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Age‐related changes in testicular histological structure, testosterone level, libido and semen traits in male Coturnix quail Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-12 Mohamed Roshdy, Ahmed M. Hanafy, Hassan A. Khalil, Salwa M. Siam, Mohamed E. Abd El‐Hack, Ayman A. Swelum, Gamaleldin M. Suliman, Vincenzo Tufarelli, Mohamed E. Mady
To investigate the effect of age in male quail on testicular weight and histology, sexual libido and semen characteristics, a study was performed on 100 quails at 10, 16, 22, 28 and 34 weeks of age. The body and testicular weights were significantly (p < .05) higher at 16 and 22 than at 28 weeks of age. The circumference and diameter of the seminiferous tubules were significantly (p < .05) higher at
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Evaluation of Kappa‐carrageenan supplementation in extender for post‐thaw Kajli ram sperm quality Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-11 Saima Qadeer, Asma Ashraf, Muhammad Umer Farooq, Sana Ullah, Muhammad Asad, Ahmad Muneeb, Muhammad Adil
Cryopreservation is one of the reliable techniques for long‐term storage of sperm. The success of this technique depends on the choice of cryoprotectant; therefore, a plethora of literature has reported the effects of different cryoprotective agents so far. Kappa‐carrageenan (κ‐carrageenan) is a hydrocolloid polysaccharide extracted from red marine seaweed. Its unique property makes it a promising
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Epididymitis with Pasteurella multocida isolated from two male Japanese Black beef calves Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-11 Chihiro Kanno, Shogo Sato, Yosuke Maeda, Ryo Ando, Chun‐Ho Park, Hiroaki Kawaguchi, Taishi Tanabe, Fumiaki Takahashi
Two male Japanese Black calves developed an enlarged scrotum and testis. Orchiectomy was performed and pus was collected during surgery. After removal of the testis, bacteriological and histopathological examinations were conducted to investigate the cause and confirm the diagnosis. Based on the results obtained, both cases were diagnosed with epididymitis caused by an infection with Pasteurella multocida
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Fertilization with follicular fluid reduces HSP70 and BAX expression on bovine in vitro embryos Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-09 Bruno Porpino Homobono, Marcela Oliveira das Mercês, Leonardo Henrique da Silva Nogueira, Eduardo Baia de Souza, Ana Paloma Lobo Cardoso, Ana Beatriz Sousa Santos, Anelise de Sarges Ramos, Matheus Hadigo Pires Costa, Priscila Di Paula Bessa Santana, Nathalia Nogueira da Costa de Almeida, Marcela Silva Cordeiro, Simone do Socorro Damasceno Santos
The in vivo fertilization process occurs in the presence of follicular fluid (FF). The aim of this study was to evaluate the effect of in vitro fertilization medium supplementation with 5% or 10% bovine follicular fluid (BFF) on the production of in vitro bovine embryos. FF was collected from ovarian follicles with a diameter of 8–10 mm, and cumulus–oocyte complexes (COCs) were co‐incubated with sperm
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Melatonin improves the viability and ultrastructure of bovine oocyte‐granulosa complexes of in vitro cultured early antral follicles Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-09 Bianca R. Silva, Danisvânia R. Nascimento, Francisco C. Costa, Antônia V. Azevedo, Laís R. F. M. Paulino, Francisco L. N. Aguiar, Ana L. P. S. Batista, Mariana A. M. Donato, José R. V. Silva
This study aims to investigate the effects of melatonin on follicular growth, viability and ultrastructure, as well as on the levels of mRNA for antioxidant enzymes, reactive oxygen species (ROS) and meiotic progression in oocytes from in vitro cultured bovine early antral follicles. To this end, isolated early antral follicles (500–600 μm) were cultured in TCM‐199+ alone or supplemented with 10−6
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Effects of Leonurine on oocyte maturation and parthenogenetic embryo development in sheep Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-05 Panpan Wei, Jing Wang, Hengbin Yu, Yan Chen, Chang Liu, Yue Zhang, Weibin Zeng, Guangdong Hu
Leonurine (LEO), an alkaloid isolated from Leonurus spp., has anti‐oxidant, anti‐inflammatory and anti‐apoptotic effects and can prevent damage caused by reactive oxygen species (ROS). These properties suggest that it can improve the maturation rate of oocytes and developmental ability of embryos, which are key parameters in animal breeding. In this study, the effects of LEO on in vitro maturation
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Evaluation of a commercial proAKAP4 kit for the assessment of fresh and frozen–thawed feline spermatozoa Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-04 Sylwia Prochowska, Maria Eberhardt, Wojciech Niżański
ProAKAP4 is gaining increasing attention as a potential marker of semen quality in many species, but while there is a commercial kit for assessing proAKAP4 in the domestic cat, there are no publications about its use. The aim of this study was to evaluate the commercial proAKAP4 kit – Cat 4MID® Kit (SPQI – 4BioDx, Lille, France) for the assessment of feline semen. Semen was collected from 54 male cats
