样式: 排序: IF: - GO 导出 标记为已读
-
Trilobatin contributes to the improvement of myopathy in a mouse model of Duchenne muscular dystrophy Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2024-03-13 Túlio de Almeida Hermes, Paula Fratini, Beatriz Godinho Nascimento, Laís Leite Ferreira, Giuliana Petri, Fernando Luiz Affonso Fonseca, Alzira Alves de Siqueira Carvalho, David Feder
Duchenne muscular dystrophy (DMD) occurs due to genetic mutations that lead to a deficiency in dystrophin production and consequent progressive degeneration of skeletal muscle fibres, through oxidative stress and an exacerbated inflammatory process. The flavonoid trilobatin (TLB) demonstrates antioxidant and anti‐inflammatory potential. Its high safety profile and effective action make it a potent
-
TGFβR1 inhibition drives hepatocellular carcinoma proliferation through induction of toll-like-receptor signalling Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2024-02-08 Fatma El Zahraa Ammar Mohamed, Bedair Dewidar, Tao Lin, Matthias P. Ebert, Steven Dooley, Nadja M. Meindl-Beinker, Seddik Hammad
Transforming growth factor (TGF)-β and toll-like receptors (TLRs) have been shown to independently modulate the proliferation of hepatocellular carcinoma (HCC). Since a direct cross-talk between these two signalling pathways in HCC has not been clearly described before, we aimed here to explore the possibility of such interaction. A human HCC tissue array (n = 20 vs. four control samples), human HCC
-
MiR-22-3p facilitates bone marrow mesenchymal stem cell osteogenesis and fracture healing through the SOSTDC1-PI3K/AKT pathway Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-12-28 Chunqiu Wang, Xinguo Wang, Hui Cheng, Jiahu Fang
Bone fractures are the most common form of musculoskeletal trauma worldwide. Numerous microRNAs (miRNAs) have been suggested to be participants in regulating bone-related diseases. Recent studies revealed the regulatory role of miR-22-3p in osteogenic differentiation, but its role in fracture healing has not been investigated previously. Here, a rat femoral fracture model was established, Bone marrow
-
Increased accumulation of the advanced glycation endproduct Ne(carboxymethyl) lysine in the intramyocardial vasculature in patients with epicarditis Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-12-08 U Baylan, A Baidoshvili, S Simsek, CG Schalkwijk, HWM Niessen, PAJ Krijnen
Advanced glycation end-products (AGEs) are implicated in the pathogenesis of vascular disease. In previous studies we have found increased deposition of N(e)-(carboxymethyl)lysine (CML) in intramyocardial vasculature in the heart in acute myocardial infarction and myocarditis. It is known that the process of inflammation plays a role in the formation of AGEs. In this study we have explored the presence
-
Circular RNA circVAPA modulates macrophage pyroptosis in sepsis-induced acute lung injury through targeting miR-212-3p/Sirt1/Nrf2/NLRP3 axis Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-12-06 Yanjing Huang, Jinquan Lin, Zhiwei Wu, Yiming Li
Sepsis-induced acute lung injury (ALI) is an inflammatory condition involving the pyroptosis of macrophages. This study investigated the role of circular RNA hsa_circ_0006990 (circVAPA) in regulating macrophage pyroptosis in ALI and the underlying mechanisms. The expression pattern of circVAPA was examined in the mouse model of ALI and in the LPS-treated RAW264.7 macrophage cell line. Lung tissue damage
-
Anti-inflammatory and healing effect of the polysaccharidic extract of Opuntia ficus-indica cladodes in cutaneous excisional wounds in rats Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-11-22 Beatriz Lima Adjafre, Iásly Costa Lima, Ana Paula Negreiros Nunes Alves, Rafael Aires Lessa, Arcelina Pacheco Cunha, Maria Gonçalves Pereira, Ana Maria Sampaio Assreuy, Mário Rogério Lima Mota
This study aimed to investigate the anti-inflammatory and wound healing effects of the polysaccharide extract from Opuntia ficus-indica cladodes (TPL-Ofi) using a rat cutaneous wound model. After anaesthesia, four 7-mm-diameter dorsal wounds per animal (n = 6/group for each experimental day of evaluation) were created in female Wistar rats using a surgical punch. The animals were treated topically
-
Exploring effect of M2 macrophages on experimental full-thickness wound healing in streptozotocin-induced diabetic rats Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-11-15 Parmis Khoshnoudi, Soroush Sabiza, Mohammad Khosravi, Babak Mohamadian
