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Involvement of Fgf2-mediated tau protein phosphorylation in cognitive deficits induced by sevoflurane in aged rats Mol. Med. (IF 5.7) Pub Date : 2024-03-16 Xin Xie, Xiaomin Zhang, Songze Li, Wei Du
Anesthetics have been linked to cognitive alterations, particularly in the elderly. The current research delineates how Fibroblast Growth Factor 2 (Fgf2) modulates tau protein phosphorylation, contributing to cognitive impairments in aged rats upon sevoflurane administration. Rats aged 3, 12, and 18 months were subjected to a 2.5% sevoflurane exposure to form a neurotoxicity model. Cognitive performance
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Downregulation of HMGB1 carried by macrophage-derived extracellular vesicles delays atherosclerotic plaque formation through Caspase-11-dependent macrophage pyroptosis Mol. Med. (IF 5.7) Pub Date : 2024-03-16 Weijie Liang, Ruibin Wei, Xingxing Zhu, Jinliang Li, Aiwen Lin, Jun Chen, Wen Wu, Qiang Jie
Macrophage-derived extracellular vesicle (macrophage-EV) is highly studied for its regulatory role in atherosclerosis (AS). Our current study tried to elucidate the possible role of macrophage-EV loaded with small interfering RNA against high-mobility group box 1 (siHMGB1) affecting atherosclerotic plaque formation. In silico analysis was performed to find critical factors in mouse atherosclerotic
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A Journey in Science: Molecular vaccines for global child health in troubled times of anti-science Mol. Med. (IF 5.7) Pub Date : 2024-03-15 Peter J. Hotez
My scientific life in translational medicine runs in two parallel, yet often converging paths. The first, is four-decade-long commitment to develop new vaccines for parasitic and neglected tropical diseases, as well as pandemic threats. This includes a vaccine for human hookworm infection that I began as an MD-PhD student in New York City in the 1980s, and a new low-cost COVID vaccine that reached
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Huntingtin HTT1a is generated in a CAG repeat-length-dependent manner in human tissues Mol. Med. (IF 5.7) Pub Date : 2024-03-08 Franziska Hoschek, Julia Natan, Maximilian Wagner, Kirupa Sathasivam, Alshaimaa Abdelmoez, Björn von Einem, Gillian P. Bates, G. Bernhard Landwehrmeyer, Andreas Neueder
The disease-causing mutation in Huntington disease (HD) is a CAG trinucleotide expansion in the huntingtin (HTT) gene. The mutated CAG tract results in the production of a small RNA, HTT1a, coding for only exon 1 of HTT. HTT1a is generated by a block in the splicing reaction of HTT exon 1 to exon 2 followed by cleavage in intron 1 and polyadenylation. Translation of HTT1a leads to the expression of
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Artemisinin ameliorates cognitive decline by inhibiting hippocampal neuronal ferroptosis via Nrf2 activation in T2DM mice Mol. Med. (IF 5.7) Pub Date : 2024-03-07 Bo Wang, Sheng Zhu, Miao Guo, Run-Dong Ma, Ya-Ling Tang, Ya-Xiong Nie, Hong-Feng Gu
Neuronal ferroptosis plays a critical role in the pathogenesis of cognitive deficits. The present study explored whether artemisinin protected type 2 diabetes mellitus (T2DM) mice from cognitive impairments by attenuating neuronal ferroptosis in the hippocampal CA1 region. STZ-induced T2DM mice were treated with artemisinin (40 mg/kg, i.p.), or cotreated with artemisinin and Nrf2 inhibitor MEL385 or
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Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1 Mol. Med. (IF 5.7) Pub Date : 2024-03-06 Hengru Guo, Ying Xin, Saifei Wang, Xiaoning Zhang, Yanqi Ren, Bo Qiao, Hongjiang Li, Jing Wu, Xiao Hao, Lijun Xu, Yushan Yan, Haohao Zhang
Imbalance in energy regulation is a major cause of insulin resistance and diabetes. Melanocortin-4 receptor (MC4R) signaling at specific sites in the central nervous system has synergistic but non-overlapping functions. However, the mechanism by which MC4R in the arcuate nucleus (ARC) region regulates energy balance and insulin resistance remains unclear. The MC4Rflox/flox mice with proopiomelanocortin
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Serotonin as a biomarker of toxin-induced Parkinsonism Mol. Med. (IF 5.7) Pub Date : 2024-03-01 Anna Marie Buchanan, Sergio Mena, Iman Choukari, Aditya Vasa, Jesseca N. Crawford, Jim Fadel, Nick Maxwell, Lawrence Reagan, Allie Cruikshank, Janet Best, H. Fred Nijhout, Michael Reed, Parastoo Hashemi
Loss of dopaminergic neurons underlies the motor symptoms of Parkinson’s disease (PD). However stereotypical PD symptoms only manifest after approximately 80% of dopamine neurons have died making dopamine-related motor phenotypes unreliable markers of the earlier stages of the disease. There are other non-motor symptoms, such as depression, that may present decades before motor symptoms. Because serotonin
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USF1 transcriptionally activates USP14 to drive atherosclerosis by promoting EndMT through NLRC5/Smad2/3 axis Mol. Med. (IF 5.7) Pub Date : 2024-02-29 Zhiwen Zhang, Quan Guo, Chao Ma, Zhenzhou Zhao, Qingbo Shi, Haosen Yu, Lixin Rao, Muwei Li
