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Rapid detection of vanB vancomycin-resistant enterococci by laboratory-developed PCR on enrichment broth Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-28 Anja Lindis Dahl, Martin Barfred Friis, Hanne Wiese Hallberg, Gitte Qvist Kristiansen, Barbara Juliane Holzknecht
Diagnostic accuracy of laboratory-developed PCR after overnight enrichment for the detection of vancomycin-resistant enterococci was evaluated on 537 rectal swabs. Defining Ct-values of 27-34 (40 samples, 7 % inconclusive), we found an excellent sensitivity of 98,3 % and specificity of 99,7 % for the remaining 497 samples.
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The quality of antimicrobial susceptibility test discs and implications for clinical outcomes Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-27 Stefan T Orszulik
Disc diffusion remains the main technique for evaluating antibiotic activity in clinical microbiology laboratories. However, the assay protocol endorsed by the FDA, DIN, and WHO is invalid. Despite decades of research and practice in this field, the quantitative quality of manufactured discs is largely unknown, potentially leading to adverse clinical outcomes. The usual standards expected for new medical
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HIV-associated disseminated Cryptococcosis- An Unusual clinical and diagnostic picture with successful cure by single dose Liposomal Amphotericin B treatment Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-25 Dr. Chhavi Gupta, Dr. Prerna Dogra, Dr. Varun Jain, Dr. Ravneet Kaur, Dr. Jyoti Bala Sharma
Cryptococcosis is an invasive, opportunistic fungal infection seen especially in human immunodeficiency virus (HIV) infected patients. Cryptococcal meningitis (CM) is the second leading cause of mortality in HIV patients. We report a case of disseminated cryptococcosis presenting with altered mental status in a newly diagnosed HIV infection. A 50-year-old with a short history of altered mental sensorium
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Diagnostic Performance of Xpert MTB/RIF Ultra in Detecting Mycobacterium tuberculosis and Rifampicin Resistance in AFB Smear-negative Pulmonary and Extrapulmonary Tuberculosis Samples in Malaysia Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-24 Azura Mohd Noor, Sumarni Mohd Ghazali, Zamzurina Abu Bakar, Izayu Nurfarha Ruzan
Rapid and highly accurate diagnostic tools are critically needed to diagnose Mycobacterium tuberculosis and rifampicin resistance in AFB smear-negative samples. In this study, we evaluated the diagnostic performance of Xpert MTB/RIF Ultra (Ultra) as a rapid test to diagnose tuberculosis in smear-negative cases in Malaysia. A retrospective study of 1960 smear-negative pulmonary and extrapulmonary samples
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A novel multiplex real-time PCR assay for the detection of cytomegalovirus, Epstein-Barr virus, herpes simplex virus 1/2 and strategies for application to blood screening Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-24 Yushan Xu, Yan Lv, Mengjiao Lin, Miaomiao Li, Dawei Cui, Yongjun Wang, Cuifen Shen, Jue Xie
A multiplex real-time PCR has been developed to simultaneously detect transfusion-transmissible pathogens cytomegalovirus, Epstein-Barr virus and herpes simplex virus, as well as to provide sample quality testing, for the conserved regions of the cytomegalovirus UL123 gene, the Epstein-Barr virus BKRF1 gene, and the herpes simplex virus 1/2 UL30 gene, tested on 500 blood donors and 320 transfusion
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Colonization of the gut mucosa of colorectal cancer patients by pathogenic mucosa-associated Escherichia coli strains Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-22 Roghayeh Nouri, Alka Hasani, Kourosh Masnadi Shirazi, Fatemeh Yeganeh Sefiadn, Fariba Naeimi Mazraeh, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee
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Pertussis outbreak in children hospitalized in Rabat (Morocco) Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-20 Redouane Mammar Bennai, Amal Zouaki, Ghizlane El Amin, Abdeljalil Rahani, Jalila Zirar, Myriam Seffar, Karim Soully, Chafik Mahraoui, Hakima Kabbaj
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SYSTEMATIC REVIEW AND META-ANALYSIS: ASSESSING THE ACCURACY OF RAPID IMMUNOCHROMATOGRAPHIC TESTS IN DENGUE DIAGNOSIS Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-20 Jéssica V.L. Macêdo, Alberto G.S. Júnior, Maria D.L. Oliveira, César A.S. Andrade
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Comparison of two automated commercial assays for routine detection of anti-hepatitis E Virus IgM antibodies in clinical samples Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-20 Adrien Becquart, Aurélie Guigon, Alexandre Regueme, Pauline Coulon, Laurence Bocket, Didier Hober, Enagnon Kazali Alidjinou
Diagnosis of hepatitis E virus (HEV) infection relies first on detection of IgM antibodies (Ab), sometimes completed with HEV RNA detection. This study aimed to compare the performance of two automated anti-HEV IgM Ab assays. Correlation between Virclia® (Vircell) and Liaison® (Diasorin) assays was carried out on 178 routine clinical samples. Both assays were run on 67 samples from HEV RT-PCR (Altona)
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Investigation of patients with new infection of echinococcal cyst in Sardinia, Italy Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-17 Cinzia Santucciu, Elena Bozzi, Stefano Profili, Alberto Porcu, Giovanna Masala, Sergio Babudieri, Scilla Mastrandrea
Cystic Echinococcosis (CE) is a zoonotic disease caused by the larval stage of a tapeworm of Taeniidae family, genus Echinococcus and species (s.l.). CE is a worldwide public health problem and is highly incident in all Mediterranean areas.
