In this trial, nine diets were formulated to investigate the effects of DL-methionine (DL-Met) and a methionine hydroxy analogue (MHA-Ca) on growth, amino acid profiles and the expression of genes related to taurine and protein synthesis in common carp. The basal diet was prepared without methionine supplementation and the other diets were formulated with 0.1%, 0.2%, 0.3% and 0.4% of DL-Met or MHA-Ca

An 8-week experimental trial was designed to explore the effects of dietary protein level (DPL) and salinity on the growth performance and physiological parameters of genetically improved farmed tilapia (GIFT) juveniles (Oreochromis niloticus). Six diets were formulated with different dietary protein levels (18.20%, 24.32%, 30.60%, 36.42%, 43.27% and 49.49%) and two salinity levels (0and 8‰) in a 6 × 2

Zhikong scallop (Chlamys farreri Jones et Preston 1904) is one of the most economically important shellfish in the aquaculture industry of China. Improving thermal tolerance of the scallop by breeding has become a critical task for the sustainable farming industry. The detection of the majority of the current indicators of the thermal tolerance of the scallop is generally short-term, low-throughput

This study evaluated the effects of the essential oil of Ocimum gratissimum L. (EOOG) on water quality, blood characteristics and tissue damage during transport of Lophiosilurus alexandri. One hundred and sixty juveniles of L. alexandri (123.44 ± 1.95 g and 23.71 ± 1.95 cm) were fasted for 24 h before transportation. Fish were then submitted to one of three treatments of transport water with five replicates

This study was performed as part of the effort to develop artificial seed production techniques for stone flounder Kareius bicoloratus, which has a great potential for farming in northeast Asian countries. To develop a sperm cryopreservation protocol that can be applied for artificial fertilization, we carried out experiments on the key factors for successful fish sperm cryopreservation, including

Long-term storage of spermatogonial stem cells (SSCs) represents the next step for advancing aquaculture research by providing valuable genetic resources for genetic enhancement programs and xenogenesis technologies. Hybrid catfish, the cross between channel catfish, Ictalurus punctatus ♀ and blue catfish, I. furcatus ♂, are in high demand by the US aquaculture industry, but production can be limited

Nervous necrosis virus (NNV) affects more than 177 species of cultured fish including seven band grouper. The present study evaluated the shedding and transmission kinetics of NNV in seven band groupers using a cohabitation challenge model. Initial infection studied by intraperitoneal (IP) injection showed shedding of NNV was acute and peaked within 3–5 days after exposure. This was followed by a post

Although Litopenaeus vannamei is the most produced and researched shrimp species worldwide, there are no published studies evaluating the use of the microbial biomass, Novacq™, in L. vannamei feeds. This study investigated the effects of supplementing Novacq™ at 100 g kg−1 in practical and commercial feeds in postlarvae 12 (PL 12) and juvenile white leg shrimp. Three experiments were carried out. In

Fish blood contains RBCs (Red blood cells) as the major cells. Unlike the absence of nucleus in mammalian RBC, fish RBC contains a nucleus. Previous researches have indicated that fish RBCs have a significant function in immune responses. However, the mechanism underlying the immune responses against bacterial infection of teleost RBCs remains enigmatic. To decipher the mechanisms, after the infection

Glycinin is a major anti-nutritional factor of soybean. Glycinin inhibits growth and impairs intestinal health in grass carp and Jian carp, but the underlying mechanisms are largely unknown. This study used grass carp to examine whether the negative influences of dietary glycinin are related to intestinal apoptosis signalling and tight junctions (TJs). The results showed that 8% dietary glycinin caused

Identifying correct stocking densities is an important concern for shrimp farming especially when intensification of shrimp production is growing in order to meet increasing demand. Increased stocking densities generate greater competition among individuals, reduced feeding efficiencies and increased water degradation. Little is known, however, about the effects of stocking density on feeding behaviour

The fat greenling, Hexagrammos otakii, is an emerging aquaculture species and is being evaluated for production in recirculating aquaculture systems (RAS). To better understand the tolerance of H. otakii to varying environmental salinity in actual aquaculture operations, the effects of salinity on survival, growth, hematological parameters, gill microstructure and gill transcriptome were investigated