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Analysing the genetic diversity and population structure of five native Turkish cattle breeds using SNP data Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-01 Mervan Bayraktar, Zeynel Cebeci, Gökhan Gökçe
The conservation and sustainable utilization of cattle genetic resources necessitate a comprehensive understanding of their genetic diversity and population structure. This study provides an analysis of five native Turkish cattle breeds: Anatolian Black (ANB), Turkish Grey (TUR), Anatolian Southern Yellow (ASY), East Anatolian Red (EAR), and South Anatolian Red (SAN) using 50 K SNP data. These breeds
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Dystocia frequency and causes in horses with pregnancy disorders or a history of dystocia: A prospective study Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-01 Markus Ellerbrock, Judith Krohn, Kathrin Büttner, Axel Wehrend
Dystocia typically presents a life‐threatening condition for both the mare and the foal. This prospective long‐term study aimed to ascertain whether mares with prior pregnancy disorders or a history of dystocia were at a higher risk of experiencing subsequent dystocia in comparison to those without such medical antecedents. To achieve this goal, the authors analysed 207 parturitions and 164 mares over
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The effect of human menopausal gonadotropin and equine chorionic gonadotropin on the reproductive performance of treated ewes with short‐term progesterone injections and sponge during the non‐breeding season Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-03-01 Shahram Dehghani Ghale Sorkh, Abdolah Mirzaei, Abbas Zeyghami
This study investigated the effect of human menopausal gonadotropin (hMG) on reproductive efficiency of synchronized ewes with the sponge and progesterone (P4) injection‐based protocols. In study 1, anoestrous ewes (n = 120) were used. Sixty ewes were treated with sponge (S) for 12 days. The injection of eCG (SeCG group, n = 30) or hMG (ShMG, n = 30) was given at the time of sponge removal. Thirty
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Anti-Müllerian hormone: A novel biomarker for detecting bovine freemartinism Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-02-17 Davut Koca, Yavuz Nak, Sait Sendag, Deniz Nak, Ali Osman Turgut, Talha Avcılar, Zeynep Merve Ekici, Nebi Cetin, Kemal Bagci, Ahmet Aktar, Hakan Sagirkaya, Selim Alcay, Axel Wehrend
The anti-Müllerian hormone (AMH) indicates ovarian reserve in cattle, maintaining a consistent trajectory post-puberty. In heterosexual pregnancies, the development of the Müllerian duct in female foetuses is inhibited, resulting in an anticipated minimal or absent ovarian reserve capacity. This investigation aimed to compare AMH levels in healthy Holstein heifers that had reached puberty with those
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The effect of alfalfa supplementation during the peripartum period on udder oedema and fertility in dairy sheep Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-02-04 Gokhan Bozkurt, Feyyaz Kaya, Ibrahim Tasal, Ramazan Yildiz
In recent years, there has been a trend towards hyperoestrogenic feeding methods in field conditions, particularly in dairy small ruminants. This study aimed to investigate the effect of alfalfa supplemented ration in the prepartum period on the occurrence of udder oedema in dairy ewes and its impact on fertility problems (dystocia, vaginal prolapse, uterine prolapse, retained placenta) and diseases
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Positive effects of rutin on female reproduction Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-02-04 Alexander V. Sirotkin
This article reviews the source and properties of rutin (vitamin P), its general physiological and medicinal effects and their mechanisms, but the main subject of it is the currently available knowledge concerning the character and mechanisms of action of rutin on female reproductive processes. The available data demonstrate the stimulatory action of rutin on female reproductive processes: it can promote
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Catalase and superoxide dismutase supplementation to the diluent for cryopreservation of collared peccary semen Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-31 Samara Sandy Jerônimo Moreira, Romário Parente dos Santos, Andréia Maria da Silva, Tayná Moura Matos, Andreza Vieira Brazil, João Batista Freire Souza-Júnior, José Rodolfo Lopes de Paiva Cavalcanti, Alexsandra Fernandes Pereira, Alexandre Rodrigues Silva
We evaluated the effects of supplementation with catalase (CAT) and superoxide dismutase (SOD) antioxidants, isolated or combined, on the freezability of collared peccary sperm. Semen aliquots from ten individuals were diluted in Tris-yolk with or without the presence of antioxidants CAT (200 or 400 IU/mL) and SOD (150 or 300 IU/mL) alone or in combination (CAT: 200 IU/mL + SOD: 150 IU/mL), and then