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Assessing the performance and utility of targeted next-generation sequencing for screening and genotyping of human papillomaviruses Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-16 Jung Hoon Lee, Yun Mi Park, Jimyung Kim, Gye Cheol Kwon, Seon Young Kim
A next-generation sequencing (NGS)-based Ezplex HPV NGS kit (SML Genetree, Seoul, Korea) was used for human papillomavirus (HPV) screening. Of 885 cervical swab samples, HPV was detected in 162 samples. High-risk HPVs were detected in 82 samples, and other types of HPV were detected in 13 samples (HPV86, 71, 102, 91, and 114). At the read depth ≥ 500, NGS results exhibited 100% agreement among repeated
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Shorter versus Longer Duration of Antimicrobial Therapy for Early Lyme Disease: A Systematic Review and Meta-Analysis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-15 Maria Meritxell Roca Mora, Luisa Marin Cunha, Amanda Godoi, Isabelle Donadon, Mariana Clemente, Patrícia Marcolin, Andres Valenzuela, Gary P. Wormser
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Rapid multiplex real-time PCR assay using a portable device for the detection of oral pathogens Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-15 Wit Yee Wint, Mayu Miyanohara, Hidenori Yamada, Takako Nakatsuka, Masaaki Okamoto, Koufuchi Ryo, Tomoko Tanaka, Nobuhiro Hanada, Takatoshi Murata
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Taming Pseudomonas aeruginosa AM26 the barbarian: Targeting the PQS quorum sensing network using crude mandarin extract Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-11 Yukti Oza, Rohit Patel, Dhara Patel, Arpit Shukla
Pseudomonas aeruginosa, one of the most notorious organisms, causes fatal diseases like-, meningitis, pneumonia as well as worsens the prognosis of cystic fibrosis patients. It is also multi-drug resistant and resists a wide range of antibiotics. Attempts have been made to reduce its virulence/pathogenic potential using a number of organic compounds. For this purpose, the Quorum sensing (QS) system
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Diagnosis of an immunocompetent adult with acute headache and fever as Epstein–Barr virus encephalitis by mNGS of cerebrospinal fluid Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-10 Yan Yu, Bingqian Zhuo, Wei Xu, Lanhui li, Xinyue Jin, Youqi Ji, Yumei Ge
Complicated case with fever or headache of unknown origin is currently one of the main challenges in clinical diagnosis. A retrospective analysis was conducted on a 27-year-old female patient hospitalized with headache and fever, and the pathogen species were ultimately determined by metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF). The culture results of CSF showed no bacterial
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Improved heat shock method for extracting total RNA from nasopharyngeal swab samples even with low viral load Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-10 Hannan Khodaei, Leila Azimi, Abbas Akhavan Sepahy, Fatemeh Ashrafi, Maryam Rajabnejad
This study aimed to improve the heat shock method as a cost-effective and time-efficient for total RNA extraction. We compared the effectiveness of two total RNA extraction methods by using Real-Time PCR for nasopharynx swabs. Include: I; use of a commercial total RNA extraction kit as a standard. II; utilized a modified heat shock method (MHS). Time, centrifuge speed and duration, proteinase K, and
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Comparative Evaluation of Two Molecular Multiplex Syndromic Panels with Acute Gastroenteritis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-10 Kuenyoul Park, Bo-Moon Shin
We compared the Allplex Gastrointestinal V/B1/B2 Assays and Seeplex Diarrhea V/B1/B2 ACE Detection Assays in patients with acute gastroenteritis (AGE). Of the total 432 specimens, 48.8% and 54.9% samples were positive for any bacterial or viral target using Seeplex and Allplex, respectively ( = 0.002). The overall percent agreement (OPA) between the two panels was >95% and the lowest OPA was 95.4%
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Clinical description of dengue and chikungunya virus infections amongst acute febrile patients in a malaria endemic area of Mfou, the Centre region of Cameroon Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-01 Fredy Brice Simo Nemg, Rodrigue Ngape Akoue, Maurice Demanou
