The bacterial disease salmonid rickettsial septicaemia (SRS) has caused substantial economic losses in the salmonid aquaculture industry, particularly in Chile. Increasing understanding of the epidemiology of this disease is important for improving the productivity and sustainability of the industry: by identifying important influences on disease occurrence, interventions can be identified and optimised

Parasitic infestations are frequently reported in brackishwater fish. Hence, prevention and control strategy is paramount for successful brackishwater aquaculture. Emamectin benzoate (EMB) is found to be an effective in-feed treatment for controlling several ecto-parasites in fish. The objective of this study was to understand the biosafety, withdrawal and efficacy of EMB for use in brackishwater reared

Florfenicol (FLO) is the most commonly used antibiotic in aquaculture for various fish bacterial disease treatments. To ensure that low amounts of residue occur in cultured aquatic products, it is important to study the depletion of antibiotics in the process of aquaculture. Recirculating aquaculture system (RAS) is a promising aquaculture model with high water utilization that could effectively reduce

Most hatcheries use live microalgae and algae powder for artificial rearing; however, the effects of these different diets on the rearing process and bacterial communities is not clear. In order to investigate the effects of live algae and algal powder on the larval culture of Penaeus monodon. Two kinds of live algae (Chaetoceros muelleri, Thalassiosira weissflogii) and Spirulina powder were used to

Dissolved oxygen (DO) content is crucial for the development and health of aquatic animals. DO content is closely related to the antioxidant response of aquatic animals, while the interplay between DO content and the gut microbiota of aquatic animals is unclear. Thus, the objective of this study was to compare the changes in gut microbiota in Bostrichthys sinensis under normoxic (CT group) and hypoxic

The n-3 LC-PUFAs requirement was evaluated for Totoaba macdonaldi. Four isonitrogenous and isolipidic diets were formulated to contain increasing levels of n-3 LC-PUFAs (0.4%, 0.8%, 1.0%, and 1.4% of the dry diet) and fed to juvenile totoaba (1.5 g initial body weight and 47 mm initial total length) for 56 days. Growth, survival, fish whole body proximate composition, fatty acid profile, feed utilization

In an eight-week feeding trial, encapsulated sodium butyrate (ESB), gamma-aminobutyric acid (GAB), selenium-yeast (SeY), yeast extract nucleotides (YEN), yucca meal (YUM), song-gang stone (SGS) and protease (PRT) were evaluated as functional feed additives in a low fishmeal (FM) diet for olive flounder Paralichthys olivaceus. Eight experimental diets were fed to 5.26 ± 0.17 g (mean ± SD) juvenile olive

As a commercial carnassial fish, largemouth bass (Micropterus salmoides) consumes a large amount of forage fish as food annually. To reduce the use of forage fish, a formulated diet was utilized in a largemouth bass culture. Although they have been improved upon for 10 years, formulated diets still cannot meet the nutritional needs of largemouth bass. The formulated diet and forage fish were separately

Bmal1 is the key component of the central molecular clock that mediates the circadian control of various biological activities including immune reactions. In fish, the role of Bmal1 in immunity is unclear. In this study, we examined the expressional regulation and potential role of Japanese flounder Bmal1 (named PoBmal1) in pathogen associated immune response. PoBmal1 shares 85% identity with its human

Releasing hatchery-reared juveniles in the wild can mitigate the general decline in the natural stocks of European lobster, Homarus gammarus, L. However, growth and survival rates in lobster culture are low, presumably due to suboptimal nutrition and feeding. With the aim of determining appropriate nutrient levels, we tested different formulated extruded feeds for the culture of juvenile European lobster

The use of low-gossypol cottonseed protein concentrate (CPC) as a replacement for fishmeal (FM) in the juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) was investigated concerning differences in growth performance, immune response, and intestinal microbiota among the dietary treatments. Hybrid grouper were fed a diet (isonitrogenous and isolipidic) with FM or FM replaced

Along with the intensification of culture systems to meet the increasing global demands, there is an elevated risk of disease outbreak and substantial loss for shrimp farmers. Probiotics, e.g., Lactobacillus plantarum, are among the most promising feed supplements for controlling or treating microbial diseases in shrimp aquaculture. The maintenance of live and viable probiotic bacterial culture during