Diabetes mellitus is one of the most prevalent medical conditions, in both humans and animals. People with diabetes mellitus often experience slower than normal wound healing, making it a serious health concern. This study investigates the effect of M2 differentiated macrophages on full-thickness wound healing in white Westar rats exposed to streptozocin 70 mg/kg. A full-thickness skin defect with
-
Notoginsenoside R1 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells via ERα/GSK-3β/β-catenin signalling pathway Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-10-30 Wei Lu, Yuanxin Shi, Minglei Qian
Human bone marrow mesenchymal stem cells (hBMSCs) are attractive therapeutic agents for bone tissue regeneration owing to their osteogenic differentiation potential. Notoginsenoside R1 (NGR1) is a novel phytoestrogen with diverse pharmacological activities. Here, we probed whether NGR1 has an effect on the osteogenic differentiation of hBMSCs. EdU, CCK-8 and Transwell assays were used to measure proliferation
-
Interaction between mitochondrial homeostasis and barrier function in lipopolysaccharide-induced endothelial cell injury Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-10-12 Weiwei Zhu, Xiaojing Liu, Liqing Luo, Xiao Huang, Xiaozhi Wang
This study aimed to investigate the effects of mitochondrial homeostasis on lipopolysaccharide (LPS)-induced endothelial cell barrier function and the mechanisms that underlie these effects. Cells were treated with LPS or oligomycin (mitochondrial adenosine triphosphate synthase inhibitor) and the mitochondrial morphology, mitochondrial reactive oxygen species (mtROS), and mitochondrial membrane potential
-
British Society for Matrix Biology Spring Meeting 2023: Vascular Inflammation and the Extracellular Matrix. Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-10-01
-
Histomorphometric lung density evaluation of Immulina treatment using a murine influenza pneumonia model Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-09-26 Floyd D. Wilson, Tahir M. Mir, Mohammad K. Ashfaq, Jin Zhang, Nirmal D. Pugh, Ikhlas A. Khan, Lanny W. Pace, Frederic J. Hoerr
Histomorphometric lung density measurements were used to evaluate the effects of Immulina on mouse pneumonia. Mice were intra-nasally exposed to H1N1 influenza virus at a dose of 5 × 104 PFU/50 μL/mouse. Lung density was measured using the NIH ImageJ software program. Density values were compared to semiquantitative pneumonia severity scores. Lung photomicrographs were evaluated at 25-×, 40-× and 400-×
-
Inhibition of inflammation and infiltration of M2 macrophages in NSCLC through the ATF3/CSF1 axis: Role of miR-27a-3p Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-08-28 Bin Zhou, Yan Xu, Li Xu, Yi Kong, Kang Li, Bolin Chen, Jia Li
Non-small cell lung cancer (NSCLC) imposes a significant economic burden on patients and society due to its low overall cure and survival rates. Tumour-associated macrophages (TAM) affect tumour development and may be a novel therapeutic target for cancer. We collected NSCLC and tumour-adjacent tissue samples. Compared with the tumour-adjacent tissues, the Activation Transcription Factor 3 (ATF3) and
-
Optimization of decellularization methods using human small intestinal submucosa for scaffold generation in regenerative medicine Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-08-25 Shumei Mineta, Shunji Endo, Tomio Ueno
Porcine small intestinal submucosa, despite its successful use as a scaffold in regenerative medicine, has innate biomechanical heterogeneity. In this study, we hypothesized that human small intestinal submucosa could be a viable alternative bio-scaffold. For the first time, we characterize submucosal extraction from human small intestine and examine appropriate decellularization methods. In total
-
Panx1 knockout promotes preneoplastic aberrant crypt foci development in a chemically induced model of mouse colon carcinogenesis Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-08-18 Sara Gomes Espírito Santo, Tereza Cristina Da Silva, Bruno Cogliati, Luís Fernando Barbisan, Guilherme Ribeiro Romualdo
Colorectal cancer, which is the third leading cause of cancer-related deaths worldwide, is a multistep disease, featuring preneoplastic aberrant crypt foci (ACF) as the early morphological manifestation. The roles of hemichannel-forming transmembrane Pannexin 1 (Panx1) protein have not been investigated in the context of colon carcinogenesis yet, although it has contrasting roles in other cancer types
-
Nek6 knockdown polarized macrophages into a pro-inflammatory phenotype via inhibiting STAT3 expression Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-07-10 Xiaoyan Wu, Ke-Qiong Deng, Huan-Huan Cai, Ziyue Zeng, Jian-Lei Cao, Lin Zhang, Zhibing Lu, Wen-Lin Cheng