This study aims to determine the frequency and clinical manifestations of dengue and chikungunya viral infections in the district hospital of Mfou, Centre region of Cameroon where malaria is endemic. Blood samples were collected from suspected cases and tested for Plasmodium parasites and for the molecular detection of viral RNAs (dengue, zika and chikungunya viruses) using TRIOPLEX qPCR. A total of
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Bacitracin agar vs. oleandomycin disk supplemented chocolate agar for the recovery of Haemophilus influenzae in diagnostic samples: a prospective comparison Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-02 Patrick Forstner, Christina Maria Laireiter, Simone Friedl, Ivo Steinmetz, Karl Dichtl
is an important pathogen able to cause various forms of respiratory and invasive disease. To provide high sensitivity for detection, culture media must inhibit growth of residential flora from the respiratory tract. This study aimed to identify and compare the diagnostic and economic advantages of using bacitracin containing selective agar (SEL) or oleandomycin disk supplemented chocolate agar (CHOC)
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Nine years of imported malaria in a teaching hospital in Belgium: Demographics, clinical characteristics, and outcomes Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-02 Ratovonjanahary T, Danwang C, Robert A, Yombi JC
Imported malaria is often misdiagnosed due to the aspecific symptoms and lack of familiarity among clinicians. This study aims to evaluate a decade-long trend of imported malaria cases in a Belgian teaching hospital by analyzing demographics, clinical characteristics, and outcomes. Medical records of 223 patients with confirmed malaria diagnoses between 2010 and 2019 were analyzed. Most patients were
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Cerebrospinal fluid drain infection caused by pandrug-resistant Staphylococcus epidermidis successfully treated with ceftaroline in combination with fosfomycin and vancomycin Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-02 Eugenia MAGRINI, Emanuele RANDO, Paola DEL GIACOMO, Elena MATTEINI, Gabriele Maria LEANZA, Flavia SANMARTIN, Andrea CARBONE, Giuseppe MAIURO, Alex DUSINA, Antonella CINGOLANI
External ventricular drain-related cerebrospinal fluid infection represents a fearsome complication of neurosurgical interventions. Although vancomycin represents the standard of care for methicillin-resistant CoNS healthcare-associated ventriculitis, resistance phenomena have been described. We reported a case of a persistent external ventricular fluid drain infection after device removal by pandrug-resistant
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Gardnerella vaginalis ventilatory acquired pneumonia among patients with trauma Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-01 Valentin De Villiers De La Noue, Lucien Brasme, Odile Bajolet, Vincent Legros
() is a bacterium rarely responsible for systemic infections and is exceptionally isolated from bronchopulmonary samples. Here, we report here two patients with trauma who were diagnosed with a ventilatory acquired pneumonia (VAP) via mini bronchoalveolar lavage (mini-BAL). According to our observations, was the only microorganism with a significant threshold and the identification was obtained by
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Multi-locus sequence typing of geographically and temporally diverse strains of Mycoplasma hominis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-02-01 Safa Boujemaa, Gurparsad Singh Suri, Gurleen Kaur
This study aimed to investigate the genetic diversity of 108 geographically and temporally diverse strains of using a multi-locus sequence typing scheme (MLST). We extracted MLST data of 87 strains from PubMLST database and retrieved MLST gene sequences from 21 complete genomes of available in GenBank database. MLST scheme identified 65 Sequence types (STs), which were grouped into five clonal complexes
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Factors associated with the persistence of human papillomavirus after surgery in patients with cervical cancer Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-30 Qing Wang, Fang-yue Zhou, Jing-xin Ding
Objective To determine the rate of human papillomavirus (HPV) persistence after surgery in patients with cervical cancer, and to analyze the factors associated with HPV persistence and viral load after surgery. Methods Medical records of women who underwent surgery for treatment of cervical cancer between 1 January 2018 and 30 June 2019 at Obstetrics and Gynecology Hospital of Fudan University in Shanghai
