A feeding trial was conducted to evaluate the effects of the fishmeal level in the diet of the oriental river prawn, Macrobrachium nipponense, on growth performance and gene expression related to protein and lipid metabolism. Six isonitrogenous (41%) and isolipid (8.5%) diets were designed with decreasing fishmeal (FM) levels from 35% to 30%, 25%, 20%, 15%, and 10%. Each diet was fed to three tanks

The worldwide decrease in fishery stocks in recent years is related to the degradation of coastal environments and to the increases in capture effort and extreme natural events. Restocking dilapidated populations with artificial hatchery-reared larvae has arisen in recent years as a viable technique for the reparation and maintenance of stocks of commercially exploited species. One of the first steps

This study evaluated the hematological, biochemical, and histopathological responses of sorubim Pseudoplatystoma spp., experimentally infected with Lactococcus garvieae. Sorubim juveniles (n = 66; 21.3 ± 5.5 g; 17.1 ± 1.3 cm) were infected with L. garvieae by coelmatic inoculation (1.5 × 108 CFU mL−1) and sampling to 1, 3, 7, 14, and 21 days post-inoculation (dpi) for evaluation of hematological, biochemical

Myxozoans can induce losses in production of marine and freshwater fish. Recently, a new parasitic myxozoan species, Sinuolinea niloticus, was identified and its importance highlighted. This study aimed to investigate Sinuolinea niloticus and its macroscopic and microscopic alterations in Nile tilapia (Oreochromis niloticus) specimens from fish farms in Botucatu, São Paulo, Brazil, as well as its molecular

Black carp (Mylopharyngodon piceus) is one of traditionally important aquaculture fishes in China; however, population genetic studies are relatively rare for this species. In the present study, we developed a set of microsatellite markers by FIASCO method and used ten of these polymorphic markers to evaluate genetic diversity of black carp (three wild populations and six cultured populations including

More and more studies have revealed a close relationship between gut microbial community and human health. However, the mechanism why changes in gut microflora structures affect shrimp health status remains unclear. In this study, we compared the differences of bacterial communities among intestine, water, and sediment collected from mariculture ponds of Penaeus japonicus in the early (30 days) and

The present study evaluated the effect of probiotic Lactobacillus sp. against pathogenic bacteria Vibrio harveyi in freshwater prawn Macrobrachium rosenbergii. The bacteria were isolated from the gut of prawn and identified using biochemical and PCR tests. Inoculation of probiotic (9 log CFU/g) and pathogenic bacteria (5 log CFU/g) mixture was employed for in vitro test. The in vivo challenge test

Aquaculture and capture production of clam species have great economic value in Europe. The production is dominated by the introduced species, Manila clam, Ruditapes philippinarum, which has displaced some of the native species of clams. In Europe, landings of autochthonous clams have decreased in the recent years due to overfishing, failure in recruitment, abiotic stress and diseases. Due to aforementioned

Tolerance for acute ammonia exposure, defined to exist between NOEC (no observed effect concentration) and LOEC (lowest observed effect concentration), was validated in seven exposure experiments with Atlantic cod larvae from 15 to 63 days post-hatch, corresponding to larval sizes of 0.2 to 4.9 mg dry weight, respectively. LOEC was found in the range of 0.019–0.082 mg L−1 NH3-N, with corresponding

White spot syndrome virus (WSSV) is the most contagious pathogen causing huge economic losses in the shrimp industry worldwide. Several medicinal plants are known to have antiviral activity through the inhibition of viral diseases in fish and shellfish. Hence, there is a need to investigate the ability of natural remedies like plant sources to mitigate this devastating disease in crustaceans. This

This study was conducted to determine the localisation of antigen in the gut of vaccinated and non-vaccinated red hybrid tilapia post-challenge via immunohistochemistry and scanning electron microscopy. All groups (1, 2, 3, and 4) were vaccinated orally with feed-based killed whole-cells of Streptococcus iniae (FKV). Group 1 was fed with the FKV for 3 days consecutively only on day 0; group 2 was fed

