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  • Photoactivatable Fluorescent Probes for Spatiotemporal-Controlled Biosensing and Imaging
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-11
    Zhen Zou; Ziling Luo; Xuan Xu; Sheng Yang; Zhihe Qing; Juewen Liu; Ronghua Yang

    Designing fluorescent probes for accurately analyzing target molecules of interest become fascinating and vital in the view of biology and biomedicine. Conventional fluorescent probes are often obscured by the interference from biological autofluorescence, off-target effects, and a lack of spatiotemporal control. As a newly developed paradigm, photoactivatable fluorescent probes (PFPs), whose sensing or imaging behaviors can be remotely activated by light, provide great potentials in biochemical information acquisition with high spatial and temporal resolution. Over the past decades, substantial progress has been made in designing PFPs for sensing and imaging of biologically active molecules. In this review, we display an overview of current representative strategies and their underlying mechanisms, including light-inducible target recognition, light-controlled enzymatic activity, and photoactivatable fluorophores. Finally, the challenges and prospects in this promising field are featured on the basis of its current development.

  • Biopolymers in sorbent-based microextraction methods
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-10
    Idaira Pacheco-Fernández; Diego W. Allgaier-Díaz; Giulia Mastellone; Cecilia Cagliero; David Díaz Díaz; Verónica Pino
  • Emerging Vistas on Electrochemical Detection of Diabetic Retinopathy Biomarkers
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-08
    K.S. Shalini Devi; Madhurantakam Sasya; Uma Maheswari Krishnan

    Diabetes is a widely prevalent condition that is accompanied by additional metabolic dysfunctions thereby predisposing the individual to several other complications. One such complication that results in vision impairment is diabetic retinopathy (DR). Monitoring glucose levels alone is not sufficient for predicting the risk of DR in an individual. Though several markers have been identified for predicting the risk of DR, the diagnostic methods have remained confined to traditional spectroscopy and immunoassays that are expensive, time-consuming and in many cases, lack specificity. It is in this context, portable and rapid detection strategies assume significance with respect to clinical diagnosis. Electrochemical sensors offer several advantages of miniaturization, rapid response, and high specificity. These attributes when combined with the additional advantages of nanotechnology offer promise for the detection of DR biomarkers with high precision and speed. This review summarizes some of the major biomarkers of DR and the different types of electrochemical detection strategies designed for their quantification in past two decades.

  • Surface-enhanced Raman spectroscopy for polychlorinated biphenyl detection: Recent developments and Future Prospects
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-06
    Jie Cheng; Peilong Wang; Xiao-Ou Su

    Polychlorinated biphenyls (PCBs) have gained much attention for their carcinogenic, teratogenic, and mutagenic properties. Although banned in the 1970s, PCBs are still frequently found worldwide in environment, animal-origin food and natural waters. Therefore, the development of determination methods is an indispensable step for the monitoring of PCBs. Surface-enhanced Raman spectroscopy (SERS) is an emerging technique for the chemical analysis. With the advantages of excellent sensitivity and significant enhancement to identify the target, SERS has become a promising alternative method for PCBs analysis. This paper comprehensively reviews the recent progress of SERS development in the determination of PCBs, mainly focusing on the preparation of SERS substrates. As the current SERS research on PCBs analysis is still in an early stage, there are several hurdles for further advancing SERS for complex samples. This review includes our discussion on the current challenges and outlook on real-word applications of SERS in PCBs analysis.

  • Peptidomic Analyses: The progress in enrichment and identification of endogenous peptides
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-06
    Jiaxi Peng; Hongyan Zhang; Huan Niu; Ren’an Wu

    Peptidomics is the comprehensive profiling of endogenous peptides from the biological sources. As with genome, transcriptome, proteome and metabolome, the peptidome is directly related with individuals’ physiological state at the peptide level, which can be one of the most promising sources of pathophysiological information. Thus a comprehensive understanding of peptidome is very meaningful for clinical applications. However, low-abundance endogenous peptides present in highly complex biological environments with large heterogeneity in sizes and charges, making peptidome studies challenging. In order to clarify the peptidome in biological samples, numerous technologies and analytical methods in peptidomics have been developed which greatly promote the subject going forward. In this review, recent advances on technologies and methods developed for peptidome research are summarized with emphasis on the ingeniously designed adsorbents for peptide extract, MS technologies for peptide characterization, and data mining for function elucidation. Existing challenges and future perspectives in peptidomics are discussed.

  • Nanomaterials for the detection of halides and halogen oxyanions by colorimetric and luminescent techniques: a critical overview
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-06
    Francisco Pena-Pereira; Adrián García-Figueroa; Isela Lavilla; Carlos Bendicho

    The increasing interest in halides and halogen oxyanions monitoring has led to a strong demand of sensitive and selective analytical methodologies for their determination. In this sense, the implementation of nanostructures in analytical systems has been profusely explored in recent years for optical sensing of halides and halogen oxyanions in samples of varying complexity. The present work provides a critical overview of contributions devoted to colorimetric and luminescent determination of relevant halogen-containing species by taking advantage of the unique properties of NPs. Particular attention has been paid to the different strategies followed for optical sensing of target analytes. In addition, analytical validation of reported methods is critically evaluated and guidance for compliance with validation requirements is provided.