Ghana's national annual fish deficit was 550,000 tons in 2018 leading to persistent increase in fish importation. However, capture fisheries have been declining over the years. Adoption of Aquaculture technologies has the potential to improve fish productivity, but the decision to adopt new technologies by fish farmers is rather complex and influenced by factors including socioeconomic and institutional

Distant hybridization is an important method for obtaining hybrids with hybrid genomes. In this study, a new type of hybrid culter (BTBT, 2n = 48) was produced by two rounds of backcrossing with an established hybrid lineage of blunt snout bream (Megalobrama amblycephala, BSB, 2n = 48, ♀) × topmouth culter (Culter alburnus, TC, 2n = 48, ♂). Analyses on DNA content, chromosome number, and fertility

Salmonid rickettsial septicaemia (SRS) is the most important disease of farmed salmonid fish in Chile and the main driver of a high rate of antimicrobial use. This study evaluated the effectiveness of antimicrobial treatment of SRS outbreaks, using industry-generated data from 8318 cage-level production cycles stocked between 2003 and 2018. We defined SRS outbreaks by a specified level of SRS-attributed

The fatty acyl elongases Elovl4 are pivotal components in the biosynthesis of very long-chain polyunsaturated fatty acids (VLC-PUFA) from long-chain polyunsaturated fatty acids (LC-PUFA). Thus, nutritional regulation of Elovl4, as well as other elongase and desaturase genes involved in LC-PUFA biosynthesis (e.g., Elovl5, Fads2) has been proposed as a strategy to enhance endogenous production of LC-PUFA

A 56-day feeding experiment was conducted to evaluate the dietary Lipidol™ (functional ingredient: lysophospholipids) on growth performance, biochemical and immunological parameters, and selected immune and antioxidant-relevant genes expression in rainbow trout. Fish (N = 840; 70.4 ± 1.5 g) were randomly distributed in 12 circular fiberglass tanks and nominated to four treatments: 0 (control), 1, 2

The aim of this research was to investigate the efficacy of seven different diets including live Acetes (LA), minced shrimp meat (MSM), locally formulated feed (LFF), a commercial feed (CF), and three combination diets of LA with MSM (LA + MSM), LFF (LA + LFF) and CF (LA + CF) on the nursery production of mud crab (Scylla paramamosain) crablets. To avoid the influence of cannibalism on the test diets

Microalgae are a crucial part in many aquaculture feed applications processes, mainly in hatcheries. Many aquaculture hatcheries maintain a small scale microalgae production facility in-house for the production of live feed. Microalgae are usually grown in non-automated bubble-column systems at unknown production costs. Other reactor systems or scenarios utilizing artificial light or sunlight and at

Mass mortality caused by pathogens has severely affected the oyster cultivation industry, but our knowledge about the innate immune system of oysters is still limited. The nuclear factor-κB (NF-κB) signaling pathway plays a vital role in many cellular, developmental, and biological processes. And inhibitor of NF-κB (IκB) proteins can prevent NF-κB nuclear localization and DNA binding, thereby inhibiting

Light is one of the most important structuring factors for life in aquactic biology. For zooplankton grazers, including copepods, light offers a behavioral dilemma that is potentially present at both small and large scales. We investigated the grazing rate, clearance rate, fecal pellet production rate, egg production rate, percertage of egg hatching and survival percentage of the freshwater copepod

Thailand was one of the largest seafood producers and exporters in the world; however, Thailand lost its number one position due to many drastic changes (e.g., EMS in shrimp, new laws and regulations, depletion of natural resources) in the past few decades. These changes have impacted the Supply Chain of Thai Seafood industry. Our research on Thailand's Seafood industry using several government's statistics

The croaker Cilus gilberti is an important coastal food fish in Chile and Peru, and is being considered as a target marine species for diversification of Chilean aquaculture. In order to optimize culture, responses to density and potential environmental rearing conditions must be examined. Stocking density (SD) is an important factor for cultivation-related economic viability, because inappropriate

The use of food additives is an important preventive approach that has proven to maintain the health status of fish, especially under stressful conditions. The dietary ferulic acid (FA) was included in the diets at 0, 20, 40, 80, and 160 mg per kg to investigate its beneficiary aspects on Nile tilapia. After 60 days, fish were exposed to heat stress (HS), then blood and liver samples were collected