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Soft tissue infection and osteomyelitis caused by Mycobacterium farcinogenes after heart surgery: case report and literature review of human cases Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-26 Fei Ye, Menglin Luo, Peng Gu, Xuelian Hu, Junyan Yan, Renguo Wen, Jingbin Huang
Mycobacterium farcinogenes (M. farcinogenes) is rapidly growing mycobacterium, belonging to non-tuberculous mycobacterial (NTM).M. farcinogenes is an exceedingly rare causative agent of human infection. Only seven cases with M. farcinogenes infections in humans were reported. This is a case of soft tissue infection and osteomyelitis caused by M. farcinogenes after heart surgery. Microbial identification
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Altered haematological parameters in children with malaria infection, a systematic review and meta-analysis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-28 Umra Fatima Zuberi, Sana Aqeel, Faizeaab Hashmi, Wajihullah Khan
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Comparative Analysis of Different Loop Types for Urine Culture Collection: Implications for Quantitative Bacterial Growth and Culture Results Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-24 Ayse Nur CEYLAN, Ali TOPRAK, Mehmet Ziya DOYMAZ, Bilge SÜMBÜL
We hypothesized that the loop material and size could affect the results of the culture when compared to the calibrated pipette. A total of 484 urine samples were included in the study, and each sample was plated by using different loop types and the calibrated pipette. The bacterial counts per milliliter were calculated and compared, with a focus on the important cutoff values of 10³ and 10⁴ CFU/ml
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The fully automated Sysmex XN-31 hematology analyzer can detect bloodstream form Trypanosoma brucei Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-25 Tania Khartabil, Ron HN van Schaik, Jurgen R. Haanstra, Rob Koelewijn, Henk Russcher, Jaap J. van Hellemond
Background For fully automated detection and quantification of Plasmodium parasites, Sysmex developed the XN-31 hemocytometer. This study investigated whether the XN-31 can also detect and quantify bloodstream form trypanosomes (trypomastigotes). Methods Axenic cultures of Trypanosoma brucei brucei were used to prepare two dilution series of trypomastigotes in the whole blood of a healthy donor, which
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Identification of prosthetic joint infections with 16S amplicon metagenomic sequencing – comparison with standard cultivation approach Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-17 Aleksander Mahnic, Mitja Rak, Rihard Trebše, Maja Rupnik, Andrej Cőr
Prosthetic joint infections (PJIs) are commonly diagnosed via culture-based methods, which may miss hard-to-grow pathogens. This study contrasts amplicon metagenomic sequencing (16S AS) with traditional culture techniques for enhanced clinical decision-making. We analyzed sonicate fluid from 27 patients undergoing revision arthroplasty using both methods, emphasizing the distinction between contaminants
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Colistin heteroresistance in Citrobacter freundii clinical isolates from Republic of Korea Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-17 Jong Hyun Shin, Dongwoo Shin, Ki Tae Kwon, Kwan Soo Ko
We investigated colistin heteroresistance in Citrobacter freundii isolates from Korean hospitals. Using population analysis profiling (PAP), we detected colistin heteroresistance in 31.3% of isolates. Among these, ST217 was the most prevalent clone (58.5%), particularly within colistin-heteroresistant isolates (80.0%). Interestingly, the second most common clone, ST248, was not found in heteroresistant
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Eubacterium bacteremia – a retrospective observational study of a seldom found anaerobic pathogen Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-13 Anna Bläckberg, Karin Holm, Karin Liderot, Bo Nilson, Torgny Sunnerhagen
Background Human infections due to Eubacterium are rare and knowledge of the condition is limited. This study aimed to describe clinical characteristics and outcome in patients with Eubacterium bacteremia. Methods Episodes of Eubacterium bacteremia were identified through the clinical microbiology laboratory in Lund, Sweden. Medical records were retrospectively reviewed. Blood isolates of Eubacterium
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(1-3)-ß-D-glucan for the diagnosis of Nocardia infection in solid organ transplant recipients Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-14 Margot Paumier, Julien Coussement, Marie Matignon, Cécile Chauvet, Nicolas Bouvier, Arthur Poncelet, Jacques Dantal, Anne Scemla, Helga Ceunen, Eric Van Wijngaerden, Nassim Kamar, Martha T. van der Beek, Herman F. Wunderink, Julien De Greef, Sophie Candon, Marie-Elisabeth Bougnoux, David Lebeaux