  • The role of nanomaterials on the cancer cells sensing based on folate receptor: Analytical approach
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-06
    Jafar Soleymani; Mohammad Hasanzadeh; Nasrin shadjou; Mohammad Hossein Somi; Abolghasem Jouyban
  • Recent advances of ionic liquids in sample preparation
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-04
    Juanjuan Feng; Herman Maloko Loussala; Sen Han; Xiangping Ji; Chunying Li; Min Sun

    This paper reviews the recent advances of ionic liquids (ILs) in sample preparation from more than 100 works mainly in last five years, including liquid-liquid microextraction, solid-phase extraction, solid-phase microextraction, stir bar or stir cake sorptive extraction. ILs, polymer of ILs and their composites with molecular imprinted polymer, carbon nanotubes, graphene oxide and magnetic materials have been explored as promising sorbents for inorganic or organic pollutants, majorly relying on the affinity of ILs with the target. The improved sensitivity and selectivity towards different types of analytes were displayed, attributed from various mechanism of ILs-based extraction materials including π-π, ion-exchange, hydrogen bond, dipole-dipole, electrostatic, hydrophobic and hydrophilic interactions. The prospects of ILs for sample preparation were also presented, which can offer an outlook for the further applications of ILs.

  • Chiral analysis by nano-liquid chromatography
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-02-03
    G. D’Orazio

    The concept of stereoisomerism in the biological activity of compounds in living systems emerged in 1948 with the work of Louis Pasteur, who first observed the optical activity of tartaric acid. Enantiomers can produce undesired effects on biological system, due to their different optical activity, even though they possess the same physico-chemical properties in an achiral environment. For this reason, they are a topic that has arisen interest in different areas including pharmaceutical, biomedical, agrochemical and food fields etc. Nowadays, the development of arduous stereospecific synthesis for the production of a single enantiomer gives way to a greater challenge turning attention to the racemic mixture separation by employing analytical methods. This review intends to offer a recent “state of the art” about separation of chiral compounds by nano and capillary liquid chromatography (LC). This overview is organized in two parts. The first one describes general considerations on nano-LC, including some theoretical features and a description of the experimental settings. In addition, enantiomers separation, chiral selectors and chiral stationary phase are also described in the first part or the manuscript. The second part includes the main applications in recent years, applications that confirm the great potential of nano-LC in the field of enantiomeric separation.

  • Microfluidics-integrated biosensing platforms as emergency tools for on-site field detection of foodborne pathogens
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-31
    Mihaela Puiu; Camelia Bala

    Early stage detection of pathogen in food matrices represents a central issue for public health and environment protection. New technologies using microfluidic platforms with disposable or reusable biosensors have emerged, aiming to match Affordable, Sensitive, Specific, User-friendly, Rapid and Robust, Equipment free, Deliverable to end users (ASSURED) criteria. Herein we have summarized the latest significant papers dedicated to microfluidics-integrated biosensors that are currently in use (or at least are amenable) for the on-site field detection of the most common foodborne pathogens. Microsystems containing surface plasmon resonance (SPR), surface acoustic wave (SAW), surface-enhanced Raman spectroscopy-based sensors, electrochemical- and paper devices (lateral flow test strips and electrodes), along with the most used capturing elements will be critically discussed, assessing the feasibility of their implementation in portable “sample-to-answer” devices.

  • State-of-the-Art of Methane Sensing Materials: A Review and Perspectives
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-30
    Tao Hong; Jeffrey T. Culp; Ki-Joong Kim; Jagannath Devkota; Chenhu Sun; Paul R. Ohodnicki

    Methane (CH4) is primarily used as a fuel for heating and electricity generation throughout the world. Despite its advantages such as high calorific value and low toxicity after combustion, CH4 is considered a greenhouse gas with a global warming potential greater than CO2 per molecule. CH4 is also explosive for concentrations above the lower explosive limit. Therefore, the development of high performing, low cost and ubiquitously deployable CH4 sensing materials could be beneficial from both environmental and safety perspectives. This article reviews sensing platforms and sensing mechanisms of current CH4 sensing materials, as well as summarizing their strengths and weaknesses. Metal Oxide, Carbon Materials, Conducting Polymers, Supramolecular Materials and Metal-Organic Frameworks based on Chemi-resistive, Optical and Electroacoustic platforms are discussed. Their operating conditions, sensing performances and limit of detection are carefully reviewed. Additionally, current challenges, including water vapor cross-sensitivity and selectivity, along with future perspectives are discussed.

  • Quantum Dots for Förster Resonance Energy Transfer (FRET)
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-29
    Marcelina Cardoso Dos Santos; W. Russ Algar; Igor L. Medintz; Niko Hildebrandt

    The analysis of biomolecular interactions using quantum dots (QDs) as both FRET donors and acceptors has become an established technique in the life sciences. This development has been driven by the unique properties of QDs, which include large surfaces for the attachment of biomolecules, high brightness and photostability, strong and spectrally broad absorption, and color-tunability via QD size, shape, and material. Applications include molecular rulers for structural analysis, small-molecule sensors, immunoassays, enzyme assays, nucleic acid assays, fluorescence imaging in-vitro and in-vivo, and molecular logic gates. Here, we will explain the theory of QD-based FRET, review some aspects of QD surface functionalization that are important for FRET, and highlight and discuss the advantages and disadvantages of QDs in FRET-biosensing using both spectroscopy and imaging techniques.

  • Advances and Applications of Stable Isotope Labeling-based Methods for Proteome Relative Quantitation
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-25
    Jianhui Liu; Yichu Shan; Yuan Zhou; Zhen Liang; Lihua Zhang; Yukui Zhang

    Proteins carry out numerous biological functions in living cells and form a dynamic system under various physiological and pathological conditions. Advances in proteome quantitation strategies have deepened our understanding of complex biological processes. Herein, we highlight stable isotope labeling-based quantitation methods and discuss the properties of each method, directed by the features of quantitation ions. Furthermore, the applications of such methods in quantifying a variety of protein properties, including post-translational modifications, subcellular localization, turnover rate, secretion and variation in multicellular environments, are also summarized.