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Evaluation of parity and its interaction effect with season of calving, period of calving on production and reproduction traits in Hardhenu (Bos taurus × Bos indicus) dairy cattle Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-31 Pushpa, Ankit Magotra, Vikas Sindhu, Pradeep Chaudhary
This study investigated the impact of parity, period of calving, and season of calving on various production and reproduction traits in Hardhenu cattle. Third-parity cows outperformed first and second-parity cows, exhibiting significantly higher total milk yield (TMY), 305 days milk yield (305d MY), peak yield (PY), and artificial insemination per conception (AIPC) (p < .01). The Lactation length (LL)
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Improved the in vitro blastocyst development in oocytes recovered from cyclic buffaloes Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-22 Muhammad Usman Mehmood, Amjad Riaz, Muhammad Shahzad, Zahid Naseer, Kehuan Lu, Yangqing Lu
This study examined the impact of cyclicity (with or without cycle corpus luteum; CL) on oocyte quality and embryonic development in buffaloes. We collected oocytes from the ovaries of slaughtered buffaloes (N = 158 cyclic; n = 316 ovaries and N = 177 acyclic; n = 353 ovaries). Blood progesterone concentration and number of oocytes per ovary were higher in cyclic buffaloes. Cyclic buffalo ovaries produce
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Kisspeptin stimulates oestradiol biosynthesis by upregulating steroidogenic transcripts and proliferation markers in the bubaline granulosa cells in vitro Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-19 Renu Sharma, Manas Kumar Patra, Thejaswini Meda Puttanarsappa, Hitesh, Mohammad Rehan Ahmad Raza, Tarun Kumar Sahu, Karikalan Mathesh, Zunjar Baburao Dubal, Subrata Kumar Ghosh, Gyanendra Kumar Gaur, Goutam Kumar Das, Sanjay Kumar Singh, Narayanan Krishnaswamy
Kisspeptin (Kp), an upstream regulator of GnRH release, is essential for the development and function of reproductive axis. Previously, we demonstrated the localization of Kp and its receptor (Kiss1r) in the active follicle in the bubaline ovary. Present study aimed to determine the effect of Kp on granulosa cell (GCs) functions, especially oestradiol (E2) and progesterone (P4) production, and differential
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Exploring MicroRNA biogenesis, applications and bioinformatics analysis in livestock: A comprehensive review Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-19 Komal Jaglan, S. S. Dhaka, Ankit Magotra, C. S. Patil, Amandeep Ghanghas
Small non-coding RNAs called microRNAs (miRNAs) control the expression of genes post-transcriptionally. Their correlation with commercial economic traits including milk, meat and egg production, as well as their effective role in animal productivity, fertility, embryo survival and disease resistance, make them significant in livestock research. The miRNAs exhibit distinct spatial and temporal expression
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Development of alternative diagnosis of HH1, HH3, HH5 and HCD fertility haplotypes and subfertility syndrome in cattle Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-19 Marzhan Shormanova, Abzal Makhmutov, Aizhamal Shormanova, Zhadyra Muslimova, Yessengali Ussenbekov
The increasing prevalence of hereditary anomalies in Holstein cattle populations presents a pressing issue, leading to concerns such as embryonic mortality and the birth of non-viable offspring. This study addresses the urgency of managing harmful genetic mutations in Holstein cattle by developing alternative diagnostic methods. The research aims to devise effective means to diagnose fertility haplotypes
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Morphology and morphometry of sperm in Kurdish stallions, a local breed from western Iran Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-19 Armaghan Nooroozian, Nader Goodarzi, Rohollah Dehghani Tafti
The present work was designed for a thorough investigation into the sperm morphology and morphometry of Kurdish stallions. The semen samples were collected from 10 Kurdish stallions. Three preparations from each ejaculate were stained with eosin–nigrosin (EN), Diff-Quik (DQ) and Rose Bengal (RB). The area, perimeter, length and width of the sperm head as well as tail length and total sperm length were
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Insights into alternative cryoprotectants to freeze sperm of domestic cats Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-18 Maria Isabel Mello Martins, Vinícius Wagner Silva, Myrian Megumy Tsunokawa Hidalgo, Alain Fontbonne
Globalization and habitat destruction pose a significant threat to wildlife felids. Even though conservation banks for genetic materials have been created, the sperm cryopreservation with minimal cell damage is still a great challenge. Thus, this study aimed to compare the effects of two commercial extenders with different concentrations of alternative cryoprotectants on thawed sperm quality of domestic
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Evaluation of pharmacological alternatives to reduce the pain and discomfort produced by electroejaculation in rams Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-12 Mariana Karla Francolino da Silva, Leonardo de Almeida Gélio, Eunice Oba, Douglas Anderson de Freitas, Renata Elisa Green, Marcílio Nichi, André Maciel Crespilho