Abstract not available
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Cellulitis and bacteremia caused by the fish pathogen, Streptococcus iniae, in an immunocompromised patient: Case report and mini-review of zoonotic disease, lab identification, and antimicrobial susceptibility Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-18 Andrea Kirmaier, Leslie Blackshear, Matthew Shou Lun Lee, James E. Kirby
Streptococcus iniae is a fish pathogen that can also infect mammals including dolphins and humans. Its prevalence in farmed fish, particularly tilapia, provides potential for zoonotic infections, as documented by multiple case reports. Systematic clinical data beyond cellulitis for S. iniae infection in humans, including antimicrobial susceptibility data, are unfortunately rare. Here, we present a
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The value of calcofluor white in the diagnosis of invasive fungal diseases Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-14 Shuya Zhang, Zhongbo Chen, Zaichun Deng, Panfeng Fu, Yiming Yu, Qidong Zhuang, Xuechan Yu, Hui Chen, Anqi Li, Lin Zheng
Purpose To evaluate the value of calcofluor white in the diagnosis of invasive fungal disease (IFD). Methods A total of 84 patients with possible pulmonary fungal infection who underwent bronchoscopy with bronchoalveolar lavage fluid (BALF) were included. All BALF specimens were subjected to Calcofluor white (CFW), potassium hydroxide (KOH) and Gram stains. Results CFW has the most sensitivity than
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Emerging of multidrug-resistant Pseudomonas guariconensis with blaVIM-2 in an asymptomatic bacteriuria patient: a rare clinical presentation Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-07 Choseok Yoon, Yangsoon Lee
This case presents the clinical and genomic aspects of a rare and multidrug-resistant Pseudomonas guariconensis isolate carrying blaVIM-2 and highlights the need for heightened awareness in healthcare facilities. A 63-year-old woman underwent surgery for the diagnosis of a paraspinal abscess and infectious spondylitis. During hospitalization, the patient was diagnosed with heart failure exacerbation
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Impact of restrictive urinalysis reflex to culture criteria at a large community hospital Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-10 Christian F Caveness, Alison I Orvin, Christopher W Ingram, Jeannette L Bouchard
Treatment of asymptomatic bacteriuria (ASB) is a common, but often unnecessary, practice. Our objective was to determine the impact of restrictive urinalysis reflex to culture (UARC) criteria on rate of urine cultures (UC) ordered and ASB treatment. Criteria were modified from positive leukocyte esterase, positive nitrites, or white blood cells (WBC) >10 cells to only WBC >10 cells. This pre-post study
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Comparison of the Alinity M STI with the GeneXpert CT/NG for the detection of sexually transmitted microorganisms Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-04 Eric Farfour, Cyril Kitous, David Zucman, Pauline Touche, Kewin Panel, Erwan Fourn, Marc Vasse
We assess the performances of the Alinity M STI assay (Abbott Molecular) in comparison to the Xpert CT/NG assay (Cepheid). We first retrospectively used a collection of 70 frozen samples of which 33, 31, and 6 were positives for Chlamydia trachomatis (CT), Neisseria gonorrhoea (NG), and both micro-organisms respectively. The Alinity M STI and the Xpert CT/NG results were in accordance for all. The
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Prevalence and molecular characteristics of heterogeneous vancomycin intermediate Staphylococcus aureus in a tertiary care center of northern China Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-04 Xin Cheng, Liyan Ma, Yaru Wang, Wei Sun, Jianrong Su
The use of glycopeptide medications may decline in line with the annual decline in methicillin-resistant Staphylococcus aureus (MRSA) detection rates in China. The rate of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA)detection may be impacted by this. However, there is currently a dearth of information on the incidence of hVISA in China. This study aims to analyze the recent epidemiology
