The giant freshwater prawn (GFP) Macrobrachium rosenbergii is a commercially important cultured species that originates from tropical and subtropical regions and has poor cold tolerance. This study investigated the species’ cold tolerance and regulation patterns of the related response genes, using GFP juveniles subjected to extreme low-temperature treatment. The water temperature was decreased from

During a parasitological survey of goldfish Carassius auratus (Linnaeus, 1758) in China, two myxosporeans were collected in the present study including a Thelohanellus species and a Myxobolus species. To identified the two myxosporeans, a morphological, histological and molecular study was performed. The Thelohanellus species was characterized by round, white plasmodia in fins of goldfish. Compared

We aimed to develop a multiplex PCR method for the simultaneous detection of the three most consumed commercial loaches in East Asia: Misgurnus mizolepis, Misgurnus anguillicaudatus, and Paramisgurnus dabryanus. Phylogenetic analysis of genetic divergence and a phylogenetic tree based on the cytochrome oxidase I gene enabled successful genetic identification of the 3 species of loach. Multiplex species-specific

Interleukin-6 (IL-6) is a multifunctional cytokine involved in innate and acquired immunity. In mammals, IL-6 can induce the secretion of acute-phase proteins (APPs) and antimicrobial peptides, activate macrophages and neutrophils, and regulate the expression of inflammatory cytokines in innate immunity, resulting in enhanced resistance to invading pathogens. In teleost fish, the functions of IL-6

To examine the growth and immune response of steelhead trout (Oncorhynchus mykiss) under conditions of differing light color, we investigated the effects of different red and blue light color combinations on plasma non-specific immunity, hepatic antioxidant capacity, and GH/IGF-1 axis gene expression in juvenile steelhead trout (initial weight: 34.67 ± 2.69 g). The 16-week trial was set up with six

To explore the effect of ferulic acid (FA) on growth and nonspecific immunity of Macrobrachium nipponense, six experimental diets were supplemented with 0 (FA0), 20 mg/kg (FA20), 40 mg/Kg (FA40), 80 mg/kg (FA80) 160 mg/kg (FA160), 320 mg/kg (FA320) ferulic acid, respectively. M. nipponense (initial weight: 0.140 ± 0.015 g) was randomly assigned into 6 groups with 6 replicates for 10 weeks. The results

As antibiotics-treated animal models provide good insights into the potential causes of the microbiota-dependent changes, we explored the effects of microbiota perturbation driven by a mixture of antibiotics on lipid metabolism and lipid deposition in the liver of hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) fed a bile acid diet in this study. Ninety groupers were randomly fed a bile

This study aimed to evaluate the effects of glutamine supplementation to soybean meal (SBM) diet in hybrid sturgeon (25.00 ± 1.00 g). Three diets, including fishmeal (FM) diet, SBM diet, and SBMG diet (glutamine addition to SBM diet), were formulated to feed sturgeons for 8 weeks. Growth parameters and muscular crude lipid content were reduced by SBM substitution, and were further increased in SBMG-diet

Tilapia lake virus (TiLV) is the main tilapia-infecting virus worldwide, causing serious economic losses to the tilapia aquaculture. However, there is no vaccine for this viral disease. Here, TiLV ORF10 (TiLV-ORF10) encoding a protein with abundant epitopes was cloned into the eukaryotic expression vector pcDNA3.1 as a vaccine candidate, and then used to evaluate the protective effects against TiLV

In order to accelerate the application of ABPs in shrimp and crab feed, we evaluated the effect of ABPs on the growth, non-specific immunity and disease resistance of crayfish. Crayfish (Procambarus clarkii) were randomly divided into six groups, the fed diets supplemented with different levels of ABPs: 0, 0.05, 0.15, 0.3, 0.45 and 0.6 g/kg. After 28 days of feeding, ABPs were found to significantly

IL-10 cytokine family belongs to type Ⅱhelix cytokine, which has many common characteristics and different biological functions. In this paper, the IL-10 and IL-20L genes in the IL-10 family of swamp eel (Monopterus albus) were characterized for the first time, and their expression patterns after stimulation by Aeromonas veronii were studied. Through phylogenetic tree, amino acid sequence alignment

