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Prevalence of Borrelia burgdorferi and Anaplasma phagocytophilum in Ixodes ricinus collected from dogs in eastern Poland J. Vet. Res. (IF 1.8) Pub Date : 2024-03-11 Anna Pańczuk, Małgorzata Tokarska-Rodak, Patrycja Andrzejuk
Introduction Ixodes ricinus ticks are an important vector and reservoir of pathogenic microorganisms causing dangerous infectious diseases in humans and animals. The presence of ticks in urban greenery is a particularly important public health concern due to the potential for humans and companion animals to be exposed to tick-borne diseases there. The study assessed the prevalence of Borrelia burgdorferi
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Tissue expression of porcine transient receptor potential mucolipin protein channels and their differential responses to porcine reproductive and respiratory syndrome virus infection in vitro J. Vet. Res. (IF 1.8) Pub Date : 2024-03-11 Zhiqiang Xia, Denggao Long, Xinyi Hong, Ying Lan, Lixia Xie
Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) infection results in a serious disease, posing a huge economic threat to the global swine industry. The transient receptor potential mucolipin proteins (TRPMLs) have been shown to be strongly associated with virus infection and other physiological processes in humans, but their tissue distribution and responses to PRRSV in pigs
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Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus J. Vet. Res. (IF 1.8) Pub Date : 2024-03-05 Maria Szczotka, Jacek Kuźmak
Introduction Bovine leukaemia virus (BLV) is a Deltaretrovirus responsible for enzootic bovine leukosis, the most common neoplastic disease of cattle. It deregulates the immune system, favouring secondary infections and changes in the blood and lymphatic tissues. Blood homeostasis depends on functional haematopoietic stem cells (HSCs). Bone marrow is populated by these cells, which express CD34+ and
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Molecular survey and genetic diversity of Plasmodium sp. infesting domestic poultry in northeastern Thailand J. Vet. Res. (IF 1.8) Pub Date : 2024-02-28 Wasupon Chatan, Khanchit Khemthong, Kittiya Akkharaphichet, Punwara Suwarach, Tossapol Seerintra, Supawadee Piratae
Introduction Haemosporidian parasites are prevalent worldwide and can cause economic losses in poultry production. These parasites are arousing interest in Thailand and are found in many avian species. There is insufficient information on the genetic diversity of these alveolates from the largest families – Plasmodidae, Haemoprotidae and Leucocytozoidae – specifically parasitising ducks, turkeys, and
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Simultaneous determination of sulfonamides, trimethoprim, amoxicillin and tylosin in medicated feed by high performance liquid chromatography with mass spectrometry J. Vet. Res. (IF 1.8) Pub Date : 2024-02-26 Ewelina Patyra, Krzysztof Kwiatek
Introduction The article presents a rapid and simple analytical procedure for determination of four sulfonamides (sulfadiazine, sulfamerazine, sulfamethazine and sulfamethoxazole), trimethoprim, tylosin and amoxicillin in animal medicated feed. Material and Methods Eighteen medicated feed samples were analysed for active substances. The analytical protocol used a mixture of acetonitrile and 0.05 M
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Probiotic supplementation as an alternative to antibiotics in broiler chickens J. Vet. Res. (IF 1.8) Pub Date : 2024-02-26 Grzegorz Tomczyk, Jowita Samanta Niczyporuk, Wojciech Kozdruń, Anna Sawicka-Durkalec, Łukasz Bocian, Marcin Barabasz, Marcin Michalski
Introduction The broiler chicken digestive tract microbiome maintains the bird’s immunity. Its composition has been shown to be important not only for the immune system but also for the gastrointestinal function and productivity of broiler chickens. If the microbiome is populated by supplementation with Lactobacillus, Pediococcus and Saccharomyces spp. – microorganisms with probiotic properties and
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The first complete genome sequence and genetic evolution analysis of bovine norovirus in Xinjiang, China J. Vet. Res. (IF 1.8) Pub Date : 2024-02-19 Zhigang Ma, Qian Jiang, Chenxi Quan, Lu Liu, Zhonghua Zhang, Jinxing Xie, Lu Zhao, Qi Zhong, Gang Yao, Xuelian Ma
Introduction Viruses are among the main pathogens causing diarrhoea in calves. The current study found that bovine norovirus (BNoV) is one of the principal viruses causing diarrhoea in calves in Xinjiang, China. Material and Methods A total of 974 calf faecal samples from six regions in Xinjiang were tested for BNoV using reverse-transcriptase PCR. The genomic characteristics of BNoV and the genetic
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The first confirmed cases of pigeon rotavirus A (RVA) infection in domestic pigeons (Columba livia) in Poland J. Vet. Res. (IF 1.8) Pub Date : 2024-02-19 Krzysztof Adamczyk, Dennis Rubbenstroth, Aleksandra Ledwoń, Rafał Sapierzyński, Piotr Szeleszczuk
Introduction Although the presence of rotaviruses in pigeon samples has been reported since the 1980s, its importance as an aetiological agent of the “classical” young pigeon disease (YPD) was not proven until 2020, when the Henle–Koch postulates were confirmed for pigeon-type rotavirus A (RVA) genotype G18P(17). Material and Methods From 2011 to 2020, archived liver samples from 117 pigeons submitted