Blood parameters in fish provide a rich source of information about metabolites, blood cells and biochemical markers that reflect individual differences in health, disease, diet and habitat. Haematological and biochemical parameters were investigated in 80 cultured striped bass Morone saxatilis, in order to evaluate a possible correlation between biometric indices (weight and total length) and blood

Spermatophore successive manual extrusions are often employed in hatcheries, but it causes melanin deposition, decreasing sperm quality via cell degeneration. Melanization (brown or black spots) is an indicator of the moment to discard males. The evaluation of more efficient strategies is required to avoid it, increasing males’ lifespan. The effects of two temperatures (26 and 30 °C) on closed thelycum

Organic aquaculture, including carp pond farming, is of growing importance. One of its main characteristics is the health and quality of water environments and maintenance of their biodiversity. In 2013–2014, four organic carp ponds (ORG) in the Waldviertel region (Austria) and four conventional ponds (CONV) in the Třeboň region (Czech Republic), located in the Lainsitz (Lužnice) river basin, were

The lecithin (LST) requirements for young and adult crustaceans have been investigated, and little information is available for early life stages. Therefore, our study aimed to determine the best Artemia enrichment levels with soy lecithin as a means of maintaining better survival, growth rate, and total length during the early stages (mysis 1 to post-larva 7) of the green tiger shrimp, Penaeus semisulcatus

Bacterial adhesion is considered as an important property that enables probiotic bacteria to colonise the gastrointestinal tract and confer a health benefit to the host. In the present study, a shrimp gut isolate Bacillus subtilis subsp. inaquosorum V1, an efficient producer of bacteriocin was used. Autoaggregation, co-aggregation and cell surface hydrophobicity assays were performed to confirm the

Shrimp is the most valued fish traded internationally, the USA, EU, and Japan being the major countries importing shrimp from Asian countries and Ecuador. Import rejections due to quality issues lead to substantial economic loss. Year-on-year change fails to capture the nature of rejection. Unit rejection rate (URR), relative rejection rate (RRR), the trend in shrimp exports, and causes of rejection

Aquaculture farms, such as the ones that produce mollusks, marine fish, and shrimp, require a constant supply of microalgae biomass as feed. Microalgae cultures are subject to failures in continuity; therefore, concentrated inocula could be used during episodes of crashes to reestablish the production of microalgae biomass. For this reason, the present work evaluated a concentrated inocula of the microalgae

This study presents the first report of myonecrosis and hepatopancreatic necrosis in broodstock of Litopenaeus vannamei. Of the 400 individuals examined, 34 displayed myonecrosis and hepatopancreatic necrosis, conditions previously reported to be caused by bacterial, fungal, parasite, and viral infections. Gross signs of infection include passive swimming, anorexic and the abdominal muscle become opaque

The increase of ammonia nitrogen, nitrite, nitrate, urea, and other dissolved nitrogen in prawn pond often causes eutrophication of water body and prawn poisoning, which brings great harm to aquaculture. Microalgae are the main biological factors in prawn pond, which can effectively utilize dissolved nitrogen. The goal of this study was to evaluate the effects of the forms and concentrations of various

Functional foods have molecules that promote health benefits beyond their nutritional qualities. In this context, the zootechnical performance, color, and biochemical responses of Pacific white shrimp (Litopenaeus vannamei) fed with different levels of açaí (Euterpe oleracea), a fruit with unique antioxidant characteristics, were observed. Four diets with different levels of inclusion of lyophilized

This study aims to evaluate the production performance of biofloc-based co-culture systems of Nile tilapia and redclaw crayfish with varying carbon–nitrogen ratios (C/N). The experiment was conducted for 80 days in plastic-lined ponds with dimensions of 1 x 1 x 0.6 m. Two biofloc systems with two C/N ratios (10 and 15), using molasses as the carbon source and a control system without molasses were

The hepatopancreatic disease in shrimp has caused great economic losses in recent years, and it is necessary to identify the responsible pathogen and establish control methods. The present study aimed to identify the primary pathogenic bacteria in diseased Litopenaeus vannamei hepatopancreas (HP) and to explore the protective effects of functional bacteria on shrimp. Four pathogenic Vibrio spp. were

The original article unfortunately contains interchanged first and family name of the authors.