Endothelial-to-Mesenchymal Transformation (EndMT) plays key roles in endothelial dysfunction during the pathological progression of atherosclerosis; however, its detailed mechanism remains unclear. Herein, we explored the biological function and mechanisms of upstream stimulating factor 1 (USF1) in EndMT during atherosclerosis. The in vivo and in vitro atherosclerotic models were established in high
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WSB1, as an E3 ligase, restrains myocardial ischemia–reperfusion injury by activating β-catenin signaling via promoting GSK3β ubiquitination Mol. Med. (IF 5.7) Pub Date : 2024-02-23 Lini Fang, Yang Tao, Guoying Che, Yongzi Yun, Min Ren, Yujie Liu
Reperfusion is the most effective strategy for myocardial infarct, but induces additional injury. WD repeat and SOCS box containing protein 1 (WSB1) plays a protective role in ischemic cells. This study aims to investigate the effects of WSB1 on myocardial ischemia–reperfusion (IR) injury. The myocardial IR was induced by left anterior descending (LAD) ligation for 45 min and subsequent reperfusion
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Role of ATF3 triggering M2 macrophage polarization to protect against the inflammatory injury of sepsis through ILF3/NEAT1 axis Mol. Med. (IF 5.7) Pub Date : 2024-02-23 Wei Wang, Rongli Xu, Ping He, Yuqing Xiong, Haomiao Zhao, Xuewei Fu, Jie Lin, Lijiao Ye
Sepsis is a systemic inflammatory response which is frequently associated with acute lung injury (ALI). Activating transcription factor 3 (ATF3) promotes M2 polarization, however, the biological effects of ATF3 on macrophage polarization in sepsis remain undefined. LPS-stimulated macrophages and a mouse model of cecal ligation and puncture (CLP)-induced sepsis were generated as in vitro and in vivo
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Understanding blaNDM-1 gene regulation in CRKP infections: toward novel antimicrobial strategies for hospital-acquired pneumonia Mol. Med. (IF 5.7) Pub Date : 2024-02-23 Liang Ding, Zheng Yang, Baier Sun
The escalating challenge of Carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital-acquired pneumonia (HAP) is closely linked to the blaNDM-1 gene. This study explores the regulatory mechanisms of blaNDM-1 expression and aims to enhance antibacterial tactics to counteract the spread and infection of resistant bacteria. KP and CRKP strains were isolated from HAP patients' blood samples. Transcriptomic
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G protein-coupled estrogen receptor activates PI3K/AKT/mTOR signaling to suppress ferroptosis via SREBP1/SCD1-mediated lipogenesis Mol. Med. (IF 5.7) Pub Date : 2024-02-21 Jiaping Chen, Rong Zhao, Yangwei Wang, Han Xiao, Wei Lin, Mingxin Diao, Shiwen He, Peiyuan Mei, Yongde Liao
Lung cancer is the leading cause of cancer-related death worldwide. The sex differences in the occurrence and fatality rates of non-small cell lung cancer (NSCLC), along with its association with estrogen dependence, suggest that estrogen receptors (ERs) contribute to the development of NSCLC. However, the influence of G protein-coupled estrogen receptor (GPER1) on NSCLC remains to be determined. Escape
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Exploring the therapeutic potential of isoorientin in the treatment of osteoporosis: a study using network pharmacology and experimental validation Mol. Med. (IF 5.7) Pub Date : 2024-02-20 Bo Zhang, Zechao Qu, Hua Hui, Baorong He, Dong Wang, Yong Zhang, Yiwei Zhao, Jingjun Zhang, Liang Yan
Isoorientin (ISO) is a glycosylated flavonoid with antitumor, anti-inflammatory, and antioxidant properties. However, its effects on bone metabolism remain largely unknown. In this study, we aimed to investigate the effects of ISO on receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast formation in vitro and bone loss in post-ovariectomy (OVX) rats, as well as to elucidate the
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Hypothesis of a CD137/Eomes activating axis for effector T cells in HPV oropharyngeal cancers Mol. Med. (IF 5.7) Pub Date : 2024-02-14 Robin Baudouin, Eric Tartour, Cécile Badoual, Stéphane Hans
Chronic Human Papilloma Virus (HPV) infection is supplanting alcohol and tobacco intoxications as the leading cause of oropharyngeal cancer in developed countries. HPV-related squamous cell carcinomas of the oropharynx (HPV + OSC) present better survival and respond better to radiotherapy and chemotherapy. Regulatory T cells (TREG) are mainly described as immunosuppressive and protumoral in most solid
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Scleral remodeling during myopia development in mice eyes: a potential role of thrombospondin-1 Mol. Med. (IF 5.7) Pub Date : 2024-02-14 Junhan Chen, Shin-ichi Ikeda, Yajing Yang, Yan Zhang, Ziyan Ma, Yifan Liang, Kazuno Negishi, Kazuo Tsubota, Toshihide Kurihara
Scleral extracellular matrix (ECM) remodeling plays a crucial role in the development of myopia, particularly in ocular axial elongation. Thrombospondin-1 (THBS1), also known as TSP-1, is a significant cellular protein involved in matrix remodeling in various tissues. However, the specific role of THBS1 in myopia development remains unclear. We employed the HumanNet database to predict genes related
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Porous Se@SiO2 nanospheres alleviate diabetic retinopathy by inhibiting excess lipid peroxidation and inflammation Mol. Med. (IF 5.7) Pub Date : 2024-02-06 Tian Niu, Xin Shi, Xijian Liu, Haiyan Wang, Kun Liu, Yupeng Xu
