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Marker counter-selection via CRISPR/Cas9 co-targeting for efficient generation of genome edited avian cell lines and germ cells Anim. Biotechnol. (IF 1.487) Pub Date : 2021-03-02 Arjun Challagulla; Shunning Shi; Kiran Nair; Terri E. O’Neil; Kirsten R. Morris; Terry G. Wise; David M. Cahill; Mark L. Tizard; Timothy J. Doran; Kristie A. Jenkins
Abstract Efficient isolation of genetically modified cells that are phenotypically indistinguishable from the unmodified cells remains a major technical barrier for the broader utilization of CRISPR/Cas9. Here, we report a novel enrichment approach to select the genome engineered cells by co-targeting a genomically integrated GFP gene along with the endogenous gene of interest (GOI). Using this co-targeting
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Investigating genetic diversity and population phylogeny of five Chongqing local chicken populations autosomal using microsatellites Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-26 Xue Yang; Cheng-Li Liu; Bai-Gao Yang; Hai-Qiang Hu; Gong Ying; Guo Yi; Bo-Er Chen; Ying Yuan; He Yong-Meng; Wei-Yi Zhang; Dan-Ping Zhang; Guang-Xin E.; Ming-Hui Li
Abstract The genetic diversity and population structures of five Chongqing local chicken populations were investigated using by 24 microsatellite markers. Results revealed that the mean number of alleles (NA) ranged from 7.08 (Daninghe chicken, DN) to 8.46 (Nanchuan chicken, NC). The highest observed heterozygosity (HO) and expected heterozygosity (HE) were observed in DN (HO = 0.7252; HE = 0.7409)
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MiR-34c-5p promotes granulosa cells apoptosis by targeting Bcl2 in broody goose (Anser cygnoides) Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-25 Li’e Hou; Wangyang Ji; TianTian Gu; Kaiqi Weng; Di Liu; Yang Zhang; Yu Zhang; Qi Xu; Guohong Chen
Abstract Goose (Anser cygnoides) are highly broody with low egg production, and large number of granulosa cells undergo apoptosis during broodiness. Our previous study has found that miR-34c-5p was highly abundant in the ovary of goose with broodiness phenotype. However, the mechanism that miR-34c-5p regulates granulosa cells function remains unclear. Here, we demonstrated that broody goose had higher
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Expression and characterization of the serum proteome from yak induced into estrus by improved nutrition Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-24 Jianpeng Xie; Qudratullah Kalwar; Ping Yan; Xian Guo
Abstract Regulation of estrus plays a crucial role in the livestock industry. It is reported that providing better nutrition can induce early estrus in animals. However, little is known about the major endocrine and physiological mechanisms that could enhance estrus in anestrus animals. Hence in the current research two different groups of yaks, non-breeding season (February–June, NBS) estrus yaks
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Effect of replacing corn grains with date palm kernels on ruminal fermentation, feed degradability, and methane production under different initial in vitro pH conditions Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-23 Mahmoud Sabry; Mohamed E. A. Nasser; Hossam E. M. Kamel; Mohamed A. Abaza; Yosra A. Soltan
Abstract Date kernels (DK) are cheap by-products rich in energy and phenolic compounds. It can be used as an alternative to the conventional sources of energy in ruminant diets while reducing methane (CH4) production. Using a semi-automated gas production (GP) system, the initial pH of buffered rumen liquor was adjusted to 5.5 and 6.8. Five experimental diets were evaluated, control (0% DK), and DK25
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Evaluation of CD109, PCP4 and SEMA3D genes for their association with Ovine Johne’s disease in Turkish sheep Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-20 Yalçın Yaman; Ramazan Aymaz; Murat Keleş; Veysel Bay; Ecem Hatipoğlu; Cüneyt Kaptan; Atilla Başkurt; Onur Yilmaz; Michael P. Heaton
Abstract Johne’s disease is a chronic, contagious, zoonotic disease that affects numerous species including livestock and sometimes humans. The disease is globally distributed in sheep populations and caused by Mycobacterium avium Subsp. paratuberculosis (MAP). A previous genome-wide association study identified single nucleotide polymorphism (SNP) markers associated with OJD serostatus in CD109, PCP4
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Rich maternal and paternal genetic diversity and divergent lineage composition in wild yak (Bos mutus) Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-20 Zhi-Jie Ma; Li Guang-Zhen; Sheng-Mei Chen; Jian-Lin Han; Quratulain Hanif
Abstract Wild yak (Bos mutus) is a vulnerable bovine species on the Qinghai–Tibetan Plateau (QTP). So far, most studies on molecular genetic diversity of wild yak have focused on autosomal and mtDNA variations based on small number of samples. In this study, we analyzed 84 D-loop and 24 whole mitogenome sequences of wild yak to further comprehensively explore its maternal genetic diversity and lineage
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Prebiotics can restrict Salmonella populations in poultry: a review Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-19 Mohamed E. Abd El-Hack; Mohamed T. El-Saadony; Manal E. Shafi; Omniah A. Alshahrani; Sultan A. M. Saghir; Abdullah S. Al-wajeeh; Omar Y.A. Al-shargi; Ayman E. Taha; Nora M. Mesalam; Abdel-Moneim E. Abdel-Moneim