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Occurrence of herpesvirus in fish J. Vet. Res. (IF 1.8) Pub Date : 2024-02-13 Sven Michael Bergmann, Yingying Wang, Yingying Li, Qing Wang, Sandro Klafack, Yeonhwa Jin, Arndt Christian Hofmann, Jolanta Kielpinska, Anna Maria Becker, Weiwei Zeng
Introduction Herpesviruses are common agents in animals of the aquatic environment. They infect many species of fish but only lead to disease in one or two species. Nevertheless, infected fish without clinical symptoms can actively transfer infectious agents to disease-susceptible species. The aim of the study was to identify and prove the natural presence of different herpesviruses. Material and Methods
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Antibiotic resistance of Gallibacterium anatis biovar haemolytica isolates from chickens J. Vet. Res. (IF 1.8) Pub Date : 2024-02-09 Olimpia Kursa, Grzegorz Tomczyk, Agata Sieczkowska, Anna Sawicka-Durkalec
Introduction Gallibacterium anatis is an opportunistic bacteria inducing a range of clinical signs in poultry. Gallibacterium anatis strains show multidrug resistance to antibacterial substances. The purpose of this study was to examine the susceptibility of G. anatis biovar haemolytica isolates collected from the respiratory, reproduction and gastrointestinal tracts of chickens to different antibiotics
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The activity of monocyte-derived macrophages after stimulation with platelet-rich and platelet-poor concentrates. Study on an ovine model of insertion of a tibial implant coated with silicon-doped diamond-like carbon J. Vet. Res. (IF 1.8) Pub Date : 2024-01-30 Bartłomiej Szymczak, Andrzej Junkuszew, Krzysztof Patkowski, Tomasz Szponder, Dominika Nguyen Ngoc, Beata Drzewiecka, Aleksandra Sobczyńska-Rak, Joanna Wessely-Szponder
Introduction Macrophages are crucial immune cells that play a role in tissue repair and can exhibit pro- or anti-inflammatory behaviour based on environmental stimulation. Their functional phenotype can be affected by platelet-derived products as determined by those products’ composition. When the inflammatory response caused by implantation is excessive, it can lead to rejection of the implant. Therefore
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Microbiological analysis of skin lesions of cod (Gadus morhua) from the southern part of the Baltic Sea J. Vet. Res. (IF 1.8) Pub Date : 2024-01-25 Agnieszka Pękala-Safińska, Katarzyna Nadolna-Ałtyn, Mirosław Różycki, Ewa Paździor, Tomasz Cencek, Magdalena Podolska
Introduction Since the middle of the 1980s, severe skin disorders have been observed in Baltic cod (Gadus morhua) each year. Available data on the spectrum of bacteria isolated from the clinical cases being limited, and evaluation of the microbial background of fish skin lesions being useful, a bacteriological examination has been undertaken. Material and Methods A total of 1,381 cod were caught during
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Development of a method for the determination of sedatives in bovine and porcine urine and kidneys by liquid chromatography–tandem mass spectrometry J. Vet. Res. (IF 1.8) Pub Date : 2024-01-20 Tomasz Śniegocki, Elżbieta Samorek, Bartosz Sell, Weronika Krajewska
Introduction Sedatives have been used for a long time as animal tranquillisers to prevent stress and weight loss during their transportation. The proper determination of these substances in food of animal origin is essential for consumer safety. Material and Methods A 1 g portion of pig or cow urine or homogenised kidney was mixed with acetonitrile, sodium chloride was added, and the solution was further
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Cytokine inflammatory response in dairy cows with mastitis caused by Streptococcus agalactiae J. Vet. Res. (IF 1.8) Pub Date : 2024-01-20 Mariola Bochniarz, Agata Hahaj-Siembida, Monika Krajewska-Wędzina, Marcelina Osińska, Anna Tracz, Aleksandra Trościańczyk, Piotr Brodzki, Leszek Krakowski, Urszula Kosior-Korzecka, Aneta Nowakiewicz
Introduction The aim of the study was evaluation of the concentrations of interleukin (IL)-1β, IL-8, IL-12β and tumour necrosis factor alpha (TNF-α) in the serum and milk of cows with mastitis caused by Streptococcus agalactiae. Material and Methods A total of 60 milk samples from diseased cows and 30 milk samples from healthy cows were included in the study. Blood and milk samples were taken from
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Development of a recombinant protein-based ELISA for detection of antibodies against bovine herpesvirus 6 (BoHV6) J. Vet. Res. (IF 1.8) Pub Date : 2023-12-20 Piotr Kubiś, Jacek Kuźmak
Introduction Bovine herpesvirus 6 (BoHV6) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily and Macavirus genus. It is common in cattle, but was also detected in American bison (Bison bison) and water buffalo (Bubalus bubalis). The aim of the experiment was to develop an ELISA for serological examination of cattle sera for the presence of anti-BoHV6 specific antibodies. Material and