Lipid peroxidation is a characteristic metabolic manifestation of diabetic retinopathy (DR) that causes inflammation, eventually leading to severe retinal vascular abnormalities. Selenium (Se) can directly or indirectly scavenge intracellular free radicals. Due to the narrow distinction between Se’s effective and toxic doses, porous Se@SiO2 nanospheres have been developed to control the release of
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Fluvoxamine inhibits Th1 and Th17 polarization and function by repressing glycolysis to attenuate autoimmune progression in type 1 diabetes Mol. Med. (IF 5.7) Pub Date : 2024-02-05 Yuan Zou, Jing Zhang, Fei Sun, Qianqian Xu, Longmin Chen, Xi Luo, Ting Wang, Qing Zhou, Shu Zhang, Fei Xiong, Wen Kong, Ping Yang, Qilin Yu, Shiwei Liu, Cong-Yi Wang
Fluvoxamine is one of the selective serotonin reuptake inhibitors (SSRIs) that are regarded as the first-line drugs to manage mental disorders. It has been also recognized with the potential to treat inflammatory diseases and viral infection. However, the effect of fluvoxamine on autoimmune diseases, particularly type 1 diabetes (T1D) and the related cellular and molecular mechanisms, are yet to be
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M1 cholinergic signaling in the brain modulates cytokine levels and splenic cell sub-phenotypes following cecal ligation and puncture Mol. Med. (IF 5.7) Pub Date : 2024-02-05 Mabel N. Abraham, Ana Nedeljkovic-Kurepa, Tiago D. Fernandes, Omar Yaipen, Mariana R. Brewer, Daniel E. Leisman, Matthew D. Taylor, Clifford S. Deutschman
The contribution of the central nervous system to sepsis pathobiology is incompletely understood. In previous studies, administration of endotoxin to mice decreased activity of the vagus anti-inflammatory reflex. Treatment with the centrally-acting M1 muscarinic acetylcholine (ACh) receptor (M1AChR) attenuated this endotoxin-mediated change. We hypothesize that decreased M1AChR-mediated activity contributes
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Platelet-derived growth factor signaling in pericytes promotes hypothalamic inflammation and obesity Mol. Med. (IF 5.7) Pub Date : 2024-02-05 Akira Okekawa, Tsutomu Wada, Yasuhiro Onogi, Yuki Takeda, Yuichiro Miyazawa, Masakiyo Sasahara, Hiroshi Tsuneki, Toshiyasu Sasaoka
Pericytes are a vital component of the blood–brain barrier, and their involvement in acute inflammation was recently suggested. However, it remains unclear whether pericytes contribute to hypothalamic chronic inflammation and energy metabolism in obesity. The present study investigated the impact of pericytes on the pathophysiology of obesity by focusing on platelet-derived growth factor (PDGF) signaling
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Recent advances of NFATc1 in rheumatoid arthritis-related bone destruction: mechanisms and potential therapeutic targets Mol. Med. (IF 5.7) Pub Date : 2024-02-03 Hao Zheng, Yuexuan Liu, Yasi Deng, Yunzhe Li, Shiqi Liu, Yong Yang, Yun Qiu, Bin Li, Wenbing Sheng, Jinzhi Liu, Caiyun Peng, Wei Wang, Huanghe Yu
Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease characterized by inflammation of the synovial tissue and joint bone destruction, often leading to significant disability. The main pathological manifestation of joint deformity in RA patients is bone destruction, which occurs due to the differentiation and proliferation of osteoclasts. The transcription factor nuclear factor-activated
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A reliable transcriptomic risk-score applicable to formalin-fixed paraffin-embedded biopsies improves outcome prediction in localized prostate cancer Mol. Med. (IF 5.7) Pub Date : 2024-02-01 Michael Rade, Markus Kreuz, Angelika Borkowetz, Ulrich Sommer, Conny Blumert, Susanne Füssel, Catharina Bertram, Dennis Löffler, Dominik J. Otto, Livia A. Wöller, Carolin Schimmelpfennig, Ulrike Köhl, Ann-Cathrin Gottschling, Pia Hönscheid, Gustavo B. Baretton, Manfred Wirth, Christian Thomas, Friedemann Horn, Kristin Reiche
Clinical manifestation of prostate cancer (PCa) is highly variable. Aggressive tumors require radical treatment while clinically non-significant ones may be suitable for active surveillance. We previously developed the prognostic ProstaTrend RNA signature based on transcriptome‐wide microarray and RNA-sequencing (RNA-Seq) analyses, primarily of prostatectomy specimens. An RNA-Seq study of formalin-fixed
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Pretreatment with platelet-rich plasma protects against ischemia–reperfusion induced flap injury by deactivating the JAK/STAT pathway in mice Mol. Med. (IF 5.7) Pub Date : 2024-02-01 Linlin Su, Songtao Xie, Ting Li, Yanhui Jia, Yunchuan Wang
Ischemia–reperfusion (I/R) injury is a major cause of surgical skin flap compromise and organ dysfunction. Platelet-rich plasma (PRP) is an autologous product rich in growth factors, with tissue regenerative potential. PRP has shown promise in multiple I/R-induced tissue injuries, but its effects on skin flap injury remain unexplored. We evaluated the effects of PRP on I/R-injured skin flaps, optimal
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Extracellular CIRP induces CD4CD8αα intraepithelial lymphocyte cytotoxicity in sepsis Mol. Med. (IF 5.7) Pub Date : 2024-02-01 Yuichi Akama, Atsushi Murao, Monowar Aziz, Ping Wang
