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iTRAQ-based proteomic analysis reveals the molecule mechanism of reducing higher alcohols in Chinese rice wine by nitrogen compensation Ann. Microbiol. (IF 1.528) Pub Date : 2021-01-14 Guidong Huang; Hong Ren; Ali Wang; Xinran Wan; Ziying Wu; Xianfeng Zhong
Higher alcohol is a by-product of the fermentation of wine, and its content is one of the most important parameters that affect and are used to appraise the final quality of Chinese rice wine. Ammonium compensation is an efficient and convenient method to reduce the content of higher alcohols, but the molecule mechanism is poorly understood. Therefore, an iTRAQ-based proteomic analysis was designed
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Biochemical and molecular changes of the zosteric acid-treated Escherichia coli biofilm on a mineral surface Ann. Microbiol. (IF 1.528) Pub Date : 2021-01-07 Federica Villa; Francesco Secundo; Fabio Forlani; Cristina Cattò; Francesca Cappitelli
The main goal of the present work was to assess the effectiveness of zosteric acid (ZA) in hindering Escherichia coli biofilm formation on a mineral surface. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) flow system was used to probe in situ the biochemical changes induced by ZA on E. coli sessile cells growing on the zinc selenide ATR plate. Comparative proteome analysis was conducted
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Draft genome sequences of two Streptomyces albidoflavus strains DEF1AK and DEF147AK with plant growth-promoting and biocontrol potential Ann. Microbiol. (IF 1.528) Pub Date : 2021-01-06 Andrea Kunova; Paolo Cortesi; Marco Saracchi; Guy Migdal; Matias Pasquali
Bacteria belonging to the Streptomyces genus can be exploited in environmentally friendly approaches to food safety. Genome information can help to characterize bioactive strains opening the possibility to decipher their mechanisms of action. The biocontrol and plant growth-promoting activity of two Streptomyces spp. strains, DEF1AK and DEF147AK, were assessed in vitro and in planta. The genome sequences
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The complex microbiome in aquaponics: significance of the bacterial ecosystem Ann. Microbiol. (IF 1.528) Pub Date : 2021-01-04 Nasser Kasozi; Benjamin Abraham; Horst Kaiser; Brendan Wilhelmi
Aquaponics is a technology that has minimal impact on the environment and which is often promoted as a solution for sustainable food production. Developing aquaponics sustainably requires a thorough understanding of the biological components of the system. Recent reports on the bacterial populations of aquaponics systems using new DNA sequencing technologies are revealing a complex and diverse microbial
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High-level production of γ-cyclodextrin glycosyltransferase in recombinant Escherichia coli BL21 (DE3): culture medium optimization, enzymatic properties characterization, and product specificity analysis Ann. Microbiol. (IF 1.528) Pub Date : 2020-12-14 Menglu Duan; Yan Wang; Guowu Yang; Jiao Li; Yi Wan; Yuan Deng; Yong Mao
γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications. Therefore, the present study aimed at optimizing an economical
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Combined effect of water activity and pH on the growth of food-related ascospore-forming molds Ann. Microbiol. (IF 1.528) Pub Date : 2020-12-14 Irene Racchi; Nicoletta Scaramuzza; Alyssa Hidalgo; Elettra Berni
The contamination of raw materials, packaging, or processing environments by fungal ascospores is a real concern for food industries, where variable rates of spoilage can be reached in pasteurized acidic products such as fruit juices, fruit jams, or soft drinks. The aim of this work was to assess the combined effect of aw and pH on the growth of six isolates from three genera of ascospore-forming molds
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Molecular characterization of cellulolytic (endo- and exoglucanase) bacteria from the largest mangrove forest (Sundarbans), Bangladesh Ann. Microbiol. (IF 1.528) Pub Date : 2020-12-07 Satyajit Biswas; Md. Al Saber; Ismoth Ara Tripty; Md. Adnan Karim; Md. Aminul Islam; Md. Shazid Hasan; A. S. M. Rubayet Ul Alam; Md. Iqbal Kabir Jahid; Md. Nazmul Hasan
Cellulase, due to its massive applicability, has been used in various industrial processes such as biofuels (bioethanol, triphasic biomethanation), agricultural and plant waste management, chiral separation, and ligand binding studies. The finding of a novel cellulase-producing bacterium will benefit the industries, which rely on yeast to produce cellulase in fermentation technology, because bacteria
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Production and partial characterization of a crude cold-active cellulase (CMCase) from Bacillus mycoides AR20-61 isolated from an Alpine forest site Ann. Microbiol. (IF 1.528) Pub Date : 2020-11-25 Elisa Steiner; Rosa Margesin