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Development, in-house validation and application of a method using high-performance liquid chromatography with fluorescence detection (HPLC-FLD) for the quantification of 12 ergot alkaloids in compound feeds J. Vet. Res. (IF 1.8) Pub Date : 2023-12-20 Ewelina Kowalczyk, Krzysztof Kwiatek
Introduction Ergot alkaloids (EAs) are toxic substances naturally produced by Claviceps fungi. These fungi infest a wide range of cereals and grasses. When domestic animals are exposed to EAs through contaminated feeds, it is detrimental to them and leads to significant economic losses. For that reason, it is important to monitor feed for the presence of EAs, especially with methods enabling their
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Why does Listeria monocytogenes survive in food and food-production environments? J. Vet. Res. (IF 1.8) Pub Date : 2023-12-04 Jacek Osek, Kinga Wieczorek
Listeria monocytogenes is one of the most dangerous food-borne pathogens and is responsible for human listeriosis, a severe disease with a high mortality rate, especially among the elderly, pregnant women and newborns. Therefore, this bacterium has an important impact on food safety and public health. It is able to survive and even grow in a temperature range from -0.4°C to 45°C, a broad pH range from
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Immunoreactivity of p21, MMP-1 and CB2 receptor proteins in cutaneous canine mast cell tumours: an association with the three-tier grading system J. Vet. Res. (IF 1.8) Pub Date : 2023-12-04 Kamila Bulak, Anna Kycko, Anna Śmiech, Wojciech Łopuszyński
Introduction Mast cell tumours (MCTs) arise in the dermis and subcutaneous tissues in animals and humans and are one of the most common neoplasms of the skin in dogs. Cannabinoid type 2 receptor (CB2R), cyclin-dependent kinase inhibitor (p21) and matrix metalloproteinase 1 (MMP-1) are potential targets for novel anti-tumour therapeutic strategies. This study evaluated by immunohistochemical means the
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New insight on chlamydiae J. Vet. Res. (IF 1.8) Pub Date : 2023-12-04 Monika Szymańska-Czerwińska, Kinga Zaręba-Marchewka, Krzysztof Niemczuk
This article provides an overview of the current knowledge on chlamydiae, which are intracellular bacteria belonging to the Chlamydiaceae family. Whole-genome sequencing leads to great increases in the available data about Chlamydia spp. Recently, novel chlamydial taxons in various hosts living in different environments have been recognised. New species and taxons with Candidatus status have been recorded
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Molecular contamination of an animal facility during and after African swine fever virus infection J. Vet. Res. (IF 1.8) Pub Date : 2023-11-20 Marek Walczak, Krzesimir Szymankiewicz, Fernando Rodriguez, Jordi Argilaguet, Boris Gavrilov, Jacek Żmudzki, Maciej Kochanowski, Małgorzata Juszkiewicz, Anna Szczotka-Bochniarz
Introduction The molecular contamination of an animal facility was investigated during and after an infection with highly pathogenic African swine fever virus (ASFV) among domestic pigs. The investigation evaluated the risk of indirect transmission of the disease and indicated points that may facilitate cleaning and disinfection processes. Material and Methods Six domestic pigs were infected oronasally
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The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes J. Vet. Res. (IF 1.8) Pub Date : 2023-11-20 Monika Olech
Introduction Maedi-visna virus and caprine arthritis encephalitis virus are two closely related lentiviruses which cause multisystemic, progressive and persistent infection in goats and sheep. Because these viruses frequently cross the species barrier, they are considered to be one genetic group called small-ruminant lentiviruses (SRLV). They have in vivo tropism mainly for monocytes and macrophages
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The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK J. Vet. Res. (IF 1.8) Pub Date : 2023-11-17 Zbigniew Arent, Colm Gilmore, Laura Pardyak, Klaudia Dubniewicz, Barry McInerney, William Ellis
Introduction Strains of Leptospira interrogans belonging to two very closely related serovars, Icterohaemorrhagiae and Copenhageni, have been associated with disease in mammalian species and are the most frequently reported agents of human leptospirosis. They are considered the most pathogenic serovars and represent more than half of the leptospires encountered in severe human infections. Material
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Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland J. Vet. Res. (IF 1.8) Pub Date : 2023-11-08 Sebastian Smulski, Marcin Pszczoła, Monika Stachowiak, Adrianna Bilińska, Izabela Szczerbal
Introduction MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows. Material and Methods Milk samples (n = 90) were collected from healthy and mastitic dairy cows originating from local dairy cattle farms located
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Study on the expression of testin in the testes of dogs J. Vet. Res. (IF 1.8) Pub Date : 2023-10-31 Rafał Ciaputa, Marcin Nowak, Stanisław Dzimira, Eleonora Brambilla, Małgorzata Kandefer-Gola, Alicja Tomaszek, Aneta Popiel-Kopaczyk, Piotr Dzięgiel, Valeria Grieco