In sepsis, intestinal barrier dysfunction is often caused by the uncontrolled death of intestinal epithelial cells (IECs). CD4CD8αα intraepithelial lymphocytes (IELs), a subtype of CD4+ T cells residing within the intestinal epithelium, exert cytotoxicity by producing granzyme B (GrB) and perforin (Prf). Extracellular cold-inducible RNA-binding protein (eCIRP) is a recently identified alarmin which
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BATF and BATF3 deficiency alters CD8+ effector/exhausted T cells balance in skin transplantation Mol. Med. (IF 5.7) Pub Date : 2024-01-31 Chenghao Li, Zongtao Liu, Zihao Wang, Wai Yen Yim, Yajun Huang, Yuqi Chen
It is well-established that CD8+ T-cells play a critical role in graft rejection. The basic leucine zipper ATF-like transcription factor (BATF) and BATF3 are transcriptional factors expressed in T lymphocytes. Herein, we investigated the functions of BATF and BATF3 in the differentiation and exhaustion of CD8+ T cells following alloantigen activation. Wild-type CD8+ T cells, BATF-deficient (Batf−/−)
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α-Ketoglutarate improves cardiac insufficiency through NAD+-SIRT1 signaling-mediated mitophagy and ferroptosis in pressure overload-induced mice Mol. Med. (IF 5.7) Pub Date : 2024-01-22 Hao Yu, Daojing Gan, Zhen Luo, Qilin Yang, Dongqi An, Hao Zhang, Yingchun Hu, Zhuang Ma, Qingchun Zeng, Dingli Xu, Hao Ren
In heart failure (HF), mitochondrial dysfunction and metabolic remodeling lead to a reduction in energy productivity and aggravate cardiomyocyte injury. Supplementation with α-ketoglutarate (AKG) alleviated myocardial hypertrophy and fibrosis in mice with HF and improved cardiac insufficiency. However, the myocardial protective mechanism of AKG remains unclear. We verified the hypothesis that AKG improves
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Comprehensive analysis of m6A modification in lipopolysaccharide-induced acute lung injury in mice Mol. Med. (IF 5.7) Pub Date : 2024-01-22 Chenzhen Xu, Congkuan Song, Wenjie Wang, Bohao Liu, Guorui Li, Tinglv Fu, Bo Hao, Ning Li, Qing Geng
N6-Methyladenosine (m6A) methylation is the most prevalent post-transcriptional modification in mRNA, and plays significant roles in various diseases. Nevertheless, the precise functions of m6A modification in the formation of ALI remain unclear. In this study we explore the transcriptome distribution of m6A methylation and its probable roles of in ALI. Lipopolysaccharide (LPS) was utilized to establish
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Targeting the NAT10/NPM1 axis abrogates PD-L1 expression and improves the response to immune checkpoint blockade therapy Mol. Med. (IF 5.7) Pub Date : 2024-01-20 Ge Qin, Fan Bai, Huabin Hu, Jianwei Zhang, Weixiang Zhan, Zehua Wu, Jianxia Li, Yang Fu, Yanhong Deng
PD-1/PD-L1 play a crucial role as immune checkpoint inhibitors in various types of cancer. Although our previous study revealed that NPM1 was a novel transcriptional regulator of PD-L1 and stimulated the transcription of PD-L1, the underlying regulatory mechanism remains incompletely characterized. Various human cancer cell lines were used to validate the role of NPM1 in regulating the transcription
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Small noncoding RNAs and sperm nuclear basic proteins reflect the environmental impact on germ cells Mol. Med. (IF 5.7) Pub Date : 2024-01-20 Giulio Ferrero, Rosaria Festa, Laura Follia, Gennaro Lettieri, Sonia Tarallo, Tiziana Notari, Antonella Giarra, Carmela Marinaro, Barbara Pardini, Alessandra Marano, Giulia Piaggeschi, Carla Di Battista, Marco Trifuoggi, Marina Piscopo, Luigi Montano, Alessio Naccarati
Molecular techniques can complement conventional spermiogram analyses to provide new information on the fertilizing potential of spermatozoa and to identify early alterations due to environmental pollution. Here, we present a multilevel molecular profiling by small RNA sequencing and sperm nuclear basic protein analysis of male germ cells from 33 healthy young subjects residing in low and high-polluted
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Diabetes-induced male infertility: potential mechanisms and treatment options Mol. Med. (IF 5.7) Pub Date : 2024-01-15 Runchun Huang, Jiawang Chen, Buyu Guo, Chenjun Jiang, Weiming Sun
Male infertility is a physiological phenomenon in which a man is unable to impregnate a fertile woman during a 12-month period of continuous, unprotected sexual intercourse. A growing body of clinical and epidemiological evidence indicates that the increasing incidence of male reproductive problems, especially infertility, shows a very similar trend to the incidence of diabetes within the same age
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Age-related decline in melatonin contributes to enhanced osteoclastogenesis via disruption of redox homeostasis Mol. Med. (IF 5.7) Pub Date : 2024-01-12 Di-Zheng Wu, Guo-Zheng Zhu, Kai Zhao, Jia-Wen Gao, Gui-Xing Cai, Hong-Zhou Li, Yu-Sheng Huang, Chen Tu, Jing-Shen Zhuang, Zhi-Wei Huang, Zhao-Ming Zhong
Increased oxidative stress contributes to enhanced osteoclastogenesis and age-related bone loss. Melatonin (MT) is an endogenous antioxidant and declines with aging. However, it was unclear whether the decline of MT was involved in the enhanced osteoclastogenesis during the aging process. The plasma level of MT, oxidative stress status, bone mass, the number of bone marrow-derived monocytes (BMMs)