This research was directed to examine the influences of dietary silymarin supplementation in growth, intestinal mucosal permeability, histology and apical junctional complex (AJC) (including tight junction (TJ) and adherents junction (AJ)), as well as the appropriate level of silymarin supplementation to juvenile grass carp (Ctenopharyngodon idella). Fish was fed at 6 graded levels of silymarin (0

Passive acoustics monitoring (PAM) is an exceptional tool to analyze the feeding behavior of shrimp, as they emit a “click” sound by the mandibular occlusion. Hence, acoustic demand-feeding systems based on the click signals energy have been applied to improve feeding management in shrimp farming. These acoustic signals may be affected by the texture properties of the foods pellets, which are modified

A 60-day feeding trial was conducted on Genetically Improved Farmed Tilapia (GIFT) juveniles in inland saline water (ISW) of 10 ppt salinity. Seven isoenergetic (400 kcal DE/100 g), isolipidic (6%) and hetero‑nitrogenous (20, 25, 30, 35, 40, 45 and 50% CP) experimental diets were formulated and prepared using purified ingredients. Three hundred and fifteen GIFT juveniles (average body weight 2.68 ± 0

Streptococcosis is a major disease that causes severe mortality in tilapia aquaculture worldwide. Although the conventional BLUP family selection to enhance disease resistance in a commercial red tilapia stock was successful, the response was low due to the low heritability of the traits. An alternative strategy is the utilization of genomic information to identify the best performing candidates within

Macrobrachium rosenbergii, the giant freshwater prawn, is an important aquaculture species in China and other Southeast Asian countries. Here, we report the positive effect of dietary betaine supplementation on growth performance and the innate immune system in M. rosenbergii. Four diets were formulated containing graded levels of betaine, and a 10-week feeding trial was conducted. Prawns fed a diet

Genetic improvement of tiger prawn Penaeus monodon is challenging due to difficulties in breeding and rearing of this species in captive environments. To date, there have been very limited quantitative genetic studies in this species, especially genotype by environment (G × E) interaction between diverse farming systems (e.g., tank vs. pond). Therefore, the present study ran the long-term captive selection

Leptin is an important energy homeostasis hormone in mammals. However, the role of leptin in energy balance of fish remains controversial. In this study, we characterized Yangtze sturgeon (Acipenser dabryanus) leptin (Adleptin) with a full-length 507 bp coding sequence (CDS) encoding 168 amino acids (aa) and we used phylogenetic analysis to identify it as leptin A. The tissue expression profiles suggested

For a long-term growth in shrimp farming, a significant increase in shrimp output can only be fully realized by improving both technical and cost efficiency. This study employs data envelopment analysis with the double bootstrapping technique to analyze cost and technical efficiency for aquaculture. This procedure allows statistical inferences about cost and technical efficiency to be made. The case

Hepatopancreatic necrosis syndrome (HPNS) has a severe impact on the Chinese mitten crab (Eriocheir sinensis) industry. In this work, a cross-sectional study method was used to assess the association between 55 variables and HPNS. A total of 98 farmers were surveyed, including 34 whose farms had crabs with HPNS (treated as the diseased group) and 64 whose farms did not have crabs with HPNS (treated

Bacterial enteritis caused by Aeromonas hydrophila is one of the most common diseases in grass carp culture. There have been intensive reports on grass carp after A. hydrophila infection at the molecular level, but analyses of the intestinal microbiota are rare. In this study, histopathological sections were examined, and 16S rRNA sequencing was used to study the changes in the intestinal tissue and

Common carp (Cyprinus carpio) is one of the most important aquaculture species in the world. In recent years, several outbreaks of unknown etiology has caused huge economic losses in common carp culture. To determine the death cause of common carp in Chongqing, China, we isolated potential pathogens from the infected common carp. The isolated bacteria (CQC01) recovered from moribund common carp was

In the present study, we evaluated the effects of a medicinal plant leaf extract (MPLE; 10%, ursolic acid, 3% other triterpenic compounds; 2% verbascoside and < 1% polyphenols) obtained from Lippia citriodora and Salvia officinalis on somatic growth and immune responses in juvenile gilthead seabream (Sparus aurata). Fish (initial body weight = 26.0 ± 0.1 g) were fed two isoproteic (48% crude protein

