Pathogenic bacteria of Scophthalmus maximus enteritis were isolated from and identified in an aquaculture farm in Changli County, Hebei Province, China. The feasibility of rapid selection of prevention and treatment drugs via PCR technology was investigated through drug resistance phenotype analysis and drug resistance gene detection. A dominant bacterial strain was isolated from S. maximus and designated

Long-chain polyunsaturated fatty acids of n-3 family (n-3 LC PUFAs) are physiologically essential compounds required for normal growth and development of animals, including humans. The ability of fish species to synthesize n-3 LC PUFAs varies significantly across different trophic levels. We have studied fatty acid (FA) content (mg/g of wet weight) and level (% of total FAs) in the brain, liver, heart

Quantitative real-time RT-PCR (qRT-PCR) is the most sensitive and advanced method to quantify the expression of target genes of animals including fish. However, the broad variation in the expression patterns of housekeeping genes (HKGs) among tissues and different developmental stages makes it necessary to conduct studies for the selection of the suitable internal control. There is no report available

This study was dealt with induction of diploid meiotic and mitotic gynogenesis in Barbonymus gonionotus through cold shock treatment for production of monosex all-female population. For inhibition of paternal inheritance, sperm DNA was denatured by exposing cell suspension containing 8×108 ml-1 to UV at 196 μWcm-2 for 1.5 min. After fertilization of eggs using uv-treated sperm, meiotic diploidization

In recent years, the demand for commercial sea cucumber diet increased due to the expansion of sea cucumber farming. In this study, we investigated alternative sources other than the use of macroalgal powder for formulating sea cucumber feed. Specific growth rate (SGR), total weight gain, and daily weight gain were determined for sea cucumber (45 individuals, initial weight: 51.08±3.29g) fed with three

Goldfish is one of the preferred ornamental fish which is highly susceptible to cyprinid herpesvirus-2 (CyHV-2) infection. The present study aimed to analyse immune gene expression in a co-culture of CyHV-2-sensitized goldfish peripheral blood leukocytes (PBLs) with CyHV-2-infected fantail goldfish fin cell lines (FtGF). Goldfish were sensitized with intraperitoneal TCID50 dose (107.8±0.26/mL) of CyHV-2

Streptococcosis is a bacterial disease in tilapia that produces economic losses, caused mainly by Streptococcus agalactiae and S. iniae. It is treated using oxytetracycline and florfenicol, which when inappropriately used promotes the selection of antibiotic resistance genes (ARGs). The disease has been mainly associated with stress events such as variations in temperature. The aims of the present

A 12-week feeding trial was conducted to determine the optimal dietary selenium requirement for coho salmon (Oncorhynchus kisutch W.) alevins. Six experimental diets (contained about 42.2% crude protein and 10.5% crude lipid) were formulated to contain selenium level of 0.07, 0.15, 0.24, 0.41, 0.75, and 1.38 mg/kg, respectively. Each diet was fed to triplicate groups of fish with initial mean body

Grass carp (Ctenopharyngodon idella) is the most widely produced freshwater aquaculture product worldwide. However, its susceptibility to microbial infections during its cultivation restricts its development. The complement system plays an important role in immune defence. To investigate the transcription of the complement factor C5 and production of its cleavage product C5a in grass carp infected

The biofloc technology (BFT) is an intensive and sustainable production system, in which microorganisms can contribute to the maintenance of water quality and serve as a complementary food source for reared animals. The aim of this study was to evaluate three production strategies and whether the biofloc system enables the dietary reduction of crude protein (CP) levels for pacu (Piaractus mesopotamicus)

Stress-induced by low water-dissolved oxygen (DO) negatively influences the overall fish performances. Hybrid yellow catfish (Pelteobagrus fulvidraco × P. vachelli) was subjected to 2 DO levels: 1.5 mg/L (hypoxia) and 6.5 mg/L (control-normoxia) during the period of the experiment. Blood and liver were sampled at 0.0-, 6.0-, 12.0-, 24.0-, 48.0-, and 96.0-h post-exposure. Results showed an increase