To evaluate the production of a cold-active CMCase (endoglucanase) by Bacillus mycoides AR20-61 isolated from Alpine forest soil and to characterize the crude enzyme. After studying the effect of cultivation parameters (medium composition, temperature, NaCl concentration, pH) on bacterial growth and enzyme production, the crude enzyme was characterized with regard to the effect of pH, temperature,
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Differences in the bacterial profiles and physicochemical between natural and inoculated fermentation of vegetables from Shanxi Province Ann. Microbiol. (IF 1.528) Pub Date : 2020-11-25 Zhidi Chen; Jianyi Kang; Yao Zhang; Xinxin Yi; Xiaona Pang; Hongmei Li-Byarlay; Xiuzhi Gao
Fermented vegetables can be divided into two types, natural fermented and artificially inoculated fermented. By detecting and identifying the changes of bacterial diversity using physical and chemical indicators during natural and inoculation fermentation, we analyzed and determined the dominant bacteria in the fermentation process and revealed the relationship between bacteria and volatile substances
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Gut microbiota and the human gut physiological changes Ann. Microbiol. (IF 1.528) Pub Date : 2020-11-23 Ousman Bajinka; Alansana Darboe; Yurong Tan; Khalid A. Abdelhalim; Lamin B. Cham
The human gut can be colonized by number of microorganisms. The most studied are bacteria, which changes from birth to newborn born into adult-like gut microbiota. Much is known about the effects of dietary, medications, and lifestyles on the bacterial composition. However, the host physiological changes influencing the gut microbiota, the immediate consequences, and the possible gut microbiota therapy
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Microbiology can make a great contribution to the new direction of global politics Ann. Microbiol. (IF 1.528) Pub Date : 2020-11-23 Claudia Sorlini
Our planet is currently being challenged by climate change which is causing severe consequences on the environment, economy, and social wellbeing. The main cause is the use of non-renewable energy sources, used in all anthropogenic activities. To this emergency, the problems of pollution are added. In particular, plastics that invade the planet are the most blatant phenomenon, and the nonsensical use
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Microbial community analysis with a specific statistical approach after a record breaking snowfall in Southern Italy Ann. Microbiol. (IF 1.528) Pub Date : 2020-11-09 Pamela Monaco; Fabio Divino; Gino Naclerio; Antonio Bucci
Snow and ice ecosystems present unexpectedly high microbial abundance and diversity. Although arctic and alpine snow environments have been intensively investigated from a microbiological point of view, few studies have been conducted in the Apennines. Accordingly, the main purpose of this research was to analyze the microbial communities of the snow collected in two different locations of Capracotta
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Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency Ann. Microbiol. (IF 1.528) Pub Date : 2020-10-31 Xiujie Gong; Hongtao Zou; Chunrong Qian; Yang Yu; Yubo Hao; Liang Li; Qiuju Wang; Yubo Jiang; Juntao Ma
The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization. According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary
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Effect of transgenic cotton continuous cropping on soil bacterial community Ann. Microbiol. (IF 1.528) Pub Date : 2020-10-29 Wen-hui Tian; Xiao-long Yi; Shan-shan Liu; Chao Zhou; Ai-ying Wang
In agricultural practices, continuous cultivation of genetically modified crops with high commercial value has a definite impact on soil microbial diversity. Soil microorganisms directly define the operational degree and function realization of the soil ecosystem. To understand the safety of environmental release, we studied the effects of continuous cropping of transgenic cotton on the diversity of
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Isolation of Arcobacter spp. and identification of isolates by multiplex PCR from various domestic poultry and wild avian species Ann. Microbiol. (IF 1.528) Pub Date : 2020-10-22 Elif Çelik; Salih Otlu
The purpose of the present study was to determine the extent and seasonal prevalence of Arcobacter spp. in domestic poultry and wild birds in the Kars region of Turkey using multiplex polymerase chain reaction (m-PCR). In this study, 1570 samples were collected from domestic poultry and wild avian species. The numbers of collected samples were as follows: 182 fecal samples from chickens, geese, and
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Correction to: Ochratoxigenic fungi and Ochratoxin A determination in dried grapes marketed in Tunisia Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-30 Samir Chebil; Wafa Rjiba-Bahri; Souheib Oueslati; Hanen Ben Ismail; Anis Ben-Amar; Pantelis Natskoulis
An amendment to this paper has been published and can be accessed via the original article.