Introduction Testin is a protein involved in cell mobility, adhesion and colony formation. In rats, testin presence has been reported in the testes, and its possible role in spermatogenesis has been suggested. Studies in humans also suggest a possible role of testin as a cancer suppressor protein. In the dog, which represents both an important pet species and a good animal model for studying biological
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Enterovirus E infects bovine peripheral blood mononuclear cells. Implications for pathogenesis? J. Vet. Res. (IF 1.8) Pub Date : 2023-10-24 Joanna Małaczewska, Małgorzata Wróbel, Edyta Kaczorek-Łukowska, Wojciech Rękawek
Introduction Enterovirus E (EV-E) is a common viral pathogen endemic in cattle worldwide. Little is known, however, about its potential interactions with bovine immune cells. Material and Methods The EV-E-permissiveness of bovine peripheral blood mononuclear cells (PBMCs) was evaluated. The infectious titres of extracellular virus were measured and the intracellular viral RNA levels were determined
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The use of plant extracts and bacteriophages as an alternative therapy approach in combatting bacterial infections: the study of lytic phages and Stevia rebaudiana J. Vet. Res. (IF 1.8) Pub Date : 2023-10-24 Xymena Stachurska, Małgorzata Mizielińska, Magdalena Ordon, Paweł Nawrotek
Introduction In the light of the problem of antibiotic resistance, the use of combined alternative therapies in combatting bacteria-related disorders has gained popularity. Bacteriophages are one element implemented in new combination therapy. Stevia rebaudiana is known to have antimicrobial activity and regarded as potentially having a synergistic effect with bacteriophages. Therefore, possible interactions
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Some aspects of the diagnosis and treatment of eosinophilic granuloma in cats J. Vet. Res. (IF 1.8) Pub Date : 2023-10-19 Hanna Omelchenko, Natalia Avramenko, Serhii Kulynych, Maksym Petrenko, Vladyslav Volosovets, Natalia Volosovets, Grzegorz Woźniakowski
Introduction Eosinophils represent the most active cells in mammals that show protective and assistive activity in the host immune defence against helminth parasites. These cells are also responsible for the reduction of allergic and inflammatory reactions. The eosinophils play a key role in allergic reactions by secretion of different chemical molecules leading to swelling, lesions and granuloma onset
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Multidrug resistance in Haemonchus contortus in sheep - can it be overcome? J. Vet. Res. (IF 1.8) Pub Date : 2023-10-17 Michal Babják, Alžbeta Königová, Michaela Komáromyová, Tetiana Kuzmina, Pawel Nosal, Marián Várady
Introduction Gastrointestinal nematodes pose a threat to animal health and affect farmers by negatively impacting farm management. Material and Methods The study was conducted on a sheep farm with suspected reductions in the efficacies of anthelmintics. Efficacy was determined using in vivo faecal egg count reduction, in vitro egg hatch (EHT) and larval development (LDT) tests. In the first phase,
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Occurrence of perfluoroalkyl substances in cow’s, goat’s and sheep’s milk – dietary intake and risk assessment J. Vet. Res. (IF 1.8) Pub Date : 2023-10-13 Szczepan Mikołajczyk, Małgorzata Warenik-Bany, Marek Pajurek
Introduction Milk from cows, goats and sheep was analysed in terms of content of fourteen perfluoroalkyl substances (PFASs). Material and Methods Altogether, 73 milk samples from cows (n = 38), goats (n = 20) and sheep (n = 15) were collected from various regions of Poland. Concentrations of analytes were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS). Results The lower-bound
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Echinococcus multilocularis genetic diversity based on isolates from pigs confirmed the characteristic haplotype distribution and the presence of the Asian-like haplotype in Central Europe J. Vet. Res. (IF 1.8) Pub Date : 2023-10-10 Jacek Karamon, Małgorzata Samorek-Pieróg, Ewa Bilska-Zając, Weronika Korpysa-Dzirba, Jacek Sroka, Aneta Bełcik, Jolanta Zdybel, Tomasz Cencek
Introduction The aim of the study was to determine the genetic diversity of Echinococcus multilocularis in pigs in highly endemic areas in Poland, as well as to attempt to confirm the occurrence and geographical distribution of haplotypes characteristic for these areas, which were previously described on the basis of examination of adult tapeworms isolated from foxes. Material and Methods Twenty samples
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Morphometry and topography of the coronary ostia in the dog J. Vet. Res. (IF 1.8) Pub Date : 2023-09-21 Karolina Barszcz, Karolina Goździewska-Harłajczuk, Michał Czopowicz, Magdalena Chłopecka, Michał Polguj, Joanna Klećkowska-Nawrot
Introduction The purpose of this study was to perform a morphometric examination of the coronary ostia, including their location in the area of the aortic sinuses, and to describe variations in ostia structure in the domestic dog. Material and Methods The study was conducted on the hearts of 91 pedigree dogs of both sexes, aged 1 to 18 years (median 9 years), with a body weight from 1.2 to 65 kg (median