Protein restriction is a less-studied nutritional strategy in aquaculture species. The present study was designed to investigate the effects of protein restriction for different durations on the growth performance, body composition, the profile of fatty acids and amino acids, and the immune response of rainbow trout for eight weeks. For this purpose, 225 juveniles (27.74 ± 0.43) were distributed in

Monascus purpureus M-32 was successfully isolated from the Chinese traditional food red mould rice, and its fermentation product was supplemented in the diet of Litopenaeus vannamei at different concentrations (T0: 0 g/kg; T1: 5 g/kg; T2: 10 g/kg and T3: 20 g/kg). After 8 weeks, the dietary administration of the M-32 fermentation product significantly decreased the FCR in groups T1 and T2 and significantly

A breeding selection program for improving inner shell color and growth was developed for Hyriopsis cumingii, to enhance purple pearl production. In freshwater pearl culture, adjusting culture water depth and mud substrate supply are routinely carried out to improve pearl quality. To improve selection decisions in different conditions, we estimated heritabilities and genotype by environment (G × E)

In this study we evaluated the productivity of an outdoor cultivation system based in secondary attachment discs (SADs) of Chondracanthus chamissoi as propagation strategy to produce and maintain a permanent thalli stock. For that, re-attachment of fronds, survival of SADs, growth rate of new erect axes, coverage and productivity were evaluated in three different artificial substrates: fiberglass plates

Grass carp reovirus (GCRV), causative agent of grass carp hemorrhagic disease, causes high mortality of grass carp and results in huge economic losses in grass carp farming. Vaccination is considered the most effective way of preventing GCRV infection and losses of grass carp. In the study, recombination pcDNA3.1-vp4 and pcDNA3.1-vp4-3 plasmids were constructed and respectively transformed into E.

The West Indian top shell Cittarium pica is a gastropod species currently regarded as threatened mainly due to overfishing of wild stocks. Substantial efforts have been invested in the experimental culture of this mollusk for resource conservation, as well for commercial production. In order to determine the suitable culture conditions for this species, the present study addressed the effect of diet

American shad (Alosa sapidissima) is one of the most vulnerable and overreacting fish; any handlings may result in serious lesions or deaths, and suspend or interfere with the gonad maturation and ovulation, eventually leading to failures in artificial hormone induced reproduction. In this study, we first reported a successful integration of maturation and natural reproduction of anadromous American

Atlantic salmon (Salmo salar) are migratory salmonids and high-end seafood, and are widely cultivated. Its growth and development process needs to experience from freshwater to seawater phases. Salinity, as a key environmental factor, affects metabolism of Atlantic salmon, which in turn greatly affects its volatile organic compound (VOC) content and taste. We used headspace-gas chromatography-ion mobility

Vibrio parahaemolyticus is a critical pathogenic bacterium for the kuruma shrimp, Marsupenaeus japonicus. Antibacterial drug application is the main method to control vibriosis in aquaculture; however, drug residues and the emergence of resistant bacteria have restricted their use. Therefore, the present study aimed to identify metabolites that are likely to play roles in the homeostasis of the intestinal

Algal seaweeds have abundant amounts of active substances and can be used as pharmaceuticals and biomedicals in aquafeeds. In this context, the powder of red macroalgae Gracilaria persica was included in the diets of Persian sturgeon at the rate of 0, 2.5, 5, and 10 g/kg to investigate its role on the growth rate, fillet colouration, haemato-biochemical indices, serum, and skin mucus immunity. The

Dietary supplementation of herbal essential oils is recommended for aquatic animals due to their growth-promoting, immune, and antioxidative potential. Hence, the present study evaluated the inclusion of menthol essential oil (MNT) at 0.1%, 0.2%, 0.3%, and 0.4% on the growth performance, digestive enzyme activity, immunity, and antioxidative responses of Nile tilapia. After eight weeks, samples were

