Stable isotope signatures of elements related to life such as carbon and nitrogen can be powerful biomarkers that provide key information on the biological origin of organic remains and their paleoenvironments. Marked advances have been achieved in the last decade in our understanding of the coupled evolution of biological carbon and nitrogen cycling and the chemical evolution of the early Earth thanks

The plant cell wall provides each cell with structural support and mechanical strength, and thus, it plays an important role in supporting the plant body against the gravitational force. We investigated the effects of microgravity on the composition of cell wall polysaccharides and on the expression levels of genes involved in cell wall metabolism using rice shoots cultivated under artificial 1 g and

Serpentinization is a weathering process in which ultramafic rocks react with water, generating a range of products, including serpentine and other minerals, in addition to H2 and low-molecular-weight hydrocarbons that are capable of sustaining microbial life. Lipid biomarker analyses of serpentinite-hosted ecosystems hold promise as tools for investigating microbial activity in ancient Earth environments

Critical to the success of establishing a sustainable human presence on Mars is the ability to economically grow crop plants. Several environmental factors make it difficult to fully rely on local resources for agriculture. These include nutrient sparse regolith, low and fluctuating temperatures, a high amount of ultraviolet radiation, and water trapped locally in the form of ice or metal oxides. While

Polygonal features in a ∼250 million-year-old Permian evaporitic deposit were investigated for their geological and organic content to test the hypothesis that they could preserve the signature of ancient habitable conditions and biological activity. Investigations on evaporitic rock were carried out as part of the MIne Analog Research (MINAR) project at Boulby Mine, the United Kingdom. The edges of

A previously proposed synthesis of pyrimidine ribonucleotides makes use of ultraviolet (UV) light to convert β-d-ribocytidine-2′,3′-cyclic phosphate to β-d-ribouridine-2′,3′-cyclic phosphate, while simultaneously selectively degrading synthetic byproducts. Past studies of the photochemical reactions of pyrimidines have employed mercury arc lamps, characterized by narrowband emission centered at 254 nm

This work investigated microorganism survival under temperature and ultraviolet (UV) radiation conditions found at the surface of ice-covered ocean worlds. These studies were motivated by a desire to understand the ability of resilient forms of life to survive under such conditions as a proxy for potential endogenic life and to inform planetary protection protocols for future missions.

One of Saturn's largest moons, Enceladus, possesses a vast extraterrestrial ocean (i.e., exo-ocean) that is increasingly becoming the hotspot of future research initiatives dedicated to the exploration of putative life. Here, a new bio-exploration concept design for Enceladus' exo-ocean is proposed, focusing on the potential presence of organisms across a wide range of sizes (i.e., from uni- to multicellular

The Hayabusa2 mission successfully collected samples from the asteroid Ryugu last year and will return these to Earth in December 2020. It is anticipated that the samples will enable the analysis of terrestrially uncontaminated organic matter and minerals. Such analyses are in turn expected to elucidate the evolution of organic matter through Solar System history, including the origination and processing

Chocolate Pots hot spring (CP) is an Fe-rich, circumneutral-pH geothermal spring in Yellowstone National Park. Relic hydrothermal systems have been identified on Mars, and modern hydrothermal environments such as CP are useful for gaining insight into potential pathways for generation of biosignatures of ancient microbial life on Earth and Mars. Fe isotope fractionation is recognized as a signature

The InSight (Interior Exploration using Seismic Investigations, Geodesy and Heat Transport) Mars flight system is made up of a cruise stage, an aeroshell, and a Phoenix heritage-based lander that successfully landed in the Elysium Planitia on November 26, 2018. InSight has primary science objectives that are aimed at understanding more about the interior of Mars and the formation and evolution of terrestrial

It is now routinely possible to sequence and recover microbial genomes from environmental samples. To the degree it is feasible to assign transcriptional and translational functions to these genomes, it should be possible, in principle, to largely understand the complete molecular inputs and outputs of a microbial community. However, gene-based tools alone are presently insufficient to describe the

The Special Collection of papers in this issue of Astrobiology provides an overview of the characteristics and potential for future exploration of the Ojos del Salado volcano, located in the Andes Mountains in front of the Atacama Desert in northern Chile. The main benefits of this site compared with others are the combination of strong UV radiation, the presence of permafrost, and geothermal activity

Hourly ground temperature measurements from the highest shallow ground temperature monitoring system on Earth and sedimentological data were used to construct a thermal model at the Ojos del Salado, in the Dry Andes (5830 m a.s.l.). The results were used to investigate daily temperature fluctuations and the phase changes of water in the regolith, where the permafrost and ground ice are present. Model