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Molecular identification of blaTEM and blaCTX-M genes in multidrug-resistant Escherichia coli found in milk samples from dairy cattle farms in Tulungagung, Indonesia J. Vet. Res. (IF 1.8) Pub Date : 2023-09-21 Agus Widodo, Mirni Lamid, Mustofa Helmi Effendi, Wiwiek Tyasningsih, Dadik Raharjo, Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Lita Rakhma Yustinasari, Katty Hendriana Priscilia Riwu, Otto Sahat Martua Silaen
Introduction Escherichia coli is an opportunistic bacteria that can grow easily, produce toxins, and resist antibiotics. The phenomenon of E. coli developing multidrug resistance is currently the subject of extensive research. The objective of this study was to molecularly identify blaTEM and blaCTX-M genes in multidrug-resistant E. coli found in milk samples from dairy cattle farms in Tulungagung
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Possibilities of using mussels (Mytilus galloprovincialis) to predict rotavirus contamination in Albania J. Vet. Res. (IF 1.8) Pub Date : 2023-09-21 Arta Lugaj, Kastriot Korro, Gianfranco Santovito
Introduction Rotaviruses are non-enveloped viruses that each consist of 11 double-stranded RNA molecules. These viruses are able to persist in the environment, and therefore play a fundamental role in the epidemiology of gastroenteritis and severe diarrhoea in children worldwide. While mussels have been primarily used as indicators of chemical pollution, they can also be used to monitor viral contamination
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Distribution of carp edema virus in organs of infected juvenile common carp J. Vet. Res. (IF 1.8) Pub Date : 2023-09-01 Marek Matras, Magdalena Stachnik, Ewa Borzym, Joanna Maj-Paluch, Michał Reichert
Introduction The disease caused by carp edema virus (CEV) manifests with lethargy as a primary sign; this observation in koi in Japan gained the disease the name koi sleepy disease (KSD). In the years following the discovery of the virus in Japan, KSD cases have been noted in the UK in koi and common carp. Conducting research in order to expand knowledge of the processes of distribution of CEV in infected
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Comparison of automatic methods MALDI-TOF, VITEK2 and manual methods for the identification of intestinal microbial communities on the example of samples from alpacas (Vicugna pacos) J. Vet. Res. (IF 1.8) Pub Date : 2023-09-01 Joanna Pławińska-Czarnak, Karolina Wódz, Zuzanna Strzałkowska, Monika Żychska, Tomasz Nowak, Adam Kwieciński, Piotr Kwieciński, Wojciech Bielecki, Anna Rodo, Magdalena Rzewuska, Daria Kłosińska, Krzysztof Anusz, Blanka Orłowska
Introduction Universally, in microbiological diagnostics the detection of live bacteria is essential. Rapid identification of pathogens enables appropriate remedial measures to be taken. The identification of many bacteria simultaneously facilitates the determination of the characteristics of the accompanying microbiota and/or the microbiological complexity of a given environment. Material and Methods
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Antimicrobial resistance of Listeria monocytogenes serogroups IIa and IVb from food and food-production environments in Poland J. Vet. Res. (IF 1.8) Pub Date : 2023-08-31 Beata Lachtara, Kinga Wieczorek, Jacek Osek
Introduction Listeria monocytogenes is an important foodborne pathogen responsible for human listeriosis, which is a disease with high hospitalisation and mortality rates. The bacteria are usually susceptible to most antibacterial substances, but resistance to some of them has been recently observed. The present study introduces the evidence on the emergence of antibiotic resistance among L. monocytogenes
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Incidence and the risk of occurrence of benign and malignant canine skin tumours in Poland – a five-year retrospective study J. Vet. Res. (IF 1.8) Pub Date : 2023-08-31 Anna Śmiech, Kamila Bulak, Wojciech Łopuszyński, Agata Puła
Introduction The aim of the study was to compile data on the frequency and distribution of canine skin tumours and determine the risk of these being malignant as opposed to benign. This determination proceeded from tumour histogenesis and gave consideration to the dog’s breed, sex, age and the anatomical location of tumours. Material and Methods This retrospective five-year epidemiological study included
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Porcine carcasses as an underestimated source of antimicrobial resistant Campylobacter coli J. Vet. Res. (IF 1.8) Pub Date : 2023-08-31 Jacek Osek, Kinga Wieczorek
Introduction Campylobacteriosis is the most common human foodborne bacterial infection worldwide and is caused by bacteria of the Camplylobacter genus. The main source of these bacteria is poultry, but other food-producing animals such as pigs are also responsible for human infections. An increasing number of strains with resistance to fluoroquinolones and other antimicrobials such as macrolides were
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An initial characterisation of the Unfolded Protein Response pathway in haematopoietic canine cancer cell lines – a necessary step for the future development of new therapies in dogs with neoplasia J. Vet. Res. (IF 1.8) Pub Date : 2023-08-31 Beatriz Hernández-Suárez, David A. Gillespie, Bożena Obmińska-Mrukowicz, Aleksandra Pawlak