An 8-week feeding trial was proceeded to assess the effects of yeast and yeast extract on growth performance, anti-oxidant ability and intestinal microbiota of Litopenaeus vannamei. Five diets supplemented with 0% (Control), 3% beer yeast (Y), 0.5% yeast extract (YE0.5), 1% yeast extract (YE1) and 2% yeast extract (YE2) respectively were assigned to L. vannamei (four replicates per diet, 30 shrimp

Growth performance and survival to harvest of the Akosombo strain generation 10 and the GIFT strain generation two (derived from GIFT generation 11 in Malaysia) were evaluated in Ghana. The fish were from 97 families of the Akosombo selected line (AKOS), 30 of the Akosombo control line (AKOC), and 24 of the GIFT strain (GIFT). In total, 12,043 tagged fingerlings were stocked at a density of 3 fish

Light cycle is an important factor affecting the total carotenoid content (TCC) in tissues of noble scallops Chlamys nobilis, but its regulatory mechanism is still unclear. In this study, adult polymorphic C. nobilis (Nan'ao Golden Scallop and common brown scallop) were exposed to three different light cycles (24L/0D, 12L/12D and 0L/24D) for 10 days. The expression of carotenoid related genes (Scavenger

Aquaculture requires the monitoring of several parameters simultaneously such as temperature, salinity, ammonia, potential of hydrogen, nitrogen dioxide, bromine, among others to preserve the well-being of the fish, optimize production and meet the requirements of environmental ethics. To contribute to the fulfillment of these requirements, we propose here to use a data analysis technique called principal

The goal of this study was to evaluate the anesthetic potential of an ethanolic extract of Hyptis mutabilis and its effects on behavior, biochemistry, ionic balance, and oxidative stress biomarkers of silver catfish (Rhamdia quelen) after 24-h exposure. Additionally, its chemical composition was determined by high performance liquid chromatography coupled with a diode array detector and tandem mass

Kawakawa tuna (Euthynnus affinis) is a novel target species for aquaculture in Japan and currently there is very limited knowledge on its early ontogenetic development in captivity. The ontogeny of E. affinis larvae from hatching to 20 days after hatching (DAH) was illustrated in the present study through an integrated approach of histology, biochemistry and molecular approaches. Larvae were fed enriched

The present study aimed to investigate the effects of different dietary arachidonic acid (ARA) levels on lipid metabolism, productive performance and biological indexes of striped bass exposed to different temperatures. Within this purpose, four isoproteic (44% of crude protein) and isolipidic (11% of lipid) experimental diets were formulated based on defatted meals and different lipid sources. Fish

The blue tilapia (Oreochromis aureus) is an important cultured fish worldwide, and exhibits sexual size dimorphism in favor of males, and therefore, mono-sex breeding is of great value for aquaculture. However, the lack of accurate sex-linked markers for blue tilapia has hampered the mono-sex culture in aquaculture, as well as study of sex determination in this species. In this study, WW super-females

Pacu (Piaractus mesopotamicus) belongs to the main native fish group of the continental aquaculture in South America. However, breeding programs are not available for this species. The estimation of parameters for different traits and in different environments are needed to set up optimized breeding programs Therefore, the objectives of this study were to estimate genetic parameters, to evaluate genotype

White spot syndrome virus (WSSV) is an extremely destructive virus in shrimp farming which has caused a serious economic loss to global shrimp industry. Learning its infection routes is the basis for WSSV prevention and control. The digestive tract and gill were generally considered to be the target tissues for WSSV entry into shrimp. However, whether there is any other target tissue for WSSV entry

To investigate the possible family influence on sea lice grazing of lumpfish on Atlantic salmon, ten families of lumpfish (N = 480) with a mean (± SD) weight of 54.8 ± 9.2 g were distributed among ten sea cages (5 × 5 × 5 m) each stocked with 400 Atlantic salmon with a mean (± SD) weight of 621.4 ± 9.2 g. All the ten cages were stocked with 48 lumpfish (12% stocking density). The stocking of cages

