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Ovarian lavage / artificial insemination method as an alternative approach for controlled reproduction of the northern pike (Esox lucius) Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-03-12 Erfan Akbari Nargesi, Danial Gorouhi
This study aimed to compare the effectiveness of the ovarian lavage / artificial insemination method with the traditional hormonal administration and fertilization methods over the artificial reproduction of the northern pike (). For this purpose, groups of five females were treated as follows: intraperitoneal injection of saline (C1); ovarian lavage with saline (C2); intraperitoneal injection of carp
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Maternal recognition of pregnancy in the pig: A servomechanism involving sex steroids, cytokines and prostaglandins Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-03-12 Rodney D. Geisert, Fuller W. Bazer, Caroline G. Lucas, Caroline A. Pfeiffer, Ashley E. Meyer, Riley Sullivan, Destiny N. Johns, Mariana Sponchiado, Randall S. Prather
Maternal recognition of pregnancy (MRP) is a term utilized in mammals to describe pathways in which the conceptus alters the endometrial environment to prevent regression of corpora lutea to ensure continued production of progesterone (P4) required for establishment and maintenance of pregnancy. For nearly 40 years after publication of the endocrine/exocrine theory, conceptus estrogen (E2) was considered
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The opioid peptide leucine enkephalin modulates hypothalamic-hypophysial axis in the cichlid fish Oreochromis mossambicus Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-03-08 Deepak Shinde, Shilpa K. Bhat, C.B. Ganesh
In vertebrates, opioid peptides are thought to be involved in the regulation of reproduction; however, the significance of enkephalins in testicular function remains unclear. We examined the influence of δ-opioid receptor agonist leucine enkephalin (L-ENK) on the hypophysial-testicular axis of the cichlid fish . Treatment with a low dose of L-ENK (60µg) caused a significant increase in the numbers
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In vitro characteristics of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and TLR7/8 ligand R848 Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-03-02 Stanimir Yotov, Desislava Abadjieva, Anatoli Atanasov, Boyana Ivanova, Paulina Taushanova, Ivan Fasulkov, Elena Kistanova
The quality of the separated fractions in sex-sorted semen is very important for the success of the artificial insemination. This study aimed to evaluate some in vitro characteristics (DNA quantity, kinematic parameters and enzymes activity) of X- and Y-bearing ram spermatozoa sorted by bovine serum albumin (BSA) column and toll-like receptors (TLR)7/8 ligand R848. The ejaculates from six rams were
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Simple method for isolation and culture of primary buffalo (Bubalus bubalis) endometrial epithelial cells (pBuEECs) and its characterization using high throughput proteomics approach Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-29 Shradha Jamwal, Nikunj Tyagi, Jaideep Kumar, Jai Kumar Kaushik, Sudarshan Kumar, Ashok Kumar Mohanty
Early embryonic mortality resulting from insufficient interaction between the embryo and the uterus leads to the failure of pregnancy in livestock animals. Thus, it is imperative to comprehend the multifaceted process of implantation at molecular levels, which requires synchronized feto-maternal interaction. The models serve as valuable tools to investigate the specific stages of implantation. The
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Integrated omics analysis reveals a correlation between gut microbiota and egg production in captive African penguins (Spheniscus demersus) Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-27 Jingle Jiang, Di Hu, Enle Pei
The egg production of captive African penguins differs considerably between individuals. An understanding of the physiological differences in African penguins with relatively greater and lesser egg production is meaningful for the captive breeding program of this endangered species. The objective of this study was to investigate differential microbial composition and metabolites in captive African
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Sperm traits and seminal plasma proteome of locally adapted hairy rams subjected to intermittent scrotal insulation Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-24 Aderson M Viana Neto, Denise D. Guerreiro, Jorge A.M. Martins, Fábio R. Vasconcelos, Révila B.F. Melo, Ana Luiza M.C. S Velho, Marta Neila-Montero, Rafael Montes-Garrido, Celso S. Nagano, Airton A. Araújo, Arlindo A. Moura
The present study evaluated the effects of heat stress on reproductive parameters of hairy rams. Six animals were subjected to scrotal insulation during four consecutive nights (6 PM – 6 AM). Day (D) 0 was the first day of insulation. Scrotal circumference increased from 30.5 ± 0.3cm (at pre-insulation) to 31.8 ± 0.4cm on D4, decreased 3.9cm on D28, returning to 30.6 ± 0.6cm on D57. Sperm concentration
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Genetic parameters for bull effects on in vitro embryo production (IVP) and relationship between semen quality traits and IVP performance Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-23 G. Gebreyesus, J.B. Secher, M.S. Lund, K. Kupisiewicz, M. Ivask, T. Hallap, P. Pärn, G. Su
production of embryos (IVP) is increasingly applied in dairy cattle breeding and promises widespread use of females of superior genetic merits. One of the current challenges with implementation of IVP is the variability in blastocyst rates. Several factors contribute to these variabilities, among which is known to be the bull used for oocytes fertilization. The extent of genetic control of bulls’ effect