Introduction New and more effective therapies for canine cancer patients are urgently required and this necessitates advanced experimental research. Dogs are good models for studies in comparative oncology; however, canine cancer cell biology research is currently limited by low availability of validated antibody reagents and techniques. This study characterises the expression of key components of
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Can the examination of different types of hive samples be a non-invasive method for detection and quantification of viruses in honey bee (Apis mellifera L.) colonies? J. Vet. Res. (IF 1.8) Pub Date : 2023-08-31 Eliška Čukanová, Jana Prodělalová, Miroslava Palíková, Kristýna Kováčová, Petr Linhart, Ivana Papežíková
Introduction Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (Apis mellifera L). In the present work, beehive materials were tested for their potential to serve as non-invasive samples for honey bee virus detection. Material and Methods Honey, pollen, hive debris, hive grid smears and forager honey bees were collected from 24 hives at four locations
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Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats J. Vet. Res. (IF 1.8) Pub Date : 2023-07-25 Ashraf M. Morgan, Hanan A. Ogaly, Shaimaa Kamel, Maha M. Rashad, Eman I. Hassanen, Marwa A. Ibrahim, Mona K. Galal, Aya M. Yassin, Sharah A. Al Dulmani, Fatimah A.M. Al-Zahrani, Ahmed M. Hussien
Introduction Penconazole (PEN) is a widely applied triazole fungicide. This study sought to define the efficacy of N-acetyl-l-cysteine (NAC) in mitigating PEN-triggered hepatorenal toxicity in rats. Material and Methods Twenty-eight adult male albino Wistar rats were assigned to four groups: a normal control (NC), a PEN group, a NAC group and a PEN+NAC group. Administration of PEN (50 mg/kg body weight
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Inter-laboratory validation of liquid chromatography–tandem mass spectrometry multi-mycotoxin determination in animal feed – method transfer from the reference laboratory to regional laboratories J. Vet. Res. (IF 1.8) Pub Date : 2023-07-25 Piotr Jedziniak, Łukasz Panasiuk, Katarzyna Pietruszka, Krzysztof Kolano, Radosław Zbytniewski
Introduction The results are presented of the inter-laboratory validation of a liquid chromatography–tandem mass spectrometry method for the determination of eight mycotoxins (aflatoxin B1, deoxynivalenol, fumonisin B1, fumonisin B2, ochratoxin A, toxin T-2, toxin HT-2 and zearalenone) in animal feeds. Material and Methods This study was an essential part of the method’s transfer from the National
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Utilisation of Actiphage in combination with IS900 qPCR as a diagnostic tool for rapid determination of paratuberculosis infection status in small ruminant herds J. Vet. Res. (IF 1.8) Pub Date : 2023-07-21 Monika Beinhauerova, Iva Slana
Introduction Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a chronic infectious intestinal disease occurring in domestic and wild ruminants. Early diagnosis of infected herds enabling timely adoption of control measures is tremendously important in view of the fact that the disease has a significant economic impact on farmers. The aim of this study was
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Examination of the haematological profile of pregnant Polish Holstein-Friesian black-and-white cattle in the early stage J. Vet. Res. (IF 1.8) Pub Date : 2023-07-21 Marcjanna Wrzecińska, Alicja Kowalczyk, Ewa Czerniawska-Piątkowska, Władysław Kordan, Jose Pedro Araujo
Introduction Cattle health and welfare are monitored via the analysis of the haematological profile, and it shows cattle’s ability to adapt to changing environmental conditions, pregnancy and lactation; profile changes also indicate reproductive disorders. The literature lacks reports of the examination of the haematological profile in cows up to the 50th day of pregnancy (dop). Therefore, this research
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Levels of caesium-137 in food of animal origin in Poland J. Vet. Res. (IF 1.8) Pub Date : 2023-07-20 Magdalena Gembal, Paweł Czerski, Ewelina Milczarczyk, Małgorzata Warenik-Bany
Introduction Radioactive contamination of the environment is one of the greatest threats after a nuclear accident due to released radionuclides. From a radiotoxicological point of view, the most important radionuclide is caesium-137. Formed mainly during nuclear explosions, caesium-137 can persist in the soil for many years, from where it constantly enters the food chain. One of the elements of ensuring
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Identification of SNP markers for canine mammary gland tumours in females based on a genome-wide association study – preliminary results J. Vet. Res. (IF 1.8) Pub Date : 2023-07-10 Anna Mucha, Błażej Nowak, Stanisław Dzimira, Bartłomiej Liszka, Magdalena Zatoń-Dobrowolska
Introduction The development of genetic research over recent decades has enabled the discovery of new genetic markers, such as single nucleotide polymorphisms (SNPs). This, as well as the full sequencing of the dog genome, has enabled genome-wide association studies (GWAS) to be used in the search for genetic causes of canine mammary tumours (CMTs). Material and Methods Genotypic data containing 175