A strain of rainbow trout at the Hagerman Fish Culture Experiment Station has undergone a long-term selection program for growth on a plant-based diet (HGM strain). Comparing fish from the F8 generation to a commonly available commercial strain selected for growth (CSS), we sought to investigate potential differences in oral tolerance, intestinal microbiota richness and overall growth when fed a 40%

Lactobacillus pentosus HC-2 is a lactobacillus previous isolated and identified from aquatic animals (such as the goby, Acanthogobius hasta), which has shown a positive effect on growth and disease resistance in the Pacific white shrimp, Penaeus vannamei. Aflatoxin B1 (AFB1) is a highly carcinogenic and teratogenic toxin found in moldy feeds. This study aims to investigate the positive effect of L

Salmon lice, Lepeophtheirus salmonis are a financial and fish welfare issue for the salmon aquaculture industry. Avoiding the planktonic, mostly surface-dwelling infective stage of lice by holding salmon deep in submerged sea-cages could reduce host-parasite encounter rates, reducing the lice problem. However, submergence, or long-term lack of surface access (weeks to months) is not feasible for the

This study was conducted to evaluate the individual and combined effects of emodin and Clostridium butyricum on growth and non-specific immunity of giant freshwater prawn (Macrobrachium rosenbergii) for eight weeks with the initial body weight of 0.19 ± 0.5 g. Nine trial diets were prepared to contain three emodin levels (0, 25 and 50 mg/kg) and three C. butyricum levels (0, 250 and 500 mg/kg, 2 × 107 CFU/g)

The aim of the present study was to assess the effects of dietary oak (Quercus castaneifolia) leaf extract supplementation on growth performance, antioxidant, immune and stress responses in common carp (Cyprinus carpio). Ethanolic extract of the oak leaf were prepared and tested for in vitro radical scavenging and antibacterial properties. The results showed that the extract has radical scavenging

We examined the effect of rearing tank shape on survival and growth of Pacific bluefin tuna Thunnus orientalis larvae. Cylindrical (1.7 × 103 cm2 water surface area; 30 cm deep) and rectangular (1.8 × 103 cm2 water surface area; 28 cm deep) tanks (n = 3 each) were filled with 50 l of seawater. One air stone with a 100 ml/min aeration rate was set at the bottom center of each tank. Light intensity at

Light is an important environmental factor for aquatic organisms. In biofloc systems, light intensity and photoperiod length might also affect the performance and biomass of culture animals as well as the microbial communities. This study aimed to evaluate the impacts of light intensity on the nitrogen removal process, microbial communities and Litopenaeus vannamei performance at the commercial scale

The microRNAs (miRNAs) are known to regulate immune functions in crustaceans, but little is known about the role of miRNAs against bacterial infection. We performed small RNA sequencing to characterize the differentially expressed microRNAs in V. parahemolyticus infected crab, in comparison to that in control uninfected crab, at 12 h and 24 h post infection. In total, 23 host miRNAs were up-regulated

The Manila clam, Ruditapes philippinarum, is an economically and scientifically important bivalve mollusk. Its tolerance of aerial exposure has long been considered an important trait for its survival under acute environmental stress. In this study, the transcriptome sequencing of Manila clam under air exposure and reimmersion stresses were conducted, and the differential expression of stress responsive

Chemically induced anaesthesia is currently the only approved method for anaesthetising fish for sampling and surgical procedures by many organizations, including the Canadian Council on Animal Care, Health Canada, and the U.S. Food and Drug Administration (FDA). Although MS-222 (Tricaine-S) performs well, the long chemical withdrawal time makes it a poor choice for field-based research where fish

The objective of this study was to integrate the treatment of aquaculture effluent with the biomass and macronutrients production by Spirulina sp. LEB 18. The first experiment was carried out in a photobioreactor (1 L) and the best biomass production condition was reproduced on a pilot scale in a raceway type bioreactor (5 L). The best treatment was T25 (100% of wastewater supplemented with 25% of

This report describes the detailed microbial and histological characteristics of Vibrio parahaemolyticus strains associated with acute hepatopancreatic necrosis disease (VpAHPND) that infected Pacific white shrimp (Penaeus vannamei) cultured in Korea. Shrimps showing typical symptoms of AHPND, including a whitish hepatopancreas and empty gut, were reported at two private shrimp farms in Korea in 2019