Abstract Antibiotics were over the years, the common supplement used for poultry production. There is a global trend to lessen antibiotics’ use due to the contamination of consumed meat with antibiotic residues. Also, there is a concern that human treatments might be jeopardized due to the emergence of antibiotic-resistant bacteria. Prebiotics are attractive supplements, particularly in poultry production
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Documentation of extensive genetic diversity in the Ovar-DRB1 gene in native Turkish sheep Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-19 Veysel Bay; Murat Keleş; Ramazan Aymaz; Ecem Hatipoğlu; Yasemin Öner; Yalçın Yaman
Abstract Indigenous breeds have a high level of genetic diversity that might contribute to develop animal breeds with desired traits such as disease resistance and high productivity. Major histocompatibility complex (MHC) is a key component of adaptive immune system and consists of highly polymorphic genes that take part in adaptive immune response and disease resistance. Exploring and understanding
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Identification of novel allelic patterns and evolutionary lineage of BoLA MHC class II DQA locus in indicine and taurine cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-18 Namita Kumari; S. K. Mishra; Shallu Saini; Anurag Kumar; Shubham Loat; Nitika Dhilor; S. K. Niranjan; Monika Sodhi; R. S. Kataria
Abstract Among different cattle types, Bos indicus are known for their ability to better resist the tropical microbial infections comparatively, wherein MHC molecules play a significant role. In this study allelic diversity at MHC locus, DQA of Bos indicus, Bos taurus and crossbred of taurine-indicus has been explored to understand the possible role of MHC region in differential immune response. Thirty
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Response of the ileum transcriptome to fructo-oligosaccharides in Taiping chickens Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-16 Fang Ma; Lintong Luo; Qianning Wang
Abstract The aim of this study was to investigate the effects of fructo-oligosaccharide (FOS) supplementation intake of Taiping chickens (Gallus gallus domesticus) and its stimulating effects on ileum. 120 healthy chickens were randomly divided into two groups; control group (CT) and fructo-oligosaccharides group (FOS). At the 60th day of age, ileum mucosa of three chickens per group were collected
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Determination of the relationship between Anatolian black cattle growth properties and myostatin, GHR and Pit-1 gene Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-15 Çağrı Melikşah Sakar; Uğur Zülkadir
Abstract The aim of this study is to determine the relationship between some growth and development characteristics in Anatolian Black cattle from birth to twelve months of age with the Pit-1, GHR and Myostatin genes. PCR-RFLP method was used to detect the polymorphism. Genotype and allele frequencies were found to be AA/AB/BB: 0.096/0.519/0.385 and A/B: 0.356/0.644; AA/AG/GG: 0.346/0.385/0.269 and
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A 67-bp variable duplication in the promoter region of the ADIPOQ is associated with milk traits in Xinjiang brown cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-15 Tingting Liu; Xing Ju; Menghua Zhang; Chen Wei; Dan Wang; Zhen Wang; Xianyong Lan; Xi-Xia Huang
Abstract Adiponectin, also known as ADIPOQ, is a hormone protein secreted by adipocytes. The ADIPOQ gene is expressed primarily in adipose tissue, and the encoded protein circulates in the bloodstream and has the potential to regulate both animal fat metabolism and hormone production. Our previous work uncovered a 67-bp variable duplication in the promoter region of ADIPOQ, which reduced the basal
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Genome-wide identification and functional prediction of circular RNAs in response to heat stress in Chinese Holstein cows Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-14 Ying Qi; Lin Zhang; Yuemei Guo; Jing Wang; Mingxing Chu; Yiming Zhang; Junfei Guo; Qiuling Li
Abstract Heat stress (HS) leads to substantial economic loss of dairy industry each year. The negative effect of HS in dairy cows is becoming one of the more urgent issue due to accelerating side-effects of global warming. Various genes are involved in HS response but the information about the role of noncoding RNAs, especially circular RNAs (circRNAs) is largely unknown. In our study, we aimed to
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Deletions in the KAP6-1 gene are associated with fiber traits in cashmere-producing goats Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-13 Jin Zhao; Qiang Ding; Lan Li; Peter Kalds; Shiwei Zhou; Jiayuan Sun; Shuhong Huang; Xiaolong Wang; Yulin Chen
Abstract Keratin-associated proteins (KAPs) are important structural components of fibers that predominantly present in the ortho-cortex. These proteins form a cross-linked network with keratin intermediate filaments (KIFs), thus producing a strong hair shaft. The keratin-associated protein 6-1 gene (KAP6-1) is a member of the KAPs family that has a potential correlation with fiber traits. In this