Astakine is a cytokine of invertebrates that promotes the proliferation and differentiation of hematopoietic stem cells, which regulates hematopoietic and immune responses. In this study, we have identified an astakine homolog (MrAst) from the freshwater prawn Macrobrachium rosenbergii. The MrAst cDNA consists of 408 bp with a 390 bp open reading frame (ORF) encoding 129 amino acids. MrAst contains

An 8-week feeding trial was carried out to explore the effects of fish meal replacement with low-gossypol cottonseed meal, a potential alternative protein source, in juvenile turbot (Scophthalmus maximus L.). Five isonitrogenous and isoenergetic diets, with different substitutional levels (0%, 15%, 25%, 35% and 45%, respectively), were formulated. The growth performance, feed utilization and haematological

This study was to compare study on the effects of supplemental L-carnitine on fatty acids composition, energy status and related genes expression in Cyprinus carpio var. Jian and Rhynchocypris lagowskii. Different concentrations of L-carnitine were added to the basal diets of two species of fish, in which Cyprinus carpio were supplemented with 0, 50 (LC), 200 (AC) and 800 (HC) mg/kg, and Rhynchocypris

Probiotics are an eco-friendly alternative to antibiotics and play an important role in ensuring the sustainability of aquaculture. The aim of the present study was to investigate the effects of Saccharomyces cerevisiae on the growth, intestinal and liver health, intestinal microbiota, and disease resistance of channel catfish (Ictalurus punctatus). Channel catfish were randomly allotted into two treatments

The purpose of this study was to investigate the effect of an activation solution (AS) (hatchery water (pH 7.9 and 4 mOsm/kg), Perchec solution (5 mM KCl, 45 mM NaCl, 30 mM Tris-HCl; pH 8.0 and 130 mOsm/kg) and Cheng solution (40 mM NaCl, 20 mM Tris; pH 8.0 and 100 mOsm/kg) on aging sperm of common carp (Cyprinus carpio) after storage in a carp artificial seminal plasma (carp ASP: 2 mM CaCl2, 1 mM

Hypoxia occurrence is potentiated by eutrophication caused by human activities and it may be the most dramatically increasing environmental factor as a result of the climate change-led increasing temperatures. The crucian carp Carassius auratus is an important aquaculture economical freshwater species and is also a potential model for the study of physiological adaptation. However, the transcriptomics

Genetic improvement of feed efficiency is key to improve the economic and environmental sustainability of fish farming. However, it requires individual phenotypes of feed efficiency, which are difficult if not impossible to obtain when fish are reared in tanks or cages. Here, we applied and validated on gilthead sea bream a method to evaluate individual feed efficiency based on individual rearing of

Farmed fish and shrimp are continuously challenged by multiple stressors during their life stages, such as hypoxia, pH fluctuations, different salinities, high nitrite, un-ionized ammonia, injury during handling, inadequate nutrition, or food shortage, which can eventually adversely impact their health, welfare, and growth rates. Besides, these stressors can weaken production and decrease their resistance

Grass carp (Ctenopharyngodon idellus, C.idellus) is one of the most important globally cultured freshwater fish species, and its growth trait can be improved by dietary supplementation of oligopeptides which is transported through the intestinal oligopeptide transporter PepT1. However, many aspects of the molecular regulation mechanism of C.idellus PepT1 still remains elusive. In this study, the regulation

Inland marine shrimp production is increasing in popularity as recirculating aquaculture systems (RAS) can provide a fresh, high-value product year-round to nearby consumer markets. However, artificial sea salt mixtures are required for inland production, which can be a substantial portion of the production costs. The purpose of this experiment was to compare marine shrimp (L. vannamei) production

The Biotronic® Top3 (OCAP: organic acids, cinnamaldehyde, and permeabilising complex) is used for improving microbial community in monogastric animals. This research was aimed to test the five dosages of OCAP (0%: control, 0.05%: OCAP0.05, 0.10%: OCAP0.1, 0.15%: OCAP0.15%, and 0.20%: OCAP0.2) in barramundi (Lates calcarifer) (8.3 ± 0.3 g) for eight weeks. The results showed a linear relation between

Traditional agricultural systems demonstrated farmers' wisdom in farming, while extending many new agricultural ecosystems and breeding a lot of new and interesting organisms. This study introduced the discovery of an indigenous golden-back crucian carp (GBCrC; Carassius gibelio) from the Dong's Rice-Fish-Duck System, one of the Globally Important Agricultural Heritage Systems in the world, by exploring