Electroejaculation (EE) represents the main technique for semen collection from domestic and wild animals independently of libido. However, the technique is associated with intense involuntary muscle contractions, vocalization, ataxia and lying down, caused by the electric stimulation of the nerves in the caudal epigastric region. These clinical manifestations represent important indicators of discomfort
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Evaluation of blood minerals and oxidative stress changing pattern in prepartum and postpartum Achai and Holstein Friesian dairy cows Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-08 Fahad Gul, Hassan Amin, Shabana Naz, Muhammad Tahir Khan, Ibrahim A. Alhidary, Rifat Ullah Khan, Gianluca Pugliese, Vincenzo Tufarelli
This research focused on evaluating blood mineral levels and malondialdehyde (MDA) as an indicator of oxidative stress before and after giving birth in Achai and Holstein Friesian (HF) dairy cows. Blood samples were obtained from a total of 50 cows representing both breeds on the third, second, and first week prior to calving, as well as on the day of calving (day 0). Subsequently, samples were collected
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Estimation of direct and maternal covariance along with genetic and phenotypic trends of reproduction traits in Murrah buffalo Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-08 Smriti Sharma, Surender Singh Dhaka, Chandrashekhar Santosh Patil, Pallavi Rathi
The research utilized data from 662 Murrah buffaloes meticulously recorded over 24 years (1996–2019) from historical pedigree sheets maintained at the buffalo farm of the Department of Livestock Production and Management (LPM) at Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), Hisar. A series of six univariate animal models were employed to calculate estimates of (co)variance
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Analysis of histone H3K4me3 modifications in bovine placenta derived from different calf-production methods Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-09 Kanoko Hattori, Yoichiro Hoshino, Masayuki Kachi, Yasumitsu Masuda, Satoshi Yamamoto, Shinnosuke Honda, Naojiro Minami, Shuntaro Ikeda
In ruminants, the overgrowth of offspring produced by in vitro fertilization (IVF) is a common problem. Abnormal epigenetic modifications caused by environmental factors during the early embryonic period are suspected as an aetiology of overgrowth. In this study, we investigated the genome-wide histone H3K4me3 profiles of bovine placentae that play a pivotal role in foetal development and compared
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Serum anti-Müllerian hormone around the time of ovulation simulated by exogenous hormones in clouded leopards (Neofelis nebulosa) Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-04 Sittat Chumsri, Itti Boonorrana, Junpen Suwimonteerabutr, Wanlaya Tipkantha, Ampika Thongphakdee, Kaywalee Chatdarong
Anti-Müllerian hormone (AMH) is produced by granulosa cells of the antral follicles. It serves as a promising biomarker for ovarian reserve and responsiveness to ovarian stimulation in humans and domestic animals. This study aimed to validate the AMH Gen II enzyme-linked immunosorbent assay (ELISA) and correlate ovarian structures with serum AMH concentrations after stimulation treatment in clouded
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Quality and redox state of bovine sperm cryopreserved with resveratrol use of resveratrol in bovine semen Reprod. Domest. Anim. (IF 1.7) Pub Date : 2024-01-04 Felipe Correa, Esteban Ceballos, Benjamín Rojano, Giovanni Restrepo, Alexandra Usuga
The use of antioxidants for semen preservation prevents oxidative damage caused by reactive oxygen species (ROS). One of the most promising natural antioxidants is resveratrol, a phytoalexin derived from plants, grapes, berries, peanuts and red wine. To evaluate the effect of resveratrol on the quality and redox status of cryopreserved bovine semen. Five bulls were subjected to electroejaculation to
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Development of porcine embryos cultured in media irradiated with ultraviolet-C Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-27 Nanaka Torigoe, Megumi Nagahara, Suong Thi Nguyen, Qingyi Lin, Koki Takebayashi, Bin Liu, Mutsumi Aihara, Masayasu Taniguchi, Takeshige Otoi
Sterilization of the culture medium using ultraviolet (UV)-C reduces the potential adverse effects of microorganisms and allows for long-term use. In the present study, we investigated the effects of a medium directly irradiated with UV-C prior to in vitro culture on the development and quality of porcine in vitro-fertilized embryos and the free amino acid composition of the culture media. The culture
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Renal biomarkers, clinical parameters, and renal Doppler velocimetry in bitches with cystic endometrial hyperplasia–pyometra complex Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-27 Leticia Amanda dos Santos Silva, Luiz Guilherme Corsi Trautwein, Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Julia Rodrigues Greghi, Natália Ribeiro Sambatti, Vinícius Wagner Silva, Karina Keller Marques da Costa Flaiban, Maria Isabel Mello Martins