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Antibody response to 1.0 and 0.5 mL doses of an inactivated bacterial vaccine against bovine respiratory disease in young Holstein calves: a field trial J. Vet. Res. (IF 1.8) Pub Date : 2023-07-04 Kazusa Mori, Toshihide Kato, Keigo Kosenda, Osamu Yokota, Hiromichi Ohtsuka
Introduction Early vaccination of cattle with an inactivated commercial bacterial vaccine against bovine respiratory disease has been reported to increase antibody production and can alleviate the disease. However, its dosage has been little investigated in young Holstein calves. This study addresses the need to establish guide values for vaccine dosage in these animals. Material and Methods Healthy
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First molecular detection of Brachyspira suanatina on pig farms in Poland J. Vet. Res. (IF 1.8) Pub Date : 2023-06-27 Piotr Cybulski, Katrin Strutzberg-Minder, Edyta Michalik, Radosław Kondratiuk, Artur Jablonski
Introduction Prior to the 2000s, swine dysentery was considered to be caused only by Brachyspira hyodysenteriae with contributing commensal intestinal anaerobes. Nowadays, it is known that the disease is caused by three strongly beta-haemolytic species of the anaerobic spirochaetal genus Brachyspira, i.e. B. hyodysenteriae and newly emerged B. hampsonii and B. suanatina. Material and Methods The present
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Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia J. Vet. Res. (IF 1.8) Pub Date : 2023-06-17 Jelena Maksimović Zorić, Ljubiša Veljović, Vladimir Radosavljević, Dimitrije Glišić, Jasna Kureljušić, Jelena Maletić, Božidar Savić
Introduction Swine influenza A viruses (swIAVs) are characterised by high mutation rates and zoonotic and pandemic potential. In order to draw conclusions about virulence in swine and pathogenicity to humans, we examined the existence of molecular markers and accessory proteins, cross-reactivity with vaccine strains, and resistance to antiviral drugs in five strains of H1N1 swIAVs. Material and Methods
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Molecular epidemiological characteristics and genetic evolutionary relationships of methicillin-resistant Staphylococcus aureus of different avian origins in Qingdao, China, using whole-genome sequencing J. Vet. Res. (IF 1.8) Pub Date : 2023-06-17 Zhen Xiao, Zhina Qu, Na Liu, Juan Wang, Jianmei Zhao, Junhui Liu, Lin Wang, Xiumei Huang, Qingqing Zhang, Yubin Gao, Junwei Wang, Zhiyong Yu, Jiajia Guan, Huanqi Liu
Introduction To understand the prevalence of avian methicillin-resistant Staphylococcus aureus (MRSA) and the current status of drug resistance in Qingdao, a comprehensive molecular epidemiological investigation and analysis of evolutionary relationships of MRSA isolates from broiler and layer chickens and waterfowl was conducted. Material and Methods One hundred and two avian MRSA strains were identified
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Antioxidant and antimicrobial properties of an extract rich in proteins obtained from Trametes versicolor J. Vet. Res. (IF 1.8) Pub Date : 2023-06-17 Katarzyna Michalak, Stanisław Winiarczyk, Łukasz Adaszek, Urszula Kosikowska, Sylwia Andrzejczuk, Katarzyna Garbacz, Anna Dobrut, Łukasz Jarosz, Wiktoria Czupryna, Dorota Pietras-Ożga
Introduction Bioactive proteins and peptides generated from fruit, vegetables, meat or fish have great potential as functional food or substitutes for antibiotics. In recent years it has also been demonstrated that the fungus kingdom could be a source of these compounds. The study investigated the bioactivity of an extract of the lignicolous fungus Trametes versicolor and its hydrolysate. Material
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Liquid chromatographytandem mass spectrometry methods for determination of stanozolol and l6β-hydroxy-stanozolol in animal urine J. Vet. Res. (IF 1.8) Pub Date : 2023-06-02 Iwona Matraszek-Źuchowska, Alicja Kłopot, Katarzyna Sielska, Beata Korycińska, Sebastian Witek, Paulina Zdonek, Piotr Jedziniak
Introduction Because of the activities and effects they induce, hormones are prohibited for use for anabolic purposes in farm animals intended for slaughter, which is regulated in the European Union by relevant legal provisions. Therefore, there is an obligation to monitor residues of hormones in animals and food of animal origin to ensure consumer safety. A hormone banned but used formerly for fattening
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Comparative analysis of total protein, casein, lactose, and fat content in milk of cows suffering from subclinical and clinical mastitis caused by Streptococcus spp J. Vet. Res. (IF 1.8) Pub Date : 2023-05-31 Mariola Bochniarz, Przemysław Błaszczyk, Marek Szczubiał, Iosif Vasiu, Łukasz Adaszek, Katarzyna Michalak, Dorota Pietras-Ożga, Marco Wochnik, Roman Dąbrowski
Introduction The aim of the study was to analyse the total protein (TP), casein (CAS), lactose (LAC), and fat content of milk from cows with subclinical (SCM) and clinical mastitis (CM) caused by Streptococcus spp. Material and Methods A total of 60 milk samples from diseased cows and 30 milk samples from healthy cows were included in the study. Milk samples were taken from Holstein-Friesian cows from
