feeding. The recent invent of 3D culturing models has produced several noteworthy protocols for differentiating unilocular adipocytes in vitro, promising to revolutionize the obesity research field
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Pancreatic cancer cell exosomes induce lipidomics changes in adipocytes Adipocyte (IF 3.3) Pub Date : 2022-06-23 Shihua Wang, Meiqian Xu, Xian Xiao, Liping Wang, Zhao Sun, Mei Guan, Robert Chunhua Zhao
ABSTRACT Increasing evidence has demonstrated the important roles of exosomes during pancreatic cancer development. However, the effects of pancreatic cancer exosomes (PC-exos) on adipocytes remain largely unknown. Here, we used mass-spectrometry-based lipidomics to identify lipids that were changed in adipocytes after exposure to PC-exos, and we found that triglyceride (TG) reduction was the most
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Metformin and vitamin D modulate adipose-derived stem cell differentiation towards the beige phenotype Adipocyte (IF 3.3) Pub Date : 2022-06-23 Sara Cruciani, Giuseppe Garroni, Renzo Pala, Donatella Coradduzza, Maria Laura Cossu, Giorgio Carlo Ginesu, Giampiero Capobianco, Salvatore Dessole, Carlo Ventura, Margherita Maioli
ABSTRACT Adipose-derived stem cells (ADSCs) represent an ideal stem cell population for regenerative medicine. ADSC adipogenic differentiation is controlled by the activation of a specific transcriptional program, including epigenetic factors and key adipogenic genes. Under certain conditioned media, ADSCs can differentiate into several phenotypes. We previously demonstrated that bioactive molecules
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Cixutumumab reveals a critical role for IGF-1 in adipose and hepatic tissue remodelling during the development of diet-induced obesity Adipocyte (IF 3.3) Pub Date : 2022-06-23 Helen Imrie, Hema Viswambharan, Natalie J Haywood, Katherine I Bridge, Nadira Y Yuldasheva, Stacey Galloway, Katie J Simmons, Richard M Cubbon, Piruthivi Sukumar, Nicole T Watt, Laeticia Lichtenstein, Judy I Wyatt, Hiromi Kudo, Robert Goldin, Baptiste Rode, Stephen B Wheatcroft, Mark T Kearney
ABSTRACT High fat diet (HFD)-induced obesity leads to perturbation in the storage function of white adipose tissue (WAT) resulting in deposition of lipids in tissues ill-equipped to deal with this challenge. The role of insulin like growth factor-1 (IGF-1) in the systemic and organ-specific responses to HFD is unclear. Using cixutumumab, a monoclonal antibody that internalizes and degrades cell surface
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Identification of FOXM1 and CXCR4 as key genes in breast cancer prevention and prognosis after intermittent energy restriction through bioinformatics and functional analyses Adipocyte (IF 3.3) Pub Date : 2022-05-19 Lusha Li, Liangli Chen, Li Yu, Junlu Zhang, Liying Chen
ABSTRACT We explored potential biomarkers and molecular mechanisms regarding breast cancer (BC) risk reduction after intermittent energy restriction (IER) and further explored the association between IER and BC prognosis. We identified differentially expressed genes (DEGs) in breast tissues before and after IER by analyzing the expression profile from GEO. Then, enrichment analysis was used to identify
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Identifying sex differences in predictors of epicardial fat cell morphology Adipocyte (IF 3.3) Pub Date : 2022-05-19 Helen M. M. Waddell, Matthew K. Moore, Morgan A. Herbert-Olsen, Martin K. Stiles, Rexson D. Tse, Sean Coffey, Regis R. Lamberts, Hamish M. Aitken-Buck
ABSTRACT Predictors of overall epicardial adipose tissue deposition have been found to vary between males and females. Whether similar sex differences exist in epicardial fat cell morphology is currently unknown. This study aimed to determine whether epicardial fat cell size is associated with different clinical measurements in males and females. Fat cell sizes were measured from epicardial, paracardial