Cystic endometrial hyperplasia (CEH)–pyometra complex is the most common uterine infection in adult and elderly bitches and can cause renal dysfunction. The aim of this study was to measure and compare urinary creatinine, urea, symmetric dimethylarginine (SDMA), urinary protein–creatinine ratio (UPC), measurement of systolic blood pressure (SBP), and Doppler velocimetry of renal arteries in patients
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Coenzyme Q10 improves the quality and in vitro fertility of post-thawed buffalo (Bubalus bubalis) semen via its antioxidative effect Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-26 Hussain Ahmed, Muhammad Umar Ijaz, Sarwat Jahan, Mehreen Riaz, Haney Samir, Ayman A. Swelum
This study aimed to determine the effects of Coenzyme Q10 (CoQ10) in the freezing medium on functional and oxidative stress parameters and in vitro fertilization (IVF) rate of buffalo sperm. Collected samples were relocated to the laboratory for initial evaluation, gentle dilution in extenders, cooling (4°C, 2 h), equilibration (4°C, 4 h), packaging (straws, 0.5 mL), programmable freezing, and thawing
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Determination of expression patterns of miR-26a, and preimplantation factor levels for early pregnancy detection in nulliparous and multiparous cows Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-26 Kemal Tuna Olğaç, Murat Onur Yazlik, Hüseyin Özkan, Ufuk Kaya, Mehmet Borga Tirpan
For maximum productivity in a dairy farm, the earliest and the most accurate detection of pregnancy is essential. The aim of this study was to determine the efficacy of expression patterns of miR-26a, and serum Preimplantation Factor (PIF) levels for pregnancy diagnosis during the early pregnancy in nulliparous and multiparous cows. A total of 60 cows (30 nulliparous and 30 multiparous Holstein cows)
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Ameliorative effect of crocin on post-thaw quality, fertility-associated gene expression and fertilization potential of buffalo (Bubalus bubalis) bull sperm Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-21 Ghulam Shabbir Khan, Muhammad Zahid Tahir, Muhammad Yasir Zahoor, Hifz-ul- Rahman, Amjad Riaz
Buffalo bull sperm suffer more cryoinjuries due to lipid peroxidation of high structural polyunsaturated fatty acid contents than cattle sperm. Consequently, the post-thaw fertilization potential of buffalo bull sperm is compromised. Crocin is a carotenoid known for its antioxidant potential through scavenging reactive oxygen species. Objectives of the current study were to investigate the effect of
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Histological study of Bactrian camel cryptorchidism and expression of immunoglobulin λ light chain in the testicular and epididymis of cryptorchid Bactrian camel Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-09 Dapeng Yang, Ligang Yuan, Xiaojie Ma, Yumei Qi, Shaoyu Cheng, Yong Zhang
Lambda light chains (λ-LCs) are frequently responsible for triggering the activation of inflammatory factors in autoimmune disorders, and an increase in their levels will cause various pathological changes in serum. The aim of this study was to determine the histological differences between the epididymis and testis of normal and cryptorchid Bactrian camels and the differences in λ-LC expression in
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Exploring testicular miRNA profiles during developmental stages in Dezhou donkeys: A preliminary insight Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-07 Zulfiqar Ahmed, Wei Liu, Jie Yu, Hong Dong, Zahid Naseer, Imtiaz Ahmad, Imran Ahmed, Xijun Wang
Testicular development and spermatogenesis are complex phenomena controlled by various genetic factors, including miRNA-based post-transcriptional gene expression regulation. Exploring the miRNA expression patterns during testicular development in Dezhou donkeys would enhance our understanding of equine fertility and spermatogenesis. In this investigation, we examined the testicular miRNA profiles
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Pregnancy and birth rate outcomes in alpacas (Vicugna pacos) inseminated with frozen semen using two commercial extenders Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-06 M. Contreras Huamani, C. A. Olaguivel Flores, C. Y. Guillen Palomino, M. I. Carretero, F. G. Fumuso, I. M. Laines Arcce
The aim of this study was to evaluate alpaca pregnancy outcomes and birth rates of females inseminated with frozen semen using two commercial extenders. A total of 18 ejaculates from 8 adult alpaca males were obtained with artificial vagina, and macroscopic and microscopic semen characteristics were assessed. Afterwards, samples were divided into two aliquots, diluted with Biladyl® B or AndroMed®,