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Effects of dry yeast supplementation on growth performance, rumen fermentation characteristics, slaughter performance and microbial communities in beef cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-03 Siqiang Liu; Ali Mujtaba Shah; Mei Yuan; Kun Kang; Zhisheng Wang; Lizhi Wang; Bai Xue; Huawei Zou; Xiangfei Zhang; Peiqiang Yu; Hongze Wang; Gang Tian; Quanhui Peng
Abstract This study aimed to investigate the effects of active dry yeast (ADY) on growth performance, rumen microbial composition and carcass performance of beef cattle. Thirty-two finishing beef cattle (yak ♂ × cattle-yaks ♀), with an average body weight of 110 ± 12.85 kg, were randomly assigned to one of four treatments: the low plane of nutrition group (control), low plane of nutrition group + ADY
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Lemongrass supplementation to Farafra ewes improved feed utilization, lactational performance and milk nutritive value in the subtropics Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-02 Ahmed E. Kholif; Osama H. Matloup; Fatma I. Hadhoud; Ayman Y. Kassab; Moyosore J. Adegbeye; Hatem A. Hamdon
Abstract Application of phytogenic feed additives in livestock production is a sustainable practice and the search for more phytogenic options continues. This study was conducted to investigate the impact of lemongrass supplementation on nutrient utilization, rumen fermentation and milk production and composition. Thirty gestating Farafra ewes were randomly assigned to three experimental treatments
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Effects of dry yeast supplementation on growth performance, rumen fermentation characteristics, slaughter performance and microbial communities in beef cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-03 Siqiang Liu; Ali Mujtaba Shah; Mei Yuan; Kun Kang; Zhisheng Wang; Lizhi Wang; Bai Xue; Huawei Zou; Xiangfei Zhang; Peiqiang Yu; Hongze Wang; Gang Tian; Quanhui Peng
Abstract This study aimed to investigate the effects of active dry yeast (ADY) on growth performance, rumen microbial composition and carcass performance of beef cattle. Thirty-two finishing beef cattle (yak ♂ × cattle-yaks ♀), with an average body weight of 110 ± 12.85 kg, were randomly assigned to one of four treatments: the low plane of nutrition group (control), low plane of nutrition group + ADY
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Lemongrass supplementation to Farafra ewes improved feed utilization, lactational performance and milk nutritive value in the subtropics Anim. Biotechnol. (IF 1.487) Pub Date : 2021-02-02 Ahmed E. Kholif; Osama H. Matloup; Fatma I. Hadhoud; Ayman Y. Kassab; Moyosore J. Adegbeye; Hatem A. Hamdon
Abstract Application of phytogenic feed additives in livestock production is a sustainable practice and the search for more phytogenic options continues. This study was conducted to investigate the impact of lemongrass supplementation on nutrient utilization, rumen fermentation and milk production and composition. Thirty gestating Farafra ewes were randomly assigned to three experimental treatments
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Detection and whole genome sequencing of murine norovirus in animal facility in Italy Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-29 Silvia Tofani; Giovanni Ianiro; Luca De Sabato; Marina Monini; Giorgia Angeloni; Eleonora Ponterio; Claudia D’Agostino; Michele Angelo Di Bari; Mauro Valeri; Ilaria Di Bartolo
Abstract Viruses belonging to the genus Norovirus (NoV) of the family Caliciviridae are the major cause of acute viral gastroenteritis worldwide. NoVs are classified into 10 genogroups (GI-GX), and those belonging to the genogroup GV are able to infect several species of rodents. To evaluate the circulation of MNV among mice housed in an Italian facility, sampling was performed over two separate periods
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Effect of in ovo copper injection on body weight, immune response, blood biochemistry and carcass traits of broiler chicks at 35 days of age Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-29 Hanan A. Hassan; A. R. Arafat; K. Y. Farroh; M. S. Bahnas; I. El-wardany; Shaaban S. Elnesr
Abstract The current study was conducted to investigate effects of copper (sulfate, acetate and nano) in ovo injection at 10 days of the embryogenesis period on body weight (BW), immunity, biochemical parameters and carcass traits of broiler chicks at 35 days of age. A total number of 462 fertile eggs were used in seven groups, each group containing 66 eggs in three replicates. The experimental design
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Determination of γ-D-glutamyl-meso-diaminopimelic acid in rumen fluid of dairy cows by pre-column chiral derivatization-HPLC Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-24 Yan Wang; Qianyun Gao; Xiaoye Cheng; Guangjun Chang; Animesh Chandra Roy; Xiangzhen Shen
Abstract High concentrate (HC) diet feeding leads to the lysis of rumen microbes and the release of hazardous metabolites, which can trigger inflammatory responses, thereby impairing dairy cow health and production. γ-D-glutamyl-meso-diaminopimelic acid (iE-DAP), which constitutes the peptidoglycan (PGN) layer of bacteria, is the minimum PGN structure capable of activating inflammatory signaling pathways
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Establishment of primary chicken embryo myoblast cell culture, antigenic epitopes prediction and production of anti activin receptor type IIB polyclonal antibody in chicken Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-24 Pothanaboyina Guru Vishnu; T. K. Bhattacharya; Devara Divya; Athe Rajendra Prasad; N. Govardhan Sagar