The original version of this article unfortunately contains incorrect equation under the material and method section, in chitosan yield methods part of the formula.

While health benefits imparted by probiotics and prebiotics in various finfish have been extensively researched over the past few decades, little emphasis has been given to its role in immunomodulation in grouper fish health. From this billion-dollar aquaculture industry, the production of an estimated 100,000 tons per annum of grouper fish is required to fulfill the needs of the global market by 2020

In this study, we investigated the export performance and competitiveness of Chinese and Indonesian tilapia in the US market. We analyzed the factors that influence exports by using the constant market share model according to data from the USDA aquaculture database between 1996 and 2017. The results showed that the changes in the US market demand had a great impact on the tilapia industry in China

This study was conducted to investigate the effects of diet 7.5 g/kg α-ketoglutarate (AKG) on the growth performance, antioxidant defense system, digestive enzymes, and immune response of grass carp (Ctenopharyngodon idellus). A total of 400 grass carp with an average body weight 10.81 ± 0.68 g was randomly allocated into 2 groups with 4 replicates of 50 fish respectively. The experiment was conducted

Stock enhancement is a fishery management approach involving the release of hatchery organisms to increase the abundance of natural fish or invertebrate population. In this study, genetic evaluation of Fenneropenaeus chinensis stock enhancement was carried out based on the mitochondrial DNA control region. The haplotype diversity (Hd) and nucleotide diversity (π) of broodstock populations were generally

In the present work, fractal geometry is applied to histological sections of the hepatopancreas tissue of white shrimp (L. vannamei) experimentally infected with Hepatobacter penaei bacteria (NHPB), in order to perform an analysis of the morphology adopted by the different cellular components damaged by this intracellular-type pathogen bacteria. The fractal dimension of the digital images of the histological

One of the major problems in artificial eel reproduction is identification of broodstock with high performance reproductive characteristics. The aim of this study was to study population characteristics and the reproductive potential of eels (Anguilla anguilla) located in Valle Campo valley (Comacchio, Italy). A total of 200 eels/period (spring–summer and autumn–winter) were measured to obtain an external

The study evaluates optimum hydraulic loading rate (HLR) based on the fish and plant production performance and nutrient removal percentage in aquaponics. The growth of Gotukola (Centella asiatica (L.))—a leafy vegetable as well as traditional medicinal plant—and koi carp (Cyprinus carpio var. koi.)—a high demand ornamental fish—were evaluated at three different HLRs, viz. 2.6 m day−1 (T1), 7.8 m day−1

The chelating agent ethylenediaminetetraacetic acid (EDTA) is used throughout the world to improve the yield of early stage D-larvae during bivalve hatchery production. Adding EDTA (12 μM) to seawater significantly increases the survival of Greenshell™ mussel (Perna canaliculus) larvae during their first 48 h of development. However, whether there are benefits of continuing to use EDTA beyond this

The current study aimed to investigate the most prevalent ectoparasites among some Egyptian tilapia hatcheries and find out the appropriate therapeutic and prophylactic doses. For that, 500 Nile tilapia fry weighing 0.02 g were collected from several hatcheries at Sharkia Governorate, Egypt, over the period of April to September 2017, examined for the presence of trichodinids and monogeneans. The prevalence

Pikeperch (Sander lucioperca L.) is a freshwater fish species of major economic importance in several producer countries. Insufficient information about growth-related genes in pikeperch prevents their breeding through marker-assisted selection (MAS). This study aimed to clone growth-related genes (IGF-I, IGF-II, and GHR) of pikeperch to detect single-nucleotide polymorphisms (SNPs) associated with

This study aimed to investigate the effect of black cumin seed oil (BCSO) on the growth metrics, innate immunity and resistance against Pseudomonas fluorescens infection in Nile tilapia Oreochromis niloticus. This was done by conducting a feeding trial experiment on the fish. A total of four diets (protein, 35.99 ± 0.16%; lipid, 9.86 ± 0.06%) varying in their BCSO ration (w/w; 0% (control), 1%, 2%