Recently macrophage polarization has emerged as playing an essential role in the oathogenesis of atherosclerosis, which is the most important underlying process in many types of cardiovascular diseases. Although Nek6 has been reported to be involved in various cellular processes, the effect of Nek6 on macrophage polarization remains unknown. Macrophages exposed to lipopolysaccharide (LPS) or IL-4 were
-
mRNA expression profile reveals differentially expressed genes in splenocytes of experimental autoimmune encephalomyelitis model Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-07-10 Arshad Mehmood, Shuang Song, Xiaochen Du, Hongjing Yan, Xuan Wang, Li Guo, Bin Li
Experimental autoimmune encephalomyelitis (EAE) is a mouse model that can be used to investigate aetiology, pathogenesis, and treatment approaches for multiple sclerosis (MS). A novel integrated bioinformatics approach was used to understand the involvement of differentially expressed genes (DEGs) in the spleen of EAE mice through data mining of existing microarray and RNA-seq datasets. We screened
-
Mechanism of the HIF-1α/VEGF/VEGFR-2 pathway in the proliferation and apoptosis of human haemangioma endothelial cells Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-06-28 Wenpei Zhang, Lei Sun, Hongxia Gao, Shengquan Wang
Haemangiomas (HAs) are prevalent vascular endothelial cell tumours. With respect to the possible involvement of HIF-1α in HAs, we have explored its role in haemangioma endothelial cell (HemEC) proliferation and apoptosis. shRNA HIF-1α and pcDNA3.1 HIF-α were manipulated into HemECs. HIF-α, VEGF, and VEGFR-2 mRNA and protein levels were assessed by qRT-PCR and Western blotting. Cell proliferation and
-
A prognostic model based on regulatory T-cell-related genes in gastric cancer: Systematic construction and validation Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-06-23 Qin Tong, Yingjie Ling
Human gastrointestinal tumours have been shown to contain massive numbers of tumour infiltrating regulatory T cells (Tregs), the presence of which are closely related to tumour immunity. This study was designed to develop new Treg-related prognostic biomarkers to monitor the prognosis of patients with gastric cancer (GC). Treg-related prognostic genes were screened from Treg-related differentially
-
Clinical applications of gene therapy for rare diseases: A review Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-05-13 Ioannis Papaioannou, James S. Owen, Rafael J. Yáñez-Muñoz
Rare diseases collectively exact a high toll on society due to their sheer number and overall prevalence. Their heterogeneity, diversity, and nature pose daunting clinical challenges for both management and treatment. In this review, we discuss recent advances in clinical applications of gene therapy for rare diseases, focusing on a variety of viral and non-viral strategies. The use of adeno-associated
-
RUNX1 promotes liver fibrosis progression through regulating TGF-β signalling Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-04-17 Zhaoyang Guo, Xinxin Liu, Shulei Zhao, Fengkai Sun, Wanhua Ren, Mingze Ma
Liver fibrosis is caused by chronic liver injury. There are limited treatments for it, and the pathogenesis is unclear. Therefore, there is an urgent need to explore the pathogenesis of liver fibrosis, and to try to identify new potential therapeutic targets. For this study we used the carbon tetrachloride abdominal injection induced liver fibrosis animal model in mice. Primary hepatic stellate cell
-
Abnormal expression and role of MicroRNA-214-3p/SLC8A1 in neonatal Hypoxic-Ischaemic encephalopathy Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-04-09 Liu Yang, Li Zhang, Jing Zhu, Yuqian Wang, Ning Zou, Zhengjuan Liu, Yingjie Wang
Neonatal hypoxic-ischaemic encephalopathy (HIE) refers to brain damage caused by intra-uterine distress and asphyxia/hypoxia during the perinatal and neonatal periods. MicroRNA (MiR)-214-3p plays a critical role in cell growth and apoptosis. The aim of this study was to investigate the expression and role of miR-214-3p in neonatal HIE development, and to explore the underlying mechanisms. The expression
-
High density of FOXP3 predicts better prognosis in germinal and non-germinal centre diffuse large B-cell lymphoma Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-03-28 Asmaa M. Ahmed, Sally S. Abdel-Hakeem, Maged A. F. Amine, Etemad H. Yassin, Fatma A. M. Badary
This study highlights the prognostic impact of FOXP3 and CD68 expression in DLBCL, NOS and in its GCB and non-GCB subtypes. This may help the development of individualized therapy, prognostic prediction and therapy stratification.