Abstract The detection of activin receptor typeIIB (ACTRIIB) protein, a prominent negative muscle growth regulator has paramount value in augmenting growth traits through molecular breeding schemes in chicken. The study was formulated to establish primary chicken embryo myoblast culture (CEM) using 9th and 18th day chick embryos and to develop antibodies for immunodetection of ACTRIIB protein. The
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Nutritional significance and health benefits of omega-3, -6 and -9 fatty acids in animals Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-20 Mahmoud Alagawany; Shaaban S. Elnesr; Mayada R. Farag; Karim El-Sabrout; Othman Alqaisi; Mahmoud A. O. Dawood; Hidayatullah Soomro; Sameh A. Abdelnour
Abstract The balance between omega-3 (ω-3), omega-6 (ω-6) and omega-9 (ω-9) fatty acids (FAs) is very important because these types of oils constitute essential components for the formation of the cell membrane, also they are precursors for a large number of substances in the body. One of the most important strategies for improving the increment of polyunsaturated FAs in poultry and animal meat is
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Molecular characterization of porcine rotavirus A from India revealing zooanthroponotic transmission Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-18 Gazanfar Abass; Zunjar Baburao Dubal; Kaushal K. Rajak; Balasaheb M. Kale; Abhay Raorane; Nitin Dudhe; Bilal Ahmad Malla; Dhananjay Desai; Dharmendra K. Sinha; Obli R. Vinodh Kumar; Yashpal Singh Malik
Abstract Rotaviruses A (RVA) are leading causes of diarrhea and dehydration in piglets and imply great economic loss to the pig farming community. In this study, the porcine RVA genotypes circulating in western and northern parts of India were determined by screening 214 fecal samples from diarrheic (n = 144) and non-diarrheic (n = 70) pigs. Subsequently, the structural (VP4 and VP7) and nonstructural
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Temporal microRNA expression profile of pig peripheral blood during postnatal development Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-18 Zhijun Zhong; Xingxing Zhu; Qianzi Tang; Liang Hong; Yiren Gu; Zhiping He; Xuan Tao; Xuemei Yang; Yan Liang; Linyuan Shen; Ya Tan; Kai Zeng; Sancheng Ying; Yuekui Yang; Yunfeng Lei; Yan Wang; Jianjun Gong; Xiaohui Chen; Rui Zhou; Li Zhu; Xuebin Lv
Abstract Gene expression profiles of blood can reflect the physiopathologic status of the immune system. The dynamic microRNA (miRNA) expression profiles of peripheral blood from pigs at different developmental stages, and how differential expression of miRNAs might relate to immune system development, are unknown. In this study, peripheral blood samples taken at five developmental stages were used
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A 1.6 Mb region on SSC2 is associated with antibody response to classical swine fever vaccination in a mixed pig population Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-15 Arnav Mehrotra; Bharat Bhushan; Amit Kumar; Manjit Panigrahi; Karthikeyan A.; Akansha Singh; Ashok K. Tiwari; Hubert Pausch; Triveni Dutt; Bishnu P. Mishra
Abstract Classical Swine Fever (CSF) is a contagious viral disease of pigs which is endemic in several parts of the world, including India. Prophylactic vaccination using live attenuated vaccine is the preferred method of control. However, there is significant inter-individual variation in the antibody response to vaccination. In this study, we measured the E2 antibody blocking percentage after 21 days
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Evaluation of genetic diversity and population structure in four indigenous duck breeds in Vietnam Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-15 Lan Doan Pham; Duy Ngoc Do; Le Quang Nam; Nguyen Van Ba; Pham Hai Ninh; Doan Phuong Thuy; Pham Van Son; Pham Cong Thieu
Abstract This study characterized genetic diversity and population structure of four indigenous Vietnamese duck breeds and an exotic breed for setting the conservation priority. A total of 200 samples from four duck breeds (Sincheng, Minhhuong, Muongchieng and Bauben) and an exotic breed (Supermeat) were genotyped for fifteen microsatellite markers. The average number of alleles per locus was 14.07
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Complete mitogenome of Treron sphenurus (Aves, Columbiformes): the first representative from the genus Treron, genomic comparisons and phylogenetic analysis of Columbidae Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-13 Yun-Xia Chen; Cheng-He Sun; Yao-Kai Li; Yi-Ling Fei; Xiao-Ming Xue; Sen-Lin Hou; Yong-Wu Zhou; Jing Jiang; Hai-Tao Guo
Abstract The wedge-tailed green pigeon (Treron sphenurus) has a protective value in the evolution of the family Columbidae. In this study, the complete mitogenome of T. sphenurus from Baise City, China, which represents the first sequenced species of the genus Treron in Tribe Treronini, is reported. This was accomplished using PCR-based methods and a primer-walking sequencing strategy with genus-specific
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A variant associated to IGF-1 mRNA and protein expression in sheep Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-11 Luis A. Flores-Encinas; Felipe A. Rodríguez-Almeida; Monserrath Felix-Portillo; Francisco J. Jahuey-Martínez; José A. Martínez-Quintana