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Lysine demethylase 5C inhibits transcription of prefoldin subunit 5 to activate c-Myc signal transduction and colorectal cancer progression Mol. Med. (IF 5.7) Pub Date : 2024-01-12 Fulong Yu, Liang Li, Yimei Gu, Song Wang, Lianbang Zhou, Xiaohu Cheng, Heng Jiang, Yang Huang, Yingfeng Zhang, Wenbao Qian, Xianghua Li, Zhining Liu
Lysine demethylase 5C (KDM5C) has been implicated in the development of several human cancers. This study aims to investigate the role of KDM5C in the progression of colorectal cancer (CRC) and explore the associated molecular mechanism. Bioinformatics tools were employed to predict the target genes of KDM5C in CRC. The expression levels of KDM5C and prefoldin subunit 5 (PFDN5) in CRC cells were determined
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PRMT3 methylates HIF-1α to enhance the vascular calcification induced by chronic kidney disease Mol. Med. (IF 5.7) Pub Date : 2024-01-10 Guangyu Zhou, Chen Zhang, Hui Peng, Xuesong Su, Qun Huang, Zixia Zhao, Guangyi Zhao
Medial vascular calcification is commonly identified in chronic kidney disease (CKD) patients and seriously affects the health and life quality of patients. This study aimed to investigate the effects of protein arginine methyltransferase 3 (PRMT3) on vascular calcification induced by CKD. A mice model of CKD was established with a two-step diet containing high levels of calcium and phosphorus. Vascular
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Decellularized nucleus pulposus matrix/chitosan hybrid hydrogel combined with nucleus pulposus stem cells and GDF5-loaded microspheres for intervertebral disc degeneration prevention Mol. Med. (IF 5.7) Pub Date : 2024-01-10 Tao Ma, Chen Liu, Quanlai Zhao, Yu Zhang, Liang Xiao
Intervertebral disc degeneration (IDD) is considered an important pathological basis for spinal degenerative diseases. Tissue engineering is a powerful therapeutic strategy that can effectively restore the normal biological properties of disc units. In this study, hydrogels loaded with growth/differentiation factor 5 (GDF5) and stem cells were combined to provide an effective strategy for nucleus pulposus
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IRF4 affects the protective effect of regulatory T cells on the pulmonary vasculature of a bronchopulmonary dysplasia mouse model by regulating FOXP3 Mol. Med. (IF 5.7) Pub Date : 2024-01-09 Ying Zhu, Langyue He, Yue Zhu, Huici Yao, Jianfeng Jiang, Hongyan Lu
Bronchopulmonary dysplasia (BPD) is a common chronic lung disease in preterm infants, characterised by compromised alveolar development and pulmonary vascular abnormalities. Emerging evidence suggests that regulatory T cells (Tregs) may confer protective effects on the vasculature. Knockdown of their transcription factor, interferon regulatory factor 4 (IRF4), has been shown to promote vascular endothelial
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Soy isoflavones induces mitophagy to inhibit the progression of osteosarcoma by blocking the AKT/mTOR signaling pathway Mol. Med. (IF 5.7) Pub Date : 2024-01-08 Ziang Zheng, Xinghan Zhao, Bo Yuan, Shan Jiang, Rushan Yan, Xiaowei Dong, Qijun Yao, Haidong Liang
Soy isoflavones (SI) is a natural bioactive substance exhibiting beneficial effects on human health. This study aims to elucidate the therapeutic potential of SI in the treatment of osteosarcoma (OS) and to investigate the underlying mechanisms, particularly focusing on mitophagy. The effects of SI on the proliferation, apoptosis, migration, and invasion of U2OS cells were analyzed. Mitophagy was assessed
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Key roles of autophagosome/endosome maturation mediated by Syntaxin17 in methamphetamine-induced neuronal damage in mice Mol. Med. (IF 5.7) Pub Date : 2024-01-03 Xi Wang, Miaoyang Hu, Jingrong Chen, Xinyu Lou, Hongchao Zhang, Muhan Li, Jie Cheng, Tengfei Ma, Jianping Xiong, Rong Gao, Xufeng Chen, Jun Wang
Autophagic defects are involved in Methamphetamine (Meth)-induced neurotoxicity. Syntaxin 17 (Stx17), a member of the SNARE protein family, participating in several stages of autophagy, including autophagosome-late endosome/lysosome fusion. However, the role of Stx17 and potential mechanisms in autophagic defects induced by Meth remain poorly understood. To address the mechanism of Meth-induced cognitive
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A new physiological medium uncovers biochemical and cellular alterations in Lesch-Nyhan disease fibroblasts Mol. Med. (IF 5.7) Pub Date : 2024-01-03 Paula Escudero-Ferruz, Neus Ontiveros, Claudia Cano-Estrada, Diane J. Sutcliffe, H. A. Jinnah, Rosa J. Torres, José M. López
Lesch-Nyhan disease (LND) is a severe neurological disorder caused by the genetic deficiency of hypoxanthine–guanine phosphoribosyltransferase (HGprt), an enzyme involved in the salvage synthesis of purines. To compensate this deficiency, there is an acceleration of the de novo purine biosynthetic pathway. Most studies have failed to find any consistent abnormalities of purine nucleotides in cultured
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Cord blood-derived biologics lead to robust axonal regeneration in benzalkonium chloride-injured mouse corneas by modulating the Il-17 pathway and neuropeptide Y Mol. Med. (IF 5.7) Pub Date : 2024-01-03 Ruojing Huang, Caiying Su, Na Zhang, Congying Shi, Guangming Pu, Yong Ding, Wei Wei, Jiansu Chen