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Evaluation of bacterial diversity during fermentation process: a comparison between handmade and machine-made high-temperature Daqu of Maotai-flavor liquor Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-25 Qiancheng Zuo; Yongguang Huang; MinGuo
High-temperature Daqu is a traditional fermentation starter that is used for Chinese Maotai-flavor Baijiu production. Although the bacteria in high-temperature Daqu are known to be responsible for developing the quality and flavor of Baijiu during the fermentation process, there is little information on the properties of the bacteria during the fermentation of high-temperature Daqu, especially machine-made
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Production of probiotic Mozzarella cheese by incorporating locally isolated Lactobacillus acidophilus Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-25 Hamid Mukhtar; Saima Yaqub; Ikram ul Haq
The present study was conducted to isolate and screen the potential probiotic strains for incorporation in Mozzarella cheese. Probiotic cultures were isolated from different randomly purchased yogurt samples and were identified as Lactobacillus sp., Bifidobacteria sp., and Pediococcus sp. after morphological and biochemical characterization. Heat tolerance of isolates was tested at 55 °C and 65 °C
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Liver-specific knockdown of ANGPTL8 alters the structure of the gut microbiota in mice Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-25 Yinlong Cheng; Yining Li; Yonghong Xiong; Yixin Zou; Siyu Chen; Wenxiang Zhang; Chang Liu; Yujie Shi
To investigate the effect of liver-specific knockdown of ANGPTL8 on the structure of the gut microbiota. We constructed mice with liver-specific ANGPTL8 knockdown by using an adeno-associated virus serotype 8 (AAV8) system harbouring an ANGPTL8 shRNA. We analysed the structure and function of the gut microbiome through pyrosequencing and KEGG (Kyoto Encyclopedia of Genes and Genomes) functional prediction
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Role of protein repair enzymes in oxidative stress survival and virulence of Salmonella Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-17 Arijit Shome; Ratanti Sarkhel; Shekhar Apoorva; Sonu Sukumaran Nair; Tapan Kumar Singh Chauhan; Sanjeev Kumar Bhure; Manish Mahawar
Proteins are the principal biomolecules in bacteria that are affected by the oxidants produced by the phagocytic cells. Most of the protein damage is irreparable though few unfolded proteins and covalently modified amino acids can be repaired by chaperones and repair enzymes respectively. This study reviews the three protein repair enzymes, protein l-isoaspartyl O-methyl transferase (PIMT), peptidyl
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Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440 Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-14 Lilia I. López-Lara; Laura A. Pazos-Rojas; Lesther E. López-Cruz; Yolanda E. Morales-García; Verónica Quintero-Hernández; Jesús de la Torre; Pieter van Dillewijn; Jesús Muñoz-Rojas; Antonino Baez
Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when desiccated cells are rehydrated for at least 24 h. To determine which genes of transport, oxidation-reduction
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Pyrosequencing scrutiny of bacterial and fungal communities in two Sudanese sorghum-based fermented foods Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-04 Mohamed Mutasim Eltayeb; Sara Ahmed Eltigani; Takeshi Taniguchi
Several fermented foods are produced from sorghum flour in Africa, especially in the dryland regions where it is the principal crop. It is important to explore the microbial communities in these foods to improve their quality and organoleptic properties. In this study, the bacterial and fungal communities of two popular sorghum-based fermented foods in Sudan (kisra and hulumur) were analyzed, for the
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Toxicity evaluation of Aphidicidal crystalliferous toxins from Bacillus thuringiensis strains: a molecular study Ann. Microbiol. (IF 1.528) Pub Date : 2020-09-02 Asokan Ramasamy; Meghana Suresh; Mahadeva Swamy Hanchipura Mallesh
Aphis gossypii and A. punicae are the most damaging pests. The emergence of large populations has created concern among farmers because this pest complex is considered critical as it has a significant effect on major crops around the globe. The lack of new technologies for the control of A. gossypii and A. punicae is also worrying due to the indiscriminate use of chemical insecticides. Besides, this
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Bacterial laccase of Anoxybacillus ayderensis SK3-4 from hot springs showing potential for industrial dye decolorization Ann. Microbiol. (IF 1.528) Pub Date : 2020-08-14 Jingjing Wang; Fei Chang; Xiaoqing Tang; Wei Li; Qiang Yin; Yang Yang; Yang Hu