Mortality outbreaks in adult oysters (Crassostrea gigas) have been reported since 1994 in Normandy. Recent work has shown that triploid oysters tend to be subject to higher mortality than diploids. Many parameters could be involved in this phenomenon including pathogens, environmental conditions, the physiological and genetic states of the oysters. With respect to reproductive physiology, studies showed

The objective of this study was to evaluate the immune response of Macrobrachium rosenbergii to acute stress, including the pathogen challenge, acute H2O2 and pH stress, to explore the application of biofloc systems in prawn farming. All 780 prawns (0.19 ± 0.01 g) were randomly distributed into one control group with 1/3 water changed daily (no carbon source addition) and three biofloc groups with

To develop plant-based diets for hybrid striped bass (HSB), Morone chrysops × M. saxatilis, we evaluated six soy products in extruded, ideal protein, fish meal (FM) replacement diets fed to soy-naïve juveniles (3.72 ± 0.01 g initial weight) for up to 5 months. The products included soy protein concentrate (SPC), a conventional soybean meal (SBM) from Archer Daniels Midland (ADM), three non-genetically

The first step for assessing and refining the nutritional requirements during the larval and early juvenile stages of a fish is the study of the ontogeny of digestive system functionality. The combination of these studies with ecological and anatomical knowledge of the species of interest establishes the base for facing one of the major aquaculture challenges: promoting larvae growth and survival.

This is the first post-USSR country case study which assesses the value chain performance of the aquafeed sub-sector including mapping the aquafeed value chain, performance of different actors in terms of value addition and profitability and their possible constraints, and feed regulations, institutions and policies of aquafeed sub-sector in Kyrgyz Republic. Qualitative and quantitative data were collected

A number of studies have highlighted the promising growth of Egyptian tilapia aquaculture and the role of genetically improved strains in this development, such as the Abbassa Nile tilapia (Oreochromis niloticus, Linneaus, 1758). However, few studies have explored the link between aquaculture development and changes in fish demand among low-income consumers. This study combines household budgeting

Aflatoxin B1 (AFB1) is a mycotoxin that is commonly detected in aquatic feed in tropical and subtropical regions. The toxic effects in shrimp and fish tissues following exposure to chronic AFB1 exposure has been studied but there is no information on tolerance between different species. This study was designed to compare the tolerance of AFB1 between invertebrate Litopenaeus vannamei and vertebrate

The yellowtail kingfish (Seriola lalandi) is a marine endemic fish, and a key species in the national programme for the diversification of Chilean aquaculture. Since it has been recently developed from wild fish, the biology of this species under production is to a large extent unknown. For example, the structure of the different populations used to create the national breeding programme is not well

Astaxanthin produced by Haematococcus pluvialis is an economically valuable ketocarotenoid pigment that benefits the health of human being. Astaxanthin can be accumulated in H. pluvialis cells up to 3% under high light stress. In this study, by adding 1 mM potassium iodide (KI) into the culture media of H. pluvialis cells under high light condition (HK), 26%–35% more astaxanthin than that of high light

The objective of this study was to obtain diagnostic performance estimates for immunofluorescence antibody test (IFAT) and quantitative polymerase chain reaction (qPCR) tests, for Bacterial Kidney Disease (BKD) surveillance in farmed Atlantic salmon (Salmo salar) broodstock without signs compatible with the disease in Chile. To obtain diagnostic sensitivity (DSe) and specificity (DSp) estimates of

Infectious pancreatic necrosis virus (IPNV) causes severe viral disease in salmonids, with major economic losses in the global rainbow trout aquaculture industry following from a high mortality rate. In the present study, we aimed to develop a safe and efficient vaccine to protect rainbow trout against IPNV infection. Administered via the immersion route, a live vector vaccine containing the coding

In a previous study, the frequency of three different trypsin genotypes of Penaeus vannamei in aquaculture was described. Genotype CBA was the most abundant, followed by genotype CB, but genotype CA decreased its frequency and was not found in shrimps higher than 5 g of body weight. The conclusion was that shrimp with genotype CA inexplicably die under culture conditions. The question was if the process