Despite the enormous potential of tilapia farming in Bangladesh, its culture has yet to become established due to socio-economic, technical and marketing constraints. Tilapia culture is mostly concentrated in the Mymensingh region where a considerable number of fish farmers are involved in production. This study was conducted in Trishal sub-district under Mymensingh district to understand and analyze

Single cell protein (SCP) is a bulk of dried cells which can also termed as bioprotein, microbial protein or biomass. SCP is produced by the microorganisms such as algae, yeast, fungi and bacteria, however, fungi and bacteria are the major producers of this protein. High production of proteins from these sources was mainly due to their fast growth rate and relatively higher protein level in their chemical

The lockdown on account of the Coronavirus disease 2019 (COVID-19) adversely impacted the food production sector including aquaculture, globally. Unfortunately, it coincided with the major shrimp farming season in India which contributes 60% of the national annual shrimp production hence the impact was substantial. An on-line survey was carried out among the stakeholders of the shrimp farming sector

Viral Nervous Necrosis (VNN) is a major threat for the European sea bass (Dicentrarchus labrax) aquaculture industry. The improvement of disease resistance through selective breeding is a promising option to reduce outbreaks. With the development of high-throughput genotyping technologies, identification of genomic regions involved in the resistance could improve the efficiency of selective breeding

In recent years, the coculture of organisms from different trophic levels has been practiced to improve water quality and nutrient consumption. In this context, this study evaluated the effect of Crassostrea stocking density on zootechnical performance and water quality of Litopenaeus vannamei reared in a multitrophic biofloc system during the nursery phase. The experimental design was completely randomized

Peptides with antimicrobial potential can now be easily and efficiently predicted, designed and validated using in silico tools. Here, twelve peptides with molecular weights between 1.6 and 1.8 kDa were predicted from the sequence of the small subunit of Penaeus vannamei hemocyanin (PvHS) using three online software (AntiBP2, CAMP and APD3). Among the twelve predicted peptides, two peptides, PvHS8

All-female populations of mandarin fish (Siniperca chuatsi) provide economic advantages in production because of the superior growth of females relative to males. The first step to produce all-female populations was artificially induced sex reversal of female mandarin fish; beginning at 10 dph, fish (undifferentiated) were treated with dietary 17α-methyltestosterone (MT) at 50 mg/kg and 100 mg/kg dosages

Taurine serves an important role in many species, including fish. The purpose of this study was to investigate the effects of varying dietary taurine levels on the growth, antioxidant capacity, and tissue deposition of taurine in golden pompano. In addition, the mechanism of taurine synthesis was studied via enzyme activity and gene expression of the following enzymes: cystathionine β-synthase (CBS)

Sperm competition, as the last form of male competition prior to fertilization, is an important component in the reproductive and evolutionary biology of polyandrous species. Golden cuttlefish (Sepia esculenta) is a commercially important annual cephalopod, in which females often mate with multiple males during their mating season and maintain viable sperm in their sperm storage organs for a long time

Phytogenics additives have gained considerable attention as efficacious and safe dietary interventions to reduce the toxic effects of aflatoxin B1 contamination in aquatic animals. The present study was conducted to investigate the ameliorative efficacy of Thymus vulgaris essential oil on aflatoxin B1 (AFB1) toxicity in rainbow trout (Oncorhynchus mykiss) in terms of growth performance, innate plasma

This study uses personal predispositions, attitudes towards food industry actors, media use and food consciousness as variables to explore trust in farm-raised and the degree to which respondents believe that aquaculture benefits their home state. The authors use a random sample mail survey of primary food shoppers in Wisconsin, a state in the Great Lakes region of the United States, where aquaculture

This study assessed both the effectiveness of a physical-mechanical rupture method and the ability of feed additives (phytogenic compounds, alginate oligosaccharide and nucleotides) to enhance the utilization of G. gracilis by European seabass. A commercial-based diet was used as control diet (CTRL) and compared with five isoproteic (53.5% Dry matter, DM) and isolipidic (14.9% DM) diets containing