Umbilical cord blood-derived therapeutics, such as serum (UCS) and platelet-rich plasma (UCPRP), are popular treatment options in clinical trials and can potentially be utilized to address a clinically unmet need caused by preservatives, specifically benzalkonium chloride (BAK), present in ophthalmic formulations. As current clinical interventions for secondary injuries caused by BAK are suboptimal
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The potential role of ocular and otolaryngological mucus proteins in myalgic encephalomyelitis/chronic fatigue syndrome Mol. Med. (IF 5.7) Pub Date : 2024-01-03 Kaylin Huitsing, Tara Tritsch, Francisco Javier Carrera Arias, Fanny Collado, Kristina K. Aenlle, Lubov Nathason, Mary Ann Fletcher, Nancy G. Klimas, Travis J. A. Craddock
Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a debilitating illness associated with a constellation of other symptoms. While the most common symptom is unrelenting fatigue, many individuals also report suffering from rhinitis, dry eyes and a sore throat. Mucin proteins are responsible for contributing to the formation of mucosal membranes throughout the body. These mucosal pathways
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ALDH2 mitigates LPS-induced cardiac dysfunction, inflammation, and apoptosis through the cGAS/STING pathway Mol. Med. (IF 5.7) Pub Date : 2023-12-20 Haoran Liu, Qin Hu, Ke Ren, Pengxin Wu, Yang Wang, Chuanzhu Lv
Sepsis is a severe syndrome of organ dysfunction that often leads to cardiac dysfunction and endangers life. The role of mitochondrial aldehyde dehydrogenase 2 (ALDH2) in LPS-induced myocardial injury is unclear. The purpose of this study was to assess the role of ALDH2 in lipopolysaccharide (LPS)-induced myocardial injury and the regulatory mechanism and to identify potential therapeutic strategies
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TIM-3 regulates the proliferation by BDNF-mediated PI3K/AKT axis in the process of endometriosis Mol. Med. (IF 5.7) Pub Date : 2023-12-19 Wei Tian, Min Liu, Yuqiu Liu, Qingfeng Lv, HuaFeng Cheng, Yanling Gu, Mingjiang Li
T cell immunoglobulin and mucin domain-containing molecule-3 (TIM-3) initially discovered on the surface of Th1 cells, negatively regulates immune responses and mediates apoptosis of Th1 cells. An increasing number of studies have since shown that TIM-3 is crucial in the genesis and development of immune diseases, cancers, and chronic infectious illnesses. However, the effect of TIM-3 on endometriosis
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Cyclosporine A alleviates colitis by inhibiting the formation of neutrophil extracellular traps via the regulating pentose phosphate pathway Mol. Med. (IF 5.7) Pub Date : 2023-12-13 Chenjing Xu, Ziping Ye, Wenyu Jiang, Shu Wang, Hongjie Zhang
The aberrant formation of neutrophil extracellular traps (NETs) has been implicated in ulcerative colitis (UC), a chronic recurrent intestinal inflammation. Cyclosporine A (CsA) is now applied as rescue therapy for acute severe UC. In addition, it has been certained that CsA inhibits the formation of NETs in vitro and the mechanism of which was still vague. The study aimed to explore the mechanism
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Efficacy and mechanism of Shenqi Compound in inhibiting diabetic vascular calcification Mol. Med. (IF 5.7) Pub Date : 2023-12-13 Chan Yang, Ziyan Xie, Hanyu Liu, Xueru Wang, Zehua Zhang, Lian Du, Chunguang Xie
Shenqi Compound (SQC) has been used in clinic for several decades in the prevention and treatment of diabetes and its complications. But this is merely a heritage of experience. The primary aim of this study is to scientifically validate the therapeutic effects of SQC on diabetic vascular calcification (DVC) in an animal model and, simultaneously, uncover its potential underlying mechanisms. Spontaneous
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Glabridin improves autoimmune disease in Trex1-deficient mice by reducing type I interferon production Mol. Med. (IF 5.7) Pub Date : 2023-12-08 Jincai Wen, Wenqing Mu, Hui Li, Yulu Yan, Xiaoyan Zhan, Wei Luo, Zhongxia Wang, Wen Kan, Jia Zhao, Siwen Hui, Ping He, Shuanglin Qin, Yingjie Xu, Ping Zhang, Xiaohe Xiao, Guang Xu, Zhaofang Bai
The cGAS-STING signaling pathway is an essential section of the natural immune system. In recent years, an increasing number of studies have shown a strong link between abnormal activation of the cGAS-STING signaling pathway, a natural immune pathway mediated by the nucleic acid receptor cGAS, and the development and progression of autoimmune diseases. Therefore, it is important to identify an effective
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The role of N-methyladenosine modification in acute and chronic kidney diseases Mol. Med. (IF 5.7) Pub Date : 2023-12-08 Saiqi Qi, Jie Song, Linjun Chen, Huachun Weng
N6-methyladenosine (m6A) modification is a kind of RNA modification in which methylation occurs at the sixth N position in adenosine in RNA, which can occur in various RNAs such as mRNAs, lncRNAs and miRNAs. This is one of the most prominent and frequent posttranscriptional modifications within organisms and has been shown to function dynamically and reversibly in a variety of ways, including splicing