Artemia franciscana metanauplii widely is being used in cultured marine species. The aim of this study was to evaluate the effect of feeding with two marine and two freshwater microalgae as feed alone or in combination on survival, growth, and fatty acid composition of A. franciscana metanauplii and on predominant bacterial species of the rearing water. Five microalgae diets were used for feeding:

This research aimed to elucidate the effect of Chlorella sp. addition on biofloc quality and growth of Macrobrachium rosenbergii juveniles in biofloc systems. Four treatments of freshwater prawn nursery culture systems were tested, i.e., control system with clear water system at a regular water exchange at about 50% every 2 weeks; control system with Chlorella sp. addition with regular water exchange

Nine semi-purified diets with three different levels of protein (350, 400, and 450 g/kg diet) and lipid (80, 120 and 160 g/kg diet) were fed ad libitum to Mystus gulio fry (0.16 g) in triplicate groups (20 fish/replicate) for 90 days. Flow-through fiber-reinforced tanks (50 L; water flow rate of 0.5 L/min) were used for rearing the fish. Result of the experiment showed that the dietary protein, lipid

This study aimed to evaluate the effect of dietary supplementation with cinnamon products on the growth, meat quality, liver structure, and blood composition of striped catfish. All diets were developed using intact protein and formulated to have similar protein (25.5–25.7%) and lipid (7.3–7.9%) contents. The control diet was formulated without cinnamon products (C), while dietary treatments were formulated

Stichopus cf. horrens is an emergent culture species. It is known to be nocturnal and negatively phototactic; hence, determining its behavioral and growth responses to different light regimes is essential in optimizing culture protocols. This study examined the interactive effects of shading and relative food availability on the feeding pattern, absolute growth rates, and survival of juvenile S. cf

An experiment was carried out to determine the effect of replacing dietary fish meal protein with earthworm meal protein on growth performance, whole-body composition, and hematology of butter catfish (Ompok pabda). This experiment was conducted in 15 glass aquaria (200 L) with five treatments, each having three replications. Each of the aquaria was stocked with 40 fishes (average weight 0.62 g). The

An integrated approach for rearing Pacific white leg shrimp (Litopenaeus vannamei) with red seaweed Agarophyton tenuistipitatum was evaluated in an experimental-scale in biofloc system. Accordingly, four treatments were designed and assessed the applicability of seaweed in biofloc-based intensive shrimp rearing system with respect to bioremediation and shrimp growth. Chemical oxygen demand, turbidity

Aquaculture is an important source of animal protein and a key contributor to global food security. However, aquaculture can exert a negative effect on the aquatic environment due to the release of effluents containing high nutrient levels. In integrated multi-trophic aquaculture (IMTA), the waste produced by one species is the input for another, referred to as extractive species (ES). Potential ES

Nanotechnology has become an important tool for fish disease prevention and treatment. The current study was carried out to evaluate the efficacy of dietary thyme essential oil (TEO) and its nano-emulsion (TEO-NE) for motile Aeromonas septicemia prevention (MAS) in Oreochromis niloticus after in vitro evaluation. Aeromonas hydrophila was isolated from naturally diseased Oreochromis niloticus and Clarias

The present study evaluated the effect of voluntary ingestion of duckweed (Lemna minor) on the growth and productive performance of Nile tilapia (Oreochromis niloticus) reared in a recirculation system (RAS). The constant availability of fresh lemna was compared with a control treatment without supplementation. In both treatments, the fish were fed a commercial feed for 28 days. The experimental design

The cultivation of Chlorella vulgaris in sludge extract (SE) from municipal excess sludge diluted with BG11 medium was investigated to achieve sludge reduction and microalgae cultivation. A maximum biomass of 1.404 ± 0.101 g/L was achieved at 15 days in a mixture of 25% BG11 and 75% sludge extract under 10,000-lx light. In early culture periods, heterotrophy dominated the process in the group with

The dietary effect of lemon essential oil loaded in chitosan nanoparticles (LEO/CNPs) on growth performance, serum biochemical parameters, humoral immune responses, and body composition in rainbow trout was evaluated. Two hundred and forty juvenile fish (4.74 ± 0.14 g) were randomly distributed to four experimental groups with three replicates in 12 fiberglass tanks (140 L; 20 fish per tank). The fish