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An updated review on the application of proteomics to explore sperm cryoinjury mechanisms in livestock animals Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-21 Allai Larbi, Chunyan Li, Guobo Quan
This comprehensive review critically examines the application of proteomics in understanding sperm cryoinjury mechanisms in livestock animals, in the context of the widespread use of semen cryopreservation for genetic conservation. Despite its global adoption, cryopreservation often detrimentally affects sperm quality and fertility due to cryoinjuries. These injuries primarily arise from ice crystal
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Split weaning improves pregnancy rate and embryo survival in sows mated in lactation Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-21 Alice C Weaver, Karen L Kind, Paul J Herde, William H E J van Wettere
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Reproductive tract morphology and symmetry of farmed common eland (Tragelaphus oryx) bulls, and their relationship with secondary sexual traits and social rank Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-17 Jerico Consolacion, Francisco Ceacero, Abubakar Sadiq Musa, Veit Ny, Radim Kotrba, Josef Illek, Miša Škorič, Tersia Needham
Common eland bulls are important game ranching animals in southern Africa, for tourism, breeding/live sales, trophy hunting, and game meat production. Interest has grown in their production potential, intensifying animal husbandry and breeding approaches. However, little scientific information is available regarding the intensive management of this species, including information regarding scientifically
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Effect of chemical activators after intracytoplasmic sperm injection (ICSI) on embryo development in alpacas Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-14 Dionet Keny Bellido Quispe, Fidel Rodolfo Mujica Lengua, Mijaíl Contreras Huamani, J Manuel Palomino
Low motility and low sperm concentration are characteristics of alpaca semen. Thus, the intracytoplasmic sperm injection (ICSI) technique represents an alternative to improve the reproductive capacity of the male. However, the effect of post-ICSI activation in alpaca is not yet known. The aim of the present study was to compare the effect of chemical activators on alpaca embryo development after ICSI
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Effects of maternal nutrient restriction during gestation on bovine serum microRNA abundance Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-13 K.J. McCarty, A.N. DeCarlo, R.E. Ricks, S.L. Pratt, N.M. Long
The objective was to determine the effects of maternal nutrient restriction during gestation on serum microRNA (miRNA) abundance in cattle. Primiparous Angus-cross cows (=22) were fed either control (CON; to gain 1 Kg/week) or nutrient restricted (NR; 0.55% NE) diets based on National Research Council requirements. On day 30 of gestation, cows were blocked by body condition and randomly assigned to
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Effect of split weaning on follicle development and oocyte quality in multiparous sows Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-13 Alice C. Weaver, Karen L. Kind, Jennifer M. Kelly, Paul Herde, William HE J. van Wettere
Increasing piglet weaning age while maintaining the reproductive efficiency of the breeding herd depends on being able to stimulate sows to ovulate during lactation without reducing subsequent pregnancy rates and litter sizes. Embryo survival is affected by the quality of the oocytes shed at ovulation, and oocyte quality is profoundly impacted by the follicular environment in which the oocyte matures
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Transferrin maintains the motility rate, ATP content, and DNA integrity of common carp spermatozoa during short-term storage Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-13 Anna Shaliutina-Loginova, Dmitry S. Loginov
Short-term sperm storage is a straightforward and cost-effective method of managing logistics in large scale fish hatchery operations but may result in decline in sperm quality. For effective artificial reproduction of fish, use of an appropriate additive to optimize sperm storage conditions is essential. In this study, it was investigated the effect of purified seminal plasma transferrin (Tf) at 10μg/ml
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Maternal prepartum supplementation of protein and energy and body condition score modulated the performance of Bos indicus-influenced cow-calf pairs Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-12 P. Moriel, M. Vedovatto, V. Izquierdo, E.A. Palmer, J.M.B. Vendramini
Retrospective analyses were performed on a dataset of 1,188 fall-calving, Brangus cow-calf pairs. Analyses 1 sorted cows according to their initial body condition score ( vs. ) and whether they received () or not () prepartum supplementation of protein and energy. Analyses 2 sorted cows according to their calving BCS ( or ) and BCS change from calving until the start of the breeding season (lost, maintained
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Prolonged cold-preservation of domestic cat ovarian tissue is improved by extracellular solution but impaired by the fragmentation of ovary Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-11 Olga Rodak, Manuel David Peris-Diaz, Piotr Dzięgiel, Aleksandra Piotrowska, Agnieszka Partyka, Wojciech Niżański
For domestic cats ovaries, recommended cold-storage limit is 24h in Phosphate Buffered Saline (PBS) or Dulbecco`s PBS (DPBS). Here, we attempted to verify wheatear cat ovaries may benefit from more complex solutions during prolonged cold-storage (>24h).