The experimental trial was conducted to assess the effects of dietary pyridoxine on growth performance, physiological metabolics, intestinal enzymatic activities and antioxidant properties of Pelteobagrus fulvidraco. Six isonitrogenous and isolipidic diets (420 g/kg protein and 70 g/kg lipid) were formulated to contain 3.69 (control), 5.45, 7.31,11.17, 19.43 and 33.94 mg/kg pyridoxine. Four groups

The combined use of satellite-derived environmental data and a dynamic energy budget (DEB) model to determine Pacific oyster growth potential was adapted for the South African marine environment. Study areas consisted of the West Coast (high-chlorophyll, low temperature) and the South Coast (variable chlorophyll, higher temperature) ecoregions. Chlorophyll-a and sea surface temperature products from

This study aimed to investigate the effects of the glycerol monolaurate (GML) and tributyrin (TB) and their interaction on the growth performance, body composition, nonspecific immunity, gene expression and intestinal microbiota of Litopenaeus vannamei (initial weight 0.24 ± 0.00 g). The feeding trial comprised a completely randomized 3 × 2 factorial design and six formulated diets with two different

Fish meal (FM) usage in aquafeed is fraught with cost and environmental challenges. Alternatives to FM must be readily available and with adequate protein. This 8-weeks study explored the utilization of tuna by-product meal (TBM) without fermentation as a substitute for FM in diet on growth, feed utilization, chemical composition, plasma chemistry, and non-immune responses of olive flounder (Paralichthys

Knowledge of population structure and genetic diversity within and between wild and farmed populations of gilthead sea bream (Sparus aurata) and European seabass (Dicentrarchus labrax) is important to achieve sustainable aquaculture production of these species and to assess the risk of genetic impacts of fish escaped from farms. Previous population genetic studies on these species have been based on

The present study conducted to evaluate the impact of dietary C: N: P ratios on Nile tilapia, growth induces in biofloc system and formation of water biotic communities. Five isonitrogenous, 30% crude protein and isocaloric 23.35 MJ/kg gross energy were formulated. Diets were equal but with variation of mono ammonium phosphate, NH₄H₂PO₄ levels to obtain different Carbon/Nitrogen/ Phosphorus (C/N/P)

The estrogen-related receptors (ERRs), a group of nuclear receptors, are the first orphan receptor to be identified. In this study, six errs (errα, errβ, errγ, errγA, errγB, errδ) were first cloned from the gonads of mandarin fish (Siniperca chuatsi). Gene cloning results showed the open reading frames (ORF) of both errα, errβ, errγ, errγA, errγB, errδ were 1302 bp, 1434 bp, 1323 bp, 1566 bp and 1308 bp

Environmental factors affect the biological functions of aquaculture species both in the wild and in captivity. Therefore, optimization of the husbandry conditions under captivity can improve the production performance of an aquaculture species. In this study, the effect of water velocity on the glucose concentration, energy level of different body parts (i.e., hepatopancreas, gonad, muscle, and feces)

In recent years, the dramatically high lipid level has been used in fish feeds, resulting in low survival rates of fish and huge economic losses. Based on these issues, a six-week feeding experiment was conducted to investigate whether diet supplements with L-carnitine can be used to relieve detrimental impacts on growth performance, hepatic lipid accumulation, antioxidant, anti-inflammatory and non-specific

An imbalance in the production and detoxification of reactive oxygen species and other oxidants can disrupt all types of cellular compounds, and lead to a state of oxidative stress. Preventing this state is essential to improve growth and health in animal production. Therefore, the aim of this work was to assess if the dietary supplementation of curcumin could improve the antioxidant status and intestine

Exposure to nutritional stress during early ontogeny can generate latent effects that are carried over to later developmental stages and affect their growth performance. However, the compensatory role played by food quality at such later stages is not well known in invertebrates with bi-phasic life cycles. Here, larvae of the caridean shrimp Palaemon varians were either allowed to feed immediately