Spirulina (Arthrospira platensis) is a microscopic filamentous cyanobacterium with a long history of basic and complementary alimentation. Nevertheless, a growing interest in its use in agriculture as biofertilizers and plant growth biostimulants was showed in the last years. Spirulina is commercially produced in large outdoor ponds under controlled conditions that affect both growth and biomass composition

Ammonia is the main environmental pollutant in aquaculture that has a negative impact on the healthy cultivation of common carp. The effects of antioxidant capacity, histopathology, and related gene expression of common carp (Cyprinus carpio) under ammonia stress (2.6 mM ammonia concentration, 168 h) were studied. In the study, ammonia stress can lead to oxidative stress in common carp, which is related

The study aimed to characterize the dynamics involved in the water quality parameters in a biofloc system (BFT) during the super-intensive cultivation of Litopenaeus vannamei using two levels of artificial brackish water. The test was designed with two salinity levels, T16 (16‰) and T8 (8‰), with 4 replicates, and 250 shrimps/m2 by 60 days, water analysis and animal performance were studied in each

Sperm handling procedures and its usage for in vitro fertilization are crucial for standardized experimental operations on evaluation of reproductive performance, gamete quality, and optimization of fertilization protocols. In our study, the changes in perch sperm motility parameters within 6-h chilled storage and using 4 different activating solutions were compared. Eight different sperm-to-egg ratio

Mycobacteriosis has been recognized as an infectious disease caused by Mycobacterium fortuitum in aquaculture. In Indonesia, mycobacteriosis outbreak has been detected in West Java, Central Java, and East Java. However, no studies have yet described the pathogenesis of mycobacteriosis in gourami. Therefore, this study was designed to detect the portal of entry and tissue distribution of M. fortuitum

Integrins, including the α and β subunits, are cell adhesion factors that promote intercellular and extracellular matrix (ECM) interactions. Integrin β1 (ITGβ1) is widely expressed in vivo. In addition, ITGβ1 has been shown to promote virus entry into the body. Currently, common carp is severely affected by herpesvirus in the aquaculture industry. However, ITGβ1 in common carp has not yet been identified

The irregular supply of natural mussel seed for aquaculture has seen an increase in the establishment of hatcheries to produce a reliable source of mussel seed. While many of the factors influencing fertilisation and early survival of hatchery-produced mussel larvae have been explored, little is known about the factors influencing the success of larvae during settlement and development under hatchery

For preventing transmission of pathogens from broodstock fish to offspring, the non-hardened eggs are routinely disinfected with iodophore solution immediately after fertilization. In the present study, 25–50 mg l-1 Chloramine T® (active substance: tosylchloramide), 10–50 μl l-1 Wofasteril® (active substances: acetylhydroperoxide, hydrogen peroxide, and acetic acid) and 10–50 μl l-1 of a 30% hydrogen

This study aimed to evaluate the effect of dietary supplementation of α-lipoic acid (LA) in the growth, blood chemistry, and liver antioxidant status of African catfish Clarias gariepinus. This study applied a completely randomised experimental design consisting of five experimental diets (30% protein and 4% lipid) with graded levels of LA, i.e. LA0.0 as the control (0.0 g LA kg−1 feed), LA0.5 (0.5g

Saprolegnia is one of the most potent fish pathogens and causes significant economic losses to the aquaculture industry annually. Thus far, almost all control methods have been ineffective or have caused environmental and fish-health-related problems. Vaccination of fish increases immunity against Saprolegniasis and provides an alternative to these control methods. We attempted to identify suitable

A 7-week feeding trial was conducted to investigate the effect of two protein hydrolysates: fish hydrolysate (FH) and shrimp hydrolysate (SH) as feed supplement on growth performance and feed utilization of juvenile striped catfish, Pangasius hypophthalmus. Three isonitrogenous (32% crude protein) and isolipidic (12% crude lipid) experimental diets were formulated: diet 1, control (no supplementation