Laccases are green biocatalysts that possess attractive for the treatment of resistant environmental pollutants and dye effluents. To exploit the laccase of Anoxybacillus ayderensis SK3-4 that possesses dye decolorization ability at room and higher temperature, we characterized the enzyme in considerable detail and investigated its ability to decolorize different dyes. A bacterial laccase gene designed
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Tracking strategic developments for conferring xylose utilization/fermentation by Saccharomyces cerevisiae Ann. Microbiol. (IF 1.528) Pub Date : 2020-08-07 Shalley Sharma; Anju Arora
Efficient ethanol production through lignocellulosic biomass hydrolysates could solve energy crisis as it is economically sustainable and ecofriendly. Saccharomyces cerevisiae is the work horse for lignocellulosic bioethanol production at industrial level. But its inability to ferment and utilize xylose limits the overall efficacy of the process. Data for the review was selected using different sources
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Effects of inorganic and organic treatments on the microbial community of maize rhizosphere by a shotgun metagenomics approach Ann. Microbiol. (IF 1.528) Pub Date : 2020-07-31 Matthew Chekwube Enebe; Olubukola Oluranti Babalola
The main drivers of biogeochemical cycling of nutrients, plant growth promotion, and disease suppression are microbes. Organic manure increases soil quality and plant productivity; the same is true of inorganic fertilizer. In this study, we explored shotgun metagenomics study to investigate how maize (Zea mays everta) rhizosphere microbial community diversity is shaped following the application of
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Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalga Dunaliella salina Y6 Ann. Microbiol. (IF 1.528) Pub Date : 2020-07-25 Mingcan Wu; Rongfang Zhu; Jiayang Lu; Anping Lei; Hui Zhu; Zhangli Hu; Jiangxin Wang
Under different abiotic-stress conditions, the unicellular green microalga Dunaliella salina accumulates large amounts of carotenoids which are accompanied by fatty acid biosynthesis. Carotenoids and fatty acids both possess long carbon backbones; however, the relationship between carotenoid and fatty acid metabolism is controversial and remains poorly understood in microalgae. In this study, we investigated
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Different fertilization treatments in coal mining-affected soils change bacterial populations and enable soil reclamation Ann. Microbiol. (IF 1.528) Pub Date : 2020-07-23 Yanzhuan Cao; Bingrui Zhou; Xiangying Wang; Huisheng Meng; Jian Zhang; Li Li; Jianping Hong
Coal mining activities result in large-scale soil degradation and ecosystem imbalances in many countries. Fertilization is an effective way to improve soil fertility and microbial activity. However, the effect of different fertilizers and remediation time on the subsided soil is not clear. The aim of this study is to explore the effects on soil fertility and the bacterial community. In this study,
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Complete genome sequence of butenyl-spinosyn-producing Saccharopolyspora strain ASAGF58 Ann. Microbiol. (IF 1.528) Pub Date : 2020-07-08 Chao Guo; Weiqun Guo; Yuchun Liu; Chao Wang
This study aimed to analyze the complete genome sequence of the butenyl-spinosyn-producing strain Saccharopolyspora sp. ASAGF58, isolated from Zhejiang province. PacBio RS II sequencing platform with single-molecule real-time technology was used to obtain the complete genome sequence of Saccharopolyspora sp. ASAGF58. Gene prediction and annotation analysis were carried out through several software
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Determination of steroid hydroxylation specificity of an industrial strain Aspergillus ochraceus TCCC41060 by cytochrome P450 gene CYP68J5 Ann. Microbiol. (IF 1.528) Pub Date : 2020-07-06 Xue Wang; Xingwei Yang; Xi Jia; Peng Jin; Zhengxiang Wang; Fuping Lu; Xiaoguang Liu
The use of Aspergillus ochraceus TCCC41060 for synthesis of 11α-OH-ethylgonendione, an important intermediate for synthesis of desogestrel-a major ingredient of the “third-generation” oral contraceptives, is hampered by its low regioselectivity of hydroxylation. In the present study, we sought to characterize gene(s) involved in steroid hydroxylation specificity in strain TCCC41060. Taking advantage
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Impact of different temperature profiles on simultaneous yeast and bacteria fermentation Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-29 Raffaele Guzzon; Tomas Roman; Roberto Larcher
The role of fermentation temperature was studied for its impact on the evolution of malolactic fermentation performed by simultaneous inoculum of yeast and bacteria in grape must. Results were discussed considering the different fermentative kinetics and the composition of obtained wines. Two strains of bacteria belonging to the O. oeni and L. plantarum species were inoculated 24 h after the beginning
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Critical threat associated with carbapenem-resistant gram-negative bacteria: prioritizing water matrices in addressing total antibiotic resistance Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-25 Anthony A. Adegoke; Opeyemi K. Fatunla; Anthony I. Okoh