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Effect of mastitis during early-stage pregnancy on the immunity levels and pregnancy function of goats Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-09 Zi-Long Liang, Nozomi Kodama, Naoki Isobe
In this study, we investigated the effects of mammary inflammation induced by lipopolysaccharide (LPS) and (SA) infusions on pregnancy function during early pregnancy in goats. In Experiment 1, pregnant goats were subjected to an intramammary LPS infusion for 1 week from Days 60–66 after natural mating ( = 5), and in Experiment 2, they received intramammary infusions of either saline, LPS, or SA for
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L-Proline: A Promising Tool for Boosting Cryotolerance and Fertilizing Ability of Cryopreserved Sperm in Animals Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-09 Sameh A. Abdelnour, Wael A. Khalil, Norhan E. Khalifa, Fatma Mohamed Ameen Khalil, Mahmoud A.E. Hassan
Sperm cryopreservation technology significantly contributes to the safeguarding of genetic resources, particularly for endangered species, and supports the use of artificial insemination in domestic animals. Therefore, cryopreservation can negatively affect sperm health and function leading to reduce the freezing ability and fertility potential. Therefore, it is essential to prioritize the improvement
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ProAKAP4 as a motility long-lasting marker in Catalan donkey spermatozoa Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-07 Marta Dordas-Perpinyà, Nicolas Sergeant, Iván Yánez-Ortiz, Vincent Mevel, Jaime Catalán, Jean-François Bruyas, Lamia Briand-Amirat, Jordi Miró
ProAKAP4 is identified within the flagellum of spermatozoa in various mammalian species, serving as a structural protein associated with motility parameters. This investigation focuses on the presence of proAKAP4 in donkey sperm, elucidating its localization, molecular characteristics, and its correlation with motility descriptors and mitochondrial membrane potential. Twelve ejaculates from Catalan
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The importance of developmental programming in the dairy industry Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-05 Maya Meesters, Mieke Van Eetvelde, Barbara Beci, Geert Opsomer
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Effect of anti-adhesive supplement doses on sperm motility characteristics in the European whitefish (Coregonus lavaretus) Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-28 Beata Irena Cejko, Dorota Fopp-Bayat, Roman Kujawa
Sperm adhering to glass slides is one of the main problems during fish sperm motility analyses with CASA systems. To mitigate this, albumin is the supplement added most frequently to activating solutions. However, there is no data on the use of supplements other than albumin (in various concentrations) in analyses of European whitefish (Coregonus lavaretus) sperm motility. This issue was investigated
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Oocyte competence and gene expression in parthenogenetic produced embryos from repeat breeder and normally fertile buffaloes (Bubalus bubalis) raised in sub-humid tropical climate Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-02-02 Satish Kumar, Maiana Silva Chaves, Anna Clara Accioly Ferreira, Ana Flavia Bezerra da Silva, Leda Maria Costa Pereira, William Gomes Vale, Sebastião Tavares Rolim Filho, Yeda Fumie Watanabe, Luciana Magalhães Melo, Vicente José de Figueirêdo Freitas
The reproductive management of the buffalo species still faces several unresolved problems, which directly affect the productivity of the herd, one of them being the presence of repeat breeder females. Given this scenario, this study aimed to verify the developmental competence of oocytes obtained from repeat breeder females and submitted to parthenogenetic activation. In addition, embryo gene expression
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Refrigerated storage and cryopreservation of hormonally induced sperm in the threatened frog, Litoria aurea Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-29 Rose Upton, Natalie E. Calatayud, Simon Clulow, Darcie Brett, Alana L. Burton, Kim Colyvas, Michael Mahony, John Clulow
As sperm cryopreservation and other assisted reproductive technologies (ARTs) advance in common amphibian species, focus on applying non-lethal sperm collection methods to the conservation and genetic management of threatened species is imperative. The goal of this study was to examine the application of logistically practical ART protocols in a threatened frog (Litoria aurea). First, we tested the
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Altered expression of angiogenic factors in dominant preovulatory follicles of dairy cattle treated with ACTH Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-24 Fátima Belén Gasser, Antonela Florencia Stassi, Melisa María del Lujan Velázquez, Lucas Etchevers, Ayelén Noelia Amweg, Natalia Raquel Salvetti, Hugo Héctor Ortega, María Eugenia Baravalle