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Intermethod comparability analyses of gepotidacin antimicrobial susceptibility tests using a large collection of globally collected Escherichia coli and Staphylococcus saprophyticus clinical isolates Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-06 S.J. Ryan Arends, Deborah Butler, Nicole Scangarella-Oman, Mariana Castanheira, Rodrigo Mendes
Gepotidacin (GSK2140944) is a novel, bactericidal, first in class triazaacenaphthylene bacterial type II topoisomerase inhibitor in development for the treatment of uncomplicated urinary tract infections and gonorrhea. The performance of several antimicrobial susceptibility methods (broth microdilution, gradient diffusion, and disk diffusion) for gepotidacin were evaluated using over 5,800 recent Escherichia
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Diagnostic Value of MicroRNAs in Active Tuberculosis based on Meta-analysis and Enrichment Analyses Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-03 Shuoyi Yao, Bin Liu, Xinyue Hu, Yun Tan, Kun Liu, Meng He, Bohan Wu, Namra Ahmad, Xiaoli Su, Minhan Yi, Yuan Zhang
Background Tuberculosis (TB) infection remains a crucial global health challenge, with active tuberculosis (ATB) representing main infection source. MicroRNA (miRNA) has emerged as a potential diagnostic tool in this context.. This study aims to identify candidate miRNAs for ATB diagnosis and explore their possible mechanisms. Methods Differentially expressed miRNAs in ATB were summarized in qualitative
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Rapid diagnosis of Aspergillus fumigatus endocarditis using mNGS assay: A case report and review of the literature Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-01 Xiaowei Li, Dandan Li, Dongsheng Han
Fungal endocarditis is caused mainly by Candida albicans and Aspergillus spp. and was first reported in the 1950s. Natural-valve endocarditis caused by Aspergillus is relatively uncommon. In this case, a 56-year-old male patient was admitted to the hospital on account of a cough accompanied by chills and fever and ineffective self-medication. Infective endocarditis was initially suspected based on
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Cerebrospinal fluid metagenomics has greatest added value as a test for Powassan virus among patients in New England with suspected central nervous system infection Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2024-01-04 Erik H. Klontz, Isaac H. Solomon, Sarah E. Turbett, Jacob E. Lemieux, John A. Branda
Cerebrospinal fluid (CSF) metagenomic next generation sequencing (mNGS) can detect diverse pathogens in patients with central nervous system infection. Due to its high cost and unclear clinical utility, it is typically reserved for patients with unrevealing routine workups. A multi-center retrospective analysis of real-world CSF mNGS was performed involving orders between 2017 and 2022 at a large New
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Clinical Performance of BALF Droplet Digital PCR for Differential Diagnosis of Pneumocystis jirovecii Pneumonia and Pneumocystis jirovecii Colonization Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-31 Wenjie Bian, Ying Shang, Lili Zhao, Keqiang Wang, Ran Li, Yanwen Chen, Xinqian Ma, Yukun He, Wenyi Yu, Xi Chen, Chunyu Liu, Wentao Ni, Zhancheng Gao
Background Accurate differentiation between Pneumocystis jirovecii (Pj) infection and colonization is crucial for effective treatment. Methods From September 2016 to June 2022, 89 immunocompromised patients with unexplained lung infiltrates and clinical suspicion of Pj pneumonia were enrolled at Peking University People's Hospital. Bronchoalveolar lavage fluid (BALF) of these patients were detected
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Postoperative mediastinitis and sternal osteitis after cardiac surgery caused by Mycoplasma hominis: A case report Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-31 Gen Li, Wanjun Yu, Yangqin Ye, Peng Han, Liqiong Chen, Wenyan Zhang, Chi Xu, Lieying Fan
Background Mediastinitis and sternal osteitis are critical complications in cardiac surgery. Cases of these complications caused by Mycoplasma hominis are extremely rare. Case presentation We present a case of mediastinitis and sternal osteitis caused by M. hominis infection following ascending aortic replacement surgery. Whole gene sequencing analysis suggested the genitourinary tract as the most
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The utility of smartphone-based quantitative analysis of SARS-CoV-2-specific antibody lateral flow assays Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-24 Yoshifumi Uwamino, Shiho Tanaka, Ayako Shibata, Toshinobu Kurafuji, Hideki Ishihara, Yasunori Sato, Hiromichi Matsushita