The World Health Organization (WHO) in 2017 classified some carbapenem-resistant Gram-negative bacteria into a critical criterial group for research and development. This study reviews the need to prioritize the water matrices as hotspot in the development and transfer of antibiotic resistance determinants, where future total resistant superbug may emerge. Published articles on Google Scholars, PubMed/Medline
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Anaerobic methane-oxidizing bacterial communities in sediments of a drinking reservoir, Beijing, China Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-25 Yang Liu; Xiaoyan Wang; Yongjuan Chen; Lingrong Zhang; Kangli Xu; Yi Du
Nitrate/nitrite-dependent anaerobic methane oxidation (N-DAMO) connects the global cycle of carbon and nitrogen in aquatic ecosystems. The aims of this study were to investigate the spatiotemporal variation of N-DAMO bacteria and its driving factors in a drinking reservoir which are strongly affected by human consumption. Cloning analyses were used to study the pmoA and 16S rRNA genes of N-DAMO bacteria
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Life in mine tailings: microbial population structure across the bulk soil, rhizosphere, and roots of boreal species colonizing mine tailings in northwestern Québec Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-24 Vanessa Gagnon; Michaël Rodrigue-Morin; Julien Tremblay; Jessica Wasserscheid; Julie Champagne; Jean-Philippe Bellenger; Charles W. Greer; Sébastien Roy
Mining activities have negative effects on soil characteristics and can result in low pH, high heavy metal content, and limited levels of essential nutrients. A tailings storage area located in northwestern Québec showed natural colonization by plants from the adjacent natural environment. The objective of the study was to determine the main edaphic parameters that structured microbial populations
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Rapid identification and detection of Vibrio parahaemolyticus via different types of modus operandi with LAMP method in vivo Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-24 Jun Li; Jianfei Liu; Kaifei Fu; Kewei Qin; Chenglin Wu; Xiaojie Yu; Shan Zhou; Lijun Zhou
Vibrio parahaemolyticus, an easy-ignored food-borne pathogen, can cause bacterial outbreaks and human disease during early-stage infection. In this study, we aimed to evaluate the detection efficiency of loop-mediated isothermal amplification (LAMP) as an emerging technique to directly detect V. parahaemolyticus infection in mammalian hosts and assess its potential in clinical applications. A LAMP
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Effects of continuous cropping of sugar beet (Beta vulgaris L.) on its endophytic and soil bacterial community by high-throughput sequencing Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-22 Miao Li; Fuzhen Yang; Xianyu Wu; Hai Yan; Yang Liu
As a major sugar crop, sugar beet (Beta vulgaris L.) plays an important role in both sugar industry and feed products. Soil, acts as the substrate for plant growth, provides not only nutrients to plants but also a habitat for soil microorganisms. High soil fertility and good micro-ecological environment are basic requirements for obtaining high-yield and high-sugar sugar beets. This study aimed at
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Ochratoxigenic fungi and Ochratoxin A determination in dried grapes marketed in Tunisia Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-16 Samir Chebil; Souheib Oueslati; Anis Ben-Amar; Pantelis Natskoulis
With the present work, we aimed to assess the occurrence of ochratoxigenic fungi and Ochratoxin A (OTA) in dried grapes from Tunisia. Dried grapes samples (n = 90) were investigated for the presence of ochratoxigenic fungi, which were further characterized at the species level through amplification of the internal transcribed spacer (ITS) region and polymerase chain reaction (PCR) product sequencing
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The bacterial communities of Tuber aestivum: preliminary investigations in Molise region, Southern Italy Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-16 Pamela Monaco; Marwene Toumi; Gabriella Sferra; Erika Tóth; Gino Naclerio; Antonio Bucci
Truffles are colonized by a complex microbial community of bacteria, yeasts, and filamentous fungi, whose role has not yet been fully understood. The main purpose of the research was to characterize the bacterial communities associated with Tuber aestivum Vittad. fruiting bodies collected from natural truffle grounds in the Molise region (Southern Italy). Despite it is one of the Italian richest areas
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Diagnosing bioremediation of crude oil-contaminated soil and related geochemical processes at the field scale through microbial community and functional genes Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-15 Pingping Cai; Zhuo Ning; Yaci Liu; Ze He; Jiansheng Shi; Manlan Niu