Abstract The insulin-like growth factor-1 (IGF-1), a key hormone in muscle development was investigated for single-nucleotide polymorphisms (SNPs) upstream of the IGF-1 gene and their effects upon its cognate mRNA and hormone levels in sheep. A 70 d feeding trial was conducted with 22 F1 (Dorper × Pelibuey) lambs, individually allocated and fed a diet with a forage-to-concentrate ratio of 36:64 and
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Identification of novel single-nucleotide polymorphism at exon1 and 2 region of B4GALT1 gene and its association with milk production traits in crossbred cattle of Kerala, India Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10 Jamuna Valsalan; Tina Sadan; Thirupathy Venkatachalapathy; Kulangara Anilkumar; Thazhathu Veettil Aravindakshan
Abstract Beta 1,4-galactosyltransferase-I gene (B4GALT1) is an important candidate gene for milk performance traits, encodes catalytic part of lactose synthesis. Main objectives of present study is identification of single-nucleotide polymorphism in exon 1 and 2 region of B4GALT1 and to find significant association of genetic variants with milk performance traits in crossbred cattle of Kerala. The
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AFLP markers based genetic diversity and population structure analysis of Kadaknath: an indigenous black meat poultry breed of India Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10 Shashi Bhushan Tripathi; Gayaprasad Jatav; Anoop Anand Malik; Shrikant Joshi; Vivek Kumar Singh; Madan Singh Negi; Laxmi Chauhan; Shyam Sundar Sharma
Abstract The current study is the first worldwide to assess the genetic diversity of Kadaknath poultry using amplified fragment length polymorphism (AFLP) markers. Out of total 96 accessions, four were outliers and 92 were Kadaknath accessions of which 30 were males, 62 were females. Of these, 74 were jet black, 7 penciled and 11 were golden feather color type of Kadaknath. High level of polymorphism
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Microcapillary LAMP for rapid and sensitive detection of pathogen in bovine semen Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10 Syed Atif Ali; Nongthombam Boby; Prasanna Preena; Shiv Varan Singh; Gurpreet Kaur; Subrata Kumar Ghosh; Sukdeb Nandi; Pallab Chaudhuri
Abstract A microcapillary-based loop-mediated isothermal amplification (µcLAMP) has been described for specific detection of infectious reproductive pathogens in semen samples of cattle without sophisticated instrumentation. Brucella abortus, Leptospira interrogans serovar Pomona and bovine herpesvirus 1 (BoHV-1) cultures were mixed in bovine semen samples. The µcLAMP assay is portable, user-friendly
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Correction Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-10
(2021). Correction. Animal Biotechnology. Ahead of Print.
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Influences of vitamin A, L-carnitine, and folic acid in ovo feeding on embryo and hatchling characteristics and general health status in ducks Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-07 Ahmed Gouda; Samar A. Tolba; Khalid M. Mahrose
Abstract The current investigation was conducted to test the potential effects of in ovo feeding of vitamin A, L-carnitine, and folic acid on embryonic growth and post-hatch performance. A total of 450 fertile duck eggs were randomly distributed into two experiments of five groups/experiment (255 eggs/experiment and 45 egg/group). The experimental groups were: negative control (non-injected eggs),
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Identification of three new microsatellites and their effects on body measurement traits in pigs using time of flight-mass spectrometry (TOF-MS) Anim. Biotechnol. (IF 1.487) Pub Date : 2021-01-05 Yanghai Zhang; Xuelian Zhang; Xingxing Xue; Wenjuan Shen; Lei Wang; Yuhong Ma; Jiping Zhou; Guofang Wu; Chuanying Pan
Abstract The body status of livestock affects their physiological function and productive performances. Microsatellites, one of the most used DNA markers, have been found to be associated with pig productive traits. However, their identifications and effects on body measurement traits of the Chinese Qinghai Bamei pig still uncovered. According to our previous sequencing data, in this study, three novel
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Strategies for transfection of bovine mesenchymal stem cells with pBC1-anti-CD3 vector Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-30 Fernanda Borges Duarte; Marcelo de Macedo Brígido; Eduardo de Oliveira Melo; Sônia Nair Báo; Carlos Frederico Martins
Abstract Cells from different origins behave differently regarding the incorporation of exogenous DNA and formation of transgenic cells. Milk production of recombinant antibody may benefit from efficient transfection protocols to produce transgenic animals. In this context, the objective of this study was to verify the transfection potential of bovine mesenchymal stem cells from Wharton’s jelly (MSC-WJ)
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Detecting fecal egg count (FEC) for gastrointestinal nematodes of adult Turkish sheep with different scrapie related PRNP haplotypes Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-23 Yalçın Yaman; Bayram Şenlik; Mehmet Özüiçli; Murat Keleş; Ramazan Aymaz; Veysel Bay; Ecem Hatipoğlu; Seyrani Koncagül; Yasemin Öner; Cemal Ün