  • 更新日期:2020-01-22
  • Liquid chromatography above 20,000 PSI
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-21
    Matthew J. Sorensen; Brady G. Anderson; Robert T. Kennedy

    Continued improvements in HPLC have led to faster and more efficient separations than previously possible. One important aspect of these improvements has been the increase in instrument operating pressure and the advent of ultrahigh pressure LC (UHPLC). Commercial instrumentation is now capable of up to ∼ 20 kpsi, allowing fast and efficient separations with 5 – 15 cm columns packed with sub-2 μm particles. Home-built instruments have demonstrated the benefits of even further increases in instrument pressure. The focus of this review is on recent advancements and applications in liquid chromatography above 20 kpsi. We outline the theory and advantages of higher pressure and discuss instrument hardware and design capable of withstanding 20 kpsi or greater. We also overview column packing procedures and stationary phase considerations for HPLC above 20 kpsi, and lastly highlight a few recent applications of ultrahigh pressure instruments for the analysis of complex mixtures.

  • Recent insights into functionalized electrospun nanofibrous films in chemo-/bio-sensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-18
    Tong Yang; Lei Zhan; Cheng Zhi Huang
  • 更新日期:2020-01-21
  • Frontiers In Electrochemical Enzyme Based Biosensors For Food And Drug Analysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-17
    Sevinc Kurbanoglu; Cem Erkmen; Bengi Uslu

    Nowadays, the development of various biosensors as a new generation of analytical instruments is one of the most promising research fields of analytical biology. Among all types of biosensors, enzyme based biosensors have an interesting property, the inherent inhibition phenomena given the enzyme-substrate complex formation. Over the past years, electrochemical enzyme based biosensors have emerged as simple, rapid and ultra-sensitive devices for determination or detection of different compounds in the drugs and food samples. In this review, general views to enzymes related with their history, classification, immobilization, and inhibition information is presented to researchers. Then, a detailed description is provided for enzyme based and electrochemical enzyme based biosensors. Finally, some selected electrochemical enzyme based biosensor studies developed for food and drug analysis are summarized and tabulated.

  • Chiral Capillary Electrophoresis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-10
    Samuel Bernardo-Bermejo; Elena Sánchez-López; María Castro-Puyana; María Luisa Marina

    The implications of chirality in different environments are already well known and reported extensively in the literature. Capillary Electrophoresis, a separation technique that only requires few nanoliters of sample, has demonstrated its potential for chiral analysis in the past years. The aim of this article is to provide an overview on the fundamentals and characteristics of Chiral Capillary Electrophoresis as well as the main advances and trends in this topic. Special attention is paid to the most recent technological and methodological developments achieved mainly in the most employed separation mode (Electrokinetic Cromatography). The most noteworthy and recent applications reported on the enantiomeric separation and determination of compounds in pharmaceutical, food, biomedical, environmental or forensic samples will also be critically overviewed. The characteristics of the developed methodologies will be detailed in Tables and future trends will also be discussed.

  • Recent advances in cancer early detection and diagnosis: role of nucleic acid based aptasensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-09
    Eman M. Hassan; Maria C. DeRosa

    Cancer is a complex disease of worldwide concern. Along with an improvement in outcomes, early and sensitive detection of cancer and cancer biomarkers can facilitate an understanding of cancer progression and aid in the development of effective treatment methods. Among the many detection methods available, aptasensors have shown great promise in recognition and identification of cancer cells and their biomarkers. In this review, we summarize the recent advances in optical and microfluidic aptasensors for early detection of cancer, as well as the recent developments in aptamer-based platforms for cancer exosome detection. The aptamer production process and their characterizations are initially introduced, and then the latest optical aptasensors for cancer cell recognition and detection are summarized. Microfluidic aptamer-based technologies for sensitive biomarker and cancer cell detection are then discussed. After that, the detection of cancer exosomes using recent fluorescent, colorimetric, and other approaches is elaborated. Finally, we provide a perspective on the future of aptasensors for cancer detection.

  • Gas chromatographic separation of enantiomers on novel chiral stationary phases
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-09
    Sheng-Ming Xie; Xue-Xian Chen; Jun-Hui Zhang; Li-Ming Yuan

    Chiral capillary gas chromatography has been widely applied to separate volatile and thermally stable enantiomers due to its advantages of simplicity, high efficiency, fast analysis, good sensitivity, and absence of liquid mobile phases. In the first part of this article, recent research progress (from 2010 to the present) concerning newly developed chiral stationary phases based on cyclofructan derivatives and chiral porous materials, including metal-organic frameworks, covalent organic frameworks, inorganic mesoporous silicas, and molecular cages for use in gas chromatography, and the synthetic strategies for obtaining chiral porous materials, was reviewed. Finally, we discussed in detail the separation performances and chiral recognition mechanisms of novel chiral recognition materials coated on capillary columns towards various types of enantiomers, namely (1) amino acid derivatives; (2) alcohols, amines, and amino alcohols; (3) organic acids; (4) aldehydes and ketones; (5) ethers and epoxides, and (6) esters and other enantiomers.

  • Recognition and Separation of enantiomers based on functionalized magnetic nanomaterials
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-09
    Xiaojuan Deng; Wenbin Li; Yi Wang; Guosheng Ding

    Enantioselective separation is significantly important for the study of pharmacology and biology, but it is still a big challenge because of the identical physical and chemical properties of enantiomers in an achiral environment. Magnetic nanomaterials have unique properties such as extremely large surface area per volume substance and strong magnetic response. It is very easy for them to be isolated from complicated matrixes, only by applying external magnets. The possibilities for surface functionalization make them exciting candidates for enantioseparation. Functionalized magnetic nanomaterials are unique tools for the simple and rapid enantioseparation compared with traditional chiral stationary phase. In this review, various magnetic nanomaterials modified with specific chiral selectors are reviewed in this manuscript. The prepared functional magnetic materials are typically used as chiral adsorbents for enantioseparation applications, formatted by methods of dispersive solid phase extraction, enantioselective crystallization, or column and microchip separation.