This study aimed to evaluate the structural changes in the microbiota of Penaeus monodon during its ontogenetic development and evaluate functional shifts in metabolic pathways. To address these questions, shrimp were maintained under isolated conditions to avoid cross-contamination with other environmental bacteria and reared in twelve 50 L plastic tanks connected to a recirculation system. The replicates

Microalgae biomass is considered one of the most promising raw materials for the development of high commercial value products, mainly because they are potential sources of proteins, lipids, carbohydrates, and vitamins. In this way, the optimization of culture medium of these microorganisms can help to reduce production costs, making final products more accessible. In this sense, the present study

A feeding trial was conducted to investigate the effects of different levels (0%, 16.67%, 33.33%, 50%, and 66.67%) of fish meal replacement by protein mixtures on the growth, body composition, and physiological metabolism of juvenile swimming crabs, Portunus trituberculatus. The results showed that the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), intermolt duration (ID)

To limit the use of antibiotics in farmed fish and their potential negative impacts on public health and the environment, an evaluation of “functional alternatives” is required. The availability of effective treatments to control fish diseases is one of the most significant challenges facing aquacultures and veterinarians to reduce consequences of antimicrobial resistance. This paper includes results

Botryococcus braunii, a green alga that can produce a variety of secondary metabolites such as lipids, protein, and carotenoid, is considered a potential biodiesel feedstock for industrial commercialization. This study investigated the effects of exogenous plant growth regulators (PGRs) (6-benzylaminopurine (6-BA), abscisic acid (ABA), 2,4-epibrassinolide (EBR), ethephon (ETH), gibberellic acid (GA3)

The effect of stocking density and water exchange was evaluated on growth performance of Litopenaeus vannamei postlarvae (PL), reared in a biofloc-based system at the nursery level and exposed to short-term low-high salinity stress. A bioassay was conducted with four treatments in triplicate: T1 = 8500 postlarvae/m3 without water exchange, T2 = 16,500 postlarvae/m3 without water exchange, T3 = 8500

Saccharomyces cerevisiae biomass generated as a by-product in industrial processes and other biomasses grown in industry waste waters like Spirulina platensis and Rubrivivax gelatinous can be used in aquafeed, thus reducing the costs of discard and minimizing environmental damage. In this study, 840 male Nile tilapias (Oreochromis niloticus; 26.8 ± 1.03 g average weight) were distributed among 21 tanks

A lipase gene was cloned from Trichosporon fermentans Y3, subcloned into pPICZαA, and then expressed in Pichia pastoris X33. The lipase (lipRT), which was obtained using a 50-L bioreactor, had maximum activity of 6000 U/mL. It had an optimum pH 8.0 and remained stable between pH 3.0 and 11.0, while its optimum temperature was 45 °C, maintaining more than 55% of optimum activity between 20 and 35 °C

Social network analysis (SNA) is an efficient method to describe network characteristics from various specific aspects. In this study, it took world countries (areas) as the primary units of analysis and introduced different procedures of SNA, including network density, centrality, and core-periphery structure, and applied them to the field of international aquatic product trade (IAPT). Besides statistical

To utilize microalgae as a valuable bioresource, the effect of growth conditions on their yield and biochemical product production must be determined. We employed response surface methodology in the function of a Box-Behnken design to derive the functional relationship between environmental conditions and the microalgal biomass and lipid content of Chlorella pyrenoidosa. C. pyrenoidosa was grown in

Developing countries are far more vulnerable to climate change impacts than industrialised countries. Most of the world’s poor live in South Asia where they have limited livelihood options that have become even narrower in recent years, indicating a need for alternative income-generating options. Mud crabs (Scylla spp.) are considered to have promising prospects in different parts of the world including

Megalocytivirus of family Iridoviridae has been identified as a pathogen that caused the fatal systemic infection to lead to massive death of numerous fish species in both ornamental and food fish industries. It caused significant economic losses due to severe mortality of infected fish. Since 2011 in Indonesia, megalocytivirus in giant gourami (Osphronemus goramy) had been found as the main pathogen