A field expedition in the High Andes/Atacama Desert region revealed two types of flow-produced structures and a unique flow regime. Gullies somewhat smaller than those on Mars (width: 0.2–1 m, depth: 0.2–0.6 m, length: 4–60 m) were observed as mainly erosional structures. The other flow-related feature called infilled valleys showed activity only in specific, spatially discrete areas during the daytime

Periglacial and volcanic environments are considered terrestrial analogs of Mars with regard to astrobiological characteristics due to their specific set of extreme features. Ojos del Salado, the highest volcano on Earth (6893 m a.s.l.), is surrounded by several craters, one of which harbors the highest known altitude lake (6480 m a.s.l.), which is influenced by a rare combination of extreme environmental

Microbial ecology of permafrost, due to its ecological and astrobiological importance, has been in the focus of studies in past decades. Although permafrost is an ancient and stable environment, it is also subjected to current climate changes. Permafrost degradation often results in generation of thaw ponds, a phenomenon not only reported mainly from polar regions but also present in high-altitude

The detection of biosignatures on Mars is of outstanding interest in the current field of astrobiology and drives various fields of research, ranging from new sample collection strategies to the development of more sensitive detection techniques. Detailed analysis of the organic content in Mars analog materials collected from extreme environments on Earth improves the current understanding of biosignature

On November 5–8, 2019, the “Mars Extant Life: What's Next?” conference was convened in Carlsbad, New Mexico. The conference gathered a community of actively publishing experts in disciplines related to habitability and astrobiology. Primary conclusions are as follows: A significant subset of conference attendees concluded that there is a realistic possibility that Mars hosts indigenous microbial life

Remote sensing data are abundant, whereas surface in situ verification of atmospheric conditions is rare on Mars. Earth-based analogs could help gain an understanding of soil and atmospheric processes on Mars and refine existing models. In this work, we evaluate the applicability of the Weather Research and Forecasting (WRF) model against measurements from the Mars analog High Andes-Atacama Desert

Identifying and distinguishing between abiotic and biotic signatures of organic molecules such as amino acids and fatty acids is key to the search for life on extraterrestrial ocean worlds. Impact ionization mass spectrometers can potentially achieve this by sampling water ice grains formed from ocean water and ejected by moons such as Enceladus and Europa, thereby exploring the habitability of their

Jezero crater has been selected as the landing site for the Mars 2020 Perseverance rover, because it contains a paleolake with two fan-deltas, inlet and outlet valleys. Using the data from the High Resolution Stereo Camera (HRSC) and the High Resolution Imaging Science Experiment (HiRISE), we conducted a quantitative geomorphological study of the inlet valleys of the Jezero paleolake. Results show

Motivated by an interest in understanding the habitability of aqueous environments on Earth and in extraterrestrial settings, this study investigated the influence of ions in an artificial sodium–magnesium–sulfate–chloride ion system on the growth parameters (lag phase, growth rate, and final cell concentration) of bacteria. These four ions, in different combinations, are key components of many aqueous

The paleo-lake floor at the edge of the Jezero delta has been selected as the NASA 2020 rover landing site. In this article, we demonstrate the sequences of lake filling and delta formation and constrain the minimum life span of the Jezero paleo-lake from sedimentological and hydrological analyses. Two main phases of delta evolution can be recognized by utilizing imagery provided by the High Resolution

The last decade has witnessed a renewed interest in space exploration. Public and private institutions are investing considerable effort toward the direct exploration of the Moon and Mars, as well as more distant bodies in the solar system. Both automated and human-crewed spacecraft are being considered in these efforts. As inevitable fellow travelers on the bodies of astronauts, spaceships, or equipment

The search for spectroscopic biosignatures with the next generation of space telescopes could provide observational constraints on the abundance of exoplanets with signs of life. An extension of this spectroscopic characterization of exoplanets is the search for observational evidence of technology, known as technosignatures. Current mission concepts that would observe biosignatures from ultraviolet

As part of the Biology and Mars Experiment (BIOMEX; ILSRA 2009-0834), samples of the lichen Circinaria gyrosa were placed on the exposure platform EXPOSE-R2, on the International Space Station (ISS) and exposed to space and to a Mars-simulated environment for 18 months (2014–2016) to study: (1) resistance to space and Mars-like conditions and (2) biomarkers for use in future space missions (Exo-Mars)

Earth's ocean-atmosphere system has undergone a dramatic but protracted increase in oxygen (O2) abundance. This environmental transition ultimately paved the way for the rise of multicellular life and provides a blueprint for how a biosphere can transform a planetary surface. However, estimates of atmospheric oxygen levels for large intervals of Earth's history still vary by orders of magnitude—foremost