  • Plasmonics-attended NSET and PRET for analytical applications
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-08
    Peng Fei Gao; Yuan Fang Li; Cheng Zhi Huang
  • Chemical separation and characterization of complex samples with herbal medicine
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-07
    Qing Fu; Yanxiong Ke; Dasen Jiang; Yu Jin

    Herbal medicine has attracted great interest throughout the world. However, separation and characterization of components in herbs is still a challenge. Advanced techniques involved in this task, mainly including hydrophilic interaction liquid chromatography, supercritical fluid chromatography, two-dimensional chromatography and preparative chromatography, have showing an ongoing development in recent years. In this review, in addition to summarizing their progress and applications in herbal medicine in the last five years, their combination with mass spectrometry, activity screening or other techniques were introduced in detail.

  • Deep learning and artificial intelligence methods for Raman and surface-enhanced Raman scattering
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-07
    Félix Lussier; Vincent Thibault; Benjamin Charron; Gregory Q. Wallace; Jean-Francois Masson
  • Recent analytical advancements in microfluidics using chemiluminescence detection systems for food analysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-07
    Baqia Al Mughairy; Haider A.J. Al-Lawati

    Nowadays, the analysis of food is of high concern in terms of quality and safety. Microfluidic chemiluminescence have been devoted in vast food analyses proffering several merits and over performing different conventional methods in terms of sensitivity and selectivity. The ease of coupling with different separation techniques facilitated a comprehensive and adequate food analyses. This review explicitly focuses on the critical advancements in microfluidic chemiluminescence exploitation in food analyses based on the different pre-separation techniques utilized and the modes of coupling. Different dedications by microfluidic chemiluminescence systems including lab-on-a chip, μTAS, lab-on-a paper (μPADs) and lab -on- a cloth articles will be discussed and inspected.

  • 更新日期:2020-01-04
  • Recent advances in potentiometric biosensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-03
    Jiawang Ding; Wei Qin

    Potentiometry based on ion-selective electrodes (ISEs) has been injected with new vigor and gone through a renaissance with the improvements in the detection limits and selectivities of ISEs, the introduction of new materials, new sensing concepts (from conventional potentiometry to dynamic electrochemistry approaches), and deeper theoretical understanding and modelling of the potentiometric responses of ISEs. The new breakthroughs encourage innovations in ion sensing and biosensing applications. Moreover, with the introduction of new bioreceptors, such as enzymes, antibodies, aptamers, pepetides, versatile sensing protocols have been designed for a broad range of different target molecules by using ISEs as powerful transducers. This paper reviews the recent trends in potentiometric biosensors. Their applications in biosensing of metal ions, small molecules, DNA, proteins, bacteria and toxicities have been discussed. This review provides the outlook of the potentiometric biosensing based on the integration of potentiometric ISEs with new materials and emerging techniques.

  • Recent Advances in Differentiation of Isomers by Ion Mobility Mass Spectrometry
    Trends Anal. Chem. (IF 8.428) Pub Date : 2020-01-02
    Qian Wu; Jian-Ying Wang; Dong-Qi Han; Zhong-Ping Yao

    Differentiation of isomers is an important analytical task in many fields, e.g., chemistry, biology, pharmaceutical science, medicine, environmental science, food science. Ion mobility mass spectrometry (IM-MS) can resolve isobaric ions based on their shape and size and measure the collision cross section (CCS) values of ions to provide information on their intrinsic structures, and is thus a very promising tool for differentiation of isomers. In this review, the applications of IM-MS in differentiation of various isomers including glycans, lipids, amino acids, peptides, proteins, as well as metabolite isomers, chiral isomers, etc. are summarized. Prospects for further development of IM-MS for differentiation of isomers, e.g., improved resolving powers of IM-MS instruments, expanded CCS database and coupling of IM-MS with computational approaches, are also discussed.

  • Design of smart chemical 'tongue' sensor arrays for pattern-recognition-based biochemical sensing applications
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-30
    Zi-Han Chen; Qian-Xi Fan; Xin-Yue Han; Guoyue Shi; Min Zhang

    Conventional molecular recognition was tightly related to the development of analyte-specific sensor. However, all most every biochemical process are not decided on only one factor. When several factors were imported simultaneously, the established sensor could be seriously interfered and gave the wrong response. In this regard, chemical tongue-mimic sensor array was promoted to recognize a set of analytes, which concerned each component of mixtures. This review, focused on the development of chemical “tongue” sensor arrays from recent years, and gave enlightening examples about how to design integrated sensor system, how to choose the responsive signals and explain the mechanism of each used. As for applications, this review concluded the status of what chemical or biochemical species could be discriminated, what the advantages or drawbacks they had and the novel mechanism they used. In the last, this review also pointed out the limitations and future of related field.

  • Design Strategies of AuNPs-based Nucleic Acid Colorimetric Biosensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-30
    Tao Yang; Zewei Luo; Yonghui Tian; Cheng Qian; Yixiang Duan

    Nucleic acids are important biomarkers related to diseases of cancer, infectious, and genetic disorders. As a kind of indicator, gold nanoparticles (AuNPs) can generate colored signals from red to blue, which enables convenient signal readout. Based on the inherent optical properties, AuNPs are frequently used to fabricate colorimetric biosensors. Crosslinking colorimetry of polyvalent DNA-functionalized AuNPs and salt-induced colorimetry of unmodified AuNPs are two basic AuNPs-based colorimetric schemes. In order to improve such sensors sensitivity or add some specific functions, some amplification reactions or functionalized nanomaterials need to be introduced. Therefore, in this review, colorimetric biosensors with different functions and features are discussed, together with their advantages and limitations. By digging into the profound relationship between the various methods and strategies in this field, this review aims to provide a comprehensive reference outlining the general design strategies for constructing AuNPs-based nucleic acid colorimetric biosensors.