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FGF21 alleviates endothelial mitochondrial damage and prevents BBB from disruption after intracranial hemorrhage through a mechanism involving SIRT6 Mol. Med. (IF 5.7) Pub Date : 2023-12-04 Runfeng Wang, Jin Wang, Zhiguo Zhang, Bo Ma, Shukai Sun, Li Gao, Guodong Gao
Disruption of the BBB is a harmful event after intracranial hemorrhage (ICH), and this disruption contributes to a series of secondary injuries. We hypothesized that FGF21 may have protective effects after intracranial hemorrhage (ICH) and investigated possible underlying molecular mechanisms. Blood samples of ICH patients were collected to determine the relationship between the serum level of FGF21
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β-aminoisobutyrics acid, a metabolite of BCAA, activates the AMPK/Nrf-2 pathway to prevent ferroptosis and ameliorates lung ischemia-reperfusion injury Mol. Med. (IF 5.7) Pub Date : 2023-12-04 Ziyue Zhang, Xingbing Li, Jingwen Guo, Bo He, Lianpan Wu, Rongpei Yang, Xingyue Li, Dandong Fang, XiaoLi Yang, Donghai Yang, Fengxian Wang, Ming Tang, Yu Han, Pedro A. Jose, Hongyong Wang, Chunyu Zeng
Lung ischemia-reperfusion (I/R) injury is a serious clinical problem without effective treatment. Enhancing branched-chain amino acids (BCAA) metabolism can protect against cardiac I/R injury, which may be related to bioactive molecules generated by BCAA metabolites. L-β-aminoisobutyric acid (L-BAIBA), a metabolite of BCAA, has multi-organ protective effects, but whether it protects against lung I/R
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Sustained activation of 12/15 lipoxygenase (12/15 LOX) contributes to impaired renal recovery post ischemic injury in male SHR compared to females Mol. Med. (IF 5.7) Pub Date : 2023-12-04 Riyaz Mohamed, Jennifer C. Sullivan
Acute kidney injury (AKI) due to ischemia-reperfusion (IR) is a serious and frequent complication in clinical settings, and mortality rates remain high. There are well established sex differences in renal IR, with males exhibiting greater injury following an ischemic insult compared to females. We recently reported that males have impaired renal recovery from ischemic injury vs. females. However, the
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The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression Mol. Med. (IF 5.7) Pub Date : 2023-12-01 Wanru Ma, Junhua Hu
The linear long non-coding RNA P14AS has previously been reported to be dysregulated in colon cancer, but the mechanistic role that P14AS plays in colon cancer progression has yet to be clarified. Accordingly, this study was developed to explore the regulatory functions of ANRIL linear transcript-P14AS in cancer. The expression of P14AS, ANRIL, miR-23a-5p and their target genes were detected by quantitative
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IGF2 deficiency promotes liver aging through mitochondrial dysfunction and upregulated CEBPB signaling in d-galactose-induced aging mice Mol. Med. (IF 5.7) Pub Date : 2023-11-28 Xiaohai Zhou, Bowen Tan, Weiwei Gui, Caiping Zhou, Hanxin Zhao, Xihua Lin, Hong Li
Liver aging, marked by cellular senescence and low-grade inflammation, heightens susceptibility to chronic liver disease and worsens its prognosis. Insulin-like growth factor 2 (IGF2) has been implicated in numerous aging-related diseases. Nevertheless, its role and underlying molecular mechanisms in liver aging remain largely unexplored. The expression of IGF2 was examined in the liver of young (2–4 months)
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TET2 deficiency promotes anxiety and depression-like behaviors by activating NLRP3/IL-1β pathway in microglia of allergic rhinitis mice Mol. Med. (IF 5.7) Pub Date : 2023-11-27 Ziang Gao, Hao Lv, Yunfei Wang, Yulie Xie, Mengting Guan, Yu Xu
Anxiety and depression-like behaviors in allergic rhinitis (AR) are attracting attention, while the precise mechanism has not been clearly elucidated. Recent evidence shows that neuroinflammation in anterior cingulate cortex (ACC) may be the core of these neuropsychiatric symptoms in AR. Here, we investigated the molecular link between the anxiety and depression-like behaviors and neuroinflammation
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Role of DCLK1/Hippo pathway in type II alveolar epithelial cells differentiation in acute respiratory distress syndrome Mol. Med. (IF 5.7) Pub Date : 2023-11-23 Xiao-Yue Chen, Ching Kao, Syue-Wei Peng, Jer-Hwa Chang, Yueh-Lun Lee, Vincent Laiman, Kian Fan Chung, Pankaj K. Bhavsar, Didik Setyo Heriyanto, Kai-Jen Chuang, Hsiao-Chi Chuang
Delay in type II alveolar epithelial cell (AECII) regeneration has been linked to higher mortality in patients with acute respiratory distress syndrome (ARDS). However, the interaction between Doublecortin-like kinase 1 (DCLK1) and the Hippo signaling pathway in ARDS-associated AECII differentiation remains unclear. Therefore, the objective of this study was to understand the role of the DCLK1/Hippo
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Maresin1 ameliorates MSU crystal-induced inflammation by upregulating Prdx5 expression Mol. Med. (IF 5.7) Pub Date : 2023-11-23 Hui Jiang, DianZe Song, Xiaoqin Zhou, Feng Chen, Qingqing Yu, Long Ren, Qian Dai, Mei Zeng