Studies in cows have reported that ovulation, steroidogenesis and angiogenesis are affected by stress and consequently fertility decreases. The purpose of this study was to evaluate the effects of ACTH administration during the preovulatory period on the expression of growth factors (CD-31, PDGF-A, PDGF-B, VEGFA-164, VEGFA-164b, VEGF-R1 and VEGF-R2) associated with the angiogenic process by immunohistochemistry
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Enhancement potential of Moringa Oleifera leaves extract on buffalo bull cryopreserved semen quality and fertilization capacity Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-26 Dina Mahdy Shokry, Magdy Ramadan Badr, Abdel-Aziz Sakr, Ahmed Mohamed Elmesiry, Mohamed Mahmoud Assy, Zaher Rawash, Mabrouk Attia Abd Eldaim
The aim of the present study is to evaluate the effect of Moringa Oleifera leave extract (MOLE) on buffalo bull cryopreserved semen quality and fertility. Sixty ejaculates were collected from ten fertile buffalo bulls on a weekly basis for 6 weeks (n = 10 bulls & n = 60) then semen samples were pooled and divided into five groups. The semen of the control group was without additives. The semen of other
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Effects of vibrations during boar semen transport: Low-temperature transport as a new management tool Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-17 Britta Hensel, Sophie Henneberg, Anja Riesenbeck, Markus Jung, Martin Schulze
Transport-related vibrations (TV) compromise the quality of conventionally stored (17 °C) boar semen, but knowledge about TV effects after 5 °C transport is insufficient. This study evaluates the effects of TV after novel 5 °C transport compared to a 17 °C control. Ejaculates of 18 fertile Piétrain boars, diluted in a split sample procedure using Androstar Premium® (AP, 5 °C storage) or Beltsville
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In vivo exposure to high temperature compromises quality of the sperm in Colossoma macropomum Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-18 Jonatas da Silva Castro, Francisco Bruno Pereira Santos, Jaqueline Custódio da Costa, Waldir Heinrichs-Caldas, Adalberto Luis Val
Increases in temperature can affect the reproduction of fish by decreasing the quality of gametes for fertilization. Therefore, this study aimed to evaluate the in vivo effect of temperature on the production and sperm quality of Colossoma macropomum, which is an economically relevant species for Brazil, and other countries. Broodstock were exposed for 10 days at temperatures of 29 °C (n = 4) and 35 °C
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Artificial reproduction of wild and pond-cultured common tench (Tinca tinca) under controlled conditions Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-10 Dariusz Kucharczyk, Wojciech Fopp, Pawe Wojtyszek, Joanna Nowosad, Daria J. Kucharczyk, Jakub Drężek, Agnieszka Kołosińska, Nazira Omirzhanova, Grzegorz J. Dietrich
The common tench is an important thermophilic freshwater fish used for aquaculture diversification. This species is farmed in ponds as well as caught in the wild in many European and China. Artificial reproduction under controlled conditions is one of the most important part of modern aquaculture. In this study, two experiments were conducted in which the influence of hormonal agents (Ovopel and Ovaprim
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Influence of photoperiod and exogenous melatonin on testis morpho-physiology of sexually mature guinea fowl (Numida meleagris) Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-10 Innocent J. Gosomji, Umar M. Bello, Tavershima Dzenda, Abdullahi Baso, Augustine Arukwe, Tom A. Aire
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Steroidogenic activity and morphological characterization of prenatal testes and epididymis of guinea pig (Cavia porcellus) Anim. Reprod. Sci. (IF 2.2) Pub Date : 2024-01-02 G.A.A. Lemos, A.C. Santos, D.C.C. Brito, M.A.S. Novaes, A.C. Assis Neto
The present study aims to establish the morphological, morphometric, and immunostaining patterns of the steroidogenic enzymes 17β-HSD and 5α-reductase and androgen receptors (AR) during the prenatal development of the male gonad and epididymis of Cavia porcellus. Fetuses at 22, 25, 30, 40, 45, 50, and 60 days of gestation (DG) were used. Specimens were dissected and subjected to macroscopic, histological
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Morphological evidence for the physiological nature of follicular atresia in veiled chameleons (Chamaeleo calyptratus) Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-31 Eva Dervas, Pia Cigler, Jean-Michel Hatt, Maya S. Kummrow
Follicular atresia (FA) has been assumed to serve different functions in reptiles, e.g. helping to develop hierarchies, limiting clutch size, and regression of ovarian structures. Reproductive output is dependent on a balance between ovulations and FA. Excessive rates of FA may not only be detrimental for the survival of a population, but have also been associated with pathological conditions. In order