-
Arginine vasopressin deficiency and conivaptan (a V1a–V2 receptor antagonist) treatment reverses liver damage and fibrosis in rats with chronic portocaval anastomosis Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-03-25 Yesenia Danyeli Navarro-Gonzalez, Javier Ventura-Juarez, Martín Humberto Muñoz-Ortega, Daniel González-Blas, Argelia Calvillo-Robedo, Manuel-Enrique Avila-Blanco, Fernando Valdez-Urias, Andrés Quintanar-Stephano
Arginine vasopressin (AVP) is a naturally occurring hormone synthesized in the hypothalamus. AVP demonstrates pro-fibrotic effects as it stimulates hepatic stellate cells to secrete transforming growth factor-β (TGF-β) and collagen. Previous work in liver cirrhotic (CCL4-induced) hamsters demonstrated that AVP deficiency induced by neurointermediate pituitary lobectomy (NIL) can restore liver function
-
Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-03-16 Chao Zeng, Jin-ke Wu, Xiaofang Lu
The aim of this study was to investigate the correlation between Silva pattern system and clinicopathological features of endocervical adenocarcinoma. Moreover, it was to find molecular markers helpful for Silva classification, and thus we also explored the expression levels of invasion, adhesion and proliferation biomarkers in cases of Silva non-invasive and invasive types. The survival based on Silva
-
British Society for Matrix Biology Autumn Meeting 2022: "Matrix in Development". Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-04-01
-
Effect of chronic exposure to fine particulate matter on cardiac tissue of NZBWF1 mice Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-03-14 Dunia Waked, Ana Clara B. Rodrigues, Thamires Moraes Silva, Victor Yuji Yariwake, Sylvia Costa Lima Farhat, Mariana Matera Veras
Epidemiological and toxicological studies have shown that inhalation of particulate matter (PM) is associated with development of cardiovascular diseases. Long-term exposure to PM may increase the risk of cardiovascular events and reduce life expectancy. Systemic lupus erythematosus (SLE) is a chronic inflammatory disease, autoimmune in nature, that is characterized by the production of autoantibodies
-
Wnt signalling in the articular cartilage: A matter of balance Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-02-26 Amandeep Kaur Gill, Peter J. McCormick, David Sochart, Giovanna Nalesso
Degradation of the articular cartilage is a hallmark of osteoarthritis, a progressive and chronic musculoskeletal condition, affecting millions of people worldwide. The activation of several signalling cascades is altered during disease development: among them, the Wnt signalling plays a pivotal role in the maintenance of tissue homeostasis. Increasing evidence is showing that its activation needs
-
miR-144-3p represses hepatocellular carcinoma progression by affecting cell aerobic glycolysis via FOXK1 Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-02-19 Binyu Xing, Cunyi Shen, Qinling Yang, Zheng Wang, Wenjun Tan
Aerobic glycolysis is a unique mark of cancer cells, which enables therapeutic intervention in cancer. Forkhead box K1 (FOXK1) is a transcription factor that facilitates the progression of multiple cancers including hepatocellular carcinoma (HCC). Nevertheless, it is unclear whether or not FOXK1 can affect HCC cell glycolysis. This study attempted to study the effect of FOXK1 on HCC cell glycolysis
-
Arnica montana L. associated with microcurrent accelerates the dermis reorganisation of skin lesions Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-02-08 Cresle Andrei Zacarias, Rosimere Farias de Mendonça Florenziano, Thiago Antonio Moretti de Andrade, Andrea Aparecida de Aro, Maria Esméria Corezola do Amaral, Gláucia Maria Tech dos Santos, Marcelo Augusto Marretto Esquisatto
The aim of this study was to test the effect of electrical stimulation in association with topical Arnica montana gel on organisational changes in the dermis during tissue repair. An experimental rat incisional skin lesion was used for the study. This involved making an incisional lesion on the dorsum of the animals using a scalpel. Ninety-six animals were used divided into the following groups: control
-
The frequency of dysplastic branching crypts in colorectal polypoid tubular adenomas Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-02-03 Carlos A. Rubio, Michael Vieth, Corinna Lang-Schwarz
Dysplastic crypt branching (DCB) was recently found in ulcerative colitis-associated dysplasia. The aim was to assess the frequency and the branching phenotype of DCB in polypoid colorectal tubular adenomas (TA). A total of 3956 DCB were found in the 139 TA: 98% were in asymmetric branching (DCAB) and the remaining 2% in symmetric branching (DCSB). A linear correlation was found between DCB frequency
-