Abstract Scrapie is a transmissible spongiform encephalopathy caused by prions and leads to neurodegeneration in the Central Nervous System (CNS) of sheep and goats. Genetic resistance/susceptibility to scrapie is well studied and it is known that the variations of 136th, 154th and 171st codons at the ovine PRNP gene have a major effect on the development of the disease. Many studies demonstrated that
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Characterization and phylogenetic evolution of mitochondrial genome in Tibetan chicken Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Xiaoxu Jia; Junxian Lu; Xiujun Tang; Yanfeng Fan; Qinglian Ge; Yushi Gao
Abstract The objective of this study was to characterize mitochondrial genome and investigate phylogenetic evolution in Tibetan chicken. In this study, four haplotypes were identified based on D-loop sequencing in Tibetan chicken (n = 40), and each representative of four haplotypes was selected for total mitochondrial genome sequencing and analyzed together with published mitochondrial genome data
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Identification of a promoter polymorphism affecting GPAT3 gene expression that is likely related to intramuscular fat content in pigs Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Cai Ma; Yi Sun; Jiying Wang; Li Kang; Yunliang Jiang
Abstract The intramuscular fat content (IMF) is an economically important trait in pigs and the Laiwu pig is famous for its excessively extremely high level of IMF. Our previous transcriptome study revealed that the dynamic expression of glycerol-phosphate acyltransferase 3 (GPAT3) is consistent with changes in the IMF of Laiwu pigs. In this study, we further analyzed the expression and polymorphism
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Seasonal pattern in occurrence of rotavirus infection (RV) in diarrheic children, calves and piglets from Bareilly, India Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Bilal Ahmad Malla; Zunjar Baburao Dubal; Anukampa Kadwalia; Gazanfar Abass; Obli Rajendran Vinodh Kumar; Ashok Kumar; Kaushal Kishore Rajak; Ishfaq Maqbool; Aquil Mohmad; Vivekanandhan Rangaraju; Arfa Fayaz
Abstract Rapid and reliable diagnosis for diarrhoeal disease is critically important for the differentiation of etiological agents and subsequent suitable treatment modalities. The objective of the study is to reveal the seasonal pattern in the occurrence of rotavirus in diarrheic children, calves and piglets from Bareilly, Uttar Pradesh, India. A total of 115 diarrhoeal samples were collected, out
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Whole-genome resequencing reveals aberrant autosomal SNPs affect chicken feathering rate Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-21 Mohan Qiu; Chaowu Yang; Huarui Du; Qingyun Li; Zengrong Zhang; Xia Xiong; Chunlin Yu; Xiaoyan Song; Chenming Hu; Bo Xia; Li Yang; Han Peng; Lan Liu; Xiaosong Jiang
Abstract Previous studies have shown that the feather growth rate of chicks is determined by two alleles located on the sex chromosome Z; however, in chicken production, feathering is usually not consistently controlled by the sex chromosome. To identify whether the feathering rate is related to autosomal inheritance, whole-genome resequencing was performed in eight chickens with slow- and fast-feathering
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Identification, biogenesis and function prediction of novel circRNA during the chicken ALV-J infection Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-16 Ting Yang; Lingling Qiu; Meng Bai; Laidi Wang; Xiaodan Hu; Lan Huang; Guohong Chen; Guobin Chang
Abstract Circular RNA (circRNA) is a new non-coding RNA with a highly conserved and stable covalently closed loop structure, and it plays an important role in a variety of biological processes and the occurrence of diseases. Based on the sequencing results, circRNA_3079 had the most significant difference between the infected group and normal group, up to about 8 times. It has attracted our attention
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Effects of different levels of dietary black cumin (Nigella sativa L.) and fenugreek (Trigonella foenum-graecum L.) and their combination on productive traits, selected blood constituents, microbiota and immunity of broilers Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-16 Vito Laudadio; Mohsen Nasiri-Dehbaneh; Rana Muhammad Bilal; Ali Qotbi; Faramin Javandel; Abbas Ebrahimi; Alireza Seidavi; Marina Slozhenkina; Ivan Gorlov; Peter G. Dunne; Vincenzo Tufarelli
Abstract The effects of inclusion of powdered seeds of black cumin (B) (Nigella sativa L.) and fenugreek (F) (Trigonella foenum-graecum L.) on productive traits, selected blood constituents, microbiota and immunity of broilers were studied. A total of 648 day-old chicks were randomly assigned to nine treatments, with four pen replicates, each with 18 birds, including three levels of B seed powder (BSP;
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A molecular marker of milk composition traits in NCAM2 gene of Chinese Holstein Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-14 Xiaoting Ding; Zijing Zhang; Ruifang Hu; Yifan Wen; Yongzhen Huang; Qiaoting Shi; Yajie Feng; Eryao Wang; Chuzhao Lei; Hua He
Abstract The aim of this study was to detect the novel copy number variation (CNV) locus of NCAM2 gene in Chinese Holstein, and to analyze the effect of the novel CNV locus in NCAM2 gene on milk composition traits. The novel CNV locus of NCAM2 gene in 310 Chinese Holstein was detected by real-time quantitative fluorescent PCR (qPCR) and association analysis was performed between the novel CNV locus