  • Selection and Applications of Synthetic Functional DNAs for Bacterial Detection
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-30
    Erin M. McConnell; Devon Morrison; Maria Alejandra Rey Rincon; Bruno J. Salena; Yingfu Li

    Early detection of an infectious bacterium is essential to preventing a potential outbreak or minimizing the impact of an active infection. Although prevailing methods are effective in many aspects, they suffer from being complex, expensive or insensitive. These drawbacks prevent their applications by the general public or under resource-limited settings. Therefore, developing rapid, accurate, inexpensive and sensitive methods for pathogenic bacterial detection remains a major research focus in the global research community. In recent years, functional nucleic acids, particularly DNA aptamers and DNAzymes, have been increasingly examined as the recognition element for bacterial biomarkers and whole bacterial cells. Significant efforts have also been made to utilize these affinity probes for the design of simple and cost-effective biosensors for bacterial detection. This review highlights the key achievements in this area, identifies important challenges, and provides thoughts on future directions.

  • Chemical sensor platforms based on fluorescence resonance energy transfer (FRET) and 2D materials
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-29
    Neema P. M; Ann Mary Tomy; Jobin Cyriac

    Enduring interests in two dimensional (2D) layered materials are attributed to their unique physicochemical properties. They are now extensively used in fluorescence-based chemical sensors as a quencher or as a fluorophore. In this review, we discuss recent progress in the field of chemical sensors that operate through fluorescence resonance energy transfer (FRET) in association with 2D materials. With a short introduction about the 2D materials, in general, we examine the luminescence properties of important 2D materials such as graphene, graphene oxide (GO), MoS2 and WS2. Various FRET-based sensors of these materials are presented after classifying them into FRET acceptors and FRET donors.

  • Saliva sampling: methods and devices. An overview
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-28
    Francesca G. Bellagambi; Tommaso Lomonaco; Pietro Salvo; Federico Vivaldi; Marie Hangouët; Silvia Ghimenti; Denise Biagini; Fabio Di Francesco; Roger Fuoco; Abdelhamid Errachid

    The continuous exchange of chemicals with blood and the non-invasive collection make saliva an interesting specimen for clinical applications, from the detection of biomarkers to the new –omic sciences in medicine. However, saliva sampling is challenging because the suitability of the collection method for the analyte of interest is either poorly investigated or, more often, not mentioned at all in most publications. This review reports a critical evaluation of the most common procedures for saliva sampling and a description of the off-the-shelf sampling devices. Their suitability for bioanalytical applications and salivary biomarkers detection, e.g. representativeness of the sample, sampling feasibility, analyte recovery, and sample amount, is discussed.

  • Recent advances in the bioanalytical and biomedical applications of DNA-templated silver nanoclusters
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-27
    Jiaqi Xu; Xuanmeng Zhu; Xi Zhou; Farjana Yeasmin Khusbu; Changbei Ma

    Silver nanoclusters stabilized with DNA scaffolds, with excellent physical and chemical properties, have emerged as a versatile tool in biomedical systems. Silver nanoclusters as a late-model nanomaterial with a small size less than 2 nm shows good stability and strong fluorescence. By using DNA with diverse sequence and structures, soluble DNA-templated silver nanoclusters are facilely prepared. They possess tunable fluorescence emission and are suitable with multifunctional designs. Extensive efforts have been put into the application potential of this biocompatible material for biosensing, bioimaging and therapy. Here we are committed to outline the recent advances of DNA-templated silver nanoclusters in biomedical application. The future prospect and challenges are then discussed considering rising progress. Though there is still a long way for illuminating the mechanism behind traits and conducting further clinical trials, we believe DNA-templated silver nanoclusters’ unprecedented unique properties will finally benefit medical progress.

  • Pure water as a mobile phase in liquid chromatography techniques
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Mikołaj Dembek; Szymon Bocian

    Liquid chromatography is a technique that is gaining increasing interest and application, for example in the pharmaceutical industry. At the same time as the increase in the number of analyses performed, the amount of organic waste produced while working with high-performance liquid chromatography (HPLC) apparatus is growing. Therefore, new methods and materials are being searched for to achieve the so-called "green" chromatography. In the following review we describe one of them, specifically the replacement of harmful organic solvents such as acetonitrile, methanol or isopropanol, with pure water used as the sole component of the mobile phase. In order to achieve a single component mobile phase, different methods or materials are used: the use of elevated temperature, the selection of new stationary phases, the utilization of changes in the properties of the stationary phase while using a highly polar eluent.

  • 更新日期:2019-12-23
  • FRET-based Nucleic Acid Probes: Basic Designs and Applications in Bioimaging
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Ke Quan; Cuiping Yi; Xiaohai Yang; Xiaoxiao He; Jin Huang; Kemin Wang

    Fluorescence imaging has emerged as one of the most robust techniques for noninvasive、real-time visualizing of biomolecules from in vitro to in vivo. Due to their high selectivity and sensitivity, ease of design and synthesis, suitability for structural modification, and minimum interference with living systems, FRET-based nucleic acid probes have been found instrumental for revealing the temporal and spatial resolution of biomolecules and biological process. In this review, we first give a brief introduction to the basic theory about FRET and fluorescent materials available for FRET, then particular attention is focused on typical designs of FRET-based nucleic acid probes and overview of the recent bioimaging applications of FRET-based nucleic acid probes from live cells to small animals. Furthermore, the challenges and prospects on future research directions of this promising field are also included.

  • Current overview and perspectives on carbon-based (bio)sensors for carbamate pesticides electroanalysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Thiago M.B.F. Oliveira; Francisco W.P. Ribeiro; Camila P. Sousa; Giancarlo R. Salazar-Banda; Pedro de Lima-Neto; Adriana N. Correia; Simone Morais

    Carbamate pesticides (CBMs) are esters and thioesters derived from carbamic acid, which are widely used to increase agricultural productivity and to protect human and animal health from insect-vector-mediated diseases. However, they can be highly toxic when not appropriately applied. Electrochemical carbon-based sensors and biosensors gather a plethora of advantages for reliable, low cost, rapid and even in loco quality control and surveillance of CBMs levels in all systems related to environmental and human health. Thus, this review presents a broad overview of the state-of-art of design and applications of these (bio)sensors for CBMs electroanalysis in environmental, food and biological matrices with emphasis in the role of the carbon (nano)materials. The latest advances and trends but also the main (bio)sensors limitations and research gaps are highlighted. Highly selective, robust and novel carbon-based (bio)sensors with multiplexing CBMs capability are envisaged to emerge shortly for in-field applicability.