MIMT1 and LINC01550 are uncharted lncRNAs down-regulated in colorectal cancer Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-02-02 Faramarz Vejdandoust, Rahmaneh Moosavi, Nasrin Fattahi Dolatabadi, Atefeh Zamani, Hossein Tabatabaeian
Incomplete knowledge of the molecular basis of colorectal cancer, with subsequent limitations in early diagnosis and effective treatment, has contributed to this form of malignancy becoming the second most common cause of cancer-related death worldwide. With the advances in high-throughput profiling techniques and the availability of public data sets such as The Cancer Genome Atlas Program (TCGA),
-
Apoptosis versus necrosis in tubal ectopic pregnancies following Methotrexate Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-01-24 Yaron Gil, Asia Zubkov, Jacques Balayla, Aviad Cohen, Ishai Levin
Methotrexate administration for the treatment of tubal ectopic pregnancies has been shown to cause tubal mass enlargement. Our hypothesis was that, by administrating Methotrexate, a local necrotic reaction occurs, leading to hematoma formation and eventually fallopian tube rupture. Salpingectomy specimens were collected, analysed and divided into three equal groups: patients who received Methotrexate
-
Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2023-01-24 Charly Jehanno, Yann Le Page, Gilles Flouriot, Pascale Le Goff, Denis Michel
By depriving cancer cells of blood supplies of oxygen and nutrients, anti-angiogenic therapy is aimed at simultaneously asphyxiating and starving the cells. But in spite of its apparent logic, this strategy is generally counterproductive over the long term as the treatment seems to elicit malignancy. Since a defect of blood supply is expected to deprive tumours simultaneously of oxygen and nutrients
-
KLF13 overexpression protects sepsis-induced myocardial injury and LPS-induced inflammation and apoptosis Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-12-30 Ni Zeng, Zaijin Jian, Wenxin Zhu, Junmei Xu, Yongmei Fan, Feng Xiao
Sepsis remains a worldwide public health problem. This study aims to explore the role and mechanism of transcriptional factors (TFs) in sepsis-induced myocardial injury. Firstly, TF KLF13 was selected to explore its role in sepsis-induced myocardial injury. The caecal ligation and puncture (CLP) -induced sepsis mouse model was established and the septic mice were examined using standard histopathological
-
Silencing of RhoC induces macrophage M1 polarization to inhibit migration and invasion in colon cancer via regulating the PTEN/FOXO1 pathway Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-12-28 Bin Yang, Lihua Wang, Zhiying Tian
Ras homologue family member C (RhoC) is an oncogene in diverse types of human cancers, whereas its regulatory mechanisms involving macrophage polarization is rarely investigated. This study is designed to explore the regulatory role of RhoC in colon cancer and the underlying molecular mechanisms involving macrophage polarization. We detected RhoC expression by quantitative real-time polymerase chain
-
Expression of autocrine motility factor receptor (AMFR) in human breast and lung invasive micropapillary carcinomas Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-12-28 Jing Xu, Hongfei Ma, Qi Wang, Hui Zhang
The aim of this study was to evaluate the clinicopathological significance of autocrine motility factor receptor (AMFR) expression in a variety of human invasive micropapillary carcinomas (IMPC). AMFR expression was compared in 111 samples of a variety of human IMPCs which had intrinsic non-micropapillary components and with 26 cases of control pulmonary adenocarcinoma (CPA, carcinoma without an IMPC
-
Cilostazol attenuates oxidative stress and apoptosis in the quadriceps muscle of the dystrophic mouse experimental model Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-12-24 Túlio de Almeida Hermes, Rafael Dias Mâncio, Daniela Sayuri Mizobutti, Aline Barbosa Macedo, Larissa Akemi Kido, Valéria Helena Alves Cagnon Quitete, Elaine Minatel
Duchenne muscular dystrophy (DMD) is the most severe and frequent form of muscular dystrophy. The mdx mouse is one of the most widely used experimental models to understand aspects of the biology of dystrophic skeletal muscles and the mechanisms of DMD. Oxidative stress and apoptosis are present in early stages of the disease in mdx mice. The high production of reactive oxygen species (ROS) causes
-
Protection of dystrophic muscle cells using Idebenone correlates with the interplay between calcium, oxidative stress and inflammation Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-12-24 Amanda Harduim Valduga, Daniela Sayuri Mizobuti, Fernanda dos Santos Rapucci Moraes, Rafael Dias Mâncio, Luis Henrique Rapucci Moraes, Túlio de Almeida Hermes, Aline Barbosa Macedo, Elaine Minatel