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Monitoring of antibiotic use in broiler turkey flocks in the Warmia and Mazury province in 2019–2021 J. Vet. Res. (IF 1.8) Pub Date : 2023-05-31 Marcin Śmialek, Teresa Konieczka, Paweł Konieczka, Joanna Kowalczyk, Andrzej Koncicki, Krzysztof Kozłowski, Jan Jankowski
Introduction The increasing resistance of bacteria to antibiotics has obliged the EU Member States to reduce by 50% the use of antibiotics in animal production by 2030. This study was undertaken with the aim to analyse the use of antibiotics in flocks of broiler turkeys reared in the Warmia and Mazury province in a two-year period. Material and Methods From data from 238 production records of turkey
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Effects of long-term administration of various dietary prebiotic supplements on the growth, immune cell activity and digestive tract histology of juvenile vimba (Vimba vimba) J. Vet. Res. (IF 1.8) Pub Date : 2023-05-31 Barbara Kazuń, Krzysztof Kazuń, Joanna Małaczewska, Rafał Kamiński, Dobrochna Adamek-Urbańska, Justyna Sikorska, Jacek Wolnicki, Hubert Szudrowicz
Introduction The experiment was set to determine the effects of long-term (55-day) use of three commercial prebiotics including Saccharomyces cerevisiae–derived β-glucans and one including inulin on juvenile vimba (Vimba vimba) reared intensively under controlled conditions. Material and Methods Six-month-old fish were fed commercial feed (Control group, n = 90), or the same feed supplemented with
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Molecular characterisation and antimicrobial resistance of Streptococcus agalactiae isolates from dairy farms in China J. Vet. Res. (IF 1.8) Pub Date : 2023-05-23 Xiaojiao Ma, He Chen, Fulan Wang, Shuai Wang, Yating Wu, Xianlan Ma, Yong Wei, Wei Shao, Yankun Zhao
Introduction Streptococcus agalactiae (S. agalactiae) is a pathogen causing bovine mastitis that results in considerable economic losses in the livestock sector. To understand the distribution and drug resistance characteristics of S. agalactiae from dairy cow mastitis cases in China, multilocus sequence typing (MLST) was carried out and the serotypes and drug resistance characteristics of the bacteria
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TaqMan real-time PCR for detection of pathogenic Leptospira spp. in canine clinical samples J. Vet. Res. (IF 1.8) Pub Date : 2023-05-23 Mohammad Sabri Abdul Rahman, Kuan Hua Khor, Siti Khairani-Bejo, Seng Fong Lau, Mazlina Mazlan, Mohd Azri Roslan, Mohd Mokrish Md Ajat, Mohd Akmal Mohd Noor
Introduction Canine leptospirosis has always been a differential diagnosis in dogs presenting with clinical signs and blood profiles associated with kidney and/or liver disease. The conventional polymerase chain reaction (PCR) provides diagnoses, but real-time PCR-based tests provide earlier confirmation and determine the severity of infection, especially in the acute stage, allowing early detection
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Effects of Punica granatum L. peel extract supplementation on body weight, cardiac function, and haematological and biochemical parameters in an animal model of metabolic syndrome J. Vet. Res. (IF 1.8) Pub Date : 2023-05-23 Joanna Niewiadomska, Ewa Kumiega, Michał Płóciennik, Jacek Gajek, Agnieszka Noszczyk-Nowak
Introduction Metabolic syndrome (MetS) is a cluster of pathological conditions well described in humans but still investigated insufficiently in animals. A novel approach in its management is the utilisation of nutrients from natural sources. Recent studies suggested that phenolic compounds from pomegranate peel could be a promising dietary intervention for MetS. This study evaluated the potency of
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Prevalence of the bla CTX-M and bla TEM genes among extended-spectrum beta lactamase–producing Escherichia coli isolated from broiler chickens in Indonesia J. Vet. Res. (IF 1.8) Pub Date : 2023-05-22 Hayyun Durrotul Faridah, Freshindy Marissa Wibisono, Freshinta Jellia Wibisono, Nabilatun Nisa, Fatimah Fatimah, Mustofa Helmi Effendi, Emmanuel Nnabuike Ugbo, Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Otto Sahat Martua Silaen
Introduction Infections of humans and animals by multidrug resistant bacteria are increasing because of the inappropriate use of antibiotics. Disease management may be more challenging if Escherichia coli produce extended-spectrum beta-lactamase (ESBL), which could cause resistance to aztreonam and third-generation cephalosporins. This study was aimed at determining the prevalence of the bla CTX-M
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Diclofenac and dexamethasone modulate the effect of cannabidiol on the rat colon motility ex vivo J. Vet. Res. (IF 1.8) Pub Date : 2023-05-22 Magdalena Chłopecka, Łukasz Kiraga, Kijan Crowley, Michał Jank, Urszula Latek, Marta Mendel, Wojciech Karlik
Introduction Due to the growing interest in the use of cannabinoids in supportive therapies, they are increasingly used together with anti-inflammatory drugs. Cannabinoids inhibit gastrointestinal motility, while steroidal and nonsteroidal anti-inflammatory drugs influence motility in other ways. The aim of the research was to study the interactions between cannabidiol (CBD) and these two classes of