  • Analysis of chiral drugs in environmental matrices: current knowledge and trends in environmental, biodegradation and forensic fields
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Ana Rita L. Ribeiro; Alexandra S. Maia; Cláudia Ribeiro; Maria Elizabeth Tiritan

    The challenge to develop enantioselective analytical methods to quantify residues of chiral drugs (CDs) in environmental matrices is an actual and imperative issue. It is well known that enantiomers may differ in their biological activities; nevertheless, most environmental analytical methods still ignore the stereochemistry and the discrimination of the enantiomers. The knowledge about their occurrence and ecological impact in biota is crucial for an accurate risk assessment. This critical review highlights the importance of analysing CDs in environmental matrices for various applications, emphasizing methodological trends. Chromatographic methodologies are the most used and include liquid (LC), gas (GC), supercritical fluid (SFC) and capillary electrophoresis. LC is still the most widely used though trends for greener approaches and the development of GC and SFC methods are also future directions. Some of the most recent applications, namely for environmental monitoring of surface and wastewater, biodegradation studies and forensic analysis, are presented and critically discussed.

  • Multifunctional aptasensors based on mesoporous silica nanoparticles as an efficient platform for bioanalytical applications: Recent Advances
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Houman Kholafazad Kordasht; Mahdiyeh Pazhuhi; Paria Pashazadeh-Panahi; Mohammad Hasanzadeh; Nasrin shadjou
  • Research progress on chemiluminescence immunoassay combined with novel technologies
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-23
    Qin Xiao; Chunxia Xu

    Chemiluminescence immunoassay (CLIA) has been greatly developed in the past several decades due to its good sensitivity and specificity. Nowadays, CLIA has been highly improved with novel nanomaterials and newly-developed technologies. The advancement of CLIA combined with relevant technologies are reviewed in the paper, including enhanced chemiluminescent, antibody preparation, aptamer selection, nanomaterials assisted CLIA, and CLIA coupling with newly-developed technologies. Finally, some critical challenges are discussed and the future development direction is prospected in CLIA. The review will be of great significance for CLIA basic research and practical applications in the fields of biomedical diagnostics, food and drug testing, environmental monitoring, and other fields.

  • Sample preparation and extraction methods for pesticides in aquatic environments: A review
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-18
    Maryam Nasiri; Hossein Ahmadzadeh; Amirhassan Amiri

    Pesticides are the most hazardous environmental pollutants due to their stability, mobility and long-term effects on living organisms. Their existence in sources of drinking water, essential to life, is a specific danger to living organisms. In water, these compounds may metamorphose to produce substances with even greater toxicity. Pesticide levels in diverse environments are generally below the limit of detection for many analytical instruments, so it is essential to isolate and then enrich pesticides from the complex matrix to decrease the interference effects in order to obtain a reliable signal. The main objective of this review is to evaluate the new sample preparation and extraction methods for the analysis of pesticides in various water samples.

  • Construction principles to modify responsive performance of fluorescent receptors: from background clearance to signal enhancement
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-18
    Peng Zhang; Qian Zhang; Lianxiao Zhang; Hua Zhong; Caifeng Ding

    There presents a rapid development for fluorescent sensory systems in recent years, appended with multiple functions, including organelle targeting, near-infrared emission, photothermal property and so forth. These in-depth studies extend the application area undoubtedly, but an excellent responsive behavior still needs a precise control. This Review focuses on the chemical modulation for responsive performance, which is discussed mainly in two parts, improvement of signal-noise ratio and signal amplification strategy. In addition to multifaceted literatures to illustrate the ratiometric strategy, comments on background-free platform are summarized elaborately, which propose a total clearance in background by in situ reactions. As for proposal to modify the responsive spectra, the control on chemical equilibrium, introduction of an additional “amplifier” and self-assembly-featured spectra manipulated by analytes are elucidated in detail, aiming to provide a straightforward pathway to constitute a set of well-behaved receptors integrating analytes-sensing with dye-distribution, avoiding the increasing demands for a complex probe.

  • Invited Review: Recent applications of FRET-based multiplexed techniques
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-17
    Anisa Kaur; Soma Dhakal

    Fluorescence resonance energy transfer (FRET) has become a powerful tool for visualizing molecular interactions, single-molecule conformational dynamics, binding, and other molecular signaling events. However, until recently, FRET spectroscopy/microscopy has been mainly used for monitoring single events. Recent pursuits on simultaneous detection and analysis of multiple analytes have led to the development of various FRET-based multiplexed techniques. The concomitant increase in the experimental complexity and data analysis due to the use of multiple donors/acceptor pairs and multiple excitation sources have proven to be the major challenges for rapid expansion of these techniques. In this review, we will focus on recent applications of FRET-based multiplexed techniques using coupled chromophore platforms composed of organic dyes, quantum dots (QDs), lanthanides, and fluorescent proteins, and discuss some of the current challenges that need to be addressed to further expand their applications in this new exciting area.