Maresin1 (MaR1) is a potent lipid mediator that exhibits significant anti-inflammatory activity in the context of several inflammatory diseases. A previous study reported that MaR1 could suppress MSU crystal-induced peritonitis in mice. To date, the molecular mechanism by which MaR1 inhibits MSU crystal-induced inflammation remains poorly understood. Mousebone marrow-derived macrophages (BMDMs) were
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USP38 exacerbates atrial inflammation, fibrosis, and susceptibility to atrial fibrillation after myocardial infarction in mice Mol. Med. (IF 5.7) Pub Date : 2023-11-12 Yang Gong, Tingting Yu, Wei Shuai, Tao Chen, Jingjing Zhang, He Huang
Inflammation plays an important role in the pathogenesis of atrial fibrillation (AF) after myocardial infarction (MI). The role of USP38, a member of the ubiquitin-specific protease family, on MI-induced atrial inflammation, fibrosis, and associated AF is unclear. In this study, we surgically constructed a mouse MI model using USP38 cardiac conditional knockout (USP38-CKO) and cardiac-specific overexpression
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Loss of Krüppel-like factor-10 facilitates the development of chemical-induced liver cancer in mice Mol. Med. (IF 5.7) Pub Date : 2023-11-09 Sung Hwan Yoo, Ji Hae Nahm, Woon Kyu Lee, Hyun Woong Lee, Hye Young Chang, Jung Il Lee
Krüppel-like factor 10 (KLF10) is involved in a positive feedback loop that regulates transforming growth factor β (TGFβ) signaling, and TGFβ plays an important role in the pathogenesis of liver disease. Here, we investigated whether KLF10 deletion affects the development of liver fibrosis and hepatocellular carcinoma (HCC). We induced KLF10 deletion in C57BL/6 mice. Liver fibrosis was induced by feeding
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Exosomal circCOL1A1 promotes angiogenesis via recruiting EIF4A3 protein and activating Smad2/3 pathway in colorectal cancer Mol. Med. (IF 5.7) Pub Date : 2023-11-08 Gui Hu, Changwei Lin, Kai Gao, Miao Chen, Fei Long, Buning Tian
Colorectal cancer (CRC) is the third frequently diagnosed cancer with high incidence and mortality rate worldwide. Our previous report has demonstrated that circCOL1A1 (hsa_circ_0044556) functions as an oncogene in CRC, and Gene Ontology (GO) analysis has also revealed the strong association between circCOL1A1 and angiogenesis. However, the mechanism of circCOL1A1 or exosomal circCOL1A1 in CRC angiogenesis
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PP121, a dual inhibitor of tyrosine and phosphoinositide kinases, relieves airway hyperresponsiveness, mucus hypersecretion and inflammation in a murine asthma model Mol. Med. (IF 5.7) Pub Date : 2023-11-07 Wei Li, Lu Xue, Changsi Peng, Ping Zhao, Yongbo Peng, Weiwei Chen, Wenyi Wang, Jinhua Shen
Tyrosine kinase and phosphoinositide kinase pathways play important roles in asthma formation. As a dual tyrosine and phosphoinositide kinase inhibitor, PP121 has shown anticancer efficacy in multiple tumors. However, the study of PP121 in pulmonary diseases is still limited. Herein, we investigated the therapeutic activities of PP121 in asthma treatment. Tension measurements and patch clamp recordings
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Performance of the MeltPro TB assay as initial test for diagnosis of pulmonary tuberculosis with drug-resistance detection Mol. Med. (IF 5.7) Pub Date : 2023-11-07 Zhi-bin Liu, Li-ping Cheng, Hong-qiu Pan, Xiao-cui Wu, Fu-hui Lu, Jie Cao, Lei Wang, Wei Wei, Hong-yu Chen, Wei Sha, Qin Sun
The MeltPro TB assay (MeltPro) is a molecular rapid diagnostic test designed for detecting resistance to antituberculosis drugs. However, the performance of MeltPro as an initial diagnostic test for simultaneously detecting the presence of Mycobacterium tuberculosis (MTB) and drug resistance has not been evaluated. This study aims to assess the performance of MeltPro as initial diagnostic test for
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Sufficient Cav-1 levels in the endothelium are critical for the maintenance of the neurovascular unit in the retina Mol. Med. (IF 5.7) Pub Date : 2023-11-03 Yixin Wang, Mahmoud Halawa, Anupriya Chatterjee, Rachana Eshwaran, Yi Qiu, Yohanes Cakrapradipta Wibowo, Jianyuan Pan, Thomas Wieland, Yuxi Feng
Caveolin-1 (Cav-1) is a pivotal protein in the plasma membrane. Studies on homozygous Cav-1 deficient mice revealed that Cav-1 is essential for endothelial function and angiogenesis in the retina. However, whether a reduction in Cav-1 content hampers the neurovascular unit (NVU) in the retina is unclear. Thus, this study examines the NVU in the retinas of heterozygous Cav-1 deficient (Cav-1+/−) mice
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5α-Hydroxycostic acid inhibits choroidal neovascularization in rats through a dual signalling pathway mediated by VEGF and angiopoietin 2 Mol. Med. (IF 5.7) Pub Date : 2023-11-01 Wulong Lei, Huan Xu, Hao Yao, Lanjiao Li, Menglei Wang, Xiyuan Zhou, Xueqin Liu
5α-Hydroxycostic acid is a eudemane sesquiterpene that is isolated from the natural plant, Laggera alata. It exerts anti-inflammatory and anti-angiogenic effects on human breast cancer cells, but its role and underlying mechanism in choroidal neovascularization (CNV) are still unclear. We conducted a study to verify that 5α-Hydroxycostic acid can inhibit the formation and leakage of CNV, and describe