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Effects of sea buckthorn puree on risk factors of cardiovascular disease in hypercholesterolemia population: a double-blind, randomized, placebo-controlled trial Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-10 Fangfei Zhou; Jian Zhang; Ai Zhao; Yumei Zhang; Peiyu Wang
Abstract To investigate the effect of sea buckthorn puree consumption on reducing blood lipid and other risk factors of CVD. A total of 111 patients with hypercholesteromia who were treated with 90 ml sea buckthorn puree or placebo for 90 days were enrolled in this trial. Physical examination and analysis of lipid markers, hsCRP concentrations and cell adhesion protein concentrations with fasting blood
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Analysis of single nucleotide polymorphisms related to heifer fertility in Hanwoo (Korean cattle) Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-07 Seung-Hwan Lee; Dooho Lee; Mingyung Lee; Seung-Heui Ryoo; Seongwon Seo; Inchul Choi
Abstract Genome-wide association studies (GWAS) have accelerated the identification of functional trait loci in cattle and identified single nucleotide polymorphisms (SNPs) in candidate genes associated with fertility and production traits in high milk yield dairy cattle. The fertility of Hanwoo (Korean native beef cattle) has declined after the adaptation of a selection program for high quantity and
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Derivation and characterization of putative embryonic stem cells isolated from blastoderms of Taiwan Country chicken for the production of chimeric chickens Anim. Biotechnol. (IF 1.487) Pub Date : 2020-12-02 Chalothon Amporn; Pin-Chi Tang; Chien-Kai Wang
Abstract The conservation of Taiwan Country chicken (TCC) is important due to concerns for the local breed’s adaptability to the area and disease resistance. Furthermore, the genetic resource base of native chickens can be used to improve egg and meat production efficiency in commercial TCC. As the embryonic stem cells (ESCs) hold great potential for regenerative medicine and species conservation,
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Preparation and characterization of titanium dioxide nanoparticles and in vitro investigation of their cytotoxicity and antibacterial activity against Staphylococcus aureus and Escherichia coli Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-28 Salim Albukhaty; L. Al-Bayati; H. Al-Karagoly; S. Al-Musawi
Abstract Titanium dioxide nanoparticles (TiO2 NPs) are photo-active metallic nanoparticles becoming promising agents in modern biomedical applications. Herein, a novel process for the synthesis of TiO2 NPs with high stability was developed by a sol–gel process and to investigate their cytotoxicity and antibacterial activity. Numerous experiments have been performed to confirm the morphologies, compositions
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Fish tyrosinase enzyme involved in melanin biosynthesis: Insights from physicochemical characterization, homology modeling, and virtual screening studies Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-26 Riya Kumari; Shrinivas Jahageerdar; Archana N Panche; Sanath Kumar
Abstract In the vertebrates, including fish, the tyrosinase enzyme plays an essential role in coloration. Modulation of tyrosinase activity is expected to alter the body pigmentation in fish and other vertebrate species. In the present study, physicochemical, functional, and structural properties of tyrosinase of three fish species viz., goldfish, Japanese medaka, and common carp were determined. The
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Effect of indel variants within the sorting nexin 29 (SNX29) gene on growth traits of goats Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-19 Yi Bi; Yuhan Chen; Dongyun Xin; Tingting Liu; Libang He; Yuxin Kang; Chuanying Pan; Weijun Shen; Xianyong Lan; Mei Liu
Abstract The sorting nexin 29 gene (SNX29) is a well-known regulator of myocyte differentiation and proliferation. In this work, two indels (17-bp and 21-bp) were identified in the goat SNX29 gene, and their effects on the growth traits of 1,759 Shaanbei white cashmere (SBWC) goats were analyzed. Both indels had three genotypes [homozygote wild type (II), heterozygote (ID), and homozygote mutation
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Effect of dietary supplementation of wildCumin (Bunium persicum) seeds on performance, nutrient digestibility and circulating metabolites in broiler chicks during the finisher phase Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-11 Muhammad Shuaib; Nasr Ullah; Abdul Hafeez; Ibrahim A. Alhidary; Mutassim M. Abdelrahman; Rifat Ullah Khan
Abstract The objective of the present study was to evaluate the impact of inclusion of wild cumin seeds (WCS) on performance, nutrient digestibility and blood profile in broilers during the finisher phase. For this purpose, 360, 14 days old chicks were randomly divided into four groups designated as CONT (control), 0.5WCS, 1.0WCS and 1.5WCS with 5 replicates, which were supplemented with WCS at the
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Development of TaqMan PCR assay for detection of A and B variants of the bovine β-lactoglobulin Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-11 Svetlana N. Kovalchuk; Anna L. Arkhipova