Vegetable oils (VO) that are used to substitute fish oil in aquafeeds may affect, not only the fatty acid composition, but also lipid metabolism and distribution. The present study was designed to investigate this issue in juvenile golden pompano Trachinotus ovatus fed eight diets formulated with typical VO with widely varying fatty acid compositions including coconut oil (CO), palm oil (PO), oil-tea

An 8-week feeding trial was conducted to investigate the impacts of dietary phospholipid (PL) on growth, antioxidant status and lipid metabolism of Eriocheir sinensis juveniles (0.53 ± 0.01 g) fed four diets including high lipid (13%, HL) and low lipid (8%, LL) and each lipid level containing either high PL (5%, HP) or low PL (1%, LP). Final weight, weight gain, specific growth rate and molting frequency

Morphological species identification in juvenile Scylla is now possible using image analysis of frontal lobe spines. The differences found are subtle and require automation using a mobile application. Considering the bulk of crablets to be sorted for trading, distinct features that can help stakeholders distinguish the species of juveniles at a glance is still needed. In this study, morphometric analysis

Chitin isolated from Daphnia similis and its derivate, chitosan, were prepared to evaluate the effects on the improvement of growth performance of white shrimp, Penaeus vannamei. The diets containing 0.4% chitin or 0.4% chitosan were fed to shrimp for 56 days to evaluate the growth performance of shrimp. A diet without chitin or chitosan supplementation was used as the control diet (herein known as

Orange-spotted grouper (Epinephelus coioides) is one of the most important aquaculture species in tropical and subtropical Asia. Traditionally, rotifer are fed to the larvae after they hatched. However, larval survival rate has been very low, and prey of suitable size during the first week after hatching is a major constraint. In this study, we raised grouper larvae by using an inorganic fertilization

Porcine solubles, originating from porcine mucosal tissues, is a by-product of heparin production. The nutritional value and functionality of a carrier-free, spray-dried porcine soluble (DPS) product was evaluated in 56-day experiment with juvenile rice field eel Monopterus albus (initial weight: 15.5 ± 0.19 g) fed diets with optimal (42%) and a low level (22%) of fishmeal (FM) inclusion. Six isonitrogenous

In recent years, salmon aquaculture has struggled to mitigate the impact of harmful jellyfish species in northern Europe and beyond. Typically, large aggregations of jellyfish are carried by currents through salmon pens, leading to unsustainable mortality levels in some years. One potential solution that has been regularly proposed is the creation of a ‘bubble curtain’ around fish pens. A bubble curtain

Following recent advances in hatchery technology and large-scale larval rearing of spiny and slipper lobsters, a greater understanding of key nutritional requirements is now imperative to facilitate feed development for juvenile culture. However, there is a lack of relevant information available for the slipper lobsters, particularly Thenus species. This study sought to evaluate the potential requirements

This study assessed the export potential of pangasius and tilapia by considering quality standards and safety compliances of processing plants and exporters (the same companies) in Bangladesh. Primary data were collected by interviewing ten of these companies. It was found that they followed the seven principles of hazard analysis and critical control points (HACCP) as a basis of food safety management

Immune stimulators are essential to the development of the aquaculture industry. Cinnamaldehyde (CA) and Bacillus spp. are promising immunostimulatory feed additives, as demonstrated by their beneficial effects in several fish species. This study investigated the effects of dietary CA and Bacillus subtilis either alone or in combination on the growth, digestive enzyme activity, and non-specific immunity

The study examined the interactive effects of choline content (supplied as choline chloride salt (CC); no added CC, 3.0, or 6.0 g CC kg−1 diet) and water temperature (16 °C vs 24 °C) on liver lipid composition, liver histology and plasma biochemistry. Liver and plasma samples were collected from juvenile yellowtail kingfish (Seriola lalandi; YTK; 156 g body weight), fed fishmeal-based practical diets

The Pacific oyster (Crassostrea gigas) is an important bivalve in global aquaculture, and glycogen contributes greatly to the flavor and quality of these oysters. Non-coding RNAs (ncRNAs) are known to be involved in glycogen metabolism, but their impact on the oyster properties influenced by glycogen is poorly understood. We compared the expression profiles of two full-sib families with high-glycogen