There is strong cross-talk between abnormal intracellular calcium concentration, high levels of reactive oxygen species (ROS) and an exacerbated inflammatory process in the dystrophic muscles of mdx mice, the experimental model of Duchenne muscular dystrophy (DMD). In this study, we investigated effects of Idebenone, a potent anti-oxidant, on oxidative stress markers, the anti-oxidant defence system
-
British Society for Matrix Biology Spring 2022 Meeting. Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-12-01
-
Long-term intake of aspartame-induced cardiovascular toxicity is reflected in altered histochemical parameters, evokes oxidative stress, and trigger P53-dependent apoptosis in a mouse model Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-10-17 Hojat Anbara, Mehdi Kian, Gholam-Hossein Darya, Mohammad Taghi Sheibani
Aspartame (ASP) is probably the best known artificial sugar substitute that is used widely in food. Many experimental studies have reported the toxicity of long-term administration of ASP in various organ tissues. However, there is little evidence available about the nature and mechanisms of the adverse effects of long-term consumption of ASP on the cardiovascular system. This study was conducted to
-
Skin fibrosis associated with keloid, scleroderma and Jorge Lobo's disease (lacaziosis): An immuno-histochemical study Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-10-01 Wagner Luiz Tafuri, Thaise Yumie Tomokane, Ana Maria Gonçalves Silva, Luciane Kanashiro-Galo, David Miichael Mosser, Juarez Antonio Simões Quaresma, Carla Pagliari, Mirian N. Sotto
Fibrosis is a common pathophysiological response of many tissues and organs subjected to chronic injury. Despite the diverse aetiology of keloid, lacaziosis and localized scleroderma, the process of fibrosis is present in the pathogenesis of all of these three entities beyond other individual clinical and histological distinct characteristics. Fibrosis was studied in 20 samples each of these three
-
Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-09-24 Sachiko Imanishi, Shoko Nagata, Toshitsugu Fujita, Hodaka Fujii
Hepatocellular carcinoma (HCC) is the most predominant type of liver cancer and is frequently fatal. Alpha-fetoprotein, alpha-fetoprotein-L3, and protein induced by vitamin K absence or antagonist-II are used as biomarkers to diagnose HCC. However, these biomarkers are not highly specific, especially for early-stage HCC diagnosis; therefore, more specific biomarkers are needed. Recently, circular RNA
-
Morphophysiological alterations in fruit-eating bats after oral exposure to deltamethrin Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-09-04 Jerusa Maria Oliveira, Suellen Silva Condessa, Ana Luiza Fonseca Destro, Graziela Domingues Almeida Lima, Marli do Carmo Cupertino, Silvia Almeida Cardoso, Mariella Bontempo Freitas, Leandro Licursi de Oliveira
Deltamethrin (DTM) is a synthetic pyrethroid widely used in the cultivation and management of several crops due to its insecticidal action. Application to crops of pyrethroids such as DTM can result in the of water and fruit consumed by fruit bats having a high pyrethroid content which may be harmful. Therefore the objective of this study was to evaluate the effects of short-term oral exposure of the
-
Quantitative assessment of changes in skeletal muscle injury by computer-aided analysis based on two-dimensional ultrasonography combined with contrast-enhanced ultrasonography and estimated by a modified semi-quantitative scoring system: An experimental study in a contusion model Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-06-25 Jiaqi Zhao, Hejing Huang, Qi Xu, Qian Pan, Jia Guo
The aim of this study was to investigate the potential application of computer-aided analysis in the quantitative assessment of changes in skeletal muscle injury in the rabbit contusion model. Forty healthy rabbits were randomly divided into control (n = 5) and contusion (n = 35) groups. Rabbits in the contusion group were used to construct a muscle contusion model induced by a hammer hitting the right
-
MicroRNA-23a-3p promotes macrophage M1 polarization and aggravates lipopolysaccharide-induced acute lung injury by regulating PLK1/STAT1/STAT3 signalling Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-06-23 Tao Jiang, Li Sun, Jun Zhu, Ning Li, Haibo Gu, Ying Zhang, Miaomiao Li, Jiayao Xu
Macrophage polarization is an important effector process in acute lung injury (ALI) induced by sepsis. MicroRNAs (miRNAs) have emerged as important players in regulating ALI process. Here, we showed that elevated microRNA-23a-3p (miR-23a-3p) promoted LPS-induced macrophage polarization and ALI in mice, while inhibition of miR-23a-3p led to reduced macrophage response and ameliorated ALI inflammation
-
Histomorphometric and oxidative evaluation of the offspring's testis from type 2 diabetic female rats treated with metformin and pentoxifylline Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-06-23 Jessica Santana de Oliveira, Alluanan Adelson do Nascimento Silva, Fernanda Carolina Ribeiro Dias, Elizabeth Lopes de Oliveira, Emanuel Felipe de Oliveira Filho, Pierre Castro Soares, Catarina Michelle de Oliveira Ferreira, Valdemiro Amaro da Silva Junior