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Effects of long-acting injectable progesterone supplementation at early dioestrus on pregnancy maintenance in beef and dairy recipient cattle Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-01 Adomar Laurindo Neto, Felipe Lopes Ruas, Bruno Silva Espírito Santo, Ana Clara Degan Mattos, Júnior Borges Silva, Marcos Flávio Diniz Júnior, Danilo Fonseca Cardoso Pereira, Izabelle Pereira Lacerda, José Oliveira Carvalho, Guilherme Pugliesi
We tested in the present study the hypothesis that supplementation with long-acting P4 (iP4) at different times of the initial dioestrus improves pregnancy rates in dairy and beef recipients submitted to fixed-time embryo transfer (FTET). Recipients from commercial farms had their oestrous cycle synchronized with an E2/P4-based protocol in three experiments (Exp. 1 to 3). In Exp. 1, dairy heifers (n = 76)
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Reduction of cryopreservation-induced structural, functional and molecular damages in ram sperm by hydrated C60 fullerene Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-12-01 İbrahim Halil Güngör, Gaffari Türk, Serap Dayan Cinkara, Tutku Can Acisu, Ahmet Tektemur, Deniz Yeni, Fatih Avdatek, Gözde Arkali, Recep Hakkı Koca, Şeyma Özer Kaya, Meltem Sagiroglu, Ebru Etem Önalan, Mustafa Sönmez, Seyfettin Gür, Abdurrauf Yüce
This study aimed to investigate the morphological, functional and molecular changes in frozen–thawed ram sperm using an extender containing different concentrations of hydrated carbon 60 fullerene (C60HyFn), a nanotechnological product. Semen taken from each of the seven Akkaraman rams were pooled. Semen collection was done twice a week and it continued for 3 weeks. Each pooled semen sample was divided
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Influence of nano-formulations of clove bud (Syzygium aromaticum) extract on freezing ability, antioxidant capacity, caspase-3 activity, acrosome reaction and fertility of frozen rabbit semen Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-29 Wael A. Khalil, Amr M. I. Atta, Ibrahim T. El-Ratel, Sameh A. Abdelnour, Abdel-Khalek E. Abdel-Khalek, Sara F. Fouda
The current study aimed to investigate the impact of nano-formulations of clove bud ethanolic extract (CBENF) in the extender on sperm characteristics, antioxidant capacity, oxidative biomarkers, enzymatic activity, apoptosis and fertility of post-thawed rabbit semen. Twelve mature male rabbits semen samples were pooled and cryopreserved in a Tris-egg yolk-based extender containing varying concentrations
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Motility, oxidative status and morphology of frozen-thawed bovine semen are not impacted by fatty acid exposure in vitro Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-28 Camila Cupper Vieira, Daniele Missio, Daniela dos Santos Brum, Rafaela Dalmolin Menezes, Francielli Weber Santos Cibin, Fernando Silveira Mesquita, Paulo Bayard Dias Gonçalves, Rogério Ferreira
While sperm migrate within the reproductive tract of cows experiencing negative energy balance (NEB), they come into contact with elevated concentrations of non-esterified fatty acids (NEFA). For this reason, this study aimed to investigate the effects of three different NEFA – palmitic acid (PA), stearic acid (SA), and oleic acid (OA) – on bovine sperm motility, kinetic parameters, oxidative status
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Optimizing selection strategy for enhancing reproduction efficiency in Indian crossbred goats using milk productivity as a selection criterion Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-27 Amritanshu Upadhyay, Rani Alex, Shweta Sahoo, Kashif Dawood Khan, Pradyut Das, Mahesh Shivanand Dige, Vikas Vohra, Gopal Ramdasji Gowane
The present study was aimed at optimizing the selection strategy for enhancing reproductive efficiency and milk productivity of Alpine × Beetal crossbred goats. The data set included 2949 milk trait records across parities and 1389 milk records from first parity and corresponding reproductive traits. The traits included for analysis were 150-day milk yield (150DMY), days in milk (DIM), peak yield (PY)
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Bovine congenital defects recorded by veterinary practitioners Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-17 J. F. Mee, D. Murphy, M. Curran
A mobile phone app was used by 59 veterinary practitioners to collect case histories and images of 191 cattle with congenital defects distributed nationally over a 3-year period. The majority of cases were recorded during the spring calving season (57.6%) in pluriparous dairy dams. The majority of calves were recorded at birth or within the first week (66.5%) in singletons born at full-term. On the
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Academics meet practitioners: Interactive exchange instead of consuming knowledge on dairy calving management Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-16 John F. Mee, Otto Szenci, Carola Fischer-Tenhagen
Consensus on optimal calving management is difficult to achieve among veterinarians. Some of this discordance may stem from asymmetry between the literature written by subject matter experts (SME) and the knowledge, attitude and practice (KAP) of non-SMEs and veterinary practitioners. Hence, the objective of this study was to assess how veterinary practitioners KAPs in calving management compared with