Bioremediation is widely considered the most desirable procedure for remediation of oil-contaminated soil. Few studies have focused on the relationships among microbial community, functional genes of biodegradation, and geochemical processes during field bioremediation, which provide crucial information for bioremediation. In the current study, the microbial community and functional genes related to
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First report of Wolbachia in Damaeus onustus (Acari: Oribatida) Ann. Microbiol. (IF 1.528) Pub Date : 2020-06-15 Edyta Konecka; Ziemowit Olszanowski; Aleksandra Jagiełło
Little is known about the distribution and phylogeny of bacterial endosymbionts in oribatid mites (Acari: Oribatida). Thus, we undertook the issue of occurrence of these microbial symbionts in this arthropod group. We used PCR technique for detection of Wolbachia in Damaeus onustus. Phylogenetic analysis of the bacterium was conducted based on the 16S rDNA sequence. To the best of our knowledge, we
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Bacterial community characterization of the rhizobiome of plants belonging to Solanaceae family cultivated in desert soils Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-29 Aarón Barraza; María Goretty Caamal-Chan; Thelma Castellanos; Abraham Loera-Muro
The plant Solanaceae family is one of the most important for global agriculture and nutrition. Within this plant family, two plant species stand out for their economic importance and for human consumption, which are tomato (Solanum lycopersicum) and chili pepper (Capsicum annuum). Moreover, those plants support diverse and characteristic microbial communities that are uniquely suited to the plant habitat
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Diversity of hydrolase-producing halophilic bacteria and evaluation of their enzymatic activities in submerged cultures Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-27 Leila Batsoule Drissi Kaitouni; Jaouad Anissi; Khalid Sendide; Mohammed El Hassouni
In this work, we assessed the diversity of culturable halophilic bacteria that produce hydrolytic enzymes from both natural and artificial hypersaline regions in the pre-Rif region of Morocco. Bacteria were isolated from three hypersaline sites, in solid medium supplemented with various salt concentrations ranging from 0 to 330 g/L. Physical and chemical characteristics of samples from the isolation
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Expression of a highly active β-glucosidase from Aspergillus niger AS3.4523 in Escherichia coli and its application in gardenia blue preparation Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-19 Shuai Hao; Yuanpu Liu; Yu Qin; Lei Zhao; Jiawen Zhang; Tingting Wu; Baoguo Sun; Chengtao Wang
Gardenia blue is one of the natural food additives used in East Asia for many years. Its biosynthesis relies on a key rate-limiting cellulase: β-glucosidase (BGL), which mainly exists in Aspergillus niger (A. niger) cells. The purpose of this study was to obtain active β-glucosidase by cell engineering method and applied to gardenia blue synthesis, which would help to promote the application and reduce
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Regulation and analysis of the diversity of intestinal microbiota in SD rats by Danggui Buxue Tang (DBT) fermented with Bacillus subtilis Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-15 Keyuan Chen; Yan Gao; Xinjian Wang; Feng Cheng; Shahbaz Ul Haq; Yun Liang; Zhiwei Yang; Bo Li; Yuan Liu; Yonggang Wang; Jianping Liang; Baocheng Hao
To investigate the effect of Danggui Buxue Tang (DBT) on intestinal microbiota diversity after fermentation by Bacillus subtilis. B. subtilis was used to ferment DBT. Sprague Dawley (SD) rats were randomly divided into the following four groups with six rats in each group: the control group, DBT nonfermentation group, B. subtilis group, and DBT fermentation group. Rats were fed continuously for 14
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Endophytic fungal community in grape is correlated to foliar age and domestication Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-15 Yanchun Fan; Linlin Gao; Pingping Chang; Zhi Li
The composition of endophytic communities has been shown to depend on grape genotypes and viticultural managements in leaves, stems, and berries of grape, but there have been relatively few reports exploring fungal endophytes associated with wild grape and foliar age. The regions of internally transcribed spacer (ITS) were sequenced using the Illumina HiSeq to determine the diversity of fungal endophytes
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The complete genome sequence of the archaeal isolate Halomicrobium sp. ZPS1 reveals the nitrogen metabolism characteristics under hypersaline conditions Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-15 Xinyu Hu; Chunlin Zeng; Zhaozhi Hou; Yuan Wang; Qin Xu; Kazuo Isobe; Keishi Senoo; Lin Zhu