  • Recent advances in preparation and applications of monolithic chiral stationary phases
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-13
    Jialiang Guo; Qiqin Wang; Dongsheng Xu; Jacques Crommen; Zhengjin Jiang

    Chiral separation remains a very important topic in different fields of analytical chemistry such as pharmaceutical, agrochemical, environmental and food analysis. Over the last 20 years, various chiral monoliths, based on chiral selectors such as cyclodextrins and their derivatives, polysaccharide derivatives, proteins, antibiotics, chiral ligands, cinchona alkaloids and amino acid derivatives, as well as molecularly imprinted polymers, have been developed for the enantioseparation of a wide range of analytes. The present review summarizes the recent developments of monolithic chiral stationary phases (CSPs) with particular emphasis on their classification and preparation. The major challenges and future prospects of monolithic CSPs are also briefly discussed.

  • Advances in biosensing technologies for analysis of cancer-derived exosomes
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-13
    Huiying Xu; Bang-Ce Ye

    Exosomes play an important role in intercellular communication that contribute to the interaction of cancer cells with the tumor microenvironment. The original features endow tumor-derived exosomes with distinctive opportunities to serve as sensitive biomarkers for identifying early-stage tumors and monitoring of therapeutic response non-invasively. Conventional detection techniques face a number of drawbacks including sensitivity and reproducibility, therefore considerable efforts have been made to establish reliable biosensors for exosomes analysis. This review summarizes recent progress in exosomes quantification techniques, mainly focused on novel molecular recognition methods and signal transduction strategies. Furthermore, we offer a perspective of exosomes research towards the development innovative concepts on future applications.

  • AIE luminogens as fluorescent bioprobes
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-06
    Guangle Niu, Ruoyao Zhang, Xiujuan Shi, Hojeong Park, Sheng Xie, Ryan T.K. Kwok, Jacky W.Y. Lam, Ben Zhong Tang
  • Current advances in the detection of neurotransmitters by nanomaterials: An update
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-06
    Shiva Kumar Arumugasamy, Gayathri Chellasamy, Sivalingam Gopi, Saravanan Govindaraju, Kyusik Yun

    Neurotransmitters (NTs) are chemical messengers in the synaptic transmission process. NTs play important roles in human activities, and imbalanced NTs levels in the body lead to severe diseases such as Alzheimer’s and Parkinson’s diseases. The accurate diagnosis of NTs imbalances has been identified only after patient death through identifying plaques in the brain. Hence, the diagnosis and monitoring of NTs concentrations is important for avoiding serious illnesses. Many researchers have explored the use of robust diagnostic tools with various nanomaterials and techniques. Herein, we investigated the mechanism of reactions between NTs and nanomaterials, the role of nanomaterials in diagnostic tools and the different techniques used in the accurate detection of NTs. Furthermore, we discussed the simultaneous detection of NTs in a single sensing channel, detection in biological samples and future perspectives for nanomaterials in NTs detection.

  • Recent advances in electrochemiluminescence-based simultaneous detection of multiple targets
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Wanxue Lv, Haochen Ye, Zhiqin Yuan, Xuejiao Liu, Xu Chen, Wensheng Yang
  • Nano-impact Electrochemistry: Analysis of Single Bioentities
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Jian-Hua Zhang, Yi-Ge Zhou

    A central challenge of sensor technology is that the sensitivity of analytical detection is required to reach a single analyte entity level, whether it is a molecule, a cell or a nanoparticle. The emergence of nano-impact electrochemistry (NIE) allows in situ detection of single analyte entity one at a time with simplicity, fast response and high throughput. NIE method was originally designed to characterize physical and chemical properties of the corresponding single nanoparticles, and has been later extended into the field of bio-analysis, enabling better understanding of biological heterogeneity and providing new route for developing new diagnostic devices for quantifying biological analytes. A wide range of biological species including DNA, RNA, enzymes, bacteria, vesicles and cells has been already studied using NIE method so far. In this review, we first summarize the basic principles of NIE for bio-analyte detection and then elaborate NIE based bio-analysis categorized by analyte types. Finally, we give an outlook on the future prospects of this field.

  • A comprehensive approach to the analysis of antibiotic-metal complexes
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Sandra Ramotowska, Małgorzata Wysocka, Jakub Brzeski, Agnieszka Chylewska, Mariusz Makowski
  • Arsenic speciation analysis: A review with an emphasis on chromatographic separations
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-05
    Michael S. Reid, Karen S. Hoy, Jordan R.M. Schofield, Jagdeesh S. Uppal, Yanwen Lin, Xiufen Lu, Hanyong Peng, X. Chris Le

    More than 100 different arsenic species of diverse characteristics are present in the environment and biological systems. The identification and quantification of individual arsenic species are critical to understanding the distribution, environmental fate and behaviour, metabolism, and toxicity of arsenic species. This review summarizes sample preparation, separation, detection, and method validation for arsenic speciation analysis. An emphasis is placed on chromatographic separation techniques, relating the physicochemical properties of arsenic species to their efficient separation. Anion exchange, cation exchange, reversed-phase, ion pair, and size exclusion chromatography are useful to separate various arsenic species. Recent research has explored hydrophilic interaction liquid chromatography (HILIC), multiple separation mechanisms, and testing of fluorophenyl and graphene oxide stationary phases for the separation of arsenic species. Sample preparation, extraction of arsenic species, recovery of arsenic species from separation columns, and method validation are discussed in light of their importance to the integrity and accuracy of speciation analysis.

  • Ultraspecific analyte detection by direct kinetic fingerprinting of single molecules
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-04
    Tanmay Chatterjee, Zi Li, Kunal Khanna, Karen Montoya, Muneesh Tewari, Nils G. Walter, Alexander Johnson-Buck

    The detection and quantification of biomarkers have numerous applications in biological research and medicine. The most widely used methods to detect nucleic acids require amplification via the polymerase chain reaction (PCR). However, errors arising from the imperfect copying fidelity of DNA polymerases, limited specificity of primers, and heat-induced damage reduce the specificity of PCR-based methods, particularly for single-nucleotide variants. Furthermore, not all analytes can be amplified efficiently. While amplification-free methods avoid these pitfalls, the specificity of most such methods is strictly constrained by probe binding thermodynamics, which for example hampers detection of rare somatic mutations. In contrast, single-molecule recognition through equilibrium Poisson sampling (SiMREPS) provides ultraspecific detection with single-molecule and single-nucleotide sensitivity by monitoring the repetitive interactions of a fluorescent probe with surface-immobilized targets. In this review, we discuss SiMREPS in comparison with other analytical approaches, and describe its utility in quantifying a range of nucleic acids and other analytes.