The study aimed to investigate the effects of four fatty acids with the same carbon-chain length and different saturations, stearic acid (SA), oleic acid (OA), linoleic acid (LA), and linolenic acid (LNA), on the growth performance and muscle quality of Megalobrama amblycephala through in vivo and in vitro experiments. A total of 320 fish with similar initial weight (34.97 ± 0.14 g) were randomly fed

Aquaculture is now a viable alternative to help depleted wild fish stocks and encourage the production of alternative animal protein sources. However, studies have shown that farmed fish has a less positive image in consumers than wild fish. The present study investigates the aspects that most influence the consumption choices of some of the most farmed species in the Mediterranean, sea bass and sea

Sex plasticity is easily influenced by external conditions in teleosts. In the present study, the morphogenesis of gonad development was described, and the sex ratio and gonadal development were also analysed after induction by exogenous E2. The primordial gonad was first visible at 9 dph, and PGCs entered mitosis at 90 dph. The time of transition from single oogonia to clusters of oogonia was from

A feeding trial was conducted to assess the effects of nano-selenium (n.Se) supplementation in a plant protein (PP)-based diet on female Acanthopagrus arabicus broodfish and their offspring. Two basal diets were prepared: a fish meal (FM)-based diet (FM-control) as a positive control and a PP-based diet. The PP-based diet was supplemented with n.Se at five levels including 0 (PP-control, as a negative

An 8-week feeding study was conducted to examine how dietary pantothenic acid (PA) affects growth, antioxidant ability and innate immunity in juvenile black carp (Mylopharyngodon piceus). Juvenile fish (7.35 ± 0.06 g) were fed diets with PA at 0, 5, 10, 20, 40 and 80 mg/kg, respectively. The highest weight gain (WG), specific growth rate (SGR) and protein efficiency ratio (PER) were obtained with 10 mg/kg

The aim of this study was to determine the effects of Pro-A (a mixture of Bacillus subtilis, B. licheniformis, B. pumilus) on the growth performance, feed utilization, immune responses, and intestinal microbiota of whiteleg shrimp (Litopenaeus vannamei). The experiment was conducted with four iso-nitrogenous (45.4% crude protein) and iso-caloric (19.9 KJ g−1) diets supplemented with different levels

In this study, the dietary effect of garlic powder (GP) as an herbal supplement on the growth performance, digestive enzymes, innate immunity, and antioxidant capacity of narrow-clawed crayfish, Postantacus leptodactylus, was investigated. In a 90-day feeding trial, the crayfish (N = 360; 25.90 ± 0.41 g initial weight) were fed with a basal diet (control; GP-free) and supplemented diets with three

Pseudomonas aeruginosa is a common gram-negative pathogen belonging to the Aeromonas genus. In the present study, dominant bacteria were isolated from diseased crucian carp (Carassius auratus gibelio). The isolate was identified as P. aeruginosa based on biochemical identification and 16S rDNA sequencing. In the histological observation of diseased C. auratus, a large number of vacuoles and inflammatory

Determination of nutrient requirements for a cultured fish species is necessary to develop a nutritionally-balanced and cost-effective diet because feed contributes greatly to aquaculture operation costs. Lipids are crucial nutrients that provide essential fatty acids, phospholipids, and energy. Thus, the current study was conducted to determine an optimum dietary lipid level for juvenile hybrid pufferfish

Triploidy is induced in Atlantic salmon (Salmo salar) to produce sterile fish for genetic containment and to hinder early sexual maturation in farmed fish, but it can have unwanted negative effects on growth, health, and welfare. However, the growth and welfare of triploid fish may be improved by adjusting the rearing environment, feeding conditions and diets. This study evaluated physiological changes

A four-week feeding trial, followed by a challenge test, was conducted to evaluate the effects of Lactococcus lactis and astaxanthin on the growth performance, digestive ability, antioxidant capacity, immunity, and disease resistance of Litopenaeus vannamei against Vibrio alginolyticus. Specifically, shrimp were randomly allocated into 12 tanks (100 shrimp per tank), and divided into 4 groups with

Oxidized fish oil, as a harmful ingredient in feed, has a negative impact on aquatic animals. But the effect of oxidized fish oil on hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ Epinephelus lanceolatus), especially the intestinal flora, is not yet understood. In the current study, a 65-day raising experiment with diets containing 0 g/kg, 30 g/kg, 60 g/kg, and 90 g/kg oxidized fish oil was performed