The Perseverance rover (Mars 2020) is equipped with an instrumental and analytical payload capable of identifying a broad range of organic molecules in geological samples. To determine the efficacy of these analytical techniques in recognizing important ecological and environmental signals in the rock record, this study utilized analogous equipment, including gas chromatography/mass spectrometry, Raman

Atmospheric organic hazes are common in planetary bodies in our solar system and likely exoplanet atmospheres as well. In addition, geochemical data support the existence of an organic haze in the early Earth's atmosphere. Much of what is known about organic haze formation derives from studies of Saturn's moon Titan. It is believed that on Titan ions play an important role in haze formation. It is

In most environments on Earth, habitable environments contain life. Experiments were conducted to investigate the decoupling of the presence of habitable conditions and life. A set of microcosms habitable for known groups of organisms, but uninhabited (i.e., uninhabited habitats), was exposed to external environmental conditions to test the hypothesis that extreme habitable environments can remain

Numerous laboratory studies of astrophysical ice analogues have shown that their exposure to ionizing radiation leads to the production of large numbers of new, more complex compounds, many of which are of astrobiological interest. We show here that the irradiation of astrophysical ice analogues containing H2O, CH3OH, CO, and NH3 yields quantities of hexamethylenetetramine-methanol (hereafter HMT-methanol;

The first organisms on the young Earth, just 1–1.5 billion years old, were likely chemolithoautotrophic anaerobes, thriving in an anoxic world rich in water, CO2, and N2. It is generally assumed that, until the accumulation of O2 in the atmosphere, life was exempted from the oxidative stress that reactive oxygen species (ROS) impose on hydrocarbon-based life. Therefore, it is perplexing to note that

Nucleobases are of significant importance to all known organisms, may be an important building block of life, and could be important biosignatures of current or past life. Given their potential significance to the field of astrobiology, it is important to understand the survival of these molecules when subjected to ionizing radiation as is present in a range of extraterrestrial environments. In this

In the past decade, astrochemistry has witnessed an impressive increase in the number of detections of complex organic molecules. Some of these species are of prebiotic interest such as glycolaldehyde, the simplest sugar, or aminoacetonitrile, a possible precursor of glycine. Recently, we have reported the detection of two new nitrogen-bearing complex organics, glycolonitrile and Z-cyanomethanimine

BioSentinel is the first biological CubeSat designed and developed for deep space. The main objectives of this NASA mission are to assess the effects of deep space radiation on biological systems and to engineer a CubeSat platform that can autonomously support and gather data from model organisms hundreds of thousands of kilometers from Earth. The articles in this special collection describe the extensive

Biology experiments in space seek to increase our understanding of what happens to life beyond Earth and how we can safely send life beyond Earth. Spaceflight is associated with many (mal)adaptations in physiology, including decline in musculoskeletal, cardiovascular, vestibular, and immune systems. Biological experiments in space are inherently challenging to implement. Development of hardware and

Analog research of human or combined human and robotic missions is an established tool to explore the workflows, instruments, risks, and challenges of future planetary surface missions in a representative terrestrial environment. Analog missions that emulate selected aspects of such expeditions have risen in number, expanded their range of disciplines covered, and seen a significant increase in their

The molecules feeding life's emergence are thought to have been provided through the hydrothermal interactions of convecting carbonic ocean waters with minerals comprising the early Hadean oceanic crust. Few laboratory experiments have simulated ancient hydrothermal conditions to test this conjecture. We used the JPL hydrothermal flow reactor to investigate CO2 reduction in simulated ancient alkaline

The aim of this study was to demonstrate for the first time Fuligo septica spore viability in the stratosphere through spore germination and its complete life cycle. These protozoan spores were flown by the National Aeronautics and Space Administration (NASA) Columbia Scientific Balloon Facility (CSBF) flight 667NT, launched from its base in Fort Sumner, New Mexico. F. septica spores were exposed to

We conducted a field test at a potential Mars analog site to provide insight into planning for future robotic missions such as Mars 2020, where science operations must facilitate efficient choice of biologically relevant sampling locations. We compared two data acquisition and decision-making protocols currently used by Mars Science Laboratory: (1) a linear approach, where sites are examined as they

Sedimentary strata on Mars often contain a mix of sulfates, iron oxides, chlorides, and phyllosilicates, a mineral assemblage that is unique on Earth to acid brine environments. To help characterize the astrobiological potential of depositional environments with similar minerals present, samples from four naturally occurring acidic salt lakes and adjacent mudflats/sandflats in the vicinity of Norseman