Type 2 diabetes mellitus (T2D) during pregnancy is characterized by high levels of reactive oxygen species and pro-inflammatory factors in the placenta. Once these reactive species reach the foetus, they trigger physiological adaptations that allow the foetus to survive, but programme the organism to develop metabolic disorders in adulthood. The male reproductive system is highly susceptible to foetal
-
Pre-analytical processing protocol of breast biopsies affects multigene panel results Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-05-15 Vanessa Reinaldo Lima, Beatriz da Costa Aguiar Alves, Fernando Luiz Affonso Fonseca, Debora Krutman Zveibil, Auro del Giglio
The creation of multigene panels for prognostic and predictive purposes allows a more accurate indication of adjuvant chemotherapy for patients with breast cancer. In a previous study, we reproduced a multigene panel of 21 genes based on the commercial Oncotype-DX method. We submitted 183 embedded specimens obtained from breast surgery on patients with locoregional disease (stages I to III) between
-
Expression of airway smooth muscle contractile proteins in children with acute interstitial pneumonia Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-05-08 Fang Cheng, Tao Lu, Yicheng Wang, Didi Yuan, Zehong Wei, Yongguo Li, Jianbo Li, Renkuan Tang
The purpose of the present study was to investigate the expression of α-SMA and SM22α in airway smooth muscle (ASM) of bronchioles from children younger than 14 years who died of acute interstitial pneumonia (AIP). This is based upon the hypothesis that as contractile marker proteins α-SMA and SM22α can serve as an index of the overcontractile phenotype of ASM that is seen in AIP. Lung tissue samples
-
Comparison of topical sucralfate with dexpanthenol in rat wound model Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-04-20 Eda Yildizhan, Burak Veli Ulger, Murat Akkus, Dilara Akinci, Omer Basol
Wound healing is a dynamic process initiated in response to injury. There are many factors that have detrimental effects on the wound healing process. Numerous studies have been conducted for improving wound healing processes. Dexpanthenol is widely used to accelerate wound healing. Sucralfate is used for the treatment of peptic ulcers. We aimed to compare the efficacy of topical Dexpanthenol and Sucralfate
-
Correlations between histological characterizations and methylation statuses of tumour suppressor genes in Wilms' tumours Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-04-18 Yen-Chein Lai, Meng-Yao Lu, Wen-Chung Wang, Tai-Cheng Hou, Chen-Yun Kuo
Wilms' tumour is a solid tumour that frequently occurs in children. Genetic changes in WT1 and epigenetic aberrations that affect imprinted control region 1 in WT2 loci are implicated in its aetiology. Moreover, tumour suppressor genes are frequently silenced by methylation in this tumour. In the present study, we analysed the methylation statuses of promoter regions of 24 tumour suppressor genes using
-
lncRNA MIAT targets miR-411-5p/STAT3/PD-L1 axis mediating hepatocellular carcinoma immune response Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-04-15 Xiaoxia Zhang, Banglun Pan, Jiacheng Qiu, Xiaoling Ke, Shuling Shen, Xiaoqian Wang, Nanhong Tang
Emerging evidences have shown that long noncoding RNA (lncRNA) plays an important role in the immune escape of cancer cells. Our previous study has demonstrated that lncRNA MIAT is associated with the immune infiltration of hepatocellular carcinoma (HCC). However, the underlying mechanism of MIAT regulating the PD-L1-mediated immune escape of HCC is poorly understood. Quantitative real-time PCR (qRT-PCR)
-
High level of FHL2 exacerbates the outcome of non-small cell lung cancer (NSCLC) patients and the malignant phenotype in NSCLC cells Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-04-02 Na Li, Ling Xu, Ji Zhang, Yongyu Liu
Non-small cell lung cancer (NSCLC) is a malignant tumour with high mortality. FHL2 has been identified as a biomarker of lung cancer. This research explored the effects of FHL2 expression on NSCLC. NSCLC-associated data sets were collected from the assistant for clinical bioinformatics and TCGA databases respectively. The association between FHL2 and clinical characteristics, the prognostic significance
-
Transient atrial inflammation in a murine model of Coxsackievirus B3-induced myocarditis Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-04-01 Linghe Wu, Mitchell D. Fiet, Daan R. Raaijmakers, Linde Woudstra, Albert C. van Rossum, Hans W. M. Niessen, Paul A. J. Krijnen