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Bicarbonate and BSA increase the capacitation pattern and acrosomal exocytosis in boar sperm after 120 min of incubation Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-14 Sylwia Dudkiewicz, Patricia Peris-Frau, Helena Nieto-Cristóbal, Julián Santiago-Moreno, Eduardo de Mercado, Manuel Álvarez-Rodríguez
Sperm capacitation is a crucial step towards the acquisition of fertilizing capacity. Despite the attempts to mimic the in vivo situation, there is still a lack of standardization in vitro techniques. Bicarbonate and serum albumin (BSA) are routinely used, although controversial results are reported regarding the optimal concentration of each compound. In addition, whether caffeine is needed on in vitro
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Clinical applicability of external and internal body dimensions in predicting dystocia in late-gestation Holstein-Friesian heifers Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-13 Zenon Nogalski, Dariusz Piwczyński, Anna Nogalska
The objective of this study was to determine the clinical applicability of maternal intrapelvic area (PA) and selected morphometric parameters that can be measured before parturition in predicting dystocia in dairy heifers. The measurements were performed in 374 late-gestation Holstein-Friesian heifers. Inner pelvic height and width were measured using a pelvimeter, and PA was calculated. The heifers
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Intra-uterine programming of future fertility Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-09 Pascale Chavatte-Palmer, Anne Couturier-Tarrade, Delphine Rousseau-Ralliard
The developmental origins of health and disease (DOHaD) shows that a relationship exists between parental environment at large, foeto-placental development and the risk for the offspring to develop non-transmittable disease(s) in adulthood. This concept has been validated in both humans and livestock. In mammals, after fertilization and time spent free in the maternal reproductive tract, the embryo
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Supplementation of media with gamma-oryzanol as a novel antioxidant to overcome redox imbalance during bovine oocyte maturation in vitro Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-09 Najmeh Davoodian, Ali Kadivar, Hossein Mehrban
This study evaluated the effect of supplementing IVM media with γ-oryzanol (ORY), a nutraceutical derived from rice bran oil, on the development of bovine oocytes and hindering the compromising effect of redox imbalance. An in vitro model of the bovine cumulus-oocyte complex was used for the evaluation of nuclear maturation and development. Antioxidant activity was investigated by assessing the level
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Improving chilled and frozen buck sperm characteristics by adding melatonin and L-carnitine to the preservation medium Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-09 Ibrahim S. Abd El-Hamid
This study evaluated the effects of melatonin (MLT) and L-carnitine supplementation on sperm quality and antioxidant capacity during chilled and cryopreservation. Twenty-four ejaculates were collected from six Damascus bucks, 4 ejaculates each, from mid-September to mid-October 2022. The pooled semen from each collecting session was divided into 5 equal aliquots after being diluted (1:10) with Tris-citric
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Effect of recombinant bovine somatotropin on the reproductive efficiency of beef cows subjected to differently timed-artificial insemination protocols Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-05 Grassiele Gasseferth, Francisco Romano Gaievski, Tácia Gomes Bergstein-Galan, Adalberto Gassenferth Júnior, Angelo Bragato, Vitor Mohad Valle, Gabriela Volf Andrade Rosa, Fernando Andrade Souza, Marina de Pauli Thomaz, Luiz Ernandes Kozicki
This study aimed to verify the reproductive efficiency of beef cows treated with recombinant bovine somatotropin (rbST). Study 1, Bos indicus cows were distributed (three groups). The control group (CG) was subjected: on day zero (d0), the animals received a CIDR and oestradiol benzoate (EB); on (d8, CIDR was removed, and PGF2α and oestradiol cypionate (EC) were administered; on d10, timed Artificial
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Follistatin (FST) is expressed in buffalo (Bubalus bubalis) ovarian follicles and promotes oocyte maturation and early embryonic development Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-02 Haoxin Wang, Weili Chen, Penglei Shen, Yun Feng, Deshun Shi, Fenghua Lu
Follistatin (FST), a member of the transforming growth factor-β (TGF-β) superfamily, has been identified as an inhibitor of follicle-stimulating hormone. Previous studies showed that it plays an important role in animal reproduction. Therefore, this study aims to investigate its effect on the maturation of buffalo oocytes in vitro, and the underlying mechanism of FST affecting oocyte maturation was