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Estimation of gestational age in buffaloes (Bubalus bubalis) by transabdominal and transrectal ultrasonography Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-29 Ebubekir Yazıcı, Oktay Yılmaz, Erhan Özenç, Mehmet Uçar, Hacı Ahmet Çeli̇k
The aims of the presented study were (1) to examine the relationship between foetal measurements and gestational age (GA), (2) to generate GA formulas, and (3) to investigate the estimation of GA by transabdominal ultrasonography in buffaloes. Thirteen pregnant buffaloes were used in the study. Transrectal ultrasonography was performed between Day (D) 28 and 112 of gestational age, whereas transabdominal
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Epidermal growth factor: Expression in goat endometrial epithelia during early pregnancy and regulation by interferon tau and FOXO1 Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-21 Bo Wang, Meng-Die Li, Hai-Yue Xu, Xin-Cheng Zhang, Li-Ge Bu, Ting-Yue Li, Ya Sun, Hua Ni
In ruminants, establishment and maintenance of pregnancy depends upon a well-coordinated interaction between the conceptus and the maternal endometrium. Epidermal growth factor (EGF) is important for embryo implantation and pregnancy establishment. However, the regulatory mechanisms of EGF expression remain unclear. FOXO1, a member of the Forkhead box O (FOXO) subfamily of transcription factors, is
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Development and growth of bovine calves demi-embryos Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-21 Amada E. Ynsaurralde-Rivolta, Andres Rogberg-Muñoz, Sebastian Lopez-Valiente, Sebastian Maresca, Alejandro Rodriguez, Carlos Munar, Sebastian Munilla-Leguizamón, Franco A. Dellavalle, Daniel Salamone
The aim of this study was to investigate the growth and development of animals produced from demi-embryos and compare them with whole embryos from fetus to adult life. To achieve this, calves produced from fresh demi-embryos and whole embryos were individually transferred and monitored from 60 days of pregnancy until slaughter at 550 days. Ultrasound scans were conducted on fetuses at 60 and 90 days
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Evaluation of a long-acting recombinant bovine FSH for multiple ovulation and embryo transfer in dromedary camels Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-15 Mohamed El-Shazly, Nabil Mansour, Aly Karen, Mahmoud Salama, Ibrar Hijazi, Mahmoud El-Ghazaly, Kasem Sheply, Scott Jaques
The present study was conducted to test a new super-agonist recombinant bovine FSH (rbFSH) to induce superovulation (SOV) in dromedary camels. In experiment I, a single IM injection of 40, 60, 80, 100, or 120 µg rbFSH was administered (4 donors per group) to determine the effective dose resulting in acceptable multiple ovulation and embryo yield. Administration of 40 µg was ineffective, while 100 and
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Luteal tissue characteristics of Morada Nova ewes with hCG application 7.5 days after the end of estrus synchronization protocol in the breeding season Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-14 Joedson Dantas Gonçalves, Gabriel Brun Vergani, Juliana Nascimento Duarte Rodrigues, Jenniffer Hauschildt Dias, Verônica Schinaider do Amaral Pereira, Alexandre Rossetto Garcia, Sergio Novita Esteves, Jeferson Ferreira da Fonseca, Maria Emilia Franco Oliveira
Results with the use of hCG after synchronization protocol are still inconsistent, which may vary according to breed, season, day of application and dose of the drug used. The aim of the present study was to evaluate the functionality of luteal tissue and ovarian perfusion after hCG treatment during early luteal phase. Estrus-synchronized ewes were randomly assigned to receive i.m. injection of 300
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Effect of follicular wave synchronization using a progesterone-releasing intravaginal device-based protocol on in vitro embryo production in Bos taurus cows subjected to 14-day intervals ovum pick-up Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-13 Juan Patricio Anchordoquy, Marianela Balbi, Nicolás A. Farnetano, Mauricio J. Giuliodori, Noelia Nikoloff, Ana C. Carranza-Martín, Cecilia C. Furnus, Juan Mateo Anchordoquy
Follicular wave synchronization (FWS) before ovum pick-up (OPU) is one of the strategies used to improve the efficiency of in vitro embryo production (IVP). This study aimed to evaluate the effect of FWS on the total follicular number, cumulus-oocyte complex (COC) recovery, and in vitro embryo development in Angus cows (n = 33) subjected to OPU with 14-day intersession intervals. Additionally, it was
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Collection of epididymal semen in the tomcat (Felix catus) by stereomicroscope-aided retrograde flushing (SARF) improves sample quality Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-12 Davide Monaco, Alessandra Rota, Alice Carbonari, Edoardo Lillo, Giovanni Michele Lacalandra, Annalisa Rizzo