Abstract β-Lactoglobulin (BLG) is one of the prevalent whey protein in cattle. To date, several variants of bovine BLG have been found, but the most common are A and B, which differ from each other by SNPs rs109625649 and rs110066229. Numerous studies showed effects of A and B variants of BLG on milk yield, fat and protein content and cheese-making properties. To date, polymerase chain reaction-restriction
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Expression and polymorphism of Follistatin (FST) gene and its association with growth traits in native and exotic chicken Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-10 K. Dushyanth; R. Shukla; R.N. Chatterjee; T. K. Bhattacharya
Abstract Follistatin (FST), a member of the transforming growth factor beta super-family regulates body growth by inhibiting the binding of myostatin (an inhibitor of growth) with its receptor in chicken. An experiment was conducted to explore ontogenic expression of the follistatin gene, determine polymorphism at the coding region of the gene and estimate its effect on growth traits in native (Aseel)
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Association between MYLK4 gene polymorphism and growth traits at different age stages in Anatolian black cattle. Anim. Biotechnol. (IF 1.487) Pub Date : 2020-09-24 İbrahim Aytekin,Mervan Bayraktar,Çağrı Melikşah Sakar,İlker Ünal
Abstract This study aims to find the association between MYLK4 gene polymorphism and growth traits at different ages, such as birth, 3rd-month, 6th-month and 12th-month ages in Anatolian black cattle. PCR-RFLP method was used to detect the polymorphism for MYLK4 gene in at G61595A locus. Allele and genotype frequencies were A: 0.34, G: 0.66 and AA: 0.17, AG: 0.34 and GG: 0.49. The chi-square χ2 test
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Daidzein stimulates fatty acid-induced fat deposition in C2C12 myoblast cells via the G protein-coupled receptor 30 pathway Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-08 Chengjian Zhou; Ping Li; Meihong Han; Xuejun Gao
Abstract Fat deposition in skeletal muscle is an important aspect of improving meat quality. Isoflavones can promote animal anabolism, but whether and how they regulate muscle fat deposition remain largely unclear. In this study, we explored the role and corresponding molecular mechanism of one of the major isoflavones, daidzein, in fat deposition in C2C12 myoblast cells. In the absence of fatty acids
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Oral administration of potato peel extract affects serum blood metabolites, liver function and ameliorating oxidative stress induced in rabbits exposed to cold stress Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-08 Yassmine M. El-Gindy; Salma H. Abo-Hafsa; Moyosore J. Adegbeye; Abdelfattah Z. M. Salem; Waleed M. Dosoky
Abstract This study investigated the effect of potato peel extract (PPE), orally administrated to rabbits, on serum blood metabolites and ameliorating oxidative stress induced by cold stress under Egyptian winter conditions. Twenty-four bucks grouped into three treatments (8 animals per group) were used for the experiment. The animals received 1.5 ml of water orally, containing 0 (PPE0), 25 (PPE25)
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A nonsynonymous SNP within the AMH gene is associated with litter size in Dazu black goats Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-05 Yan-Guo Han; Yan Zeng; Yong-Fu Huang; De-Li Huang; Peng Peng; Ri-Su Na
Abstract AMH, KISS1R and GDF9 genes play a vital role in human and animal reproduction and might be used as the genetic markers for the reproduction traits selection. The aim of this study was to screen the single nucleotide polymorphisms (SNPs) within the AMH, KISS1R and GDF9 genes and to determine the correlations between these SNPs and the litter size in goats. Nine single SNPs within these genes
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Transcriptional regulation of adipogenic marker genes for the improvement of intramuscular fat in Qinchuan beef cattle Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-05 Guo Hongfang; Rajwali Khan; Sayed Haidar Abbas Raza; Kaster Nurgulsim; Syed Muhammad Suhail; Abdur Rahman; Ijaz Ahmed; Asim Ijaz; Iftikhar Ahmad; Zan Linsen
Abstract The intramuscular fat content plays a crucial role in meat quality traits. Increasing the degree of adipogenesis in beef cattle leads to an increase in the content of intramuscular fat. Adipogenesis a complex biochemical process which is under firm genetic control. Over the last three decades, the Qinchuan beef cattle have been extensively studied for the improvement of meat production and
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Identification of important miRNAs in bubaline mammary gland at heifer stage - An in-silico approach Anim. Biotechnol. (IF 1.487) Pub Date : 2020-11-05 S. M. K. Thirumaran; Manjit Panigrahi; Sheikh Firdous Ahmad; Triveni Dutt; Bharat Bhushan
Abstract The present study was undertaken to identify the microRNAs (miRNAs) expressed in the mammary tissue of a bubaline heifer. Small RNAs were isolated from the mammary gland tissue and enriched for miRNA fraction. The linker-ligated small RNAs were reverse transcribed to synthesize cDNA and amplified by PCR. The PCR products were ligated to the pGEM-T Easy vector; cloned into DH5 alpha cells and
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