As a potential tool for the biodegradation of nitrogen contaminants, including nitrate, nitrite, and ammonium, in pickled foods with high salinity, the halophilic and denitrifying archaeal strain Halomicrobium sp. ZPS1 was isolated from edible salt particles. Under anaerobic and static culture conditions, Halomicrobium sp. ZPS1 could simultaneously degrade nitrate, nitrite, and ammonium in liquid medium
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Production, purification, and characterization of cellulase from Acinetobacter junii GAC 16.2, a novel cellulolytic gut isolate of Gryllotalpa africana, and its effects on cotton fiber and sawdust Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-14 Sandipan Banerjee; Tushar Kanti Maiti; Raj Narayan Roy
The study aims to search for potent cellulase producer from the gut of Gryllotalpa africana as well as to characterize and determine the effect of the purified enzyme on the cellulosic waste materials. The potent cellulolytic strain was identified through morphological, biochemical, physiological, and molecular characterization like 16S rRNA and fatty acid methyl ester profile. After the optimization
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Oleaginous yeasts isolated from traditional fermented foods and beverages of Manipur and Mizoram, India, as a potent source of microbial lipids for biodiesel production Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-14 Pritam Bardhan; Kuldeep Gupta; Sumit Kishor; Pronobesh Chattopadhyay; Chayanika Chaliha; Eeshan Kalita; Vaibhav V. Goud; Manabendra Mandal
Oleaginous yeasts can accumulate intracellular lipid bodies or triacylglycerides (TAGs) under nutrient limiting conditions. TAGs derived from those yeast strains are considered as an alternative to conventional plant-based oils for biodiesel production. In this study, we attempt to isolate and characterize yeast strains from selected traditional fermented foods of Manipur and Mizoram, India, and study
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Abundance, diversity, and distribution patterns along with the salinity of four nitrogen transformation-related microbes in the Yangtze Estuary Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-04 Lei Zheng; Tingting Liu; Dongdan Yuan; Huipeng Wang; Shurong Zhang; Aizhong Ding; En Xie
The abundance and composition of nitrogen transformation-related microbes with certain environmental parameters for living conditions provide information about the nitrogen cycle in the Yangtze Estuary. The aim of this study was to explore the impacts of salinity on four N-related microbes and reveal the phylogenetic characteristics of microorganisms in the Yangtze Estuary ecosystem. A molecular biology
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A real-time immunocapture PCR (RT-IPCR) without interference of protein A for convenient detection of staphylococcal enterotoxin B from food and environmental samples Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-04 Bhairab Mondal; Shylaja Ramlal; Keerthana Setlem; Ashrita Mahadeva; Shruthi Aradhya; Manmohan Parida
A real-time immunocapture PCR (RT-IPCR) has been fabricated for the detection of Staphylococcus aureus enterotoxin B (SEB) from food and environmental samples. Considering the fact, anti-SEB immunoglobulin G (IgG) has affinity towards protein A, produced by nearly all S. aureus, and generates false-positive read out in all immuno-based assay. We have employed avian anti-SEB antibody (SEB-IgY) as capture
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The use of cyclic dipeptide producing LAB with potent anti-aflatoxigenic capability to improve techno-functional properties of clean-label bread Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-04 Maryam Ebrahimi; Alireza Sadeghi; Seyed Ali Mortazavi
The aim of the present study was to evaluate the antifungal activity, anti-aflatoxigenic capability, and technological functionality of the selected lactic acid bacteria (LAB) isolated from wheat sourdough. The preselected LAB isolates were screened based on their antifungal activities and acidification capacities. Then, the antifungal compounds were identified using gas chromatography/mass spectrometry
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Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system Ann. Microbiol. (IF 1.528) Pub Date : 2020-05-04 Sérgio Dantas de Oliveira Júnior; Carlos Eduardo de Araújo Padilha; Estefani Alves de Asevedo; Gorete Ribeiro de Macedo; Everaldo Silvino dos Santos
In this study, an aqueous two-phase micellar system (ATPMS), formed by the non-ionic surfactant Triton X-114, was used to investigate the partitioning of cellulolytic enzymes produced by the filamentous fungus Aspergillus fumigatus CCT 7873. Performance of the ATPMS on the partitioning of CMCase (activity on carboxymethyl cellulose) and FPase (activity on filter paper) was investigated by varying the
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Application of propidium monoazide coupled with quantitative PCR to evaluate cell viability of Bifidobacterium animalis subsp. lactis in a non-dairy probiotic beverage Ann. Microbiol. (IF 1.528) Pub Date : 2020-04-30 Carolinne Odebrecht Dias; Mirella Crhistine Scariot; Renata Dias de Mello Castanho Amboni; Ana Carolina Maisonnave Arisi