In small and laboratory animals epididymal spermatozoa can be collected only by the mincing technique however, blood and cell debris contamination may adversely affect the quality of the samples. This study aimed at performing the retrograde flushing technique, for the first time, in the tomcat. In the first experiment attempts to insert a blunted needle into tomcats’ ductus deferentes were made and
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Morphological and transcriptomic analyses of embryonic development of red-eared slider Trachemys scripta elegans Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-12-07 Jing Lin, Miaomiao Zhang, Fangbin Liang, Yunfang Ni, Jiani Zhang, Haitao Shi, Meiling Hong, Li Ding
Embryology provides an understanding of individual’s origin and developmental patterns. Turtles are among the oldest living reptiles and have unique body structure. However, the morphogenesis and mechanisms of turtles are not fully understood. In this study, we focused on the embryonic development of red-eared slider (Trachemys scripta elegans) which widely distributes in the world. At an incubation
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L-cysteine improves boar semen motility at 5 ºC but does not affect the oxidative status Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-29 Guilherme dos Santos, Mayara de Souza Costa Tamanini, Leonardo Abreu Leal, Lucas Michel Wolf, Thaís Spohr Christ, Yasmin Vendruscolo Piton, Marcelo Dutra Arbo, Mari Lourdes Bernardi, Rafael da Rosa Ulguim, Fernando Pandolfo Bortolozzo, Ana Paula Gonçalves Mellagi
Hypothermic storage has been proposed as a method to reduce bacterial loads and promoting prudent use of antibiotics. Reducing temperature, however, can lead to cold shock damage and oxidative stress in boar semen. This study verified the effect of L-cysteine on the quality of semen stored at 5 °C for 120 h. Twenty-one normospermic ejaculates were diluted in Beltsville Thawing Solution into five treatments:
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Additional glial cell line-derived neurotrophic factor in vitro promotes the proliferation of undifferentiated spermatogonia from sterile cattleyak Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-30 Peng Zhang, Kemin Jing, Yuan Tian, Yuqian Li, Zhixin Chai, Xin Cai
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Mito-Q supplementation of in vitro maturation or in vitro culture medium improves maturation of buffalo oocytes and developmental competence of cloned embryos by reducing ROS production Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-25 Maninder Sharma, Meeti Punetha, Sheetal Saini, Suman Chaudhary, Sujata Jinagal, Swati Thakur, Pradeep Kumar, Rajesh Kumar, R.K. Sharma, P.S. Yadav, Dharmendra Kumar
Mito-Q is a well-known mitochondria-specific superoxide scavenger. To our knowledge, the effect of Mito-Q on buffalo oocyte maturation and developmental competency of cloned embryos has not been examined. To investigate the effects of Mito-Q on the in vitro maturation (IVM) of buffalo oocytes and the developmental competence of cloned embryos, different concentration of Mito-Q were supplemented with
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Selection of spermatozoa with high motility and quality from bovine frozen-thawed semen using the centrifuge-free device Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-29 Suong T. Nguyen, Ayane Edo, Megumi Nagahara, Takeshige Otoi, Masayasu Taniguchi, Mitsuhiro Takagi
This study aimed to assess the potential of the centrifuge-free commercial device (MIGLIS®) in selecting functional frozen-thawed bovine sperm by migration-sedimentation, its effect on embryo development, and compare the potential with that of centrifugation-based techniques, including washing and Percoll density gradient centrifugation (DGC). In experiment 1, different dilutions (1.5×, 2×, and 3×)
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A comparison of the biological properties of European red deer (Cervus elaphus elaphus) spermatozoa stored in the epididymides and in a liquid state at 5 °C Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-29 Nicoletta M. Neuman, Anna Dziekońska, Aleksandra Orzołek, Przemysław Gilun
The biological properties of European red deer (Cervus elaphus elaphus) spermatozoa stored in the epididymides and in a liquid state were compared. Spermatozoa were collected from the epididymides harvested post-mortem. In the first variant, spermatozoa were diluted in two extenders (Bovidyl® and Salomon's), and were stored at 5 °C for up to 144 h. In the second variant, spermatozoa were stored in
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Cooling of alpaca spermatozoa using an extender with the addition of different percentages of seminal plasma Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-28 Mariana Lucía Bertuzzi, Edita Yola Torres, Manuel Guido Pérez Durand, Teodosio Huanca, Susana María Giuliano, María Ignacia Carretero