  • Enantioseparation using helical polyacetylene derivatives
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-04
    Chunhong Zhang, Lijia Liu, Yoshio Okamoto

    In the past few decades, the enantioseparations techniques have been quickly developed in order to meet the needs of single enantiomers in many fields. Polyacetylene derivatives have attracted considerable attention due to their rigid conjugated backbone with high stereoregularity that can be readily induced into a regular helical conformation by the chiral bias covalently or non-covalently bonding to the backbone. Macromolecular helicity plays an important role in enhancing the enantioseparation ability, and the controllable and stereoregular helical structure of the polyacetylenes affords them a wide variety of potential applications, which are promising candidates for enantioseparation. In this review, the discovery and development of helical polyacetylene derivatives and the enantioseparations using helical polyacetylene derivatives as chiral stationary phases (CSPs) for high-performance liquid chromatography (HPLC), optical resolution membranes, enantioselective adsorbents and chiral additives for enantioselective crystallization will be described.

  • Recent Advances in Background-Free Raman Scattering for Bioanalysis
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-04
    Xuehui Liu, Xiaoman Liu, Pengfei Rong, Dingbin Liu

    With the characteristics of intrinsically free from photobleaching, blinking, and self-quenching, Raman microscopy has become a powerful platform in detecting molecular vibrations for diverse applications. In particular, Raman spectroscopy has proven very useful for bioanalysis but suffers from several challenges such as poor sensitivity, spectral overlapping, and background interference. In this review, we would like to introduce two kinds of high-fidelity imaging strategies, including background-free surface-enhanced Raman scattering (SERS) and stimulated Raman scattering (SRS) that are capable of significantly enhancing detection sensitivity and spectral resolution while minimizing background interference simultaneously. Recent advances in background-free SERS and SRS and their applications in biological detection and cellular imaging are summarized and discussed.

  • Chiral analysis in food science
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-03
    Gerardo Alvarez-Rivera, Mónica Bueno, Diego Ballesteros-Vivas, Alejandro Cifuentes

    Determination of enantiomeric ratios in food and beverages is a research area of great interest because enantiomers can exhibit different biological activities. Chiral analysis can provide valuable information about food safety, bioactivity, quality and traceability. In this work, the potential of separation techniques such as liquid chromatography, gas chromatography, supercritical fluid chromatography and capillary electrophoresis for chiral analysis of food is reviewed. The analysis of enantiomers based on other chiral methodologies such as countercurrent chromatography, sensors, biosensors and direct mass spectrometry (MS) analysis is also included. The present review article provides an updated perspective on the importance of chiral analysis in food matrices and beverages covering the period from February 2017 to July 2019, following the previous work published in this journal on the same topic (G. D'Orazio et al., TrAC-Trends in Analytical Chemistry 96 (2017) 151-171).

  • Non-linear mass transport in confined nanofluidic devices for label-free bioanalysis/sensors
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-03
    Fei-Fei Liu, Xiao-Ping Zhao, Bin Kang, Xing-Hua Xia, Chen Wang

    In the past decade, a significant growth of research in nanofluidics is achieved. The electric double layer (EDL) overlapped in the nanochannel/nanopores results in non-linear mass transport. To understand the principle of non-linear mass transport in nanofluidics can help us to control and manipulate mass transfer and find applications in varied fields such as energy conversion and storage, gas separation, and bioanalysis/sensor. In this review, we first introduce the fabrication of nanofluidic devices, then demonstrate the origin mechanism of non-linear mass transport, among which the size and charge effect of nanofluidics are explained. Following that the influential factors regarding the non-linear mass transport behaviors are discussed in detail. After full understanding of the fundamental of non-linear mass transport in nanofluidics, the recent progress in bioanalytical applications based on nanofluidic non-linear transport are highlighted. Finally, the perspectives for future directions and development are discussed for this special research field.

  • Chiral Mass Spectrometry: An Overview
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-03
    Dong-Qi Han, Zhong-Ping Yao

    Chiral analysis by mass spectrometry has attracted much attention due to its advantages in speed, sensitivity and specificity. In this review, recent advances in chiral analysis by mass spectrometry are summarized, with the methods based on tandem mass spectrometry (e.g., kinetic method, chiral recognition ratio method and photodissociation mass spectrometry method) and ion mobility mass spectrometry highlighted. Different methods are compared, and the limitations of the current studies and the prospects of chiral mass spectrometry are discussed.

  • Resonance energy transfer to gold nanoparticles: NSET defeats FRET
    Trends Anal. Chem. (IF 8.428) Pub Date : 2019-12-02
    Chi Chen, Niko Hildebrandt

    Gold nanoparticles (AuNPs) have been widely applied as highly efficient luminescence quenchers in combination with a large variety of donor fluorophores. While the application of such systems in optical biosensing has resulted in significant advances of biological, biochemical, and biophysical research, the underlying energy transfer mechanism has been much less investigated. In this review, we discuss the differences between Förster resonance energy transfer (FRET) and nanosurface energy transfer (NSET), give an overview of the literature concerning the assignment of FRET or NSET to AuNP-based luminescence quenching, show why the development of improved NSET models and their application to experimental data provided strong arguments in favor of the NSET formalism, and discuss applications of NSET in biosensing, molecular rulers, and molecular tension probes. Taking into account the existing literature, the NSET formalism, including size and wavelength-dependent issues, is superior to FRET theory for a general description of energy transfer to AuNPs.

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