In this study, a PMA-qPCR assay was developed for the enumeration of Bifidobacterium animalis subsp. lactis BB-12 viable cells in a non-dairy probiotic beverage. Probiotic viability was monitored in three formulations of probiotic passion fruit juice microencapsulated by spray drying, during 30 days of storage at 4 °C. Viable cells were quantified using qPCR and PMA-qPCR assays targeting tuf gene and
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Isolation of two iron-reducing facultative anaerobic electricigens and probing the application performance in eutrophication water Ann. Microbiol. (IF 1.528) Pub Date : 2020-04-30 Xia Zhang; Hongpei Zhang; Chuan Wang; Qianru Chen; Yuqing Zhao; Qiaohong Zhou; Zhenbin Wu
Sediment microbial fuel cell (SMFC) is a promising bioremediation technology in which microbes play an important role. Electricigens as the bio-catalysts have effect on pollution control and electricity generation. It is of great significance to screen the microorganisms with the ability of generating electricity. The SMFC anode biofilm was used as microbiological source to study the feasibility of
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Evaluation of the probiotic attributes of Bacillus strains isolated from traditional fermented African locust bean seeds (Parkia biglobosa), “daddawa” Ann. Microbiol. (IF 1.528) Pub Date : 2020-04-30 Tochukwu Nwamaka Nwagu; Chika Jude Ugwuodo; Chukwudi O. Onwosi; Ogechukwu Inyima; Oluoma Chizaram Uchendu; Chioma Akpuru
The involvement of probiotic cultures in food fermentation guarantees enhanced organoleptic properties and maximum consumer health benefits. In this study, isolated Bacillus cultures used in the fermentation of African locust bean seeds “Parkia biglobosa” into the food condiment “daddawa” were evaluated for probiotic attributes. Bacillus cereus strains BC1 and BC2 were tested for tolerance to acid
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Inter domain interactions influence the substrate affinity and hydrolysis product specificity of xylanase from Streptomyces chartreusis L1105 Ann. Microbiol. (IF 1.528) Pub Date : 2020-03-30 Ke Xiong; Zi xiang Yan; Jia yun Liu; Peng gang Pei; Lei Deng; Le Gao; Bao guo Sun
This study investigated the influence of inter-domain interactions on the substrate affinity and hydrolysis product specificity of xylanase. Genes encoding a GH10 endo-xylanase from Streptomyces chartreusis L1105 xynA and its truncated derivative were cloned and expressed in Escherichia coli. The catalytic activities of the enzyme (xynA) and the derivative xynADCBM, lacking the carbohydrate binding
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Microbial community diversity of traditional dough starter (Jiaozi) from two provinces in northwest China Ann. Microbiol. (IF 1.528) Pub Date : 2020-03-19 Ting Liu; Jiamu Kang; Liu Liu; Xinzhong Hu; Xiaolong Wang; Xiaoping Li; Zhen Ma; Tian Ren
Microbial community composition is crucial for the flavor and quality of fermented foods. However, the microbiota of Chinese traditional dough starter (Jiaozi) from different origins has scarcely been studied. The aim of this study was to determine the composition of bacterial and fungal communities in six Jiaozi collected from two provinces in northwest China. Our study determined the composition
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Correction to: Production of high-value bioproducts enriched with γ-linolenic acid and β-carotene by filamentous fungi Umbelopsis isabellina using solid-state fermentations Ann. Microbiol. (IF 1.528) Pub Date : 2020-03-12 Ondrej Slaný; Tatiana Klempová; Slavomír Marcinčák; Milan Čertík
Following publication of the original article (Tao et al. 2020), the authors reported a missing funding source in the ‘Acknowledgements’ section.
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Probiotic potential of lyophilized Lactobacillus plantarum GP Ann. Microbiol. (IF 1.528) Pub Date : 2020-03-09 Satyamitra L. Shekh; Aarti A. Boricha; Jony G. Chavda; Bharatkumar Rajiv Manuel Vyas
Freeze drying of Lactobacillus plantarum GP in the presence of wall materials to achieve improved survival and retention of probiotic functionality during storage. L. plantarum cells were lyophilized in the presence of inulin, fructooligosaccharides, lactulose, and/or skim milk. The lyophilized vials were stored at 8–10 °C up to 6 months and cells from these vials were evaluated for their probiotic
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Comparative analysis of structural and functional aspects of phytoene synthase from Meiothermus taiwanensis strain RP Ann. Microbiol. (IF 1.528) Pub Date : 2020-03-09 Trinetra Mukherjee; Subhra Kanti Mukhopadhyay
The aim of this study is the in silico characterization of the structure and function of the phytoene synthase (PSY) of a red carotenoid producing thermophile Meiothermus taiwanensis strain RP with a comparative approach. PSYs from M. taiwanensis strain RP and other groups of thermophilic, mesophilic and psychrophilic bacteria, plants, protozoa, and algae were analyzed by ExPASy ProtParam, NCBI Conserved
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