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Association between levels of insulin-like growth factor-1 in serum and seminal plasma with fresh and frozen-thawed semen characteristics in Beetal bucks Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-02 Keshav Kumar, Mitali Dutta, Sourabh Deori, Sayed Nabil Abedin, Mohua Das Gupta, Sudip Sinha, Arijit Shome, Soihem Diana Rongmei, Shantanu Tamuly, Girin Hazarika, Pranjal. Borah
The aim of this study was to investigate the relationship between the levels of insulin-like growth factor-1 (IGF-1) in serum and seminal plasma and the characteristics of semen in Beetal bucks (Capra hircus). A total of 12 adult Beetal bucks were involved in the study, with each buck providing six ejaculates collected using a standard artificial vagina (n = 72 total). Only qualified semen samples
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Polymorphism of ADAM12, DPP6 and PRKN genes and their associations with milk production traits in Holstein Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-02 Rui-Rui Li, Hong-hong Hu, Xue Feng, Chun-li Hu, Yan-fen Ma, Bei Cai, Li-Yun Han, Yun Ma
Milk production traits as the most important economic traits of dairy cows, they directly reflect the benefits of breeding and the economic benefits of pasture. In this study, A disintegrin and metalloproteinase-12 (ADAM12), Parkinson's disease gene 2 (PRKN) and dipeptidyl peptidase-like protein subtype 6 (DPP6) polymorphism in 384 Chinese Holstein cows were detected by time-of-flight mass spectrometry
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Factors affecting birth weight and stillbirth in sows housed in a tropical environment Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-11-01 Yosua Kristian Adi, Rafa Boonprakob, Roy N. Kirkwood, Padet Tummaruk
This study analysed data from a commercial swine herd in Thailand equipped with a free-farrowing housing system, comprising 17,196 piglets from 1318 litters, to explore the impact of sow and litter characteristics on the piglet birth weight and the incidence of stillbirth. The piglets were classified into four groups based on the total number of piglets born per litter (TB): ≤9 (n = 1434), 10–12 (n = 3232)
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Association between maternal growth in the pre-conception and early gestational period of nulliparous dairy heifers with anti-Müllerian hormone in their female offspring Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-10-30 Eilidh E. Thomson, Marijke E. Beltman, Mark A. Crowe, Conor G. McAloon, John G. Furlong, Mary M. Duane, Jane P. N. Brennan, Michael M. McDonald, Emmet T. Kelly
Anti-Müllerian hormone (AMH) is secreted by granulosa cells of healthy, growing follicles and is positively correlated with the ovarian reserve. Maternal and environmental factors, such as nutrition, disease, parity and endocrine disruptors, are thought to have a profound impact on ovarian reserve development during early foetal life. For genetic progress, it can be advantageous to breed dairy replacements
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Influence of the coincubation of post-thawed bull semen with elevated β-hydroxybutyrate concentrations on sperm characteristics Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-10-27 A. Abdelli, D. Raboisson, M. Besbaci, I. Belabdi, A. Kalem, R. Kaidi, A. Johannisson, J. M. Morrell, M. Iguer-Ouada
In this study, the relationships between post-thaw bull sperm characteristics and hyperketonemic conditions after coincubation with cow plasma or media were determined to investigate if such a condition could affect bull sperm characteristics. Two experiments were conducted. In experiment 1, blood samples were collected from 31 cows to prepare plasma. Cows were independently categorized into two groups
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Exploring uterine inflammation in postpartum primiparous precocious and conventional and multiparous Bos indicus beef cows Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-10-27 Jéssica de Souza Andrade, Elizângela Mírian Moreira, Vanessa Lemos de Souza, Ingrid Pedraça Barbosa, George Moreira Silva, Leonardo Silva Gomes, Samira Alves de Souza Silva, Gabrielly Cristina Santos Noleto, Renata Reis da Silva, Uriel Secco Londero, Márcio Nunes Correa, Luiz Francisco Machado Pfeifer
This study aimed to compare the postpartum uterine dynamics of primiparous precocious (PP), primiparous conventional (PC) and multiparous conventional (MC) Bos indicus beef cows. For this purpose, PP (n = 8), PC (n = 18) and MC (n = 12) cows were enrolled in this study. These cows were evaluated at 20 and 10 days prepartum and weekly from parturition to 42 days postpartum (DPP). During this period
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Uterine prolapse in a non-pregnant bitch Reprod. Domest. Anim. (IF 1.7) Pub Date : 2023-10-27 Marie Sophie Greiling, Eva-Maria Packeiser, Ingo Gerhauser, Sandra Goericke-Pesch
A ten-year-old mixed breed bitch was presented for a tissue prolapse protruding from her vulva. Following detailed examination and stabilization, the ovaries and uterine horns were removed by laparotomy, whereas the prolapsed tissue identified as uterus including cervix was removed vaginally. Histology confirmed uterine prolapse, a rare condition in bitches usually found shortly after birth especially