The occurrence of fish diseases contributes to massive economic loss to the aquaculture industry. Edwardsiella tarda, a known pathogenic organism in Oreochromis mossambicus, infects several freshwater fishes. The present study attempts to find an effective treatment against E. tarda infection using natural plant-based bioproducts. Tamarindus indica seed extract was used to study its antibacterial activity

The growing intensive aquaculture system around the world maintains a high stocking density, wherein it is essential to increase and sustain the optimum dissolved oxygen concentration (DO) through the provision of artificial aeration systems. The selection of an aerator is a crucial aspect of aquaculture operations. The selected aerator must be economically efficient and should be able to fulfill the

Aquaponics is a food production system which connects recirculating aquaculture (fish) to hydroponics (plants) systems. Although aquaponics has the potential to improve soil conditions by reducing erosion and nutrient loss and has been shown to reduce food production related carbon emissions by up to 73%, few commercial aquaponics projects in the EU and UK have been successful. Key barriers to commercial

The development of rearing protocols promoting the larval development, pre and post-metamorphosis are key for echinoculture. Mixed diets combining diatom with other microalgae have been used with success and Rhodomonas sp. (Rho)-based diets showed promising results in larval survival. This study was aimed to evaluate the rearing success of Paracentrotus lividus larvae fed with mixed diets combining

A bioeconomic model and Monte Carlo simulation were used to analyze alternative management scenarios of partial harvesting schedules in semi-intensive shrimp (Litopenaeus vannamei) farming, emphasizing profitability, uncertainty, and economic risk. The schedules, including 1, 2, and 3 partial harvests, were studied considering two management scenarios (worst and best). Management improved by using

The world population continues to increase day by day. Therefore, aquaculture needs to grow in order to response the need for protein foods and to cope with the problem of scarcity. However, this enlargement should require taking place in a sustainable way in order to protect the environment and natural resources. The rapid development in the production of seafood as a food sector brought several issues

Estimation of realized fecundity (Freal, number of viable oocytes produced) is an essential, yet seldom-achieved element in the understanding of marine animal production and population dynamics. We used the Neutral Red (NR) vital stain to determine oocyte viability in spawns and gonad strippings of four species of commercially-important bivalves: Cerastoderma edule (L), Crassostrea gigas Thunberg,

Green mussel farming as a small-holder-friendly, livelihood-enabling supplementary activity along the backwaters in the southwest coast of India is analyzed in this paper covering various phases of its adoption and growth. Though a benign enterprise that does not demand significant establishment cost and labor-intensive culture activities, green mussel production is presently at a crossroads, facing

Sturgeons are an economically important freshwater aquacultural fish in China and elsewhere. Research was conducted to study the magnesium requirement of juvenile hybrid sturgeon (Acipenser schrenckii♀ × Acipenser baerii♂) based on mineral composition, proximate chemical analysis, antioxidant enzyme levels, and growth metrics. Different levels of magnesium supplements (43.2, 157.3, 326.5, 549.6, 743

Aeromonas hydrophila is one of the most important pathogens of zoonotic importance globally, causing various diseases in humans and animals. With the increase of drug resistance and the emergence of multidrug-resistant strains, therapeutic effects of antibiotics against A. hydrophila are limited. Hence, novel agents and strategies are urgently needed to overcome these difficulties. Wavy pili (W-pili)

Large-scale losses of spat are commonplace during the early stages of mussel aquaculture costing the industry millions in lost production. The dominant cause of this poor spat retention is thought to be secondary settlement behavior, whereby spat detach from farming substrata in an effort to relocate to another site. One approach that could be used to reduce these spat losses is to use natural substrates

Two mass mortality events (MMEs) of marine mussels that took place in the Oosterschelde, the Netherlands—the first in 2015/2016 and the second in 2019—both severely affected mussel production. The current study presents our observations on the onset and course of both MMEs and discusses probable putative causes. The two MMEs displayed a distinct course of events. The first event started in November