The objective of this study was to evaluate the effect of the addition of different percentages of seminal plasma (SP) during the cooling at 5 °C of alpaca spermatozoa from vas deferens. Fifteen pools of sperm from vas deferens were evaluated and then divided into four aliquots that were diluted to a final concentration of 30 × 106 sperm/ml with either: (1) Tris with 20% egg yolk (T-EY) (control, 0%
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The effect of dietary omega-3 fatty acid supplementation on fetal growth, piglet birth weight and plasma fatty acid concentrations, using docosahexaenoic acid in early gestation in sows Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-22 Thomas S. Bruun, Anja H. Madsen, Emilie R. Handberg, Jacob Dall, Søren K. Jensen, Esben Østrup, Anja V. Strathe
The objective of the study was to test the effect of the omega-3 fatty acid docosahexaenoic acid (DHA) on fetal and placental development as well as the birth weight of piglets. A total of 238 multiparous sows were allocated to either a control diet group or a DHA diet group with an omega-6 to omega-3 ratio of 9.8 and 2.4, respectively, from mating to day 43 of gestation. A blood sample was collected
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Localization and expression of phospholipase A2 and polyunsaturated fatty acid profile in the testis tissues of Hu sheep Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-23 Weili Liang, Yanhong Zhao, Guodong Quan, Rongyu Yao, Hua Chen, Xiuxiu Weng, Wanhong Li, Xiangpeng Yue, Fadi Li
The fatty acid content and the localization and expression of phospholipase A2 (PLA2) in the testis of Hu sheep were investigated. A total of 18 six-month-old Hu sheep were divided into small group (S, with left testis weight < 50 g), medium group (M, with left testis weight among 90–110 g), and large group (L, with left testis weight >160 g), which had six individuals each. The expression of PLA2
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Effect of vitamin E, D-limonene, and their combination on nulliparous rabbit reproductive performance Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-17 Anas A. Salem, Doaa A. Taha, Asmaa AE Nasr, Mohamed El-Sagheer, Wael Daghash, Raghda A. Taghian
Oxidative status is important in reproductive performance and using two natural antioxidants is more beneficial than one in nulliparous rabbits. The goal, effect of vitamin E (VitE), D‐limonene (DL), and VitE+DL on maternal LBW (MLBW), conception (CR), pregnancy (PR), and kindling rates (KR), gestation length (GL), total litter size at birth (TLSB) and weaning (TLSW), live kits at birth (LKB) and weaning
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Comparison of deep-litter bedding materials and analysis of semen traits in Piétrain boars: A randomized controlled field study Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-16 Anine Riedel, Laura Pieper, Matthias Lautner, Claus Leiding, Markus Jung, Martin Schulze
External factors can affect reproductive traits of breeding boars and especially the sensitive process of spermatogenesis. The aim of this study was to investigate probable influences of bedding materials (chipsy wood shavings (CWS), hemp straw (HS), linen straw (LS), spelt husks (SH), and regional wood shavings (RWS)) on semen traits of 40 randomly selected Piétrain boars (8 boars per group, age:
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The cryoprotective effect of Guar gum co-supplemented with ethylene glycol and glycerol in Simmental bull semen Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-07 Adel Saberivand, Raheleh Mehrabi, Seyedeh Zeinab Peighambarzadeh, Maryam Saberivand, Mahdiye Shirafkan, Tannaz Haghgouei, Fatemeh Sarvarzadeh, Peghah Safaei, Marzieh Saki, Koosha Javdaneh Siahpoush, Toran Alizadeh
Sperm cryopreservation often reduces sperm quality by forming of intra- and extracellular ice crystals. Various compounds widely used to counteract this effect. The guar gum was considered as an extracellular cryoprotective substance. The present study evaluated the impact of the co-supplementation of guar gum with ethylene glycol or glycerol in the cryopreservation of bull sperm. Four ejaculates from
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Effect of crocin supplementation in the extender on the quality of chilled canine semen Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-08 Alfonso Calabria, Chiara Del Prete, Ciarcia Roberto, Valentina Longobardi, Stefano Spada, Maria Teresa Alfano, Daniela De Felice, Bianca Gasparrini, Natascia Cocchia
The aim of the study was to evaluate the effects of crocin on canine sperm quality parameters during prolonged storage at 4 °C. Ejaculates from 10 dogs were diluted in a TRIS- egg yolk extender supplemented with 0 (control group), 0.5, 1, and 2 mM crocin and stored at 4 °C. Sperm membrane functional integrity, motility, and kinetics were assessed after 3 h, 24 h, 4 days and 7 days of storage. Based
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Determination of the timing of early gonadal differentiation in silver pomfret, Pampus argenteus Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-07 Chang Li, Yaya Li, Chunlai Qin, Changhang Yu, Jiabao Hu, Chunyang Guo, Yajun Wang