Objectives Although antibody measurements using lateral flow assay (LFA) kits are convenient, they usually require a specialized reader for quantification. However, a smartphone-based quantification application can be used as a reader for LFA kits. We investigated the quantification ability of the application for SARS-CoV-2-specific antibodies. Methods Eight hundred frozen serum samples from 100 healthcare
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Metagenomic next-generation sequencing on bronchoalveolar lavage fluid to contribute to diagnosis of subclinical pulmonary tuberculosis with scarce sputum and negative smear in a patient mimicking adult- onset still's disease: A case report Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-27 Yanchun Guan, Xiao Ma, Xiangnan Sun, Hongfeng Zhang
Extremely high serum ferritin, which is regarded as a marker of adult-onset still's disease (AOSD), has been rarely observed in patients with TB. We report a case of TB diagnose by metagenomic next-generation sequencing(mNGS) who presented with clinical criteria of AOSD and extreme hyperferritinemia, which posed a diagnostic confusion. TB presenting with major clinical criteria of AOSD should be notable
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Evaluating the diagnostic performance of Liaison® chemiluminescence assay as screening tool for detection of acute Epstein-Barr infection: A comparative study Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-24 Jordi Tortosa-Carreres, Carmen Lloret-Sos, Jose Miguel Sahuquillo-Arce, Pedro Suárez-Urquiza, Josep Prat-Fornells, Jose Miguel Molina-Moreno, Amparo Alba-Redondo, Maria Luisa Martínez-Triguero, Cristina Aguado-Codina, Begoña Laiz-Marro, Jose Luis López-Hontangas
The present investigation assessed the Liaison® diagnostic performance in detecting Epstein-Barr (EBV) IgM-VCA in a large patient cohort, considering age and symptomatology. VIDAS® were employed as a benchmark for acute EBV infection. The study also probed other coexisting conditions and potential cross-reactivity for error sources. A total of 1311 samples were analyzed, with notable associations found
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Background seropositivity to Jamestown Canyon virus can lead to diagnostic confusion Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-22 Rachel Gnanaprakasam, Gary P. Wormser, Marina Keller
Background seropositivity rates for specific antibodies to Jamestown Canyon Virus (JCV) can exceed 25 % in certain geographic areas in the United States. This can potentially lead to diagnostic confusion, as apparently illustrated by a patient from New Jersey with Powassan virus encephalitis, who also tested positive for antibodies to JCV.
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Analytical performance of the Abbott ID NOW 2.0 assay for SARS-CoV-2 detection in clinical samples from symptomatic patients Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-21 Eunhee Han, Seungok Lee, Hoon Seok Kim, Haein Yu, Gun Dong Lee, Yonggoo Kim, Myungshin Kim
We evaluated the analytical performance of ID NOW™ COVID-19 2.0 assay versus conventional real-time reverse transcription-polymerase chain reaction (RT-PCR) using a total of 792 clinical samples from nasopharyngeal and oropharyngeal swabs, stored in frozen universal transport medium samples. Positive percent agreement (PPA) and negative percent agreement of ID NOW were 97.6 % and 100 %, respectively
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Systemic ciprofloxacin treatment of multidrug-resistant Aggregatibacter actinomycetemcomitans in severe periodontitis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-15 Thomas E. Rams, Ira S. Freedman, Susan M. Chialastri, Jørgen Slots
An adult periodontitis patient treated with mechanical/surgical therapy experienced gingival necrosis and granulomas post-treatment. Aggregatibacter actinomycetemcomitans, a tissue-invasive pathogen, was recovered and multidrug-resistant but susceptible to ciprofloxacin. Systemic ciprofloxacin eliminated A. actinomycetemcomitans with marked clinical improvement. Ciprofloxacin may be prescribed for
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Early-stage neutralizing antibody level associated with the re-positive risk of Omicron SARS-CoV-2 RNA in patients recovered from COVID-19 Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-17 Tingting You, Weiqiang Huang, Xiaoxiao Huang, Er Li, Yongping Liu, Lizhi Xue, Xiaoqiang Chen, Shourong Liu, Fang Liu, Jianfeng Bao