Heteropneustes fossilis is commonly known as singhi and considered an ideal fish species for aquaculture. It is much utilized for food and is known for medicinal properties in many parts of its range. In the present study, the influences of haematozoan infection, the fish individuals were found to be infected with the number of haematozoans of the group haemoflagellates and sporozoans of different

The fisheries and aquaculture sector plays an important role for food and nutrition security for the majority of people in Bangladesh. Recently, however, fish farmers and market actors face potential income loss due to post-harvest fish loss. This study assesses the factors influencing post-harvest loss of four important fish species (i.e., rui, catla, tilapia and pangas) in Bangladesh. Data were collected

The modulation of antioxidant responses and onset of oxidative stress is due to several factors, including temperature, diets, and hypoxia followed by re-oxygenation in several aerobic organisms including rotifers. In this context, the aims of this study were (1) to establish a protocol for evaluating in vivo ROS (reactive oxygen species) concentration in rotifer Brachionus plicatilis and (2) to determine

Molecular and immunological aspects of heterophyid infections in mullets are scanty. This study was initiated to identify heterophyid encysted metacercariae infecting gray mullet Mugil cephalus by morphological and molecular assays and to evaluate its immunological effects on infected mullets. Both farmed fish, from a private farm nearby Lake Manzala in Damietta, Egypt, and wild-caught fish, from the

Maintaining the balance of intestinal microbiota is vital for the growth and health of crustaceans. The current study explored the influences of the surface layer proteins (SLPs) of Pediococcus pentosaceus on the growth performance, disease resistance, and gut microbial community of the freshwater prawn Macrobrachium rosenbergii. After 28 days of feeding, obvious enhancements in the growth performance

The goals of this study were to evaluate growth, development, and calcification process of veligers of Strombus gigas grown in natural conditions in mesocosm versus laboratory conditions. In this study, larvae bred in mesocosm conditions had a good growth rate (33.3±12.40 μm.day−1) when fed with natural phytoplankton in natural flowing seawater versus a lower growth rate of 8.8±5.20 μm.day−1 for larvae

Misgurnus anguillicaudatus and Paramisgurnus dabryanus hybrids were generated via full diallel crosses between the Meishan (M_M) and Fuyang (M_F) populations of M. anguillicaudatus and Meishan (P_M) and Ziyang (P_Z) populations of P. dabryanus. The purebred and hybrid offspring were co-cultured in a pool for 4 months, and 96.5% of the offspring could be assigned to a single parental pair based on nine

This study was to evaluate the growth, intestinal morphology, intestinal flora, and anti-infection activity against Aeromonas hydrophila in common carp upon adding Bacillus licheniformis to the feed. Four experimental diets supplemented with different concentrations of B. licheniformis were as follows: 0 (CK), 106 colony forming units (CFU)/g (A), 107 CFU/g (B), and 108 CFU/g (C). After 60 days of

Several strains of pearl oysters (Pinctada fucata) are distributed in the coastal areas of South China. To explore strategies for genetic improvement, additive genetic, heterotic, and strain reciprocal effects on growth traits were estimated by performing a full 3 × 3 diallel cross of three pearl oyster strains that are local to Beihai, Guangxi Province; Xuwen, Guangdong Province; and Sanya, Hainan

Matrix metalloproteinase (MMP)-9 belongs to the zinc-dependent endopeptidase family, which is associated with important inflammatory processes and plays an important role in cell metabolism, embryonic development, and wound repair. The present study was performed using Pampus argenteus individuals fed with different feeds. The full-length cDNA of the MMP-9 gene was 2783 bp and encoded 681 amino acids

Food incidents have led to the increase in demand for food products certified by an international quality assurance certification of consumers all over the world. Shrimp is an important daily food product. As one of the largest shrimp production countries with the export-orientation, Vietnamese shrimp producers have to consider applying for the international quality assurances certifications to be

Edwardsiella piscicida (E. piscicida) is a common pathogen in freshwater and marine environments and an important intestinal pathogen of fish. Fucose large presence in the intestines could possibly regulate relevant genes to promote bacterial infectivity. In this study, the wild-type E. piscicida and the fucP gene-deleted strain ΔfucP were used to identify its virulence-regulating pathway and colonization