There has been considerable attention on how to detect life on other worlds by searching for biomolecules. However, there has been much less clarity as to when it becomes warranted to focus a mission on the search for life on another world. At a minimum, a life-detection mission should follow convincing evidence of (1) Liquid water of suitable salinity, past or present; (2) Carbon in the water; (3)

The Mars Organic Molecule Analyzer (MOMA) onboard the ExoMars 2020 rover (to be landed in March 2021) utilizes pyrolysis gas chromatography-mass spectrometry (GC-MS) with the aim to detect organic molecules in martian (sub-) surface materials. Pyrolysis, however, may thermally destroy and transform organic matter depending on the temperature and nature of the molecules, thus altering the original molecular

In the present work, near-infrared, laser-induced breakdown spectroscopy, Raman, and X-ray diffractometer techniques have been complementarily used to carry out a comprehensive characterization of a terrestrial analogue selected from the Chesapeake Bay impact structure (CBIS). The obtained data clearly highlight the key role of Raman spectroscopy in the detection of minor and trace compounds, through

The aim of the European Space Agency's ExoMars rover mission is to search for potential traces of present or past life in the swallow subsurface (2 m depth) of Mars. The ExoMars rover mission relies on a suite of analytical instruments envisioned to identify organic compounds with biological value (biomarkers) associated with a mineralogical matrix in a highly oxidative environment. We investigated

No instrument capable of direct life detection has been included on a mission payload to Mars since NASA's Viking missions in the 1970s. This prevents us from discovering whether life is or ever was present on Mars. DNA is an ideal target biosignature since it is unambiguous, nonspecific, and readily detectable with nanopore sequencing. Here, we present a proof-of-concept utilization of the Oxford

After the successful landing of the Mars Science Laboratory rover, both NASA and ESA initiated a selection process for potential landing sites for the Mars2020 and ExoMars missions, respectively. Two ellipses located in the Mawrth Vallis region were proposed and evaluated during a series of meetings (three for Mars2020 mission and five for ExoMars). We describe here the regional context of the two

Sulfate and iron oxide deposits in Río Tinto (Southwestern Spain) are a terrestrial analog of early martian hematite-rich regions. Understanding the distribution and drivers of microbial life in iron-rich environments can give critical clues on how to search for biosignatures on Mars. We simulated a robotic drilling mission searching for signs of life in the martian subsurface, by using a 1m-class

The biological risks of the deep space environment must be elucidated to enable a new era of human exploration and scientific discovery beyond low earth orbit (LEO). There is a paucity of deep space biological missions that will inform us of the deleterious biological effects of prolonged exposure to the deep space environment. To safely undertake long-term missions to Mars and space habitation beyond

Extreme factors such as space microgravity, radiation, and magnetic field differ from those that occur on Earth. Microgravity may induce and select some microorganisms for physiological, metabolic, and/or genetic variations. This study was conducted to determine the effects of simulated microgravity conditions on the metabolism and gene expression of the probiotic bacterium Lactobacillus reuteri DSM17938

A long-term goal of exoplanet studies is the identification and detection of biosignature gases. Beyond the most discussed biosignature gas O2, only a handful of gases have been considered in detail. In this study, we evaluate phosphine (PH3). On Earth, PH3 is associated with anaerobic ecosystems, and as such, it is a potential biosignature gas in anoxic exoplanets. We simulate the atmospheres of habitable

Samples that are likely to contain evidence of past life on Mars must have been deposited when and where environments exhibited habitable conditions. Mars analog sites provide the opportunity to study how life could have exploited such habitable conditions. Acidic iron- and sulfur-rich streams are good geochemical analogues for the late Noachian and early Hesperian, periods of martian history where

Models of Titan predict that there is a subsurface ocean of water and ammonia under a layer of ice. Such an ocean would be important in the search for extraterrestrial life since it provides a potentially habitable environment. To evaluate how Earth-based proteins would behave in Titan's subsurface ocean environment, we used molecular dynamics simulations to calculate the properties of proteins with

In ecology and conservation biology, the concept of assisted evolution aims at the optimization of the resilience of organisms and populations to changing environmental conditions. What has hardly been considered so far is that this concept is also relevant for future astrobiological research, since in artificial extraterrestrial habitats (e.g., plants and insects in martian greenhouses) novel environmental

This work aims at addressing whether a catastrophic failure of an entry, descent, and landing event of a Multimission Radioisotope Thermoelectric Generator-based lander could embed the heat sources into the martian subsurface and create a local environment that (1) would temporarily satisfy the conditions for a martian Special Region and (2) could establish a transport mechanism through which introduced