Post-discharge re-positivity of Omicron SARS-CoV-2 is challenging for the sufficient control of this pandemic. However, there are few studies about the risk of re-positivity. We aimed to explore the association of neutralizing antibodies (nAbs, AU/mL) with the incidence of re-positivity among patients recovered from COVID-19. A retrospective cohort study selected 318 Omicron-infected patients was conducted
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High frequency of isoniazid and fluroquinolone resistance among patients with rifampicin sensitive tuberculosis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-12 Parul Jain, Rashmi Ratnam, Senjuti Sengupta, Urmila Singh, Vijay Kumar, Amita Jain
This study was done to determine frequency of isoniazid (INH) and fluoroquinolones FQ resistance among rifampicin sensitive strains of Mycobacterium tuberculosis and to study their mutation patterns. Retrospective analysis was done for samples with M. tuberculosis detected by Cartridge based NAAT (CBNAAT). They were tested sequentially by first line (FL) and second line - line probe assay (SL-LPA)
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Non-specific signals in real-time RT-PCR for detecting respiratory viruses Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-17 Gannon C.K. Mak, Derek L.L. Hung
We describe our experiences in investigating the origin of non-specific signals during the development phase of a multiplex PCR assay for respiratory viruses. After ruling out various sources of error, eventually we discovered the non-specific signal was related to the particular lot of the PCR kit.
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Comparable detection of HPV using real-time PCR in paired cervical samples and concentrated first-stream urine collected with Colli-Pee device Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-12 Pornjarim Nilyanimit, Surasith Chaithongwongwatthana, Shina Oranratanaphan, Nimesh Poudyal, Jean-Louis Excler, Julia Lynch, Sompong Vongpunsawad, Yong Poovorawan
We compared high-risk human papillomavirus (HPV) detection on first-stream urine from self-sampled collection device (Colli-Pee) and same-day clinician-collected cervical swab in 240 women. Testing with automated cobas 4800 system showed 96.7 % concordance (198 concordant-negative, 34 concordant-positive, Cohen's kappa=0.87). HPV testing on Colli-Pee urine offers advantages for acceptable non-invasive
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SARS-CoV-2 emergency use authorization published sensitivity differences do not correlate with positivity rate in a hospital/reference laboratory setting Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-10 Joseph H. Blommel, Luigi Boccuto, Diana S. Ivankovic, Sara M. Sarasua, Benjamin R. Kipp, Brad S. Karon
During the first year of the COVID-19 pandemic skyrocketing demand for testing in the United States, coupled with supply chain issues, necessitated the use of multiple SARS-CoV-2 molecular testing platforms at many health centers. At our institution these platforms consisted of 8 ordered services for sample triage, using 9 emergency use authorized (EUA) SARS-CoV-2 RNA nucleic acid amplification tests
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Assessment of in vitro colistin susceptibility of Carbapenem-resistant clinical Gram-negative bacterial isolates using four commercially available systems & Whole-genome sequencing: A diagnostic accuracy study Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-07 Mohit Bhatia, Varun Shamanna, Geetha Nagaraj, Pratima Gupta, Balram Ji Omar, , Ranjana Chaudhary, K.L. Ravikumar
Aim To analyze the diagnostic utility of commercially available platforms and Whole-genome sequencing (WGS) for accurate determination of colistin susceptibility test results. Material & Methods An exploratory diagnostic accuracy study was conducted in which sixty carbapenem-resistant Gram-negative bacteria were subjected to identification and AST using MALDI-TOF MS & MicroScan walkaway 96 Plus. Additional
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Deciphering Intermediate Nugent scores: Utility of Real-time PCR for Bacterial Vaginosis diagnosis Diagn. Microbiol. Infect. Dis. (IF 2.9) Pub Date : 2023-12-02 R Mala, S Malhotra, N Singh, S Gupta, D A Upadhyay, A Kapil, S Sood
Purpose To optimize real-time PCR assays for diagnosis of Bacterial Vaginosis (BV) and determine cut-off loads by ROC analysis for Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp. as compared to Nugent scoring (Gold standard) in clinical samples. Results Out of 125 women, 34 were positive, 26 intermediate and 65 negative for BV by Nugent scoring. All three real-time PCR assays were found