Silver pomfret is a species of global significance due to its high nutritional in fisheries sector. To accurately ascertain the timing of sex differentiation mechanism and mRNA level in this species, this study examined gonad morphology and patterns of gene expression related to sex differentiation in males and females from 51 to 180 days post hatch (dph), the temperature of water was maintained at
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Evaluation of the effect of melatonin implantation in rams and eCG dose in ewes synchronized by a CIDR-eCG protocol on reproductive performance of Lacaune sheep breed during non-breeding season Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-11-04 Navid Jahan Roshan, Massoud Talebkhan Garoussi, Vahid Akbarinejad
Sustainable production of milk is favorable in dairy sheep industry, which necessitates year-round reproduction of rams and ewes even during non-breeding season. Hence, protocols facilitating reproduction of rams and ewes during non-breeding season are of importance for this purpose. Therefore, the present study was conducted to investigate the effect of melatonin implantation in rams and administration
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An extreme model of fertility in sheep demonstrates the basis of controversies surrounding antral follicle count and circulating concentrations of anti-Müllerian hormone as predictors of fertility in ruminants Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-10-29 Robert A. Cushman, Hannah K. Yake, Alexandria P. Snider, Clay A. Lents, Thomas W. Murphy, Bradley A. Freking
Ovarian ultrasonography and measurement of circulating concentrations of anti-Müllerian hormone (AMH) have been used to estimate follicle number and predict fertility in mammalian females, but no study has evaluated follicle number and circulating concentrations of AMH in ewes known to differ in fertility. We tested the hypothesis that ewes that had failed to lamb (BARREN) in four consecutive annual
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Effects of different cryopreservation methods on canine isolated preantral follicles Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-10-20 Bence Somoskői, Lilla Bordás, Fusa Uno, Dóra Kispál, Linda Müller, Dóra Török, Sándor Cseh
The aim of the present study was to compare the survival and developmental rate of canine isolated preantral follicles (PAFs) after cryopreservation with different methods (closed vs open vitrification). Follicles were isolated from ovaries randomly divided into three groups: fresh control, OPS (open pulled straw) vitrified and cryotube (CT) vitrified. Post-thaw viability of follicles and oocytes was
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Reproductive biology of the totoaba (Totoaba macdonaldi), an endangered species in the Gulf of California Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-10-23 Carmen Rodríguez-Jaramillo, Fausto Valenzuela-Quiñonez, Eduardo F. Balart, Elena Palacios-Mechetnov, Lia Celina Méndez-Rodríguez, Martha J. Román-Rodríguez, Juan Antonio De-Anda-Montañez
The totoaba (Totoaba macdonaldi) is an emblematic species endemic to the Gulf of California, which has been protected since the mid-70 s by Mexican and international laws. For the present study, 153 totoaba individuals were collected from 2011 to 2014, on which gonad histology analyses were carried out. We identified six ovarian phases and eleven oocyte subphases in the gonad development of females;
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Identification of a circRNA-miRNA-mRNA network to explore the effects of circRNAs on Holstein bull testis after sexual maturity Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-10-21 Bahlibi Weldegebriall Sahlu, Huan Wang, Zhihui Hu, Nuo Heng, Jianfei Gong, Haoyu Wang, Huabin Zhu, Shanjiang Zhao
Spermatogenesis is an extremely sophisticated and complex process and is regulated not only by a large number of genes, but also by a large number of epigenetic factors. Although existing studies have demonstrated that circRNAs plays an important regulatory role in spermatogenesis, there is still insufficient information to properly understand the regulatory role and mechanism of circRNA action. We
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Effect of L-proline on sperm quality during cryopreservation of boar semen Anim. Reprod. Sci. (IF 2.2) Pub Date : 2023-10-09 Chuang Liu, Haoyi Feng, Jinyi Han, Hao Zhou, Liwei Yuan, Hongshuang Pan, Xiaohan Wang, Xuelei Han, Ruimin Qiao, Feng Yang, Xinjian Li
L-proline has been reported to be useful in semen cryopreservation. However, its use has rarely been reported in the freezing of boar semen. The objective of this study was to evaluate the effects of different concentrations of L-proline (0, 10, 30, 50, and 90 mM) on the quality of boar semen after freezing and thawing. Semen samples from boars (n = 6) were frozen using freezing extenders with added