Atrial dysfunction is a relatively common complication of acute myocarditis, although its pathophysiology is unclear. There is limited information on myocarditis-associated histological changes in the atria and how they develop in time. The aim of this study therefore was to investigate inflammation, fibrosis and viral genome in the atria in time after mild CVB3-induced viral myocarditis (VM) in mice
-
Inflammatory response and immunohistochemical characterization of experimental calcium silicate-based perforation repair material Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-04-01 Hend Okasha, Ashraf M. Abu-Seida, Ahmed A. Hashem, Salma H. El Ashry, Mohamed M. Nagy
This study compares the immunohistochemical reaction of a new experimental tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA) and Biodentine. A total of 162 mature premolar teeth from 12 dogs were divided into three experimental groups (n = 54 teeth each) according to the evaluation period: 1, 2 and 3 months. Each group was further divided into two equal subgroups (n = 27
-
Autoantibodies directed against glutamate decarboxylase interfere with glucose-stimulated insulin secretion in dispersed rat islets Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-03-04 Varun Kamat, Jared R. Radtke, Qingxun Hu, Wang Wang, Ian R. Sweet, Christiane S. Hampe
Islet autoantibodies, including autoantibodies directed against the 65kDa isoform of glutamate decarboxylase (GAD65Ab), are present in the majority of patients with newly diagnosed type 1 diabetes (T1D). Whereas these autoantibodies are historically viewed as an epiphenomenon of the autoimmune response with no significant pathogenic function, we consider in this study the possibility that they impact
-
Investigation of the effect of metoclopramide on proliferation signal molecules in breast tissue Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-03-03 Nurcan Umur, Selda İldan Çalım, Gülce Naz Yazıcı, Seren Gulsen Gurgen
Metoclopramide (MCP) is a drug that has been widely used in recent years due to its hyperprolactinaemia effect on mothers during breastfeeding. The aim of this study was to investigate the proliferative changes that MCP may cause in the maternal breast tissue. In this study, 18 Wistar albino young–adult breastfeeding mothers with their offspring were divided into three groups: control group, low-dose
-
High-altitude hypoxia-induced rat alveolar cell injury by increasing autophagy Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-03-02 Zhen Zhao, Bin Hou, Li Tang, Yaping Wang, Yueqing Zhang, Zhanzhuan Ying, Jie Duo
Autophagy has been implicated in the pathogenesis of various lung diseases. This study aimed to investigate the role of autophagy in lung injury induced by high-altitude hypoxia. Wistar rats were randomized into four groups for exposure to normal altitude or high altitude for 1, 7, 14 and 21 days with no treatment or with the treatment of 1 mg/kg rapamycin or 2 mg/kg 3-methyladenine (3-MA) for consecutive
-
DNA methylation and miRNA expression in colon adenomas compared with matched normal colon mucosa and carcinomas Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-02-28 Mezgebe Gebrekiristos, Joshua Melson, Alice Jiang, Lela Buckingham
Dysregulation of DNA methylation patterns and non-coding RNA, including miRNAs, has been implicated in colon cancer, and these changes may occur early in the development of carcinoma. In this study, the role of epigenetics as early changes in colon tumorigenesis was examined through paired sample analysis of patient-matched normal, adenoma and carcinoma samples. Global methylation was assessed by genomic
-
Angiogenesis in patient-derived xenografts of odontogenic myxoma Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-02-28 Juliana Cristina de Souza, Victor Coutinho Bastos, Núbia Braga Pereira, Adriana Abalen Martins Dias, Gleide Fernandes de Avelar, Ricardo Santiago Gomez, Carolina Cavaliéri Gomes
Previously, by employing 3D organotypic tissue culture and patient-derived xenograft (PDX) model, oral myxoma response to a MAPK/MEK inhibitor was observed. Gross examination of the tumour fragments obtained after 55 days of PDX grafting revealed increased capsule vascularization. Microscopic analyses showed blood capillaries intermixed with myxoma cells, but the origin of these capillaries, from mice
-
Orchestrated expression of vasculogenic mimicry and laminin-5γ2 is an independent prognostic marker in oral squamous cell carcinoma Int. J. Exp. Pathol. (IF 3.0) Pub Date : 2022-02-16 Depanwita Saha, Debarpan Mitra, Neyaz Alam, Sagar Sen, Saunak Mitra Mustafi, Syamsundar Mandal, Biswanath Majumder, Nabendu Murmu
Vasculogenic mimicry (VM), an endothelial cell–independent alternative mechanism of blood supply to the malignant tumour, has long been considered as an adverse prognostic factor in many cancers. The correlation of VM with laminin-5γ2 and the assessment of their harmonized expression as an independent risk factor have not been elucidated yet in oral squamous cell carcinoma (OSCC). CD31/PAS staining