The present study investigated the therapeutic effects of turmeric on the health of rohu (Labeo rohita) challenged with pathogenic Aeromonas veronii. The fish were fed on diets supplemented with different levels of turmeric for 120 days and then challenged with pathogenic A. veronii for 2 weeks, during which fish continued to receive respective turmeric-supplemented diets. Following trial, serum immune

The dietary calcium requirement of fingerling Labeo rohita (6.0 ± 0.11 cm; 1.94 ± 0.07 g) was examined using seven iso-nitrogenous and iso-caloric purified diets (350 g kg−1 crude protein; 16.72 kJ g−1 gross energy) with incremental levels of calcium (2.14, 3.12, 4.23, 5.11, 6.14, 8.23, and 11.24 g kg−1) that were hand-fed to triplicate groups of fish (20 fish per tank) to apparent satiation for 8 weeks

The transition from live to artificial feed is a critical step for fish larvae with potential for cultivation. Determining the moment to initiate the introduction of artificial feed based on larvae swimming behaviour can reduce mortality levels resulting from this dietary transition. Kinovea® is a cost-free software used as a tool to study sports kinematics; its use can help to establish locomotor

The incidence of infections by pathogenic microorganisms, coupled with the experimental manifestation of infections, refers to a regular phenomenon in aquaculture species. A number of environmental changes generally give rise to traumatic scenarios, and activating the transient provocative reactions in the gut normally constitutes a factor to the anatomical and efficient intestinal diseases. These

Dissolved oxygen (DO) is a vital nutrient for the health of water bodies such as streams, rivers, and ponds, as it is used in respiration and other biological activity of aquatic animals. Natural replenishment of oxygen through photosynthesis in aquatic bodies is insufficient to meet the high requirements for an intensive aquaculture system, which compels farmers to use aeration systems to supplement

Mytilus galloprovincialis is one of recent bivalve species produced in Portugal offshore. The growth, condition index, and the fatty acid composition of the mussel produced at 2 and 13 m depth was compared in longline on the south coast of Portugal. For each depth, a monitoring of several water quality parameters such as temperature, turbidity, nutrients, and chlorophyll-a was carried out. There were

In ornamental aquaculture, the traits related with beauty are an important factor in the economy and their inclusion in the genetic improvement program is essential. However, in some species, it is difficult to define which traits are important in this sense. The objective of this study was to know the producers’, traders’, and aquarists’ interests of morphometric characteristics associated with the

With the intensification and industrialization of aquaculture, there is an urgent need for new high-performance aeration devices and energy-saving aeration technology to achieve oxygen saturation. Based on the oxygen transfer equation between air and water two-phase and the Venturi principle, a new tube aeration device was designed, developed, and tested in an attempt to solve these problems. The structural

Tilapias are the most farmed tropical fish species in the world, and production globally was about 4.5 million tons in 2013 and is predicted to exceed 6.6 million tons by 2030. The emergence of diseases has led to significant loss to tilapia aquaculture over the years. Medicinal plants have been known for use in treating some human diseases and studied in aquaculture to find safe and eco-friendly compounds

Functional foods employ ingredients whose purpose is not necessarily to supply a possible nutritional deficiency, but rather to offer benefits to human and animal health. In this context, the present study aimed to investigate the use of oregano essential oil (OEO) and ethanolic extract of propolis (EEP) as functional foods for Nile tilapia challenged with Aeromonas hydrophila. Healthy fingerlings

Hematological studies of fishes are useful in the diagnosis of many diseases as well as investigation of the extent of damage to the blood. These parameters act as efficient and insightful index to examine health status, physiological and pathological changes occurring in various fishes, i.e., metabolic perturbations in fish body thereby acting as non-specific biomarkers in the field of environmental

Mycoplasma is associated with fatal diseases in human, animals, and fish. Mycoplasma of aquatic origin has not been thoroughly studied. The current study has shed the light on isolation and characterization of unique piscine Mycoplasma isolates retrieved from diverse geographical locations across the Egyptian state. Mycoplasma spp. was isolated using specific culture media. Mycoplasma was identified