Sustainable aquaculture requires the efficient use of natural pond resources as well as supplements to meet the dietary requirements of fish species. However, aquaculture diets are often produced without considering the lysine content of the natural production in the pond. As such, two plant-based diets, a control diet (L-Con) supplemented with 5.00 g lysine kg−1 diet and a lysine deficient diet (L-Def)

Understanding the effects of dietary nutrients on physiological and biochemical parameters, especially the metabolism of nutrients during crayfish farming, can be useful for improving crayfish health and optimizing production in aquaculture. Therefore, we conducted an 8-week feeding study to investigate the effect of dietary carbohydrate-to-lipid (CHO: LIP) ratios on growth performance, hemolymph biochemical

A novel technologically advanced fermented wheat gluten and yeast sourced protein (FWPC) co-product utilising Saccharomyces cerevivisiae yeast was evaluated for juvenile Nile tilapia Oreochromis niloticus. The protein component of a semi-purified basal diet (control treatment) was incrementally replaced by Fermented Wheat Protein Concentrate (FWPC) at 10%, 20%, and 30% of total dietary protein to create

Hybrid lemon fin barb is a novel carp species produced in Malaysia crossing lemon fin barb (Hypsibarbus wetmorei, ♂) and silver barb (Barbonymus gonionotus, ♀). As the delicious wild H. wetmorei is being overexploited, this new hybrid species is produced, filling the production gap in Malaysian aquaculture. The hybrid larvae in our previous study showed high tolerance to bacterial diseases and revealed

While highly successful in terms of profitable seafood production, salmon (Salmo salar) aquaculture may also be a source of potential negative environmental externalities. In an attempt to address these challenges through supporting the development of new technology, the Norwegian government has introduced a new class of aquaculture licenses labeled as development licenses. As a result, new technological

This study was performed to compare the effects of high carbohydrate and high lipid diets reception on the growth, feed utilization and glucose homeostasis of genetically improved farmed tilapia Oreochromis niloticus. Three isonitrogenous (ca 30.2% protein) diets were formulated with different levels of starch and lipid: the control (CON, 24.2% starch and 6.57% lipid), high carbohydrate diet (HCD,

This study was conducted to investigate the impacts of potassium diformate (KDF) on growth performance, fillet quality, plasma indices, immune response and intestinal morphology of juvenile Trachinotus ovatus. Six diets with 0 (KDF1), 3.00 (KDF2), 6.00 (KDF3), 9.00 (KDF4), 12.00 (KDF5) and 15.00 (KDF6) g/kg KDF were formulated to feed T. ovatus (average fish weight: 5.87 ± 0.03 g) for 8 weeks. The

This study describes the shell color polymorphism and its correspondent metabolic activities in hatchery-reared Pinctada maxima juveniles. Analysis of color distances assessed the similarities among the different phenotypes (Ph); from the most common Ph-1 (gray), Ph-2 (bronze), Ph-3 (white), Ph-4 (brown), Ph-5 (green), Ph-6 (purple), and Ph-7 (yellow). Yellow-colored shells (Ph-7) were found less unrelated

The present study was performed to examine the effects of aquatic fern (Azolla caroliniana) (AQF) on growth performance, skin mucus and serum immunities, and disease resistance of Nile tilapia reared in a biofloc system. Three hundred Nile tilapia fingerlings (average 10.48 ± 0.56 g fish-1) were distributed into 15 glass tanks (150 liters tank−1) at a concentration of 20 fish tank−1. A completely

An 8-week growth trial was conducted to assess the effects of dietary vitamin C (VC) on the growth performance, digestive enzyme activity, antioxidant ability, immunity, and resistance to Vibrio harveyi infection of coral trout (Plectropomus leopardus, initial weight 17.42 ± 0.06 g). Four kinds of iso-nitrogenous and iso-lipidic experimental feeds were formulated with covering VC levels of 0 (C0),

The aim of this study was to investigate the effects of replacing different proportions of fishmeal with Clanis bilineata larvae (CBL) on the growth performance, body composition and non-specific immune index of white shrimps (Penaeus vannamei). CBL was used to replace 0 (CBL0), 25% (CBL25), 50% (CBL50) and 100% (CBL100) of the fishmeal in the basic diet to formulate four kinds of test feeds with basically